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Conserved domains on  [gi|1633077807|gb|TKF52794|]
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curli production assembly protein CsgF [Vibrio sp. F13]

Protein Classification

curli production assembly/transport component CsgF( domain architecture ID 10846)

curli production assembly/transport component CsgF may be involved in the biogenesis of curli organelles

Gene Ontology:  GO:0042802|GO:0098775|GO:0098777
TCDB:  1.B.48.1.1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CsgF super family cl08082
Type VIII secretion system (T8SS), CsgF protein; The extracellular nucleation-precipitation ...
 3-132 5.14e-26

Type VIII secretion system (T8SS), CsgF protein; The extracellular nucleation-precipitation (ENP) pathway or Type VIII secretion system (T8SS) in Gram-negative (diderm) bacteria is responsible for the secretion and assembly of prepilins for fimbiae biogenesis, the prototypical curli. Besides the T2SS that can be involved in the assembly of prototypical Type 4 pilus, the T4SS that can be involved in the biogenesis of the prototypical pilus T, the T3SS involved in the assembly of the injectisome and the T7SS involved in the formation of the prototypical Type 1 pilus, the T8SS differs in that fibre-growth occurs extracellularly. The curli, also called thin aggregative fimbriae (Tafi), are the only fimbriae dependent on the T8SS. Tafi were first identified in Salmonella spp and the controlling operon termed agf; however subsequent isolation of the homologous operon in E coli led to its being called csg. In the absence of extracellular polysaccharides Tafi appear curled, although when expressed with such polysaccharides their morphology appears as a tangled amorphous matrix. CsgF is one of three putative curli assembly factors appearing to act as a nucleator protein. Unlike eukaryotic amyloid formation, curli biogenesis is a productive pathway requiring a specific assembly machinery.


The actual alignment was detected with superfamily member PRK10050:

Pssm-ID: 447570  Cd Length: 138  Bit Score: 95.04  E-value: 5.14e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1633077807   3 ICFRKAVLIVLLASPSVYSSELVYTPVNPSFGGNALNSSHLFNHANAINDFEDPSARDIFD-EQESALDRLASSLESRLI 81
Cdd:PRK10050    1 MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNSAQAQNSYKDPSYNDDFGiETPSALDNFTQAIQSQIL 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1633077807  82 SQLLADVGNGNTGQLETDDFFLNIVDDSGTLLVQIVDKATGESTEISVSGL 132
Cdd:PRK10050   81 GGLLSNINTGKPGRMVTNDYIVDIANRDGQLQLNVTDRKTGQTSTIQVSGL 131
 
Name Accession Description Interval E-value
PRK10050 PRK10050
curli production assembly/transport protein CsgF;
3-132 5.14e-26

curli production assembly/transport protein CsgF;


Pssm-ID: 182210  Cd Length: 138  Bit Score: 95.04  E-value: 5.14e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1633077807   3 ICFRKAVLIVLLASPSVYSSELVYTPVNPSFGGNALNSSHLFNHANAINDFEDPSARDIFD-EQESALDRLASSLESRLI 81
Cdd:PRK10050    1 MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNSAQAQNSYKDPSYNDDFGiETPSALDNFTQAIQSQIL 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1633077807  82 SQLLADVGNGNTGQLETDDFFLNIVDDSGTLLVQIVDKATGESTEISVSGL 132
Cdd:PRK10050   81 GGLLSNINTGKPGRMVTNDYIVDIANRDGQLQLNVTDRKTGQTSTIQVSGL 131
CsgF pfam10614
Type VIII secretion system (T8SS), CsgF protein; The extracellular nucleation-precipitation ...
 8-130 1.87e-22

Type VIII secretion system (T8SS), CsgF protein; The extracellular nucleation-precipitation (ENP) pathway or Type VIII secretion system (T8SS) in Gram-negative (diderm) bacteria is responsible for the secretion and assembly of prepilins for fimbiae biogenesis, the prototypical curli. Besides the T2SS that can be involved in the assembly of prototypical Type 4 pilus, the T4SS that can be involved in the biogenesis of the prototypical pilus T, the T3SS involved in the assembly of the injectisome and the T7SS involved in the formation of the prototypical Type 1 pilus, the T8SS differs in that fibre-growth occurs extracellularly. The curli, also called thin aggregative fimbriae (Tafi), are the only fimbriae dependent on the T8SS. Tafi were first identified in Salmonella spp and the controlling operon termed agf; however subsequent isolation of the homologous operon in E coli led to its being called csg. In the absence of extracellular polysaccharides Tafi appear curled, although when expressed with such polysaccharides their morphology appears as a tangled amorphous matrix. CsgF is one of three putative curli assembly factors appearing to act as a nucleator protein. Unlike eukaryotic amyloid formation, curli biogenesis is a productive pathway requiring a specific assembly machinery.


