NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1730689535|gb|TYG44445|]
View 

hypothetical protein ES288_D11G097200v1 [Gossypium darwinii]

Protein Classification

glycoside hydrolase family 16 protein( domain architecture ID 10465002)

glycoside hydrolase family 16 protein is one of a widespread group of enzymes that hydrolyze the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety

PubMed:  31501245
SCOP:  4002662

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
Glyco_hydro_16 pfam00722
Glycosyl hydrolases family 16;
31-193 5.91e-49

Glycosyl hydrolases family 16;


:

Pssm-ID: 395585 [Multi-domain]  Cd Length: 168  Bit Score: 157.37  E-value: 5.91e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  31 SNSITLTFDHRGGARWRSTTRFLYGTFTSLIQcpKGNTSGLNFNIYLSSlEGDKSQDEIDFEFLGKDKTIVQTNYYTTGT 110
Cdd:pfam00722  10 NGGLTLTLDKYTGSGFQSKFYYLYGKVEARIK--AARGAGVVTAFYLSS-EDWDDHDEIDFEFLGNDTGQVQTNVYGNGK 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535 111 GNR-EQIHDLGFDCSDGFHEYTIKWNPDSIEWVIDGKVVR--KAEKKEGEAFPEKPMFLYASVWDASYiaegqWTGPYIG 187
Cdd:pfam00722  87 GNRgEQRFSLWFDPTADFHTYSILWNPDKITWYVDGVPVRtlKNNDAGGVPYPQTPMRLYVSLWPGGD-----WATPGGG 161


                  ....*.
gi 1730689535 188 CDVPYV 193
Cdd:pfam00722 162 VKIDWA 167
 
Name Accession Description Interval E-value
Glyco_hydro_16 pfam00722
Glycosyl hydrolases family 16;
31-193 5.91e-49

Glycosyl hydrolases family 16;


Pssm-ID: 395585 [Multi-domain]  Cd Length: 168  Bit Score: 157.37  E-value: 5.91e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  31 SNSITLTFDHRGGARWRSTTRFLYGTFTSLIQcpKGNTSGLNFNIYLSSlEGDKSQDEIDFEFLGKDKTIVQTNYYTTGT 110
Cdd:pfam00722  10 NGGLTLTLDKYTGSGFQSKFYYLYGKVEARIK--AARGAGVVTAFYLSS-EDWDDHDEIDFEFLGNDTGQVQTNVYGNGK 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535 111 GNR-EQIHDLGFDCSDGFHEYTIKWNPDSIEWVIDGKVVR--KAEKKEGEAFPEKPMFLYASVWDASYiaegqWTGPYIG 187
Cdd:pfam00722  87 GNRgEQRFSLWFDPTADFHTYSILWNPDKITWYVDGVPVRtlKNNDAGGVPYPQTPMRLYVSLWPGGD-----WATPGGG 161


                  ....*.
gi 1730689535 188 CDVPYV 193
Cdd:pfam00722 162 VKIDWA 167
GH16_XET cd02176
Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16; Xyloglucan ...
 32-177 5.41e-44

Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16; Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling.


Pssm-ID: 185685 [Multi-domain]  Cd Length: 263  Bit Score: 147.73  E-value: 5.41e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  32 NSITLTFDHRGGARWRSTTRFLYGTFTSLIQCPKGNTSGLNFNIYLSSlEGDKSQDEIDFEFLGK---DKTIVQTNYYTT 108
Cdd:cd02176    26 TSVQLTLDQSSGSGFKSKNKYLFGFFSMRIKLPPGDSAGTVTAFYLSS-QGPDNHDEIDFEFLGNvtgQPYTLQTNVFAN 104

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1730689535 109 GTGNREQIHDLGFDCSDGFHEYTIKWNPDSIEWVIDGKVVR--KAEKKEGEAFP-EKPMFLYASVWDASYIA 177
Cdd:cd02176   105 GVGGREQRIYLWFDPTADFHTYSILWNPHQIVFYVDDVPIRvfKNNEALGVPYPsSQPMGVYASIWDGSDWA 176
PLN03161 PLN03161
Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional
 32-173 6.68e-30

Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional


Pssm-ID: 178706 [Multi-domain]  Cd Length: 291  Bit Score: 111.92  E-value: 6.68e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  32 NSITLTFDHRGGARWRSTTRFLYGTFTSLIQCPKGNTSGLNFNIYLSSlEGDKsQDEIDFEFLGK---DKTIVQTNYYTT 108
Cdd:PLN03161   47 DNLQLVLDQSSGSGIKSKRAFLFGSIEMLIKLVPGNSAGTVTAYYLSS-TGSR-HDEIDFEFLGNvsgQPYTIHTNIYTQ 124

