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Conserved domains on  [gi|1131868762|emb|SIS70522|]
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hypothetical protein/tRNA(fMet)-specific endonuclease VapC [Belliella pelovolcani]

Protein Classification

type II toxin-antitoxin system VapC family toxin( domain architecture ID 10177446)

type II toxin-antitoxin (TA) system VapC family toxin functions as a ribonuclease that is neutralized by its cognate VapB family antitoxin

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PIN_VapC4-5_FitB-like cd09881
VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae ...
 7-128 8.93e-31

VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae FitB and related proteins; This family includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This family belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


:

Pssm-ID: 350229  Cd Length: 127  Bit Score: 106.80  E-value: 8.93e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   7 CLDTSVLLDFFRKKDRDKT-FLNQLLEEfdEIEISSITEFEIRYGIN-----ENQSQFWDQLFENVNVLPFDSESAKKAV 80
Cdd:cd09881     1 LLDTNICIDLLRGRPPSVLeRLEELLPE--DLAISSITLAELLYGAEksqrpEKNLAALEAFLSRLEVLPFDEEAAEIYG 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1131868762  81 EIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLELL 128
Cdd:cd09881    79 EIRAELEKKGKPIGDNDLLIAATALAHGLTLVTNNTKHFERIPGLKVE 126
 
Name Accession Description Interval E-value
PIN_VapC4-5_FitB-like cd09881
VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae ...
 7-128 8.93e-31

VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae FitB and related proteins; This family includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This family belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350229  Cd Length: 127  Bit Score: 106.80  E-value: 8.93e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   7 CLDTSVLLDFFRKKDRDKT-FLNQLLEEfdEIEISSITEFEIRYGIN-----ENQSQFWDQLFENVNVLPFDSESAKKAV 80
Cdd:cd09881     1 LLDTNICIDLLRGRPPSVLeRLEELLPE--DLAISSITLAELLYGAEksqrpEKNLAALEAFLSRLEVLPFDEEAAEIYG 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1131868762  81 EIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLELL 128
Cdd:cd09881    79 EIRAELEKKGKPIGDNDLLIAATALAHGLTLVTNNTKHFERIPGLKVE 126
VapC COG1487
Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];
6-128 3.61e-29

Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];


Pssm-ID: 441096  Cd Length: 128  Bit Score: 102.63  E-value: 3.61e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   6 ICLDTSVLLDFFRKKDRD-KTFLNQLleEFDEIEISSITEFEIRYGI----NENQSQFWDQLFENVNVLPFDSESAKKAV 80
Cdd:COG1487     1 YLLDTNVLIDLLRGRPPAvLAWLAEL--EAGELAISAITLAELLYGArrspDAKRRAALEAFLEAFPVLPFDAEAARRAG 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1131868762  81 EIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLELL 128
Cdd:COG1487    79 ELRAALRRAGRPIGLADLLIAATALAHGLTLVTRNTKDFERIPGLKVE 126
PIN pfam01850
PIN domain;
6-125 3.77e-13

PIN domain;


Pssm-ID: 426475  Cd Length: 121  Bit Score: 61.66  E-value: 3.77e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   6 ICLDTSVLLDFFRKKDRDKTFLnQLLEEFDEIEISSITEFEIRYGIN-------ENQSQFWDQLFENVNVLPFDSESAKK 78
Cdd:pfam01850   1 IVLDTSVLIALLRGEPLHEAAR-ELLEAAGELVTSAIVLAELLYGLNrrlglgkAAELVELLLLLSALEVLPIDAELAEE 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1131868762  79 AVEIHRLLKKINkqiaIPDLFIASTAMRFKRPFASINKKhFERISGL 125
Cdd:pfam01850  80 AAELRLKYGKLG----PNDALIAATAKEHGAKLITFDED-FARVAKL 121
PRK13725 PRK13725
tRNA(fMet)-specific endonuclease VapC;
8-127 3.67e-03

tRNA(fMet)-specific endonuclease VapC;


Pssm-ID: 184277  Cd Length: 132  Bit Score: 35.39  E-value: 3.67e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKD---RDKTFLNQlleefDEIEISSITEFEIRYGINENQS-----QFWDQLFENVNVLPFDSESAKKA 79
Cdd:PRK13725    6 LDTNICIFTIKNKPehvRERFNLNT-----GRMCISSVTLMELIYGAEKSQMpernlAVIEGFVSRLEVLDYDTAAATHT 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1131868762  80 VEIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLEL 127
Cdd:PRK13725   81 GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVPGIRI 128
 
Name Accession Description Interval E-value
PIN_VapC4-5_FitB-like cd09881
VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae ...
 7-128 8.93e-31

VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae FitB and related proteins; This family includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This family belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350229  Cd Length: 127  Bit Score: 106.80  E-value: 8.93e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   7 CLDTSVLLDFFRKKDRDKT-FLNQLLEEfdEIEISSITEFEIRYGIN-----ENQSQFWDQLFENVNVLPFDSESAKKAV 80
Cdd:cd09881     1 LLDTNICIDLLRGRPPSVLeRLEELLPE--DLAISSITLAELLYGAEksqrpEKNLAALEAFLSRLEVLPFDEEAAEIYG 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1131868762  81 EIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLELL 128
Cdd:cd09881    79 EIRAELEKKGKPIGDNDLLIAATALAHGLTLVTNNTKHFERIPGLKVE 126
VapC COG1487
Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];
6-128 3.61e-29

Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];


Pssm-ID: 441096  Cd Length: 128  Bit Score: 102.63  E-value: 3.61e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   6 ICLDTSVLLDFFRKKDRD-KTFLNQLleEFDEIEISSITEFEIRYGI----NENQSQFWDQLFENVNVLPFDSESAKKAV 80
Cdd:COG1487     1 YLLDTNVLIDLLRGRPPAvLAWLAEL--EAGELAISAITLAELLYGArrspDAKRRAALEAFLEAFPVLPFDAEAARRAG 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1131868762  81 EIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLELL 128
Cdd:COG1487    79 ELRAALRRAGRPIGLADLLIAATALAHGLTLVTRNTKDFERIPGLKVE 126
PIN_VapC4-5_FitB-like cd18741
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-128 5.98e-25

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350308  Cd Length: 120  Bit Score: 91.79  E-value: 5.98e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKDRDKTFLNQLLEEfdEIEISSITEFEIRYGI-NENQSQFWDQLFENVNVLPFDSESAKKAVEIHRLL 86
Cdd:cd18741     2 LDTDILIDFLRGNPSAAEKLLELLGG--NLAISVITVAELYAGArNKKELEKLEKLLSLFEVIPVDEEIAELAGELRRKY 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1131868762  87 KKiNKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLELL 128
Cdd:cd18741    80 RK-SHGLGLPDALIAATALEHGLTLVTLNVKHFPMIEGLKLP 120
PIN_VapC4-5_FitB-like cd18754
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-126 1.38e-22

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350321  Cd Length: 128  Bit Score: 85.73  E-value: 1.38e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKDRDKTFLNQLLEEFDEIEISSITEFEIRYGI-----NENQSQFWDQLFENVNVLPFDSESAKKAVEI 82
Cdd:cd18754     2 LDTSFLIDLMRGDEAAIEKARELESESVPQRVSSPTVFELYVGVglsaaPEEERERIESVLESKPILPLDGESARKAGRI 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1131868762  83 HRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLE 126
Cdd:cd18754    82 DGQLRREGEPIDPGDAMIAATALVHDEPVLTRNVKHFERIDGLE 125
PIN_VapC4-5_FitB-like cd18750
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-127 1.10e-21

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350317  Cd Length: 127  Bit Score: 83.44  E-value: 1.10e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKkdrDKTFLNQL-LEEFDEIEISSITEFEIRYGINENQSQF------WDQLFENVNVLPFDSESAKKAV 80
Cdd:cd18750     2 LDTCTISDFVRG---NPNTLERLkSTSPSLIAISSITVMEIEYGLRLNPQRAkklrpiLEAFLSSITILPFDEEDAIAAA 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1131868762  81 EIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLEL 127
Cdd:cd18750    79 AIRAALKKQGTPIGAYDVLIAGTALAHGLILVTSNTKEFQRVDGLVI 125
PIN_VapC4-5_FitB-like cd18744
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 7-127 4.70e-21

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350311  Cd Length: 128  Bit Score: 81.77  E-value: 4.70e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   7 CLDTSVLLDFFRKKDRDKTFLNQLLEEFDEIEISSITEFEIRYGI----NENQSQFWDQLFENVNVLPFDSESAKKAVEI 82
Cdd:cd18744     1 LLDTDILSALLKGNPKVVEKFREYLKEYGKITISIITYYEILRGLlaknATKQLEVFEEFCSNNEVLPLDDESADIAAEI 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1131868762  83 HRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLEL 127
Cdd:cd18744    81 YADLRKKGTLIDDADILIAATAIANNLVLVTNNTKHFQRIEGLKL 125
PIN_VapC4-5_FitB-like cd18745
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 6-127 8.84e-20

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350312  Cd Length: 130  Bit Score: 78.69  E-value: 8.84e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   6 ICLDTSVLLDFFRKkdRDKTFLNQLLEE-FDEIEISSITEFEIRYGIN------ENQSQFwDQLFENVNVLPFDSESAKK 78
Cdd:cd18745     1 YLLDTNTVIALFRG--RGPVLRARLRAAaPSDLALSSIVLYELEYGAAksarpeRNRARL-EELLSLIEVLPFDEEDARA 77