Pssm-ID: 431395  Cd Length: 132  Bit Score: 85.93  E-value: 1.87e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1633077807   8 AVLIVLLASPSVYS--SELVYTPVNPSFGGNALNSSHLFNHANAINDFEDPSArDIFDEQESALDRLASSLESRLISQL- 84
Cdd:pfam10614   2 AVALLLLGLAASPAkaQELVYQPTNPAFGGNPLNGSWLLSSAQAQNSFKDPAY-DSGRQSLSPLDDFADQLQRQLLSRLs 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1633077807  85 ---LADVGNGNT---GQLETDDFFLNIVDDSGTLLVQIVDKATGESTEISVS 130
Cdd:pfam10614  81 dkiVQQQGGENTlpeGTYTFGSYQISITNSGSGVSITITDGSTGESTTVTVP 132
 
Name Accession Description Interval E-value
PRK10050 PRK10050
curli production assembly/transport protein CsgF;
3-132 5.14e-26

curli production assembly/transport protein CsgF;


Pssm-ID: 182210  Cd Length: 138  Bit Score: 95.04  E-value: 5.14e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1633077807   3 ICFRKAVLIVLLASPSVYSSELVYTPVNPSFGGNALNSSHLFNHANAINDFEDPSARDIFD-EQESALDRLASSLESRLI 81
Cdd:PRK10050    1 MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNSAQAQNSYKDPSYNDDFGiETPSALDNFTQAIQSQIL 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1633077807  82 SQLLADVGNGNTGQLETDDFFLNIVDDSGTLLVQIVDKATGESTEISVSGL 132
Cdd:PRK10050   81 GGLLSNINTGKPGRMVTNDYIVDIANRDGQLQLNVTDRKTGQTSTIQVSGL 131
CsgF pfam10614
Type VIII secretion system (T8SS), CsgF protein; The extracellular nucleation-precipitation ...
 8-130 1.87e-22

Type VIII secretion system (T8SS), CsgF protein; The extracellular nucleation-precipitation (ENP) pathway or Type VIII secretion system (T8SS) in Gram-negative (diderm) bacteria is responsible for the secretion and assembly of prepilins for fimbiae biogenesis, the prototypical curli. Besides the T2SS that can be involved in the assembly of prototypical Type 4 pilus, the T4SS that can be involved in the biogenesis of the prototypical pilus T, the T3SS involved in the assembly of the injectisome and the T7SS involved in the formation of the prototypical Type 1 pilus, the T8SS differs in that fibre-growth occurs extracellularly. The curli, also called thin aggregative fimbriae (Tafi), are the only fimbriae dependent on the T8SS. Tafi were first identified in Salmonella spp and the controlling operon termed agf; however subsequent isolation of the homologous operon in E coli led to its being called csg. In the absence of extracellular polysaccharides Tafi appear curled, although when expressed with such polysaccharides their morphology appears as a tangled amorphous matrix. CsgF is one of three putative curli assembly factors appearing to act as a nucleator protein. Unlike eukaryotic amyloid formation, curli biogenesis is a productive pathway requiring a specific assembly machinery.


Pssm-ID: 431395  Cd Length: 132  Bit Score: 85.93  E-value: 1.87e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1633077807   8 AVLIVLLASPSVYS--SELVYTPVNPSFGGNALNSSHLFNHANAINDFEDPSArDIFDEQESALDRLASSLESRLISQL- 84
Cdd:pfam10614   2 AVALLLLGLAASPAkaQELVYQPTNPAFGGNPLNGSWLLSSAQAQNSFKDPAY-DSGRQSLSPLDDFADQLQRQLLSRLs 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1633077807  85 ---LADVGNGNT---GQLETDDFFLNIVDDSGTLLVQIVDKATGESTEISVS 130
Cdd:pfam10614  81 dkiVQQQGGENTlpeGTYTFGSYQISITNSGSGVSITITDGSTGESTTVTVP 132
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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