forkhead associated (FHA) domain found in protein phosphatase 1 regulatory inhibitor subunit 8 (PPP1R8) and similar proteins; PPP1R8, also called nuclear inhibitor of protein phosphatase 1 (NIPP-1), is an inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. It may also be involved in pre-mRNA splicing and binds DNA and might act as a transcriptional repressor. PPP1R8 seems to be required for cell proliferation. PPP1R8 contains an FHA domain that mediates interactions with threonine-phosphorylated maternal embryonic leucine zipper kinase (MELK). The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275   3 LKDLVSLQKLIIDEKKFYLFGRNPDICDFTIDHQSCSRVHAALVYHKHLKRLFLIDLNSTHGTFLGHIRLEPHKPQQVPI 82
Cdd:cd22674    12 VKDGKLIEKLMIDEKKYYLFGRNSDVCDFVLDHPSCSRVHAALVYHKHLNRVFLIDLGSTHGTFVGGIRLEPHKPQQLPI 91

                          90
                  ....*....|....*..
gi 1709635275  83 DSTMSFGASTRVYTIRE 99
Cdd:cd22674    92 DSTLRFGASTRRYILRE 108

forkhead associated (FHA) domain found in protein phosphatase 1 regulatory inhibitor subunit 8 (PPP1R8) and similar proteins; PPP1R8, also called nuclear inhibitor of protein phosphatase 1 (NIPP-1), is an inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. It may also be involved in pre-mRNA splicing and binds DNA and might act as a transcriptional repressor. PPP1R8 seems to be required for cell proliferation. PPP1R8 contains an FHA domain that mediates interactions with threonine-phosphorylated maternal embryonic leucine zipper kinase (MELK). The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275   3 LKDLVSLQKLIIDEKKFYLFGRNPDICDFTIDHQSCSRVHAALVYHKHLKRLFLIDLNSTHGTFLGHIRLEPHKPQQVPI 82
Cdd:cd22674    12 VKDGKLIEKLMIDEKKYYLFGRNSDVCDFVLDHPSCSRVHAALVYHKHLNRVFLIDLGSTHGTFVGGIRLEPHKPQQLPI 91

                          90
                  ....*....|....*..
gi 1709635275  83 DSTMSFGASTRVYTIRE 99
Cdd:cd22674    92 DSTLRFGASTRRYILRE 108

Forkhead associated domain; Found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1709635275   20 YLFGRNPDICDFTIDHQSCSRVHAALVYHKHlKRLFLIDLNSTHGTFLGHIRL 72
Cdd:smart00240   1 VTIGRSSEDCDIQLDGPSISRRHAVIVYDGG-GRFYLIDLGSTNGTFVNGKRI 52

antheraxanthin epoxidase/zeaxanthin epoxidase

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275  12 LIIDEKKFYLFGRNPDIcDF-----TIDHQSCSRVHAALVYHKhlKRLFLIDLNSTHGTFLG-----HIRLEPHKPQQVP 81
Cdd:PLN02927  551 LTKDEDQPCIVGSEPDQ-DFpgmriVIPSSQVSKMHARVIYKD--GAFFLMDLRSEHGTYVTdnegrRYRATPNFPARFR 627

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1709635275  82 IDSTMSFG----ASTRVYTIREKPQS-QANVSAGDK 112
Cdd:PLN02927  628 SSDIIEFGsdkkAAFRVKVIRKTPKStRKNESNNDK 663

forkhead associated (FHA) domain found in protein phosphatase 1 regulatory inhibitor subunit 8 (PPP1R8) and similar proteins; PPP1R8, also called nuclear inhibitor of protein phosphatase 1 (NIPP-1), is an inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. It may also be involved in pre-mRNA splicing and binds DNA and might act as a transcriptional repressor. PPP1R8 seems to be required for cell proliferation. PPP1R8 contains an FHA domain that mediates interactions with threonine-phosphorylated maternal embryonic leucine zipper kinase (MELK). The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275   3 LKDLVSLQKLIIDEKKFYLFGRNPDICDFTIDHQSCSRVHAALVYHKHLKRLFLIDLNSTHGTFLGHIRLEPHKPQQVPI 82
Cdd:cd22674    12 VKDGKLIEKLMIDEKKYYLFGRNSDVCDFVLDHPSCSRVHAALVYHKHLNRVFLIDLGSTHGTFVGGIRLEPHKPQQLPI 91

