NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|446495972|ref|WP_000573826|]
View 

MULTISPECIES: iron-sulfur cluster assembly accessory protein [Bacillus]

Protein Classification

HesB/IscA family protein( domain architecture ID 10001059)

HesB/IscA family protein is a scaffold protein upon which 2Fe-2S clusters are assembled and subsequently transferred to acceptor proteins; similar to iron-sulfur assembly protein IscA that is involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway

Gene Ontology:  GO:0016226|GO:0051536
PubMed:  32108236

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 1-107 7.55e-50

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


:

Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 153.77  E-value: 7.55e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   1 MIEVTEQAAFQIKDMLKDAEDGEKYVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYK 80
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                         90       100
                 ....*....|....*....|....*..
gi 446495972  81 QSMLGGGFTIDNPNAIASCGCGSSFRT 107
Cdd:COG0316   81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 1-107 7.55e-50

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 153.77  E-value: 7.55e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   1 MIEVTEQAAFQIKDMLKDAEDGEKYVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYK 80
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                         90       100
                 ....*....|....*....|....*..
gi 446495972  81 QSMLGGGFTIDNPNAIASCGCGSSFRT 107
Cdd:COG0316   81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 3-107 6.32e-42

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 133.47  E-value: 6.32e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972    3 EVTEQAAFQIKDMLKDAEDGEKYVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYKQS 82
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPNLGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*
gi 446495972   83 MLGGGFTIDNPNAIASCGCGSSFRT 107
Cdd:TIGR00049  81 LLGSGFTFTNPNAKGTCGCGKSFSV 105
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 2-105 3.84e-27

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 96.53  E-value: 3.84e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   2 IEVTEQAAFQIKDMLKDAEDGEKYVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYKQ 81
Cdd:PRK13623  10 LVFTDAAAAKVKELIEEEGNPDLKLRVYITGGGCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGAEVDYTE 89

                         90       100
                 ....*....|....*....|....
gi 446495972  82 SMLGGGFTIDNPNAIASCGCGSSF 105
Cdd:PRK13623  90 GLEGSRFVIKNPNAKTTCGCGSSF 113
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 1-103 5.35e-17

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 70.05  E-value: 5.35e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   1 MIEVTEQAAFQIKDMLKDAEDgEKYVRLAVHGGGCSGLSYGLGFEvEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYk 80
Cdd:NF038090   1 MLEVTDLASRKLKEYFADKKI-DSPIRVFLNQGGUAGPSLGLALD-EPKENDEVFEQDGLTFVIDKELLEQCGPIKVDF- 77

                         90       100
                 ....*....|....*....|...
gi 446495972  81 qSMLGGGFTIDNPNAIASCGCGS 103
Cdd:NF038090  78 -IDCRSGFSITSSMPLGGGGCGS 99
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 2-102 1.26e-16

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 69.60  E-value: 1.26e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972    2 IEVTEQAAFQIKDMLkdaEDGEKYVRLAVHGGG-------CS-GLSYGLGFEVEP-KEDDTVLEFFGVEFVIDTESAP-I 71
Cdd:pfam01521   3 ITITDAAAERLKKLL---AGDKKELRLDVDDGGgpyskggCSiGGKFSLVLVDEPdPDYDEVIESNGGPIYVDSYSLPfL 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 446495972   72 VKGVKIDYKQSMLGGGFTIDNPNAIASCGCG 102
Cdd:pfam01521  80 DEGLTLDFVEDLGTLGLKSDNGNLDGNVGCG 110
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 1-107 7.55e-50

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 153.77  E-value: 7.55e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   1 MIEVTEQAAFQIKDMLKDAEDGEKYVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYK 80
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                         90       100
                 ....*....|....*....|....*..
gi 446495972  81 QSMLGGGFTIDNPNAIASCGCGSSFRT 107
Cdd:COG0316   81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 3-107 6.32e-42

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 133.47  E-value: 6.32e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972    3 EVTEQAAFQIKDMLKDAEDGEKYVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYKQS 82
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPNLGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*
gi 446495972   83 MLGGGFTIDNPNAIASCGCGSSFRT 107
Cdd:TIGR00049  81 LLGSGFTFTNPNAKGTCGCGKSFSV 105
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 2-105 3.84e-27

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 96.53  E-value: 3.84e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   2 IEVTEQAAFQIKDMLKDAEDGEKYVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYKQ 81
Cdd:PRK13623  10 LVFTDAAAAKVKELIEEEGNPDLKLRVYITGGGCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGAEVDYTE 89

                         90       100
                 ....*....|....*....|....
gi 446495972  82 SMLGGGFTIDNPNAIASCGCGSSF 105
Cdd:PRK13623  90 GLEGSRFVIKNPNAKTTCGCGSSF 113
sufA_proteo TIGR01997
FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of ...
 2-105 1.28e-23

FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of iron-sulfur cluster biosynthesis. This system performs FeS biosynthesis even during oxidative stress and tends to be absent in obligate anaerobic and microaerophilic bacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 131052  Cd Length: 107  Bit Score: 87.19  E-value: 1.28e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972    2 IEVTEQAAFQIKDMLKDAEDgEKYVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYKQ 81
Cdd:TIGR01997   3 ITLTDAAAIHIRELVAKRPE-AVGIRLGVKKTGCAGMEYVLDLVSEPKKDDDLIEHDGAKVFVAPEAVLFILGTQVDFVR 81

                          90       100
                  ....*....|....*....|....
gi 446495972   82 SMLGGGFTIDNPNAIASCGCGSSF 105
Cdd:TIGR01997  82 TTLRQGFKFNNPNATSACGCGESF 105
IscA TIGR02011
iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC ...
 2-105 5.74e-19

iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC system for iron-sulfur cluster assembly. The ISC system consists of IscRASU, HscAB and an Isc-specific ferredoxin. IscA previously was believed to act as a scaffold and now is seen as an iron donor protein. This clade is limited to the proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 213674  Cd Length: 105  Bit Score: 75.25  E-value: 5.74e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972    2 IEVTEQAAFQIKDMLKDAEDGEKyVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYKQ 81
Cdd:TIGR02011   1 ITLTDSAAARVNTFLANRGKGFG-LRLGVKTSGCSGMAYVLEFVDEPTPDDIVFEDKGVKIVIDGKSLQYLDGTQLDFVK 79

                          90       100
                  ....*....|....*....|....
gi 446495972   82 SMLGGGFTIDNPNAIASCGCGSSF 105
Cdd:TIGR02011  80 EGLNEGFKFTNPNVKDECGCGESF 103
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 2-105 4.67e-18

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 74.58  E-value: 4.67e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   2 IEVTEQAAFQIKDM-LKDAEDGEKYVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYK 80
Cdd:PLN03082  55 VHMTDNCIRRLKELqTSEPSAEDKMLRLSVETGGCSGFQYVFELDDKTNSDDRVFEKDGVKLVVDNISYDFVKGATVDYV 134

                         90       100
                 ....*....|....*....|....*.
gi 446495972  81 QSMLGGGFTI-DNPNAIASCGCGSSF 105
Cdd:PLN03082 135 EELIRSAFVVsTNPSAVGGCSCKSSF 160
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
2-105 6.58e-18

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 72.60  E-value: 6.58e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   2 IEVTEQAAFQIKDMLKDAEDGEKyVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYKQ 81
Cdd:PRK09502   3 ITLSDSAAARVNTFLANRGKGFG-LRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVK 81

                         90       100
                 ....*....|....*....|....
gi 446495972  82 SMLGGGFTIDNPNAIASCGCGSSF 105
Cdd:PRK09502  82 EGLNEGFKFTNPNVKDECGCGESF 105
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 1-103 5.35e-17

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 70.05  E-value: 5.35e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   1 MIEVTEQAAFQIKDMLKDAEDgEKYVRLAVHGGGCSGLSYGLGFEvEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYk 80
Cdd:NF038090   1 MLEVTDLASRKLKEYFADKKI-DSPIRVFLNQGGUAGPSLGLALD-EPKENDEVFEQDGLTFVIDKELLEQCGPIKVDF- 77

                         90       100
                 ....*....|....*....|...
gi 446495972  81 qSMLGGGFTIDNPNAIASCGCGS 103
Cdd:NF038090  78 -IDCRSGFSITSSMPLGGGGCGS 99
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 2-105 1.09e-16

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 70.15  E-value: 1.09e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   2 IEVTEQAAFQIKDmLKDAEDGEKYVRLAVHGGGCSGLSYGLGFEVEPKEDDTVLEFFGVEFVIDTESAPIVKGVKIDYKQ 81
Cdd:PRK09504  18 LTLTPAAAAHIRE-LMAKQPGMKGVRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAMPFIDGTEVDYVR 96

                         90       100
                 ....*....|....*....|....
gi 446495972  82 SMLGGGFTIDNPNAIASCGCGSSF 105
Cdd:PRK09504  97 EGLNQIFKFHNPKAQNECGCGESF 120
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 2-102 1.26e-16

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 69.60  E-value: 1.26e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972    2 IEVTEQAAFQIKDMLkdaEDGEKYVRLAVHGGG-------CS-GLSYGLGFEVEP-KEDDTVLEFFGVEFVIDTESAP-I 71
Cdd:pfam01521   3 ITITDAAAERLKKLL---AGDKKELRLDVDDGGgpyskggCSiGGKFSLVLVDEPdPDYDEVIESNGGPIYVDSYSLPfL 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 446495972   72 VKGVKIDYKQSMLGGGFTIDNPNAIASCGCG 102
Cdd:pfam01521  80 DEGLTLDFVEDLGTLGLKSDNGNLDGNVGCG 110
PRK11190 PRK11190
iron-sulfur cluster biogenesis protein NfuA;
1-95 6.19e-07

iron-sulfur cluster biogenesis protein NfuA;


Pssm-ID: 183027 [Multi-domain]  Cd Length: 192  Bit Score: 45.78  E-value: 6.19e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446495972   1 MIEVTEQAAFQIKDMLKDAEDGEKyVRLAVHGGGCS----GLSYGLGFEVEPkeDDTVLEFFGVEFVIDTESAPIVKGVK 76
Cdd:PRK11190   1 MITISDAAQAHFAKLLANQEEGTQ-IRVFVINPGTPnaecGVSYCPPDAVEA--TDTELKFDGFSAYVDELSAPFLEDAE 77

                         90
                 ....*....|....*....
gi 446495972  77 IDYKQSMLGGGFTIDNPNA 95
Cdd:PRK11190  78 IDFVTDQLGSQLTLKAPNA 96
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH