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Conserved domains on  [gi|446554892|ref|WP_000632238|]
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MULTISPECIES: GNAT family N-acetyltransferase [Bacillus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 707069)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate; similar to Bacillus subtilis protein YyaT

CATH:  3.40.630.30
Gene Ontology:  GO:0008080
SCOP:  4001800

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ElaA super family cl28579
Predicted N-acyltransferase, GNAT family [General function prediction only];
172-250 8.17e-09

Predicted N-acyltransferase, GNAT family [General function prediction only];


The actual alignment was detected with superfamily member COG2153:

Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 52.88  E-value: 8.17e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446554892 172 FIGCIDDMPVTTG-LLIESKDSYGIYDVMTKETFRGKGIGSEMFQFLLNQTTDKQ-KPVVLQASDDGKNIYKRFGFIDVG 249
Cdd:COG2153   37 LLAYDDGELVATArLLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGaRRIVLSAQAHAVGFYEKLGFVPVG 116


                 .
gi 446554892 250 E 250
Cdd:COG2153  117 E 117
 
Name Accession Description Interval E-value
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
172-250 8.17e-09

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 52.88  E-value: 8.17e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446554892 172 FIGCIDDMPVTTG-LLIESKDSYGIYDVMTKETFRGKGIGSEMFQFLLNQTTDKQ-KPVVLQASDDGKNIYKRFGFIDVG 249
Cdd:COG2153   37 LLAYDDGELVATArLLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGaRRIVLSAQAHAVGFYEKLGFVPVG 116


                 .
gi 446554892 250 E 250
Cdd:COG2153  117 E 117
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 170-245 5.34e-05

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 40.90  E-value: 5.34e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446554892  170 QMFIGCIDDMPVTTGLLIESKDSYGIY--DVMTKETFRGKGIGSEMFQFLLNQTTDKQ-KPVVLQASDDGKNIYKRFGF 245
Cdd:pfam13508   4 RFFVAEDDGKIVGFAALLPLDDEGALAelRLAVHPEYRGQGIGRALLEAAEAAAKEGGiKLLELETTNRAAAFYEKLGF 82
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 171-220 1.64e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 36.10  E-value: 1.64e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 446554892 171 MFIGCIDDMPVTTGLLI---ESKDSYGIYDVMTKETFRGKGIGSEMFQFLLNQ 220
Cdd:cd04301    1 FLVAEDDGEIVGFASLSpdgSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEE 53
PTZ00330 PTZ00330
acetyltransferase; Provisional
 182-245 6.24e-03

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 36.36  E-value: 6.24e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446554892 182 TTGLLIESKDSYG------IYDVMTKETFRGKGIGSEMFQFLLNQTTDKQ-KPVVLQASDDGKNIYKRFGF 245
Cdd:PTZ00330  66 TASLFVEPKFTRGgkcvghIEDVVVDPSYRGQGLGRALISDLCEIARSSGcYKVILDCTEDMVAFYKKLGF 136
 
Name Accession Description Interval E-value
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
172-250 8.17e-09

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 52.88  E-value: 8.17e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446554892 172 FIGCIDDMPVTTG-LLIESKDSYGIYDVMTKETFRGKGIGSEMFQFLLNQTTDKQ-KPVVLQASDDGKNIYKRFGFIDVG 249
Cdd:COG2153   37 LLAYDDGELVATArLLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGaRRIVLSAQAHAVGFYEKLGFVPVG 116


                 .
gi 446554892 250 E 250
Cdd:COG2153  117 E 117
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
173-253 1.93e-06

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 46.23  E-value: 1.93e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446554892 173 IGCIDDMPVTtglLIESKDSYGIYDVMTKETFRGKGIGSEMFQFLLNQTTDKQ-KPVVLQASDDGKNIYKRFGFIDVGEM 251
Cdd:COG3153   51 VGHVALSPVD---IDGEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGaRAVVLLGDPSLLPFYERFGFRPAGEL 127


                 ..
gi 446554892 252 VV 253
Cdd:COG3153  128 GL 129
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 195-254 2.83e-06

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 44.65  E-value: 2.83e-06
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446554892 195 IYDVMTKETFRGKGIGSEMFQFLLNQTTDKQKPV----VLQASDDGKNIYKRFGFIDVGEMVVF 254
Cdd:COG0456   16 IEDLAVDPEYRGRGIGRALLEAALERARERGARRlrleVREDNEAAIALYEKLGFEEVGERPNY 79
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 173-250 1.50e-05

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 43.44  E-value: 1.50e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446554892 173 IGCIddmpvttGLLIESKDSYGIYDVMTKETFRGKGIGSEMFQFLLNQTTDKQ-KPVVLQASDDGKNIYKRFGFIDVGE 250
Cdd:COG1246   40 VGCA-------ALHPLDEDLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGlKRLFLLTTSAAIHFYEKLGFEEIDK 111
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 170-245 5.34e-05

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 40.90  E-value: 5.34e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446554892  170 QMFIGCIDDMPVTTGLLIESKDSYGIY--DVMTKETFRGKGIGSEMFQFLLNQTTDKQ-KPVVLQASDDGKNIYKRFGF 245
Cdd:pfam13508   4 RFFVAEDDGKIVGFAALLPLDDEGALAelRLAVHPEYRGQGIGRALLEAAEAAAKEGGiKLLELETTNRAAAFYEKLGF 82
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 167-255 7.53e-05

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 41.49  E-value: 7.53e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446554892  167 SEVQMFIGCIDDMPVttGLlIESKDSYGIYDVMTKETFRGKGIGSEMFQFLLNQTtDKQKP----VVLQASDDGKNIYKR 242
Cdd:pfam13673  29 GEYFFFVAFEGGQIV--GV-IALRDRGHISLLFVDPDYQGQGIGKALLEAVEDYA-EKDGIklseLTVNASPYAVPFYEK 104

                          90
                  ....*....|...
gi 446554892  243 FGFIDVGEMVVFE 255
Cdd:pfam13673 105 LGFRATGPEQEFN 117
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
184-255 1.21e-04

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 39.89  E-value: 1.21e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446554892 184 GLLIESKDSYGIYDVMTKETFRGKGIGSEMFQFLLNQTTDKQKPV----VLQASDDGKNIYKRFGFIDVGE--MVVFE 255
Cdd:COG3393    7 GVRAESPGVAEISGVYTHPEYRGRGLASALVAALAREALARGARTpflyVDADNPAARRLYERLGFRPVGEyaTVLFR 84
Eis COG4552
Predicted acetyltransferase [General function prediction only];
131-245 1.36e-04

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 42.58  E-value: 1.36e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446554892 131 EELLKYIDVFMSLFEGTPEKDALESYFNKFSQVKlgsevqmFIGCIDD-MPVTTGLLIESK--------DSYGIYDVMTK 201
Cdd:COG4552    9 DDLDAFARLLAYAFGPEPDDEELEAYRPLLEPGR-------VLGVFDDgELVGTLALYPFTlnvggarvPMAGITGVAVA 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 446554892 202 ETFRGKGIGSEMFQFLLNQTTDKQKPV-VLQASDDGknIYKRFGF 245
Cdd:COG4552   82 PEHRRRGVARALLREALAELRERGQPLsALYPFEPG--FYRRFGY 124
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 131-245 1.15e-03

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 37.94  E-value: 1.15e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446554892  131 EELLKYIDVFMSLFEGTPEKDALESYFNKF--SQVKLGSEVQMFIGCIDDMPVTTGLLIESKDSYGIYDVMTKETFRGKG 208
Cdd:pfam13527   7 DEFDEVLRLLEYAFQDEDSPELREYFRPLLeeGRVLGAFDDGELVSTLALYPFELNVPGKTLPAAGITGVATYPEYRGRG 86

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 446554892  209 IGSEMFQFLLNQTTDKQKPVV-LQASDDGknIYKRFGF 245
Cdd:pfam13527  87 VMSRLLRRSLEEMRERGVPLSfLYPSSYP--IYRRFGY 122
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 171-220 1.64e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 36.10  E-value: 1.64e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 446554892 171 MFIGCIDDMPVTTGLLI---ESKDSYGIYDVMTKETFRGKGIGSEMFQFLLNQ 220
Cdd:cd04301    1 FLVAEDDGEIVGFASLSpdgSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEE 53
PTZ00330 PTZ00330
acetyltransferase; Provisional
 182-245 6.24e-03

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 36.36  E-value: 6.24e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446554892 182 TTGLLIESKDSYG------IYDVMTKETFRGKGIGSEMFQFLLNQTTDKQ-KPVVLQASDDGKNIYKRFGF 245
Cdd:PTZ00330  66 TASLFVEPKFTRGgkcvghIEDVVVDPSYRGQGLGRALISDLCEIARSSGcYKVILDCTEDMVAFYKKLGF 136
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 137-245 9.01e-03

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 35.19  E-value: 9.01e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446554892  137 IDVFMSLFEGTPEKDALESYFNKFSQVKLGSEVQMFIGCIDDMPV--TTGLLIESKDSYG-IYDVMTKETFRGKGIGSEM 213
Cdd:pfam00583   1 LEALYELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVgfASLSIIDDEPPVGeIEGLAVAPEYRGKGIGTAL 80

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 446554892  214 FQFLLNQTTDKQ-KPVVLQASDDGK---NIYKRFGF 245
Cdd:pfam00583  81 LQALLEWARERGcERIFLEVAADNLaaiALYEKLGF 116
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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