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1131868762  79 AVEIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLEL 127
Cdd:cd18745    78 AAEIRADLERAGTPIGPYDTLIAGQALARGATLVTHNVREFSRVPGLKV 126
PIN_RfVapC1-like cd18733
VapC-like PIN domain of Rickettsia felis VapC1 and related proteins; This subfamily includes ...
 8-128 2.04e-15

VapC-like PIN domain of Rickettsia felis VapC1 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Rickettsia felis VapC1, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350300  Cd Length: 131  Bit Score: 67.63  E-value: 2.04e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFfrkkDRDKTFLNQLLEE--FDEIEISSITEFEIRYGI----NENQ----SQFWDQLFENVNVLPFDSESAK 77
Cdd:cd18733     4 IDTSVFIAA----ERGRLDLEALQQErpDEDVYISAITASELLHGVhrakTPERrarrEAFVEAILEAFPVLDFDLEVAR 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1131868762  78 KAVEIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLELL 128
Cdd:cd18733    80 THARLWAELAKRGITIGAHDLLIAATALAHGYSVLTANVRDFKRIPGLEVE 130
PIN_VapC4-5_FitB-like cd18751
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-125 1.98e-13

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350318  Cd Length: 133  Bit Score: 62.37  E-value: 1.98e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKD-----RDKTFLNQlleeFDEIEISSITEFEIRYGINENQ-----SQFWDQLfENVNVLPFDSESAK 77
Cdd:cd18751     2 LDTDILSEILKGKNpqvvaRARAYLAA----FGRYTISVITVMEVVRGLQKVQraerlQQFLEMI-ESAEVLPLDRTAAE 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1131868762  78 KAVEIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGL 125
Cdd:cd18751    77 LAGRIYADLERTGQPIGRADPMIAAIALRHGLVLVTGNTAHYQRIQEL 124
PIN_VapC4-5_FitB-like cd18752
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-127 2.10e-13

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350319  Cd Length: 130  Bit Score: 62.26  E-value: 2.10e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFrKKDRDKTFLNQLLEEFDEIE-ISSITEFEIRYG---INENQSQFWDQL---FENVNVLPFDSESAKKAV 80
Cdd:cd18752     2 FDTDAISELL-KKRPSPPLLERLNSIPREDQfTSAITVGELYYGayrSDRRLRLLEELLepvISRVTILPFDRESARVYG 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1131868762  81 EIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLEL 127
Cdd:cd18752    81 RLRAELEKKGTPLGEADLQIAAIALAHNLTLVTGNIRHFERIPGLRV 127
PIN pfam01850
PIN domain;
6-125 3.77e-13

PIN domain;


Pssm-ID: 426475  Cd Length: 121  Bit Score: 61.66  E-value: 3.77e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   6 ICLDTSVLLDFFRKKDRDKTFLnQLLEEFDEIEISSITEFEIRYGIN-------ENQSQFWDQLFENVNVLPFDSESAKK 78
Cdd:pfam01850   1 IVLDTSVLIALLRGEPLHEAAR-ELLEAAGELVTSAIVLAELLYGLNrrlglgkAAELVELLLLLSALEVLPIDAELAEE 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1131868762  79 AVEIHRLLKKINkqiaIPDLFIASTAMRFKRPFASINKKhFERISGL 125
Cdd:pfam01850  80 AAELRLKYGKLG----PNDALIAATAKEHGAKLITFDED-FARVAKL 121
PIN_MtVapC3-like_start cd09882
VapC-like PIN domain of Mycobacterium tuberculosis VapC3 toxin and related proteins; The ...
 9-127 8.38e-13

VapC-like PIN domain of Mycobacterium tuberculosis VapC3 toxin and related proteins; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, and VapC21. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350230  Cd Length: 128  Bit Score: 60.68  E-value: 8.38e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   9 DTSVLLDFFRKKDRD-KTFLNQLLEEFDEIEISSITEFEIRYGI-NENQSQFWDQLFENVNVLPFDSESAKKAVEIHRLL 86
Cdd:cd09882     3 DTSVWIDFFRGRDTPqVERLKELIEEGEDVALTGIILTEVLQGIrDEKEFQRVKEYLLALPILPLTGETYIAAARLYRRL 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1131868762  87 KKINKQIA-IPDLFIASTAMRFKRPFASiNKKHFERI---SGLEL 127
Cdd:cd09882    83 RRKGVTVRsTIDCLIAATAIENGLTLLH-NDRDFEAIakhTGLKL 126
PIN_MtVapC3-like cd18759
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 9-129 1.40e-12

