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Conserved domains on  [gi|1170956475|emb|SLY40052|]
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UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase [Klebsiella quasivariicola]

Protein Classification

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase( domain architecture ID 11478177)

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

CATH:  3.40.1390.10|3.40.1190.10|3.90.190.20
EC:  6.3.2.13
Gene Ontology:  GO:0005524|GO:0008765|GO:0009252|GO:0000287
PubMed:  11124264|28201974
SCOP:  4000234

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
murE PRK00139
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional
 1-493 0e+00

UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional


:

Pssm-ID: 234660 [Multi-domain]  Cd Length: 460  Bit Score: 640.26  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475   1 MADRNLRDLLAPWVpnaperiLREMTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEdgeiremH 80
Cdd:PRK00139    1 MASMKLRDLLAPVE-------ITGLTYDSRKVKPGDLFVALPGHKVDGRDFIAQAIANGAAAVVAEADGEAG-------T 66

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  81 GVPVIFLSQLNERLSALAGRFYHQPSQQLRLVgvtgtngkttttQLLAQWAKLLGETSAVMGTVGNGLLDKVVPTENTTG 160
Cdd:PRK00139   67 GVPVIIVPDLRKALALLAAAFYGHPSDKLKLIgvtgtngktttaYLLAQILRLLGEKTALIGTLGNGIGGELIPSGLTTP 146

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 161 SAVDVQHVLSSLVEQGATFGAMEVSSHGLVQHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYStHHSGQAIVN 240
Cdd:PRK00139  147 DALDLQRLLAELVDAGVTYAAMEVSSHALDQGRVDGLKFDVAVFTNLSRDHLDYHGTMEDYLAAKARLFS-ELGLAAVIN 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 241 ADDEVGRRWLAKlPDAVAVSMedhinpncHGRWLKTTAVSYHDSGATIQFDsswgqGEIESRLMGAFNVSNLLLALATLL 320
Cdd:PRK00139  226 ADDEVGRRLLAL-PDAYAVSM--------AGADLRATDVEYTDSGQTFTLV-----TEVESPLIGRFNVSNLLAALAALL 291

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 321 ALGYPLADLLKTAARLQPVCGRMEVFSAPGKPAVVVDYAHTPDALEKALQAARLHCSGKLWCVFGCGGDRDKGKRPLMGA 400
Cdd:PRK00139  292 ALGVPLEDALAALAKLQGVPGRMERVDAGQGPLVIVDYAHTPDALEKVLEALRPHAKGRLICVFGCGGDRDKGKRPLMGA 371

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 401 IAEEFADIVVVTDDNPRTEEPRAIINDILAGMldaghAKVMEGRAEAVTNAVMQAKENDVVLVAGKGHEDYQIVGNRRLD 480
Cdd:PRK00139  372 IAERLADVVIVTSDNPRSEDPAAIIADILAGI-----YDVIEDRAEAIRYAIAQAKPGDVVLIAGKGHEDYQIIGGVKIP 446

                         490
                  ....*....|...
gi 1170956475 481 YSDRVTVARLLGA 493
Cdd:PRK00139  447 FDDREVAREALAE 459
 
Name Accession Description Interval E-value
murE PRK00139
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional
 1-493 0e+00

UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional


Pssm-ID: 234660 [Multi-domain]  Cd Length: 460  Bit Score: 640.26  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475   1 MADRNLRDLLAPWVpnaperiLREMTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEdgeiremH 80
Cdd:PRK00139    1 MASMKLRDLLAPVE-------ITGLTYDSRKVKPGDLFVALPGHKVDGRDFIAQAIANGAAAVVAEADGEAG-------T 66

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  81 GVPVIFLSQLNERLSALAGRFYHQPSQQLRLVgvtgtngkttttQLLAQWAKLLGETSAVMGTVGNGLLDKVVPTENTTG 160
Cdd:PRK00139   67 GVPVIIVPDLRKALALLAAAFYGHPSDKLKLIgvtgtngktttaYLLAQILRLLGEKTALIGTLGNGIGGELIPSGLTTP 146

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 161 SAVDVQHVLSSLVEQGATFGAMEVSSHGLVQHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYStHHSGQAIVN 240
Cdd:PRK00139  147 DALDLQRLLAELVDAGVTYAAMEVSSHALDQGRVDGLKFDVAVFTNLSRDHLDYHGTMEDYLAAKARLFS-ELGLAAVIN 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 241 ADDEVGRRWLAKlPDAVAVSMedhinpncHGRWLKTTAVSYHDSGATIQFDsswgqGEIESRLMGAFNVSNLLLALATLL 320
Cdd:PRK00139  226 ADDEVGRRLLAL-PDAYAVSM--------AGADLRATDVEYTDSGQTFTLV-----TEVESPLIGRFNVSNLLAALAALL 291

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 321 ALGYPLADLLKTAARLQPVCGRMEVFSAPGKPAVVVDYAHTPDALEKALQAARLHCSGKLWCVFGCGGDRDKGKRPLMGA 400
Cdd:PRK00139  292 ALGVPLEDALAALAKLQGVPGRMERVDAGQGPLVIVDYAHTPDALEKVLEALRPHAKGRLICVFGCGGDRDKGKRPLMGA 371

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 401 IAEEFADIVVVTDDNPRTEEPRAIINDILAGMldaghAKVMEGRAEAVTNAVMQAKENDVVLVAGKGHEDYQIVGNRRLD 480
Cdd:PRK00139  372 IAERLADVVIVTSDNPRSEDPAAIIADILAGI-----YDVIEDRAEAIRYAIAQAKPGDVVLIAGKGHEDYQIIGGVKIP 446

                         490
                  ....*....|...
gi 1170956475 481 YSDRVTVARLLGA 493
Cdd:PRK00139  447 FDDREVAREALAE 459
MurE COG0769
UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 25-493 0e+00

UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl tripeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440532 [Multi-domain]  Cd Length: 459  Bit Score: 605.15  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  25 MTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEDgeiremHGVPVIFLSQLNERLSALAGRFYHQ 104
Cdd:COG0769     2 ITYDSRKVKPGDLFVALPGARVDGHDFIAQAIARGAVAVVTEAPGALLA------AGVPVIVVPDPRAALALLAAAFYGH 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 105 PSQQLRLVgvtgtngkttttQLLAQWAKLLGETSAVMGTVGNGLLDKVVPTENTTGSAVDVQHVLSSLVEQGATFGAMEV 184
Cdd:COG0769    76 PSQKLKLIgvtgtngkttttYLLAQILRALGKKTGLIGTVGNGIGGELIPSSLTTPEALDLQRLLAEMVDAGVTHVVMEV 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 185 SSHGLVQHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYSTH-HSGQAIVNADDEVGRRWLAKLPDAV-AVSME 262
Cdd:COG0769   156 SSHALDQGRVDGVRFDVAVFTNLTRDHLDYHGTMEAYFAAKARLFDQLgPGGAAVINADDPYGRRLAAAAPARViTYGLK 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 263 DhinpnchGRWLKTTAVSYHDSGATIQFDSSWGQGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAARLQPVCGR 342
Cdd:COG0769   236 A-------DADLRATDIELSADGTRFTLVTPGGEVEVRLPLIGRFNVYNALAAIAAALALGIDLEEILAALEKLKGVPGR 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 343 MEVFSAPGKPAVVVDYAHTPDALEKALQAARLHCSGKLWCVFGCGGDRDKGKRPLMGAIAEEFADIVVVTDDNPRTEEPR 422
Cdd:COG0769   309 MERVDGGQGPTVIVDYAHTPDALENVLEALRPHTKGRLIVVFGCGGDRDRGKRPLMGEIAARLADVVIVTSDNPRSEDPA 388

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1170956475 423 AIINDILAGMLDAGHAKVMEGRAEAVTNAVMQAKENDVVLVAGKGHEDYQIVGNRRLDYSDRVTVARLLGA 493
Cdd:COG0769   389 AIIADILAGIPGAGKVLVIPDRAEAIRYAIALAKPGDVVLIAGKGHETYQIIGGVKIPFDDREVAREALAE 459
murE TIGR01085
UDP-N-acetylmuramyl-tripeptide synthetase; Most members of this family are EC 6.3.2.13, ...
 25-484 3.19e-171

UDP-N-acetylmuramyl-tripeptide synthetase; Most members of this family are EC 6.3.2.13, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase. An exception is Staphylococcus aureus, in which diaminopimelate is replaced by lysine in the peptidoglycan and MurE is EC 6.3.2.7. The Mycobacteria, part of the closest neighboring branch outside of the low-GC Gram-positive bacteria, use diaminopimelate. A close homolog, scoring just below the trusted cutoff, is found (with introns) in Arabidopsis thaliana. Its role is unknown. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273435 [Multi-domain]  Cd Length: 464  Bit Score: 490.29  E-value: 3.19e-171
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  25 MTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEdgeireMHGVPVIFLSQLNERLSALAGRFYHQ 104
Cdd:TIGR01085   7 LTLDSREVKPGDLFVAIKGTHVDGHDFIHDAIANGAVAVVVERDVDFY------VAPVPVIIVPDLRHALSSLAAAFYGH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 105 PSQQLRLVGVTGTNGKTTTTQLLAQWAKLLGETSAVMGTVGNGLLDKVV---PTENTTGSAVDVQHVLSSLVEQGATFGA 181
Cdd:TIGR01085  81 PSKKLKVIGVTGTNGKTTTTSLIAQLLRLLGKKTGLIGTIGYRLGGNDLiknPAALTTPEALTLQSTLAEMVEAGAQYAV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 182 MEVSSHGLVQHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYSTHH-SGQAIVNADDEVGRRWLAKLPDAVAVS 260
Cdd:TIGR01085 161 MEVSSHALAQGRVRGVRFDAAVFTNLSRDHLDFHGTMENYFAAKASLFTELGlKRFAVINLDDEYGAQFVKRLPKDITVS 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 261 MEDHINPNCHGrWLKTTAVSYHDSGATIQFDSSWGQGEIESRLMGAFNVSNLLLALATLLALG-YPLADLLKTAARLQPV 339
Cdd:TIGR01085 241 AITQPADGRAQ-DIKITDSGYSFEGQQFTFETPAGEGHLHTPLIGRFNVYNLLAALATLLHLGgIDLEDIVAALEKFRGV 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 340 CGRMEVFSAPGKPAVVVDYAHTPDALEKALQAARLHCSGKLWCVFGCGGDRDKGKRPLMGAIAEEFADIVVVTDDNPRTE 419
Cdd:TIGR01085 320 PGRMELVDGGQKFLVIVDYAHTPDALEKALRTLRKHKDGRLIVVFGCGGDRDRGKRPLMGAIAEQLADLVILTSDNPRGE 399

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1170956475 420 EPRAIINDILAGMLDAGHAKVMEGRAEAVTNAVMQAKENDVVLVAGKGHEDYQIVGNRRLDYSDR 484
Cdd:TIGR01085 400 DPEQIIADILAGISEKEKVVIIADRRQAIRYAISNAKAGDVVLIAGKGHEDYQIIGGETIPFDDR 464
Mur_ligase_M pfam08245
Mur ligase middle domain;
125-311 2.13e-50

Mur ligase middle domain;


Pssm-ID: 462409 [Multi-domain]  Cd Length: 199  Bit Score: 170.56  E-value: 2.13e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 125 QLLAQWAKLLGetsAVMGTVGNGLLdkvvPTENTTGSAVDVQHVLSSLVEQGATFGAMEVSSHGLVQHRVAAL-QFAASV 203
Cdd:pfam08245  12 ELIAAILSLAG---GVIGTIGTYIG----KSGNTTNNAIGLPLTLAEMVEAGAEYAVLEVSSHGLGEGRLSGLlKPDIAV 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 204 FTNLSRDHLDYHGDMEHYEAAKWLLYSTHHS-GQAIVNADDEVGRRWLAKL----PDAVAVSMEDHInpnchgrWLKTTA 278
Cdd:pfam08245  85 FTNISPDHLDFHGTMENYAKAKAELFEGLPEdGIAVINADDPYGAFLIAKLkkagVRVITYGIEGEA-------DLRAAN 157

