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Conserved domains on  [gi|446556992|ref|WP_000634338|]
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MULTISPECIES: K5 polysaccharide biosynthesis alpha-UDP-GlcNAc glycosyltransferase KfiA [Enterobacteriaceae]

Protein Classification

glycosyltransferase family protein( domain architecture ID 27718)

glycosyltransferase family protein may synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Glyco_tranf_GTA_type super family cl11394
Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a ...
 9-154 3.50e-05

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.


The actual alignment was detected with superfamily member cd02526:

Pssm-ID: 472172 [Multi-domain]  Cd Length: 237  Bit Score: 43.43  E-value: 3.50e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446556992   9 YPPRKKELVHSIQSLHAQVDKINLCLNEFEEIPEELDGFSKLNPVipdkdykdvgkfIFPCAKN---------------- 72
Cdd:cd02526    6 YNPDLSKLKELLAALAEQVDKVVVVDNSSGNDIELRLRLNSEKIE------------LIHLGENlgiakalnigikaale 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446556992  73 ---DMIVLTDDDIIYPPDYVEKMLNFYnsFAIFNCI-VGIHGCIYIDAFDGDQSKRKVFSftqgllRPRVVNQLGTGTVF 148
Cdd:cd02526   74 ngaDYVLLFDQDSVPPPDMVEKLLAYK--ILSDKNSnIGAVGPRIIDRRTGENSPGVRKS------GYKLRIQKEGEEGL 145


                 ....*.
gi 446556992 149 LKADQL 154
Cdd:cd02526  146 KEVDFL 151
 
Name Accession Description Interval E-value
GT2_RfbF_like cd02526
RfbF is a putative dTDP-rhamnosyl transferase; Shigella flexneri RfbF protein is a putative ...
 9-154 3.50e-05

RfbF is a putative dTDP-rhamnosyl transferase; Shigella flexneri RfbF protein is a putative dTDP-rhamnosyl transferase. dTDP rhamnosyl transferases of Shigella flexneri add rhamnose sugars to N-acetyl-glucosamine in the O-antigen tetrasaccharide repeat. Lipopolysaccharide O antigens are important virulence determinants for many bacteria. The variations of sugar composition, the sequence of the sugars and the linkages in the O antigen provide structural diversity of the O antigen.


Pssm-ID: 133017 [Multi-domain]  Cd Length: 237  Bit Score: 43.43  E-value: 3.50e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446556992   9 YPPRKKELVHSIQSLHAQVDKINLCLNEFEEIPEELDGFSKLNPVipdkdykdvgkfIFPCAKN---------------- 72
Cdd:cd02526    6 YNPDLSKLKELLAALAEQVDKVVVVDNSSGNDIELRLRLNSEKIE------------LIHLGENlgiakalnigikaale 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446556992  73 ---DMIVLTDDDIIYPPDYVEKMLNFYnsFAIFNCI-VGIHGCIYIDAFDGDQSKRKVFSftqgllRPRVVNQLGTGTVF 148
Cdd:cd02526   74 ngaDYVLLFDQDSVPPPDMVEKLLAYK--ILSDKNSnIGAVGPRIIDRRTGENSPGVRKS------GYKLRIQKEGEEGL 145


                 ....*.
gi 446556992 149 LKADQL 154
Cdd:cd02526  146 KEVDFL 151
BcsA COG1215
Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1, ...
 70-118 4.00e-03

Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility];


Pssm-ID: 440828 [Multi-domain]  Cd Length: 303  Bit Score: 37.80  E-value: 4.00e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 446556992  70 AKNDMIVLTDDDIIYPPDYVEKMLNFYNSFAifnciVGIHGCIYI---DAFD 118
Cdd:COG1215  110 ARGDIVVFLDADTVLDPDWLRRLVAAFADPG-----VGASGANLAfrrEALE 156
 
Name Accession Description Interval E-value
GT2_RfbF_like cd02526
RfbF is a putative dTDP-rhamnosyl transferase; Shigella flexneri RfbF protein is a putative ...
 9-154 3.50e-05

RfbF is a putative dTDP-rhamnosyl transferase; Shigella flexneri RfbF protein is a putative dTDP-rhamnosyl transferase. dTDP rhamnosyl transferases of Shigella flexneri add rhamnose sugars to N-acetyl-glucosamine in the O-antigen tetrasaccharide repeat. Lipopolysaccharide O antigens are important virulence determinants for many bacteria. The variations of sugar composition, the sequence of the sugars and the linkages in the O antigen provide structural diversity of the O antigen.


Pssm-ID: 133017 [Multi-domain]  Cd Length: 237  Bit Score: 43.43  E-value: 3.50e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446556992   9 YPPRKKELVHSIQSLHAQVDKINLCLNEFEEIPEELDGFSKLNPVipdkdykdvgkfIFPCAKN---------------- 72
Cdd:cd02526    6 YNPDLSKLKELLAALAEQVDKVVVVDNSSGNDIELRLRLNSEKIE------------LIHLGENlgiakalnigikaale 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446556992  73 ---DMIVLTDDDIIYPPDYVEKMLNFYnsFAIFNCI-VGIHGCIYIDAFDGDQSKRKVFSftqgllRPRVVNQLGTGTVF 148
Cdd:cd02526   74 ngaDYVLLFDQDSVPPPDMVEKLLAYK--ILSDKNSnIGAVGPRIIDRRTGENSPGVRKS------GYKLRIQKEGEEGL 145


                 ....*.
gi 446556992 149 LKADQL 154
Cdd:cd02526  146 KEVDFL 151
BcsA COG1215
Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1, ...
 70-118 4.00e-03

Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility];


Pssm-ID: 440828 [Multi-domain]  Cd Length: 303  Bit Score: 37.80  E-value: 4.00e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 446556992  70 AKNDMIVLTDDDIIYPPDYVEKMLNFYNSFAifnciVGIHGCIYI---DAFD 118
Cdd:COG1215  110 ARGDIVVFLDADTVLDPDWLRRLVAAFADPG-----VGASGANLAfrrEALE 156
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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