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Conserved domains on  [gi|446273632|ref|WP_000351487|]
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MULTISPECIES: oxygen-insensitive NAD(P)H nitroreductase [Enterobacteriaceae]

Protein Classification

NAD(P)H-dependent oxidoreductase( domain architecture ID 10793524)

NAD(P)H-dependent oxidoreductase, similar to nitroreductase NfsB, which catalyzes the reduction of flavin or nitrocompounds using NAD(P)H as electron donor

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
1-217 6.43e-153

oxygen-insensitive NAD(P)H nitroreductase;


:

Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 422.84  E-value: 6.43e-153
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   1 MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASH 80
Cdd:PRK11053   1 MDIVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKAAAGNYAFNERKILDASH 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  81 VVVFCAKTAMDDVWLKLVVDQEDADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD 160
Cdd:PRK11053  81 VVVFCAKTDMDDAYLELVLEQEDADGRFATEEAKAAQDKGRRFFADMHRKELKDLQHWMEKQVYLALGNLLLGAAALGID 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 446273632 161 AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV 217
Cdd:PRK11053 161 ATPIEGFDAAILDAEFGLREKGLTSSVVVPLGYHSEEDFNAKLPKSRLPQETIFTEI 217
 
Name Accession Description Interval E-value
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
1-217 6.43e-153

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 422.84  E-value: 6.43e-153
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   1 MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASH 80
Cdd:PRK11053   1 MDIVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKAAAGNYAFNERKILDASH 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  81 VVVFCAKTAMDDVWLKLVVDQEDADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD 160
Cdd:PRK11053  81 VVVFCAKTDMDDAYLELVLEQEDADGRFATEEAKAAQDKGRRFFADMHRKELKDLQHWMEKQVYLALGNLLLGAAALGID 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 446273632 161 AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV 217
Cdd:PRK11053 161 ATPIEGFDAAILDAEFGLREKGLTSSVVVPLGYHSEEDFNAKLPKSRLPQETIFTEI 217
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 2-211 1.04e-64

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 197.09  E-value: 1.04e-64
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   2 DIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagnyVFNERKMLDASHV 81
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAA----WFNQPQIKDASHV 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  82 VVFCAKTamddvwlklvvdqedadgrfatpeakaandkgrkffadmhrkdlhddaEWMAKQVYLNVGNFLLGVAALGLDA 161
Cdd:cd02149   77 VVFLAKK------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDS 108

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 446273632 162 VPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEdfnaTLPKSRLPQN 211
Cdd:cd02149  109 CPIEGFDPAKLDEILGLDEKGYKISVMVAFGYRSEE----KLPKSRKPLE 154
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 3-209 5.11e-35

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 121.88  E-value: 5.11e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   3 IISVALKRHSTKAFDaSKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGnyvFNERKMLDASHVV 82
Cdd:COG0778    1 LLELLLTRRSVRKFT-DKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEALAE---ANQEWVADAPVLI 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  83 VFCAKTAMDDvwlklvvdqedadgrfatpeakaandkgrkffadmhrkdlHDDAEWMAKQVYLNVGNFLLGVAALGLDAV 162
Cdd:COG0778   77 VVCADPDRSE----------------------------------------KVPERYALLDAGIAAQNLLLAARALGLGTC 116

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 446273632 163 PIEGFDAAILDAEFGLKEkGYTSLVVVPVGHHSVEDFnatlPKSRLP 209
Cdd:COG0778  117 WIGGFDPEKVRELLGLPE-GEEPVALLALGYPAEELN----PRPRKP 158
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 7-193 3.37e-27

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 101.70  E-value: 3.37e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632    7 ALKRHSTKAFDAsKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVV---- 82
Cdd:pfam00881   1 IRQRRSVRKFDP-EPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLLLRRdanl 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   83 VFCAKTAMDDVWLKLVVDQEDADGRFATPEakaandkgrkffadmhrkdlhDDAEWMAKQVYLNVGNFLLGVAALGLDAV 162
Cdd:pfam00881  80 KLLLQDFLRGAPVLIVITASLSTYLRKAAE---------------------RAYREALLDAGAAAQNLLLAATSLGLGSC 138

