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Conserved domains on  [gi|446369059|ref|WP_000446914|]
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MULTISPECIES: Bax inhibitor-1/YccA family protein [Enterobacteriaceae]

Protein Classification

Bax inhibitor-1/YccA family protein( domain architecture ID 10179639)

Bax inhibitor-1/YccA family protein similar to Escherichia coli YccA, which negatively regulates the FtsH protease and is degraded by in an FtsH-dependent manner

Gene Ontology:  GO:0016020
PubMed:  18336295|18440869
TCDB:  1.A.14.2

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
BI-1-like_bacterial cd10432
Bacterial BAX inhibitor (BI)-1/YccA-like proteins; This family is comprised of bacterial ...
 15-223 7.80e-39

Bacterial BAX inhibitor (BI)-1/YccA-like proteins; This family is comprised of bacterial relatives of the mammalian members of the BAX inhibitor (BI)-1 like family of small transmembrane proteins, which have been shown to have an antiapoptotic effect either by stimulating the antiapoptotic function of Bcl-2, a well-characterized oncogene, or by inhibiting the proapoptotic effect of Bax, another member of the Bcl-2 family. In plants, BI-1 like proteins play a role in pathogen resistance. A characterized prokaryotic member, Escherichia coli YccA, has been shown to interact with ATP-dependent protease FtsH, which degrades abnormal membrane proteins as part of a quality control mechanism to keep the integrity of biological membranes.


:

Pssm-ID: 198414  Cd Length: 211  Bit Score: 133.84  E-value: 7.80e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059  15 HEARILNGIWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGV---IYLFLLS 91
Cdd:cd10432    1 GLRSFMKKVYGLMAAGLLLTAVGAYVGASTPALLLIAGSPLMWVLLIAELALVFGLSFRINKMSVATALPlffAFAALTG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059  92 LVAIVVASLVPVYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVL 171
Cdd:cd10432   81 LTLSPILLVYTGASIAQAFFTTAATFGGLSLYGYTTKKDLSFLGSFLFMGLIGLIIASLVNIFLQSSALQFAISAIGVLI 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 446369059 172 WPGIISHGRNKLLELAGKCHSeelWSPVRCAFTGALTLYYYFIGFFGILAAI 223
Cdd:cd10432  161 FSGLIAYDTQRIKRMYEAGGG---ESGYKDAIMGALSLYLDFINLFLFLLRL 209
 
Name Accession Description Interval E-value
BI-1-like_bacterial cd10432
Bacterial BAX inhibitor (BI)-1/YccA-like proteins; This family is comprised of bacterial ...
 15-223 7.80e-39

Bacterial BAX inhibitor (BI)-1/YccA-like proteins; This family is comprised of bacterial relatives of the mammalian members of the BAX inhibitor (BI)-1 like family of small transmembrane proteins, which have been shown to have an antiapoptotic effect either by stimulating the antiapoptotic function of Bcl-2, a well-characterized oncogene, or by inhibiting the proapoptotic effect of Bax, another member of the Bcl-2 family. In plants, BI-1 like proteins play a role in pathogen resistance. A characterized prokaryotic member, Escherichia coli YccA, has been shown to interact with ATP-dependent protease FtsH, which degrades abnormal membrane proteins as part of a quality control mechanism to keep the integrity of biological membranes.


Pssm-ID: 198414  Cd Length: 211  Bit Score: 133.84  E-value: 7.80e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059  15 HEARILNGIWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGV---IYLFLLS 91
Cdd:cd10432    1 GLRSFMKKVYGLMAAGLLLTAVGAYVGASTPALLLIAGSPLMWVLLIAELALVFGLSFRINKMSVATALPlffAFAALTG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059  92 LVAIVVASLVPVYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVL 171
Cdd:cd10432   81 LTLSPILLVYTGASIAQAFFTTAATFGGLSLYGYTTKKDLSFLGSFLFMGLIGLIIASLVNIFLQSSALQFAISAIGVLI 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 446369059 172 WPGIISHGRNKLLELAGKCHSeelWSPVRCAFTGALTLYYYFIGFFGILAAI 223
Cdd:cd10432  161 FSGLIAYDTQRIKRMYEAGGG---ESGYKDAIMGALSLYLDFINLFLFLLRL 209
Bax1-I pfam01027
Inhibitor of apoptosis-promoting Bax1; Programmed cell-death involves a set of Bcl-2 family ...
 18-223 2.57e-14

