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Conserved domains on  [gi|1728608609|gb|TXU37641|]
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glycine cleavage system protein H [Escherichia coli]

Protein Classification

glycine cleavage system protein H( domain architecture ID 10785558)

glycine cleavage system protein H plays a role in the degradation of glycine by shuttling the methylamine group of glycine from P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase).

EC:  1.4.1.27
Gene Ontology:  GO:0019464|GO:0009249|GO:0005960
PubMed:  20375021|18941301

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
2-129 5.89e-77

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


:

Pssm-ID: 440275  Cd Length: 128  Bit Score: 223.46  E-value: 5.89e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609   2 SNIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAV 81
Cdd:COG0509     1 MEIPDDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEV 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1728608609  82 NDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE 129
Cdd:COG0509    81 NEALEDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEEE 128
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
2-129 5.89e-77

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 223.46  E-value: 5.89e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609   2 SNIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAV 81
Cdd:COG0509     1 MEIPDDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEV 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1728608609  82 NDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE 129
Cdd:COG0509    81 NEALEDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEEE 128
PRK01202 PRK01202
glycine cleavage system protein GcvH;
1-128 1.24e-74

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 217.33  E-value: 1.24e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609   1 MSNIPAELKYSKEHEWLRKEaDGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVA 80
Cdd:PRK01202    1 MMNIPADLKYTKSHEWVRVE-GDTATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVE 79
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1728608609  81 VNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLED 128
Cdd:PRK01202   80 VNEALEDSPELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIEE 127
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
3-129 3.00e-65

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 193.90  E-value: 3.00e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609   3 NIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVN 82
Cdd:TIGR00527   1 QGPQDLRYSSEHEWVRVEGDKTATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVN 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1728608609  83 DALSDSPELVNSEPYAGGWIFKIKASD-ESELESLLDATAYEALLEDE 129
Cdd:TIGR00527  81 TALEDSPELVNEDPYGDGWLIKVKLSDgESEVEGLMSAEQYEATLEHD 128
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
8-129 6.88e-59

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 177.52  E-value: 6.88e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609   8 LKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSD 87
Cdd:pfam01597   1 LYYTKEHEWVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1728608609  88 SPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE 129
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKEI 122
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
10-105 9.78e-52

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 158.47  E-value: 9.78e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609  10 YSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSP 89
Cdd:cd06848     1 YTKDHEWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80
                          90
                  ....*....|....*.
gi 1728608609  90 ELVNSEPYAGGWIFKI 105
Cdd:cd06848    81 ELINSDPYGEGWLVKI 96
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
2-129 5.89e-77

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 223.46  E-value: 5.89e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609   2 SNIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAV 81
Cdd:COG0509     1 MEIPDDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEV 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1728608609  82 NDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE 129
Cdd:COG0509    81 NEALEDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEEE 128
PRK01202 PRK01202
glycine cleavage system protein GcvH;
1-128 1.24e-74

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 217.33  E-value: 1.24e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609   1 MSNIPAELKYSKEHEWLRKEaDGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVA 80
Cdd:PRK01202    1 MMNIPADLKYTKSHEWVRVE-GDTATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVE 79
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1728608609  81 VNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLED 128
Cdd:PRK01202   80 VNEALEDSPELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIEE 127
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
3-129 3.00e-65

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 193.90  E-value: 3.00e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609   3 NIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVN 82
Cdd:TIGR00527   1 QGPQDLRYSSEHEWVRVEGDKTATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVN 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1728608609  83 DALSDSPELVNSEPYAGGWIFKIKASD-ESELESLLDATAYEALLEDE 129
Cdd:TIGR00527  81 TALEDSPELVNEDPYGDGWLIKVKLSDgESEVEGLMSAEQYEATLEHD 128
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
8-129 6.88e-59

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 177.52  E-value: 6.88e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609   8 LKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSD 87
Cdd:pfam01597   1 LYYTKEHEWVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1728608609  88 SPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE 129
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKEI 122
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
1-126 6.45e-57

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 173.26  E-value: 6.45e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609   1 MSNIPAELKYSKE-HEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIV 79
Cdd:PRK13380    6 GCEIPSELRYTIKnHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKVEKGKPVATLESGKWAGPVPAPLTGEVV 85
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1728608609  80 AVNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALL 126
Cdd:PRK13380   86 EVNEALEDSPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLL 132
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
10-105 9.78e-52

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 158.47  E-value: 9.78e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609  10 YSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSP 89
Cdd:cd06848     1 YTKDHEWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80
                          90
                  ....*....|....*.
gi 1728608609  90 ELVNSEPYAGGWIFKI 105
Cdd:cd06848    81 ELINSDPYGEGWLVKI 96
PRK00624 PRK00624
glycine cleavage system protein H; Provisional
10-116 6.89e-19

glycine cleavage system protein H; Provisional


Pssm-ID: 167014  Cd Length: 114  Bit Score: 76.02  E-value: 6.89e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609  10 YSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSP 89
Cdd:PRK00624    4 YSDYHVWIEPIHSRIVRLGLTSKMQENLGNILHIDLPSVGSFCKEGEVLVILESSKSAIEVLSPVSGEVIEVNTALEDDI 83
                          90       100
                  ....*....|....*....|....*..
gi 1728608609  90 ELVNSEPYAGGWIFKIKASDESELESL 116
Cdd:PRK00624   84 QPINNAPESEGWFVVVQLDEDFDSENL 110
Biotinyl_lipoyl_domains cd06663
Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the ...
26-105 1.34e-12

Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases, and the H-protein of the glycine cleavage system (GCS). These domains transport CO2, acyl, or methylamine, respectively, between components of the complex/protein via a biotinyl or lipoyl group, which is covalently attached to a highly conserved lysine residue.


Pssm-ID: 133456 [Multi-domain]  Cd Length: 73  Bit Score: 58.61  E-value: 1.34e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1728608609  26 TVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDspelvnsEPYAGGWIFKI 105
Cdd:cd06663     1 TILIPDLAQHLGDGTVVKWLKKVGDKVKKGDVLAEIEAMKATSDVEAPKSGTVKKVLVKEGT-------KVEGDTPLVKI 73
PRK09282 PRK09282
pyruvate carboxylase subunit B; Validated
47-81 1.08e-03

pyruvate carboxylase subunit B; Validated


Pssm-ID: 236449 [Multi-domain]  Cd Length: 592  Bit Score: 37.51  E-value: 1.08e-03
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 1728608609  47 EVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAV 81
Cdd:PRK09282  539 KEGDKVKAGDTVLVLEAMKMENEIQAPVDGTVKEI 573
biotinyl_domain cd06850
The biotinyl-domain or biotin carboxyl carrier protein (BCCP) domain is present in all ...
41-82 8.86e-03

The biotinyl-domain or biotin carboxyl carrier protein (BCCP) domain is present in all biotin-dependent enzymes, such as acetyl-CoA carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, geranyl-CoA carboxylase, oxaloacetate decarboxylase, methylmalonyl-CoA decarboxylase, transcarboxylase and urea amidolyase. This domain functions in transferring CO2 from one subsite to another, allowing carboxylation, decarboxylation, or transcarboxylation. During this process, biotin is covalently attached to a specific lysine.


Pssm-ID: 133459 [Multi-domain]  Cd Length: 67  Bit Score: 32.77  E-value: 8.86e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1728608609  41 VFVdlpEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVN 82
Cdd:cd06850    13 VLV---KEGDKVEAGQPLAVLEAMKMENEVTAPVAGVVKEIL 51
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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