                         170       180       190
                  ....*....|....*....|....*....|.
gi 446273632  163 PIEGFDAAILDAEFGLkEKGYTSLVVVPVGH 193
Cdd:pfam00881 139 PIGGFDAAAVRELLGL-PDDERLVGLIAVGY 168
 
Name Accession Description Interval E-value
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
1-217 6.43e-153

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 422.84  E-value: 6.43e-153
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   1 MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASH 80
Cdd:PRK11053   1 MDIVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKAAAGNYAFNERKILDASH 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  81 VVVFCAKTAMDDVWLKLVVDQEDADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD 160
Cdd:PRK11053  81 VVVFCAKTDMDDAYLELVLEQEDADGRFATEEAKAAQDKGRRFFADMHRKELKDLQHWMEKQVYLALGNLLLGAAALGID 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 446273632 161 AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV 217
Cdd:PRK11053 161 ATPIEGFDAAILDAEFGLREKGLTSSVVVPLGYHSEEDFNAKLPKSRLPQETIFTEI 217
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 2-211 1.04e-64

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 197.09  E-value: 1.04e-64
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   2 DIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagnyVFNERKMLDASHV 81
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAA----WFNQPQIKDASHV 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  82 VVFCAKTamddvwlklvvdqedadgrfatpeakaandkgrkffadmhrkdlhddaEWMAKQVYLNVGNFLLGVAALGLDA 161
Cdd:cd02149   77 VVFLAKK------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDS 108

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 446273632 162 VPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEdfnaTLPKSRLPQN 211
Cdd:cd02149  109 CPIEGFDPAKLDEILGLDEKGYKISVMVAFGYRSEE----KLPKSRKPLE 154
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 3-209 5.11e-35

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 121.88  E-value: 5.11e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   3 IISVALKRHSTKAFDaSKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGnyvFNERKMLDASHVV 82
Cdd:COG0778    1 LLELLLTRRSVRKFT-DKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEALAE---ANQEWVADAPVLI 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  83 VFCAKTAMDDvwlklvvdqedadgrfatpeakaandkgrkffadmhrkdlHDDAEWMAKQVYLNVGNFLLGVAALGLDAV 162
Cdd:COG0778   77 VVCADPDRSE----------------------------------------KVPERYALLDAGIAAQNLLLAARALGLGTC 116

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 446273632 163 PIEGFDAAILDAEFGLKEkGYTSLVVVPVGHHSVEDFnatlPKSRLP 209
Cdd:COG0778  117 WIGGFDPEKVRELLGLPE-GEEPVALLALGYPAEELN----PRPRKP 158
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 7-193 3.37e-27

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 101.70  E-value: 3.37e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632    7 ALKRHSTKAFDAsKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVV---- 82
Cdd:pfam00881   1 IRQRRSVRKFDP-EPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLLLRRdanl 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   83 VFCAKTAMDDVWLKLVVDQEDADGRFATPEakaandkgrkffadmhrkdlhDDAEWMAKQVYLNVGNFLLGVAALGLDAV 162
Cdd:pfam00881  80 KLLLQDFLRGAPVLIVITASLSTYLRKAAE---------------------RAYREALLDAGAAAQNLLLAATSLGLGSC 138

                         170       180       190
                  ....*....|....*....|....*....|.
gi 446273632  163 PIEGFDAAILDAEFGLkEKGYTSLVVVPVGH 193
Cdd:pfam00881 139 PIGGFDAAAVRELLGL-PDDERLVGLIAVGY 168
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 8-193 2.77e-23

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 90.82  E-value: 2.77e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   8 LKRHSTKAFDaSKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNYVFnerkMLDASHVVVFCAk 87
Cdd:cd02062    2 KTRRSIRKFT-DKPVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDREKKEKLAKLAAPNQKF----IAGAPVVIVVVA- 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  88 tamddvwlklvvdqedadgrfatpeakaandkgrkffadmhrkDLHDDAEWMAKQVYLNVGNFLLGVAALGLDAVPIEGF 167
Cdd:cd02062   76 -------------------------------------------DPDKSRPWALEDAGAAAQNLLLAAAALGLGSCWIGGF 112

                        170       180
                 ....*....|....*....|....*...
gi 446273632 168 D--AAILDAEFGLKEkGYTSLVVVPVGH 193
Cdd:cd02062  113 DfrEDKVRELLGIPE-NLRPVALIAIGY 139
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 9-211 6.24e-19

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 79.59  E-value: 6.24e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   9 KRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagnyvFNERKMLDASHVVVFCAkt 88
Cdd:cd02137    6 SRRSVRNFDPDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAA-----YNQPQVTTASAVILVLG-- 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  89 amddvwlklvvdqedadgrfatpeakaanDKGRKFFAdmhrkdlhddaewmakqvylnvGNFLLGVAALGLDAVPIEGFD 168
Cdd:cd02137   79 -----------------------------DLNAGLAA----------------------MNLMLAAKAKGYDTCPMGGFD 107

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 446273632 169 AAILDAEFGLkEKGYTSLVVVPVGHHSVEDfnatLPKSRLPQN 211
Cdd:cd02137  108 KEKVAELLNL-PDRYVPVLLIAIGKAADKA----PRSGRLPVD 145
RutE-like cd02148
nitroreductase similar to Escherichia coli RutE; A subfamily of the nitroreductase family ...
 11-193 2.18e-14

nitroreductase similar to Escherichia coli RutE; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. RutE is involved in the utilization of uracil as the sole nitrogen source; it appears to have the same function as YdfG, which reduces malonic semialdehyde to 3-hydroxypropionic acid.


Pssm-ID: 380323 [Multi-domain]  Cd Length: 186  Bit Score: 68.43  E-value: 2.18e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  11 HSTKAFDaSKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNyvfNERKMLDASHVVVFCAKTAM 90
Cdd:cd02148   10 RTHNAWE-DKPVSDEELRAIYDLAKWGPTAANCSPARIVFVRSAEAKERLVPHLSEG---NREKTMAAPVTAILAYDTEF 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  91 DDvWLklvvdqedadgrfatPEAKAANDKGRKFFADMhrkdlHDDAEWMAKQ-VYLNVGNFLLGVAALGLDAVPIEGFDA 169
Cdd:cd02148   86 YE-HL---------------PRLFPHGDARSWFFGSG-----PARAEETAFRnASLQAAYFILAARALGLDCGPMSGFDA 144

                        170       180
                 ....*....|....*....|....
gi 446273632 170 AILDAEFgLKEKGYTSLVVVPVGH 193
Cdd:cd02148  145 AGVDAEF-FAGTRWKSNLVVNLGY 167
PRK05365 PRK05365
malonic semialdehyde reductase; Provisional
 10-193 2.81e-14

malonic semialdehyde reductase; Provisional


Pssm-ID: 180040 [Multi-domain]  Cd Length: 195  Bit Score: 68.30  E-value: 2.81e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  10 RHSTKAFdASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNyvfNERKMLDASHVVVFCAKTA 89
Cdd:PRK05365  16 ARTHNGW-LDEPVSDEQLRELYDLVKWGPTSANCSPARFVFVRSAEAKERLRPALSEG---NLAKTLAAPVTAIVAYDTE 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  90 MDDVWLKLVvDQEDADGRFATPEAKAandkgrkffadmhrkdlhddaEWMAKQ-VYLNVGNFLLGVAALGLDAVPIEGFD 168
Cdd:PRK05365  92 FHEHLPKLF-PHADARSWFAGNPALA---------------------EETAFRnSSLQGAYLILAARALGLDAGPMSGFD 149

                        170       180
                 ....*....|....*....|....*
gi 446273632 169 AAILDAEFgLKEKGYTSLVVVPVGH 193
Cdd:PRK05365 150 AAAVDAEF-FAGTTWKSNFLVNIGY 173
TdsD-like cd02138
nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase ...
 8-198 4.12e-13

nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to Burkholderia pseudomallei TdsD, may be involved in the processing of organosulfur compounds. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380315 [Multi-domain]  Cd Length: 174  Bit Score: 64.87  E-value: 4.12e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   8 LKRHSTKAFDaSKKLTPEQaeqIKTLL---QYSPSSTNSQPWHFIVASTE-EGKARVAKSAAGnyvFNERKMLDASHVVV 83
Cdd:cd02138    3 AERWSPRAFS-PEPISEED---LLSLFeaaRWAPSCFNEQPWRFVVARRDtEAFEKLLDLLAE---GNQSWAKNAPVLIV 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  84 FCAKTAMDDvwlklvvdqedadgrfatpeakaaNDKGRKFFadmhrkdLHDdaewmakqVYLNVGNFLLGVAALGLDAVP 163
Cdd:cd02138   76 VLAKTEFDH------------------------NGKPNRYA-------LFD--------TGAAVANLALQATALGLVVHQ 116

                        170       180       190
                 ....*....|....*....|....*....|....*
gi 446273632 164 IEGFDAAILDAEFGLKEkGYTSLVVVPVGHHSVED 198
Cdd:cd02138  117 MAGFDPEKAKEALGIPD-EYEPITMIAIGYPGDPE 150
nitroreductase cd03370
uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus ...
 7-197 7.65e-12

uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus thermophilus NADH oxidase and other, uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380327 [Multi-domain]  Cd Length: 191  Bit Score: 61.57  E-value: 7.65e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   7 ALKRHSTKAFdASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagnyvFNERKMLDASHVVVfca 86
Cdd:cd03370    5 IESRRSIRKY-TQEPVPDEDLREILRLAGLAPSAWNIQPWRFVVVRDAELKEQLQAAA-----YGQAQVTSAPAVIV--- 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  87 ktamddvwlkLVVDQEDADGRFA-------TPEAKAANDKG-RKFFADMhrkDLHDDAEWMAKQVYLNVGNFLLGVAALG 158
Cdd:cd03370   76 ----------IYSDMEDALANLEetihpglSEERRQREAAGlRGAFGKM---SVEQRGQWGLAQANIALGFLLLAAQSLG 142

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 446273632 159 LDAVPIEGFDAAILDAEFGLKEKgYTSLVVVPVGH----------HSVE 197
Cdd:cd03370  143 YDTSPMLGFDPEKVKALLGLPEH-VTIAALVALGKpaeegyphhrHSLE 190
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 2-193 1.37e-10

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 57.40  E-value: 1.37e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   2 DIISVALKRHSTKAFDaSKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagNYVFNerkmldASHV 81
Cdd:cd20609    1 DFLELAKKRYSVRKFS-DKPVEKEKLDKILEAGRLAPTAVNYQPQRILVVRSEEALEKLAKAT--PRFFG------APLV 71

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  82 VVFCAKTamDDVWlklvvdqedadgrfatpeaKAANDKgrKFFADMhrkdlhdDA----EWMakqvylnvgnfLLGVAAL 157
Cdd:cd20609   72 IVVCYDK--DESW-------------------KRPYDG--KDSGDI-------DAaivaTHM-----------MLAATEL 110

                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 446273632 158 GLDAVPIEGFDAAILDAEFGLKEkGYTSLVVVPVGH 193
Cdd:cd20609  111 GLGTCWVGNFDPEKVREAFNLPE-NLEPVAILPLGY 145
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 6-104 1.74e-09

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 54.46  E-value: 1.74e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   6 VALKRHSTKAFDAsKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVA--KSAAGNYVfnerkmLDASHVVV 83
Cdd:cd02151    2 LLKKRRSIRKYTD-EPIEEEKLEEILEAALLAPSSRNSRPVEFIVVDDKETLKKLSecKPHGSAFL------KGAPAAIV 74