                         170       180       190
                  ....*....|....*....|....*....|....
gi 1170956475 279 VSYHDSGATIQ-FDSSWGQGEIESRLMGAFNVSN 311
Cdd:pfam08245 158 IELSSDGTSFDlFTVPGGELEIEIPLLGRHNVYN 191
 
Name Accession Description Interval E-value
murE PRK00139
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional
 1-493 0e+00

UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional


Pssm-ID: 234660 [Multi-domain]  Cd Length: 460  Bit Score: 640.26  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475   1 MADRNLRDLLAPWVpnaperiLREMTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEdgeiremH 80
Cdd:PRK00139    1 MASMKLRDLLAPVE-------ITGLTYDSRKVKPGDLFVALPGHKVDGRDFIAQAIANGAAAVVAEADGEAG-------T 66

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  81 GVPVIFLSQLNERLSALAGRFYHQPSQQLRLVgvtgtngkttttQLLAQWAKLLGETSAVMGTVGNGLLDKVVPTENTTG 160
Cdd:PRK00139   67 GVPVIIVPDLRKALALLAAAFYGHPSDKLKLIgvtgtngktttaYLLAQILRLLGEKTALIGTLGNGIGGELIPSGLTTP 146

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 161 SAVDVQHVLSSLVEQGATFGAMEVSSHGLVQHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYStHHSGQAIVN 240
Cdd:PRK00139  147 DALDLQRLLAELVDAGVTYAAMEVSSHALDQGRVDGLKFDVAVFTNLSRDHLDYHGTMEDYLAAKARLFS-ELGLAAVIN 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 241 ADDEVGRRWLAKlPDAVAVSMedhinpncHGRWLKTTAVSYHDSGATIQFDsswgqGEIESRLMGAFNVSNLLLALATLL 320
Cdd:PRK00139  226 ADDEVGRRLLAL-PDAYAVSM--------AGADLRATDVEYTDSGQTFTLV-----TEVESPLIGRFNVSNLLAALAALL 291

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 321 ALGYPLADLLKTAARLQPVCGRMEVFSAPGKPAVVVDYAHTPDALEKALQAARLHCSGKLWCVFGCGGDRDKGKRPLMGA 400
Cdd:PRK00139  292 ALGVPLEDALAALAKLQGVPGRMERVDAGQGPLVIVDYAHTPDALEKVLEALRPHAKGRLICVFGCGGDRDKGKRPLMGA 371

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 401 IAEEFADIVVVTDDNPRTEEPRAIINDILAGMldaghAKVMEGRAEAVTNAVMQAKENDVVLVAGKGHEDYQIVGNRRLD 480
Cdd:PRK00139  372 IAERLADVVIVTSDNPRSEDPAAIIADILAGI-----YDVIEDRAEAIRYAIAQAKPGDVVLIAGKGHEDYQIIGGVKIP 446

                         490
                  ....*....|...
gi 1170956475 481 YSDRVTVARLLGA 493
Cdd:PRK00139  447 FDDREVAREALAE 459
MurE COG0769
UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 25-493 0e+00

UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl tripeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440532 [Multi-domain]  Cd Length: 459  Bit Score: 605.15  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  25 MTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEDgeiremHGVPVIFLSQLNERLSALAGRFYHQ 104
Cdd:COG0769     2 ITYDSRKVKPGDLFVALPGARVDGHDFIAQAIARGAVAVVTEAPGALLA------AGVPVIVVPDPRAALALLAAAFYGH 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 105 PSQQLRLVgvtgtngkttttQLLAQWAKLLGETSAVMGTVGNGLLDKVVPTENTTGSAVDVQHVLSSLVEQGATFGAMEV 184
Cdd:COG0769    76 PSQKLKLIgvtgtngkttttYLLAQILRALGKKTGLIGTVGNGIGGELIPSSLTTPEALDLQRLLAEMVDAGVTHVVMEV 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 185 SSHGLVQHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYSTH-HSGQAIVNADDEVGRRWLAKLPDAV-AVSME 262
Cdd:COG0769   156 SSHALDQGRVDGVRFDVAVFTNLTRDHLDYHGTMEAYFAAKARLFDQLgPGGAAVINADDPYGRRLAAAAPARViTYGLK 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 263 DhinpnchGRWLKTTAVSYHDSGATIQFDSSWGQGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAARLQPVCGR 342
Cdd:COG0769   236 A-------DADLRATDIELSADGTRFTLVTPGGEVEVRLPLIGRFNVYNALAAIAAALALGIDLEEILAALEKLKGVPGR 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 343 MEVFSAPGKPAVVVDYAHTPDALEKALQAARLHCSGKLWCVFGCGGDRDKGKRPLMGAIAEEFADIVVVTDDNPRTEEPR 422
Cdd:COG0769   309 MERVDGGQGPTVIVDYAHTPDALENVLEALRPHTKGRLIVVFGCGGDRDRGKRPLMGEIAARLADVVIVTSDNPRSEDPA 388

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1170956475 423 AIINDILAGMLDAGHAKVMEGRAEAVTNAVMQAKENDVVLVAGKGHEDYQIVGNRRLDYSDRVTVARLLGA 493
Cdd:COG0769   389 AIIADILAGIPGAGKVLVIPDRAEAIRYAIALAKPGDVVLIAGKGHETYQIIGGVKIPFDDREVAREALAE 459
murE TIGR01085
UDP-N-acetylmuramyl-tripeptide synthetase; Most members of this family are EC 6.3.2.13, ...
 25-484 3.19e-171

