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Conserved domains on  [gi|446454524|ref|WP_000532379|]
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MULTISPECIES: winged helix-turn-helix domain-containing protein [Escherichia]

Protein Classification

winged helix-turn-helix domain-containing protein( domain architecture ID 10007860)

winged helix-turn-helix (wHTH) domain-containing protein binds DNA and may function as a transcriptional regulator

CATH:  1.10.10.10
Gene Ontology:  GO:0003677
PubMed:  10473770|8831795|15808743
SCOP:  3000034

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CadC1 COG3710
DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];
3-106 7.58e-29

DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];


:

Pssm-ID: 442924 [Multi-domain]  Cd Length: 106  Bit Score: 105.76  E-value: 7.58e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446454524   3 RFLIGEtIVFDPDRRIIYSENQTVRLHSSCVYCLELLIEHQGEIVEHEAFYEYVWRrfGMEVGPNALYQSISMLRKALST 82
Cdd:COG3710    2 RYRFGD-FRLDPARRELLRDGEPVRLSPRAFDLLLLLLEHPGRVVSRDELLAAVWP--GRVVSDNSLTQAISRLRKALGD 78

                         90       100
                 ....*....|....*....|....
gi 446454524  83 CGTPDNIILTAPRRGFSLSQEICI 106
Cdd:COG3710   79 DGLEPRLIRTVPRRGYRLVAPVEV 102
 
Name Accession Description Interval E-value
CadC1 COG3710
DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];
3-106 7.58e-29

DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];


Pssm-ID: 442924 [Multi-domain]  Cd Length: 106  Bit Score: 105.76  E-value: 7.58e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446454524   3 RFLIGEtIVFDPDRRIIYSENQTVRLHSSCVYCLELLIEHQGEIVEHEAFYEYVWRrfGMEVGPNALYQSISMLRKALST 82
Cdd:COG3710    2 RYRFGD-FRLDPARRELLRDGEPVRLSPRAFDLLLLLLEHPGRVVSRDELLAAVWP--GRVVSDNSLTQAISRLRKALGD 78

                         90       100
                 ....*....|....*....|....
gi 446454524  83 CGTPDNIILTAPRRGFSLSQEICI 106
Cdd:COG3710   79 DGLEPRLIRTVPRRGYRLVAPVEV 102
trans_reg_C cd00383
DNA-binding effector domain of two-component system response regulators; Bacteria and some ...
 10-100 7.46e-11

DNA-binding effector domain of two-component system response regulators; Bacteria and some eukaryotes use two-component signal transduction systems to detect and respond to changes in the environment. The systems consists of a sensor histidine kinase and a response regulator. The former autophosphorylates a histidine residue on detecting an external stimulus. The phosphate is then transferred to an invariant aspartate residue in a highly conserved receiver domain of the response regulator. Phosphorylation activates a variable effector domain of the response regulator, which triggers the cellular response. This C-terminal effector domain belongs to the winged helix-turn-helix family of transcriptional regulators and contains DNA and RNA polymerase binding sites. Several dimers or monomers bind head to tail to small tandem repeats upstream of the genes. The RNA polymerase binding sites interact with the alpha or sigma subunit of RNA polymerase.


Pssm-ID: 294013 [Multi-domain]  Cd Length: 89  Bit Score: 57.49  E-value: 7.46e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446454524  10 IVFDPDRRIIYSENQTVRLHSSCVYCLELLIEHQGEIVEHEAFYEYVWRRfGMEVGPNALYQSISMLRKALSTCGTPDnI 89
Cdd:cd00383    1 LRLDPARREVTVDGEEVELTPKEFELLELLARNPGRVVSREELLEAVWGY-DYEVDDRTVDVHISRLRKKLEDDPEPR-L 78

                         90
                 ....*....|.
gi 446454524  90 ILTAPRRGFSL 100
Cdd:cd00383   79 IKTVRGVGYRL 89
Trans_reg_C smart00862
Transcriptional regulatory protein, C terminal; This domain is almost always found associated ...
 23-100 2.50e-09

Transcriptional regulatory protein, C terminal; This domain is almost always found associated with the response regulator receiver domain. It may play a role in DNA binding.