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350326  Cd Length: 126  Bit Score: 59.91  E-value: 1.40e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   9 DTSVLLDFFRKKDRD-KTFLNQLLEEfDEIEISSITEFEIRYG-INENQSQFWDQLFENVNVLPFDSESAKKAVEIHRLL 86
Cdd:cd18759     3 DTSVWIEFFRGKSSPvSEVVDELLDE-DEVCTCGIVLAELLQGaKSEKELSLLEDFLSALPYLPITDELWIKAGELSFSL 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 1131868762  87 KKINKQIAIPDLFIASTAMRFKRPFASINkKHFERI---SGLELLF 129
Cdd:cd18759    82 RKKGITIPLSDLYIAALAIENNLELFTLD-KHFEIIpkhTGLKLYG 126
PIN_VapC4-5_FitB-like cd18738
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-128 1.33e-11

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350305  Cd Length: 118  Bit Score: 57.16  E-value: 1.33e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFrkKDRDKTFLNQLLEEFDEIEISSITEFEI---RYGINENQSQFWDqLFENVNVLPFDSESAKKAVEIHR 84
Cdd:cd18738     2 LDTNIIIYYL--KGELPESLEELLEELNEPAISVITKIELlgwPKLTPEEEEEIED-FLSLAEVIPLDDEIARKTIELRK 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1131868762  85 LLKkinkqIAIPDLFIASTAMRFKRPFASINKKHFERISGLELL 128
Cdd:cd18738    79 QYK-----IKLPDAIIAATALVHGLTLVTRNVKDFKKIPGLKLL 117
PIN_VapC4-5_FitB-like cd18748
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-125 2.22e-11

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350315  Cd Length: 128  Bit Score: 57.16  E-value: 2.22e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKdRDKTFLNQLLEEFDEIEISSITEFEIRYGINENQSQFWDQLFENV----NVLPFDSESAKKAVEIH 83
Cdd:cd18748     2 LDTNIISDLIRNP-RGKVARRIREVGEDAVCTSIIVASELRFGAAKKGSERLARRVEAVlsriPVLPLEPPADEHYARIR 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1131868762  84 RLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGL 125
Cdd:cd18748    81 ADLERQGTPIGPNDLLIAAHALALDLTLVTDNTREFSRVPGL 122
PIN_VapC4-5_FitB-like cd18753
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 7-127 2.39e-11

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350320  Cd Length: 123  Bit Score: 56.71  E-value: 2.39e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   7 CLDTSVLLDFFRKkdrDKTFLnQLLEEFDEIEISSITEFEIRYGIN------ENQSQFwDQLFEN--VNVLPFDSESAKK 78
Cdd:cd18753     1 LLDTNAYSALLRG---DPAVL-EALARADEIYLPVIVLGELLFGFRkgsreeENRERL-EEFLSSprVEVLPIDEETAEI 75

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1131868762  79 AVEIHRLLKKINKQIAIPDLFIASTAMRFKRPFASiNKKHFERISGLEL 127
Cdd:cd18753    76 YAEIKSALKKKGTPIPENDIWIAALALEHGLPLVT-RDAHFDKIPGLKL 123
PIN_NgFitB-like cd18731
VapC-like PIN domain of Neisseria gonorrhoeae FitB and related proteins; This subfamily ...
 6-127 4.41e-11

VapC-like PIN domain of Neisseria gonorrhoeae FitB and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Neisseria gonorrhoeae FitB toxin of the FitAB toxin/antitoxin (TA) system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. In N. gonorrhoeae, FitA and FitB form a heterodimer: FitA is the DNA binding subunit and FitB contains a ribonuclease activity that is blocked by the presence of FitA. A tetramer of FitAB heterodimers binds DNA from the fitAB upstream promoter region with high affinity. This results in both sequestration of FitAB and repression of fitAB transcription. It is thought that FitAB release from the DNA and subsequent dissociation both slows N. gonorrhoeae replication and transcytosis by an as yet undefined mechanism. The toxin M. tuberculosis VapC is a structural homolog of N. gonorrhoeae FitB, but their antitoxin partners, VapB and FitA, respectively, differ structurally. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350298  Cd Length: 136  Bit Score: 56.27  E-value: 4.41e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   6 ICLDTSVLLDFFRKK--DRDKTFLNQLLEEfdEIEISSITEFEIRYGIN-----ENQSQF---WDQLFENV---NVLPFD 72
Cdd:cd18731     1 IVLDTNVVSELMRPApdPAVLAWLDAQPAE--DLYLTAITVAELLYGIAllpdgRRRDALeaaAEALLAEDfagRILPFD 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1131868762  73 SESAKKAVEIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERIsGLEL 127
Cdd:cd18731    79 AAAAEAYAEIVAARRRAGRPISVADAQIAAIARAHGATLATRNVKDFEGL-GLEL 132
PIN_VapC4-5_FitB-like cd18739
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 7-126 6.21e-11

