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Conserved domains on  [gi|446450799|ref|WP_000528654|]
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MULTISPECIES: GNAT family N-acetyltransferase [Bacillus]

Protein Classification

GNAT family protein( domain architecture ID 106742)

GNAT (Gcn5-related N-acetyltransferase) family protein similar to N-acetyltransferases that catalyze the transfer of an acetyl group from acetyl-CoA to a substrate

PubMed:  15581578

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NAT_SF super family cl17182
N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of ...
 18-130 2.46e-14

N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase which catalyze the transfer of an acetyl group to a substrate. The mechanism is an ordered Bi-Bi ternary complex kinetic mechanism for most GNATs: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and then CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/ph enylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


The actual alignment was detected with superfamily member pfam13527:

Pssm-ID: 473072 [Multi-domain]  Cd Length: 124  Bit Score: 67.98  E-value: 2.46e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446450799   18 EQLFPLFEEVFGIESQTLRDFtekgYWDYTYKA---LSFLQDNKVIANVAAFSLPLLMNGEKINAVGIQSVMTHPNHRRH 94
Cdd:pfam13527  10 DEVLRLLEYAFQDEDSPELRE----YFRPLLEEgrvLGAFDDGELVSTLALYPFELNVPGKTLPAAGITGVATYPEYRGR 85

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 446450799   95 GLMKQLFSKMIEEIDEQCEC-ALLFTENPELYKPFGF 130
Cdd:pfam13527  86 GVMSRLLRRSLEEMRERGVPlSFLYPSSYPIYRRFGY 122
 
Name Accession Description Interval E-value
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 18-130 2.46e-14

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 67.98  E-value: 2.46e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446450799   18 EQLFPLFEEVFGIESQTLRDFtekgYWDYTYKA---LSFLQDNKVIANVAAFSLPLLMNGEKINAVGIQSVMTHPNHRRH 94
Cdd:pfam13527  10 DEVLRLLEYAFQDEDSPELRE----YFRPLLEEgrvLGAFDDGELVSTLALYPFELNVPGKTLPAAGITGVATYPEYRGR 85

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 446450799   95 GLMKQLFSKMIEEIDEQCEC-ALLFTENPELYKPFGF 130
Cdd:pfam13527  86 GVMSRLLRRSLEEMRERGVPlSFLYPSSYPIYRRFGY 122
Eis COG4552
Predicted acetyltransferase [General function prediction only];
17-130 2.10e-09

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 57.60  E-value: 2.10e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446450799  17 REQLFPLFEEVFGIESqtlrDFTEKGYWDYTYKALSFL---QDNKVIANVAAFSLPLLMNGEKINAVGIQSVMTHPNHRR 93
Cdd:COG4552   11 LDAFARLLAYAFGPEP----DDEELEAYRPLLEPGRVLgvfDDGELVGTLALYPFTLNVGGARVPMAGITGVAVAPEHRR 86

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 446450799  94 HGLMKQLFSKMIEEI-DEQCECALLFTENPELYKPFGF 130
Cdd:COG4552   87 RGVARALLREALAELrERGQPLSALYPFEPGFYRRFGY 124
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 53-111 4.47e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 34.94  E-value: 4.47e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 446450799  53 FLQDNKVIANVAafslpLLMNGEKINAVGIQSVMTHPNHRRHGLMKQLFSKMIEEIDEQ 111
Cdd:cd04301    4 AEDDGEIVGFAS-----LSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARER 57
 
Name Accession Description Interval E-value
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 18-130 2.46e-14

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 67.98  E-value: 2.46e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446450799   18 EQLFPLFEEVFGIESQTLRDFtekgYWDYTYKA---LSFLQDNKVIANVAAFSLPLLMNGEKINAVGIQSVMTHPNHRRH 94
Cdd:pfam13527  10 DEVLRLLEYAFQDEDSPELRE----YFRPLLEEgrvLGAFDDGELVSTLALYPFELNVPGKTLPAAGITGVATYPEYRGR 85

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 446450799   95 GLMKQLFSKMIEEIDEQCEC-ALLFTENPELYKPFGF 130
Cdd:pfam13527  86 GVMSRLLRRSLEEMRERGVPlSFLYPSSYPIYRRFGY 122
Eis COG4552
Predicted acetyltransferase [General function prediction only];
17-130 2.10e-09