                          90
                  ....*....|....*..
gi 1709635275  83 DSTMSFGASTRVYTIRE 99
Cdd:cd22674    92 DSTLRFGASTRRYILRE 108

forkhead associated (FHA) domain found in kanadaptin and similar proteins; Kanadaptin, also called human lung cancer oncogene 3 protein (HLC-3), kidney anion exchanger adapter protein, or solute carrier family 4 anion exchanger member 1 adapter protein (SLC4A1AP), is a nuclear protein widely expressed in mammalian tissues. It was originally isolated as a kidney Cl-/HCO3- anion exchanger 1 (kAE1)-binding protein. It is a highly mobile nucleocytoplasmic shuttling and multilocalizing protein. Its role in mammalian cells remains unclear. It contains an FHA domain, which is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275   3 LKDLVSLQKLIIDEKKFYLFGRNPDiCDFTIDHQSCSRVHAALVYH----KHLKRLFLIDLNSTHGTFLGHIRLEPHKPQ 78
Cdd:cd22677     7 LKNGVIVETLDLNGKSFYVFGRLPG-CDVVLEHPSISRYHAVLQYRgdadDHDGGFYLYDLGSTHGTFLNKQRIPPKQYY 85

                          90
                  ....*....|....*..
gi 1709635275  79 QVPIDSTMSFGASTRVY 95
Cdd:cd22677    86 RLRVGHVLKFGGSTRLY 102

forkhead associated (FHA) domain found in Arabidopsis thaliana Protein PARALLEL SPINDLE 1 (PS1) and similar proteins; PS1 is an FHA domain-containing protein required for normal spindle orientation at male meiosis II and normal formation of tetrad of microspores. It is not involved in female meiosis. Mutations in PS1 lead to the production of diploid pollen grains. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275  21 LFGRNPDiCDFTIDHQSCSRVHAALVYHKHLKRLFLIDLNSTHGTFLGHIRLEPHKPQQVPIDSTMSFGASTRVYTIREK 100
Cdd:cd22691    32 VVGRHPD-CDIVLDHPSISRFHLEIRIIPSRRKITLTDLSSVHGTWVNGQRIEPGVPVELEEGDTVRLGASTRVYRLHWI 110

                  ...
gi 1709635275 101 PQS 103
Cdd:cd22691   111 PVS 113

forkhead associated (FHA) domain found in Arabidopsis thaliana FHA domain-containing protein DDL and similar proteins; DDL, also called protein DAWDLE, is involved in microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. It may facilitate DCL1 to access or recognize primary miRNAs. DDL binds RNA non-specifically. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275  12 LIIDEKKFYLFGRNPDICDFTIDHQSCSRVHAALVYHKHLK-----------RLFLIDLNSTHGTFLGHIRLEPHKPQQV 80
Cdd:cd22719    31 LHIHRQSCYLFGRERKVADIPTDHPSCSKQHAVIQYRLTEKeggdgmmgkavRPYIMDLGSTNGTFLNGERIEPQRYYEL 110

                          90
                  ....*....|....*..
gi 1709635275  81 PIDSTMSFGASTRVYTI 97
Cdd:cd22719   111 LEKDTIKFGNSSREYVL 127

forkhead associated (FHA) domain superfamily; Forkhead-associated (FHA) domains are small phosphopeptide recognition modules mostly found in eubacteria and eukaryotes. It is about 95-120 residues long that fold into an 11-stranded beta-sandwich. FHA domains can mediate the recognition of phosphorylated and non-phosphorylated substrates, as well as protein oligomerization. They specifically recognize threonine phosphorylation (pThr) accompanying activation of protein serine/threonine kinases. FHA domains show diverse ligand specificity. They may recognize the pTXXD motif, the pTXXI/L motif, and TQ clusters (singly and multiply phosphorylated). In eukaryotes, FHA superfamily members include forkhead-type transcription factors, as well as other signaling proteins, such as many regulatory proteins, kinases, phosphatases, motor proteins called kinesins, and metabolic enzymes. Many of them localize to the nucleus, where they participate in establishing or maintaining cell cycle checkpoints, DNA repair, or transcriptional regulation. FHA domains play important roles in human diseases, particularly in relation to DNA damage responses and cancers. In bacteria, FHA domain-containing proteins may participate in injection of viral proteins into host cells, transmembrane transporters, and cell division. FHA domain-containing proteins rarely include more than one copy of the domain. The only exception in eukaryotes is the checkpoint kinase Rad53 from Saccharomyces cerevisiae, which harbors two FHA domains (FHA1 and FHA2) flanking a central kinase domain. The two FHA domains recognize different phosphorylated targets and function independently from one another. In contrast, Mycobacterium tuberculosis ABC transporter Rv1747 contains two FHA domains but only one of them is essential for protein function.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1709635275  16 EKKFYLFGRNPDiCDFTIDHQSCSRVHAALVYHKhlKRLFLIDLNSTHGTFLGHIRLEPhkPQQVPIDSTMSFGAST 92
Cdd:cd00060    17 TKGVVTIGRSPD-CDIVLDDPSVSRRHARIEVDG--GGVYLEDLGSTNGTFVNGKRITP--PVPLQDGDVIRLGDTT 88

Forkhead associated (FHA) domain, binds pSer, pThr, pTyr [Signal transduction mechanisms];

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1709635275  20 YLFGRNPDiCDFTIDHQSCSRVHAALVYHKHlkRLFLIDLNSTHGTFLGHIRLEphKPQQVPIDSTMSFGASTRVYTIR 98
Cdd:COG1716    23 LTIGRAPD-NDIVLDDPTVSRRHARIRRDGG--GWVLEDLGSTNGTFVNGQRVT--EPAPLRDGDVIRLGKTELRFRLS 96

forkhead associated (FHA) domain found in transcription factor 19 (TCF-19) and similar proteins; TCF-19, also called transcription factor SC1, was identified as a putative trans-activating factor with expression beginning at the late G1-S boundary in dividing cells. It also functions as a novel islet factor necessary for proliferation and survival in the INS-1 beta cell line. It plays an important role in susceptibility to both Type 1 Diabetes Mellitus (T1DM) and Type 2 Diabetes Mellitus (T2DM); it has been suggested that it may positively impact beta cell mass under conditions of beta cell stress and increased insulin demand. TCF-19 contains an N-terminal fork head association domain (FHA), a proline rich region, and a C-terminal plant homeodomain (PHD) finger. The FHA domain may serve as a nuclear signaling domain or as a phosphoprotein binding domain. The proline rich region is a common characteristic of trans-activating factors. The PHD finger may allow TCF-19 to interact with chromatin via methylated histone H3.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275  19 FYLFGRNPDICDFTIDHQSC----SRVHAALvyhkHLKRL------FLIDLNSTHGTFLGHIRLEPHKPQQVPIDSTMSF 88
Cdd:cd22685    29 EYRIGRNPEVCDVFLCSSQHpnliSREHAEI----HAERDgngnwkVLIEDRSTNGTYVNDVRLQDGQRRELSDGDTITF 104

                          90
                  ....*....|....*...
gi 1709635275  89 GASTRVYTIREKPQSQAN 106
Cdd:cd22685   105 GHKNGRRVKQWPYQKSSE 122

forkhead associated (FHA) domain found in nibrin and similar proteins; Nibrin (NBN), also called cell cycle regulatory protein p95, or Nijmegen breakage syndrome protein 1 (NBS1), is a novel DNA double-strand break repair protein that is mutated in Nijmegen breakage syndrome. It is a component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. Nibrin modulates the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. Nibrin also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. Nibrin is a major player in the control of intra-S-phase checkpoint. This subfamily also includes Schizosaccharomyces pombe DNA repair and telomere maintenance protein Nbs1 and Arabidopsis thaliana AtNbs1. SpNbs1 is an FHA domain-containing protein required for DNA damage repair and S-phase DNA damage checkpoint. It is involved in telomere length maintenance and maintenance of chromatin structure. AtNbs1 is a component of MRN complex. It also functions in the very early stages of meiosis. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275  20 YLFGRNpdICDFTIDH-QSCSRVHAALV---------YHKHLKRLFLIDLnSTHGTFLGHIRLEPHKPQQVPIDSTMSFG 89
Cdd:cd22667    22 YTVGRK--DCDIIIVDdSSISRKHATLTvlhpeanlsDPDTRPELTLKDL-SKYGTFVNGEKLKGGSEVTLKDGDVITFG 98

