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Conserved domains on  [gi|446194926|ref|WP_000272781|]
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MULTISPECIES: Crp/Fnr family transcriptional regulator [Staphylococcus]

Protein Classification

Crp/Fnr family transcriptional regulator( domain architecture ID 11429533)

Crp/Fnr family transcriptional regulator containing a DNA-binding Crp-like helix-turn-helix (HTH) domain, may bind cyclic nucleotides

CATH:  2.60.120.10|1.10.10.10
Gene Ontology:  GO:0003677|GO:0030552
PubMed:  11407111|14638413

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Crp COG0664
cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal ...
 38-228 1.10e-24

cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms];


:

Pssm-ID: 440428 [Multi-domain]  Cd Length: 207  Bit Score: 96.59  E-value: 1.10e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926  38 EDLTLYQYKKGQVIYHSTDQIKFVYFLVNGCILHESSNITGDNY-LRLSKDENIFPMNFIFNETPAPYEIcTALTDCKIL 116
Cdd:COG0664   14 AHLELRTLKKGEVLFREGDPADHLYFVLSGLVKLYRISEDGREQiLGFLGPGDFFGELSLLGGEPSPATA-EALEDSELL 92

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926 117 TLPKDLLEYLCRKHNEIFESLFKKLNETIQFQVEYIMALRANSAKERIERILqilcLSIGDDNGEFYELKqiMTVQLISN 196
Cdd:COG0664   93 RIPREDLEELLERNPELARALLRLLARRLRQLQERLVSLAFLSAEERLARFL----LELADRLDGRIDLP--LTQEEIAS 166

                        170       180       190
                 ....*....|....*....|....*....|..
gi 446194926 197 LSGLNRKTTGEIIRELKIENIIYQDKRNWIIK 228
Cdd:COG0664  167 YLGLTRETVSRILKKLEKEGLIELERGRITIL 198
 
Name Accession Description Interval E-value
Crp COG0664
cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal ...
 38-228 1.10e-24

cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms];


Pssm-ID: 440428 [Multi-domain]  Cd Length: 207  Bit Score: 96.59  E-value: 1.10e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926  38 EDLTLYQYKKGQVIYHSTDQIKFVYFLVNGCILHESSNITGDNY-LRLSKDENIFPMNFIFNETPAPYEIcTALTDCKIL 116
Cdd:COG0664   14 AHLELRTLKKGEVLFREGDPADHLYFVLSGLVKLYRISEDGREQiLGFLGPGDFFGELSLLGGEPSPATA-EALEDSELL 92

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926 117 TLPKDLLEYLCRKHNEIFESLFKKLNETIQFQVEYIMALRANSAKERIERILqilcLSIGDDNGEFYELKqiMTVQLISN 196
Cdd:COG0664   93 RIPREDLEELLERNPELARALLRLLARRLRQLQERLVSLAFLSAEERLARFL----LELADRLDGRIDLP--LTQEEIAS 166

                        170       180       190
                 ....*....|....*....|....*....|..
gi 446194926 197 LSGLNRKTTGEIIRELKIENIIYQDKRNWIIK 228
Cdd:COG0664  167 YLGLTRETVSRILKKLEKEGLIELERGRITIL 198
CAP_ED cd00038
effector domain of the CAP family of transcription factors; members include CAP (or cAMP ...
 45-133 8.81e-10

effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO sensor. In all cases binding of the effector leads to conformational changes and the ability to activate transcription. Cyclic nucleotide-binding domain similar to CAP are also present in cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) and vertebrate cyclic nucleotide-gated ion-channels. Cyclic nucleotide-monophosphate binding domain; proteins that bind cyclic nucleotides (cAMP or cGMP) share a structural domain of about 120 residues; the best studied is the prokaryotic catabolite gene activator, CAP, where such a domain is known to be composed of three alpha-helices and a distinctive eight-stranded, antiparallel beta-barrel structure; three conserved glycine residues are thought to be essential for maintenance of the structural integrity of the beta-barrel; CooA is a homodimeric transcription factor that belongs to CAP family; cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) contain two tandem copies of the cyclic nucleotide-binding domain; cAPK's are composed of two different subunits, a catalytic chain and a regulatory chain, which contains both copies of the domain; cGPK's are single chain enzymes that include the two copies of the domain in their N-terminal section; also found in vertebrate cyclic nucleotide-gated ion-channels