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350306  Cd Length: 124  Bit Score: 55.80  E-value: 6.21e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   7 CLDTSVLLDFFRKKDRDKTFLNQllEEFDEIEISSITEFEIRYGI-----NENQSQFwDQLFENVNVLPFDSESAKKAVE 81
Cdd:cd18739     1 CLDSSFLIDYLDGVDAAQAFLES--QEDEPLVTPTIALYEVYVGAvrsagETDLDGV-REDLDWVEPLDFDEEVAREAAQ 77

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1131868762  82 IHRLLKKINKQIAIPDLFIASTAMRFKRPFASiNKKHFERISGLE 126
Cdd:cd18739    78 IQAELLEAGEPINPPDLMIAATARSLGATLVT-RDGDFERIDGLE 121
VapC COG1848
VapC family ribonuclease, toxin component of the VapBC toxin-antitoxin module, contains PIN ...
 6-129 1.75e-10

VapC family ribonuclease, toxin component of the VapBC toxin-antitoxin module, contains PIN domain [Defense mechanisms];


Pssm-ID: 441453  Cd Length: 134  Bit Score: 54.99  E-value: 1.75e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   6 ICLDTSVLLDFFRKKDRD----KTFLNQLLEEFDEIEISSITEFEIRY------GINENQS-QFWDQLFENVNVLPFDSE 74
Cdd:COG1848     2 ILLDTNVLIYALEGDSPFheraRELLERAEEGEEELYTSPLVLAEVLYvltrpgGLSLEEArELLEALLSNIEVVPVDEE 81

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1131868762  75 SAKKAVEIHRllkkiNKQIAIPDLFIASTAMRFK-RPFASINkKHFERISGLELLF 129
Cdd:COG1848    82 ILEEAAELLA-----KYGLRLNDALHLATALEHGaDALVTFD-RDFARVPGLEVVD 131
PIN_RfVapC2-like cd18734
VapC-like PIN domain of Rickettsia felis VapC2 and related proteins; This subfamily includes ...
 8-127 3.57e-10

VapC-like PIN domain of Rickettsia felis VapC2 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Rickettsia felis VapC2, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. Rickettsia felis VapC2 cleaves single-stranded RNA. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350301  Cd Length: 129  Bit Score: 54.04  E-value: 3.57e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKDRDKTFLNQLLEEFDEIEISSITEFEIRYGINenQSQFWDQLFENVN-------VLPFDSESAKKAV 80
Cdd:cd18734     2 LDTNICVYLIKKKPDSYYARLEKLQKNNDIAISSIVLAELQYGVS--KSQYKEQNQAALNmflmkleVLPFEEKATFYYG 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1131868762  81 EIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLEL 127
Cdd:cd18734    80 EIRAELEKSGQVIGGNDLLIAAHALAENATLVTNNIKEFARVPNLIL 126
PIN_VapC4-5_FitB-like cd18742
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 35-127 4.63e-10

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350309  Cd Length: 127  Bit Score: 53.70  E-value: 4.63e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762  35 DEIEISSITEFEIRYG------INENQSQFwDQLFENVNVLPFDSESAKKAVEIHRLLKKINKQIAIPDLFIASTAMRFK 108
Cdd:cd18742    27 EPLSISVITYAELYYGaynsqrVEKNLARV-ERFKELLPVLPLSEAIAETFGKIKADLRKKGNPIDDFDLLIASTALANN 105

                          90
                  ....*....|....*....
gi 1131868762 109 RPFASINKKHFERISGLEL 127
Cdd:cd18742   106 LILVTNNTKHFKRIPGLKI 124
PIN_VapC4-5_FitB-like cd18743
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-127 9.52e-10

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350310  Cd Length: 127  Bit Score: 52.52  E-value: 9.52e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKdrdktflNQLLEEFDEIE-----ISSITEFEIRYG------INENQSQfWDQLFENVNVLPFdSESA 76
Cdd:cd18743     2 LDTNICIFLLRGK-------YGVAEKIEEVGlencfISEITIAELLYGaensarPEKNRKL-VDDFLSLFTVLPI-SDAL 72

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1131868762  77 KKAVEIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLEL 127
Cdd:cd18743    73 EEYAKEKARLRRQGKLIDDFDLLIGATAIANNLTLVTENVKHFARIPGIKL 123
PIN_MtVapC3-like cd18763
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 9-123 1.72e-08

