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formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme [Enterobacter roggenkampii]
Protein Classification
List of domain hits
Name
Accession
Description
Interval
E-value
PFL2-3 super family
cl36968 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
TIGR01774 :Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1349.57
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 8 TLSE RI KAH K T AL VHI V k PPVCT ERA QH YTE MYQ Q HMDK P IPV RRA L ALAH H L AKRT I W I KHD ELI I GN QASEV RAA P IF 87
Cdd:TIGR01774 1 MCMD RI EKL K K AL SKP V - ARLSV ERA RL YTE SMK Q TEGE P MII RRA K ALAH V L ENIP I Q I LDS ELI V GN MLPNP RAA I IF 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 88 PE YTVS WI E KE I D D L AD RP GAG FAVSEE N KRVL H - EI C P W W RGQ T VQ D RCY G MF TDE Q K G L LE T G I IKAE G n MTS G DA H L 166
Cdd:TIGR01774 80 PE GVGL WI I KE L D S L PN RP TNR FAVSEE D KRVL R e EI A P Y W QRK T IE D FAF G LM TDE M K I L YT T S I FVLT G - EIA G IS H V 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 167 AVN F P LV L EK GL DG L RAKVAE R RSRINLTVLEDLHGDQ F LK A ID IVLEAV SL HI K R F A D LA R EMA ST ET RV SRR D ELL AM 246
Cdd:TIGR01774 159 AVN Y P YL L RR GL RW L LEESER R IRALEESGVYEGEKYS F YQ A AK IVLEAV IN HI L R Y A K LA E EMA AS ET GE SRR E ELL KI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 247 AE N C DVI A H E P P K TFWQA L QL CYFI Q L ILQ I ESN GH S V S FA R M DQYLYP Y Y RR D VELNQ s L DRE H A I E L L H S C W L K LL E V 326
Cdd:TIGR01774 239 AE I C RKV A A E K P Q TFWQA V QL VWLV Q S ILQ Q ESN EQ S I S MG R I DQYLYP F Y KK D IGEGR - I DRE L A F E I L A S L W I K TN E I 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 327 NKI RS G S HSKAS AG S P LY Q N VTIGG Q klv NGEPM DAVN P LSY AI LE SCG RLR ST QPNL S VR YH AG MSND FL DACVQV IR C 406
Cdd:TIGR01774 318 VPA RS S S LEQYF AG Q P TN Q A VTIGG C --- DIYGN DAVN E LSY LM LE VTD RLR LR QPNL H VR IN AG SPES FL KRLAEA IR S 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 407 G F G M PA FN NDE I V I P E F IKL GV ERD DA Y DY AAI GC I E T A VG G KW g Y RCTGMSF IN F A R VM LA AL EG G R D ATS G KV F LPQE 486
Cdd:TIGR01774 395 G C G N PA LF NDE A V V P A F KNA GV DDR DA L DY TTD GC V E I A PF G NS - Y TSSDAAL IN V A K VM EY AL NE G V D LQF G YE F GAKT 473
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 487 KAL sagnf DNF E EVMAAWDT QI RY Y TRKSI E IEY V V D TMLE E NVHDI L C S AL V D DC I E RAKS I KQ GGA K Y DWVSGLQ VGI 566
Cdd:TIGR01774 474 EKP ----- KFL E DLLEKLRE QI SH Y VKLVV E GSN V L D YANA E VKPTP L L S LC V E DC F E KGVD I SR GGA R Y NFTGIQA VGI 548
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 567 ANLG N SL A A V K KL VFEQG VIGQQQ L AA AL ADD F D GLTHE QL R Q RLIN GA PKYGNDDD S VD MLLTRAYQT Y I EE LKQ Y H NP 646
Cdd:TIGR01774 549 ANLG D SL V A I K GA VFEQG AYSMDD L VE AL RKN F V GLTHE EK R H RLIN QS PKYGNDDD A VD KYTAMVLEW Y C EE VNR Y R NP 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 647 R ygrgpi GG NYY AG TSSI SANVPFG AA T M ATPDGRKA HT PLA E G A SP AS G T D HL GPTAVI G SV G KL PT E AILG G V LLN Q K 726
Cdd:TIGR01774 629 R ------ GG KFA AG CYPM SANVPFG FF T S ATPDGRKA GE PLA P G V SP ST G M D RE GPTAVI N SV S KL SY E NLPN G A LLN I K 702
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 727 L N P S TLE NDS D R QKL MVL LR TF F EVH K GW HIQ Y NIV SRE TL LE A KKH P DQ YR D LVVRVAGYSAFF TA LS PDA QDDII A RT 806
Cdd:TIGR01774 703 L S P A TLE EEG D K QKL IEA LR KS F MEL K VM HIQ F NIV KED TL RK A QQE P EK YR W LVVRVAGYSAFF VE LS RPV QDDII R RT 782
....
gi 1730594474 807 E H T L 810
Cdd:TIGR01774 783 S H R L 786
Name
Accession
Description
Interval
E-value
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1349.57
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 8 TLSE RI KAH K T AL VHI V k PPVCT ERA QH YTE MYQ Q HMDK P IPV RRA L ALAH H L AKRT I W I KHD ELI I GN QASEV RAA P IF 87
Cdd:TIGR01774 1 MCMD RI EKL K K AL SKP V - ARLSV ERA RL YTE SMK Q TEGE P MII RRA K ALAH V L ENIP I Q I LDS ELI V GN MLPNP RAA I IF 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 88 PE YTVS WI E KE I D D L AD RP GAG FAVSEE N KRVL H - EI C P W W RGQ T VQ D RCY G MF TDE Q K G L LE T G I IKAE G n MTS G DA H L 166
Cdd:TIGR01774 80 PE GVGL WI I KE L D S L PN RP TNR FAVSEE D KRVL R e EI A P Y W QRK T IE D FAF G LM TDE M K I L YT T S I FVLT G - EIA G IS H V 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 167 AVN F P LV L EK GL DG L RAKVAE R RSRINLTVLEDLHGDQ F LK A ID IVLEAV SL HI K R F A D LA R EMA ST ET RV SRR D ELL AM 246
Cdd:TIGR01774 159 AVN Y P YL L RR GL RW L LEESER R IRALEESGVYEGEKYS F YQ A AK IVLEAV IN HI L R Y A K LA E EMA AS ET GE SRR E ELL KI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 247 AE N C DVI A H E P P K TFWQA L QL CYFI Q L ILQ I ESN GH S V S FA R M DQYLYP Y Y RR D VELNQ s L DRE H A I E L L H S C W L K LL E V 326
Cdd:TIGR01774 239 AE I C RKV A A E K P Q TFWQA V QL VWLV Q S ILQ Q ESN EQ S I S MG R I DQYLYP F Y KK D IGEGR - I DRE L A F E I L A S L W I K TN E I 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 327 NKI RS G S HSKAS AG S P LY Q N VTIGG Q klv NGEPM DAVN P LSY AI LE SCG RLR ST QPNL S VR YH AG MSND FL DACVQV IR C 406
Cdd:TIGR01774 318 VPA RS S S LEQYF AG Q P TN Q A VTIGG C --- DIYGN DAVN E LSY LM LE VTD RLR LR QPNL H VR IN AG SPES FL KRLAEA IR S 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 407 G F G M PA FN NDE I V I P E F IKL GV ERD DA Y DY AAI GC I E T A VG G KW g Y RCTGMSF IN F A R VM LA AL EG G R D ATS G KV F LPQE 486
Cdd:TIGR01774 395 G C G N PA LF NDE A V V P A F KNA GV DDR DA L DY TTD GC V E I A PF G NS - Y TSSDAAL IN V A K VM EY AL NE G V D LQF G YE F GAKT 473
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 487 KAL sagnf DNF E EVMAAWDT QI RY Y TRKSI E IEY V V D TMLE E NVHDI L C S AL V D DC I E RAKS I KQ GGA K Y DWVSGLQ VGI 566
Cdd:TIGR01774 474 EKP ----- KFL E DLLEKLRE QI SH Y VKLVV E GSN V L D YANA E VKPTP L L S LC V E DC F E KGVD I SR GGA R Y NFTGIQA VGI 548
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 567 ANLG N SL A A V K KL VFEQG VIGQQQ L AA AL ADD F D GLTHE QL R Q RLIN GA PKYGNDDD S VD MLLTRAYQT Y I EE LKQ Y H NP 646
Cdd:TIGR01774 549 ANLG D SL V A I K GA VFEQG AYSMDD L VE AL RKN F V GLTHE EK R H RLIN QS PKYGNDDD A VD KYTAMVLEW Y C EE VNR Y R NP 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 647 R ygrgpi GG NYY AG TSSI SANVPFG AA T M ATPDGRKA HT PLA E G A SP AS G T D HL GPTAVI G SV G KL PT E AILG G V LLN Q K 726
Cdd:TIGR01774 629 R ------ GG KFA AG CYPM SANVPFG FF T S ATPDGRKA GE PLA P G V SP ST G M D RE GPTAVI N SV S KL SY E NLPN G A LLN I K 702
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 727 L N P S TLE NDS D R QKL MVL LR TF F EVH K GW HIQ Y NIV SRE TL LE A KKH P DQ YR D LVVRVAGYSAFF TA LS PDA QDDII A RT 806
Cdd:TIGR01774 703 L S P A TLE EEG D K QKL IEA LR KS F MEL K VM HIQ F NIV KED TL RK A QQE P EK YR W LVVRVAGYSAFF VE LS RPV QDDII R RT 782
....
