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Conserved domains on  [gi|446614734|ref|WP_000692080|]
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MULTISPECIES: aminoglycoside phosphotransferase family protein [Bacillus]

Protein Classification

aminoglycoside phosphotransferase family protein( domain architecture ID 12025366)

aminoglycoside phosphotransferase family protein catalyzes the phosphorylation of small molecule substrates such as aminoglycoside antibiotics and N-acetylhexosamine, similar to Streptomyces vinaceus viomycin phosphotransferase

EC:  2.7.1.-
Gene Ontology:  GO:0005524|GO:0016310

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 20-244 9.31e-30

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


:

Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 112.98  E-value: 9.31e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734   20 IEEISKGFSPDKKYIITNanNEKYLFRTGDIKEY-ERKKIEFQILNEMVKRNVQ-AQRPIEIGILEEE-GVCYSIFSYLE 96
Cdd:pfam01636   2 LRPISSGASNRTYLVTTG--DGRYVLRLPPPGRAaEELRRELALLRHLAAAGVPpVPRVLAGCTDAELlGLPFLLMEYLP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734   97 GEDAKKLLPtysPKEQYDIGIEAGKDLAKMHTYEAPNNILPWYERAMKKHSKYVEAYKTCGINIKNDDKIIKFIEE-NEI 175
Cdd:pfam01636  80 GEVLARPLL---PEERGALLEALGRALARLHAVDPAALPLAGRLARLLELLRQLEAALARLLAAELLDRLEELEERlLAA 156

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446614734  176 YLKNRPN----RFQHDDFHLENIIVRDA-RYVGVVDFNGYDWGDPIHDFvkiALFARDISIPYSIGQIEGYFNG 244
Cdd:pfam01636 157 LLALLPAelppVLVHGDLHPGNLLVDPGgRVSGVIDFEDAGLGDPAYDL---AILLNSWGRELGAELLAAYLAA 227
 
Name Accession Description Interval E-value
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 20-244 9.31e-30

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 112.98  E-value: 9.31e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734   20 IEEISKGFSPDKKYIITNanNEKYLFRTGDIKEY-ERKKIEFQILNEMVKRNVQ-AQRPIEIGILEEE-GVCYSIFSYLE 96
Cdd:pfam01636   2 LRPISSGASNRTYLVTTG--DGRYVLRLPPPGRAaEELRRELALLRHLAAAGVPpVPRVLAGCTDAELlGLPFLLMEYLP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734   97 GEDAKKLLPtysPKEQYDIGIEAGKDLAKMHTYEAPNNILPWYERAMKKHSKYVEAYKTCGINIKNDDKIIKFIEE-NEI 175
Cdd:pfam01636  80 GEVLARPLL---PEERGALLEALGRALARLHAVDPAALPLAGRLARLLELLRQLEAALARLLAAELLDRLEELEERlLAA 156

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446614734  176 YLKNRPN----RFQHDDFHLENIIVRDA-RYVGVVDFNGYDWGDPIHDFvkiALFARDISIPYSIGQIEGYFNG 244
Cdd:pfam01636 157 LLALLPAelppVLVHGDLHPGNLLVDPGgRVSGVIDFEDAGLGDPAYDL---AILLNSWGRELGAELLAAYLAA 227
YcbJ COG3173
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ...
 16-251 9.15e-19

Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only];


Pssm-ID: 442406 [Multi-domain]  Cd Length: 284  Bit Score: 84.40  E-value: 9.15e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734  16 DAANIEEISKGFSpdkkyiitnanNEKYLFRTGD----------IKEYERKKIEFQILNEMVKRN-VQAQRPIEIGILEE 84
Cdd:COG3173   21 GLPEVEPLSGGWS-----------NLTYRLDTGDrlvlrrpprgLASAHDVRREARVLRALAPRLgVPVPRPLALGEDGE 89

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734  85 E-GVCYSIFSYLEGEDAKKLLPTYSPKEQYDIGIEAGKDLAKMHTYEAPNNILPWY-----ERAMKKHSKYVEAYKTCGI 158
Cdd:COG3173   90 ViGAPFYVMEWVEGETLEDALPDLSPAERRALARALGEFLAALHAVDPAAAGLADGrpeglERQLARWRAQLRRALARTD 169

