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Conserved domains on  [gi|446281531|ref|WP_000359386|]
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Spy0128 family protein, partial [Streptococcus mitis]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 1201-1300 1.83e-27

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


:

Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 107.87  E-value: 1.83e-27
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  1201 TLTGRELKADEFEFTLTDQAGTLKETVKNDKDGNVKFSALEFDKAGTFTYKIAEKAGTATGIQYDTKTITATVTVADNGK 1280
Cdd:pfam12892    6 TLTGRDLKDGEFTFTLTDEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGGVPGVTYDTHTYTVTVTVTDNGD 85

                           90       100
                   ....*....|....*....|..
gi 446281531  1281 GVLEATVAYDDEKA--FKNTYT 1300
Cdd:pfam12892   86 GTLTATVTYDDGTEltFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 840-952 8.38e-25

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


:

Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 100.17  E-value: 8.38e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531   840 LEVTKQLTGRTtgAQENEFEFTLTDQVGNV-EKAKNGADGKVKFSELTFDEAGTYTYTIKEVKAGttENGITYDSKTVTA 918
Cdd:pfam12892    1 LKATKTLTGRD--LKDGEFTFTLTDEDGNViQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYTV 76

                           90       100       110
                   ....*....|....*....|....*....|....
gi 446281531   919 KVTVTDDGQGKLHATVEYSSDGTAnstTFTNTYN 952
Cdd:pfam12892   77 TVTVTDNGDGTLTATVTYDDGTEL---TFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 1081-1188 2.03e-23

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


:

Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 96.32  E-value: 2.03e-23
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  1081 LEVMKTLSGRELKADEFDFVLKnDADGSELQKVKNTADGKVTFAPIEYTKAGTYNYTIVETNAGktVNGVTYDNLEVKVT 1160
Cdd:pfam12892    1 LKATKTLTGRDLKDGEFTFTLT-DEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYTVT 77

                           90       100       110
                   ....*....|....*....|....*....|
gi 446281531  1161 VEVTDDSEGKLHANVTYSADKE--FNNSYG 1188
Cdd:pfam12892   78 VTVTDNGDGTLTATVTYDDGTEltFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 976-1073 1.29e-20

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


:

Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 88.23  E-value: 1.29e-20
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531   976 PLKGGEFVFSLINHEGDPVEKTTNDADGNIKFEDLEFNKADTYHYTIVEKNAGttDKGITYSNKTIEVTIKVVDNGNGAL 1055
Cdd:pfam12892   11 DLKDGEFTFTLTDEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYTVTVTVTDNGDGTL 88

                           90
                   ....*....|....*....
gi 446281531  1056 EATVSYDNNDS-TFENTYK 1073
Cdd:pfam12892   89 TATVTYDDGTElTFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 688-786 1.46e-18

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


:

Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 82.45  E-value: 1.46e-18
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531   688 IEVKKELTGgrpTPLQNEEFEFILKDKNGQEVQRAKNDAAGRVVFKDIPFDKAGEYEFTVVEVNAGqtIDGVTHDSRKVP 767
Cdd:pfam12892    1 LKATKTLTG---RDLKDGEFTFTLTDEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYT 75

                           90
                   ....*....|....*....
gi 446281531   768 VTVSVTDDGKGKLVATVLY 786
Cdd:pfam12892   76 VTVTVTDNGDGTLTATVTY 94
ClfA COG4932
Clumping factor A-related surface protein, MSCRAMM (microbial surface components recognizing ...
 352-695 1.15e-09

Clumping factor A-related surface protein, MSCRAMM (microbial surface components recognizing adhesive matrix molecules) family, DEv-IgG fold [Cell wall/membrane/envelope biogenesis];


:

Pssm-ID: 443959 [Multi-domain]  Cd Length: 689  Bit Score: 62.68  E-value: 1.15e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  352 KTVKSVGASVTADIAGKIKITKYDEADATVKLAGVVFEIrEKTTNNLVDTVTTDADGIAVSKALNNGKYIVKEKTPKSGY 431
Cdd:COG4932   343 TTTVTVTNGNNEVKTGSVTLTKVDADDGEAPLAGAEFTL-TDADGTVVATITTDADGTASFKGLAPGTYTLTETKAPEGY 421

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  432 QVNSQEFEVEMKDG-KGVPLSISNKRVTVDFEATKTWVNGKATDYKEVKLGLYVHKEGQTVADAKPVSGNYTPEVTKSNG 510
Cdd:COG4932   422 TLDSTPITVTVTDGgTGAIDTITNERKKGSVQVTKVDAPLAGATFTLTDADGTVVTLTTDADLAGATFEADGKVVTTTDA 501

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  511 VYTYKWKNQLPKYDVDGTTELIYSVRELQDQTNIPLDETETVVGGTSDYVVSYNEDKTKITNTYKPAKISVTAKKVWVGG 590
Cdd:COG4932   502 SGKYTFKNLPPGTYTDAGGSATVITDDTDGTVGDEATGTDPEVTVTGKSTTTTPDVALLTNLGTTEDALTSLAKTGDEVG 581

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  591 QEHIRPTVYFKLYRTPEGGAIEEVAGAEQKEVPKTNGTLEWPDLPATDEHGVKYTYSVMEVDEDGNLIVDTIDGYTPEQT 670
Cdd:COG4932   582 KGLTLTTTTTVDTLDTNATEKTETVTVTAQLIGVKTTKLTDTTDPKGGTVEEATTTGGTANTGKTGTDLTDDTTVTSTTN 661

                         330       340
                  ....*....|....*....|....*
gi 446281531  671 ADLTVTNTYSTSPTKADIEVKKELT 695
Cdd:COG4932   662 TATSVEDTTDVDNKDAFTGGTEPGG 686
Big_8 super family cl40244
Bacterial Ig domain; This entry represents a bacterial Ig-fold domain that is found in a wide ...
 65-144 3.08e-08

Bacterial Ig domain; This entry represents a bacterial Ig-fold domain that is found in a wide range of bacterial cell surface adherence proteins.


