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Conserved domains on  [gi|1586923480|gb|TCC91280|]
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NADH-quinone oxidoreductase subunit NuoE [Citrobacter braakii]

Protein Classification

NADH-quinone oxidoreductase subunit NuoE( domain architecture ID 10792932)

NADH-quinone oxidoreductase subunit NuoE is a thioredoxin-like ferredoxin subunit that is involved in the reversible oxidation of molecular hydrogen using NADH or NADPH as electron donor or acceptor

EC:  7.1.1.-
Gene Symbol:  nuoE
Gene Ontology:  GO:0051537|GO:0016491|GO:0030964|GO:0048038
PubMed:  15916556

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK07539 PRK07539
NADH-quinone oxidoreductase subunit NuoE;
14-166 3.52e-97

NADH-quinone oxidoreductase subunit NuoE;


:

Pssm-ID: 181024  Cd Length: 154  Bit Score: 277.04  E-value: 3.52e-97
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  14 LSAAEREAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCD 93
Cdd:PRK07539    2 LSAEELAAIEREIAKYPRPRSAVIPALKIVQEQRGWVPDEAIEAVADYLGMPAIDVEEVATFYSMIFRQPVGRHVIQVCT 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1586923480  94 SVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERYK 166
Cdd:PRK07539   82 STPCWLRGGEAILAALKKKLGIKPGETTADGRFTLLEVECLGACDNAPVVMINDDTYEDLTPEKIDELLDELK 154
 
Name Accession Description Interval E-value
PRK07539 PRK07539
NADH-quinone oxidoreductase subunit NuoE;
14-166 3.52e-97

NADH-quinone oxidoreductase subunit NuoE;


Pssm-ID: 181024  Cd Length: 154  Bit Score: 277.04  E-value: 3.52e-97
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  14 LSAAEREAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCD 93
Cdd:PRK07539    2 LSAEELAAIEREIAKYPRPRSAVIPALKIVQEQRGWVPDEAIEAVADYLGMPAIDVEEVATFYSMIFRQPVGRHVIQVCT 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1586923480  94 SVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERYK 166
Cdd:PRK07539   82 STPCWLRGGEAILAALKKKLGIKPGETTADGRFTLLEVECLGACDNAPVVMINDDTYEDLTPEKIDELLDELK 154
NuoE COG1905
NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; ...
 13-166 2.21e-77

NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) is part of the Pathway/BioSystem: NADH dehydrogenase


Pssm-ID: 441509  Cd Length: 159  Bit Score: 227.33  E-value: 2.21e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  13 ELSAAEREAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYC 92
Cdd:COG1905     5 ELSEELLAEIDEIIAKYPRKRSALIPILHLVQEEFGYLPEEAIEEIAEALGLPPAEVYGVATFYSMFRLKPVGKHVIRVC 84

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1586923480  93 DSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERYK 166
Cdd:COG1905    85 TGTACHLRGAEELLEALEEKLGIKPGETTADGKFTLEEVECLGACGLAPVMMVNDDVYGRLTPEKVDEILDELR 158
2Fe-2S_thioredx pfam01257
Thioredoxin-like [2Fe-2S] ferredoxin;
22-166 2.96e-75

Thioredoxin-like [2Fe-2S] ferredoxin;


Pssm-ID: 460139  Cd Length: 145  Bit Score: 221.19  E-value: 2.96e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  22 IEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHING 101
Cdd:pfam01257   1 IKEIIAKYPRKRSALIPILHLAQEEYGYLPPEAIEYVAELLGIPPARVYEVATFYTMFSLKPVGKYHIQVCTGTPCHLRG 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1586923480 102 YQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERYK 166
Cdd:pfam01257  81 SDEILEALEKKLGIKPGETTPDGKFTLEEVECLGACDNAPVMQINDDYYGDLTPEKVDEILEELK 145
nuoE_fam TIGR01958
NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that ...
 21-166 6.40e-71

NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that resemble NADH-quinone oxidoreductases. The electron acceptor is a quinone, ubiquinone, in mitochondria and most bacteria, including Escherichia coli, where the recommended gene symbol is nuoB. This model does not identify proteins from chloroplast and cyanobacteria. [Energy metabolism, Electron transport]


Pssm-ID: 131013  Cd Length: 148  Bit Score: 210.69  E-value: 6.40e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  21 AIEHEKHHYEDP--RAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCH 98
Cdd:TIGR01958   1 LIEEIIAKYPDDqkRSAIMPALMIAQEQKGWVTPEAIAAVAEMLGIPPVWVYEVATFYSMFDTEPVGRYHLQVCTNVPCA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1586923480  99 INGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERYK 166
Cdd:TIGR01958  81 LRGSEALLKYLENKLGIKPGETTPDGRFTLVEVECLGACGNAPVMMINDDYYEFLTPEKLDELLERYE 148
TRX_Fd_NuoE cd03064
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 86-165 4.51e-39

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily; Nuo, also called respiratory chain Complex 1, is the entry point for electrons into the respiratory chains of bacteria and the mitochondria of eukaryotes. It is a multisubunit complex with at least 14 core subunits. It catalyzes the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane, providing the proton motive force required for energy-consuming processes. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster present in NuoE core subunit, also called the 24 kD subunit of Complex 1. This subfamily also include formate dehydrogenases, NiFe hydrogenases and NAD-reducing hydrogenases, that contain a NuoE domain. A subset of these proteins contain both NuoE and NuoF in a single chain. NuoF, also called the 51 kD subunit of Complex 1, contains one [4Fe-4S] cluster and also binds the NADH substrate and FMN.


Pssm-ID: 239362 [Multi-domain]  Cd Length: 80  Bit Score: 127.63  E-value: 4.51e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  86 RHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERY 165
Cdd:cd03064     1 KHVIRVCTGTACHLRGAEALLEALEKKLGIKPGETTPDGRFTLEEVECLGACDLAPVMMINDDVYGRLTPEKVDAILEAL 80
 
Name Accession Description Interval E-value
PRK07539 PRK07539
NADH-quinone oxidoreductase subunit NuoE;
14-166 3.52e-97

NADH-quinone oxidoreductase subunit NuoE;


Pssm-ID: 181024  Cd Length: 154  Bit Score: 277.04  E-value: 3.52e-97
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  14 LSAAEREAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCD 93
Cdd:PRK07539    2 LSAEELAAIEREIAKYPRPRSAVIPALKIVQEQRGWVPDEAIEAVADYLGMPAIDVEEVATFYSMIFRQPVGRHVIQVCT 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1586923480  94 SVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERYK 166
Cdd:PRK07539   82 STPCWLRGGEAILAALKKKLGIKPGETTADGRFTLLEVECLGACDNAPVVMINDDTYEDLTPEKIDELLDELK 154
NuoE COG1905
NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; ...
 13-166 2.21e-77

NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) is part of the Pathway/BioSystem: NADH dehydrogenase


Pssm-ID: 441509  Cd Length: 159  Bit Score: 227.33  E-value: 2.21e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  13 ELSAAEREAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYC 92
Cdd:COG1905     5 ELSEELLAEIDEIIAKYPRKRSALIPILHLVQEEFGYLPEEAIEEIAEALGLPPAEVYGVATFYSMFRLKPVGKHVIRVC 84

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1586923480  93 DSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERYK 166
Cdd:COG1905    85 TGTACHLRGAEELLEALEEKLGIKPGETTADGKFTLEEVECLGACGLAPVMMVNDDVYGRLTPEKVDEILDELR 158
2Fe-2S_thioredx pfam01257
Thioredoxin-like [2Fe-2S] ferredoxin;
22-166 2.96e-75

Thioredoxin-like [2Fe-2S] ferredoxin;


Pssm-ID: 460139  Cd Length: 145  Bit Score: 221.19  E-value: 2.96e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  22 IEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHING 101
Cdd:pfam01257   1 IKEIIAKYPRKRSALIPILHLAQEEYGYLPPEAIEYVAELLGIPPARVYEVATFYTMFSLKPVGKYHIQVCTGTPCHLRG 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1586923480 102 YQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERYK 166
Cdd:pfam01257  81 SDEILEALEKKLGIKPGETTPDGKFTLEEVECLGACDNAPVMQINDDYYGDLTPEKVDEILEELK 145
nuoE_fam TIGR01958
NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that ...
 21-166 6.40e-71

NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that resemble NADH-quinone oxidoreductases. The electron acceptor is a quinone, ubiquinone, in mitochondria and most bacteria, including Escherichia coli, where the recommended gene symbol is nuoB. This model does not identify proteins from chloroplast and cyanobacteria. [Energy metabolism, Electron transport]


Pssm-ID: 131013  Cd Length: 148  Bit Score: 210.69  E-value: 6.40e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  21 AIEHEKHHYEDP--RAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCH 98
Cdd:TIGR01958   1 LIEEIIAKYPDDqkRSAIMPALMIAQEQKGWVTPEAIAAVAEMLGIPPVWVYEVATFYSMFDTEPVGRYHLQVCTNVPCA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1586923480  99 INGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERYK 166
Cdd:TIGR01958  81 LRGSEALLKYLENKLGIKPGETTPDGRFTLVEVECLGACGNAPVMMINDDYYEFLTPEKLDELLERYE 148
TRX_Fd_NuoE cd03064
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 86-165 4.51e-39

