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Conserved domains on  [gi|446528024|ref|WP_000605370|]
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type III secretion system LEE GrlA-binding negative regulator GrlR [Escherichia coli]

Protein Classification

PRK14051 family protein( domain architecture ID 10014551)

PRK14051 family protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK14051 PRK14051
negative regulator GrlR; Provisional
 1-123 7.42e-78

negative regulator GrlR; Provisional


:

Pssm-ID: 184476  Cd Length: 123  Bit Score: 225.26  E-value: 7.42e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446528024   1 MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVLSEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVL 80
Cdd:PRK14051   1 MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVLSEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 446528024  81 SNDNNLTLHCHVRGNEKLFVDVYAKFIEPLVIKNTGMPQVYLK 123
Cdd:PRK14051  81 SNDNNLTLHCHVRGNEKLFVDVYAKFIEPLVIKNTGMPQVYLK 123
 
Name Accession Description Interval E-value
PRK14051 PRK14051
negative regulator GrlR; Provisional
 1-123 7.42e-78

negative regulator GrlR; Provisional


Pssm-ID: 184476  Cd Length: 123  Bit Score: 225.26  E-value: 7.42e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446528024   1 MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVLSEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVL 80
Cdd:PRK14051   1 MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVLSEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 446528024  81 SNDNNLTLHCHVRGNEKLFVDVYAKFIEPLVIKNTGMPQVYLK 123
Cdd:PRK14051  81 SNDNNLTLHCHVRGNEKLFVDVYAKFIEPLVIKNTGMPQVYLK 123
GrlR pfam16518
T3SS negative regulator,GrlR; GrlR is a family of protobacterial type III secretion system ...
 3-65 1.10e-04

T3SS negative regulator,GrlR; GrlR is a family of protobacterial type III secretion system negative regulators. Structurally, GrlR consists of a typical beta-barrel fold with eight beta-strands containing an internal hydrophobic cavity and a plug-like loop on one side of the barrel. Strong hydrophobic interactions between the two beta-barrels maintain its dimeric architecture. A unique surface-exposed EDED (Glu-Asp-Glu-Asp) motif is identified to be critical for GrlA-GrlR interaction and for the repressive activity of GrlR. The locus of enterocyte effacement (LEE) is essential for virulence of enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC). It encodes some 20 genes including an overall regulator ler and two others, GrlR and GrlA, that form the type three secretion system for infection. GrlR comlexes with GrlA to repress expression of ler. GrlA is found in family pfam00462.


Pssm-ID: 406825  Cd Length: 112  Bit Score: 38.74  E-value: 1.10e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446528024    3 MKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVLSEDEDII---LHVHRYNYEIPSVL 65
Cdd:pfam16518   1 MADGIYFIKFKAIDGGLEEGVAVFDDGLLHGGDPGYVWQGRLAAHDGAMramIEVRKWNGPADPAF 66
 
Name Accession Description Interval E-value
PRK14051 PRK14051
negative regulator GrlR; Provisional
 1-123 7.42e-78

negative regulator GrlR; Provisional


Pssm-ID: 184476  Cd Length: 123  Bit Score: 225.26  E-value: 7.42e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446528024   1 MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVLSEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVL 80
Cdd:PRK14051   1 MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVLSEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 446528024  81 SNDNNLTLHCHVRGNEKLFVDVYAKFIEPLVIKNTGMPQVYLK 123
Cdd:PRK14051  81 SNDNNLTLHCHVRGNEKLFVDVYAKFIEPLVIKNTGMPQVYLK 123
GrlR pfam16518
T3SS negative regulator,GrlR; GrlR is a family of protobacterial type III secretion system ...
 3-65 1.10e-04

T3SS negative regulator,GrlR; GrlR is a family of protobacterial type III secretion system negative regulators. Structurally, GrlR consists of a typical beta-barrel fold with eight beta-strands containing an internal hydrophobic cavity and a plug-like loop on one side of the barrel. Strong hydrophobic interactions between the two beta-barrels maintain its dimeric architecture. A unique surface-exposed EDED (Glu-Asp-Glu-Asp) motif is identified to be critical for GrlA-GrlR interaction and for the repressive activity of GrlR. The locus of enterocyte effacement (LEE) is essential for virulence of enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC). It encodes some 20 genes including an overall regulator ler and two others, GrlR and GrlA, that form the type three secretion system for infection. GrlR comlexes with GrlA to repress expression of ler. GrlA is found in family pfam00462.


Pssm-ID: 406825  Cd Length: 112  Bit Score: 38.74  E-value: 1.10e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446528024    3 MKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVLSEDEDII---LHVHRYNYEIPSVL 65
Cdd:pfam16518   1 MADGIYFIKFKAIDGGLEEGVAVFDDGLLHGGDPGYVWQGRLAAHDGAMramIEVRKWNGPADPAF 66
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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