gi 1730594474 807 E H T L 810
Cdd:TIGR01774 783 S H R L 786
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
10-807
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1160.88
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 10 S ERIK AH K TALVHI v KP PVCT ERA QH YTE M Y QQHMD KP IPV RRA L AL A H H L AKR TI W I KH DELI I GN QASEV RA A P I FPE 89
Cdd:cd01677 1 T ERIK RL K AKILTA - KP SISI ERA RL YTE S Y KETEG KP VII RRA K AL K H I L ENA TI Y I QD DELI V GN RGGKP RA V P V FPE 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 90 YT V S W I E K E I DDL AD RPG AG F AV SE EN K RV L H EI C P W W R G Q T VQ DRC YGM F TD E QKGLLET G IIKAEGNMT SG DA H L AV N 169
Cdd:cd01677 80 LS V H W V E D E L DDL PK RPG DP F VI SE DK K EY L E EI F P Y W K G K T LR DRC FKY F PE E TLIAMAA G VFTEFMYFF SG PG H V AV D 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 170 F P L VLEKGLDGL RAKVA E RRSRIN LT VL ED LHGDQ F LK A ID IV L EAV SLHI KR F A D LA R E M A ST ET RVS R RD ELL AM AE N 249
Cdd:cd01677 160 Y P K VLEKGLDGL IEEAK E AIEALD LT GP ED IDKIY F YQ A MI IV C EAV ITYA KR Y A E LA K E L A AK ET DPK R KA ELL EI AE I 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 250 C DVIAHE PP K TFW Q ALQ LCY FI Q LILQIESNGHS V S FA R M DQYLYP Y Y RR D V E LN q S L D RE H AIELL HSC W L K LL E V NK I 329
Cdd:cd01677 240 C RRVPAH PP R TFW E ALQ SFW FI H LILQIESNGHS I S PG R F DQYLYP F Y KQ D I E EG - R L T RE G AIELL ECL W I K IN E I NK V 318
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 330 RSG SHS K AS AG SPLY QN V TIGGQ KL v N G E pm DA V N P LSY A ILE SCG R L R ST QP N L S VRYHA GMSND FL DACVQ VIR C G F G 409
Cdd:cd01677 319 RSG ASA K YF AG YNTF QN L TIGGQ TE - D G S -- DA T N E LSY L ILE ATR R V R LP QP S L T VRYHA KSPDK FL KKAAE VIR L G L G 395
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 410 M PAF N NDE I VIP EFIKL GV ERD DA Y DY AA IGC I ET AVG G k WG YR C TG MSF IN F A R V MLAA L EG G R D AT SGK VFL P Q ek AL 489
Cdd:cd01677 396 Y PAF F NDE V VIP ALLRK GV SLE DA R DY GL IGC V ET GAP G - RK YR W TG TGY IN L A K V LEIT L NN G K D PR SGK QVG P E -- TG 472
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 490 S A GN F DN FEE VMA A WDT Q I R YYTRK S IEIEYVV D TMLE E NVHDILC SALVDDCIE RA K S I KQ GGA K Y DWVSGLQ VGIA N L 569
Cdd:cd01677 473 D A TD F KT FEE LWE A FKK Q L R HFIKL S VRANNIS D IAHA E VAPAPFL SALVDDCIE KG K D I NA GGA R Y NFGGIQG VGIA T L 552
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 570 G N SLAA V KKLVFE QGVIGQQQ L AA AL ADD F DG lt HEQL R Q RL I N G APKYGNDDD SV D MLLT R A Y QT Y IE E LKQ Y H NPR yg 649
Cdd:cd01677 553 G D SLAA I KKLVFE EKKLTMEE L LE AL KAN F AE -- GYEE R R RL L N N APKYGNDDD YA D NIAR R V Y EW Y CK E VEK Y Q NPR -- 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 650 rgpi GG NY Y A GT S S I SANVPFG AA T M ATPDGR K A H TPL AE G A SP AS GTD HL GPTAVI G SV G KL PTEA I L GG V LLNQK LN P 729
Cdd:cd01677 629 ---- GG KF Y P GT Y S V SANVPFG SV T G ATPDGR L A G TPL SD G V SP SQ GTD KK GPTAVI K SV S KL DHFN I S GG T LLNQK FS P 704
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1730594474 730 STLE NDSDRQ KL MV L L RT F F EV h K G W HIQ Y N I VS R ETL LE A K KHP DQ YRDL V VRVAGYSA F F TA LS PDA QD D IIARTE 807
Cdd:cd01677 705 STLE GEEGLK KL AA L I RT Y F DL - G G H HIQ F N V VS A ETL RD A Q KHP EK YRDL I VRVAGYSA Y F VE LS KEV QD E IIARTE 781
PflD
COG1882 Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
5-810
0e+00
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain]
Cd Length: 789
Bit Score: 1071.70
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 5 N L NTLS ER I K AHKTA L VH i V KP PVCT ERA QHY TE M Y QQHMDK P IPV RRA L A LA H H L AKRT I W IK H DELI I G N Q ASEV R AA 84
Cdd:COG1882 3 F L AGPT ER T K RLREK L LE - A KP LIDI ERA RLF TE S Y KETEGL P VII RRA K A FS H I L EHKP I Y IK D DELI V G L Q TDKP R KR 81
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 85 PIFPE YTVS W I E K E I D D L AD RP GA GF AV S E E N K RVLH EI C P W W R G Q T VQ D RCYGMFTD E QKGLLET GII K a EGNMTS G DA 164
Cdd:COG1882 82 PIFPE GGIR W V E D E L D A L PT RP QD GF EI S P E D K EIFR EI A P Y W K G K T HN D GVFDAYPE E IRKARKA GII T - GLPDAY G RG 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 165 H LAVNFPL VL EK GLDGL RAKVA E RRSRIN LT VL ED LHGDQ F L KA ID IV L EAV SLHIK R F A D LARE M A ST ET RVS R RD ELL 244
Cdd:COG1882 161 H IIGDYRR VL LY GLDGL IEEAK E KLAELD LT DP ED IEKID F Y KA MI IV C EAV IRLAE R Y A E LARE L A EK ET DPK R KA ELL 240
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 245 AM AE N C DVIAHE P PK TFW Q A L Q LCY F IQ L ILQI E S NG HSV S FA R M DQYLYPYY R RD V E l NQS L DR E H A I ELL HSC W L KL L 324