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily; Nuo, also called respiratory chain Complex 1, is the entry point for electrons into the respiratory chains of bacteria and the mitochondria of eukaryotes. It is a multisubunit complex with at least 14 core subunits. It catalyzes the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane, providing the proton motive force required for energy-consuming processes. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster present in NuoE core subunit, also called the 24 kD subunit of Complex 1. This subfamily also include formate dehydrogenases, NiFe hydrogenases and NAD-reducing hydrogenases, that contain a NuoE domain. A subset of these proteins contain both NuoE and NuoF in a single chain. NuoF, also called the 51 kD subunit of Complex 1, contains one [4Fe-4S] cluster and also binds the NADH substrate and FMN.


Pssm-ID: 239362 [Multi-domain]  Cd Length: 80  Bit Score: 127.63  E-value: 4.51e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  86 RHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERY 165
Cdd:cd03064     1 KHVIRVCTGTACHLRGAEALLEALEKKLGIKPGETTPDGRFTLEEVECLGACDLAPVMMINDDVYGRLTPEKVDAILEAL 80
PRK05988 PRK05988
formate dehydrogenase subunit gamma; Validated
 7-164 6.81e-28

formate dehydrogenase subunit gamma; Validated


Pssm-ID: 180339  Cd Length: 156  Bit Score: 101.57  E-value: 6.81e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480   7 PQTEAFELSAAEReAIEHEKHHyedpRAASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGR 86
Cdd:PRK05988    1 MPDEPWDAARIAA-IIAEHKHL----EGALLPILHAIQDEFGYVPEDAVPVIAEALNLSRAEVHGVITFYHDFRTHPPGR 75

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1586923480  87 HVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLER 164
Cdd:PRK05988   76 HVLKLCRAEACQAMGGDALAAHAKARLGIDFHQTTADGAVTLEPVYCLGLCACSPAAMLDGEVHGRLDPQRLDALLAE 153
PRK07571 PRK07571
bidirectional hydrogenase complex protein HoxE; Reviewed
 35-166 7.42e-27

bidirectional hydrogenase complex protein HoxE; Reviewed


Pssm-ID: 236053  Cd Length: 169  Bit Score: 99.06  E-value: 7.42e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  35 ASIEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGYQGIQAALEKKLN 114
Cdd:PRK07571   37 ALIEVLHKAQELFGYLERDLLLYVARQLKLPLSRVYGVATFYHLFSLKPSGEHTCVVCTGTACYVKGSAAILEDLENELG 116

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1586923480 115 IKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLERYK 166
Cdd:PRK07571  117 IKAGETTADGKLSLLTARCLGACGIAPAVVFDGKVAGKQTPESVLEKVQGWL 168
PRK12373 PRK12373
NADH-quinone oxidoreductase subunit E;
8-165 3.05e-24

NADH-quinone oxidoreductase subunit E;


Pssm-ID: 237082 [Multi-domain]  Cd Length: 400  Bit Score: 96.79  E-value: 3.05e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480   8 QTEAFELSAAEREAIEHEKHHYEDPRAAS--IEALKIVQKQRGWVPDGAIYAIADVLGIPASDVEGVATFYSQIFRQPVG 85
Cdd:PRK12373    9 QPDSFAFTPENAAWAEKQITKYPEGRQASavIPLLMRAQEQEGWVTRAAIEKVADMLDMAYIRVLEVATFYTQFQLQPVG 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  86 -RHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLER 164
Cdd:PRK12373   89 tRAHIQVCGTTPCMLRGSEALMAVCKSKIHAHPHELNADGTLSWEEVECLGACVNAPMVQIGKDYYEDLTPERLEEIIDA 168


                  .
gi 1586923480 165 Y 165
Cdd:PRK12373  169 F 169
TRX_Fd_family cd02980
Thioredoxin (TRX)-like [2Fe-2S] Ferredoxin (Fd) family; composed of [2Fe-2S] Fds with a TRX ...
 87-165 9.27e-23

Thioredoxin (TRX)-like [2Fe-2S] Ferredoxin (Fd) family; composed of [2Fe-2S] Fds with a TRX fold (TRX-like Fds) and proteins containing domains similar to TRX-like Fd including formate dehydrogenases, NAD-reducing hydrogenases and the subunit E of NADH:ubiquinone oxidoreductase (NuoE). TRX-like Fds are soluble low-potential electron carriers containing a single [2Fe-2S] cluster. The exact role of TRX-like Fd is still unclear. It has been suggested that it may be involved in nitrogen fixation. Its homologous domains in large redox enzymes (such as Nuo and hydrogenases) function as electron carriers.