UDP-N-acetylmuramyl-tripeptide synthetase; Most members of this family are EC 6.3.2.13, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase. An exception is Staphylococcus aureus, in which diaminopimelate is replaced by lysine in the peptidoglycan and MurE is EC 6.3.2.7. The Mycobacteria, part of the closest neighboring branch outside of the low-GC Gram-positive bacteria, use diaminopimelate. A close homolog, scoring just below the trusted cutoff, is found (with introns) in Arabidopsis thaliana. Its role is unknown. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273435 [Multi-domain]  Cd Length: 464  Bit Score: 490.29  E-value: 3.19e-171
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  25 MTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEdgeireMHGVPVIFLSQLNERLSALAGRFYHQ 104
Cdd:TIGR01085   7 LTLDSREVKPGDLFVAIKGTHVDGHDFIHDAIANGAVAVVVERDVDFY------VAPVPVIIVPDLRHALSSLAAAFYGH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 105 PSQQLRLVGVTGTNGKTTTTQLLAQWAKLLGETSAVMGTVGNGLLDKVV---PTENTTGSAVDVQHVLSSLVEQGATFGA 181
Cdd:TIGR01085  81 PSKKLKVIGVTGTNGKTTTTSLIAQLLRLLGKKTGLIGTIGYRLGGNDLiknPAALTTPEALTLQSTLAEMVEAGAQYAV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 182 MEVSSHGLVQHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYSTHH-SGQAIVNADDEVGRRWLAKLPDAVAVS 260
Cdd:TIGR01085 161 MEVSSHALAQGRVRGVRFDAAVFTNLSRDHLDFHGTMENYFAAKASLFTELGlKRFAVINLDDEYGAQFVKRLPKDITVS 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 261 MEDHINPNCHGrWLKTTAVSYHDSGATIQFDSSWGQGEIESRLMGAFNVSNLLLALATLLALG-YPLADLLKTAARLQPV 339
Cdd:TIGR01085 241 AITQPADGRAQ-DIKITDSGYSFEGQQFTFETPAGEGHLHTPLIGRFNVYNLLAALATLLHLGgIDLEDIVAALEKFRGV 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 340 CGRMEVFSAPGKPAVVVDYAHTPDALEKALQAARLHCSGKLWCVFGCGGDRDKGKRPLMGAIAEEFADIVVVTDDNPRTE 419
Cdd:TIGR01085 320 PGRMELVDGGQKFLVIVDYAHTPDALEKALRTLRKHKDGRLIVVFGCGGDRDRGKRPLMGAIAEQLADLVILTSDNPRGE 399

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1170956475 420 EPRAIINDILAGMLDAGHAKVMEGRAEAVTNAVMQAKENDVVLVAGKGHEDYQIVGNRRLDYSDR 484
Cdd:TIGR01085 400 DPEQIIADILAGISEKEKVVIIADRRQAIRYAISNAKAGDVVLIAGKGHEDYQIIGGETIPFDDR 464
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 23-491 4.36e-143

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 434.52  E-value: 4.36e-143
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  23 REMTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEDGEIRemhgVPVIFLSQLNERLSALAGRFY 102
Cdd:PRK11929   30 ADLRLDSREVQPGDLFVACRGAASDGRAFIDQALARGAAAVLVEAEGEDQVAAAD----ALVLPVADLRKALGELAARWY 105

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 103 HQPSQQLRLVGVTGTNGKTTTTQLLAQWAKLLGETSAVMGTVGNGLLDKVVPTENTTGSAVDVQHVLSSLVEQGATFGAM 182
Cdd:PRK11929  106 GRPSEQLSLVAVTGTNGKTSCAQLLAQLLTRLGKPCGSIGTLGARLDGRLIPGSLTTPDAIILHRILARMRAAGADAVAM 185

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 183 EVSSHGLVQHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYST-HHSGQAIVNADDEVGRRWLAKLPDAVAVSM 261
Cdd:PRK11929  186 EASSHGLEQGRLDGLRIAVAGFTNLTRDHLDYHGTMQDYEEAKAALFSKlPGLGAAVINADDPAAARLLAALPRGLKVGY 265

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 262 EDHINP-NCHGRWLKTTavsYHDSGATIQFDSswGQGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAARLQPVC 340
Cdd:PRK11929  266 SPQNAGaDVQARDLRAT---AHGQVFTLATPD--GSYQLVTRLLGRFNVSNLLLVAAALKKLGLPLAQIARALAAVSPVP 340

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 341 GRME-VFSAPGK--PAVVVDYAHTPDALEKALQAARL--HC-SGKLWCVFGCGGDRDKGKRPLMGAIAEEFADIVVVTDD 414
Cdd:PRK11929  341 GRMErVGPTAGAqgPLVVVDYAHTPDALAKALTALRPvaQArNGRLVCVFGCGGDRDKGKRPEMGRIAAELADRVVVTSD 420

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1170956475 415 NPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTNAVMQAKENDVVLVAGKGHEDYQIVGNRRLDYSDRVTVARLL 491
Cdd:PRK11929  421 NPRSEAPEAIIDQILAGIPAGARVFVISDRAEAIRQAIWMAAPGDVILIAGKGHETYQEIGGRKLFFDDREWARRAL 497
PRK14022 PRK14022
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase;
2-481 6.54e-69

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase;


Pssm-ID: 237588 [Multi-domain]  Cd Length: 481  Bit Score: 228.00  E-value: 6.54e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475   2 ADRNLRDLLAP--WVPNAPERILREMTLDSRVAASGDLFIAVQGhqADGRRYIPQAIAQGVAAIIAEAQGEAedgeirem 79
Cdd:PRK14022   12 KDHNFREIIDQdhYHYNYSGVQFDDISYDSRTADEGTLFFAKGA--YFKHKFLQNAITQGLKLYVSEKDYEV-------- 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  80 hGVPVIFLSQLNERLSALAGRFYHQPSQQLRLVGVTGTNGKTTTTQLLAQWAKLLGETSAVMGTvgNGLLD--KVVPTEN 157
Cdd:PRK14022   82 -GIPQVIVPDIKKAMSLIAMEFYDNPQHKLKLLAFTGTKGKTTAAYFAYHILKQLHKPAMLSTM--NTTLDgeTFFKSAL 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 158 TTGSAVDVQHVLSSLVEQGATFGAMEVSSHGLVQHRVAALQFAASVFTNLSRDHLDY--HGDMEHYEAAKWLLYSthHSG 235
Cdd:PRK14022  159 TTPESLDLFKMMAEAVDNGMTHLIMEVSSQAYLVGRVYGLTFDVGVFLNITPDHIGPieHPTFEDYFYHKRLLME--NSK 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 236 QAIVNADDEvgrrWLAKLPDAVAVSMEDHINPNCHGRWLKTTAVSYHDSGATIqfdsswgqGEIESRLMGAFNVSNLLLA 315
Cdd:PRK14022  237 AVVVNSDMD----HFSELLEQVTPQEHDFYGIDSENQIMASNAFSFEATGKLA--------GTYDIQLIGKFNQENAMAA 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 316 LATLLALGYPLADLLKTAARlQPVCGRMEVFSAPGKPAVVVDYAHTPDALEKALQAARLHCSGKLWCVFGCGGDRDKGKR 395
Cdd:PRK14022  305 GLACLRLGASLEDIQKGIAQ-TPVPGRMEVLTQSNGAKVFIDYAHNGDSLNKLIDVVEEHQKGKLILLLGAAGNKGESRR 383

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 396 PLMGAIAEEFADI-VVVTDDNPRTEEPRAIINDILAGMldAGHAKVMEGRAEAVTNAVMQA-KENDVVLVAGKGHEDYQI 473
Cdd:PRK14022  384 PDFGRVANRHPYLqVILTADDPNNEDPKMITQEIASHI--THPVEIIDDRAEAIKHAMSITeGPGDAVIIAGKGADAYQI 461


                  ....*...
gi 1170956475 474 VGNRRLDY 481
Cdd:PRK14022  462 VPGHRDDY 469
Mur_ligase_M pfam08245
Mur ligase middle domain;
125-311 2.13e-50

Mur ligase middle domain;


Pssm-ID: 462409 [Multi-domain]  Cd Length: 199  Bit Score: 170.56  E-value: 2.13e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 125 QLLAQWAKLLGetsAVMGTVGNGLLdkvvPTENTTGSAVDVQHVLSSLVEQGATFGAMEVSSHGLVQHRVAAL-QFAASV 203
Cdd:pfam08245  12 ELIAAILSLAG---GVIGTIGTYIG----KSGNTTNNAIGLPLTLAEMVEAGAEYAVLEVSSHGLGEGRLSGLlKPDIAV 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 204 FTNLSRDHLDYHGDMEHYEAAKWLLYSTHHS-GQAIVNADDEVGRRWLAKL----PDAVAVSMEDHInpnchgrWLKTTA 278
Cdd:pfam08245  85 FTNISPDHLDFHGTMENYAKAKAELFEGLPEdGIAVINADDPYGAFLIAKLkkagVRVITYGIEGEA-------DLRAAN 157

                         170       180       190
                  ....*....|....*....|....*....|....
gi 1170956475 279 VSYHDSGATIQ-FDSSWGQGEIESRLMGAFNVSN 311
Cdd:pfam08245 158 IELSSDGTSFDlFTVPGGELEIEIPLLGRHNVYN 191
Mur_ligase_C pfam02875
Mur ligase family, glutamate ligase domain; This family contains a number of related ligase ...
 339-425 3.95e-32

Mur ligase family, glutamate ligase domain; This family contains a number of related ligase enzymes which have EC numbers 6.3.2.*. This family includes: MurC, MurD, MurE, MurF, Mpl and FolC. MurC, MurD, Mure and MurF catalyze consecutive steps in the synthesis of peptidoglycan. Peptidoglycan consists of a sheet of two sugar derivatives, with one of these N-acetylmuramic acid attaching to a small pentapeptide. The pentapeptide is is made of L-alanine, D-glutamic acid, Meso-diaminopimelic acid and D-alanyl alanine. The peptide moiety is synthesized by successively adding these amino acids to UDP-N-acetylmuramic acid. MurC transfers the L-alanine, MurD transfers the D-glutamate, MurE transfers the diaminopimelic acid, and MurF transfers the D-alanyl alanine. This family also includes Folylpolyglutamate synthase that transfers glutamate to folylpolyglutamate.


Pssm-ID: 460731 [Multi-domain]  Cd Length: 87  Bit Score: 117.83  E-value: 3.95e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 339 VCGRMEVFSAPGKPAVVVDYAHTPDALEKALQAARLHCSGKLWCVFGCGGDRDKGKRPLMGAIAEEFADIVVVTDDNPRT 418
Cdd:pfam02875   1 VPGRLEVVGENNGVLVIDDYAHNPDAMEAALRALRNLFPGRLILVFGGMGDRDAEFHALLGRLAAALADVVILTGDYPRA 80


                  ....*..
gi 1170956475 419 EEPRAII 425
Cdd:pfam02875  81 EDPGAII 87
MurC COG0773
UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall ...
 202-467 1.02e-20

UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440536 [Multi-domain]  Cd Length: 451  Bit Score: 94.36  E-value: 1.02e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 202 SVFTNLSRDHLDYHGDMEHYEAA-KWLLYSTHHSGQAIVNADDEVGRRWLAKLPDAV---AVSMEDHInpnchgrwlktT 277
Cdd:COG0773   175 AVVTNIEADHLDIYGDLEAIKEAfHEFARNVPFYGLLVLCADDPGLRELLPRCGRPVityGFSEDADY-----------R 243

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 278 AVSYHDSGATIQFDSSWG---QGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAARLQPVCGRMEVFSAPGKPAV 354
Cdd:COG0773   244 AENIRIDGGGSTFDVLRRgeeLGEVELNLPGRHNVLNALAAIAVALELGVDPEAIAEALASFKGVKRRFELKGEVGGVTV 323

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 355 VVDYAHTPDALEKALQAAR-LHCSGKLWCVFgcggdrdkgkRP----LMGAIAEEF------ADIVVVTDDNPRTEEPRA 423
Cdd:COG0773   324 IDDYAHHPTEIAATLAAAReKYPDRRLVAVF----------QPhrysRTRDFLDEFaealslADEVILLDIYAAREKPIP 393

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*..
gi 1170956475 424 IIN--DILAGMLDAGH-AKVMEGRAEAVTNAVMQAKENDVVLVAGKG 467
Cdd:COG0773   394 GVSseDLAEAIRKRGKdVVYVPDLDELVEALAEIARPGDVVLTMGAG 440
MurD COG0771
UDP-N-acetylmuramoylalanine-D-glutamate ligase [Cell wall/membrane/envelope biogenesis]; ...
 169-462 9.02e-19