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350330  Cd Length: 128  Bit Score: 49.58  E-value: 1.72e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   9 DTSVLLDFFRKKDRDKT-FLNQLLEEFDEIEISSITEFEIRYGI-NENQSQFWDQLFENVNVLPFDSESAKKAVEIHRLL 86
Cdd:cd18763     3 DTSVWIDFFRGRDTPQVaLLEELIEQREDLCICGVILTEVLQGIrDDKEYAKTKELLESLLFLPMTQSTFIRAADIYRTL 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1131868762  87 KKinKQIAIP---DLFIASTAMRFKRPFASiNKKHFERIS 123
Cdd:cd18763    83 RK--KGITIRkpvDCMIAAVALEHDVSLLH-NDRDFDPIA 119
PIN_VapC4-5_FitB-like cd18747
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-127 1.90e-08

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350314  Cd Length: 132  Bit Score: 49.46  E-value: 1.90e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFrKKDRDKTFLNQLLEEFDEIEISSITEFEIRYGIN--------ENQSQFWDQLFENVNVLPFDSESAKKA 79
Cdd:cd18747     2 LDTNILSEPL-KPEPNANVLARLERHEGEIAIAAVVWHELLYGCYrlpegkrkRILEYYLEDVVSGLPILPYDEAAAEWH 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1131868762  80 VEIHRLLKKINKQIAIPDLFIASTAM--------RfkrpfasiNKKHFERISGLEL 127
Cdd:cd18747    81 AEERARLTAIGKTPPFADGQIAAIAVannlilvtR--------NTKDFQGFEGLRL 128
PIN_VapC-FitB-like cd09875
VapC-like PIN domain of ribonucleases (toxins), VapC and FitB, of prokaryotic toxin/antitoxin ...
 8-128 2.09e-08

VapC-like PIN domain of ribonucleases (toxins), VapC and FitB, of prokaryotic toxin/antitoxin operons, Pyrococcus horikoshii protein PH0500, and other similar bacterial and archaeal homologs; PIN (PilT N terminus) domain-containing proteins of prokaryotic toxin/antitoxin (TA) operons, such as, Mycobacterium tuberculosis VapC of the VapBC (virulence associated proteins) TA operon, and Neisseria gonorrhoeae FitB of the FitAB (fast intracellular trafficking) TA operon, as well as, the archaeal Pyrococcus horikoshii protein PH0500 are included in this family. Toxins of TA operons are believed to be involved in growth inhibition by regulating translation and are nearly always co-expressed with an antitoxin, a cognate protein inhibitor, forming an inert protein complex. Disassociation of the complex activates the ribonuclease activity of the toxin. In N. gonorrhoeae, FitA and FitB form a heterodimer: FitA is the DNA binding subunit and FitB contains a ribonuclease activity that is blocked by the presence of FitA. A tetramer of FitAB heterodimers binds DNA from the fitAB upstream promoter region with high affinity. This results in both sequestration of FitAB and repression of fitAB transcription. It is thought that FitAB release from the DNA and subsequent dissociation both slows N. gonorrhoeae replication and transcytosis by an as yet undefined mechanism. The toxin M. tuberculosis VapC is a structural homolog of N. gonorrhoeae FitB, but their antitoxin partners, VapB and FitA, respectively, differ structurally. The M. tuberculosis VapC-5 is proposed to be both an endoribonuclease and an exoribonuclease that can act on free RNA in a similar manner to the endo and exonuclease Flap endonuclease-1 (FEN1). VapC-like toxins are structural homologs of FEN1-like PIN domains, but lack the extensive arch/clamp region and the H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region, seen in FEN1-like PIN domains. PIN domains within this group typically contain three or four conserved acidic residues that cluster at the C-terminal end of the beta-sheet and form a negatively charged pocket near the center of the molecule. These putative active site residues are thought to bind Mg2+ and/or Mn2+ ions and be essential for single-stranded ribonuclease activity. VapC-like PIN domains are single domain proteins that form dimers and dimerization configures the active sites in a groove along the long-axis of the structure.


Pssm-ID: 350223  Cd Length: 130  Bit Score: 49.49  E-value: 2.09e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFR--KKDRDKTFLNQLLEEfdEIEISSITEFEIRYGINENQSQFWDQLFENVN--------VLPFDSESAK 77
Cdd:cd09875     2 LDTSVLIWLLDndPKSHLRSLADRIERG--KVYVSIVSRWELAIGVNINKLRLNYAFADLENlladlaipVLPLTPRIAD 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1131868762  78 KAVEIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKhFERISGLELL 128
Cdd:cd09875    80 EYLEACRNLPLRGRHRSPFDRMIAAQALQHGFTVAHRDED-FKAINAVNLE 129
PIN_CcVapC1-like cd18736
VapC-like PIN domain of Caulobacter Crescentus VapC1-like and related proteins; This subfamily ...
 8-125 2.59e-08

VapC-like PIN domain of Caulobacter Crescentus VapC1-like and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Caulobacter Crescentus VapC1, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350303  Cd Length: 123  Bit Score: 48.72  E-value: 2.59e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKDRDktfLNQLLEEFD--EIEISSITEFEIRYGIN---ENQSQFWDQLFENVNVLPFDSESAKKAVEI 82
Cdd:cd18736     2 LDTNICIYLLKGQPPA---LARRVAACEpgEVVISAITLAELAYGVRrsaPPRRAALDALLEVIPVLPFDEAAAAAYARI 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 1131868762  83 HRLLKKINKQIaipDLFIASTAMRFKRPFASINKKHFERISGL 125
Cdd:cd18736    79 RAAFPFRRASF---DRLIAAHALALGLTLVTNNEADFADIPGL 118
PIN_VapC4-5_FitB-like cd18740
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-127 1.24e-07

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350307  Cd Length: 126  Bit Score: 47.17  E-value: 1.24e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKkdRDKTFLNQLLEE-FDEIEISSITEFEIRYGI-----NENQSQFWDQLFENVNVLPFDSESAKKAVE 81
Cdd:cd18740     2 LDTNTVSHIIKG--RPPAVRERLRAVpMASICISSITEAELLYGLakrpeAKRLHAAVREFLLRVDVLPWDSSVARTYGD 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1131868762  82 IHRLLKKINKQIAIPDLFIASTAMrfkrpfaSI------NKKHFERISGLEL 127
Cdd:cd18740    80 LRAALEAQGKTLGPLDMMIAAHAL-------ALgatlvtNDRAFRRVPGLAV 124
PIN_VapC4-5_FitB-like cd18749
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-128 2.27e-07

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350316  Cd Length: 126  Bit Score: 46.47  E-value: 2.27e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKDRDKTFLNQLleEFDEIEISSITEFEIRYGINENQsqfW-----DQLFE---NVNVLPFDSESAKKA 79
Cdd:cd18749     2 LDTDVASYLFKGRLLAALYAPRL--VGKILVISFVTVAELYFWAEKRG---WgekrlQQLEAwlsRYVVLPYDEEIARTW 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1131868762  80 VEIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLELL 128
Cdd:cd18749    77 GRLRAEAQRRGRPRPTNDAWIAATALTHGLPLATHNVKDFEDFPGLQLI 125
PIN_VapC4-5_FitB-like cd18746
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-128 3.70e-07

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350313 [Multi-domain]  Cd Length: 133  Bit Score: 45.89  E-value: 3.70e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFfRKKDRDK---TFLNQLLEefDEIEISSITEFEIRYGI------NENQ----SQFWDQL---FENvNVLPF 71
Cdd:cd18746     2 LDTNVISEL-RKPRPDPgvvAWLAAVDE--DDLFLSVITIGEIRKGIerlrrrDPARaarlEAWLDGLllrFAG-RILPV 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1131868762  72 DSESAKKAVEIHRLLKkinKQIAIPDLFIASTAM--------RfkrpfasiNKKHFERiSGLELL 128
Cdd:cd18746    78 DAEVARRWGRLHAPAP---APLPVADALIAATALvhgltlvtR--------NVRDFEG-TGVPLL 130
PIN_VapC4-5_FitB-like cd18737
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 8-119 3.51e-06

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350304  Cd Length: 115  Bit Score: 42.91  E-value: 3.51e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKDRDKtflnQLLEEFDEIEISSITEFEIRYGINENQSQFWDQLFENVNVLPFDSESAKKAVEIHRLLK 87
Cdd:cd18737     2 FDTNILIDYLNGVPQAR----AELERYEQPAISVITWMEVLVGAPPEEEEATRAFLSSFEIIPLDQAIAERAVALRRQSR 77

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1131868762  88 kinkqIAIPDLFIASTAMRFKRPFASINKKHF 119
Cdd:cd18737    78 -----LKLPDAIILATAQVHGLILVTRNTKDF 104
PIN_MtVapC3-like cd18764
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 9-123 5.22e-06

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350331  Cd Length: 129  Bit Score: 42.95  E-value: 5.22e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   9 DTSVLLDFFRKKDRDKT-FLNQLLEEFDEIEISSITEFEIRYGIN-ENQSQFWDQLFENVNVLPFDS-ESAKKAVEIHRL 85
Cdd:cd18764     3 DTSVWIDFFNGKETPQTdLLQELLLEDQPVYICPTIVQEILQGIRdDNEFKKIKDLLSALPILELDPyEAAIGAADLYRS 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1131868762  86 LKKinKQIAI---PDLFIASTAMRFKRPFASiNKKHFERIS 123
Cdd:cd18764    83 LRK--KGVTIrksNDCLIAHYAIKFDLPLVH-NDRDFDLIA 120
PIN_HiVapC1-like cd18735
VapC-like PIN domain of Haemophilus influenzae VapC1 and related proteins; Haemophilus ...
 8-127 1.42e-05