The actual alignment was detected with superfamily member pfam17961:

Pssm-ID: 465589 [Multi-domain]  Cd Length: 102  Bit Score: 52.61  E-value: 3.08e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531    65 KLKYSGKFSFPNvaanEIKDGDYFIVKAPDNLSL-----TDGSLDLIDSTSNIkMGTVRVDNANHQLVFTFNDKVKDKQN 139
Cdd:pfam17961    7 SLKLKADFSLGD----SVKEGDYFTIKLPDNLKFygintSDKSFDIKDDNGEV-IAKGTYDPGTGTITYTFTDYVENKSN 81


                   ....*
gi 446281531   140 IRGDF 144
Cdd:pfam17961   82 IKGSL 86
 
Name Accession Description Interval E-value
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 1201-1300 1.83e-27

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 107.87  E-value: 1.83e-27
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  1201 TLTGRELKADEFEFTLTDQAGTLKETVKNDKDGNVKFSALEFDKAGTFTYKIAEKAGTATGIQYDTKTITATVTVADNGK 1280
Cdd:pfam12892    6 TLTGRDLKDGEFTFTLTDEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGGVPGVTYDTHTYTVTVTVTDNGD 85

                           90       100
                   ....*....|....*....|..
gi 446281531  1281 GVLEATVAYDDEKA--FKNTYT 1300
Cdd:pfam12892   86 GTLTATVTYDDGTEltFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 840-952 8.38e-25

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 100.17  E-value: 8.38e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531   840 LEVTKQLTGRTtgAQENEFEFTLTDQVGNV-EKAKNGADGKVKFSELTFDEAGTYTYTIKEVKAGttENGITYDSKTVTA 918
Cdd:pfam12892    1 LKATKTLTGRD--LKDGEFTFTLTDEDGNViQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYTV 76

                           90       100       110
                   ....*....|....*....|....*....|....
gi 446281531   919 KVTVTDDGQGKLHATVEYSSDGTAnstTFTNTYN 952
Cdd:pfam12892   77 TVTVTDNGDGTLTATVTYDDGTEL---TFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 1081-1188 2.03e-23

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 96.32  E-value: 2.03e-23
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  1081 LEVMKTLSGRELKADEFDFVLKnDADGSELQKVKNTADGKVTFAPIEYTKAGTYNYTIVETNAGktVNGVTYDNLEVKVT 1160
Cdd:pfam12892    1 LKATKTLTGRDLKDGEFTFTLT-DEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYTVT 77

                           90       100       110
                   ....*....|....*....|....*....|
gi 446281531  1161 VEVTDDSEGKLHANVTYSADKE--FNNSYG 1188
Cdd:pfam12892   78 VTVTDNGDGTLTATVTYDDGTEltFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 976-1073 1.29e-20

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 88.23  E-value: 1.29e-20
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531   976 PLKGGEFVFSLINHEGDPVEKTTNDADGNIKFEDLEFNKADTYHYTIVEKNAGttDKGITYSNKTIEVTIKVVDNGNGAL 1055
Cdd:pfam12892   11 DLKDGEFTFTLTDEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYTVTVTVTDNGDGTL 88

                           90
                   ....*....|....*....
gi 446281531  1056 EATVSYDNNDS-TFENTYK 1073
Cdd:pfam12892   89 TATVTYDDGTElTFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 688-786 1.46e-18

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 82.45  E-value: 1.46e-18
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531   688 IEVKKELTGgrpTPLQNEEFEFILKDKNGQEVQRAKNDAAGRVVFKDIPFDKAGEYEFTVVEVNAGqtIDGVTHDSRKVP 767
Cdd:pfam12892    1 LKATKTLTG---RDLKDGEFTFTLTDEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYT 75

                           90
                   ....*....|....*....
gi 446281531   768 VTVSVTDDGKGKLVATVLY 786
Cdd:pfam12892   76 VTVTVTDNGDGTLTATVTY 94
strep_pil_rpt TIGR03786
streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once ...
 882-948 3.85e-15

streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once in the major pilin of Streptococcus pyogenes, Spy0128, but in higher copy numbers in other streptococcal proteins. The domain occurs nine times in a surface-anchored protein of Bifidobacterium longum. All members of this family have LPXTG-type sortase target sequences. The S. pyogenes major pilin has been shown to undergo isopeptide bond cross-linking, mediated by sortases, that are critical to maintaining pilus structural integrity. One such Lys-to-Asn isopeptide bond is to a near-invariant Asn near the C-terminal end of this domain (column 81 of the seed alignment). A Glu in the S. pyogenes major pilin (column 25 of the seed alignment), invariant as Glu or Gln, is described as catalytic for isopeptide bond formation.


Pssm-ID: 274781 [Multi-domain]  Cd Length: 63  Bit Score: 71.18  E-value: 3.85e-15
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446281531   882 FSELTFDEAGTYTYTIKEVKagTTENGITYDSKTVTAKVTVTDDGQGKLHATVEYSSdgTANSTTFT 948
Cdd:TIGR03786    1 FSPLTFTKVGTYTYTVTEVK--GKEPGVTYDTTVHTVTVTVTDDEQGKLVATVIYDK--EKNPITFT 63
strep_pil_rpt TIGR03786
streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once ...
 1237-1293 1.09e-14

streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once in the major pilin of Streptococcus pyogenes, Spy0128, but in higher copy numbers in other streptococcal proteins. The domain occurs nine times in a surface-anchored protein of Bifidobacterium longum. All members of this family have LPXTG-type sortase target sequences. The S. pyogenes major pilin has been shown to undergo isopeptide bond cross-linking, mediated by sortases, that are critical to maintaining pilus structural integrity. One such Lys-to-Asn isopeptide bond is to a near-invariant Asn near the C-terminal end of this domain (column 81 of the seed alignment). A Glu in the S. pyogenes major pilin (column 25 of the seed alignment), invariant as Glu or Gln, is described as catalytic for isopeptide bond formation.