Cdd:COG1882 241 EI AE I C GFVPAN P AR TFW E A V Q WVW F VY L AAIK E Q NG AAM S LG R F DQYLYPYY E RD L E - EGR L TE E E A Q ELL DCF W I KL R 319
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 325 EV NKI R SGSHSKAS AG S P LYQNV TIGG QK l VN G E pm DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ VI 404
Cdd:COG1882 320 EV RFL R TPEYAELF AG Y P TWVTL TIGG MT - PD G R -- DAVN E LSY L ILE TLRN L PLPE PNL T VR WSEKLPEG FL KKAAE VI 396
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 405 RC G F G M P AFN ND EIV IP EFIKL GV ERD DA Y DY AAI GC I E TA V G GK W g YRCT G MSF IN F A RVMLA AL EG G R D ATS GK VFL P 484
Cdd:COG1882 397 SI G T G S P QYE ND DLM IP MLLNK GV TLE DA R DY GIA GC V E PM V P GK Q - MQFF G AGR IN L A KALEY AL NN G V D EKT GK QVG P 475
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 485 QEKALS ag N F DNFE EVM A A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV 564
Cdd:COG1882 476 ETGDPT -- D F LTYD EVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DLNE GGA R Y NFGAIGIA 553
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 565 G IANLGN SL A A V KKLVF EQGVIGQQQ L AA ALA DD F D G lt H E Q LRQ R L I N g APKYGNDDD S VD MLLTRAYQ T YIE E LKQ Y H 644
Cdd:COG1882 554 G LSVVAD SL S A I KKLVF DKKKVTMDE L LE ALA AN F E G -- Y E E LRQ L L L N - APKYGNDDD Y VD EIAVELVE T FMD E IRK Y K 630
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 645 NP R ygrgpi GG N Y YAGTSS I SA NVP F G AA T M ATPDGRKA HT PLA E GASP AS G T D HL GPTAV IG SV G KLP T E AILG G V LLN 724
Cdd:COG1882 631 TY R ------ GG T Y TLSILT I TS NVP Y G KK T G ATPDGRKA GE PLA D GASP MH G R D KN GPTAV LK SV A KLP Y E KATD G I LLN 704
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 725 QK LN PS T L ENDSDRQK L MV LLRT F F E v HK G W HIQ Y N I V S RETLL E A K KHP DQ Y R DL V VRVAGYSA F F TA LS PDA QDDIIA 804
Cdd:COG1882 705 QK FS PS A L GGEEGIEN L VS LLRT Y F D - LG G H HIQ F N V V D RETLL D A Q KHP EK Y P DL T VRVAGYSA Y F VE LS KEQ QDDIIA 783
....*.
gi 1730594474 805 RTEH TL 810
Cdd:COG1882 784 RTEH EF 789
PFL-like
pfam02901 Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
11-671
0e+00
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048
Cd Length: 647
Bit Score: 741.76
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 11 ERI K AHK T al VHIVK P PVCT ERA QHY TE M Y QQHMDK - P IPV RRA L AL AHH L AKRTIW I KH DELI I G N Q ASEV R AAP I F PE 89
Cdd:pfam02901 6 DVL K ENY T -- LYTGD P SLSW ERA RLL TE S Y KETEGV l P VDI RRA K AL KKI L SHLPGY I RD DELI V G L Q TDKP R KRA I Y PE 83
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 90 YTVS W I E K E I D D L AD RP GA GF AV SEE N K RVLH EI C P W W R G Q T VQDRCYGMF T D E Q K GLL E T GI IK a EGNMTS G DA H LAVN 169
Cdd:pfam02901 84 GGIR W V E D E L D Y L NT RP QD GF EI SEE D K KIFR EI F P Y W K G K T HNEGVFDAY T P E M K AAR E S GI FT - GLPDAY G RG H IIGD 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 170 FPL VL EK GLDGL RAKVA E RRSRIN l T VL ED LHGDQ F L KA ID I VLE AV SLHIK R F A D LA R E M A ST ET RVS R RD ELL AM AE N 249
Cdd:pfam02901 163 YRR VL LY GLDGL IEEKE E KLAKLD - T DP ED IEKIE F Y KA MI I SCD AV IEYAE R Y A R LA E E L A EQ ET DPK R KA ELL EI AE I 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 250 C DVIAHE P PK TF WQ A L Q LCY F IQ L ILQI E S NG HSV S FA R M DQYLYPYY R RD V E l NQS L DR E H A I EL LHSC W L KL L EV NKI 329
Cdd:pfam02901 242 C GRVPAR P AE TF QE A I Q WFW F VY L AAVK E Q NG AAM S LG R L DQYLYPYY E RD L E - EGR L TE E E A Q EL IDCF W I KL R EV RFL 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 330 R SGSHS K AS AG SPLY QN V TIGGQ KL v N G E pm DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ V I R C G F G 409
Cdd:pfam02901 321 R TPEYN K LF AG YDPF QN L TIGGQ GR - D G R -- DAVN K LSY L ILE ALDN L PLPE PNL T VR WSKKLPEE FL KKAAE V S R K G T G 397
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 410 M P AFN ND EIV IP EFIKL GV ERD DA Y DY AAI GC I E TAVG GK - WGYRC tgm SF IN F A RVMLA AL E GGRD ATS GK VFL P QEKA 488
Cdd:pfam02901 398 S P QYE ND DVM IP ALLNR GV SLE DA R DY GIA GC V E PMKP GK e MQFFG --- AR IN L A KALEY AL N GGRD ELT GK QVG P KTGP 474
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 489 LS ag N F DN FEEVM A A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV G I AN 568
Cdd:pfam02901 475 VT -- E F LS FEEVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DVGI GGA R Y NFSGPQGA G L AN 552
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 569 LGN SL A A V KKLVF EQG V IGQQQ L AA ALA D DF D G lt H E Q LRQ R L I N G APKYGNDDD S VD MLLTRAYQ T YIE E LKQ Y H N P R y 648
Cdd:pfam02901 553 VAD SL S A I KKLVF DDK V YTLRE L ED ALA A DF E G -- E E E LRQ D L L N D APKYGNDDD R VD DIAVEVVE T FMD E VRK Y K N Y R - 629
650 660
....*....|....*....|...