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734 159 NIKN-DDKIIKFIEENEIYLknRPNRFQHDDFHLENIIVR--DARYVGVVDFNGYDWGDPIHDFVKIALFARDISIPYSI 235
Cdd:COG3173  170 DLPAlRERLAAWLAANLPEW--GPPVLVHGDLRPGNLLVDpdDGRLTAVIDWELATLGDPAADLAYLLLYWRLPDDLLGP 247

                        250       260
                 ....*....|....*....|
gi 446614734 236 GQ--IEGYFN--GRIPEEFW 251
Cdd:COG3173  248 RAafLAAYEEatGDLDDLTW 267
APH_ChoK_like cd05120
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ...
 20-222 1.52e-06

Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270690 [Multi-domain]  Cd Length: 158  Bit Score: 47.30  E-value: 1.52e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734  20 IEEISKGFSpDKKYIITNanNEKYLFRTGDIKEYERKKIE---FQILNEmvKRNVQAQRPIEIGilEEEGVCYSIFSYLE 96
Cdd:cd05120    3 VKLIKEGGD-NKVYLLGD--PREYVLKIGPPRLKKDLEKEaamLQLLAG--KLSLPVPKVYGFG--ESDGWEYLLMERIE 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734  97 GEDAKKLLPTYSPKEQYDIGIEAGKDLAKMHTyeapnniLPWYEramkkhskyveayktcginiknddkiikfieeneiy 176
Cdd:cd05120   76 GETLSEVWPRLSEEEKEKIADQLAEILAALHR-------IDSSV------------------------------------ 112

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 446614734 177 lknrpnrFQHDDFHLENIIVRD-ARYVGVVDFNGYDWGDPIHDFVKI 222
Cdd:cd05120  113 -------LTHGDLHPGNILVKPdGKLSGIIDWEFAGYGPPAFDYAAA 152
 
Name Accession Description Interval E-value
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 20-244 9.31e-30

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 112.98  E-value: 9.31e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734   20 IEEISKGFSPDKKYIITNanNEKYLFRTGDIKEY-ERKKIEFQILNEMVKRNVQ-AQRPIEIGILEEE-GVCYSIFSYLE 96
Cdd:pfam01636   2 LRPISSGASNRTYLVTTG--DGRYVLRLPPPGRAaEELRRELALLRHLAAAGVPpVPRVLAGCTDAELlGLPFLLMEYLP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734   97 GEDAKKLLPtysPKEQYDIGIEAGKDLAKMHTYEAPNNILPWYERAMKKHSKYVEAYKTCGINIKNDDKIIKFIEE-NEI 175
Cdd:pfam01636  80 GEVLARPLL---PEERGALLEALGRALARLHAVDPAALPLAGRLARLLELLRQLEAALARLLAAELLDRLEELEERlLAA 156

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446614734  176 YLKNRPN----RFQHDDFHLENIIVRDA-RYVGVVDFNGYDWGDPIHDFvkiALFARDISIPYSIGQIEGYFNG 244
Cdd:pfam01636 157 LLALLPAelppVLVHGDLHPGNLLVDPGgRVSGVIDFEDAGLGDPAYDL---AILLNSWGRELGAELLAAYLAA 227
YcbJ COG3173
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ...
 16-251 9.15e-19

Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only];


Pssm-ID: 442406 [Multi-domain]  Cd Length: 284  Bit Score: 84.40  E-value: 9.15e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734  16 DAANIEEISKGFSpdkkyiitnanNEKYLFRTGD----------IKEYERKKIEFQILNEMVKRN-VQAQRPIEIGILEE 84
Cdd:COG3173   21 GLPEVEPLSGGWS-----------NLTYRLDTGDrlvlrrpprgLASAHDVRREARVLRALAPRLgVPVPRPLALGEDGE 89

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734  85 E-GVCYSIFSYLEGEDAKKLLPTYSPKEQYDIGIEAGKDLAKMHTYEAPNNILPWY-----ERAMKKHSKYVEAYKTCGI 158
Cdd:COG3173   90 ViGAPFYVMEWVEGETLEDALPDLSPAERRALARALGEFLAALHAVDPAAAGLADGrpeglERQLARWRAQLRRALARTD 169

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734 159 NIKN-DDKIIKFIEENEIYLknRPNRFQHDDFHLENIIVR--DARYVGVVDFNGYDWGDPIHDFVKIALFARDISIPYSI 235
Cdd:COG3173  170 DLPAlRERLAAWLAANLPEW--GPPVLVHGDLRPGNLLVDpdDGRLTAVIDWELATLGDPAADLAYLLLYWRLPDDLLGP 247