                         90       100
                 ....*....|....*....|.
gi 446273632  84 FCAKTAMDDVWLklvvdqEDA 104
Cdd:cd02151   75 VLADTEKSDTWI------EDA 89
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 6-90 2.57e-09

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 54.13  E-value: 2.57e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   6 VALKRHSTKAFDaSKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASteeGKARVAKSAAgnyvfnerkMLDASHVVVFC 85
Cdd:cd02136    1 AIKSRRSVRAFK-DKPVPKETIEKILEAARRAPSGKNTQPWRVYVVT---GKARERLKKA---------FFGAPVALFLT 67


                 ....*
gi 446273632  86 AKTAM 90
Cdd:cd02136   68 MDKVL 72
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 3-86 6.79e-09

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 52.86  E-value: 6.79e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   3 IISVALKRHSTKAFdaskKLTPEQAEQIKTLL---QYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNyvfneRKMLDAS 79
Cdd:cd02139    1 VYEAIKKRRSIRKY----KPTPVEEEKLLRILeaaRLAPSAKNRQPWRFIVVKDKELKEKLAEAANGQ-----KFIAEAP 71


                 ....*..
gi 446273632  80 HVVVFCA 86
Cdd:cd02139   72 VVIVACA 78
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 10-87 9.05e-08

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 49.64  E-value: 9.05e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  10 RHSTKAFdaSKKLTPEqaEQIKTLL---QYSPSSTNSQPWHFIVASTEEGKARVAKSAagNYVFNERKmlDASHVVVFCA 86
Cdd:cd20608    7 RRSVRRF--SDKPVEE--EKLEKILeaaRLAPSWANKQCWRFIVVTDKETLSELAKKE--SPSNGWLK--DAPVIIVVCA 78


                 .
gi 446273632  87 K 87
Cdd:cd20608   79 D 79
nitroreductase cd02150
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 8-193 1.80e-06

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380325 [Multi-domain]  Cd Length: 156  Bit Score: 46.05  E-value: 1.80e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   8 LKRHSTKAFdaSKKLTPEqaEQIKTLLQ---YSPSSTNSQPWHFIVASTEEGKARVAKsaagnyVFNERKML-DASHVVV 83
Cdd:cd02150    2 LTRRSIRKY--TDKPVEE--EDIEKLLRaamAAPSAGNQQPWHFIVVTDREKLDKIAE------AHPYGKMLkEAPLAIV 71

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  84 FCA---KTAMDDVWlklVVDqedadgrfatpeAKAAndkgrkffadmhrkdlhddAEwmakqvylnvgNFLLGVAALGLD 160
Cdd:cd02150   72 VCGdpsKEKAPGYW---VQD------------CSAA-------------------TE-----------NILLAAHALGLG 106

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 446273632 161 AV-----PIEGFDAAILDaEFGLKEkGYTSLVVVPVGH 193
Cdd:cd02150  107 AVwlgvyPFEERVKAIRE-ILNIPE-NIIPFCVIALGY 142
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 4-216 4.87e-05

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 42.99  E-value: 4.87e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   4 ISVALKRHSTKAFDAsKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagnyvFNERKMLDASHVVV 83
Cdd:cd02146    2 IETILNHRSVRKFTD-EPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELA-----GNQPYVAQAPVFLV 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632  84 FCAKTAmddvwlklvvdqedadgRFatpeAKAANDKGRKFFAdmhrkdlHDDAE-WMAKQV--YLNVGNFLLGVAALGLD 160
Cdd:cd02146   76 FCADLY-----------------RH----QKIAEEAGGKDVG-------LDYLEsFLVGVVdaALAAQNALVAAESLGLG 127

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446273632 161 AVPIEGF--DAAILDAEFGLKEKgytslvVVP-----VGHhsvEDFNATLpKSRLPQNITLTE 216
Cdd:cd02146  128 IVYIGGIrnNPEEVIELLGLPEY------VFPlfgltVGH---PDPTPEV-KPRLPLEAVVHE 180
YdjA-like cd02135
nitroreductase family protein similar to Escherichia coli YdjA; A subfamily of the ...
 4-92 7.09e-04

nitroreductase family protein similar to Escherichia coli YdjA; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase YdjA from Escherichia coli. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. Members of this family are also called NADH dehydrogenase, oxygen-insensitive NAD(P)H nitrogenase or dihydropteridine reductase.