Pssm-ID: 274781 [Multi-domain]  Cd Length: 63  Bit Score: 69.64  E-value: 1.09e-14
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 446281531  1237 FSALEFDKAGTFTYKIAEKAGTATGIQYDTKTITATVTVADNGKGVLEATVAYDDEK 1293
Cdd:TIGR03786    1 FSPLTFTKVGTYTYTVTEVKGKEPGVTYDTTVHTVTVTVTDDEQGKLVATVIYDKEK 57
strep_pil_rpt TIGR03786
streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once ...
 1123-1182 7.76e-11

streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once in the major pilin of Streptococcus pyogenes, Spy0128, but in higher copy numbers in other streptococcal proteins. The domain occurs nine times in a surface-anchored protein of Bifidobacterium longum. All members of this family have LPXTG-type sortase target sequences. The S. pyogenes major pilin has been shown to undergo isopeptide bond cross-linking, mediated by sortases, that are critical to maintaining pilus structural integrity. One such Lys-to-Asn isopeptide bond is to a near-invariant Asn near the C-terminal end of this domain (column 81 of the seed alignment). A Glu in the S. pyogenes major pilin (column 25 of the seed alignment), invariant as Glu or Gln, is described as catalytic for isopeptide bond formation.


Pssm-ID: 274781 [Multi-domain]  Cd Length: 63  Bit Score: 58.85  E-value: 7.76e-11
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  1123 FAPIEYTKAGTYNYTIVETNAgkTVNGVTYDNLEVKVTVEVTDDSEGKLHANVTYSADKE 1182
Cdd:TIGR03786    1 FSPLTFTKVGTYTYTVTEVKG--KEPGVTYDTTVHTVTVTVTDDEQGKLVATVIYDKEKN 58
strep_pil_rpt TIGR03786
streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once ...
 1007-1066 9.81e-11

streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once in the major pilin of Streptococcus pyogenes, Spy0128, but in higher copy numbers in other streptococcal proteins. The domain occurs nine times in a surface-anchored protein of Bifidobacterium longum. All members of this family have LPXTG-type sortase target sequences. The S. pyogenes major pilin has been shown to undergo isopeptide bond cross-linking, mediated by sortases, that are critical to maintaining pilus structural integrity. One such Lys-to-Asn isopeptide bond is to a near-invariant Asn near the C-terminal end of this domain (column 81 of the seed alignment). A Glu in the S. pyogenes major pilin (column 25 of the seed alignment), invariant as Glu or Gln, is described as catalytic for isopeptide bond formation.


Pssm-ID: 274781 [Multi-domain]  Cd Length: 63  Bit Score: 58.47  E-value: 9.81e-11
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  1007 FEDLEFNKADTYHYTIVEKNagTTDKGITYSNKTIEVTIKVVDNGNGALEATVSYDNNDS 1066
Cdd:TIGR03786    1 FSPLTFTKVGTYTYTVTEVK--GKEPGVTYDTTVHTVTVTVTDDEQGKLVATVIYDKEKN 58
ClfA COG4932
Clumping factor A-related surface protein, MSCRAMM (microbial surface components recognizing ...
 352-695 1.15e-09

Clumping factor A-related surface protein, MSCRAMM (microbial surface components recognizing adhesive matrix molecules) family, DEv-IgG fold [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 443959 [Multi-domain]  Cd Length: 689  Bit Score: 62.68  E-value: 1.15e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  352 KTVKSVGASVTADIAGKIKITKYDEADATVKLAGVVFEIrEKTTNNLVDTVTTDADGIAVSKALNNGKYIVKEKTPKSGY 431
Cdd:COG4932   343 TTTVTVTNGNNEVKTGSVTLTKVDADDGEAPLAGAEFTL-TDADGTVVATITTDADGTASFKGLAPGTYTLTETKAPEGY 421

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  432 QVNSQEFEVEMKDG-KGVPLSISNKRVTVDFEATKTWVNGKATDYKEVKLGLYVHKEGQTVADAKPVSGNYTPEVTKSNG 510
Cdd:COG4932   422 TLDSTPITVTVTDGgTGAIDTITNERKKGSVQVTKVDAPLAGATFTLTDADGTVVTLTTDADLAGATFEADGKVVTTTDA 501

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  511 VYTYKWKNQLPKYDVDGTTELIYSVRELQDQTNIPLDETETVVGGTSDYVVSYNEDKTKITNTYKPAKISVTAKKVWVGG 590
Cdd:COG4932   502 SGKYTFKNLPPGTYTDAGGSATVITDDTDGTVGDEATGTDPEVTVTGKSTTTTPDVALLTNLGTTEDALTSLAKTGDEVG 581

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  591 QEHIRPTVYFKLYRTPEGGAIEEVAGAEQKEVPKTNGTLEWPDLPATDEHGVKYTYSVMEVDEDGNLIVDTIDGYTPEQT 670
Cdd:COG4932   582 KGLTLTTTTTVDTLDTNATEKTETVTVTAQLIGVKTTKLTDTTDPKGGTVEEATTTGGTANTGKTGTDLTDDTTVTSTTN 661

                         330       340
                  ....*....|....*....|....*
gi 446281531  671 ADLTVTNTYSTSPTKADIEVKKELT 695
Cdd:COG4932   662 TATSVEDTTDVDNKDAFTGGTEPGG 686
SpaA pfam17802
Prealbumin-like fold domain; This entry contains a prealbumin-like domain from a wide variety ...
 378-446 1.59e-09

Prealbumin-like fold domain; This entry contains a prealbumin-like domain from a wide variety of bacterial surface proteins. This entry corresponds to domain 1 and domain 3 of SpaA from Corynebacterium diphtheriae. Some members of this family contain an isopeptide bond.


