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Conserved domains on  [gi|446596617|ref|WP_000673963|]
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MULTISPECIES: GNAT family N-acetyltransferase [Bacillus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 12001312)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 87-170 4.41e-09

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 51.19  E-value: 4.41e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617  87 EWLQDCFEFVELAVHPQYQNEGLGTKLHNELLEGIPNR---TSVLTTQINNEKARSLYERLDWINILEpfHPSTNDVPYV 163
Cdd:COG0456    8 VDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERgarRLRLEVREDNEAAIALYEKLGFEEVGE--RPNYYGDDAL 85


                 ....*..
gi 446596617 164 IMGKALK 170
Cdd:COG0456   86 VMEKELA 92
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 15-144 5.64e-06

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


:

Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 43.28  E-value: 5.64e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617   15 DMAKLYCKVFEKInFNEIIERMNRHIEYTGFKGIVAINDENEVVGFTYGYrsmegqyynqlmrealhleqVDEWLQDCFE 94
Cdd:pfam00583   3 ALYELLSEEFPEP-WPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLS--------------------IIDDEPPVGE 61

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 446596617   95 FVELAVHPQYQNEGLGTKLHNELLE-----GIpnRTSVLTTQINNEKARSLYERL 144
Cdd:pfam00583  62 IEGLAVAPEYRGKGIGTALLQALLEwarerGC--ERIFLEVAADNLAAIALYEKL 114
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 87-170 4.41e-09

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 51.19  E-value: 4.41e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617  87 EWLQDCFEFVELAVHPQYQNEGLGTKLHNELLEGIPNR---TSVLTTQINNEKARSLYERLDWINILEpfHPSTNDVPYV 163
Cdd:COG0456    8 VDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERgarRLRLEVREDNEAAIALYEKLGFEEVGE--RPNYYGDDAL 85


                 ....*..
gi 446596617 164 IMGKALK 170
Cdd:COG0456   86 VMEKELA 92
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 85-144 3.19e-06

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 43.21  E-value: 3.19e-06
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617   85 VDEWLQDCFEFVELAVHPQYQNEGLGTKLHNELLEGIPNRTSVLTTQINNEKARSLYERL 144
Cdd:pfam13508  21 LPLDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNRAAAFYEKL 80
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 15-144 5.64e-06

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 43.28  E-value: 5.64e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617   15 DMAKLYCKVFEKInFNEIIERMNRHIEYTGFKGIVAINDENEVVGFTYGYrsmegqyynqlmrealhleqVDEWLQDCFE 94
Cdd:pfam00583   3 ALYELLSEEFPEP-WPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLS--------------------IIDDEPPVGE 61

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 446596617   95 FVELAVHPQYQNEGLGTKLHNELLE-----GIpnRTSVLTTQINNEKARSLYERL 144
Cdd:pfam00583  62 IEGLAVAPEYRGKGIGTALLQALLEwarerGC--ERIFLEVAADNLAAIALYEKL 114
rimI PRK09491
ribosomal-protein-alanine N-acetyltransferase; Provisional
 98-144 2.61e-04

ribosomal-protein-alanine N-acetyltransferase; Provisional


Pssm-ID: 181904 [Multi-domain]  Cd Length: 146  Bit Score: 39.14  E-value: 2.61e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 446596617  98 LAVHPQYQNEGLGTKLHNELLEGIPNR---TSVLTTQINNEKARSLYERL 144
Cdd:PRK09491  69 IAVDPDYQRQGLGRALLEHLIDELEKRgvaTLWLEVRASNAAAIALYESL 118
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 48-119 5.80e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 36.48  E-value: 5.80e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446596617  48 IVAINDENEVVGFTYGYRsmegqyynqlmrealhleqvDEWLQDCFEFVELAVHPQYQNEGLGTKLHNELLE 119
Cdd:cd04301    1 FLVAEDDGEIVGFASLSP--------------------DGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEE 52
rimI TIGR01575
ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related ...
 98-144 6.78e-04

ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related N-acetyltransferase (GNAT) superfamily. This model covers prokarotes and the archaea. The seed contains a characterized accession for Gram negative E. coli. An untraceable characterized accession (PIR|S66013) for Gram positive B. subtilis scores well (205.0) in the full alignment. Characterized members are lacking in the archaea. Noise cutoff (72.4) was set to exclude M. loti paralog of rimI. Trusted cutoff (80.0) was set at next highest scoring member in the mini-database. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 273701 [Multi-domain]  Cd Length: 131  Bit Score: 37.69  E-value: 6.78e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 446596617   98 LAVHPQYQNEGLGTKLHNEL---LEGIPNRTSVLTTQINNEKARSLYERL 144
Cdd:TIGR01575  60 IAVKPEYQGQGIGRALLRELideAKGRGVNEIFLEVRVSNIAAQALYKKL 109
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 87-170 4.41e-09

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 51.19  E-value: 4.41e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617  87 EWLQDCFEFVELAVHPQYQNEGLGTKLHNELLEGIPNR---TSVLTTQINNEKARSLYERLDWINILEpfHPSTNDVPYV 163
Cdd:COG0456    8 VDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERgarRLRLEVREDNEAAIALYEKLGFEEVGE--RPNYYGDDAL 85


                 ....*..
gi 446596617 164 IMGKALK 170
Cdd:COG0456   86 VMEKELA 92
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
13-169 1.58e-07

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 48.16  E-value: 1.58e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617  13 IGDMAKLYCKVFEKINFNEIIERMNRHIEYTGFkgIVAInDENEVVGF-TYGYRSMEGQyynqlmREALHLEqvdewlqd 91
Cdd:COG3153    9 AEAIAALLRAAFGPGREAELVDRLREDPAAGLS--LVAE-DDGEIVGHvALSPVDIDGE------GPALLLG-------- 71

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617  92 cfefvELAVHPQYQNEGLGTKLHNELLE-----GIpnRTSVLTTqinNEKARSLYERLDWINILEPFHPStnDVPYVIMG 166
Cdd:COG3153   72 -----PLAVDPEYRGQGIGRALMRAALEaarerGA--RAVVLLG---DPSLLPFYERFGFRPAGELGLTL--GPDEVFLA 139


                 ...
gi 446596617 167 KAL 169
Cdd:COG3153  140 KEL 142
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
31-146 1.55e-06

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 45.76  E-value: 1.55e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617  31 EIIERMNRHIEYTGFKGIVAiNDENEVVGFTYGYRSMEGQYYNQLMREALHleqvdewlqdcfefvelaVHPQYQNEGLG 110
Cdd:COG1247   38 EEREAWFAAILAPGRPVLVA-EEDGEVVGFASLGPFRPRPAYRGTAEESIY------------------VDPDARGRGIG 98

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 446596617 111 TKLHNELLE-----GIpnRTSVLTTQINNEKARSLYERLDW 146
Cdd:COG1247   99 RALLEALIErararGY--RRLVAVVLADNEASIALYEKLGF 137
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 85-144 3.19e-06

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 43.21  E-value: 3.19e-06
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617   85 VDEWLQDCFEFVELAVHPQYQNEGLGTKLHNELLEGIPNRTSVLTTQINNEKARSLYERL 144
Cdd:pfam13508  21 LPLDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNRAAAFYEKL 80
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 49-144 4.44e-06

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 43.89  E-value: 4.44e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617  49 VAINDENEVVGFtYGYRSMEGqyynqlmrEALHLEQvdewlqdcfefveLAVHPQYQNEGLGTKLHNELLE-----GIpn 123
Cdd:COG0454   37 IAVDDKGEPIGF-AGLRRLDD--------KVLELKR-------------LYVLPEYRGKGIGKALLEALLEwarerGC-- 92

                         90       100
                 ....*....|....*....|.
gi 446596617 124 RTSVLTTQINNEKARSLYERL 144
Cdd:COG0454   93 TALELDTLDGNPAAIRFYERL 113
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 15-144 5.64e-06

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 43.28  E-value: 5.64e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617   15 DMAKLYCKVFEKInFNEIIERMNRHIEYTGFKGIVAINDENEVVGFTYGYrsmegqyynqlmrealhleqVDEWLQDCFE 94
Cdd:pfam00583   3 ALYELLSEEFPEP-WPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLS--------------------IIDDEPPVGE 61

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 446596617   95 FVELAVHPQYQNEGLGTKLHNELLE-----GIpnRTSVLTTQINNEKARSLYERL 144
Cdd:pfam00583  62 IEGLAVAPEYRGKGIGTALLQALLEwarerGC--ERIFLEVAADNLAAIALYEKL 114
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
98-144 1.07e-04

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 39.12  E-value: 1.07e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 446596617  98 LAVHPQYQNEGLGTKLHNELLEGIPNR---TSVLTTQINNEKARSLYERL 144
Cdd:COG3393   21 VYTHPEYRGRGLASALVAALAREALARgarTPFLYVDADNPAARRLYERL 70
rimI PRK09491
ribosomal-protein-alanine N-acetyltransferase; Provisional
 98-144 2.61e-04

ribosomal-protein-alanine N-acetyltransferase; Provisional


Pssm-ID: 181904 [Multi-domain]  Cd Length: 146  Bit Score: 39.14  E-value: 2.61e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 446596617  98 LAVHPQYQNEGLGTKLHNELLEGIPNR---TSVLTTQINNEKARSLYERL 144
Cdd:PRK09491  69 IAVDPDYQRQGLGRALLEHLIDELEKRgvaTLWLEVRASNAAAIALYESL 118
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 91-170 2.99e-04

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 38.82  E-value: 2.99e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446596617  91 DCFEFVELAVHPQYQNEGLGTKLHNELLE-----GIPnRTSVLTTQinneKARSLYERLDWINILEPFHPSTNDVPY--V 163
Cdd:COG1246   51 DLAELRSLAVHPDYRGRGIGRRLLEALLAearelGLK-RLFLLTTS----AAIHFYEKLGFEEIDKEDLPYAKVWQRdsV 125


                 ....*..
gi 446596617 164 IMGKALK 170
Cdd:COG1246  126 VMEKDLE 132
FR47 pfam08445
FR47-like protein; The members of this family are similar to the C-terminal region of the D. ...
 98-151 3.05e-04

FR47-like protein; The members of this family are similar to the C-terminal region of the D. melanogaster hypothetical protein FR47. This protein has been found to consist of two N-acyltransferase-like domains swapped with the C-terminal strands.


Pssm-ID: 117022 [Multi-domain]  Cd Length: 86  Bit Score: 38.08  E-value: 3.05e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 446596617   98 LAVHPQYQNEGLGTKLHNELLEGIPNR--TSVLTTQINNEKARSLYERLDWINILE 151
Cdd:pfam08445  27 LQTLPEHRRRGLGSRLVAALARGIAERgiTPFAVVVAGNTPSRRLYEKLGFRKIDE 82
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 48-119 5.80e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 36.48  E-value: 5.80e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446596617  48 IVAINDENEVVGFTYGYRsmegqyynqlmrealhleqvDEWLQDCFEFVELAVHPQYQNEGLGTKLHNELLE 119
Cdd:cd04301    1 FLVAEDDGEIVGFASLSP--------------------DGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEE 52
rimI TIGR01575
ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related ...
 98-144 6.78e-04

ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related N-acetyltransferase (GNAT) superfamily. This model covers prokarotes and the archaea. The seed contains a characterized accession for Gram negative E. coli. An untraceable characterized accession (PIR|S66013) for Gram positive B. subtilis scores well (205.0) in the full alignment. Characterized members are lacking in the archaea. Noise cutoff (72.4) was set to exclude M. loti paralog of rimI. Trusted cutoff (80.0) was set at next highest scoring member in the mini-database. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 273701 [Multi-domain]  Cd Length: 131  Bit Score: 37.69  E-value: 6.78e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 446596617   98 LAVHPQYQNEGLGTKLHNEL---LEGIPNRTSVLTTQINNEKARSLYERL 144
Cdd:TIGR01575  60 IAVKPEYQGQGIGRALLRELideAKGRGVNEIFLEVRVSNIAAQALYKKL 109
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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