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1730689535 109 GTGNREQIHDLGFDCSDGFHEYTIKWNPDSIEWVIDG---KVVRKAEkKEGEAFPEKP-MFLYASVWDA 173
Cdd:PLN03161  125 GNGSREQQFRPWFDPTADFHNYTIHWNPSEVVWYVDGtpiRVFRNYE-NEGIAYPNKQgMRVYSSLWNA 192
BglS COG2273
Beta-glucanase, GH16 family [Carbohydrate transport and metabolism];
20-185 6.96e-22

Beta-glucanase, GH16 family [Carbohydrate transport and metabolism];


Pssm-ID: 441874 [Multi-domain]  Cd Length: 259  Bit Score: 90.05  E-value: 6.96e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  20 YTPEACThcpVSNSI-TLTF----DHRGGARWRS---TTR----FLYGTFTSLIQCPKGntSGLNFNIYL---SSLEGDK 84
Cdd:COG2273    64 YTDENVS---VENGNlVITArkepYGGGGRPYTSgriTTKgkfsFTYGRFEARAKLPKG--QGLWPAFWMlggDIDGGWP 138

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  85 SQDEIDF-EFLGKDKTIVQTNYYTTGTGNREQI---HDLGFDCSDGFHEYTIKWNPDSIEWVIDGKVVRKAEKKEGEAFP 160
Cdd:COG2273   139 ASGEIDImEFVGKDPNKVHGNVHYGGYNGGEGIgasYDLPFDASDDFHTYAVEWTPDSIRWYVDGVLVHTVTPADVGGPW 218

                         170       180
                  ....*....|....*....|....*..
gi 1730689535 161 E--KPMFLYASVWdasyiAEGQWTGPY 185
Cdd:COG2273   219 PfdQPFYLILNLA-----VGGNWPGAP 240
 
Name Accession Description Interval E-value
Glyco_hydro_16 pfam00722
Glycosyl hydrolases family 16;
31-193 5.91e-49

Glycosyl hydrolases family 16;


Pssm-ID: 395585 [Multi-domain]  Cd Length: 168  Bit Score: 157.37  E-value: 5.91e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  31 SNSITLTFDHRGGARWRSTTRFLYGTFTSLIQcpKGNTSGLNFNIYLSSlEGDKSQDEIDFEFLGKDKTIVQTNYYTTGT 110
Cdd:pfam00722  10 NGGLTLTLDKYTGSGFQSKFYYLYGKVEARIK--AARGAGVVTAFYLSS-EDWDDHDEIDFEFLGNDTGQVQTNVYGNGK 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535 111 GNR-EQIHDLGFDCSDGFHEYTIKWNPDSIEWVIDGKVVR--KAEKKEGEAFPEKPMFLYASVWDASYiaegqWTGPYIG 187
Cdd:pfam00722  87 GNRgEQRFSLWFDPTADFHTYSILWNPDKITWYVDGVPVRtlKNNDAGGVPYPQTPMRLYVSLWPGGD-----WATPGGG 161


                  ....*.
gi 1730689535 188 CDVPYV 193
Cdd:pfam00722 162 VKIDWA 167
GH16_XET cd02176
Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16; Xyloglucan ...
 32-177 5.41e-44

Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16; Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling.


Pssm-ID: 185685 [Multi-domain]  Cd Length: 263  Bit Score: 147.73  E-value: 5.41e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  32 NSITLTFDHRGGARWRSTTRFLYGTFTSLIQCPKGNTSGLNFNIYLSSlEGDKSQDEIDFEFLGK---DKTIVQTNYYTT 108
Cdd:cd02176    26 TSVQLTLDQSSGSGFKSKNKYLFGFFSMRIKLPPGDSAGTVTAFYLSS-QGPDNHDEIDFEFLGNvtgQPYTLQTNVFAN 104

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1730689535 109 GTGNREQIHDLGFDCSDGFHEYTIKWNPDSIEWVIDGKVVR--KAEKKEGEAFP-EKPMFLYASVWDASYIA 177
Cdd:cd02176   105 GVGGREQRIYLWFDPTADFHTYSILWNPHQIVFYVDDVPIRvfKNNEALGVPYPsSQPMGVYASIWDGSDWA 176
GH16_lichenase cd02175
lichenase, member of glycosyl hydrolase family 16; Lichenase, also known as 1,3-1, ...
 43-185 2.56e-37

lichenase, member of glycosyl hydrolase family 16; Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.