                        250       260
                 ....*....|....*....|
gi 446614734 236 GQ--IEGYFN--GRIPEEFW 251
Cdd:COG3173  248 RAafLAAYEEatGDLDDLTW 267
APH_ChoK_like cd05120
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ...
 20-222 1.52e-06

Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270690 [Multi-domain]  Cd Length: 158  Bit Score: 47.30  E-value: 1.52e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734  20 IEEISKGFSpDKKYIITNanNEKYLFRTGDIKEYERKKIE---FQILNEmvKRNVQAQRPIEIGilEEEGVCYSIFSYLE 96
Cdd:cd05120    3 VKLIKEGGD-NKVYLLGD--PREYVLKIGPPRLKKDLEKEaamLQLLAG--KLSLPVPKVYGFG--ESDGWEYLLMERIE 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734  97 GEDAKKLLPTYSPKEQYDIGIEAGKDLAKMHTyeapnniLPWYEramkkhskyveayktcginiknddkiikfieeneiy 176
Cdd:cd05120   76 GETLSEVWPRLSEEEKEKIADQLAEILAALHR-------IDSSV------------------------------------ 112

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 446614734 177 lknrpnrFQHDDFHLENIIVRD-ARYVGVVDFNGYDWGDPIHDFVKI 222
Cdd:cd05120  113 -------LTHGDLHPGNILVKPdGKLSGIIDWEFAGYGPPAFDYAAA 152
SrkA COG2334
Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal ...
 33-226 3.48e-06

Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal transduction mechanisms]; Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist is part of the Pathway/BioSystem: Threonine biosynthesis


Pssm-ID: 441905 [Multi-domain]  Cd Length: 297  Bit Score: 47.61  E-value: 3.48e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734  33 YIITNANNEKY---LFRTGdikEYERKKIEFQI--LNEMVKRNVQAQRPIEI----GILEEEGVCYSIFSYLEGEDAKKL 103
Cdd:COG2334   29 YRVETEDGRRYvlkLYRPG---RWSPEEIPFELalLAHLAAAGLPVPAPVPTrdgeTLLELEGRPAALFPFLPGRSPEEP 105

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734 104 LPtyspkEQYdigIEAGKDLAKMH----TYEAPNN-ILPWY----ERAMKKHSKYVEAYKTCginikndDKIIKFIEEN- 173
Cdd:COG2334  106 SP-----EQL---EELGRLLARLHralaDFPRPNArDLAWWdellERLLGPLLPDPEDRALL-------EELLDRLEARl 170

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 446614734 174 EIYLKNRPNRFQHDDFHLENIIVRDARYVGVVDFngYD--WGDPIHDFVkIALFA 226
Cdd:COG2334  171 APLLGALPRGVIHGDLHPDNVLFDGDGVSGLIDF--DDagYGPRLYDLA-IALNG 222
CotS COG0510
Thiamine kinase or a related kinase [Coenzyme transport and metabolism];
186-284 1.93e-03

Thiamine kinase or a related kinase [Coenzyme transport and metabolism];


Pssm-ID: 440276 [Multi-domain]  Cd Length: 156  Bit Score: 38.22  E-value: 1.93e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446614734 186 HDDFHLENIIVRDARYVGVVDFNGYDWGDPIHDfvkIALFARDISIPYSIGQ--IEGYFNGRIPEEFWNLYAVYVGMTVF 263
Cdd:COG0510   53 HGDLHPGNFLVTDDGRLYLIDWEYAGLGDPAFD---LAALLVEYGLSPEQAEelLEAYGFGRPTEELLRRLRAYRALADL 129

                         90       100
                 ....*....|....*....|.
gi 446614734 264 SSVVWTLLAAPHMLDDMLERL 284
Cdd:COG0510  130 LWALWALVRAAQEANGDLLKY 150
APH_ChoK_like_1 cd05155
Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and ...
 186-215 7.13e-03

Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and Choline kinase; This subfamily is composed of uncharacterized bacterial proteins with similarity to APH and ChoK. Other APH/ChoK-like proteins include ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). These proteins catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates, such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides, and macrolides leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK-like subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270704 [Multi-domain]  Cd Length: 234  Bit Score: 37.21  E-value: 7.13e-03
                         10        20        30
                 ....*....|....*....|....*....|
gi 446614734 186 HDDFHLENIIVRDARYVGVVDFNGYDWGDP 215
Cdd:cd05155  167 HGDLHPGNLLVRDGRLSAVIDFGDLGVGDP 196
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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