UDP-N-acetylmuramoylalanine-D-glutamate ligase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramoylalanine-D-glutamate ligase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440534 [Multi-domain]  Cd Length: 445  Bit Score: 88.60  E-value: 9.02e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 169 LSSLVEQGATFG--AMEVSSHGLvqHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYS-THHSGQAIVNADDEV 245
Cdd:COG0771   144 LLDLLLEPEPPDvyVLELSSFQL--ETTPSLRPDVAVILNITPDHLDRHGSMEAYAAAKARIFAnQTPDDYAVLNADDPL 221

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 246 GRRWLAKLP-DAVAVSMEDhinPNCHGRWLKTTAVSYHDSGATIqFDSSwgqgeiESRLMGAFNVSNLLLALATLLALGY 324
Cdd:COG0771   222 TRALAEEAKaRVVPFSLKE---PLEGGAGLEDGKLVDRASGEEL-LPVD------DLRLPGRHNLENALAALAAARALGV 291

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 325 PLADLLKTAARLQPVCGRMEVFsapgkpAVV--VDY-----AHTPDALEKALQAarlhCSGKLWCVfgCGGDrDKG--KR 395
Cdd:COG0771   292 PPEAIREALRSFKGLPHRLEFV------AEIngVRFindskATNPDATLAALES----FDGPVVLI--AGGL-DKGadFS 358

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1170956475 396 PLMGAIAEEFADIVVVTDDNPRteepraiINDILAGM-LDAGHAKVMEgraEAVTNAVMQAKENDVVL 462
Cdd:COG0771   359 PLAPAVAERVKAVVLIGEDAEK-------IAAALAGAgVPVVIVETME---EAVAAAAELARPGDVVL 416
murD TIGR01087
UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation ...
 168-464 7.28e-16

UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273436 [Multi-domain]  Cd Length: 433  Bit Score: 79.69  E-value: 7.28e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 168 VLSSLVEQGATFGAMEVSSHGLvqHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYSTHHSGQ-AIVNADDEVG 246
Cdd:TIGR01087 141 ALEVLDQEGAELYVLELSSFQL--ETTESLRPEIALILNISEDHLDWHGSFEDYVAAKLKIFARQTEGDvAVLNADDPRF 218

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 247 RRWLAKLPDAVAVSMEDHInpncHGRWLKttavsYHDSGATIQFDSswgqgeIESRLMGAFNVSNLLLALATLLALGYPL 326
Cdd:TIGR01087 219 ARLAQKSKAQVIWFSVEKD----AERGLC-----IRDGGLYLKPND------LEGSLLGLHNAENILAAIALAKSLGLNL 283

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 327 ADLLKTAARLQPVCGRMEVFSAPGKPAVVVDYAHT-PDALEKALQAAR--LHcsgklWCVfgcGGDrDKG---------- 393
Cdd:TIGR01087 284 EAILEALRSFKGLPHRLEYVGQKNGVHFYNDSKATnVHATLAALSAFDnpVI-----LIV---GGD-DKGadfsplapaa 354

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1170956475 394 -KRP----LMGAIAEEFADIVvvtddnprteePRAIINDILagmldaghAKVMEgraEAVTNAVMQAKENDVVLVA 464
Cdd:TIGR01087 355 aGKVkavlAIGEDAAKIAPLL-----------KEAGLSVYL--------VESLE---EAVQAAREVASPGDVVLLS 408
murF TIGR01143
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the ...
 27-385 1.17e-15

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the strictly bacterial MurF gene of peptidoglycan biosynthesis. This enzyme is almost always UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanyl ligase, but in a few species, MurE adds lysine rather than diaminopimelate. This enzyme acts on the product from MurE activity, and so is also subfamily rather than equivalog. Staphylococcus aureus is an example of species in this MurF protein would differ. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273468 [Multi-domain]  Cd Length: 417  Bit Score: 78.85  E-value: 1.17e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  27 LDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEDgeiremhGVPVIFLSQLNERLSALAgRFYHqps 106
Cdd:TIGR01143   1 TDSRAIKPGDLFIALKGERFDGHDFVEQALAAGAVAVVVDREVGPDN-------GLPQILVDDTLEALQALA-RAKR--- 69

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 107 qqlrlvgvtgtngkttttqllaqwAKLLGETSAVMGTVG--------NGLLDKVVPTENTTGS-AVDVQHVLSSL-VEQG 176
Cdd:TIGR01143  70 ------------------------AKFSGKVIGITGSSGktttkemlAAILSHKYKVFATPGNfNNEIGLPLTLLrAPGD 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 177 ATFGAME--VSSHGLVQHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYS-THHSGQAIVNADDEVGRRWLAKL 253
Cdd:TIGR01143 126 HDYAVLEmgASHPGEIAYLAEIAKPDIAVITNIGPAHLEGFGSLEGIAEAKGEILQgLKENGIAVINADDPAFADLAKRL 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 254 PDAVAVSMEDhinpncHGRWLKTTAVSYHDSGATIqFDSSWGQGEIESRL--MGAFNVSNLLLALATLLALGYPLADLLK 331
Cdd:TIGR01143 206 PNRNILSFGF------EGGDFVAKDISYSALGSTS-FTLVAPGGEFEVSLplLGRHNVMNALAAAALALELGIPLEEIAE 278

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1170956475 332 TAARLQPVCGRMEVFSAPGkpAVVVD--YAHTPDALEKALQAArLHCSGKLWCVFG 385
Cdd:TIGR01143 279 GLAELKLVKGRFEVQTKNG--LTLIDdtYNANPDSMRAALDAL-ARFPGKKILVLG 331
MurF COG0770
UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 28-467 1.82e-13

UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl pentapeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440533 [Multi-domain]  Cd Length: 451  Bit Score: 72.06  E-value: 1.82e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  28 DSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEaqgeaedgeiREM-HGVPVI-------FLSQLnerlsalaG 99
Cdd:COG0770    30 DSRKIKPGDLFVALKGERFDGHDFVAQALAKGAAAALVS----------RPLpADLPLIvvddtlkALQQL--------A 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 100 RFYHqpsQQLRL-------------VgvtgtngktttTQLLAQwakLLGETSAVMGTVGN-----GlldkvVP-----TE 156
Cdd:COG0770    92 AAHR---ARFNIpviaitgsngkttT-----------KEMLAA---VLSTKGKVLATPGNfnneiG-----VPltllrLP 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 157 NTTGSAVdvqhvlsslVEQGAT-FGAMEVSShGLVQHRVAalqfaasVFTNLSRDHLDYHGDMEHYEAAKWLLYS-THHS 234
Cdd:COG0770   150 EDHEFAV---------LEMGMNhPGEIAYLA-RIARPDIA-------VITNIGPAHLEGFGSLEGIARAKGEIFEgLPPG 212

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 235 GQAIVNADDEVGRRWLAKLPDAV-AVSMedhiNPNCHgrwLKTTAVSYHDSGATIQFDSSWGQGEIESRLMGAFNVSNLL 313
Cdd:COG0770   213 GVAVLNADDPLLAALAERAKARVlTFGL----SEDAD---VRAEDIELDEDGTRFTLHTPGGELEVTLPLPGRHNVSNAL 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 314 LALATLLALGYPLADLLKTAARLQPVCGRMEVFSAPGKpAVVVD--YAHTPDALEKALQA-ARLHCSGKLWCVFGC---- 386
Cdd:COG0770   286 AAAAVALALGLDLEEIAAGLAAFQPVKGRLEVIEGAGG-VTLIDdsYNANPDSMKAALDVlAQLPGGGRRIAVLGDmlel 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 387 GGDRDKGKRPLMGAIAEEFADIVVVTDdnprtEEPRAIINdiLAGMLDAGHAkvmEGRAEAVTNAVMQAKENDVVLVagK 466
Cdd:COG0770   365 GEESEELHREVGELAAELGIDRLFTVG-----ELARAIAE--AAGGERAEHF---EDKEELLAALKALLRPGDVVLV--K 432


                  .
gi 1170956475 467 G 467
Cdd:COG0770   433 G 433
Mur_ligase pfam01225
Mur ligase family, catalytic domain; This family contains a number of related ligase enzymes ...
 25-102 3.70e-13

Mur ligase family, catalytic domain; This family contains a number of related ligase enzymes which have EC numbers 6.3.2.*. This family includes: MurC, MurD, MurE, MurF, Mpl and FolC. MurC, MurD, Mure and MurF catalyze consecutive steps in the synthesis of peptidoglycan. Peptidoglycan consists of a sheet of two sugar derivatives, with one of these N-acetylmuramic acid attaching to a small pentapeptide. The pentapeptide is is made of L-alanine, D-glutamic acid, Meso-diaminopimelic acid and D-alanyl alanine. The peptide moiety is synthesized by successively adding these amino acids to UDP-N-acetylmuramic acid. MurC transfers the L-alanine, MurD transfers the D-glutamate, MurE transfers the diaminopimelic acid, and MurF transfers the D-alanyl alanine. This family also includes Folylpolyglutamate synthase that transfers glutamate to folylpolyglutamate.


Pssm-ID: 460121 [Multi-domain]  Cd Length: 84  Bit Score: 64.95  E-value: 3.70e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  25 MTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEDGEIREMH--GVPVIFLSQLNERLSALAGRFY 102
Cdd:pfam01225   5 VGIDGRGMSPGALFLALKGYRVDGSDFIESLIALGAAAVVGHDAANNISPDNPELEaaKVPGIPVIDRREALAELAAAFY 84
cya_phycin_syn TIGR02068
cyanophycin synthetase; Cyanophycin is an insoluble storage polymer for carbon, nitrogen, and ...
 203-463 3.33e-12

cyanophycin synthetase; Cyanophycin is an insoluble storage polymer for carbon, nitrogen, and energy, found in most Cyanobacteria. The polymer has a backbone of L-aspartic acid, with most Asp side chain carboxyl groups attached to L-arginine. The polymer is made by this enzyme, cyanophycin synthetase, and degraded by cyanophycinase. Heterologously expressed cyanophycin synthetase in E. coli produces a closely related, water-soluble polymer with some Arg replaced by Lys. It is unclear whether enzymes that produce soluble cyanophycin-like polymers in vivo in non-Cyanobacterial species should be designated as cyanophycin synthetase itself or as a related enzyme. This model makes the designation as cyanophycin synthetase. Cyanophycin synthesis is analogous to polyhydroxyalkanoic acid (PHA) biosynthesis, except that PHA polymers lack nitrogen and may be made under nitrogen-limiting conditions. [Cellular processes, Biosynthesis of natural products]


Pssm-ID: 273950 [Multi-domain]  Cd Length: 864  Bit Score: 69.04  E-value: 3.33e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 203 VFTNLSRDHL--DYHGDMEHYEAAKWLLY-STHHSGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKTTAV 279
Cdd:TIGR02068 569 VVTNIAGDHLgiGDINTIEDLADVKRVVVeVVLPDGYAVLNADDPMVAAMAEKCKGKIAYFSMDPNNPTVAAHIADGGRA 648

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 280 SYHDSGATiqfdsSWGQGEIESRL------------MGAFNVSNLLLALATLLALGYPLADL---LKT-AARLQPVCGRM 343
Cdd:TIGR02068 649 VYYENGYI-----VIARGGDEVAIariaaipltmggRVAFQIENALAAVAAAWALGVPIELIragIRTfDADAAQAPGRF 723

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 344 EVFSApGKPAVVVDYAHTPDALEKALQAARLHCSGKLWCVFGCGGDR-DKGKRPLMGAIAEEFADIVVVTDDNPRTE--- 419
Cdd:TIGR02068 724 NLFNL-GGAHVLVDYGHNPAAIEAVGAAIRNWPARRRIGVIGGPGDRrDEDLVEQGELLGGAFDQIILKEDDDVRGRprg 802

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....
gi 1170956475 420 EPRAIINDILAGMLDAGHAKVMEGRAEAVTNAVMQAKENDVVLV 463
Cdd:TIGR02068 803 EAAALLRQGLRQSARKAAIEDILDETEAIAAALDDLRAGDLVVI 846
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 24-374 6.12e-12

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 68.19  E-value: 6.12e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475  24 EMTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAIIAEAQGEAEDgeiremhgVPVIFLSQLNERLSALAGRFYH 103
Cdd:PRK11929  528 AVSTDSRSVGRGELFVALRGENFDGHDYLPQAFAAGACAAVVERQVADVD--------LPQIVVDDTRAALGRLATAWRA 599

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 104 QpsQQLRLVGVTGTNGKTTTTQLLAQ-WAKLLGEtSAVMGTVGN-----GLLDKVVPTENTTGSAVdvqhvlsslVEQGA 177
Cdd:PRK11929  600 R--FSLPVVAITGSNGKTTTKEMIAAiLAAWQGE-DRVLATEGNfnneiGVPLTLLRLRAQHRAAV---------FELGM 667

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 178 TF-GAMEVSShGLVQHRVAALqfaasvfTNLSRDHLDYHGDMEHYEAAKWLLYST-HHSGQAIVNADDEVGRRWLAKLPD 255
Cdd:PRK11929  668 NHpGEIAYLA-AIAAPTVALV-------TNAQREHQEFMHSVEAVARAKGEIIAAlPEDGVAVVNGDDPYTAIWAKLAGA 739

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 256 AVAVSMEDHINPNCHGRWLKTTAVSYHDSGATIQFDSSWGQGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAAR 335
Cdd:PRK11929  740 RRVLRFGLQPGADVYAEKIAKDISVGEAGGTRCQVVTPAGSAEVYLPLIGEHNLRNALAAIACALAAGASLKQIRAGLER 819

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|.
gi 1170956475 336 LQPVCGRMEVFSAPGKPAVVVD-YAHTPDALEKALQA-ARL 374
Cdd:PRK11929  820 FQPVAGRMQRRRLSCGTRIIDDtYNANPDSMRAAIDVlAEL 860
murC TIGR01082
UDP-N-acetylmuramate--L-alanine ligase; This model describes the MurC protein in bacterial ...
 197-467 1.28e-11

UDP-N-acetylmuramate--L-alanine ligase; This model describes the MurC protein in bacterial peptidoglycan (murein) biosynthesis. In a few species (Mycobacterium leprae, the Chlamydia), the amino acid may be L-serine or glycine instead of L-alanine. A related protein, UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (murein tripeptide ligase) is described by model TIGR01081. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273433 [Multi-domain]  Cd Length: 448  Bit Score: 66.56  E-value: 1.28e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 197 LQFAASVFTNLSRDHLD-YHGDMEHYEAAkWLLYSTHHS--GQAIVNADDEVgrrwLAKLpdaVAVSMEDHINPNCHGRW 273
Cdd:TIGR01082 165 LQPNVAIVTNIEPDHLDtYGSSFERLKAA-FEKFIHNLPfyGLAVICADDPV----LREL---VPKATEQVITYGGSGED 236

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 274 LKTTAVSYHDSGATIQFD---SSWGQGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAARLQPVCGRMEVFSAPG 350
Cdd:TIGR01082 237 ADYRAENIQQSGAEGKFSvrgKGKLYLEFTLNLPGRHNVLNALAAIAVALELGIDFEAILRALANFQGVKRRFEILGEFG 316

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 351 KPAVVVDYAHTPDALEKALQAARLHCSGK-LWCVFgcGGDRDKGKRPLMGAIAEEF--ADIVVVTDDNPRTEEPraiIND 427
Cdd:TIGR01082 317 GVLLIDDYAHHPTEIKATLKAARQGYPDKrIVVVF--QPHRYSRTRDLFDDFAKVLsdADELILLDIYAAGEEP---ING 391

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*...
gi 1170956475 428 ILAGMLdAGHAKVMEGRAEAVTNAVMQ--------AKENDVVLVAGKG 467
Cdd:TIGR01082 392 IDGKSL-ARKITQLGKIEPYFVPDLAElveflaavLQSGDLILTMGAG 438
murD PRK14106
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional
 120-462 3.27e-03

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional


Pssm-ID: 184511 [Multi-domain]  Cd Length: 450  Bit Score: 39.95  E-value: 3.27e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 120 KTTTTQLLAQWAKLLGETSAVMGTVGNGLLDKVvptENTTGSAVDVqhvlsslveqgatfgaMEVSSHGLvqHRVAALQF 199
Cdd:PRK14106  119 KTTTTTLLGEIFKNAGRKTLVAGNIGYPLIDAV---EEYGEDDIIV----------------AEVSSFQL--ETIKEFKP 177

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 200 AASVFTNLSRDHLDYHGDMEHYEAAKWLLYSTHH-SGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNchGRWLKtta 278
Cdd:PRK14106  178 KVGCILNITPDHLDRHKTMENYIKAKARIFENQRpSDYTVLNYDDPRTRSLAKKAKARVIFFSRKSLLEE--GVFVK--- 252

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 279 vsyhdsGATIQFDSSWGQGEI----ESRLMGAFNVSNLLLALATLLALGYPLADLLKTAARLQPVCGRMEVFSAPGKPAV 354
Cdd:PRK14106  253 ------NGKIVISLGGKEEEVididEIFIPGEHNLENALAATAAAYLLGISPDVIANTLKTFKGVEHRIEFVAEINGVKF 326

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1170956475 355 VVDYAHT-PDALEKALQAArlhcsgKLWCVFGCGGdRDKGK--RPLMGAIAEEFADIVVV--TDDNprteepraiindIL 429
Cdd:PRK14106  327 INDSKGTnPDAAIKALEAY------ETPIVLIAGG-YDKGSdfDEFAKAFKEKVKKLILLgeTAQE------------IA 387

                         330       340       350
                  ....*....|....*....|....*....|....*
gi 1170956475 430 AGMLDAGHAKVM--EGRAEAVTNAVMQAKENDVVL 462
Cdd:PRK14106  388 EAARKYGFDNILfaETLEEAVKKAYEIAKPGDVVL 422
murF PRK10773
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; Reviewed
 22-64 6.81e-03

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; Reviewed


Pssm-ID: 182718 [Multi-domain]  Cd Length: 453  Bit Score: 38.86  E-value: 6.81e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 1170956475  22 LREMTLDSRVAASGDLFIAVQGHQADGRRYIPQAIAQGVAAII 64
Cdd:PRK10773   24 IDAVTTDTRKVTPGCLFVALKGERFDAHDFADDAKAAGAGALL 66
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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