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Conserved domains on  [gi|446044519|ref|WP_000122374|]
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MULTISPECIES: MobV family relaxase [Bacteria]

Protein Classification

plasmid recombination protein( domain architecture ID 10471159)

plasmid recombination protein belongs to the plasmid mobilization pre family

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Mob_Pre pfam01076
Plasmid recombination enzyme; With some plasmids, recombination can occur in a site specific ...
 1-198 3.95e-79

Plasmid recombination enzyme; With some plasmids, recombination can occur in a site specific manner that is independent of RecA. In such cases, the recombination event requires another protein called Pre. Pre is a plasmid recombination enzyme. This protein is also known as Mob (conjugative mobilization). This family is also known as Mob-V. One of the family members, MobM, is encoded by a promiscuous plasmid actively involved in the spread of antibiotic resistance. Homologs of MobM are found in many plasmids and other mobile genetic elements of pathogenic bacteria, including S. aureus. MobM is a metal-dependent nuclease that uses histidine nitrogen for the nucleophilic attack on the scissile phosphate. Furthermore, in contrast to other DNA-processing enzymes, MobM is a histidine relaxase, a DNA-breaking and -joining enzyme, that operates through a phosphorus-nitrogen protein-DNA adduct for cell-to-cell DNA transfer. Mutational analysis indicate that the H(N/D)(Q/E)R N-terminal motif of MobM plays a crucial role in the cleavage and generation of stable DNA-protein adducts.


:

Pssm-ID: 395854  Cd Length: 195  Bit Score: 242.61  E-value: 3.95e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519    1 MSYSIVRVSKVKSGtNTTGIQKHVQRENNNYENEDIDHSKTYLNYDLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKH 80
Cdd:pfam01076   1 NNYAIARMQKVKAS-NLDGIQKHNQRENETYDNKDIDPERSGLNYDLVNDKQIRYDEDIKDYIKENQKSKRKIRKDAVLE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519   81 IDGLITSDNDFFDNQTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALT 160
Cdd:pfam01076  80 NEILITSDPDFFEQLTYEEAKEFFETAFQFFEERYGKENVLYAVVHLDEATPHMHMGVVPGTEDGRLSAKAIFGNRKELI 159

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 446044519  161 AFQDRFNEHVKQRGYDLERGQSRqvTNAKHEQISQYKQ 198
Cdd:pfam01076 160 ALQDRFPEYMGLKGFDLERGEPG--SERKHLGTNEYKA 195
 
Name Accession Description Interval E-value
Mob_Pre pfam01076
Plasmid recombination enzyme; With some plasmids, recombination can occur in a site specific ...
 1-198 3.95e-79

Plasmid recombination enzyme; With some plasmids, recombination can occur in a site specific manner that is independent of RecA. In such cases, the recombination event requires another protein called Pre. Pre is a plasmid recombination enzyme. This protein is also known as Mob (conjugative mobilization). This family is also known as Mob-V. One of the family members, MobM, is encoded by a promiscuous plasmid actively involved in the spread of antibiotic resistance. Homologs of MobM are found in many plasmids and other mobile genetic elements of pathogenic bacteria, including S. aureus. MobM is a metal-dependent nuclease that uses histidine nitrogen for the nucleophilic attack on the scissile phosphate. Furthermore, in contrast to other DNA-processing enzymes, MobM is a histidine relaxase, a DNA-breaking and -joining enzyme, that operates through a phosphorus-nitrogen protein-DNA adduct for cell-to-cell DNA transfer. Mutational analysis indicate that the H(N/D)(Q/E)R N-terminal motif of MobM plays a crucial role in the cleavage and generation of stable DNA-protein adducts.


