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Conserved domains on  [gi|446391054|ref|WP_000468909|]
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MULTISPECIES: M56 family metallopeptidase [Bacillus]

Protein Classification

M56 family metallopeptidase( domain architecture ID 1009010)

M56 family metallopeptidase that includes transmembrane proteins BlaR1 and MecR1, consisting of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Peptidase_M56 super family cl29776
BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which ...
 17-324 2.11e-61

BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which mediate staphylococcal resistance to beta-lactam antibiotics) is regulated by a signal-transducing integral membrane protein and a transcriptional repressor. The signal transducer is a fusion protein with penicillin-binding and zinc metalloprotease domains. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. homologs to this peptidase domain, which corresponds to Merops family M56, are also found in a number of other bacterial genome sequences.


The actual alignment was detected with superfamily member pfam05569:

Pssm-ID: 428523  Cd Length: 296  Bit Score: 199.23  E-value: 2.11e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054   17 VIETSILASVLVGFILCIKIVLKNWLTPRWKYALWLILIVRLILPWLPESSFSIY--SILSKGYESSQFFIQENTSIWHV 94
Cdd:pfam05569   3 LLRASIAAAAAVLVVLLLRRPLRRYLGAIWAYALWLIVPLALLAPFIPESALSNFttSAIFMARSKAPINSTAPKVIDDQ 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054   95 KENIHEPKTSISTQKITKevptggknnnwnlsvynILLLFWILGVLVSVFFMILINRRLYIY-VGKQPAITEKRVLDIFE 173
Cdd:pfam05569  83 ASPLATPFPVVAWSTILK-----------------ILLLLWIVGALILLLYMIAAYLVFRQRrVRRLGSLRAHELDDILK 145

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054  174 KCKKDMLIKKDISLSLSGKISSPTLFGIRNPRILLDENHVKYLNDNQLRYIFYHELSHFKRKDIIINLLMHCLLILNWFN 253
Cdd:pfam05569 146 EAKEDMGIKRPITISLSSNIDSPAVLGLWKPRIVLPADFDTRLSGEEIDYILAHELSHLKRGDLIINLLVAVLQCLHWFN 225

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446391054  254 PILWYAYYAMREDQEIACDNLALTFINPEEKIAYGQTIITLLEQYSTYYRTPTLANFSRNKAILKRRIIMI 324
Cdd:pfam05569 226 PLVHLAFRKIRIDQELACDAAVLARLHPHERKEYGRTLLKLLAGPSNHIVPVACVWLAGAKSALKERIMML 296
 
Name Accession Description Interval E-value
Peptidase_M56 pfam05569
BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which ...
 17-324 2.11e-61

BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which mediate staphylococcal resistance to beta-lactam antibiotics) is regulated by a signal-transducing integral membrane protein and a transcriptional repressor. The signal transducer is a fusion protein with penicillin-binding and zinc metalloprotease domains. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. homologs to this peptidase domain, which corresponds to Merops family M56, are also found in a number of other bacterial genome sequences.


Pssm-ID: 428523  Cd Length: 296  Bit Score: 199.23  E-value: 2.11e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054   17 VIETSILASVLVGFILCIKIVLKNWLTPRWKYALWLILIVRLILPWLPESSFSIY--SILSKGYESSQFFIQENTSIWHV 94
Cdd:pfam05569   3 LLRASIAAAAAVLVVLLLRRPLRRYLGAIWAYALWLIVPLALLAPFIPESALSNFttSAIFMARSKAPINSTAPKVIDDQ 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054   95 KENIHEPKTSISTQKITKevptggknnnwnlsvynILLLFWILGVLVSVFFMILINRRLYIY-VGKQPAITEKRVLDIFE 173
Cdd:pfam05569  83 ASPLATPFPVVAWSTILK-----------------ILLLLWIVGALILLLYMIAAYLVFRQRrVRRLGSLRAHELDDILK 145

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054  174 KCKKDMLIKKDISLSLSGKISSPTLFGIRNPRILLDENHVKYLNDNQLRYIFYHELSHFKRKDIIINLLMHCLLILNWFN 253
Cdd:pfam05569 146 EAKEDMGIKRPITISLSSNIDSPAVLGLWKPRIVLPADFDTRLSGEEIDYILAHELSHLKRGDLIINLLVAVLQCLHWFN 225

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446391054  254 PILWYAYYAMREDQEIACDNLALTFINPEEKIAYGQTIITLLEQYSTYYRTPTLANFSRNKAILKRRIIMI 324
Cdd:pfam05569 226 PLVHLAFRKIRIDQELACDAAVLARLHPHERKEYGRTLLKLLAGPSNHIVPVACVWLAGAKSALKERIMML 296
MecR1 COG4219
Signal transducer regulating beta-lactamase production, contains metallopeptidase domain ...
 135-378 2.31e-59

Signal transducer regulating beta-lactamase production, contains metallopeptidase domain [Signal transduction mechanisms];


Pssm-ID: 443363 [Multi-domain]  Cd Length: 337  Bit Score: 195.65  E-value: 2.31e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 135 WILGVLVSVFFMILINRRLYIYVGKQPAITEKRVLDIFEKCKKDMLIKKDISLSLSGKISSPTLFGIRNPRILLDENHVK 214
Cdd:COG4219    1 WLAGVLLLLLRLLISLLRLRRLLRRARPVTDEELLELLERLARRLGIRRPVRLLESDRITSPFSFGLLRPVILLPAGLEE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 215 YlNDNQLRYIFYHELSHFKRKDIIINLLMHCLLILNWFNPILWYAYYAMREDQEIACDNLALTFInpEEKIAYGQTIITL 294
Cdd:COG4219   81 L-SEEELEAILAHELAHIRRRDLLDNLLAELLLALFWFNPLVWLARRRLRLDRELACDAAVLKAG--GDRKAYAETLLKL 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 295 LEQYStyyRTPTLANFSRNKAILKRRIIMIKKFNKNSYRW--SAVGLATILGLSTLSLVNINAEETSYKKNIVEQPKKEV 372
Cdd:COG4219  158 AERRS---QPALALAFGGSKSTLKKRIKMLLKSKSKRRSRlkLLLALLLALLLALALLSAPEAAAEPEEAAAAAALEAAS 234


                 ....*.
gi 446391054 373 EQPKEI 378
Cdd:COG4219  235 AAASEA 240
M56_BlaR1_MecR1_like cd07341
Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; ...
 135-328 1.06e-48

Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain ?-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.


Pssm-ID: 320700 [Multi-domain]  Cd Length: 187  Bit Score: 162.88  E-value: 1.06e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 135 WILGVLVSVFFMIlinrRLYIYVGKQPAITEKRVLDIFEKCKKDMLIKKDISLSLSGKISSPTLFGIRNPRILLDENHVK 214
Cdd:cd07341    2 WLAGALLLLLRLL----RGLLRLRRLRRRAEPVPDSLLLELARRLGLRRSVRLSVSALVASPMVVGLFRPVILLPEGLLE 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 215 YlNDNQLRYIFYHELSHFKRKDIIINLLMHCLLILNWFNPILWYAYYAMREDQEIACDNLALTFINpeEKIAYGQTIITL 294
Cdd:cd07341   78 G-SPEELRAILLHELAHIRRRDLLVNLLQRLLEALFWFNPLVWLLSRRLRLERELACDEAVLAALG--DKEDYAEALLRL 154

                        170       180       190
                 ....*....|....*....|....*....|....
gi 446391054 295 LEQYSTYYRTPTLAnFSRNKAILKRRIIMIKKFN 328
Cdd:cd07341  155 AERRSQPPPALALA-LAGSKSLLKRRIKRILKKK 187
 
Name Accession Description Interval E-value
Peptidase_M56 pfam05569
BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which ...
 17-324 2.11e-61

BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which mediate staphylococcal resistance to beta-lactam antibiotics) is regulated by a signal-transducing integral membrane protein and a transcriptional repressor. The signal transducer is a fusion protein with penicillin-binding and zinc metalloprotease domains. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. homologs to this peptidase domain, which corresponds to Merops family M56, are also found in a number of other bacterial genome sequences.


Pssm-ID: 428523  Cd Length: 296  Bit Score: 199.23  E-value: 2.11e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054   17 VIETSILASVLVGFILCIKIVLKNWLTPRWKYALWLILIVRLILPWLPESSFSIY--SILSKGYESSQFFIQENTSIWHV 94
Cdd:pfam05569   3 LLRASIAAAAAVLVVLLLRRPLRRYLGAIWAYALWLIVPLALLAPFIPESALSNFttSAIFMARSKAPINSTAPKVIDDQ 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054   95 KENIHEPKTSISTQKITKevptggknnnwnlsvynILLLFWILGVLVSVFFMILINRRLYIY-VGKQPAITEKRVLDIFE 173
Cdd:pfam05569  83 ASPLATPFPVVAWSTILK-----------------ILLLLWIVGALILLLYMIAAYLVFRQRrVRRLGSLRAHELDDILK 145

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054  174 KCKKDMLIKKDISLSLSGKISSPTLFGIRNPRILLDENHVKYLNDNQLRYIFYHELSHFKRKDIIINLLMHCLLILNWFN 253
Cdd:pfam05569 146 EAKEDMGIKRPITISLSSNIDSPAVLGLWKPRIVLPADFDTRLSGEEIDYILAHELSHLKRGDLIINLLVAVLQCLHWFN 225

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446391054  254 PILWYAYYAMREDQEIACDNLALTFINPEEKIAYGQTIITLLEQYSTYYRTPTLANFSRNKAILKRRIIMI 324
Cdd:pfam05569 226 PLVHLAFRKIRIDQELACDAAVLARLHPHERKEYGRTLLKLLAGPSNHIVPVACVWLAGAKSALKERIMML 296
MecR1 COG4219
Signal transducer regulating beta-lactamase production, contains metallopeptidase domain ...
 135-378 2.31e-59

Signal transducer regulating beta-lactamase production, contains metallopeptidase domain [Signal transduction mechanisms];


Pssm-ID: 443363 [Multi-domain]  Cd Length: 337  Bit Score: 195.65  E-value: 2.31e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 135 WILGVLVSVFFMILINRRLYIYVGKQPAITEKRVLDIFEKCKKDMLIKKDISLSLSGKISSPTLFGIRNPRILLDENHVK 214
Cdd:COG4219    1 WLAGVLLLLLRLLISLLRLRRLLRRARPVTDEELLELLERLARRLGIRRPVRLLESDRITSPFSFGLLRPVILLPAGLEE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 215 YlNDNQLRYIFYHELSHFKRKDIIINLLMHCLLILNWFNPILWYAYYAMREDQEIACDNLALTFInpEEKIAYGQTIITL 294
Cdd:COG4219   81 L-SEEELEAILAHELAHIRRRDLLDNLLAELLLALFWFNPLVWLARRRLRLDRELACDAAVLKAG--GDRKAYAETLLKL 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 295 LEQYStyyRTPTLANFSRNKAILKRRIIMIKKFNKNSYRW--SAVGLATILGLSTLSLVNINAEETSYKKNIVEQPKKEV 372
Cdd:COG4219  158 AERRS---QPALALAFGGSKSTLKKRIKMLLKSKSKRRSRlkLLLALLLALLLALALLSAPEAAAEPEEAAAAAALEAAS 234


                 ....*.
gi 446391054 373 EQPKEI 378
Cdd:COG4219  235 AAASEA 240
M56_BlaR1_MecR1_like cd07341
Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; ...
 135-328 1.06e-48

Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain ?-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.


Pssm-ID: 320700 [Multi-domain]  Cd Length: 187  Bit Score: 162.88  E-value: 1.06e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 135 WILGVLVSVFFMIlinrRLYIYVGKQPAITEKRVLDIFEKCKKDMLIKKDISLSLSGKISSPTLFGIRNPRILLDENHVK 214
Cdd:cd07341    2 WLAGALLLLLRLL----RGLLRLRRLRRRAEPVPDSLLLELARRLGLRRSVRLSVSALVASPMVVGLFRPVILLPEGLLE 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 215 YlNDNQLRYIFYHELSHFKRKDIIINLLMHCLLILNWFNPILWYAYYAMREDQEIACDNLALTFINpeEKIAYGQTIITL 294
Cdd:cd07341   78 G-SPEELRAILLHELAHIRRRDLLVNLLQRLLEALFWFNPLVWLLSRRLRLERELACDEAVLAALG--DKEDYAEALLRL 154

                        170       180       190
                 ....*....|....*....|....*....|....
gi 446391054 295 LEQYSTYYRTPTLAnFSRNKAILKRRIIMIKKFN 328
Cdd:cd07341  155 AERRSQPPPALALA-LAGSKSLLKRRIKRILKKK 187
M56_BlaR1_MecR1_like cd07326
Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; ...
 199-321 4.91e-12

Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain ?-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.


Pssm-ID: 320685 [Multi-domain]  Cd Length: 165  Bit Score: 63.48  E-value: 4.91e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 199 FGIRNPRILLDENHVKYLNDNQLRYIFYHELSHFKRKDIIINLLMHCLLILNWFNPILWYAYYAMREDQEIACDNLALTF 278
Cdd:cd07326   42 LGGRRPRIVLSTGLLELLSPEELRAVLAHERAHLRRRDPLLLLLASALARALPFLPLLRRLAAAYRLLRELAADDAAARR 121

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 446391054 279 INPEekiAYGQTIITLLeQYSTYYRTPTLANFSrNKAILKRRI 321
Cdd:cd07326  122 VGPR---ALASALLKLA-RAGAPAAPAGALAFA-GAAVNEARI 159
M48_Ste24p_like cd07325
M48 Ste24 endopeptidase-like, integral membrane metallopeptidase; This family contains ...
 163-276 2.16e-03

M48 Ste24 endopeptidase-like, integral membrane metallopeptidase; This family contains peptidase M48 family Ste24p-like proteins that are as yet uncharacterized, but probably function as intracellular, membrane-associated zinc metalloproteases; they all contain the HEXXH Zn-binding motif, which is critical for Ste24p activity. They likely remove the C-terminal three residues of farnesylated proteins proteolytically and are possibly associated with the endoplasmic reticulum and golgi. Some members also contain ankyrin domains which occur in very diverse families of proteins and mediate protein-protein interactions.


Pssm-ID: 320684 [Multi-domain]  Cd Length: 199  Bit Score: 38.74  E-value: 2.16e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446391054 163 ITEK---RVLDIFEKCKKDMLIKK--DISLSLSGKISSPTLFGIRNPRILLDENHVKYLNDNQLRYIFYHELSHFKRKDI 237
Cdd:cd07325    7 VTPRqfpELHALLVEACRILGLKKvpELYVYQSPVLNAFALGFEGRPFIVLNSGLVELLDDDELRFVIGHELGHIKSGHV 86

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 446391054 238 IINLLMHCLLILNWFNPILW------YAYYAMREDQEIACDNLAL 276
Cdd:cd07325   87 LYRTLLLLLLLLGELIGILLlssalpLALLAWSRAAEYSADRAGL 131
HtpX COG0501
Zn-dependent protease with chaperone function [Posttranslational modification, protein ...
 199-270 3.60e-03

Zn-dependent protease with chaperone function [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440267 [Multi-domain]  Cd Length: 210  Bit Score: 38.33  E-value: 3.60e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446391054 199 FGIRNPRILLDENHVKYLNDNQLRYIFYHELSHFKRKDIIINLLMhcLLILNWFNPILWYAYYAMREDQEIA 270
Cdd:COG0501   37 RGPNNARIVVTDGLLELLDRDELEAVLAHELGHIKNGDILLMTLA--SGLLGLIGFLARLLPLAFGRDRDAG 106
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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