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Conserved domains on  [gi|446353878|ref|WP_000431733|]
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MULTISPECIES: GNAT family N-acetyltransferase [Bacillus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 707069)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate; similar to Bacillus subtilis protein YyaT

CATH:  3.40.630.30
Gene Ontology:  GO:0008080
SCOP:  4001800

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ElaA super family cl28579
Predicted N-acyltransferase, GNAT family [General function prediction only];
48-133 1.33e-12

Predicted N-acyltransferase, GNAT family [General function prediction only];


The actual alignment was detected with superfamily member COG2153:

Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 60.20  E-value: 1.33e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878  48 IYVFDDKRLVGMGRVISDGVITGIICGVGVLPKYQSSGIGKEIVERLIQHCEQNKVIP-QLMCVEKLQSYYESIGFEAFS 126
Cdd:COG2153   37 LLAYDDGELVATARLLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRiVLSAQAHAVGFYEKLGFVPVG 116

                         90
                 ....*....|....*..
gi 446353878 127 ----------IGMTKHI 133
Cdd:COG2153  117 eefleagiphIDMRKPL 133
 
Name Accession Description Interval E-value
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
48-133 1.33e-12

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 60.20  E-value: 1.33e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878  48 IYVFDDKRLVGMGRVISDGVITGIICGVGVLPKYQSSGIGKEIVERLIQHCEQNKVIP-QLMCVEKLQSYYESIGFEAFS 126
Cdd:COG2153   37 LLAYDDGELVATARLLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRiVLSAQAHAVGFYEKLGFVPVG 116

                         90
                 ....*....|....*..
gi 446353878 127 ----------IGMTKHI 133
Cdd:COG2153  117 eefleagiphIDMRKPL 133
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 36-126 3.20e-12

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 59.21  E-value: 3.20e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878   36 ELERMCKQSWYAIYV-FDDKRLVGMGRVISDGVITGIIcgvgVLPKYQSSGIGKEIVERLIQHCEQNKVIPQLMCVE--- 111
Cdd:pfam13673  21 ALRERIDQGEYFFFVaFEGGQIVGVIALRDRGHISLLF----VDPDYQGQGIGKALLEAVEDYAEKDGIKLSELTVNasp 96

                          90
                  ....*....|....*
gi 446353878  112 KLQSYYESIGFEAFS 126
Cdd:pfam13673  97 YAVPFYEKLGFRATG 111
PLN02706 PLN02706
glucosamine 6-phosphate N-acetyltransferase
 48-132 1.74e-07

glucosamine 6-phosphate N-acetyltransferase


Pssm-ID: 178308 [Multi-domain]  Cd Length: 150  Bit Score: 47.01  E-value: 1.74e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878  48 IYVFDDKRLvgmGRVISDGVI------------TGIICGVGVLPKYQSSGIGKEIVERLIQHCEQN---KVIpqLMCVEK 112
Cdd:PLN02706  55 ICVIEDAAS---GRIIATGSVfverkfirncgkVGHIEDVVVDSAARGKGLGKKIIEALTEHARSAgcyKVI--LDCSEE 129

                         90       100
                 ....*....|....*....|
gi 446353878 113 LQSYYESIGFEAFSIGMTKH 132
Cdd:PLN02706 130 NKAFYEKCGYVRKEIQMVKY 149
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 50-103 6.15e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 41.11  E-value: 6.15e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 446353878  50 VFDDKRLVGMGRVISDGVI--TGIICGVGVLPKYQSSGIGKEIVERLIQHCEQNKV 103
Cdd:cd04301    4 AEDDGEIVGFASLSPDGSGgdTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGA 59
 
Name Accession Description Interval E-value
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
48-133 1.33e-12

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 60.20  E-value: 1.33e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878  48 IYVFDDKRLVGMGRVISDGVITGIICGVGVLPKYQSSGIGKEIVERLIQHCEQNKVIP-QLMCVEKLQSYYESIGFEAFS 126
Cdd:COG2153   37 LLAYDDGELVATARLLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRiVLSAQAHAVGFYEKLGFVPVG 116

                         90
                 ....*....|....*..
gi 446353878 127 ----------IGMTKHI 133
Cdd:COG2153  117 eefleagiphIDMRKPL 133
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 36-126 3.20e-12

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 59.21  E-value: 3.20e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878   36 ELERMCKQSWYAIYV-FDDKRLVGMGRVISDGVITGIIcgvgVLPKYQSSGIGKEIVERLIQHCEQNKVIPQLMCVE--- 111
Cdd:pfam13673  21 ALRERIDQGEYFFFVaFEGGQIVGVIALRDRGHISLLF----VDPDYQGQGIGKALLEAVEDYAEKDGIKLSELTVNasp 96

                          90
                  ....*....|....*
gi 446353878  112 KLQSYYESIGFEAFS 126
Cdd:pfam13673  97 YAVPFYEKLGFRATG 111
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
52-124 3.01e-09

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 51.62  E-value: 3.01e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446353878  52 DDKRLVGMGRV----ISDGVITGIICGVGVLPKYQSSGIGKEIVERLIQHCEQN--KVIpQLMCVEKLQSYYESIGFEA 124
Cdd:COG3153   46 DDGEIVGHVALspvdIDGEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERgaRAV-VLLGDPSLLPFYERFGFRP 123
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 36-122 1.42e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 49.44  E-value: 1.42e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878   36 ELERMCKQSWYAIYVFDDKRLVGM--GRVISDGVITGIICGVGVLPKYQSSGIGKEIVERLIQHCEQNKVIPQLMCVEK- 112
Cdd:pfam00583  24 LEDWDEDASEGFFVAEEDGELVGFasLSIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAAd 103

                          90
                  ....*....|...
gi 446353878  113 ---LQSYYESIGF 122
Cdd:pfam00583 104 nlaAIALYEKLGF 116
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 44-123 1.65e-08

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 48.60  E-value: 1.65e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878   44 SWYAIYV-FDDKRLVGMGRV-ISDGVITGIICGVGVLPKYQSSGIGKEIVERLIQHCEQNKVIP-QLMCVEKLQSYYESI 120
Cdd:pfam13508   1 PGGRFFVaEDDGKIVGFAALlPLDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLlELETTNRAAAFYEKL 80


                  ...
gi 446353878  121 GFE 123
Cdd:pfam13508  81 GFE 83
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 52-123 3.25e-08

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 48.83  E-value: 3.25e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446353878  52 DDKRLVGMGRVISDGVITGIICGVGVLPKYQSSGIGKEIVERLIQHCEQNKvIPQLMCV--EKLQSYYESIGFE 123
Cdd:COG1246   35 EDGEIVGCAALHPLDEDLAELRSLAVHPDYRGRGIGRRLLEALLAEARELG-LKRLFLLttSAAIHFYEKLGFE 107
PLN02706 PLN02706
glucosamine 6-phosphate N-acetyltransferase
 48-132 1.74e-07

glucosamine 6-phosphate N-acetyltransferase


Pssm-ID: 178308 [Multi-domain]  Cd Length: 150  Bit Score: 47.01  E-value: 1.74e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878  48 IYVFDDKRLvgmGRVISDGVI------------TGIICGVGVLPKYQSSGIGKEIVERLIQHCEQN---KVIpqLMCVEK 112
Cdd:PLN02706  55 ICVIEDAAS---GRIIATGSVfverkfirncgkVGHIEDVVVDSAARGKGLGKKIIEALTEHARSAgcyKVI--LDCSEE 129

                         90       100
                 ....*....|....*....|
gi 446353878 113 LQSYYESIGFEAFSIGMTKH 132
Cdd:PLN02706 130 NKAFYEKCGYVRKEIQMVKY 149
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
55-123 2.74e-07

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 45.28  E-value: 2.74e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446353878  55 RLVGMGRVISDGVITGIICGVGVLPKYQSSGIGKEIVERLIQHC-EQNKVIPQLMCV---EKLQSYYESIGFE 123
Cdd:COG3393    1 ELVAMAGVRAESPGVAEISGVYTHPEYRGRGLASALVAALAREAlARGARTPFLYVDadnPAARRLYERLGFR 73
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 11-126 4.56e-06

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 43.12  E-value: 4.56e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878  11 HPTDFNGLLsLYESLEwnslkltvNELERMCKQSWYAIYVF--DDKRLVGMG--RVISDGVitGIICGVGVLPKYQSSGI 86
Cdd:COG0454    7 TPEDINFIL-LIEALD--------AELKAMEGSLAGAEFIAvdDKGEPIGFAglRRLDDKV--LELKRLYVLPEYRGKGI 75

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 446353878  87 GKEIVERLIQHC-EQNKVIPQLMCV---EKLQSYYESIGFEAFS 126
Cdd:COG0454   76 GKALLEALLEWArERGCTALELDTLdgnPAAIRFYERLGFKEIE 119
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 50-103 6.15e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 41.11  E-value: 6.15e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 446353878  50 VFDDKRLVGMGRVISDGVI--TGIICGVGVLPKYQSSGIGKEIVERLIQHCEQNKV 103
Cdd:cd04301    4 AEDDGEIVGFASLSPDGSGgdTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGA 59
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 69-123 1.06e-05

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 41.18  E-value: 1.06e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878  69 TGIICGVGVLPKYQSSGIGKEIVERLIQHCEQNKvIPQLMCV-----EKLQSYYESIGFE 123
Cdd:COG0456   13 EAEIEDLAVDPEYRGRGIGRALLEAALERARERG-ARRLRLEvrednEAAIALYEKLGFE 71
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 36-103 2.42e-03

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 35.62  E-value: 2.42e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446353878   36 ELERMCKQSWYaIYVFDDKRLVGMGRVIS-----DGVI--TGIICGVGVLPKYQSSGIGKEIVERLIQHCEQNKV 103
Cdd:pfam13527  31 YFRPLLEEGRV-LGAFDDGELVSTLALYPfelnvPGKTlpAAGITGVATYPEYRGRGVMSRLLRRSLEEMRERGV 104
Eis COG4552
Predicted acetyltransferase [General function prediction only];
50-130 9.99e-03

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 34.87  E-value: 9.99e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446353878  50 VFDDKRLVGMGRVISDGVI-------TGIICGVGVLPKYQSSGIGKEIVERLIQHC-EQNKVIPQLMCVEklQSYYESIG 121
Cdd:COG4552   46 VFDDGELVGTLALYPFTLNvggarvpMAGITGVAVAPEHRRRGVARALLREALAELrERGQPLSALYPFE--PGFYRRFG 123


                 ....*....
gi 446353878 122 FEAFSIGMT 130
Cdd:COG4552  124 YELAGDRRR 132
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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