VapC-like PIN domain of Haemophilus influenzae VapC1 and related proteins; Haemophilus influenzae VapC1 has endonucleolytic activity with RNA, it cleaves initiator tRNA between the anticodon stem and loop, but does not cleave mRNA, rRNA or tmRNA, and has no activity on ssDNA or dsDNA. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350302  Cd Length: 128  Bit Score: 41.82  E-value: 1.42e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLdFFRKKdRDKTFLNQLLE-EFDEIEISSITEFEIRYGINENQS-----QFWDQLFENVNVLPFDSESAKKAVE 81
Cdd:cd18735     2 LDTNICI-YIRKQ-RPPSVLERFAQlQPGEVAMSVITYGELLYGAEKSQRrakalEALERLAELIPVLPLPEEVAEHYGE 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 1131868762  82 IHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLEL 127
Cdd:cd18735    80 IRADLEKKGTPIGNNDLWIAAHALALGLTLVTNNTREFQRVPGLKV 125
PIN_MtVapC3-like cd18758
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 9-123 2.05e-04

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350325 [Multi-domain]  Cd Length: 127  Bit Score: 38.34  E-value: 2.05e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   9 DTSVLLDFFRKK--DRDKTFLNQLLEEfDEIEISSITEFEIRYGI-NENQSQFWDQLFENVNVLPFDSESAKKAVEIHRL 85
Cdd:cd18758     3 DTSVWIEALRPRgsPKVKEKVREALSE-DRVATTGIIILELLRGAkTEKEYEELKEDLEALPYLPIEEEVWERAAKLGFD 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1131868762  86 LKKinKQIAIP--DLFIASTAMRFKRPFASINkKHFERIS 123
Cdd:cd18758    82 LRR--KGLRVPltDLLIAAVALKHNCELLHAD-RHFDLIA 118
PIN_VapC-like cd18689
uncharacterized subfamily of the VapC-like nuclease family of the PIN domain superfamily; VapC ...
 8-110 1.21e-03

uncharacterized subfamily of the VapC-like nuclease family of the PIN domain superfamily; VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350256  Cd Length: 125  Bit Score: 36.46  E-value: 1.21e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKDRD--KTFLNQLLEEFDEIEISSITEFEI------RYGINENQSQFwDQLFEN-VNVLPFDSESAKK 78
Cdd:cd18689     2 LDSGALLAYFEGEPHKvaKKLIELIKEGKAELYISAINLGEVyyitarKKGEEKAEEVL-SLLLALpIEVVEADEELVEA 80

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1131868762  79 AVEIhrllkKINKQIAIPDLFIASTAMRFKRP 110
Cdd:cd18689    81 AAKI-----KAKYGLSYADCFALATAKELNAA 107
PIN_MtVapC3-like cd18766
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 8-123 2.53e-03

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350333  Cd Length: 130  Bit Score: 35.48  E-value: 2.53e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKK----DRDKTFLNQLLEEFDEIEISSITEFEIRYGInENQSQFWD--QLFENVNVLPFDSESAKKAVE 81
Cdd:cd18766     2 VDTSVWSLALRRPkhavNREATLLKQIIEKGEPIALTGIILQELLQGI-RNDKLFDQlkEHLEAFPLILPTREDHIEAAR 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1131868762  82 IHRLLKKINKQIAIPDLFIASTAMRFKRPFASINkKHFERIS 123
Cdd:cd18766    81 LRNDCRRKGVQVSAIDALIAQMCISHDLTLFTTD-KDFKHIA 121
PRK13725 PRK13725
tRNA(fMet)-specific endonuclease VapC;
8-127 3.67e-03

tRNA(fMet)-specific endonuclease VapC;


Pssm-ID: 184277  Cd Length: 132  Bit Score: 35.39  E-value: 3.67e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1131868762   8 LDTSVLLDFFRKKD---RDKTFLNQlleefDEIEISSITEFEIRYGINENQS-----QFWDQLFENVNVLPFDSESAKKA 79
Cdd:PRK13725    6 LDTNICIFTIKNKPehvRERFNLNT-----GRMCISSVTLMELIYGAEKSQMpernlAVIEGFVSRLEVLDYDTAAATHT 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1131868762  80 VEIHRLLKKINKQIAIPDLFIASTAMRFKRPFASINKKHFERISGLEL 127
Cdd:PRK13725   81 GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVPGIRI 128
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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