Pssm-ID: 237999 [Multi-domain]  Cd Length: 115  Bit Score: 54.64  E-value: 8.81e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926  45 YKKGQVIYHSTDQIKFVYFLVNGCI-LHESSNITGDNYLRLSKDENIFPMNFIFNETPAPYEIcTALTDCKILTLPKDLL 123
Cdd:cd00038   22 FPAGEVIIRQGDPADSLYIVLSGSVeVYKLDEDGREQIVGFLGPGDLFGELALLGNGPRSATV-RALTDSELLVLPRSDF 100

                         90
                 ....*....|
gi 446194926 124 EYLCRKHNEI 133
Cdd:cd00038  101 RRLLQEYPEL 110
cNMP_binding pfam00027
Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, ...
 44-130 1.23e-09

Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, oxygen and 2-oxoglutarate (Matilla et. al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).


Pssm-ID: 459637 [Multi-domain]  Cd Length: 89  Bit Score: 53.38  E-value: 1.23e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926   44 QYKKGQVIYHSTDQIKFVYFLVNGCIlhESSNITGD---NYLRLSKDENIFPMNFIFNETPAPYEIcTALTDCKILTLPK 120
Cdd:pfam00027   3 SYKAGEVIFREGDPADSLYIVLSGKV--KVYRTLEDgreQILAVLGPGDFFGELALLGGEPRSATV-VALTDSELLVIPR 79

                          90
                  ....*....|
gi 446194926  121 DLLEYLCRKH 130
Cdd:pfam00027  80 EDFLELLERD 89
cNMP smart00100
Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a ...
 45-124 2.31e-03

Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a prokaryotic homologue of eukaryotic cNMP-binding domains, present in ion channels, and cNMP-dependent kinases.


Pssm-ID: 197516 [Multi-domain]  Cd Length: 120  Bit Score: 36.61  E-value: 2.31e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926    45 YKKGQVIYHSTDQIKFVYFLVNGC--ILHESSNiTGDNYLRLSKDENIFPMNFIFNETPAPYEI-CTALTDCKILTLPKD 121
Cdd:smart00100  22 YPAGEVIIRQGDVGDSFYIIVSGEveVYKVLED-GEEQIVGTLGPGDFFGELALLTNSRRAASAaAVALELATLLRIDFR 100


                   ...
gi 446194926   122 LLE 124
Cdd:smart00100 101 DFL 103
 
Name Accession Description Interval E-value
Crp COG0664
cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal ...
 38-228 1.10e-24

cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms];


Pssm-ID: 440428 [Multi-domain]  Cd Length: 207  Bit Score: 96.59  E-value: 1.10e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926  38 EDLTLYQYKKGQVIYHSTDQIKFVYFLVNGCILHESSNITGDNY-LRLSKDENIFPMNFIFNETPAPYEIcTALTDCKIL 116
Cdd:COG0664   14 AHLELRTLKKGEVLFREGDPADHLYFVLSGLVKLYRISEDGREQiLGFLGPGDFFGELSLLGGEPSPATA-EALEDSELL 92

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926 117 TLPKDLLEYLCRKHNEIFESLFKKLNETIQFQVEYIMALRANSAKERIERILqilcLSIGDDNGEFYELKqiMTVQLISN 196
Cdd:COG0664   93 RIPREDLEELLERNPELARALLRLLARRLRQLQERLVSLAFLSAEERLARFL----LELADRLDGRIDLP--LTQEEIAS 166