Inhibitor of apoptosis-promoting Bax1; Programmed cell-death involves a set of Bcl-2 family proteins, some of which inhibit apoptosis (Bcl-2 and Bcl-XL) and some of which promote it (Bax and Bak). Human Bax inhibitor, BI-1, is an evolutionarily conserved integral membrane protein containing multiple membrane-spanning segments predominantly localized to intracellular membranes. It has 6-7 membrane-spanning domains. The C termini of the mammalian BI-1 proteins are comprised of basic amino acids resembling some nuclear targeting sequences, but otherwise the predicted proteins lack motifs that suggest a function. As plant BI-1 appears to localize predominantly to the ER, we hypothesized that plant BI-1 could also regulate cell death triggered by ER stress. BI-1 appears to exert its effect through an interaction with calmodulin. The budding yeast member of this family has been found unexpectedly to encode a BH3 domain-containing protein (Ybh3p) that regulates the mitochondrial pathway of apoptosis in a phylogenetically conserved manner. Examination of the crystal structure of a bacterial member of this family shows that these proteins mediate a calcium leak across the membrane that is pH-dependent. Calcium homoeostasis balances passive calcium leak with active calcium uptake. The structure exists in a pore-closed and pore-open conformation, at pHs of 8 and 6 respectively, and the pore can be opened by intracrystalline transition; together these findings suggest that pH controls the conformational transition.


Pssm-ID: 460029  Cd Length: 207  Bit Score: 69.13  E-value: 2.57e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059   18 RILNGIWLITALGLVATAGLAWGAKYLEITATKYDSPPMY---VAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVA 94
Cdd:pfam01027   2 QFLRKVYGLLALGLLLTAAVAYLVLSSPALLFPSLHPPLFwvlIIAPLGLLFGALLLARKRKYSSNVALLLLLAFTLLMG 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059   95 IVVASLVPVYA---IIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVL 171
Cdd:pfam01027  82 LTLGPLLLVYTgaiIATAFLGTAAIFGGLSLYALTTKRDLSFLGGFLFAGLIGLIVASLVNIFLPSSALSLAISYLGVLL 161

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 446369059  172 WPGIISHGRNKLLELAGKCHSEelwspvrCAFTGALTLYYYFIGFFGILAAI 223
Cdd:pfam01027 162 FSGFILYDTQRIIKRYGEYGDY-------DAILAALSLYLDFINLFLSLLRI 206
YbhL COG0670
Integral membrane protein YbhL, putative Ca2+ regulator, Bax inhibitor (BI-1)/TMBIM family ...
 17-176 5.74e-06

Integral membrane protein YbhL, putative Ca2+ regulator, Bax inhibitor (BI-1)/TMBIM family [Inorganic ion transport and metabolism];


Pssm-ID: 440434  Cd Length: 231  Bit Score: 45.94  E-value: 5.74e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059  17 ARILNGIWLITALGLVATAGLAWGAKYLE-ITATKYDSPPM-YVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVA 94
Cdd:COG0670   20 RKVLRNTYGLLALGLLLTALVAYLGMALPgLAALLFGSPGLfWVLLIAPLGLVFLLSKLANRANSAAALALLFAYTALMG 99

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059  95 IVVASLVPVYA-------IIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCL 167
Cdd:COG0670  100 LTLSPILLVYTglsgpasIAQAFGGTAATFGGLSLYGYTTKRDLSFLGSFLFMGLIGLIIASLVNIFLQSPALSLAISVA 179


                 ....*....
gi 446369059 168 MIVLWPGII 176
Cdd:COG0670  180 GVLLFSGLT 188
 
Name Accession Description Interval E-value
BI-1-like_bacterial cd10432
Bacterial BAX inhibitor (BI)-1/YccA-like proteins; This family is comprised of bacterial ...
 15-223 7.80e-39

Bacterial BAX inhibitor (BI)-1/YccA-like proteins; This family is comprised of bacterial relatives of the mammalian members of the BAX inhibitor (BI)-1 like family of small transmembrane proteins, which have been shown to have an antiapoptotic effect either by stimulating the antiapoptotic function of Bcl-2, a well-characterized oncogene, or by inhibiting the proapoptotic effect of Bax, another member of the Bcl-2 family. In plants, BI-1 like proteins play a role in pathogen resistance. A characterized prokaryotic member, Escherichia coli YccA, has been shown to interact with ATP-dependent protease FtsH, which degrades abnormal membrane proteins as part of a quality control mechanism to keep the integrity of biological membranes.