Pssm-ID: 380312 [Multi-domain]  Cd Length: 162  Bit Score: 38.74  E-value: 7.09e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273632   4 ISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVAsTEEGKARVAKSAAGNYVFNERKM-------- 75
Cdd:cd02135    1 LELIKTRRSIRKFKLTGAPPEEQLEELLEAAMWAPNHGKLEPWRFIVV-TGEGRERLAELLAAAAAARAPGAdpekleka 79

                         90       100
                 ....*....|....*....|.
gi 446273632  76 ----LDASHVVVFCAKTAMDD 92
Cdd:cd02135   80 rekaLRAPVVIAVVAKPDEDP 100
McbC_SagB-like_oxidoreductase cd02142
oxidase similar to the microcin B17 processing protein McbC; This family is the oxidase domain ...
 148-193 1.46e-03

oxidase similar to the microcin B17 processing protein McbC; This family is the oxidase domain of NRPS (non-ribosomal peptide synthetase) and other systems that modify polypeptides by cyclizing a thioester to form a ring. These include EpoB, part of the epothilone biosynthesis pathway; TubD, part of the tubulysin biosynthesis pathway, MtsD, part of the myxothiozol biosynthesis pathway; IndC, part of the indigoidine biosynthesis pathway and TfxB, part of the trifitoxin processing pathway. All are FMN-dependent and oxidize the product of the cyclization of thioesters in short polypeptides.


Pssm-ID: 380318  Cd Length: 200  Bit Score: 38.17  E-value: 1.46e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 446273632 148 GNFLLGVAALGLDAVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGH 193
Cdd:cd02142  155 QNLYLAATALGLGLCAIGAFDDDALRELLGLDEVEEVVLYAFVVGG 200
iodotyrosine_dehalogenase cd02144
iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the ...
 9-75 3.16e-03

iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the 3, 5 positions of L-tyosine in thyroid, liver and kidney, using NADPH as electron donor. This enzyme is a homolog of the nitroreductase family. These enzymes are usually homodimers.


Pssm-ID: 380320  Cd Length: 192  Bit Score: 37.13  E-value: 3.16e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446273632   9 KRHSTKAFdaskKLTPEQAEQIKTLLQ---YSPSSTNSQPWHFIVASTEEGKARVAKSA-AGNYVFNERKM 75
Cdd:cd02144    7 KRRSVRDF----SSEPVPREVIENAIRtagTAPSGANTQPWTFVVVSDPEIKRKIREAAeEEEKEFYEKRM 73
TM1586_NiRdase pfam14512
Putative TM nitroreductase; Compared with the more traditional NADH oxidase/flavin reductase ...
 8-72 9.43e-03

Putative TM nitroreductase; Compared with the more traditional NADH oxidase/flavin reductase family, this family is a duplication, consisting of two similar domains arranged as the subunits of the dimeric NADH oxidase/flavin reductase with one conserved active site.


Pssm-ID: 405236 [Multi-domain]  Cd Length: 214  Bit Score: 36.12  E-value: 9.43e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446273632    8 LKRHSTKAFDASKKL----TPEQAEQIKTLLQYSPSSTNSQPWHFIVasteEGKARVAKSAAGNYVFNE 72
Cdd:pfam14512 136 GVRAKRKPLDELCVVkedeLPEWFKEGMEAARLAPSAMNQQPWRFVL----VDDNRIHAFASVIGFFSK 200
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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