NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|446628582|ref|WP_000705928|]
View 

MULTISPECIES: PTS sugar transporter subunit IIB [Enterobacteriaceae]

Protein Classification

PTS sugar transporter subunit IIB( domain architecture ID 10144912)

subunit IIB of enzyme II (EII) of the L-ascorbate-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS)

CATH:  3.40.50.2300
EC:  2.7.1.-
Gene Ontology:  GO:0008643|GO:0009401|GO:0008982
PubMed:  8246840|2197982|11361063

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
PTS_IIB_ascorbate cd05563
PTS_IIB_ascorbate: subunit IIB of enzyme II (EII) of the L-ascorbate-specific ...
 2-89 5.55e-30

PTS_IIB_ascorbate: subunit IIB of enzyme II (EII) of the L-ascorbate-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is an L-ascorbate-specific permease with two cytoplasmic subunits (IIA and IIB) and a transmembrane channel IIC subunit. Subunits IIA, IIB, and IIC are encoded by the sgaA, sgaB, and sgaT genes of the E. coli sgaTBA operon. In some bacteria, the IIB (SgaB) domain is fused C-terminal to the IIA (SgaT) domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include ascorbate, chitobiose/lichenan, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


:

Pssm-ID: 99905  Cd Length: 86  Bit Score: 101.83  E-value: 5.55e-30
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582  2 KITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKGTPSDIYVGTKDIAEQLAaqSVAGKIVALENMIDKKAM 81
Cdd:cd05563   1 KILAVCGSGLGSSLMLKMNVEKVLKELGIEAEVEHTDLGSAKASSADIIVTSKDLASLLA--DGGAKVIGLKNIMDKNEI 78


                ....*...
gi 446628582 82 RERLSVVL 89
Cdd:cd05563  79 KEKLLEAL 86
 
Name Accession Description Interval E-value
PTS_IIB_ascorbate cd05563
PTS_IIB_ascorbate: subunit IIB of enzyme II (EII) of the L-ascorbate-specific ...
 2-89 5.55e-30

PTS_IIB_ascorbate: subunit IIB of enzyme II (EII) of the L-ascorbate-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is an L-ascorbate-specific permease with two cytoplasmic subunits (IIA and IIB) and a transmembrane channel IIC subunit. Subunits IIA, IIB, and IIC are encoded by the sgaA, sgaB, and sgaT genes of the E. coli sgaTBA operon. In some bacteria, the IIB (SgaB) domain is fused C-terminal to the IIA (SgaT) domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include ascorbate, chitobiose/lichenan, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99905  Cd Length: 86  Bit Score: 101.83  E-value: 5.55e-30
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582  2 KITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKGTPSDIYVGTKDIAEQLAaqSVAGKIVALENMIDKKAM 81
Cdd:cd05563   1 KILAVCGSGLGSSLMLKMNVEKVLKELGIEAEVEHTDLGSAKASSADIIVTSKDLASLLA--DGGAKVIGLKNIMDKNEI 78


                ....*...
gi 446628582 82 RERLSVVL 89
Cdd:cd05563  79 KEKLLEAL 86
SgaB COG3414
Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and ...
 1-93 4.12e-27

Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and metabolism];


Pssm-ID: 442640  Cd Length: 94  Bit Score: 94.85  E-value: 4.12e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582  1 MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKGTPS--DIYVGTKDIAEQLAAQSVagKIVALENMIDK 78
Cdd:COG3414   2 KKILVVCGSGLGTSTMLKMKIEKVLKELGIDAEVEHCDVSSAKSKADdaDLIVTTTDLAEELGIPVI--NVIPLLNGIDK 79

                        90
                ....*....|....*
gi 446628582 79 KAMRERLSVVLTELG 93
Cdd:COG3414  80 DEIKEKLLEALKELG 94
PRK09548 PRK09548
PTS ascorbate-specific subunit IIBC;
2-85 9.65e-11

PTS ascorbate-specific subunit IIBC;


Pssm-ID: 236559 [Multi-domain]  Cd Length: 602  Bit Score: 56.29  E-value: 9.65e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582   2 KITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKGTPS--DIYVGTKDIAEQLAAQSVAGKIVaLENMIDKK 79
Cdd:PRK09548 508 RILAVCGQGQGSSMMMKMKIKKYLDKRGIPIIMDSCAVNDYKGKLEtiDIIVCSKHLANEIEFGEGKFVLG-VQNMLNPN 586


                 ....*.
gi 446628582  80 AMRERL 85
Cdd:PRK09548 587 SFGDEL 592
PTS_IIB pfam02302
PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar ...
 2-86 5.95e-07

PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The lactose/cellobiose-specific family are one of four structurally and functionally distinct group IIB PTS system cytoplasmic enzymes. The fold of IIB cellobiose shows similar structure to mammalian tyrosine phosphatases. This family also contains the fructose specific IIB subunit.