gi 1730594474 649 grgpi GG NYYAGTSS I SA NVP F G 671
Cdd:pfam02901 630 ----- GG KFTPSLLT I TS NVP Y G 647
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
9-810
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 582.16
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 9 LSE RI KAH KTAL VHIVKP p VCT ERA QH YT EMYQ Q HMDK P IPV RRA L A L A HH L AKRT I W I KHD ELI I GN QASEV RA APIF P 88
Cdd:PRK09983 1 MTN RI SRL KTAL FANTRE - ISL ERA LL YT ASHR Q TEGE P VIL RRA K A T A YI L EHVE I S I RDE ELI A GN RTVKP RA GIMS P 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 89 E YTVS W IE KE I D DLAD RP GAG FA V SEE N KR VLH E - IC P W W RGQTVQ D RCY G MF TDE Q K GLLE T G I IKA eg N M T - S G DA H L 166
Cdd:PRK09983 80 E MDPY W LL KE L D QFPT RP QDR FA I SEE D KR IYR E e LF P Y W EKRSMK D FIN G QM TDE V K AATS T Q I FSI -- N Q T d K G QG H I 157
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 167 AVNF P LV L EK GL DG L R A KVAER rsrinltv LEDLHGDQ F LK A IDIV LEA VSL HI K R F A D LA RE MA STE T RVS RR D ELL AM 246
Cdd:PRK09983 158 IIDY P RL L NH GL GE L V A QMQQH -------- CQQQPENH F YQ A ALLL LEA SQK HI L R Y A E LA ET MA ANC T DAQ RR E ELL TI 229
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 247 AE NCDVI A HEP P K TFWQA L QL CYFIQL ILQ I ESN GH S V S FA R M DQY LY P Y Y RRD ve L N Q SL D REHAI ELL H S C W L K LLEV 326
Cdd:PRK09983 230 AE ISRHN A QHK P Q TFWQA C QL FWYMNI ILQ Y ESN AS S L S LG R F DQY ML P F Y QAS -- L T Q GE D PAFLK ELL E S L W V K CNDI 307
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 327 NKI RS G S HSKAS AG S P LYQNVTI GG qkl VNGEPMD AVN P LS YAI L ESCGRLRST QPNL S VR YH A GMSND FL DACVQV IR C 406
Cdd:PRK09983 308 VLL RS T S SARYF AG F P TGYTALL GG --- LTENGRS AVN V LS FLC L DAYQSVQLP QPNL G VR TN A LIDTP FL MKTAET IR L 384
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 407 G F G M P AFN NDE I V I P E F IKL GV ERD DA Y DY AAI GC I E TAVG G K w G Y RCTGMSFI N FAR VM LAA L --- EG GRDA T sgkvfl 483
Cdd:PRK09983 385 G T G I P QIF NDE V V V P A F LNR GV SLE DA R DY SVV GC V E LSIP G R - T Y GLHDIAMF N LLK VM EIC L hen EG NAAL T ------ 457
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 484 pqekalsagnfdn F E EVMAAWDTQ I RY Y TRKSI E IEYVV D TMLEENVHDI L C S ALVD DC I E RAKS I KQ GGA K Y DW v SG L Q 563
Cdd:PRK09983 458 ------------- Y E GLLEQIRAK I SH Y ITLMV E GSNIC D IGHRDWAPVP L L S SFIS DC L E KGRD I TD GGA R Y NF - SG V Q 523
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 564 - V GIANL GN SL A A V K KL VF E Q GVIGQQQ L AAA L ADD F DGLTH E QL R Q RLIN GAP KYGND D D S VD MLLTRAYQT Y IE E LKQ 642
Cdd:PRK09983 524 g I GIANL SD SL H A L K GM VF D Q QRLSFDE L LSV L KAN F ATPEG E KV R A RLIN RFE KYGND I D E VD NISAELLRH Y CK E VEK 603
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 643 Y H NPR ygrgpi GG NYYA G TSSI SA N VP F G AATM ATPDGR K A HTP LA E G A - SP AS G T D HL GPTAV IG SV G KL PTEAILG G V 721
Cdd:PRK09983 604 Y Q NPR ------ GG YFTP G SYTV SA H VP L G SVVG ATPDGR F A GEQ LA D G G l SP ML G Q D AQ GPTAV LK SV S KL DNTLLSN G T 677
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 722 LLN Q K LN P S TLE NDSDRQ KL MVL LR T F FEV h K GW HIQ Y N I V SRE TL L EA KKH P DQ Y RD LVVRVAGYSAFF TA LS PDA QDD 801
Cdd:PRK09983 678 LLN V K FT P A TLE GEAGLR KL ADF LR A F TQL - K LQ HIQ F N V V NAD TL R EA QQR P QD Y AG LVVRVAGYSAFF VE LS KEI QDD 756
....*....
gi 1730594474 802 II A RT E H T L 810
Cdd:PRK09983 757 II R RT A H Q L 765
Name
Accession
Description
Interval
E-value
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1349.57
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 8 TLSE RI KAH K T AL VHI V k PPVCT ERA QH YTE MYQ Q HMDK P IPV RRA L ALAH H L AKRT I W I KHD ELI I GN QASEV RAA P IF 87
Cdd:TIGR01774 1 MCMD RI EKL K K AL SKP V - ARLSV ERA RL YTE SMK Q TEGE P MII RRA K ALAH V L ENIP I Q I LDS ELI V GN MLPNP RAA I IF 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 88 PE YTVS WI E KE I D D L AD RP GAG FAVSEE N KRVL H - EI C P W W RGQ T VQ D RCY G MF TDE Q K G L LE T G I IKAE G n MTS G DA H L 166
Cdd:TIGR01774 80 PE GVGL WI I KE L D S L PN RP TNR FAVSEE D KRVL R e EI A P Y W QRK T IE D FAF G LM TDE M K I L YT T S I FVLT G - EIA G IS H V 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 167 AVN F P LV L EK GL DG L RAKVAE R RSRINLTVLEDLHGDQ F LK A ID IVLEAV SL HI K R F A D LA R EMA ST ET RV SRR D ELL AM 246
Cdd:TIGR01774 159 AVN Y P YL L RR GL RW L LEESER R IRALEESGVYEGEKYS F YQ A AK IVLEAV IN HI L R Y A K LA E EMA AS ET GE SRR E ELL KI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 247 AE N C DVI A H E P P K TFWQA L QL CYFI Q L ILQ I ESN GH S V S FA R M DQYLYP Y Y RR D VELNQ s L DRE H A I E L L H S C W L K LL E V 326
Cdd:TIGR01774 239 AE I C RKV A A E K P Q TFWQA V QL VWLV Q S ILQ Q ESN EQ S I S MG R I DQYLYP F Y KK D IGEGR - I DRE L A F E I L A S L W I K TN E I 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 327 NKI RS G S HSKAS AG S P LY Q N VTIGG Q klv NGEPM DAVN P LSY AI LE SCG RLR ST QPNL S VR YH AG MSND FL DACVQV IR C 406
Cdd:TIGR01774 318 VPA RS S S LEQYF AG Q P TN Q A VTIGG C --- DIYGN DAVN E LSY LM LE VTD RLR LR QPNL H VR IN AG SPES FL KRLAEA IR S 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 407 G F G M PA FN NDE I V I P E F IKL GV ERD DA Y DY AAI GC I E T A VG G KW g Y RCTGMSF IN F A R VM LA AL EG G R D ATS G KV F LPQE 486
Cdd:TIGR01774 395 G C G N PA LF NDE A V V P A F KNA GV DDR DA L DY TTD GC V E I A PF G NS - Y TSSDAAL IN V A K VM EY AL NE G V D LQF G YE F GAKT 473
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 487 KAL sagnf DNF E EVMAAWDT QI RY Y TRKSI E IEY V V D TMLE E NVHDI L C S AL V D DC I E RAKS I KQ GGA K Y DWVSGLQ VGI 566
Cdd:TIGR01774 474 EKP ----- KFL E DLLEKLRE QI SH Y VKLVV E GSN V L D YANA E VKPTP L L S LC V E DC F E KGVD I SR GGA R Y NFTGIQA VGI 548
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 567 ANLG N SL A A V K KL VFEQG VIGQQQ L AA AL ADD F D GLTHE QL R Q RLIN GA PKYGNDDD S VD MLLTRAYQT Y I EE LKQ Y H NP 646
Cdd:TIGR01774 549 ANLG D SL V A I K GA VFEQG AYSMDD L VE AL RKN F V GLTHE EK R H RLIN QS PKYGNDDD A VD KYTAMVLEW Y C EE VNR Y R NP 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 647 R ygrgpi GG NYY AG TSSI SANVPFG AA T M ATPDGRKA HT PLA E G A SP AS G T D HL GPTAVI G SV G KL PT E AILG G V LLN Q K 726
Cdd:TIGR01774 629 R ------ GG KFA AG CYPM SANVPFG FF T S ATPDGRKA GE PLA P G V SP ST G M D RE GPTAVI N SV S KL SY E NLPN G A LLN I K 702
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 727 L N P S TLE NDS D R QKL MVL LR TF F EVH K GW HIQ Y NIV SRE TL LE A KKH P DQ YR D LVVRVAGYSAFF TA LS PDA QDDII A RT 806
Cdd:TIGR01774 703 L S P A TLE EEG D K QKL IEA LR KS F MEL K VM HIQ F NIV KED TL RK A QQE P EK YR W LVVRVAGYSAFF VE LS RPV QDDII R RT 782
....
gi 1730594474 807 E H T L 810
Cdd:TIGR01774 783 S H R L 786
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
10-807
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1160.88
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 10 S ERIK AH K TALVHI v KP PVCT ERA QH YTE M Y QQHMD KP IPV RRA L AL A H H L AKR TI W I KH DELI I GN QASEV RA A P I FPE 89
Cdd:cd01677 1 T ERIK RL K AKILTA - KP SISI ERA RL YTE S Y KETEG KP VII RRA K AL K H I L ENA TI Y I QD DELI V GN RGGKP RA V P V FPE 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 90 YT V S W I E K E I DDL AD RPG AG F AV SE EN K RV L H EI C P W W R G Q T VQ DRC YGM F TD E QKGLLET G IIKAEGNMT SG DA H L AV N 169
Cdd:cd01677 80 LS V H W V E D E L DDL PK RPG DP F VI SE DK K EY L E EI F P Y W K G K T LR DRC FKY F PE E TLIAMAA G VFTEFMYFF SG PG H V AV D 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 170 F P L VLEKGLDGL RAKVA E RRSRIN LT VL ED LHGDQ F LK A ID IV L EAV SLHI KR F A D LA R E M A ST ET RVS R RD ELL AM AE N 249
Cdd:cd01677 160 Y P K VLEKGLDGL IEEAK E AIEALD LT GP ED IDKIY F YQ A MI IV C EAV ITYA KR Y A E LA K E L A AK ET DPK R KA ELL EI AE I 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 250 C DVIAHE PP K TFW Q ALQ LCY FI Q LILQIESNGHS V S FA R M DQYLYP Y Y RR D V E LN q S L D RE H AIELL HSC W L K LL E V NK I 329
Cdd:cd01677 240 C RRVPAH PP R TFW E ALQ SFW FI H LILQIESNGHS I S PG R F DQYLYP F Y KQ D I E EG - R L T RE G AIELL ECL W I K IN E I NK V 318
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 330 RSG SHS K AS AG SPLY QN V TIGGQ KL v N G E pm DA V N P LSY A ILE SCG R L R ST QP N L S VRYHA GMSND FL DACVQ VIR C G F G 409
Cdd:cd01677 319 RSG ASA K YF AG YNTF QN L TIGGQ TE - D G S -- DA T N E LSY L ILE ATR R V R LP QP S L T VRYHA KSPDK FL KKAAE VIR L G L G 395
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 410 M PAF N NDE I VIP EFIKL GV ERD DA Y DY AA IGC I ET AVG G k WG YR C TG MSF IN F A R V MLAA L EG G R D AT SGK VFL P Q ek AL 489
Cdd:cd01677 396 Y PAF F NDE V VIP ALLRK GV SLE DA R DY GL IGC V ET GAP G - RK YR W TG TGY IN L A K V LEIT L NN G K D PR SGK QVG P E -- TG 472
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 490 S A GN F DN FEE VMA A WDT Q I R YYTRK S IEIEYVV D TMLE E NVHDILC SALVDDCIE RA K S I KQ GGA K Y DWVSGLQ VGIA N L 569
Cdd:cd01677 473 D A TD F KT FEE LWE A FKK Q L R HFIKL S VRANNIS D IAHA E VAPAPFL SALVDDCIE KG K D I NA GGA R Y NFGGIQG VGIA T L 552
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 570 G N SLAA V KKLVFE QGVIGQQQ L AA AL ADD F DG lt HEQL R Q RL I N G APKYGNDDD SV D MLLT R A Y QT Y IE E LKQ Y H NPR yg 649
Cdd:cd01677 553 G D SLAA I KKLVFE EKKLTMEE L LE AL KAN F AE -- GYEE R R RL L N N APKYGNDDD YA D NIAR R V Y EW Y CK E VEK Y Q NPR -- 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 650 rgpi GG NY Y A GT S S I SANVPFG AA T M ATPDGR K A H TPL AE G A SP AS GTD HL GPTAVI G SV G KL PTEA I L GG V LLNQK LN P 729
Cdd:cd01677 629 ---- GG KF Y P GT Y S V SANVPFG SV T G ATPDGR L A G TPL SD G V SP SQ GTD KK GPTAVI K SV S KL DHFN I S GG T LLNQK FS P 704
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1730594474 730 STLE NDSDRQ KL MV L L RT F F EV h K G W HIQ Y N I VS R ETL LE A K KHP DQ YRDL V VRVAGYSA F F TA LS PDA QD D IIARTE 807
Cdd:cd01677 705 STLE GEEGLK KL AA L I RT Y F DL - G G H HIQ F N V VS A ETL RD A Q KHP EK YRDL I VRVAGYSA Y F VE LS KEV QD E IIARTE 781
PflD
COG1882 Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
5-810
0e+00
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain]
Cd Length: 789
Bit Score: 1071.70
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 5 N L NTLS ER I K AHKTA L VH i V KP PVCT ERA QHY TE M Y QQHMDK P IPV RRA L A LA H H L AKRT I W IK H DELI I G N Q ASEV R AA 84
Cdd:COG1882 3 F L AGPT ER T K RLREK L LE - A KP LIDI ERA RLF TE S Y KETEGL P VII RRA K A FS H I L EHKP I Y IK D DELI V G L Q TDKP R KR 81
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 85 PIFPE YTVS W I E K E I D D L AD RP GA GF AV S E E N K RVLH EI C P W W R G Q T VQ D RCYGMFTD E QKGLLET GII K a EGNMTS G DA 164
Cdd:COG1882 82 PIFPE GGIR W V E D E L D A L PT RP QD GF EI S P E D K EIFR EI A P Y W K G K T HN D GVFDAYPE E IRKARKA GII T - GLPDAY G RG 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 165 H LAVNFPL VL EK GLDGL RAKVA E RRSRIN LT VL ED LHGDQ F L KA ID IV L EAV SLHIK R F A D LARE M A ST ET RVS R RD ELL 244
Cdd:COG1882 161 H IIGDYRR VL LY GLDGL IEEAK E KLAELD LT DP ED IEKID F Y KA MI IV C EAV IRLAE R Y A E LARE L A EK ET DPK R KA ELL 240
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 245 AM AE N C DVIAHE P PK TFW Q A L Q LCY F IQ L ILQI E S NG HSV S FA R M DQYLYPYY R RD V E l NQS L DR E H A I ELL HSC W L KL L 324
Cdd:COG1882 241 EI AE I C GFVPAN P AR TFW E A V Q WVW F VY L AAIK E Q NG AAM S LG R F DQYLYPYY E RD L E - EGR L TE E E A Q ELL DCF W I KL R 319
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 325 EV NKI R SGSHSKAS AG S P LYQNV TIGG QK l VN G E pm DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ VI 404
Cdd:COG1882 320 EV RFL R TPEYAELF AG Y P TWVTL TIGG MT - PD G R -- DAVN E LSY L ILE TLRN L PLPE PNL T VR WSEKLPEG FL KKAAE VI 396
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 405 RC G F G M P AFN ND EIV IP EFIKL GV ERD DA Y DY AAI GC I E TA V G GK W g YRCT G MSF IN F A RVMLA AL EG G R D ATS GK VFL P 484
Cdd:COG1882 397 SI G T G S P QYE ND DLM IP MLLNK GV TLE DA R DY GIA GC V E PM V P GK Q - MQFF G AGR IN L A KALEY AL NN G V D EKT GK QVG P 475
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 485 QEKALS ag N F DNFE EVM A A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV 564
Cdd:COG1882 476 ETGDPT -- D F LTYD EVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DLNE GGA R Y NFGAIGIA 553
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 565 G IANLGN SL A A V KKLVF EQGVIGQQQ L AA ALA DD F D G lt H E Q LRQ R L I N g APKYGNDDD S VD MLLTRAYQ T YIE E LKQ Y H 644
Cdd:COG1882 554 G LSVVAD SL S A I KKLVF DKKKVTMDE L LE ALA AN F E G -- Y E E LRQ L L L N - APKYGNDDD Y VD EIAVELVE T FMD E IRK Y K 630
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 645 NP R ygrgpi GG N Y YAGTSS I SA NVP F G AA T M ATPDGRKA HT PLA E GASP AS G T D HL GPTAV IG SV G KLP T E AILG G V LLN 724
Cdd:COG1882 631 TY R ------ GG T Y TLSILT I TS NVP Y G KK T G ATPDGRKA GE PLA D GASP MH G R D KN GPTAV LK SV A KLP Y E KATD G I LLN 704
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 725 QK LN PS T L ENDSDRQK L MV LLRT F F E v HK G W HIQ Y N I V S RETLL E A K KHP DQ Y R DL V VRVAGYSA F F TA LS PDA QDDIIA 804
Cdd:COG1882 705 QK FS PS A L GGEEGIEN L VS LLRT Y F D - LG G H HIQ F N V V D RETLL D A Q KHP EK Y P DL T VRVAGYSA Y F VE LS KEQ QDDIIA 783
....*.