Pssm-ID: 465513 [Multi-domain]  Cd Length: 72  Bit Score: 55.28  E-value: 1.59e-09
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446281531   378 DATVKLAGVVFEIR---EKTTNNLVDTVTTDADGIAVSKALNNGKYIVKEKTPKSGYQVNSQEFEVEMKDGK 446
Cdd:pfam17802    1 DTGKPLAGAEFTLYdadGTVDGKVVGTLTTDEDGKATFDGLPPGTYTLKETKAPDGYVLDDTPIEFTVTEDG 72
Big_8 pfam17961
Bacterial Ig domain; This entry represents a bacterial Ig-fold domain that is found in a wide ...
 65-144 3.08e-08

Bacterial Ig domain; This entry represents a bacterial Ig-fold domain that is found in a wide range of bacterial cell surface adherence proteins.


Pssm-ID: 465589 [Multi-domain]  Cd Length: 102  Bit Score: 52.61  E-value: 3.08e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531    65 KLKYSGKFSFPNvaanEIKDGDYFIVKAPDNLSL-----TDGSLDLIDSTSNIkMGTVRVDNANHQLVFTFNDKVKDKQN 139
Cdd:pfam17961    7 SLKLKADFSLGD----SVKEGDYFTIKLPDNLKFygintSDKSFDIKDDNGEV-IAKGTYDPGTGTITYTFTDYVENKSN 81


                   ....*
gi 446281531   140 IRGDF 144
Cdd:pfam17961   82 IKGSL 86
strep_pil_rpt TIGR03786
streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once ...
 732-786 1.80e-07

streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once in the major pilin of Streptococcus pyogenes, Spy0128, but in higher copy numbers in other streptococcal proteins. The domain occurs nine times in a surface-anchored protein of Bifidobacterium longum. All members of this family have LPXTG-type sortase target sequences. The S. pyogenes major pilin has been shown to undergo isopeptide bond cross-linking, mediated by sortases, that are critical to maintaining pilus structural integrity. One such Lys-to-Asn isopeptide bond is to a near-invariant Asn near the C-terminal end of this domain (column 81 of the seed alignment). A Glu in the S. pyogenes major pilin (column 25 of the seed alignment), invariant as Glu or Gln, is described as catalytic for isopeptide bond formation.


Pssm-ID: 274781 [Multi-domain]  Cd Length: 63  Bit Score: 49.22  E-value: 1.80e-07
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*
gi 446281531   732 FKDIPFDKAGEYEFTVVEVNagQTIDGVTHDSRKVPVTVSVTDDGKGKLVATVLY 786
Cdd:TIGR03786    1 FSPLTFTKVGTYTYTVTEVK--GKEPGVTYDTTVHTVTVTVTDDEQGKLVATVIY 53
CollagenBindB cd00222
Repeat unit of collagen-binding protein domain B; The collagen-binding protein mediates ...
 579-679 1.91e-06

Repeat unit of collagen-binding protein domain B; The collagen-binding protein mediates bacterial adherence to collagen; the primary sequence has a non-repetitive, collagen-binding A region, followed by instances of this B region repetitive unit. The B region has one to four 23 kDa repeat units (B1-B4), which have been suggested to serve as 'stalks' that project the A region from the bacterial surface and thus facilitate bacterial adherence to collagen. Each B repeat unit has two highly similar domains (D1 and D2) placed side-by-side; both D1 and D2 are included in this model. They exhibit a unique inverse IgG-like domain fold.


Pssm-ID: 212461 [Multi-domain]  Cd Length: 92  Bit Score: 47.29  E-value: 1.91e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  579 ISVTAKKVWVGG--QEHIRPT-VYFKLYRTPEggaieeVAGAEQKEVPKTNGTLEWPDLPATDEHGVKYTYSVMEVDedg 655
Cdd:cd00222     1 TDITVTKTWDDDdnQDGKRPEsITVQLLANGK------ETGDTVTLTASNDWTYTFTNLPKYDENGKEITYTVKEVP--- 71

                          90       100
                  ....*....|....*....|....*..
gi 446281531  656 nlivdtIDGYTPEQTAD---LTVTNTY 679
Cdd:cd00222    72 ------VPGYTTTVTGDdggFTITNTH 92
ClfA COG4932
Clumping factor A-related surface protein, MSCRAMM (microbial surface components recognizing ...
 545-1291 1.07e-05

Clumping factor A-related surface protein, MSCRAMM (microbial surface components recognizing adhesive matrix molecules) family, DEv-IgG fold [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 443959 [Multi-domain]  Cd Length: 689  Bit Score: 49.97  E-value: 1.07e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  545 PLDETETVVGGTSDYVVSYNEDKTKITNTYKPAKISVTAKKVWVGGQEHIRPTVYFKLYRTPEGGAIEEVAGAEQKEVPK 624
Cdd:COG4932    23 SLVAVTTGAVLGLALGSTGGTAGSAAAINSAPATGTATGTSAGATAVIVIAAGLTATPTETASGLGGDATVTTTANVAKV 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  625 TNGTLEWPDLPATDEHGVKYTYSVMEVDEDGNLIVDTIDGYTPEQTADLTVTNTYSTSPTKADIEVKKELTGGRPTPLQN 704
Cdd:COG4932   103 TNGAAANLTVNADGTASNAGTLAKGAETATGNLDGDAGDVTVTAAATDGVNDVDGNGASVTDSVTLKKVDDGDTGKPLPG 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  705 EEFEfiLKDKNGQEVQRAKNDAAGRVVFKDIPfdkAGEYEFTVVEVNAGQTIDGVTHDSRKVPVTVsvtddgkgklvatv 784
Cdd:COG4932   183 ATFT--LYDSDGTLVKTVTTDADGKYTFTDLP---PGTYTLTETKAPEGYVLDTKDPTGATITVTV-------------- 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  785 lyhpitavalpsanlassapgsnlvppltgavnraTDAGIQTFKNTYKATNAKAVLEVTKQLTgrTTGAQENEFEFTLTD 864
Cdd:COG4932   244 -----------------------------------NAGGTVTVTLKNTPKYTKGSVTVTKTDA--DTGEPLAGATFTLTD 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  865 QVGNVEKAKN----GADGKVKFSELtfdEAGTYTYTIKEVKAGttengitYDSKTVTAKVTVTDdgqgklhatveyssdG 940
Cdd:COG4932   287 ADGNTVVTTTvtvtDADGSYTFTDL---PPGTYTVTETKAPAG-------YDLDGEAVKVTITA---------------G 341

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  941 TANSTTFTNTYNPAKvKAPVSATKsfiNKNTNTPMPLKGGEFvfSLINHEGDPVEKTTNDADGNIKFEDLEFNKadtyhY 1020
Cdd:COG4932   342 QTTTVTVTNGNNEVK-TGSVTLTK---VDADDGEAPLAGAEF--TLTDADGTVVATITTDADGTASFKGLAPGT-----Y 410

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531 1021 TIVEKNAGTtdkGITYSNKTIEVTIKvvdnGNGALEATVSYDNNDSTFENTYKADNAKAGLEVMKTLSGRELKADEFDFV 1100
Cdd:COG4932   411 TLTETKAPE---GYTLDSTPITVTVT----DGGTGAIDTITNERKKGSVQVTKVDAPLAGATFTLTDADGTVVTLTTDAD 483

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531 1101 LKNDADGSELQKVKNT-ADGKVTF---APIEYTKAGTYNYTIVETNAGKTVNGVTYDNLEVKVTVEVTDDSEGKLHANVT 1176
Cdd:COG4932   484 LAGATFEADGKVVTTTdASGKYTFknlPPGTYTDAGGSATVITDDTDGTVGDEATGTDPEVTVTGKSTTTTPDVALLTNL 563

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531 1177 YSADKEFNNSYGASKAKAALAVKKTLTGRELKADEFEFTLTDQAGTLKETVKNDKDGNVKFSALEFDKAGTFTYKIAEKA 1256
Cdd:COG4932   564 GTTEDALTSLAKTGDEVGKGLTLTTTTTVDTLDTNATEKTETVTVTAQLIGVKTTKLTDTTDPKGGTVEEATTTGGTANT 643

                         730       740       750
                  ....*....|....*....|....*....|....*
gi 446281531 1257 GTATGIQYDTKTITATVTVADNGKGVLEATVAYDD 1291
Cdd:COG4932   644 GKTGTDLTDDTTVTSTTNTATSVEDTTDVDNKDAF 678
 
Name Accession Description Interval E-value
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 1201-1300 1.83e-27

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 107.87  E-value: 1.83e-27
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  1201 TLTGRELKADEFEFTLTDQAGTLKETVKNDKDGNVKFSALEFDKAGTFTYKIAEKAGTATGIQYDTKTITATVTVADNGK 1280
Cdd:pfam12892    6 TLTGRDLKDGEFTFTLTDEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGGVPGVTYDTHTYTVTVTVTDNGD 85

                           90       100
                   ....*....|....*....|..
gi 446281531  1281 GVLEATVAYDDEKA--FKNTYT 1300
Cdd:pfam12892   86 GTLTATVTYDDGTEltFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 840-952 8.38e-25

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 100.17  E-value: 8.38e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531   840 LEVTKQLTGRTtgAQENEFEFTLTDQVGNV-EKAKNGADGKVKFSELTFDEAGTYTYTIKEVKAGttENGITYDSKTVTA 918
Cdd:pfam12892    1 LKATKTLTGRD--LKDGEFTFTLTDEDGNViQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYTV 76

                           90       100       110
                   ....*....|....*....|....*....|....
gi 446281531   919 KVTVTDDGQGKLHATVEYSSDGTAnstTFTNTYN 952
Cdd:pfam12892   77 TVTVTDNGDGTLTATVTYDDGTEL---TFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 1081-1188 2.03e-23

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 96.32  E-value: 2.03e-23
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  1081 LEVMKTLSGRELKADEFDFVLKnDADGSELQKVKNTADGKVTFAPIEYTKAGTYNYTIVETNAGktVNGVTYDNLEVKVT 1160
Cdd:pfam12892    1 LKATKTLTGRDLKDGEFTFTLT-DEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYTVT 77

                           90       100       110
                   ....*....|....*....|....*....|
gi 446281531  1161 VEVTDDSEGKLHANVTYSADKE--FNNSYG 1188
Cdd:pfam12892   78 VTVTDNGDGTLTATVTYDDGTEltFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 976-1073 1.29e-20

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 88.23  E-value: 1.29e-20
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531   976 PLKGGEFVFSLINHEGDPVEKTTNDADGNIKFEDLEFNKADTYHYTIVEKNAGttDKGITYSNKTIEVTIKVVDNGNGAL 1055
Cdd:pfam12892   11 DLKDGEFTFTLTDEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYTVTVTVTDNGDGTL 88

                           90
                   ....*....|....*....
gi 446281531  1056 EATVSYDNNDS-TFENTYK 1073
Cdd:pfam12892   89 TATVTYDDGTElTFTNTYK 107
FctA pfam12892
Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of ...
 688-786 1.46e-18

Spy0128-like isopeptide containing domain; The FCT and equivalent region genes of Streptococcus pyogenes and other related bacteria encode surface proteins that include fibronectin- and collagen-binding proteins and the serological markers known as T antigens. Some of these proteins give rise to pilus-like appendages. The FctA family is found in many Firmicutes and related bacteria. In S. pyogenes, the pili have a role in bacterial adherence and colonization of human tissues. Members of this family have a conserved N-terminal lysine and C-terminal asparagine that can form a covalent isopeptide bond.