Pssm-ID: 396744  Cd Length: 92  Bit Score: 43.48  E-value: 5.95e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582   2 KITVVCGNGLGTSLMMEMSIKSILKDLS----VSADVDHVDLGSAKGTPSDIYVGTKDIAEQLAAQSVAGKI--VALENM 75
Cdd:pfam02302  1 KILTACGAGMATSLMAAEALEKAAKELGiveaQGAAGVNELTAEDIADDADVVILAPDVAVEDLARFAGKPVyvIPVKDA 80

                         90
                 ....*....|.
gi 446628582  76 IDKKAMRERLS 86
Cdd:pfam02302 81 LGMKDAEEVLE 91
 
Name Accession Description Interval E-value
PTS_IIB_ascorbate cd05563
PTS_IIB_ascorbate: subunit IIB of enzyme II (EII) of the L-ascorbate-specific ...
 2-89 5.55e-30

PTS_IIB_ascorbate: subunit IIB of enzyme II (EII) of the L-ascorbate-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is an L-ascorbate-specific permease with two cytoplasmic subunits (IIA and IIB) and a transmembrane channel IIC subunit. Subunits IIA, IIB, and IIC are encoded by the sgaA, sgaB, and sgaT genes of the E. coli sgaTBA operon. In some bacteria, the IIB (SgaB) domain is fused C-terminal to the IIA (SgaT) domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include ascorbate, chitobiose/lichenan, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99905  Cd Length: 86  Bit Score: 101.83  E-value: 5.55e-30
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582  2 KITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKGTPSDIYVGTKDIAEQLAaqSVAGKIVALENMIDKKAM 81
Cdd:cd05563   1 KILAVCGSGLGSSLMLKMNVEKVLKELGIEAEVEHTDLGSAKASSADIIVTSKDLASLLA--DGGAKVIGLKNIMDKNEI 78


                ....*...
gi 446628582 82 RERLSVVL 89
Cdd:cd05563  79 KEKLLEAL 86
SgaB COG3414
Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and ...
 1-93 4.12e-27

Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and metabolism];


Pssm-ID: 442640  Cd Length: 94  Bit Score: 94.85  E-value: 4.12e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582  1 MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKGTPS--DIYVGTKDIAEQLAAQSVagKIVALENMIDK 78
Cdd:COG3414   2 KKILVVCGSGLGTSTMLKMKIEKVLKELGIDAEVEHCDVSSAKSKADdaDLIVTTTDLAEELGIPVI--NVIPLLNGIDK 79

                        90
                ....*....|....*
gi 446628582 79 KAMRERLSVVLTELG 93
Cdd:COG3414  80 DEIKEKLLEALKELG 94
PRK09548 PRK09548
PTS ascorbate-specific subunit IIBC;
2-85 9.65e-11

PTS ascorbate-specific subunit IIBC;


Pssm-ID: 236559 [Multi-domain]  Cd Length: 602  Bit Score: 56.29  E-value: 9.65e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582   2 KITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKGTPS--DIYVGTKDIAEQLAAQSVAGKIVaLENMIDKK 79
Cdd:PRK09548 508 RILAVCGQGQGSSMMMKMKIKKYLDKRGIPIIMDSCAVNDYKGKLEtiDIIVCSKHLANEIEFGEGKFVLG-VQNMLNPN 586


                 ....*.
gi 446628582  80 AMRERL 85
Cdd:PRK09548 587 SFGDEL 592
PTS_IIB cd00133
PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the ...
 2-85 1.58e-10

PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In the multienzyme PTS complex, EII is a carbohydrate-specific permease consisting of two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system. The PTS is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, polyols, amino sugars, and other sugar derivatives. The four proteins (domains) forming the PTS phosphorylation cascade (EI, HPr, EIIA, and EIIB), can phosphorylate or interact with numerous non-PTS proteins thereby regulating their activity.


Pssm-ID: 99904  Cd Length: 84  Bit Score: 52.26  E-value: 1.58e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582  2 KITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSA-KGTPSDIYVGTKDIAEQLAAQSVAgKIVALENMIDKKA 80
Cdd:cd00133   1 KILVVCGSGIGSSSMLAEKLEKAAKELGIEVKVEAQGLSEViDLADADLIISTVPLAARFLGKPVI-VVSPLLNEKDGEK 79


                ....*
gi 446628582 81 MRERL 85
Cdd:cd00133  80 ILEKL 84
PTS_IIB pfam02302
PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar ...
 2-86 5.95e-07

PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The lactose/cellobiose-specific family are one of four structurally and functionally distinct group IIB PTS system cytoplasmic enzymes. The fold of IIB cellobiose shows similar structure to mammalian tyrosine phosphatases. This family also contains the fructose specific IIB subunit.


Pssm-ID: 396744  Cd Length: 92  Bit Score: 43.48  E-value: 5.95e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582   2 KITVVCGNGLGTSLMMEMSIKSILKDLS----VSADVDHVDLGSAKGTPSDIYVGTKDIAEQLAAQSVAGKI--VALENM 75
Cdd:pfam02302  1 KILTACGAGMATSLMAAEALEKAAKELGiveaQGAAGVNELTAEDIADDADVVILAPDVAVEDLARFAGKPVyvIPVKDA 80

                         90
                 ....*....|.
gi 446628582  76 IDKKAMRERLS 86
Cdd:pfam02302 81 LGMKDAEEVLE 91
PTS_IIB_galactitol cd05566
PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific ...
 2-53 3.26e-04

PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a galactitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain that are expressed on three distinct polypeptide chains, in contrast to other PTS sugar transporters. The three genes encoding these subunits (gatA, gatB, and gatC) comprise the gatCBA operon. Galactitol PTS permease takes up exogenous galactitol, releasing the phosphate ester into the cytoplasm in preparation for oxidation and further metabolism via a modified glycolytic pathway called the tagatose-6-phosphate glycolytic pathway. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include galactitol, chitobiose/lichenan, ascorbate, lactose, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99908  Cd Length: 89  Bit Score: 36.36  E-value: 3.26e-04
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 446628582  2 KITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKGTPS--DIYVGT 53
Cdd:cd05566   2 KILVACGTGVATSTVVASKVKELLKENGIDVKVEQCKIAEVPSLLDdaDLIVST 55
PTS_IIB_bgl_like cd05568
PTS_IIB_bgl_like: the PTS (phosphotransferase system) IIB domain of a family of sensory ...
 2-39 8.15e-04

PTS_IIB_bgl_like: the PTS (phosphotransferase system) IIB domain of a family of sensory systems composed of a membrane-bound sugar-sensor (similar to BglF) and a transcription antiterminator (similar to BglG) which regulate expression of genes involved in sugar utilization. The domain architecture of the IIB-containing protein includes a region N-terminal to the IIB domain which is homologous to the BglG transcription antiterminator with an RNA-binding domain followed by two homologous domains, PRD1 and PRD2 (PTS Regulation Domains). C-terminal to the IIB domain is a domain similar to the PTS IIA domain. In this system, the BglG-like region and the IIB and IIA-like domains are all expressed together as a single multidomain protein. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include this sensory system with similarity to the bacterial bgl system, chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, and fructose systems.


Pssm-ID: 99910  Cd Length: 85  Bit Score: 35.17  E-value: 8.15e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*...
gi 446628582  2 KITVVCGNGLGTSLMMEMSIKSILKDLSV----------SADVDHVDL 39
Cdd:cd05568   2 KALVVCPSGIGTSRLLKSKLKKLFPEIEIidvislreleEVDLDDYDL 49
PTS_IIB_mannitol cd05567
PTS_IIB_mannitol: subunit IIB of enzyme II (EII) of the mannitol-specific phosphoenolpyruvate: ...
 1-79 1.94e-03

PTS_IIB_mannitol: subunit IIB of enzyme II (EII) of the mannitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a mannitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIA, IIB, and IIC domains are expressed from the mtlA gene as a single protein, also known as the mannitol PTS permease, the mtl transporter, or MtlA. MtlA is only functional as a dimer with the dimer contacts occuring between the IIC domains. MtlA takes up exogenous mannitol releasing the phosphate ester into the cytoplasm in preparation for oxidation to fructose-6-phosphate by the NAD-dependent mannitol-P dehydrogenase (MtlD). The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include mannitol, chitobiose/lichenan, ascorbate, lactose, galactitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99909 [Multi-domain]  Cd Length: 87  Bit Score: 34.08  E-value: 1.94e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446628582  1 MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHV---DLGSakgtPSDIYVGTKDIAEQLAAQSVAGKIVALENMID 77
Cdd:cd05567   1 KKIVFACDAGMGSSAMGASVLRKKLKKAGLEIPVTNSaidELPS----DADLVVTHASLTDRAKKKAPQAQHLSVDNFLN 76


                ..
gi 446628582 78 KK 79
Cdd:cd05567  77 TP 78
BglG COG3711
Transcriptional antiterminator [Transcription];
2-30 6.30e-03

Transcriptional antiterminator [Transcription];


Pssm-ID: 442925 [Multi-domain]  Cd Length: 618  Bit Score: 34.06  E-value: 6.30e-03
                         10        20
                 ....*....|....*....|....*....
gi 446628582   2 KITVVCGNGLGTSLMMEMSIKSILKDLSV 30
Cdd:COG3711  393 RVLVVCSSGIGTSRLLKSRLKKLFPEIEI 421
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH