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Conserved domains on  [gi|446523884|ref|WP_000601230|]
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MULTISPECIES: GNAT family N-acetyltransferase [Bacillus]

Protein Classification

GNAT family protein( domain architecture ID 106742)

GNAT (Gcn5-related N-acetyltransferase) family protein similar to N-acetyltransferases that catalyze the transfer of an acetyl group from acetyl-CoA to a substrate

PubMed:  15581578

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Acetyltransf_10 super family cl48219
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 52-149 5.93e-12

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


The actual alignment was detected with superfamily member pfam13673:

Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 59.21  E-value: 5.93e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884   52 TFYGYFSENKLVGFISFVQEEKlidIHRLVVSPNFFQKGIATKLL----IYIFNMFPSSMAYIVqtgKANTPAITLYKKH 127
Cdd:pfam13673  32 FFFVAFEGGQIVGVIALRDRGH---ISLLFVDPDYQGQGIGKALLeaveDYAEKDGIKLSELTV---NASPYAVPFYEKL 105

                          90       100
                  ....*....|....*....|..
gi 446523884  128 GFITVSDTILPDGMILTQLKKT 149
Cdd:pfam13673 106 GFRATGPEQEFNGIRFVPMEKE 127
 
Name Accession Description Interval E-value
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 52-149 5.93e-12

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 59.21  E-value: 5.93e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884   52 TFYGYFSENKLVGFISFVQEEKlidIHRLVVSPNFFQKGIATKLL----IYIFNMFPSSMAYIVqtgKANTPAITLYKKH 127
Cdd:pfam13673  32 FFFVAFEGGQIVGVIALRDRGH---ISLLFVDPDYQGQGIGKALLeaveDYAEKDGIKLSELTV---NASPYAVPFYEKL 105

                          90       100
                  ....*....|....*....|..
gi 446523884  128 GFITVSDTILPDGMILTQLKKT 149
Cdd:pfam13673 106 GFRATGPEQEFNGIRFVPMEKE 127
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 64-135 1.11e-08

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 49.65  E-value: 1.11e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446523884  64 GFI--SFVQEEKLIDIHRLVVSPNFFQKGIATKLLIYIFNMFPSS-MAYI-VQTGKANTPAITLYKKHGFITVSDT 135
Cdd:COG0456    1 GFAllGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERgARRLrLEVREDNEAAIALYEKLGFEEVGER 76
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 53-96 5.50e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 41.88  E-value: 5.50e-06
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 446523884  53 FYGYFSENKLVGFISFVQEEKLID---IHRLVVSPNFFQKGIATKLL 96
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPDGSGGDtayIGDLAVLPEYRGKGIGSALL 47
PRK10562 PRK10562
putative acetyltransferase; Provisional
 56-129 7.02e-06

putative acetyltransferase; Provisional


Pssm-ID: 236715 [Multi-domain]  Cd Length: 145  Bit Score: 43.13  E-value: 7.02e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446523884  56 YFSENKLVGFISfVQEEKLIDIhrLVVSPNFFQKGIATKLLIYIFNMFPSSMAYIVQTgkaNTPAITLYKKHGF 129
Cdd:PRK10562  53 WEEDGKLLGFVS-VLEGRFVGA--LFVAPKAVRRGIGKALMQHVQQRYPHLSLEVYQK---NQRAVNFYHAQGF 120
 
Name Accession Description Interval E-value
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 52-149 5.93e-12

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 59.21  E-value: 5.93e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884   52 TFYGYFSENKLVGFISFVQEEKlidIHRLVVSPNFFQKGIATKLL----IYIFNMFPSSMAYIVqtgKANTPAITLYKKH 127
Cdd:pfam13673  32 FFFVAFEGGQIVGVIALRDRGH---ISLLFVDPDYQGQGIGKALLeaveDYAEKDGIKLSELTV---NASPYAVPFYEKL 105

                          90       100
                  ....*....|....*....|..
gi 446523884  128 GFITVSDTILPDGMILTQLKKT 149
Cdd:pfam13673 106 GFRATGPEQEFNGIRFVPMEKE 127
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 51-129 1.43e-11

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 57.91  E-value: 1.43e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884   51 ETFYGYFSENKLVGFISFV---QEEKLIDIHRLVVSPNFFQKGIATKLLIYIFNMFPSSMAYIV--QTGKANTPAITLYK 125
Cdd:pfam00583  33 EGFFVAEEDGELVGFASLSiidDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIflEVAADNLAAIALYE 112


                  ....
gi 446523884  126 KHGF 129
Cdd:pfam00583 113 KLGF 116
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 51-129 3.67e-10

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 53.23  E-value: 3.67e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884   51 ETFYGYFSENKLVGFISFVQ--EEKLIDIHRLVVSPNFFQKGIATKLLIYIFNMFPSSMAYIVQTgKANTPAITLYKKHG 128
Cdd:pfam13508   3 GRFFVAEDDGKIVGFAALLPldDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLEL-ETTNRAAAFYEKLG 81


                  .
gi 446523884  129 F 129
Cdd:pfam13508  82 F 82
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 64-135 1.11e-08

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 49.65  E-value: 1.11e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446523884  64 GFI--SFVQEEKLIDIHRLVVSPNFFQKGIATKLLIYIFNMFPSS-MAYI-VQTGKANTPAITLYKKHGFITVSDT 135
Cdd:COG0456    1 GFAllGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERgARRLrLEVREDNEAAIALYEKLGFEEVGER 76
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 44-141 2.66e-07

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 46.97  E-value: 2.66e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884  44 NDIQNCVETFYGYFS-----ENKLVGFISFVQ-EEKLIDIHRLVVSPNFFQKGIATKLLIYIfnmfpSSMA-------YI 110
Cdd:COG0454   22 AELKAMEGSLAGAEFiavddKGEPIGFAGLRRlDDKVLELKRLYVLPEYRGKGIGKALLEAL-----LEWArergctaLE 96

                         90       100       110
                 ....*....|....*....|....*....|.
gi 446523884 111 VQTGKANTPAITLYKKHGFITVSDTILPDGM 141
Cdd:COG0454   97 LDTLDGNPAAIRFYERLGFKEIERYVAYVGG 127
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 51-140 5.29e-07

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 46.14  E-value: 5.29e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884  51 ETFYGYFSENKLVGFISF-VQEEKLIDIHRLVVSPNFFQKGIATKLliyifnmfpssMAYIVQTGKA----------NTP 119
Cdd:COG1246   28 GEFWVAEEDGEIVGCAALhPLDEDLAELRSLAVHPDYRGRGIGRRL-----------LEALLAEARElglkrlflltTSA 96

                         90       100
                 ....*....|....*....|.
gi 446523884 120 AITLYKKHGFITVSDTILPDG 140
Cdd:COG1246   97 AIHFYEKLGFEEIDKEDLPYA 117
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 53-96 5.50e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 41.88  E-value: 5.50e-06
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 446523884  53 FYGYFSENKLVGFISFVQEEKLID---IHRLVVSPNFFQKGIATKLL 96
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPDGSGGDtayIGDLAVLPEYRGKGIGSALL 47
PRK10562 PRK10562
putative acetyltransferase; Provisional
 56-129 7.02e-06

putative acetyltransferase; Provisional


Pssm-ID: 236715 [Multi-domain]  Cd Length: 145  Bit Score: 43.13  E-value: 7.02e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446523884  56 YFSENKLVGFISfVQEEKLIDIhrLVVSPNFFQKGIATKLLIYIFNMFPSSMAYIVQTgkaNTPAITLYKKHGF 129
Cdd:PRK10562  53 WEEDGKLLGFVS-VLEGRFVGA--LFVAPKAVRRGIGKALMQHVQQRYPHLSLEVYQK---NQRAVNFYHAQGF 120
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
54-144 1.78e-04

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 39.01  E-value: 1.78e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884  54 YGYFSENKLVGFISFVQEE-KLIDIHRLVVSPNFFQKGIATKLLIYIfnmfpssMAYIVQTGK------ANTPAITLYKK 126
Cdd:COG2153   37 LLAYDDGELVATARLLPPGdGEAKIGRVAVLPEYRGQGLGRALMEAA-------IEEARERGArrivlsAQAHAVGFYEK 109

                         90       100
                 ....*....|....*....|...
gi 446523884 127 HGFITVSDTILPDG-----MILT 144
Cdd:COG2153  110 LGFVPVGEEFLEAGiphidMRKP 132
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
59-132 1.19e-03

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 37.28  E-value: 1.19e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884  59 ENKLVGFISFVQ-----EEKLIDIHRLVVSPNFFQKGIATKLLIYIFNMFPS----SMayIVQTGKANTPAITLYKKHGF 129
Cdd:COG1247   60 DGEVVGFASLGPfrprpAYRGTAEESIYVDPDARGRGIGRALLEALIERARArgyrRL--VAVVLADNEASIALYEKLGF 137


                 ...
gi 446523884 130 ITV 132
Cdd:COG1247  138 EEV 140
YidJ COG2388
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
59-96 3.97e-03

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 441953 [Multi-domain]  Cd Length: 88  Bit Score: 34.75  E-value: 3.97e-03
                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 446523884  59 ENKLVGFISFVQEEKLIDIHRLVVSPNFFQKGIATKLL 96
Cdd:COG2388   17 DGELAGELTYRLEGGVIIITHTEVPPALRGQGIASALV 54
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
59-140 4.46e-03

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 35.45  E-value: 4.46e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884  59 ENKLVGFISF-----VQEEKLIDIHRLVVSPNFFQKGIATKLLIYIFNMFPSSMAYIVQTGkANTPAITLYKKHGFITVS 133
Cdd:COG3153   47 DGEIVGHVALspvdiDGEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLL-GDPSLLPFYERFGFRPAG 125


                 ....*..
gi 446523884 134 DTILPDG 140
Cdd:COG3153  126 ELGLTLG 132
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
77-134 5.60e-03

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 34.11  E-value: 5.60e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446523884  77 IHRLVVSPNFFQKGIATKLLIYIFNMFPSSMAYIV--QTGKANTPAITLYKKHGFITVSD 134
Cdd:COG3393   18 ISGVYTHPEYRGRGLASALVAALAREALARGARTPflYVDADNPAARRLYERLGFRPVGE 77
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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