Pssm-ID: 463742 [Multi-domain]  Cd Length: 107  Bit Score: 82.45  E-value: 1.46e-18
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531   688 IEVKKELTGgrpTPLQNEEFEFILKDKNGQEVQRAKNDAAGRVVFKDIPFDKAGEYEFTVVEVNAGqtIDGVTHDSRKVP 767
Cdd:pfam12892    1 LKATKTLTG---RDLKDGEFTFTLTDEDGNVIQTAKNDADGTVTFGDITYTKAGTYTYTVTEVNGG--VPGVTYDTHTYT 75

                           90
                   ....*....|....*....
gi 446281531   768 VTVSVTDDGKGKLVATVLY 786
Cdd:pfam12892   76 VTVTVTDNGDGTLTATVTY 94
strep_pil_rpt TIGR03786
streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once ...
 882-948 3.85e-15

streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once in the major pilin of Streptococcus pyogenes, Spy0128, but in higher copy numbers in other streptococcal proteins. The domain occurs nine times in a surface-anchored protein of Bifidobacterium longum. All members of this family have LPXTG-type sortase target sequences. The S. pyogenes major pilin has been shown to undergo isopeptide bond cross-linking, mediated by sortases, that are critical to maintaining pilus structural integrity. One such Lys-to-Asn isopeptide bond is to a near-invariant Asn near the C-terminal end of this domain (column 81 of the seed alignment). A Glu in the S. pyogenes major pilin (column 25 of the seed alignment), invariant as Glu or Gln, is described as catalytic for isopeptide bond formation.


Pssm-ID: 274781 [Multi-domain]  Cd Length: 63  Bit Score: 71.18  E-value: 3.85e-15
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446281531   882 FSELTFDEAGTYTYTIKEVKagTTENGITYDSKTVTAKVTVTDDGQGKLHATVEYSSdgTANSTTFT 948
Cdd:TIGR03786    1 FSPLTFTKVGTYTYTVTEVK--GKEPGVTYDTTVHTVTVTVTDDEQGKLVATVIYDK--EKNPITFT 63
strep_pil_rpt TIGR03786
streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once ...
 1237-1293 1.09e-14

streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once in the major pilin of Streptococcus pyogenes, Spy0128, but in higher copy numbers in other streptococcal proteins. The domain occurs nine times in a surface-anchored protein of Bifidobacterium longum. All members of this family have LPXTG-type sortase target sequences. The S. pyogenes major pilin has been shown to undergo isopeptide bond cross-linking, mediated by sortases, that are critical to maintaining pilus structural integrity. One such Lys-to-Asn isopeptide bond is to a near-invariant Asn near the C-terminal end of this domain (column 81 of the seed alignment). A Glu in the S. pyogenes major pilin (column 25 of the seed alignment), invariant as Glu or Gln, is described as catalytic for isopeptide bond formation.


Pssm-ID: 274781 [Multi-domain]  Cd Length: 63  Bit Score: 69.64  E-value: 1.09e-14
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 446281531  1237 FSALEFDKAGTFTYKIAEKAGTATGIQYDTKTITATVTVADNGKGVLEATVAYDDEK 1293
Cdd:TIGR03786    1 FSPLTFTKVGTYTYTVTEVKGKEPGVTYDTTVHTVTVTVTDDEQGKLVATVIYDKEK 57
strep_pil_rpt TIGR03786
streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once ...
 1123-1182 7.76e-11

streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once in the major pilin of Streptococcus pyogenes, Spy0128, but in higher copy numbers in other streptococcal proteins. The domain occurs nine times in a surface-anchored protein of Bifidobacterium longum. All members of this family have LPXTG-type sortase target sequences. The S. pyogenes major pilin has been shown to undergo isopeptide bond cross-linking, mediated by sortases, that are critical to maintaining pilus structural integrity. One such Lys-to-Asn isopeptide bond is to a near-invariant Asn near the C-terminal end of this domain (column 81 of the seed alignment). A Glu in the S. pyogenes major pilin (column 25 of the seed alignment), invariant as Glu or Gln, is described as catalytic for isopeptide bond formation.


Pssm-ID: 274781 [Multi-domain]  Cd Length: 63  Bit Score: 58.85  E-value: 7.76e-11
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  1123 FAPIEYTKAGTYNYTIVETNAgkTVNGVTYDNLEVKVTVEVTDDSEGKLHANVTYSADKE 1182
Cdd:TIGR03786    1 FSPLTFTKVGTYTYTVTEVKG--KEPGVTYDTTVHTVTVTVTDDEQGKLVATVIYDKEKN 58
strep_pil_rpt TIGR03786
streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once ...
 1007-1066 9.81e-11

streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once in the major pilin of Streptococcus pyogenes, Spy0128, but in higher copy numbers in other streptococcal proteins. The domain occurs nine times in a surface-anchored protein of Bifidobacterium longum. All members of this family have LPXTG-type sortase target sequences. The S. pyogenes major pilin has been shown to undergo isopeptide bond cross-linking, mediated by sortases, that are critical to maintaining pilus structural integrity. One such Lys-to-Asn isopeptide bond is to a near-invariant Asn near the C-terminal end of this domain (column 81 of the seed alignment). A Glu in the S. pyogenes major pilin (column 25 of the seed alignment), invariant as Glu or Gln, is described as catalytic for isopeptide bond formation.


Pssm-ID: 274781 [Multi-domain]  Cd Length: 63  Bit Score: 58.47  E-value: 9.81e-11
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  1007 FEDLEFNKADTYHYTIVEKNagTTDKGITYSNKTIEVTIKVVDNGNGALEATVSYDNNDS 1066
Cdd:TIGR03786    1 FSPLTFTKVGTYTYTVTEVK--GKEPGVTYDTTVHTVTVTVTDDEQGKLVATVIYDKEKN 58
ClfA COG4932
Clumping factor A-related surface protein, MSCRAMM (microbial surface components recognizing ...
 352-695 1.15e-09

Clumping factor A-related surface protein, MSCRAMM (microbial surface components recognizing adhesive matrix molecules) family, DEv-IgG fold [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 443959 [Multi-domain]  Cd Length: 689  Bit Score: 62.68  E-value: 1.15e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  352 KTVKSVGASVTADIAGKIKITKYDEADATVKLAGVVFEIrEKTTNNLVDTVTTDADGIAVSKALNNGKYIVKEKTPKSGY 431
Cdd:COG4932   343 TTTVTVTNGNNEVKTGSVTLTKVDADDGEAPLAGAEFTL-TDADGTVVATITTDADGTASFKGLAPGTYTLTETKAPEGY 421