Pssm-ID: 395854  Cd Length: 195  Bit Score: 242.61  E-value: 3.95e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519    1 MSYSIVRVSKVKSGtNTTGIQKHVQRENNNYENEDIDHSKTYLNYDLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKH 80
Cdd:pfam01076   1 NNYAIARMQKVKAS-NLDGIQKHNQRENETYDNKDIDPERSGLNYDLVNDKQIRYDEDIKDYIKENQKSKRKIRKDAVLE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519   81 IDGLITSDNDFFDNQTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALT 160
Cdd:pfam01076  80 NEILITSDPDFFEQLTYEEAKEFFETAFQFFEERYGKENVLYAVVHLDEATPHMHMGVVPGTEDGRLSAKAIFGNRKELI 159

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 446044519  161 AFQDRFNEHVKQRGYDLERGQSRqvTNAKHEQISQYKQ 198
Cdd:pfam01076 160 ALQDRFPEYMGLKGFDLERGEPG--SERKHLGTNEYKA 195
MobV NF041497
MobV family relaxase;
1-190 1.17e-72

MobV family relaxase;


Pssm-ID: 469385  Cd Length: 187  Bit Score: 225.52  E-value: 1.17e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519   1 MSYSIVRVSKVKSGtNTTGIQKHVQRENNNYENEDIDHSKTYLNYDLVN--ANKQNFNNLIDEKIEQNYTGKRKIRTDAI 78
Cdd:NF041497   1 MSYAVLRVEKLKGG-NLAGSQEHNERETENRTNPNADPSRTHLNFELVNgpDGPIDYLEAIKERIAENAGIKRKIRKDAV 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519  79 KHIDGLITSDNDFFDN-QTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGnKK 157
Cdd:NF041497  80 RAVEFILTGSPEFFKElADPGELKEWFEDNYDFLADRYGEENIVSAVVHLDETTPHIHATVVPITEDGRLSAKDVFG-RK 158

                        170       180       190
                 ....*....|....*....|....*....|...
gi 446044519 158 ALTAFQDRFNEHVKQRGydLERGQSRqvTNAKH 190
Cdd:NF041497 159 KLSQLQDDYAEAMKKYG--LERGISG--SEAKH 187
MobM_relaxase cd17242
relaxase domain of MobM and similar proteins; With some plasmids, recombination can occur in a ...
 5-204 4.22e-65

relaxase domain of MobM and similar proteins; With some plasmids, recombination can occur in a site-specific manner that is independent of RecA. In such cases, the recombination event requires another protein called Pre (plasmid recombination enzyme), also known as Mob (conjugative mobilization). The best characterized member of this family is encoded by the streptococcal plasmid pMV158 that recognizes the plasmid origin of transfer. MobM converts supercoiled plasmid DNA into relaxed DNA by cleaving a phosphodiester bond of a specific dinucleotide and remains bound to the 5'-end of the nick site.


Pssm-ID: 410988  Cd Length: 196  Bit Score: 206.73  E-value: 4.22e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519   5 IVRVSKVKSGtNTTGIQKHVQRENNNYeNEDIDHSKTYLNYDLVNANKQNFNNLIDEKIEQNYTgKRKIRTDAIKHIDGL 84
Cdd:cd17242    1 ILRVEKLKSG-ALGGALRHNERERETE-NPNIDPSRTHLNYELVGPGKKDYAEAIKERLEELGK-KKKIRKDAVLAVEFI 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519  85 ITSDNDFFDNQTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQD 164
Cdd:cd17242   78 LTASPEFFEGLDPEEIEEFFKDALEFLKERFGEENIVSAVVHLDETTPHLHAVVVPITEDGRLSAKELFGGRKKLRELQD 157

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 446044519 165 RFNEHVKQRgYDLERGQSRQVTNAKHEQISQYKQKTEYHK 204
Cdd:cd17242  158 DYAEAVAKK-GGLERGIKGSKAKHKTKEKELKAKHEELKE 196
 
Name Accession Description Interval E-value
Mob_Pre pfam01076
Plasmid recombination enzyme; With some plasmids, recombination can occur in a site specific ...
 1-198 3.95e-79

Plasmid recombination enzyme; With some plasmids, recombination can occur in a site specific manner that is independent of RecA. In such cases, the recombination event requires another protein called Pre. Pre is a plasmid recombination enzyme. This protein is also known as Mob (conjugative mobilization). This family is also known as Mob-V. One of the family members, MobM, is encoded by a promiscuous plasmid actively involved in the spread of antibiotic resistance. Homologs of MobM are found in many plasmids and other mobile genetic elements of pathogenic bacteria, including S. aureus. MobM is a metal-dependent nuclease that uses histidine nitrogen for the nucleophilic attack on the scissile phosphate. Furthermore, in contrast to other DNA-processing enzymes, MobM is a histidine relaxase, a DNA-breaking and -joining enzyme, that operates through a phosphorus-nitrogen protein-DNA adduct for cell-to-cell DNA transfer. Mutational analysis indicate that the H(N/D)(Q/E)R N-terminal motif of MobM plays a crucial role in the cleavage and generation of stable DNA-protein adducts.


Pssm-ID: 395854  Cd Length: 195  Bit Score: 242.61  E-value: 3.95e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519    1 MSYSIVRVSKVKSGtNTTGIQKHVQRENNNYENEDIDHSKTYLNYDLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKH 80
Cdd:pfam01076   1 NNYAIARMQKVKAS-NLDGIQKHNQRENETYDNKDIDPERSGLNYDLVNDKQIRYDEDIKDYIKENQKSKRKIRKDAVLE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519   81 IDGLITSDNDFFDNQTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALT 160
Cdd:pfam01076  80 NEILITSDPDFFEQLTYEEAKEFFETAFQFFEERYGKENVLYAVVHLDEATPHMHMGVVPGTEDGRLSAKAIFGNRKELI 159

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 446044519  161 AFQDRFNEHVKQRGYDLERGQSRqvTNAKHEQISQYKQ 198
Cdd:pfam01076 160 ALQDRFPEYMGLKGFDLERGEPG--SERKHLGTNEYKA 195
MobV NF041497
MobV family relaxase;
1-190 1.17e-72

MobV family relaxase;


Pssm-ID: 469385  Cd Length: 187  Bit Score: 225.52  E-value: 1.17e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519   1 MSYSIVRVSKVKSGtNTTGIQKHVQRENNNYENEDIDHSKTYLNYDLVN--ANKQNFNNLIDEKIEQNYTGKRKIRTDAI 78
Cdd:NF041497   1 MSYAVLRVEKLKGG-NLAGSQEHNERETENRTNPNADPSRTHLNFELVNgpDGPIDYLEAIKERIAENAGIKRKIRKDAV 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519  79 KHIDGLITSDNDFFDN-QTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGnKK 157
Cdd:NF041497  80 RAVEFILTGSPEFFKElADPGELKEWFEDNYDFLADRYGEENIVSAVVHLDETTPHIHATVVPITEDGRLSAKDVFG-RK 158

                        170       180       190
                 ....*....|....*....|....*....|...
gi 446044519 158 ALTAFQDRFNEHVKQRGydLERGQSRqvTNAKH 190
Cdd:NF041497 159 KLSQLQDDYAEAMKKYG--LERGISG--SEAKH 187
MobM_relaxase cd17242
relaxase domain of MobM and similar proteins; With some plasmids, recombination can occur in a ...
 5-204 4.22e-65

relaxase domain of MobM and similar proteins; With some plasmids, recombination can occur in a site-specific manner that is independent of RecA. In such cases, the recombination event requires another protein called Pre (plasmid recombination enzyme), also known as Mob (conjugative mobilization). The best characterized member of this family is encoded by the streptococcal plasmid pMV158 that recognizes the plasmid origin of transfer. MobM converts supercoiled plasmid DNA into relaxed DNA by cleaving a phosphodiester bond of a specific dinucleotide and remains bound to the 5'-end of the nick site.


Pssm-ID: 410988  Cd Length: 196  Bit Score: 206.73  E-value: 4.22e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519   5 IVRVSKVKSGtNTTGIQKHVQRENNNYeNEDIDHSKTYLNYDLVNANKQNFNNLIDEKIEQNYTgKRKIRTDAIKHIDGL 84
Cdd:cd17242    1 ILRVEKLKSG-ALGGALRHNERERETE-NPNIDPSRTHLNYELVGPGKKDYAEAIKERLEELGK-KKKIRKDAVLAVEFI 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446044519  85 ITSDNDFFDNQTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQD 164
Cdd:cd17242   78 LTASPEFFEGLDPEEIEEFFKDALEFLKERFGEENIVSAVVHLDETTPHLHAVVVPITEDGRLSAKELFGGRKKLRELQD 157

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 446044519 165 RFNEHVKQRgYDLERGQSRQVTNAKHEQISQYKQKTEYHK 204
Cdd:cd17242  158 DYAEAVAKK-GGLERGIKGSKAKHKTKEKELKAKHEELKE 196
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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