Pssm-ID: 239278 [Multi-domain]  Cd Length: 77  Bit Score: 85.76  E-value: 9.27e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  87 HVIRYCDSVVCHINGYQGIQAALEKKLNIKPGqttfDGRFTLLPTCCLGNCDKGPNMMI--DEDTHAHLTPEGIPELLER 164
Cdd:cd02980     1 HHILVCTGTACGLRGAEELLEALEKELGIRGG----DGRVTVERVGCLGACGLAPVVVVypDGVWYGRVTPEDVEEIVEE 76


                  .
gi 1586923480 165 Y 165
Cdd:cd02980    77 L 77
TRX_Fd_NuoE_hoxF cd03083
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 89-163 1.14e-19

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily, hoxF; composed of proteins similar to the NAD-reducing hydrogenase (hoxS) alpha subunit of Alcaligenes eutrophus H16. HoxS is a cytoplasmic hydrogenase catalyzing the oxidation of molecular hydrogen accompanied by the reduction of NAD. It is composed of four structural subunits encoded by the genes hoxF, hoxU, hoxY and hoxH. The hoxF protein (or alpha subunit) is a fusion protein containing an N-terminal NuoE-like domain and a C-terminal NuoF domain. NuoE and NuoF are components of Nuo, a multisubunit complex catalyzing the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster in NuoE and the [4Fe-4S] cluster in NuoF. In addition, NuoF is also the NADH- and FMN-binding subunit. HoxF may be involved in the electron transport chain during the NAD-dependent oxidation of hydrogen through its NuoF domain. The NuoE-like domain of hoxF contains only one conserved cysteine in its putative active site, compared to four cysteines in NuoE, and may have lost the ability to bind [2Fe-2S] clusters.


Pssm-ID: 239381  Cd Length: 80  Bit Score: 78.22  E-value: 1.14e-19
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1586923480  89 IRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLE 163
Cdd:cd03083     4 IYLSDSITDRMNGYKAVLDALCRELGIRFGEVDEDGMVGLFFTSCTGLCDQGPALLINNRVFTRLTPGRIDQIAE 78
TRX_Fd_NuoE_FDH_gamma cd03081
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 86-164 3.70e-18

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily, NAD-dependent formate dehydrogenase (FDH) gamma subunit; composed of proteins similar to the gamma subunit of NAD-linked FDH of Ralstonia eutropha, a soluble enzyme that catalyzes the irreversible oxidation of formate to carbon dioxide accompanied by the reduction of NAD+ to NADH. FDH is a heteromeric enzyme composed of four nonidentical subunits (alpha, beta, gamma and delta). The FDH gamma subunit is closely related to NuoE, which is part of a multisubunit complex (Nuo) catalyzing the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster present in NuoE. Similarly, the FDH gamma subunit is hypothesized to be involved in an electron transport chain involving other FDH subunits, upon the oxidation of formate.


Pssm-ID: 239379  Cd Length: 80  Bit Score: 74.34  E-value: 3.70e-18
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1586923480  86 RHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEGIPELLER 164
Cdd:cd03081     1 RHVLKLCRAEACQAMGAEALAAHIKARLGIDFHETTADGSVTLEPVYCLGLCACSPAAMIDGEVHGRVDPEKFDALLAE 79
COG3411 COG3411
2Fe-2S ferredoxin [Energy production and conversion]; 2Fe-2S ferredoxin is part of the Pathway ...
 86-165 5.60e-04

2Fe-2S ferredoxin [Energy production and conversion]; 2Fe-2S ferredoxin is part of the Pathway/BioSystem: 2Fe2S


Pssm-ID: 442637  Cd Length: 96  Bit Score: 37.18  E-value: 5.60e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1586923480  86 RHVIRYCDSVvCHINGYQGIQAALE---KKLNIkpgqttfDGRFTLLPTCCLGNCDKGPNMMI--DEDTHAHLTPEGIPE 160
Cdd:COG3411     5 RHHVLVCTGS-CGAAGAEEVLDALKeelKERGL-------DGDVRVTKTGCLGRCEQGPVVVVypEGVWYGNVTPEDVPE 76


                  ....*
gi 1586923480 161 LLERY 165
Cdd:COG3411    77 IVEEH 81
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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