gi 1730594474 805 RTEH TL 810
Cdd:COG1882 784 RTEH EF 789
PFL-like
pfam02901 Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
11-671
0e+00
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048
Cd Length: 647
Bit Score: 741.76
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 11 ERI K AHK T al VHIVK P PVCT ERA QHY TE M Y QQHMDK - P IPV RRA L AL AHH L AKRTIW I KH DELI I G N Q ASEV R AAP I F PE 89
Cdd:pfam02901 6 DVL K ENY T -- LYTGD P SLSW ERA RLL TE S Y KETEGV l P VDI RRA K AL KKI L SHLPGY I RD DELI V G L Q TDKP R KRA I Y PE 83
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 90 YTVS W I E K E I D D L AD RP GA GF AV SEE N K RVLH EI C P W W R G Q T VQDRCYGMF T D E Q K GLL E T GI IK a EGNMTS G DA H LAVN 169
Cdd:pfam02901 84 GGIR W V E D E L D Y L NT RP QD GF EI SEE D K KIFR EI F P Y W K G K T HNEGVFDAY T P E M K AAR E S GI FT - GLPDAY G RG H IIGD 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 170 FPL VL EK GLDGL RAKVA E RRSRIN l T VL ED LHGDQ F L KA ID I VLE AV SLHIK R F A D LA R E M A ST ET RVS R RD ELL AM AE N 249
Cdd:pfam02901 163 YRR VL LY GLDGL IEEKE E KLAKLD - T DP ED IEKIE F Y KA MI I SCD AV IEYAE R Y A R LA E E L A EQ ET DPK R KA ELL EI AE I 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 250 C DVIAHE P PK TF WQ A L Q LCY F IQ L ILQI E S NG HSV S FA R M DQYLYPYY R RD V E l NQS L DR E H A I EL LHSC W L KL L EV NKI 329
Cdd:pfam02901 242 C GRVPAR P AE TF QE A I Q WFW F VY L AAVK E Q NG AAM S LG R L DQYLYPYY E RD L E - EGR L TE E E A Q EL IDCF W I KL R EV RFL 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 330 R SGSHS K AS AG SPLY QN V TIGGQ KL v N G E pm DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ V I R C G F G 409
Cdd:pfam02901 321 R TPEYN K LF AG YDPF QN L TIGGQ GR - D G R -- DAVN K LSY L ILE ALDN L PLPE PNL T VR WSKKLPEE FL KKAAE V S R K G T G 397
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 410 M P AFN ND EIV IP EFIKL GV ERD DA Y DY AAI GC I E TAVG GK - WGYRC tgm SF IN F A RVMLA AL E GGRD ATS GK VFL P QEKA 488
Cdd:pfam02901 398 S P QYE ND DVM IP ALLNR GV SLE DA R DY GIA GC V E PMKP GK e MQFFG --- AR IN L A KALEY AL N GGRD ELT GK QVG P KTGP 474
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 489 LS ag N F DN FEEVM A A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV G I AN 568
Cdd:pfam02901 475 VT -- E F LS FEEVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DVGI GGA R Y NFSGPQGA G L AN 552
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 569 LGN SL A A V KKLVF EQG V IGQQQ L AA ALA D DF D G lt H E Q LRQ R L I N G APKYGNDDD S VD MLLTRAYQ T YIE E LKQ Y H N P R y 648
Cdd:pfam02901 553 VAD SL S A I KKLVF DDK V YTLRE L ED ALA A DF E G -- E E E LRQ D L L N D APKYGNDDD R VD DIAVEVVE T FMD E VRK Y K N Y R - 629
650 660
....*....|....*....|...
gi 1730594474 649 grgpi GG NYYAGTSS I SA NVP F G 671
Cdd:pfam02901 630 ----- GG KFTPSLLT I TS NVP Y G 647
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
9-810
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 582.16
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 9 LSE RI KAH KTAL VHIVKP p VCT ERA QH YT EMYQ Q HMDK P IPV RRA L A L A HH L AKRT I W I KHD ELI I GN QASEV RA APIF P 88
Cdd:PRK09983 1 MTN RI SRL KTAL FANTRE - ISL ERA LL YT ASHR Q TEGE P VIL RRA K A T A YI L EHVE I S I RDE ELI A GN RTVKP RA GIMS P 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 89 E YTVS W IE KE I D DLAD RP GAG FA V SEE N KR VLH E - IC P W W RGQTVQ D RCY G MF TDE Q K GLLE T G I IKA eg N M T - S G DA H L 166
Cdd:PRK09983 80 E MDPY W LL KE L D QFPT RP QDR FA I SEE D KR IYR E e LF P Y W EKRSMK D FIN G QM TDE V K AATS T Q I FSI -- N Q T d K G QG H I 157
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 167 AVNF P LV L EK GL DG L R A KVAER rsrinltv LEDLHGDQ F LK A IDIV LEA VSL HI K R F A D LA RE MA STE T RVS RR D ELL AM 246
Cdd:PRK09983 158 IIDY P RL L NH GL GE L V A QMQQH -------- CQQQPENH F YQ A ALLL LEA SQK HI L R Y A E LA ET MA ANC T DAQ RR E ELL TI 229
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 247 AE NCDVI A HEP P K TFWQA L QL CYFIQL ILQ I ESN GH S V S FA R M DQY LY P Y Y RRD ve L N Q SL D REHAI ELL H S C W L K LLEV 326
Cdd:PRK09983 230 AE ISRHN A QHK P Q TFWQA C QL FWYMNI ILQ Y ESN AS S L S LG R F DQY ML P F Y QAS -- L T Q GE D PAFLK ELL E S L W V K CNDI 307
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 327 NKI RS G S HSKAS AG S P LYQNVTI GG qkl VNGEPMD AVN P LS YAI L ESCGRLRST QPNL S VR YH A GMSND FL DACVQV IR C 406
Cdd:PRK09983 308 VLL RS T S SARYF AG F P TGYTALL GG --- LTENGRS AVN V LS FLC L DAYQSVQLP QPNL G VR TN A LIDTP FL MKTAET IR L 384
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 407 G F G M P AFN NDE I V I P E F IKL GV ERD DA Y DY AAI GC I E TAVG G K w G Y RCTGMSFI N FAR VM LAA L --- EG GRDA T sgkvfl 483
Cdd:PRK09983 385 G T G I P QIF NDE V V V P A F LNR GV SLE DA R DY SVV GC V E LSIP G R - T Y GLHDIAMF N LLK VM EIC L hen EG NAAL T ------ 457
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 484 pqekalsagnfdn F E EVMAAWDTQ I RY Y TRKSI E IEYVV D TMLEENVHDI L C S ALVD DC I E RAKS I KQ GGA K Y DW v SG L Q 563
Cdd:PRK09983 458 ------------- Y E GLLEQIRAK I SH Y ITLMV E GSNIC D IGHRDWAPVP L L S SFIS DC L E KGRD I TD GGA R Y NF - SG V Q 523
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 564 - V GIANL GN SL A A V K KL VF E Q GVIGQQQ L AAA L ADD F DGLTH E QL R Q RLIN GAP KYGND D D S VD MLLTRAYQT Y IE E LKQ 642
Cdd:PRK09983 524 g I GIANL SD SL H A L K GM VF D Q QRLSFDE L LSV L KAN F ATPEG E KV R A RLIN RFE KYGND I D E VD NISAELLRH Y CK E VEK 603
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 643 Y H NPR ygrgpi GG NYYA G TSSI SA N VP F G AATM ATPDGR K A HTP LA E G A - SP AS G T D HL GPTAV IG SV G KL PTEAILG G V 721
Cdd:PRK09983 604 Y Q NPR ------ GG YFTP G SYTV SA H VP L G SVVG ATPDGR F A GEQ LA D G G l SP ML G Q D AQ GPTAV LK SV S KL DNTLLSN G T 677
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 722 LLN Q K LN P S TLE NDSDRQ KL MVL LR T F FEV h K GW HIQ Y N I V SRE TL L EA KKH P DQ Y RD LVVRVAGYSAFF TA LS PDA QDD 801
Cdd:PRK09983 678 LLN V K FT P A TLE GEAGLR KL ADF LR A F TQL - K LQ HIQ F N V V NAD TL R EA QQR P QD Y AG LVVRVAGYSAFF VE LS KEI QDD 756
....*....
gi 1730594474 802 II A RT E H T L 810
Cdd:PRK09983 757 II R RT A H Q L 765
choline_CutC
TIGR04394 choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and ...
9-806
3.98e-151
choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and benzylsuccinate synthases, are glycine radical enzymes that appear to act as choline TMA-lyase, that is, to perform a C-N bond cleavage turning choline into trimethylamine (TMA) plus acetaldehyde. The gene symbol is cutC, for choline utilization. The activase, CutD, is a radical SAM enzyme. [Energy metabolism, Amino acids and amines]
Pssm-ID: 275187 [Multi-domain]
Cd Length: 789
Bit Score: 461.96
E-value: 3.98e-151
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 9 LS ER IKAH K TALVH i VK P PVCTE RA QHY TE MYQQHMDK P IPVR RA L A LAHHLAKRTIW I KH DELI I G NQASEV RA APIF P 88
Cdd:TIGR04394 1 PT ER LVRL K ENYLK - QV P SITIY RA RAI TE IAKENPGM P KILL RA K A FRKCCETAPLV I QD DELI V G APCGAP RA GAFS P 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 89 EYTVS W IEK E I D DLAD RP GAG F AV SEE N K RVL - H EI C P W W R G QTVQDR C YGMF td EQK G LL E tgi IKA E GNMTSGDA H l A 167
Cdd:TIGR04394 80 DIAWR W LRD E L D TIGT RP QDP F YI SEE D K KIM r E EI F P F W E G KSLDEY C EDQY -- REA G VW E --- LSG E SFVSDCSY H - A 153
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 168 VN -------- FPLV L - E KG LDGLR A KVA E RRSRINLTVL ED LHGDQ F L K AIDIVL E A V SLHI KR FADL A R E M A ST E TRVS 238
Cdd:TIGR04394 154 VN gggdsnpg YDVI L m K KG MLDIQ A EAK E HLAELSYENP ED IDKIY F Y K SVIDTT E G V MIYA KR LSEY A A E L A AK E QDPK 233
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 239 R RD EL LAM AE - N CD V I AH e P P K TFW Q A L Q LCYFIQLI L QI E S N GHSV S FA R M DQY L YP Y Y RR D V E l NQSLDREH A I EL LH 317
Cdd:TIGR04394 234 R KA EL EKI AE v N AR V P AH - K P R TFW E A I Q SVWTVESL L VV E E N QTGM S IG R V DQY M YP F Y KA D I E - AGRMTEYE A F EL AG 311
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 318 SCWL K LL E VNKIR S GSH SK AS AG SPLYQ N VTI GG QKL vng E PM DA V N P L S Y AILESCGRLRST QP N L SV R Y H AGMSNDF L 397
Cdd:TIGR04394 312 CMLI K MS E MMWLT S EGG SK FF AG YQPFV N MCV GG VTR --- E GG DA T N D L T Y LLMDAVRHVKVY QP S L AC R I H NKSPQKY L 388
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 398 DAC V Q V I R C G F G M PA FNN D EIV I PEFIKL GV ERD DA Y DY AAI GC I E TAVG G KW g Y RC T GMSFINFARVMLAA L EG G RDAT 477
Cdd:TIGR04394 389 KKI V D V V R A G M G F PA CHF D DAH I KMMLAK GV SIE DA R DY CLM GC V E PQKS G RL - Y QW T STAYTQWPICIELV L NH G VPLW 467
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 478 S GK VFL P QEKA LS ag N FD NF E EVM AA WDT QI R Y Y T RK S ieiey V V D T MLEEN VH - D I ---- L C S ALVDD C I E RA K SIKQ G 552
Cdd:TIGR04394 468 Y GK QVC P DTGD LS -- Q FD TY E KFD AA VKE QI K Y I T KW S ----- A V A T VISQR VH r D L apkp L M S LMYEG C M E KG K DVSA G 540
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 553 GA K Y DWVS G L - QV G I A NLGN S L AA V KKLV FEQGVIGQQ QL AA AL ADD F D G lt H EQ L R QRLIN g APKYGNDDD SV D MLLT - 630
Cdd:TIGR04394 541 GA M Y NFGP G V v WS G L A TYAD S M AA I KKLV YDDKKYTLE QL NE AL KAN F E G -- Y EQ I R ADCLD - APKYGNDDD YA D LIAA d 617
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 631 --------- R A Y Q T YIEE L KQ yhnprygrgpiggnyya GT S SIS A N V PFG AA T M A TPD GR K A H TPL AE G A SP AS G T D HL G 701
Cdd:TIGR04394 618 lvnftereh R K Y K T LYSH L SH ----------------- GT L SIS N N T PFG QL T G A SAN GR L A W TPL SD G I SP TQ G A D FK G 680
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 702 PTA V I G SV G K LPTEAILG G VLL N Q KL NPST L ENDSDRQK L MV LLRT FFEVHK G w HI Q Y N IVSR ETLL E A KK HP DQ YRDLV 781
Cdd:TIGR04394 681 PTA I I K SV S K MANDSMNI G MVH N F KL MSGL L DTPEGENG L IT LLRT ASILGN G - EM Q F N YLDN ETLL D A QQ HP EK YRDLV 759
810 820
....*....|....*....|....*
gi 1730594474 782 VRVAGYSAFF TA L SP D A QD D II A RT 806
Cdd:TIGR04394 760 VRVAGYSAFF VE L CK D V QD E II S RT 784
PFL1
cd01678 Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, ...
68-808
4.63e-56
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate in which two cysteines and one glycine form radicals that are required for catalysis. PFL has a ten-stranded alpha/beta barrel domain that is structurally similar to those of all three ribonucleotide reductase (RNR) classes as well as benzylsuccinate synthase and B12-independent glycerol dehydratase.
Pssm-ID: 153087 [Multi-domain]
Cd Length: 738
Bit Score: 205.68
E-value: 4.63e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 68 K HD E L I I G N Q ASEVRAAP I F P EYTVSWI E KEIDD ladrpg A G FAVSE E nkrv L HE I CPWW R g Q T VQ D RCYGMF T D E QKGL 147
Cdd:cd01678 82 K EL E V I V G L Q TDKPLKRA I M P FGGIRMA E QALKA ------ Y G YELDP E ---- L KK I FTKY R - K T HN D GVFDAY T P E IRRA 150
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 148 LET GII kaegnmt S G -- DA H ---- LAVNFPL V LEK G L D G L rak VA E RRSR inltv L ED L H GD QFLKAIDIVL E A V SLH IK 221
Cdd:cd01678 151 RHS GII ------- T G lp DA Y grgr IIGDYRR V ALY G V D R L --- IE E KKKD ----- L DN L G GD EMTDDTIRLR E E V AEQ IK 215
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 222 RFAD L AREM AS TETRV SR rdellamaencdviahe P PKTFWQ A L Q LC YF IQ L ILQI E S NG HSV S FA R MDQY L YP Y YR RD V 301
Cdd:cd01678 216 ALKE L KQMA AS YGLDI SR ----------------- P ATNAQE A I Q WT YF GY L AAIK E Q NG AAM S LG R VSTF L DI Y IE RD L 278
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 302 E l NQSLDREH A I EL LHSCWL KL LE V NKI R SGSHSKASA G S P LYQNVT IGG Q k LVN G EPM da V NPL S YAI L ESCGR L - RST 380
Cdd:cd01678 279 K - AGTITEAE A Q EL IDQFIM KL RM V RFL R TPEYNELFS G D P TWVTES IGG M - GND G RSL -- V TKT S FRF L NTLYN L g PAP 354
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 381 Q PNL S V RYHAGMSND F LDA C VQ V ------ I R cgfgmpa FN ND EIVI P EF iklgverdd AY D YAA I G C IET A VG - GK ---- 449
Cdd:cd01678 355 E PNL T V LWSEKLPEN F KRF C AK V sidtss I Q ------- YE ND DLMR P DW --------- GG D DYG I A C CVS A MR i GK qmqf 418
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 450 W G Y R C tgmsfi N F A RVM L A A LE GGRD ATS G KVFL P QEKALSAGNF D n FE EVM AAW D TQIRYYTRKSIEIEYVVDT M LEEN 529
Cdd:cd01678 419 F G A R A ------ N L A KAL L Y A IN GGRD EKT G DQVG P DIEPITSDYL D - YD EVM ENY D KSMDWLADTYVNALNIIHY M HDKY 491
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 530 VHDI L CS AL V D DCIE R A ksikqggakydwvsg LQV GIA N L GN --- SL A A V K ---- K LVFE qgvigqqql AAA LA D DF D gl 602
Cdd:cd01678 492 AYEA L QM AL H D TDVR R T --------------- MAF GIA G L SV aad SL S A I K yakv K PIRD --------- EDG LA V DF E -- 545
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 603 theqlrqrl I N G A - P K YGNDDD SV D MLLTRAYQ T YIEE L KQYHNP R ygrgpigg N YYAGT S -- S I SA NV PF G AA T MA TPD 679
Cdd:cd01678 546 --------- I E G D f P R YGNDDD RA D DIAVWVVK T FMNK L RKHKTY R -------- N AEPTQ S vl T I TS NV VY G KK T GN TPD 608
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 680 GR K A HT P L A E GA S P AS G T D HL G PT A VIG SV G KLP TEAILG G VLLNQKLN P ST L ENDSDRQK -- L MVL L RTF F e VHK G W H I 757
Cdd:cd01678 609 GR R A GE P F A P GA N P MH G R D KK G AL A SLA SV A KLP YRDAND G ISNTFSIV P NA L GKTDEERI dn L VGI L DGY F - TKG G H H L 687
730 740 750 760 770
....*....|....*....|....*....|....*....|....*....|.
gi 1730594474 758 QY N IVS RETLL E A KK HP DQ Y RD L VV RV A GY SAF F TA L SPDA Q D D I I A RT E H 808
Cdd:cd01678 688 NV N VLN RETLL D A ME HP EK Y PQ L TI RV S GY AVN F VK L TREQ Q L D V I S RT F H 738
Gly_radical
pfam01228 Glycine radical;
687-792
2.22e-40
Glycine radical;
Pssm-ID: 426140 [Multi-domain]
Cd Length: 106
Bit Score: 143.85
E-value: 2.22e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 687 L A E G A SP AS G T D HL GPTAV IG SVGK LPT E AI L G G VL LNQK LN P ST L ENDSD -- RQK L MV L LR T F F ev HK G W H I Q Y N I V S R 764
Cdd:pfam01228 1 V A P G I SP SH G A D FE GPTAV LN SVGK IDY E VE L D G IS LNQK FL P AV L GYYDE eg YAN L NT L ID T Y F -- EG G H H L Q F N V V D R 78
90 100
....*....|....*....|....*...
gi 1730594474 765 ETL LE A K KHP DQ Y R DL V VRV A GYSA F F T 792
Cdd:pfam01228 79 ETL PD A Q KHP EK Y P DL T VRV S GYSA N F V 106
PRK11127
PRK11127 autonomous glycyl radical cofactor GrcA; Provisional
751-808
2.88e-15
autonomous glycyl radical cofactor GrcA; Provisional
Pssm-ID: 182983 [Multi-domain]
Cd Length: 127
Bit Score: 72.95
E-value: 2.88e-15
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*...
gi 1730594474 751 V HK G W H IQY N IVS RETL LE A K KHP DQ Y RD L VV RV A GY SAF F TA L S P DA Q D D I IART EH 808
Cdd:PRK11127 67 V EG G Q H LNV N VLR RETL ED A V KHP EK Y PQ L TI RV S GY AVR F NS L T P EQ Q R D V IART FT 124
RNR_PFL
cd00576 Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and ...
242-467
6.11e-15
Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and pyruvate formate lyase (PFL) are believed to have diverged from a common ancestor. They have a structurally similar ten-stranded alpha-beta barrel domain that hosts the active site, and are radical enzymes. RNRs are found in all organisms and provide the only mechanism by which nucleotides are converted to deoxynucleotides. RNRs are separated into three classes based on their metallocofactor usage. Class I RNRs use a diiron-tyrosyl radical while Class II RNRs use coenzyme B12 (adenosylcobalamin, AdoCbl). Class III RNRs use an FeS cluster and S-adenosylmethionine to generate a glycyl radical. PFL, an essential enzyme in anaerobic bacteria, catalyzes the conversion of pyruvate and CoA to acteylCoA and formate in a mechanism that uses a glycyl radical.
Pssm-ID: 153083 [Multi-domain]
Cd Length: 401
Bit Score: 77.57
E-value: 6.11e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 242 ELLAMAENCDVIAH E PP K TFWQ A L Q LC Y F I QLILQIES NG HS VSFAR MDQY L Y PY YR RD VEL n Q S LDREH A I E LLHSCW L 321
Cdd:cd00576 29 DYGDSLDPGIKGVN E TA K SINE A I Q KT Y Q I IALAASNQ NG GG VSFAR ASSI L S PY GS RD YAK - G S GTETD A V E AADAFN L 107
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 322 K L L EV NK irsgshska SA G SPLYQNVT IGG QKL v NGEPMDAVNP L SY A ILESCGRLRSTQ PNLSVR YHAGMSN ------ D 395
Cdd:cd00576 108 A L K EV GQ --------- GN G RTGAATGF IGG VHK - GKGDKISQEF L NL A LANGGEGIPLNF PNLSVR VSSDKPG ilvkav E 177
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730594474 396 FLDACVQVI R c GF G M P AFN NDE I -------------- V I P ----------- E FIK L G V ERD D AYDYAAIGC I E T AVG G KW 450
Cdd:cd00576 178 LKQLIAEEA R - KT G S P GIF NDE L cnlvslnlarimek A I N gsmdvvleele E LAF L A V RAL D CVIDSHDER I P T IEL G GD 256
250
....*....|....*..
gi 1730594474 451 GY R CT G MSFINF A RVML 467
Cdd:cd00576 257 ER R TV G LGIAGV A DLLI 273
Blast search parameters
Data Source:
Precalculated data, version = cdd.v.3.21
Preset Options: Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
