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Conserved domains on  [gi|446492427|ref|WP_000570281|]
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MULTISPECIES: GNAT family N-acetyltransferase [Bacillus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 47-132 1.39e-13

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


:

Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 62.54  E-value: 1.39e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427   47 GAYYLVVKKENNVMGWILIGENTDYFSHkklGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFAGNF-AK 124
Cdd:pfam00583  32 SEGFFVAEEDGELVGFASLSIIDDEPPV---GEIEGLAVAPEYRGKGIGTALLQALLEWArERGCERIFLEVAADNLaAI 108


                  ....*...
gi 446492427  125 EMYEELGF 132
Cdd:pfam00583 109 ALYEKLGF 116
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 47-132 1.39e-13

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 62.54  E-value: 1.39e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427   47 GAYYLVVKKENNVMGWILIGENTDYFSHkklGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFAGNF-AK 124
Cdd:pfam00583  32 SEGFFVAEEDGELVGFASLSIIDDEPPV---GEIEGLAVAPEYRGKGIGTALLQALLEWArERGCERIFLEVAADNLaAI 108


                  ....*...
gi 446492427  125 EMYEELGF 132
Cdd:pfam00583 109 ALYEKLGF 116
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 61-140 1.87e-12

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 59.28  E-value: 1.87e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427  61 GWILIGENTDyfshKKLGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFAGNF-AKEMYEELGFVERQVI 138
Cdd:COG0456    1 GFALLGLVDG----GDEAEIEDLAVDPEYRGRGIGRALLEAALERArERGARRLRLEVREDNEaAIALYEKLGFEEVGER 76


                 ..
gi 446492427 139 MT 140
Cdd:COG0456   77 PN 78
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 50-116 1.34e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 45.73  E-value: 1.34e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446492427  50 YLVVKKENNVMGWILIgenTDYFSHKKLGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLN 116
Cdd:cd04301    1 FLVAEDDGEIVGFASL---SPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEArERGAKRLRLE 65
PRK03624 PRK03624
putative acetyltransferase; Provisional
 78-139 1.26e-05

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 42.22  E-value: 1.26e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446492427  78 GFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFAGNFA-KEMYEELGFVERQVIM 139
Cdd:PRK03624  69 GWAYYLAVHPDFRGRGIGRALVARLEKKLiARGCPKINLQVREDNDAvLGFYEALGYEEQDRIS 132
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 47-132 1.39e-13

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 62.54  E-value: 1.39e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427   47 GAYYLVVKKENNVMGWILIGENTDYFSHkklGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFAGNF-AK 124
Cdd:pfam00583  32 SEGFFVAEEDGELVGFASLSIIDDEPPV---GEIEGLAVAPEYRGKGIGTALLQALLEWArERGCERIFLEVAADNLaAI 108


                  ....*...
gi 446492427  125 EMYEELGF 132
Cdd:pfam00583 109 ALYEKLGF 116
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 61-140 1.87e-12

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 59.28  E-value: 1.87e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427  61 GWILIGENTDyfshKKLGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFAGNF-AKEMYEELGFVERQVI 138
Cdd:COG0456    1 GFALLGLVDG----GDEAEIEDLAVDPEYRGRGIGRALLEAALERArERGARRLRLEVREDNEaAIALYEKLGFEEVGER 76


                 ..
gi 446492427 139 MT 140
Cdd:COG0456   77 PN 78
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
41-134 1.37e-11

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 58.47  E-value: 1.37e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427  41 RPIVEKGAYYLVVKKENNVMGWILIGentDYFSHKKLGFIYE--LYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNV 117
Cdd:COG1247   45 AAILAPGRPVLVAEEDGEVVGFASLG---PFRPRPAYRGTAEesIYVDPDARGRGIGRALLEALIERArARGYRRLVAVV 121

                         90
                 ....*....|....*...
gi 446492427 118 FAGN-FAKEMYEELGFVE 134
Cdd:COG1247  122 LADNeASIALYEKLGFEE 139
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 44-134 6.57e-10

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 53.43  E-value: 6.57e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427   44 VEKGAYYLVVKKENNVM-GWILIGENtdyfshkklGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFAGN 121
Cdd:pfam13673  26 IDQGEYFFFVAFEGGQIvGVIALRDR---------GHISLLFVDPDYQGQGIGKALLEAVEDYAeKDGIKLSELTVNASP 96

                          90
                  ....*....|...
gi 446492427  122 FAKEMYEELGFVE 134
Cdd:pfam13673  97 YAVPFYEKLGFRA 109
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
78-142 9.20e-10

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 51.83  E-value: 9.20e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446492427  78 GFIYELYVFPEYRGKGLSRKLMGAGIKE-LQKNYSEIRLNVFAGNF-AKEMYEELGFVERQVIMTLK 142
Cdd:COG3393   16 AEISGVYTHPEYRGRGLASALVAALAREaLARGARTPFLYVDADNPaARRLYERLGFRPVGEYATVL 82
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 46-134 1.02e-09

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 51.69  E-value: 1.02e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427   46 KGAYYLVVKKENNVMGWILIGENTDYFSHKKLGfiyeLYVFPEYRGKGLSRKLMGAgIKELQKNYSEIRLNVFAGNFAKE 125
Cdd:pfam13508   1 PGGRFFVAEDDGKIVGFAALLPLDDEGALAELR----LAVHPEYRGQGIGRALLEA-AEAAAKEGGIKLLELETTNRAAA 75


                  ....*....
gi 446492427  126 MYEELGFVE 134
Cdd:pfam13508  76 FYEKLGFEE 84
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
51-134 4.06e-09

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 51.62  E-value: 4.06e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427  51 LVVKKENNVMGWILIgENTDYFSHKKLGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLnvFAGNFAKEMYEE 129
Cdd:COG3153   42 LVAEDDGEIVGHVAL-SPVDIDGEGPALLLGPLAVDPEYRGQGIGRALMRAALEAArERGARAVVL--LGDPSLLPFYER 118


                 ....*
gi 446492427 130 LGFVE 134
Cdd:COG3153  119 FGFRP 123
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 41-134 4.41e-09

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 51.21  E-value: 4.41e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427  41 RPIVEKGAYYLVVKKENNVMGWILIGentdYFSHKKlGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFA 119
Cdd:COG0454   27 MEGSLAGAEFIAVDDKGEPIGFAGLR----RLDDKV-LELKRLYVLPEYRGKGIGKALLEALLEWArERGCTALELDTLD 101

                         90
                 ....*....|....*.
gi 446492427 120 GN-FAKEMYEELGFVE 134
Cdd:COG0454  102 GNpAAIRFYERLGFKE 117
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 43-134 1.83e-08

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 49.61  E-value: 1.83e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427  43 IVEKGAYYLVVKKENNVMGWILIgentdYFSHKKLGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLnvFAGN 121
Cdd:COG1246   23 LEEEIGEFWVAEEDGEIVGCAAL-----HPLDEDLAELRSLAVHPDYRGRGIGRRLLEALLAEArELGLKRLFL--LTTS 95

                         90
                 ....*....|...
gi 446492427 122 FAKEMYEELGFVE 134
Cdd:COG1246   96 AAIHFYEKLGFEE 108
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 50-116 1.34e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 45.73  E-value: 1.34e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446492427  50 YLVVKKENNVMGWILIgenTDYFSHKKLGFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLN 116
Cdd:cd04301    1 FLVAEDDGEIVGFASL---SPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEArERGAKRLRLE 65
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
87-134 5.31e-06

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 42.86  E-value: 5.31e-06
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 446492427  87 PEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFAGnfAKEMYEELGFVE 134
Cdd:COG2153   68 PEYRGQGLGRALMEAAIEEArERGARRIVLSAQAH--AVGFYEKLGFVP 114
PRK03624 PRK03624
putative acetyltransferase; Provisional
 78-139 1.26e-05

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 42.22  E-value: 1.26e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446492427  78 GFIYELYVFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFAGNFA-KEMYEELGFVERQVIM 139
Cdd:PRK03624  69 GWAYYLAVHPDFRGRGIGRALVARLEKKLiARGCPKINLQVREDNDAvLGFYEALGYEEQDRIS 132
FR47 pfam08445
FR47-like protein; The members of this family are similar to the C-terminal region of the D. ...
 74-135 6.02e-05

FR47-like protein; The members of this family are similar to the C-terminal region of the D. melanogaster hypothetical protein FR47. This protein has been found to consist of two N-acyltransferase-like domains swapped with the C-terminal strands.


Pssm-ID: 117022 [Multi-domain]  Cd Length: 86  Bit Score: 39.24  E-value: 6.02e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446492427   74 HKKLGFIYELYVFPEYRGKGLSRKLMGAGIKELQKNYSEIRLNVFAGNF-AKEMYEELGFVER 135
Cdd:pfam08445  18 RLPGGELGALQTLPEHRRRGLGSRLVAALARGIAERGITPFAVVVAGNTpSRRLYEKLGFRKI 80
rimI PRK09491
ribosomal-protein-alanine N-acetyltransferase; Provisional
 85-134 2.21e-03

ribosomal-protein-alanine N-acetyltransferase; Provisional


Pssm-ID: 181904 [Multi-domain]  Cd Length: 146  Bit Score: 36.06  E-value: 2.21e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 446492427  85 VFPEYRGKGLSRKLMGAGIKEL-QKNYSEIRLNVFAGNF-AKEMYEELGFVE 134
Cdd:PRK09491  71 VDPDYQRQGLGRALLEHLIDELeKRGVATLWLEVRASNAaAIALYESLGFNE 122
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 38-134 3.35e-03

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 35.75  E-value: 3.35e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427  38 EITRPIVEKGAYYLVV--KKENNVMGWILIGENTDYFSHKKLGFiyelYVFPEYRGKGLSRKLMGAGIKEL--QKNYSEI 113
Cdd:COG1670   50 RLLADWADGGALPFAIedKEDGELIGVVGLYDIDRANRSAEIGY----WLAPAYWGKGYATEALRALLDYAfeELGLHRV 125

                         90       100
                 ....*....|....*....|..
gi 446492427 114 RLNVFAGNFA-KEMYEELGFVE 134
Cdd:COG1670  126 EAEVDPDNTAsIRVLEKLGFRL 147
PRK07757 PRK07757
N-acetyltransferase;
50-134 5.43e-03

N-acetyltransferase;


Pssm-ID: 236088 [Multi-domain]  Cd Length: 152  Bit Score: 35.17  E-value: 5.43e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446492427  50 YLVVKKENNVMGWILIgentdYFSHKKLGFIYELYVFPEYRGKGLSRKLMGAGIKELQknysEIRLN-VFAGNFAKEMYE 128
Cdd:PRK07757  43 FYVAEEEGEIVGCCAL-----HILWEDLAEIRSLAVSEDYRGQGIGRMLVEACLEEAR----ELGVKrVFALTYQPEFFE 113


                 ....*.
gi 446492427 129 ELGFVE 134
Cdd:PRK07757 114 KLGFRE 119
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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