Pssm-ID: 214866 [Multi-domain]  Cd Length: 76  Bit Score: 52.94  E-value: 2.50e-09
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446454524    23 NQTVRLHSSCVYCLELLIEHQGEIVEHEAFYEYVWRRfGMEVGPNALYQSISMLRKALSTCGTPDnIILTAPRRGFSL 100
Cdd:smart00862   1 GEPIKLTPKEFRLLELLLRNPGRVVSREELLEAVWGD-DEDVDDNTLDVHISRLRKKLEDDGDPR-LIETVRGVGYRL 76
Trans_reg_C pfam00486
Transcriptional regulatory protein, C terminal;
35-100 3.23e-07

Transcriptional regulatory protein, C terminal;


Pssm-ID: 425712 [Multi-domain]  Cd Length: 77  Bit Score: 46.74  E-value: 3.23e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446454524   35 CLELLIEHQGEIVEHEAFYEYVWRRfGMEVGPNALYQSISMLRKALSTCGTPDNIILTAPRRGFSL 100
Cdd:pfam00486  13 LLELLAENPGRVVSREQLLEEVWGE-DYDVDDNTVDVHISRLRKKLEDDPRDPRLIKTVRGVGYRL 77
PRK10153 PRK10153
DNA-binding transcriptional activator CadC; Provisional
 4-100 7.64e-06

DNA-binding transcriptional activator CadC; Provisional


Pssm-ID: 236658 [Multi-domain]  Cd Length: 517  Bit Score: 46.96  E-value: 7.64e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446454524   4 FLIGETIVfDPDRRIIYSENQTVRLHSSCVYCLELLIEHQGEIVEHEAFYEYVWRRfgMEVGPNALYQSISMLRKALSTc 83
Cdd:PRK10153   6 FRIGEWLV-TPSENKISRQGREVTLEPRLIDLLVFFAQHSGEVLSRDELIDHVWDR--AIVTDHVVTQSISELRKSLKD- 81

                         90
                 ....*....|....*....
gi 446454524  84 GTPDNI--ILTAPRRGFSL 100
Cdd:PRK10153  82 GREDNPeyIVTVPKRGYKL 100
 
Name Accession Description Interval E-value
CadC1 COG3710
DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];
3-106 7.58e-29

DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];


Pssm-ID: 442924 [Multi-domain]  Cd Length: 106  Bit Score: 105.76  E-value: 7.58e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446454524   3 RFLIGEtIVFDPDRRIIYSENQTVRLHSSCVYCLELLIEHQGEIVEHEAFYEYVWRrfGMEVGPNALYQSISMLRKALST 82
Cdd:COG3710    2 RYRFGD-FRLDPARRELLRDGEPVRLSPRAFDLLLLLLEHPGRVVSRDELLAAVWP--GRVVSDNSLTQAISRLRKALGD 78

                         90       100
                 ....*....|....*....|....
gi 446454524  83 CGTPDNIILTAPRRGFSLSQEICI 106
Cdd:COG3710   79 DGLEPRLIRTVPRRGYRLVAPVEV 102
trans_reg_C cd00383
DNA-binding effector domain of two-component system response regulators; Bacteria and some ...
 10-100 7.46e-11

DNA-binding effector domain of two-component system response regulators; Bacteria and some eukaryotes use two-component signal transduction systems to detect and respond to changes in the environment. The systems consists of a sensor histidine kinase and a response regulator. The former autophosphorylates a histidine residue on detecting an external stimulus. The phosphate is then transferred to an invariant aspartate residue in a highly conserved receiver domain of the response regulator. Phosphorylation activates a variable effector domain of the response regulator, which triggers the cellular response. This C-terminal effector domain belongs to the winged helix-turn-helix family of transcriptional regulators and contains DNA and RNA polymerase binding sites. Several dimers or monomers bind head to tail to small tandem repeats upstream of the genes. The RNA polymerase binding sites interact with the alpha or sigma subunit of RNA polymerase.


Pssm-ID: 294013 [Multi-domain]  Cd Length: 89  Bit Score: 57.49  E-value: 7.46e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446454524  10 IVFDPDRRIIYSENQTVRLHSSCVYCLELLIEHQGEIVEHEAFYEYVWRRfGMEVGPNALYQSISMLRKALSTCGTPDnI 89
Cdd:cd00383    1 LRLDPARREVTVDGEEVELTPKEFELLELLARNPGRVVSREELLEAVWGY-DYEVDDRTVDVHISRLRKKLEDDPEPR-L 78

                         90
                 ....*....|.
gi 446454524  90 ILTAPRRGFSL 100
Cdd:cd00383   79 IKTVRGVGYRL 89
Trans_reg_C smart00862
Transcriptional regulatory protein, C terminal; This domain is almost always found associated ...
 23-100 2.50e-09

Transcriptional regulatory protein, C terminal; This domain is almost always found associated with the response regulator receiver domain. It may play a role in DNA binding.


Pssm-ID: 214866 [Multi-domain]  Cd Length: 76  Bit Score: 52.94  E-value: 2.50e-09
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446454524    23 NQTVRLHSSCVYCLELLIEHQGEIVEHEAFYEYVWRRfGMEVGPNALYQSISMLRKALSTCGTPDnIILTAPRRGFSL 100
Cdd:smart00862   1 GEPIKLTPKEFRLLELLLRNPGRVVSREELLEAVWGD-DEDVDDNTLDVHISRLRKKLEDDGDPR-LIETVRGVGYRL 76
Trans_reg_C pfam00486
Transcriptional regulatory protein, C terminal;
35-100 3.23e-07

Transcriptional regulatory protein, C terminal;


Pssm-ID: 425712 [Multi-domain]  Cd Length: 77  Bit Score: 46.74  E-value: 3.23e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446454524   35 CLELLIEHQGEIVEHEAFYEYVWRRfGMEVGPNALYQSISMLRKALSTCGTPDNIILTAPRRGFSL 100
Cdd:pfam00486  13 LLELLAENPGRVVSREQLLEEVWGE-DYDVDDNTVDVHISRLRKKLEDDPRDPRLIKTVRGVGYRL 77
PRK10153 PRK10153
DNA-binding transcriptional activator CadC; Provisional
 4-100 7.64e-06

DNA-binding transcriptional activator CadC; Provisional


Pssm-ID: 236658 [Multi-domain]  Cd Length: 517  Bit Score: 46.96  E-value: 7.64e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446454524   4 FLIGETIVfDPDRRIIYSENQTVRLHSSCVYCLELLIEHQGEIVEHEAFYEYVWRRfgMEVGPNALYQSISMLRKALSTc 83
Cdd:PRK10153   6 FRIGEWLV-TPSENKISRQGREVTLEPRLIDLLVFFAQHSGEVLSRDELIDHVWDR--AIVTDHVVTQSISELRKSLKD- 81

                         90
                 ....*....|....*....
gi 446454524  84 GTPDNI--ILTAPRRGFSL 100
Cdd:PRK10153  82 GREDNPeyIVTVPKRGYKL 100
OmpR COG0745
DNA-binding response regulator, OmpR family, contains REC and winged-helix (wHTH) domain ...
 11-92 2.79e-05

DNA-binding response regulator, OmpR family, contains REC and winged-helix (wHTH) domain [Signal transduction mechanisms, Transcription];


Pssm-ID: 440508 [Multi-domain]  Cd Length: 204  Bit Score: 44.18  E-value: 2.79e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446454524  11 VFDPDRRIIYSENQTVRLHSSCVYCLELLIEHQGEIVEHEAFYEYVWRRFgmEVGPNALYQSISMLRKALStcgtpDNII 90
Cdd:COG0745  130 LLDLAAREVTRDGEPVELTPKEFRLLELLMRNPGRVVSREQLLEEVWGYD--YGDDRTVDVHISRLRKKLE-----DDLI 202


                 ..
gi 446454524  91 LT 92
Cdd:COG0745  203 ET 204
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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