Pssm-ID: 198414  Cd Length: 211  Bit Score: 133.84  E-value: 7.80e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059  15 HEARILNGIWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGV---IYLFLLS 91
Cdd:cd10432    1 GLRSFMKKVYGLMAAGLLLTAVGAYVGASTPALLLIAGSPLMWVLLIAELALVFGLSFRINKMSVATALPlffAFAALTG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059  92 LVAIVVASLVPVYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVL 171
Cdd:cd10432   81 LTLSPILLVYTGASIAQAFFTTAATFGGLSLYGYTTKKDLSFLGSFLFMGLIGLIIASLVNIFLQSSALQFAISAIGVLI 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 446369059 172 WPGIISHGRNKLLELAGKCHSeelWSPVRCAFTGALTLYYYFIGFFGILAAI 223
Cdd:cd10432  161 FSGLIAYDTQRIKRMYEAGGG---ESGYKDAIMGALSLYLDFINLFLFLLRL 209
Bax1-I pfam01027
Inhibitor of apoptosis-promoting Bax1; Programmed cell-death involves a set of Bcl-2 family ...
 18-223 2.57e-14

Inhibitor of apoptosis-promoting Bax1; Programmed cell-death involves a set of Bcl-2 family proteins, some of which inhibit apoptosis (Bcl-2 and Bcl-XL) and some of which promote it (Bax and Bak). Human Bax inhibitor, BI-1, is an evolutionarily conserved integral membrane protein containing multiple membrane-spanning segments predominantly localized to intracellular membranes. It has 6-7 membrane-spanning domains. The C termini of the mammalian BI-1 proteins are comprised of basic amino acids resembling some nuclear targeting sequences, but otherwise the predicted proteins lack motifs that suggest a function. As plant BI-1 appears to localize predominantly to the ER, we hypothesized that plant BI-1 could also regulate cell death triggered by ER stress. BI-1 appears to exert its effect through an interaction with calmodulin. The budding yeast member of this family has been found unexpectedly to encode a BH3 domain-containing protein (Ybh3p) that regulates the mitochondrial pathway of apoptosis in a phylogenetically conserved manner. Examination of the crystal structure of a bacterial member of this family shows that these proteins mediate a calcium leak across the membrane that is pH-dependent. Calcium homoeostasis balances passive calcium leak with active calcium uptake. The structure exists in a pore-closed and pore-open conformation, at pHs of 8 and 6 respectively, and the pore can be opened by intracrystalline transition; together these findings suggest that pH controls the conformational transition.


Pssm-ID: 460029  Cd Length: 207  Bit Score: 69.13  E-value: 2.57e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059   18 RILNGIWLITALGLVATAGLAWGAKYLEITATKYDSPPMY---VAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVA 94
Cdd:pfam01027   2 QFLRKVYGLLALGLLLTAAVAYLVLSSPALLFPSLHPPLFwvlIIAPLGLLFGALLLARKRKYSSNVALLLLLAFTLLMG 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059   95 IVVASLVPVYA---IIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVL 171
Cdd:pfam01027  82 LTLGPLLLVYTgaiIATAFLGTAAIFGGLSLYALTTKRDLSFLGGFLFAGLIGLIVASLVNIFLPSSALSLAISYLGVLL 161

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 446369059  172 WPGIISHGRNKLLELAGKCHSEelwspvrCAFTGALTLYYYFIGFFGILAAI 223
Cdd:pfam01027 162 FSGFILYDTQRIIKRYGEYGDY-------DAILAALSLYLDFINLFLSLLRI 206
YbhL COG0670
Integral membrane protein YbhL, putative Ca2+ regulator, Bax inhibitor (BI-1)/TMBIM family ...
 17-176 5.74e-06

Integral membrane protein YbhL, putative Ca2+ regulator, Bax inhibitor (BI-1)/TMBIM family [Inorganic ion transport and metabolism];


Pssm-ID: 440434  Cd Length: 231  Bit Score: 45.94  E-value: 5.74e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059  17 ARILNGIWLITALGLVATAGLAWGAKYLE-ITATKYDSPPM-YVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVA 94
Cdd:COG0670   20 RKVLRNTYGLLALGLLLTALVAYLGMALPgLAALLFGSPGLfWVLLIAPLGLVFLLSKLANRANSAAALALLFAYTALMG 99

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446369059  95 IVVASLVPVYA-------IIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCL 167
Cdd:COG0670  100 LTLSPILLVYTglsgpasIAQAFGGTAATFGGLSLYGYTTKRDLSFLGSFLFMGLIGLIIASLVNIFLQSPALSLAISVA 179


                 ....*....
gi 446369059 168 MIVLWPGII 176
Cdd:COG0670  180 GVLLFSGLT 188
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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