Pssm-ID: 185684 [Multi-domain]  Cd Length: 212  Bit Score: 128.93  E-value: 2.56e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  43 GARWRSTTRFLYGTFTSLIQCPKGntSGLN--FNIYLSSLEGDKsQDEIDFEFLGKDKTIVQTNYYTTGTGNREQIHDLG 120
Cdd:cd02175    57 CGEYRTRGFYGYGRYEVRMKPAKG--SGVVssFFTYTGPYDGDP-HDEIDIEFLGKDTTKVQFNYYTNGVGGHEKLIDLG 133

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1730689535 121 FDCSDGFHEYTIKWNPDSIEWVIDGKVVRKAEKKEGEaFPEKPMFLYASVWDASYIAEgqWTGPY 185
Cdd:cd02175   134 FDASEGFHTYAFEWEPDSIRWYVDGELVHEATATDPN-IPDTPGKIMMNLWPGDGVDD--WLGPF 195
GH16_fungal_CRH1_transglycosylase cd02183
glycosylphosphatidylinositol-glucanosyltransferase; Group of fungal GH16 members related to ...
 31-201 1.79e-30

glycosylphosphatidylinositol-glucanosyltransferase; Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.


Pssm-ID: 185692 [Multi-domain]  Cd Length: 203  Bit Score: 111.10  E-value: 1.79e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  31 SNSITLTFDHRG-GARWRSTTRFLYGTFTSLIQCPKGN---TSglnfnIYLSSlegdKSQDEIDFEFLGKDKTIVQTNYY 106
Cdd:cd02183    23 DDGASLTIPKRGdGPTISSTFYIFYGKVEVTMKAAPGQgivSS-----FVLQS----DDLDEIDWEWVGGDLTQVQTNYF 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535 107 ---TTGTGNREQIHDLGFDCSDGFHEYTIKWNPDSIEWVIDGKVVR---KAEKKEGEAFPEKPMFLYASVW---DASYiA 177
Cdd:cd02183    94 gkgNTTTYDRGGYHPVPNPQTEEFHTYTIDWTKDRITWYIDGKVVRtltKADTTGGYGYPQTPMRLQIGIWaggDPSN-A 172

                         170       180
                  ....*....|....*....|....*..
gi 1730689535 178 EG--QWTGPYIGCD-VPYVCLYKDIQV 201
Cdd:cd02183   173 PGtiEWAGGETDYDkGPFTMYVKSVTV 199
PLN03161 PLN03161
Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional
 32-173 6.68e-30

Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional


Pssm-ID: 178706 [Multi-domain]  Cd Length: 291  Bit Score: 111.92  E-value: 6.68e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  32 NSITLTFDHRGGARWRSTTRFLYGTFTSLIQCPKGNTSGLNFNIYLSSlEGDKsQDEIDFEFLGK---DKTIVQTNYYTT 108
Cdd:PLN03161   47 DNLQLVLDQSSGSGIKSKRAFLFGSIEMLIKLVPGNSAGTVTAYYLSS-TGSR-HDEIDFEFLGNvsgQPYTIHTNIYTQ 124

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1730689535 109 GTGNREQIHDLGFDCSDGFHEYTIKWNPDSIEWVIDG---KVVRKAEkKEGEAFPEKP-MFLYASVWDA 173
Cdd:PLN03161  125 GNGSREQQFRPWFDPTADFHNYTIHWNPSEVVWYVDGtpiRVFRNYE-NEGIAYPNKQgMRVYSSLWNA 192
BglS COG2273
Beta-glucanase, GH16 family [Carbohydrate transport and metabolism];
20-185 6.96e-22

Beta-glucanase, GH16 family [Carbohydrate transport and metabolism];


Pssm-ID: 441874 [Multi-domain]  Cd Length: 259  Bit Score: 90.05  E-value: 6.96e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  20 YTPEACThcpVSNSI-TLTF----DHRGGARWRS---TTR----FLYGTFTSLIQCPKGntSGLNFNIYL---SSLEGDK 84
Cdd:COG2273    64 YTDENVS---VENGNlVITArkepYGGGGRPYTSgriTTKgkfsFTYGRFEARAKLPKG--QGLWPAFWMlggDIDGGWP 138

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  85 SQDEIDF-EFLGKDKTIVQTNYYTTGTGNREQI---HDLGFDCSDGFHEYTIKWNPDSIEWVIDGKVVRKAEKKEGEAFP 160
Cdd:COG2273   139 ASGEIDImEFVGKDPNKVHGNVHYGGYNGGEGIgasYDLPFDASDDFHTYAVEWTPDSIRWYVDGVLVHTVTPADVGGPW 218

                         170       180
                  ....*....|....*....|....*..
gi 1730689535 161 E--KPMFLYASVWdasyiAEGQWTGPY 185
Cdd:COG2273   219 PfdQPFYLILNLA-----VGGNWPGAP 240
Glyco_hydrolase_16 cd00413
glycosyl hydrolase family 16; The O-Glycosyl hydrolases are a widespread group of enzymes that ...
 50-183 1.25e-19

glycosyl hydrolase family 16; The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.