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 57.60  E-value: 2.10e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446450799  17 REQLFPLFEEVFGIESqtlrDFTEKGYWDYTYKALSFL---QDNKVIANVAAFSLPLLMNGEKINAVGIQSVMTHPNHRR 93
Cdd:COG4552   11 LDAFARLLAYAFGPEP----DDEELEAYRPLLEPGRVLgvfDDGELVGTLALYPFTLNVGGARVPMAGITGVAVAPEHRR 86

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 446450799  94 HGLMKQLFSKMIEEI-DEQCECALLFTENPELYKPFGF 130
Cdd:COG4552   87 RGVARALLREALAELrERGQPLSALYPFEPGFYRRFGY 124
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
78-138 3.58e-08

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 49.91  E-value: 3.58e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446450799  78 NAVGIQSVMTHPNHRRHGLMKQLFSKMIEEIDEQ-CECALLFT--ENPE---LYKPFGFQIVQEYLM 138
Cdd:COG3393   14 GVAEISGVYTHPEYRGRGLASALVAALAREALARgARTPFLYVdaDNPAarrLYERLGFRPVGEYAT 80
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
18-140 5.55e-08

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 50.85  E-value: 5.55e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446450799  18 EQLFPLFEEVFGI--ESQTLRDFTEKGYWDYTYKALsflQDNKVIANVAAFSLPLLMNGEkinAVGIQSVMTHPNHRRHG 95
Cdd:COG3153   10 EAIAALLRAAFGPgrEAELVDRLREDPAAGLSLVAE---DDGEIVGHVALSPVDIDGEGP---ALLLGPLAVDPEYRGQG 83

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 446450799  96 LMKQLFSKMIEEIDEQ-CECALLFT--ENPELYKPFGFQIVQEYLMEL 140
Cdd:COG3153   84 IGRALMRAALEAARERgARAVVLLGdpSLLPFYERFGFRPAGELGLTL 131
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 18-130 6.51e-05

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 41.73  E-value: 6.51e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446450799   18 EQLFPLFEEVFGIESQTLRDFTEKGYWDYT-YKALSFLQDNKVIAnvaafSLPLLMNGEKINAVGIQSVMTHPNHRRHGL 96
Cdd:pfam00583   2 EALYELLSEEFPEPWPDEPLDLLEDWDEDAsEGFFVAEEDGELVG-----FASLSIIDDEPPVGEIEGLAVAPEYRGKGI 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 446450799   97 MKQLFSKMIEE-IDEQCECALLFTENP-----ELYKPFGF 130
Cdd:pfam00583  77 GTALLQALLEWaRERGCERIFLEVAADnlaaiALYEKLGF 116
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 82-135 6.31e-04

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 39.20  E-value: 6.31e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 446450799  82 IQSVMTHPNHRRHGLMKQLFSKMIEEIDEQ-CECALLFT--ENPELYKPFGFQIVQE 135
Cdd:COG1246   55 LRSLAVHPDYRGRGIGRRLLEALLAEARELgLKRLFLLTtsAAIHFYEKLGFEEIDK 111
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 55-132 8.31e-04

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 37.82  E-value: 8.31e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446450799   55 QDNKVIANVAafslplLMNGEKINAVGIQSVMTHPNHRRHGLMKQLFsKMIEEIDEQCECALLFTENPE----LYKPFGF 130
Cdd:pfam13508  10 DDGKIVGFAA------LLPLDDEGALAELRLAVHPEYRGQGIGRALL-EAAEAAAKEGGIKLLELETTNraaaFYEKLGF 82


                  ..
gi 446450799  131 QI 132
Cdd:pfam13508  83 EE 84
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 82-135 2.06e-03

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 36.56  E-value: 2.06e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446450799  82 IQSVMTHPNHRRHGLMKQLFSKMIEEIDEQ-CECALLFT--ENP---ELYKPFGFQIVQE 135
Cdd:COG0456   16 IEDLAVDPEYRGRGIGRALLEAALERARERgARRLRLEVreDNEaaiALYEKLGFEEVGE 75
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 53-111 4.47e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 34.94  E-value: 4.47e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 446450799  53 FLQDNKVIANVAafslpLLMNGEKINAVGIQSVMTHPNHRRHGLMKQLFSKMIEEIDEQ 111
Cdd:cd04301    4 AEDDGEIVGFAS-----LSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARER 57
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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