                  ....*....
gi 1709635275  90 ASTRVYTIR 98
Cdd:cd22667    99 VLGSKFRVE 107

forkhead associated (FHA) domain found in the centrosomal protein of 170 kDa protein (Cep170) family; The Cep170 family includes Cep170 and Cep170B. Cep170, also called Cep170A, KARP-1-binding protein, or KARP1-binding protein, is a protein that localizes to centrosomes as well as spindle microtubules and plays a role in microtubule organization and microtubule assembly. It is required for centriole subdistal appendage assembly. Cep170 is phosphorylated during M phase and interacts with Polo-like kinase 1 (Plk1). Cep170B, also called centrosomal protein 170B, plays a role in microtubule organization. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275  21 LFGRnpDICDFTIDHQSCSRVHAALVYHKHLKRLFLIDLNSTHGTFLGHIRLephkPQQVPID----STMSFGASTRVYT 96
Cdd:cd22704    20 FVGR--EDCDLILQSRSVDKQHAVITYDQIDNEFKIKDLGSLNGTFVNDSRI----PEQTYITlklgDSIRFGYDTNVYR 93

                  ....
gi 1709635275  97 IREK 100
Cdd:cd22704    94 FEQL 97

forkhead associated (FHA) domain found in Sulfolobus Acidocaldarius FHA domain-containing protein ArnA and similar proteins; ArnA is an FHA domain-containing protein from Sulfolobus acidocaldarius that was shown to strongly interact with ArnB, a von Willebrand domain-containing protein. They act synergistically and negatively to modulate motility. ArnA is involved in regulating archaella expression in S. acidocaldarius. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275   6 LVSLQKLIIDEKKFYL------FGRNPdICDFTIDHQSCSRVHAaLVYHKHlKRLFLIDLNSTHGTFLGHIRlEPHKPQQ 79
Cdd:cd22680     3 LEILSSPNLTGKKFPFdfssvsIGRDP-ENVIVIPDPFVSRNHA-RITVDS-NEIYIEDLGSTNGTFVNDFK-RIKGPAK 78

                          90
                  ....*....|....*
gi 1709635275  80 VPIDSTMSFGASTRV 94
Cdd:cd22680    79 LHPNDIIKLGRTTVL 93

first forkhead associated (FHA) domain found in Mycobacterium tuberculosis ABC transporter ATP-binding/permease protein Rv1747 and similar proteins; Rv1747 is a putative ATP-binding cassette (ABC) transporter involved in the translocation of an unknown substrate across the membrane. It is required for normal virulent infection by M. tuberculosis. Rv1747 has a cytoplasmic regulatory module consisting of two pThr-interacting forkhead-associated (FHA) domains connected by a conformationally disordered linker with two phospho-acceptor threonines (pThr). Recruitment and phosphorylation of Rv1747 depend on the interaction between its two non-redundant FHA domains and the autophosphorylated form of serine/threonine protein kinase PknF. This model corresponds to the first FHA domain. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1709635275  20 YLFGRNPDiCDFTIDHQSCSRVHAALvyHKHLKRLFLIDLNSTHGTFLGHIRLephkpQQVPIDS 84
Cdd:cd22694    18 VRIGRDPD-ADVRLDDPRVSRRHALL--EFDGDGWVYTDLGSRNGTYLNGRRV-----QQVKLSD 74

forkhead associated (FHA) domain found in Saccharomyces cerevisiae DNA damage response protein kinase DUN1 and similar proteins; DUN1 is a protein kinase that controls the DNA damage response in yeast. It phosphorylates SML1 on serine residues and cooperates with the PAN deadenylation complex in the regulation of RAD5 mRNA levels and cell survival in response to replicational stress. It contains an FHA domain, which is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1709635275  10 QKLIIDEKKFYLFGRNPDiCDFTIDHQSCSRVHAALvyHKHLKRLFLIDLNSTHGTFLGHIRLephKPQQVPIDST--MS 87
Cdd:cd22683    13 QKISITNRNVTTIGRSRS-CDLVLSDPSISRFHAEL--RLEQNGINVIDNNSANGTFINGKRI---KGKTYILKNGdiIV 86

                  ..
gi 1709635275  88 FG 89
Cdd:cd22683    87 FG 88

forkhead associated (FHA) domain found in Saccharomyces cerevisiae pre-mRNA leakage protein 1 (PML1) and similar proteins; PML1 is an FHA domain-containing protein required for efficient splicing and pre-mRNA nuclear retention. It is a component of the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3, and PML1. It contains an FHA domain, which is a small phosphopeptide recognition module.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1709635275  16 EKKFYLFGR------NPDICDFTIDHQSCSRVHAALVYHK---HLKrLFLIDLNSTHGTFL 67
Cdd:cd22681    38 SPSCYLIGRekgestEIVVADIGIPEETCSKQHCVIQFRNvkgILK-PYIMDLDSSNGTCL 97