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  432 QVNSQEFEVEMKDG-KGVPLSISNKRVTVDFEATKTWVNGKATDYKEVKLGLYVHKEGQTVADAKPVSGNYTPEVTKSNG 510
Cdd:COG4932   422 TLDSTPITVTVTDGgTGAIDTITNERKKGSVQVTKVDAPLAGATFTLTDADGTVVTLTTDADLAGATFEADGKVVTTTDA 501

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  511 VYTYKWKNQLPKYDVDGTTELIYSVRELQDQTNIPLDETETVVGGTSDYVVSYNEDKTKITNTYKPAKISVTAKKVWVGG 590
Cdd:COG4932   502 SGKYTFKNLPPGTYTDAGGSATVITDDTDGTVGDEATGTDPEVTVTGKSTTTTPDVALLTNLGTTEDALTSLAKTGDEVG 581

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  591 QEHIRPTVYFKLYRTPEGGAIEEVAGAEQKEVPKTNGTLEWPDLPATDEHGVKYTYSVMEVDEDGNLIVDTIDGYTPEQT 670
Cdd:COG4932   582 KGLTLTTTTTVDTLDTNATEKTETVTVTAQLIGVKTTKLTDTTDPKGGTVEEATTTGGTANTGKTGTDLTDDTTVTSTTN 661

                         330       340
                  ....*....|....*....|....*
gi 446281531  671 ADLTVTNTYSTSPTKADIEVKKELT 695
Cdd:COG4932   662 TATSVEDTTDVDNKDAFTGGTEPGG 686
SpaA pfam17802
Prealbumin-like fold domain; This entry contains a prealbumin-like domain from a wide variety ...
 378-446 1.59e-09

Prealbumin-like fold domain; This entry contains a prealbumin-like domain from a wide variety of bacterial surface proteins. This entry corresponds to domain 1 and domain 3 of SpaA from Corynebacterium diphtheriae. Some members of this family contain an isopeptide bond.


Pssm-ID: 465513 [Multi-domain]  Cd Length: 72  Bit Score: 55.28  E-value: 1.59e-09
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446281531   378 DATVKLAGVVFEIR---EKTTNNLVDTVTTDADGIAVSKALNNGKYIVKEKTPKSGYQVNSQEFEVEMKDGK 446
Cdd:pfam17802    1 DTGKPLAGAEFTLYdadGTVDGKVVGTLTTDEDGKATFDGLPPGTYTLKETKAPDGYVLDDTPIEFTVTEDG 72
Big_8 pfam17961
Bacterial Ig domain; This entry represents a bacterial Ig-fold domain that is found in a wide ...
 65-144 3.08e-08

Bacterial Ig domain; This entry represents a bacterial Ig-fold domain that is found in a wide range of bacterial cell surface adherence proteins.


Pssm-ID: 465589 [Multi-domain]  Cd Length: 102  Bit Score: 52.61  E-value: 3.08e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531    65 KLKYSGKFSFPNvaanEIKDGDYFIVKAPDNLSL-----TDGSLDLIDSTSNIkMGTVRVDNANHQLVFTFNDKVKDKQN 139
Cdd:pfam17961    7 SLKLKADFSLGD----SVKEGDYFTIKLPDNLKFygintSDKSFDIKDDNGEV-IAKGTYDPGTGTITYTFTDYVENKSN 81


                   ....*
gi 446281531   140 IRGDF 144
Cdd:pfam17961   82 IKGSL 86
strep_pil_rpt TIGR03786
streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once ...
 732-786 1.80e-07

streptococcal pilin isopeptide linkage domain; This model describes a domain that occurs once in the major pilin of Streptococcus pyogenes, Spy0128, but in higher copy numbers in other streptococcal proteins. The domain occurs nine times in a surface-anchored protein of Bifidobacterium longum. All members of this family have LPXTG-type sortase target sequences. The S. pyogenes major pilin has been shown to undergo isopeptide bond cross-linking, mediated by sortases, that are critical to maintaining pilus structural integrity. One such Lys-to-Asn isopeptide bond is to a near-invariant Asn near the C-terminal end of this domain (column 81 of the seed alignment). A Glu in the S. pyogenes major pilin (column 25 of the seed alignment), invariant as Glu or Gln, is described as catalytic for isopeptide bond formation.


Pssm-ID: 274781 [Multi-domain]  Cd Length: 63  Bit Score: 49.22  E-value: 1.80e-07
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*
gi 446281531   732 FKDIPFDKAGEYEFTVVEVNagQTIDGVTHDSRKVPVTVSVTDDGKGKLVATVLY 786
Cdd:TIGR03786    1 FSPLTFTKVGTYTYTVTEVK--GKEPGVTYDTTVHTVTVTVTDDEQGKLVATVIY 53
CollagenBindB cd00222
Repeat unit of collagen-binding protein domain B; The collagen-binding protein mediates ...
 579-679 1.91e-06

Repeat unit of collagen-binding protein domain B; The collagen-binding protein mediates bacterial adherence to collagen; the primary sequence has a non-repetitive, collagen-binding A region, followed by instances of this B region repetitive unit. The B region has one to four 23 kDa repeat units (B1-B4), which have been suggested to serve as 'stalks' that project the A region from the bacterial surface and thus facilitate bacterial adherence to collagen. Each B repeat unit has two highly similar domains (D1 and D2) placed side-by-side; both D1 and D2 are included in this model. They exhibit a unique inverse IgG-like domain fold.


Pssm-ID: 212461 [Multi-domain]  Cd Length: 92  Bit Score: 47.29  E-value: 1.91e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  579 ISVTAKKVWVGG--QEHIRPT-VYFKLYRTPEggaieeVAGAEQKEVPKTNGTLEWPDLPATDEHGVKYTYSVMEVDedg 655
Cdd:cd00222     1 TDITVTKTWDDDdnQDGKRPEsITVQLLANGK------ETGDTVTLTASNDWTYTFTNLPKYDENGKEITYTVKEVP--- 71

                          90       100
                  ....*....|....*....|....*..
gi 446281531  656 nlivdtIDGYTPEQTAD---LTVTNTY 679
Cdd:cd00222    72 ------VPGYTTTVTGDdggFTITNTH 92
Cna_B pfam05738
Cna protein B-type domain; This domain is found in Staphylococcus aureus collagen-binding ...
 581-678 3.34e-06

Cna protein B-type domain; This domain is found in Staphylococcus aureus collagen-binding surface protein. The structure of the repetitive B-region has been solved and forms a beta sandwich structure.


Pssm-ID: 461726 [Multi-domain]  Cd Length: 88  Bit Score: 46.47  E-value: 3.34e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531   581 VTAKKVWV--GGQEHIRPT-VYFKLYRtpEGGAIeevagAEQKEVPKTNG-TLEWPDLPATDEHGVKYTYSVMEVDEDG- 655
Cdd:pfam05738    1 VSVTKVWDdnNNQDGIRPEsITVQLLA--NGQKV-----GVTKELTAANNwTYTFTDLPKYDENGKEITYTVEEDAVPGy 73

                           90       100
                   ....*....|....*....|...
gi 446281531   656 NLIVDTIDGYtpeqtadlTVTNT 678
Cdd:pfam05738   74 TTTVDAKDGF--------TITNT 88
ClfA COG4932
Clumping factor A-related surface protein, MSCRAMM (microbial surface components recognizing ...
 545-1291 1.07e-05

Clumping factor A-related surface protein, MSCRAMM (microbial surface components recognizing adhesive matrix molecules) family, DEv-IgG fold [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 443959 [Multi-domain]  Cd Length: 689  Bit Score: 49.97  E-value: 1.07e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  545 PLDETETVVGGTSDYVVSYNEDKTKITNTYKPAKISVTAKKVWVGGQEHIRPTVYFKLYRTPEGGAIEEVAGAEQKEVPK 624
Cdd:COG4932    23 SLVAVTTGAVLGLALGSTGGTAGSAAAINSAPATGTATGTSAGATAVIVIAAGLTATPTETASGLGGDATVTTTANVAKV 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  625 TNGTLEWPDLPATDEHGVKYTYSVMEVDEDGNLIVDTIDGYTPEQTADLTVTNTYSTSPTKADIEVKKELTGGRPTPLQN 704
Cdd:COG4932   103 TNGAAANLTVNADGTASNAGTLAKGAETATGNLDGDAGDVTVTAAATDGVNDVDGNGASVTDSVTLKKVDDGDTGKPLPG 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  705 EEFEfiLKDKNGQEVQRAKNDAAGRVVFKDIPfdkAGEYEFTVVEVNAGQTIDGVTHDSRKVPVTVsvtddgkgklvatv 784
Cdd:COG4932   183 ATFT--LYDSDGTLVKTVTTDADGKYTFTDLP---PGTYTLTETKAPEGYVLDTKDPTGATITVTV-------------- 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  785 lyhpitavalpsanlassapgsnlvppltgavnraTDAGIQTFKNTYKATNAKAVLEVTKQLTgrTTGAQENEFEFTLTD 864
Cdd:COG4932   244 -----------------------------------NAGGTVTVTLKNTPKYTKGSVTVTKTDA--DTGEPLAGATFTLTD 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  865 QVGNVEKAKN----GADGKVKFSELtfdEAGTYTYTIKEVKAGttengitYDSKTVTAKVTVTDdgqgklhatveyssdG 940
Cdd:COG4932   287 ADGNTVVTTTvtvtDADGSYTFTDL---PPGTYTVTETKAPAG-------YDLDGEAVKVTITA---------------G 341

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531  941 TANSTTFTNTYNPAKvKAPVSATKsfiNKNTNTPMPLKGGEFvfSLINHEGDPVEKTTNDADGNIKFEDLEFNKadtyhY 1020
Cdd:COG4932   342 QTTTVTVTNGNNEVK-TGSVTLTK---VDADDGEAPLAGAEF--TLTDADGTVVATITTDADGTASFKGLAPGT-----Y 410

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531 1021 TIVEKNAGTtdkGITYSNKTIEVTIKvvdnGNGALEATVSYDNNDSTFENTYKADNAKAGLEVMKTLSGRELKADEFDFV 1100
Cdd:COG4932   411 TLTETKAPE---GYTLDSTPITVTVT----DGGTGAIDTITNERKKGSVQVTKVDAPLAGATFTLTDADGTVVTLTTDAD 483

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531 1101 LKNDADGSELQKVKNT-ADGKVTF---APIEYTKAGTYNYTIVETNAGKTVNGVTYDNLEVKVTVEVTDDSEGKLHANVT 1176
Cdd:COG4932   484 LAGATFEADGKVVTTTdASGKYTFknlPPGTYTDAGGSATVITDDTDGTVGDEATGTDPEVTVTGKSTTTTPDVALLTNL 563

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446281531 1177 YSADKEFNNSYGASKAKAALAVKKTLTGRELKADEFEFTLTDQAGTLKETVKNDKDGNVKFSALEFDKAGTFTYKIAEKA 1256
Cdd:COG4932   564 GTTEDALTSLAKTGDEVGKGLTLTTTTTVDTLDTNATEKTETVTVTAQLIGVKTTKLTDTTDPKGGTVEEATTTGGTANT 643

                         730       740       750
                  ....*....|....*....|....*....|....*
gi 446281531 1257 GTATGIQYDTKTITATVTVADNGKGVLEATVAYDD 1291
Cdd:COG4932   644 GKTGTDLTDDTTVTSTTNTATSVEDTTDVDNKDAF 678
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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