                        170       180       190
                 ....*....|....*....|....*....|..
gi 446194926 197 LSGLNRKTTGEIIRELKIENIIYQDKRNWIIK 228
Cdd:COG0664  167 YLGLTRETVSRILKKLEKEGLIELERGRITIL 198
CAP_ED cd00038
effector domain of the CAP family of transcription factors; members include CAP (or cAMP ...
 45-133 8.81e-10

effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO sensor. In all cases binding of the effector leads to conformational changes and the ability to activate transcription. Cyclic nucleotide-binding domain similar to CAP are also present in cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) and vertebrate cyclic nucleotide-gated ion-channels. Cyclic nucleotide-monophosphate binding domain; proteins that bind cyclic nucleotides (cAMP or cGMP) share a structural domain of about 120 residues; the best studied is the prokaryotic catabolite gene activator, CAP, where such a domain is known to be composed of three alpha-helices and a distinctive eight-stranded, antiparallel beta-barrel structure; three conserved glycine residues are thought to be essential for maintenance of the structural integrity of the beta-barrel; CooA is a homodimeric transcription factor that belongs to CAP family; cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) contain two tandem copies of the cyclic nucleotide-binding domain; cAPK's are composed of two different subunits, a catalytic chain and a regulatory chain, which contains both copies of the domain; cGPK's are single chain enzymes that include the two copies of the domain in their N-terminal section; also found in vertebrate cyclic nucleotide-gated ion-channels


Pssm-ID: 237999 [Multi-domain]  Cd Length: 115  Bit Score: 54.64  E-value: 8.81e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926  45 YKKGQVIYHSTDQIKFVYFLVNGCI-LHESSNITGDNYLRLSKDENIFPMNFIFNETPAPYEIcTALTDCKILTLPKDLL 123
Cdd:cd00038   22 FPAGEVIIRQGDPADSLYIVLSGSVeVYKLDEDGREQIVGFLGPGDLFGELALLGNGPRSATV-RALTDSELLVLPRSDF 100

                         90
                 ....*....|
gi 446194926 124 EYLCRKHNEI 133
Cdd:cd00038  101 RRLLQEYPEL 110
cNMP_binding pfam00027
Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, ...
 44-130 1.23e-09

Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, oxygen and 2-oxoglutarate (Matilla et. al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).


Pssm-ID: 459637 [Multi-domain]  Cd Length: 89  Bit Score: 53.38  E-value: 1.23e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926   44 QYKKGQVIYHSTDQIKFVYFLVNGCIlhESSNITGD---NYLRLSKDENIFPMNFIFNETPAPYEIcTALTDCKILTLPK 120
Cdd:pfam00027   3 SYKAGEVIFREGDPADSLYIVLSGKV--KVYRTLEDgreQILAVLGPGDFFGELALLGGEPRSATV-VALTDSELLVIPR 79

                          90
                  ....*....|
gi 446194926  121 DLLEYLCRKH 130
Cdd:pfam00027  80 EDFLELLERD 89
HTH_CRP cd00092
helix_turn_helix, cAMP Regulatory protein C-terminus; DNA binding domain of prokaryotic ...
 160-218 1.01e-03

helix_turn_helix, cAMP Regulatory protein C-terminus; DNA binding domain of prokaryotic regulatory proteins belonging to the catabolite activator protein family.


Pssm-ID: 238044 [Multi-domain]  Cd Length: 67  Bit Score: 36.49  E-value: 1.01e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 446194926 160 AKERIERILQILCLSigddNGEFYELKQIMTVQLISNLSGLNRKTTGEIIRELKIENII 218
Cdd:cd00092    1 AKERLASFLLNLSLR----YGAGDLVQLPLTRQEIADYLGLTRETVSRTLKELEEEGLI 55
cNMP smart00100
Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a ...
 45-124 2.31e-03

Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a prokaryotic homologue of eukaryotic cNMP-binding domains, present in ion channels, and cNMP-dependent kinases.


Pssm-ID: 197516 [Multi-domain]  Cd Length: 120  Bit Score: 36.61  E-value: 2.31e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446194926    45 YKKGQVIYHSTDQIKFVYFLVNGC--ILHESSNiTGDNYLRLSKDENIFPMNFIFNETPAPYEI-CTALTDCKILTLPKD 121
Cdd:smart00100  22 YPAGEVIIRQGDVGDSFYIIVSGEveVYKVLED-GEEQIVGTLGPGDFFGELALLTNSRRAASAaAVALELATLLRIDFR 100


                   ...
gi 446194926   122 LLE 124
Cdd:smart00100 101 DFL 103
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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