Pssm-ID: 185683 [Multi-domain]  Cd Length: 210  Bit Score: 82.87  E-value: 1.25e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  50 TRFLYGTFTSLIQCPkgNTSGLNFNIYLSSLEGDKSQ-DEIDFEFLGKDKTIVQTNYYT-----TGTGNREQIHDLGFDC 123
Cdd:cd00413    63 NNYTYGYYEARAKLA--GGPGAVSAFWTYSDDDDPPDgGEIDIEFLGRDPTTVQTNVHWpgygaGATTGEEKSVHLPFDP 140

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535 124 SDGFHEYTIKWNPDSIEWVIDGKVVRKAEkkegEAFPEKPMFLYASVWDasyiAEGQWTG 183
Cdd:cd00413   141 ADDFHTYRVDWTPGEITFYVDGVLVATIT----NQVPDDPMNIILNLWS----DGGWWWG 192
GH16_laminarinase_like cd08023
Laminarinase, member of the glycosyl hydrolase family 16; Laminarinase, also known as glucan ...
 88-166 2.89e-10

Laminarinase, member of the glycosyl hydrolase family 16; Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.


Pssm-ID: 185693 [Multi-domain]  Cd Length: 235  Bit Score: 58.02  E-value: 2.89e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  88 EID-FEFLGKDKTIVQTNYYTTGTGNRE-----QIHDLGFDCSDGFHEYTIKWNPDSIEWVIDGKVV----RKAEKKEGE 157
Cdd:cd08023   115 EIDiMEYVGNEPNTVYGTLHGGATNDGNngsggSYTLPTDDLSDDFHTYAVEWTPDKITFYVDGKLYftytNPNTDNGGQ 194


                  ....*....
gi 1730689535 158 AFPEKPMFL 166
Cdd:cd08023   195 WPFDQPFYL 203
GH16_beta_GRP cd02179
beta-1,3-glucan recognition protein, member of glycosyl hydrolase family 16; Beta-GRP (beta-1, ...
 124-171 1.85e-05

beta-1,3-glucan recognition protein, member of glycosyl hydrolase family 16; Beta-GRP (beta-1,3-glucan recognition protein) is one of several pattern recognition receptors (PRRs), also referred to as biosensor proteins, that complexes with pathogen-associated beta-1,3-glucans and then transduces signals necessary for activation of an appropriate innate immune response. They are present in insects and lack all catalytic residues. This subgroup also contains related proteins of unknown function that still contain the active site. Their structures adopt a jelly roll fold with a deep active site channel harboring the catalytic residues, like those of other glycosyl hydrolase family 16 members.


Pssm-ID: 185688 [Multi-domain]  Cd Length: 321  Bit Score: 44.29  E-value: 1.85e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 1730689535 124 SDGFHEYTIKWNPDSIEWVIDGKVVRKAEKKEGEaFPEKPMFLYASVW 171
Cdd:cd02179   199 SDDFHVYTLEWKPDGITLMVDGEEYGEIEPGEGG-YSEAANNPAASRW 245
GH16_beta_agarase cd02178
Beta-agarase, member of glycosyl hydrolase family 16; Beta-agarase is a glycosyl hydrolase ...
 37-166 4.34e-04

Beta-agarase, member of glycosyl hydrolase family 16; Beta-agarase is a glycosyl hydrolase family 16 (GH16) member that hydrolyzes the internal beta-1,4-linkage of agarose, a hydrophilic polysaccharide found in the cell wall of Rhodophyceaea, marine red algae. Agarose is a linear chain of galactose units linked by alternating L-alpha-1,3- and D-beta-1,4-linkages that are additionally modified by a 3,6-anhydro-bridge. Agarose forms thermo-reversible gels that are widely used in the food industry or as a laboratory medium. While beta-agarases are also found in two other families derived from the sequence-based classification of glycosyl hydrolases (GH50, and GH86) the GH16 members are most abundant. This domain adopts a curved beta-sandwich conformation, with a tunnel-shaped active site cavity, referred to as a jellyroll fold.


Pssm-ID: 185687  Cd Length: 258  Bit Score: 40.03  E-value: 4.34e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1730689535  37 TFDHRGGARwRSTTRFLYGTFTSLIqcpKGNTSGLNFNIYLSSLEGDKSQdEID-FEFLGKDKTIVQTNYYTTGT----G 111
Cdd:cd02178    82 GYKVTTGSI-TSKEKVKYGYFEARA---KASNLPMSSAFWLLSDTKDSTT-EIDiLEHYGGDREEWFATRMNSNThvfiR 156

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1730689535 112 NREQIHDLGFDCS---------DGFHEYTIKWN-PDSIEWVIDGKVVRKAEKKE--GEAFPEKPMFL 166
Cdd:cd02178   157 DPEQDYQPKDDGSwyynptelaDDFHVYGVYWKdPDTIRFYIDGVLVRTVENSEitDGTGFDQPMYI 223
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH