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Conserved domains on  [gi|446603072|ref|WP_000680418|]
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MULTISPECIES: GNAT family N-acetyltransferase [Bacillus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10003213)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 45-138 3.72e-14

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


:

Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 64.24  E-value: 3.72e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  45 EEGKIFGgvTGTMYFY---HLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSFSfQAPEFYKKHGYREYGi 121
Cdd:COG1246   35 EDGEIVG--CAALHPLdedLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTTS-AAIHFYEKLGFEEID- 110

                         90       100
                 ....*....|....*....|.
gi 446603072 122 VEDHPKGHSQH----FFEKRL 138
Cdd:COG1246  111 KEDLPYAKVWQrdsvVMEKDL 131
 
Name Accession Description Interval E-value
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 45-138 3.72e-14

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 64.24  E-value: 3.72e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  45 EEGKIFGgvTGTMYFY---HLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSFSfQAPEFYKKHGYREYGi 121
Cdd:COG1246   35 EDGEIVG--CAALHPLdedLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTTS-AAIHFYEKLGFEEID- 110

                         90       100
                 ....*....|....*....|.
gi 446603072 122 VEDHPKGHSQH----FFEKRL 138
Cdd:COG1246  111 KEDLPYAKVWQrdsvVMEKDL 131
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 40-118 3.48e-12

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 58.23  E-value: 3.48e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072   40 LVVKNEEGKI-FGGVTGTMYFYHLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSFsFQAPEFYKKHGYRE 118
Cdd:pfam13508   6 FVAEDDGKIVgFAALLPLDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETT-NRAAAFYEKLGFEE 84
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 45-101 2.30e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 47.66  E-value: 2.30e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  45 EEGKIFGGVTGTMYFY---HLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLD 101
Cdd:cd04301    6 DDGEIVGFASLSPDGSggdTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
PTZ00330 PTZ00330
acetyltransferase; Provisional
 63-117 2.69e-05

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 41.37  E-value: 2.69e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 446603072  63 HIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSfSFQAPEFYKKHGYR 117
Cdd:PTZ00330  84 HIEDVVVDPSYRGQGLGRALISDLCEIARSSGCYKVILDC-TEDMVAFYKKLGFR 137
 
Name Accession Description Interval E-value
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 45-138 3.72e-14

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 64.24  E-value: 3.72e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  45 EEGKIFGgvTGTMYFY---HLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSFSfQAPEFYKKHGYREYGi 121
Cdd:COG1246   35 EDGEIVG--CAALHPLdedLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTTS-AAIHFYEKLGFEEID- 110

                         90       100
                 ....*....|....*....|.
gi 446603072 122 VEDHPKGHSQH----FFEKRL 138
Cdd:COG1246  111 KEDLPYAKVWQrdsvVMEKDL 131
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
45-138 1.11e-13

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 63.57  E-value: 1.11e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  45 EEGKIFGGVTGTMYFYH-----LHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSFSFQAPeFYKKHGYREY 119
Cdd:COG3153   46 DDGEIVGHVALSPVDIDgegpaLLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLP-FYERFGFRPA 124

                         90
                 ....*....|....*....
gi 446603072 120 GiVEDHPKGHSQHFFEKRL 138
Cdd:COG3153  125 G-ELGLTLGPDEVFLAKEL 142
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 61-138 6.73e-13

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 60.05  E-value: 6.73e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  61 HLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLD--SFSFQAPEFYKKHGYREYGIVEDHPKGHSqHFFEKRL 138
Cdd:COG0456   13 EAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEvrEDNEAAIALYEKLGFEEVGERPNYYGDDA-LVMEKEL 91
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 40-118 3.48e-12

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 58.23  E-value: 3.48e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072   40 LVVKNEEGKI-FGGVTGTMYFYHLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSFsFQAPEFYKKHGYRE 118
Cdd:pfam13508   6 FVAEDDGKIVgFAALLPLDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETT-NRAAAFYEKLGFEE 84
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 34-116 1.08e-11

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 57.53  E-value: 1.08e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072   34 PMEKVSLVVKNEEGKIFGGVTGTM---YFYHLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSFSFQAP-- 108
Cdd:pfam00583  29 EDASEGFFVAEEDGELVGFASLSIiddEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLAai 108


                  ....*...
gi 446603072  109 EFYKKHGY 116
Cdd:pfam00583 109 ALYEKLGF 116
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 40-138 2.00e-11

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 57.37  E-value: 2.00e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  40 LVVKNEEGKIFGGVTGTMYFY-HLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSFSFQAP--EFYKKHGY 116
Cdd:COG0454   36 FIAVDDKGEPIGFAGLRRLDDkVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAaiRFYERLGF 115

                         90       100
                 ....*....|....*....|..
gi 446603072 117 REYGIVEDHPkghsQHFFEKRL 138
Cdd:COG0454  116 KEIERYVAYV----GGEFEKEL 133
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
40-138 4.70e-11

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 56.93  E-value: 4.70e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  40 LVVKNEEGKIFGGVTGTMY-----FYHLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSFSFQAP--EFYK 112
Cdd:COG1247   54 VLVAEEDGEVVGFASLGPFrprpaYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEAsiALYE 133

                         90       100       110
                 ....*....|....*....|....*....|
gi 446603072 113 KHGYREYGIVEDHPKGHSQ----HFFEKRL 138
Cdd:COG1247  134 KLGFEEVGTLPEVGFKFGRwldlVLMQKRL 163
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
63-120 3.97e-10

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 54.03  E-value: 3.97e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  63 HIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSfsfQAP--EFYKKHGYREYG 120
Cdd:COG2153   60 KIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRIVLSA---QAHavGFYEKLGFVPVG 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 45-101 2.30e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 47.66  E-value: 2.30e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  45 EEGKIFGGVTGTMYFY---HLHIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLD 101
Cdd:cd04301    6 DDGEIVGFASLSPDGSggdTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
63-120 2.84e-07

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 45.28  E-value: 2.84e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446603072  63 HIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILL--DSFSFQAPEFYKKHGYREYG 120
Cdd:COG3393   17 EISGVYTHPEYRGRGLASALVAALAREALARGARTPFLyvDADNPAARRLYERLGFRPVG 76
PTZ00330 PTZ00330
acetyltransferase; Provisional
 63-117 2.69e-05

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 41.37  E-value: 2.69e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 446603072  63 HIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSfSFQAPEFYKKHGYR 117
Cdd:PTZ00330  84 HIEDVVVDPSYRGQGLGRALISDLCEIARSSGCYKVILDC-TEDMVAFYKKLGFR 137
PLN02706 PLN02706
glucosamine 6-phosphate N-acetyltransferase
 63-121 1.98e-04

glucosamine 6-phosphate N-acetyltransferase


Pssm-ID: 178308 [Multi-domain]  Cd Length: 150  Bit Score: 38.92  E-value: 1.98e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 446603072  63 HIDFLWVDKSVRHDGYGSQLLHKIEEIAKEKGCRLILLDSFSFQAPeFYKKHGYREYGI 121
Cdd:PLN02706  87 HIEDVVVDSAARGKGLGKKIIEALTEHARSAGCYKVILDCSEENKA-FYEKCGYVRKEI 144
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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