|
Name |
Accession |
Description |
Interval |
E-value |
| PRK07726 |
PRK07726 |
DNA topoisomerase 3; |
1-655 |
0e+00 |
|
DNA topoisomerase 3;
Pssm-ID: 236078 [Multi-domain] Cd Length: 658 Bit Score: 657.62 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGgNFTRHDGWFESDNTIVTNCFGHIIESQPPENYNPEYKEWKIETLPLRLYPVKYQPVESA 80
Cdd:PRK07726 1 MRLFIAEKPSVGRDIADVLK-PHKKGDGYIEGNGYIVTWAIGHLLELAEPEAYDERYKRWRLEDLPIIPEKWKLVVKKKT 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 81 AKQVKTIIELIRraDVTEIIHAGDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKAL 160
Cdd:PRK07726 80 AKQFNVVKKLLK--QATEIVIATDADREGELIAREILDYCGVRKPIKRLWISSLTDKAIKRAFANLKPGKETIPLYYSAL 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 161 ARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADvIRANWKPGE 240
Cdd:PRK07726 158 ARSRADWLVGINMTRAYTLLGRKAGYNGVLSVGRVQTPTLALVVRRDEEIENFVPKPYWEVEAHLTPGER-FTAKWQPSE 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 241 FAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAIT 320
Cdd:PRK07726 237 PYQDEEGRLLDRPLAEQVVARIQGQPAKVTEVETKRKKEYAPLLYDLSELQIDANKRFGLSAKETLDIAQSLYETHKLIT 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 321 YNRSDCSYLSDEQFSEAPQIIDALKSV---FDQPMDI-DTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERNVYLA 396
Cdd:PRK07726 317 YPRTDSRYLPEDMVATLPEVLNAISKVdpyLLLAPPVlDPSIRSRAWNDKKVTAHHAIIPTEQPPNLSKLSEDERKVYDL 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 397 IAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGvenagddeaEGENDDSAFDLLCKIRTGETVTTKE 476
Cdd:PRK07726 397 IARRYLAQFLPPAEYDKTTIELEIAGGTFIAKGKQVVEAGWKALLG---------KKEEDEEKEQPLPVLAKGDELKVEK 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 477 VVVNEKKTTPPPLFTEATLLAALVRVADFVTDPVIkkllkdkdRDKKDEHGGIGTPATRASILETLKKRNYITLEKGKLI 556
Cdd:PRK07726 468 GEVKEGQTQPPKRFTEGTLLSAMENIARFVQDKEL--------KKTLKETDGLGTEATRAGIIEKLFKRGYLEKKGKYIH 539
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 557 PTDTGYALIDALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEIKVSPSAPSGQsqrlssPC 636
Cdd:PRK07726 540 ATDKGKQLIDALPEELTSPDMTAKWEQALDQISEGQLSYQDFMQPLKQWLKQLVEQAKQSSEKYKFDNLTGP------KC 613
|
650 660
....*....|....*....|
gi 446687820 637 PSCGK-QIVIRPKSYSCTGC 655
Cdd:PRK07726 614 PDCGKpMLKVKGKNGKMLVC 633
|
|
| topB |
TIGR01056 |
DNA topoisomerase III, bacteria and conjugative plasmid; This model describes topoisomerase ... |
1-655 |
6.50e-157 |
|
DNA topoisomerase III, bacteria and conjugative plasmid; This model describes topoisomerase III from bacteria and its equivalents encoded on plasmids. The gene is designated topB if found in the bacterial chromosome, traE on conjugative plasmid RP4, etc. These enzymes are involved in the control of DNA topology. DNA topoisomerase III belongs to the type I topoisomerases, which are ATP-independent. [DNA metabolism, DNA replication, recombination, and repair]
Pssm-ID: 273419 [Multi-domain] Cd Length: 660 Bit Score: 469.32 E-value: 6.50e-157
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGGNfTRHDGWFESDNT-IVTNCFGHIIESQPPENYNPEYKEWKIETLPLRLYPVKYQPVES 79
Cdd:TIGR01056 1 MTLVLCEKPSQARDLATVLAKK-KKGNGYLEIGVGgFVTWAVGHLVELAEPEEYDEKYKNWRTYDLPLEPEDWQLVVSDK 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 80 AAKQVKTIIELIRRADVTEIIHAGDPDDEGQLLVDEVLEYAGNTK--PVKRVLINDNTLPAVKKALANPKNNRDFRGLYL 157
Cdd:TIGR01056 80 TKKQFNVIKRILKENKVDEVVIATDPDREGELIAREILDYLKVTDkvTIKRLWISSLVDSSIRKAFKNLRPKSETEGLYK 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 158 KALARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADVIRANWK 237
Cdd:TIGR01056 160 SGVARARADWLVGINLTRAFTKLGREAGNDGVLSVGRVQTPTLAMVVKRENEIKNFVGKPFYEVSATINKDEQEFTTEWQ 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 238 PGEFaplTDRKLLDKTWANGTATSLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYK 317
Cdd:TIGR01056 240 PYKD---EEERELHEFLAENVVTDLTQKPALVTDIEKERKKTSAPLFYDLSALQEDANKRFGISAKRTLDIAQKLYETHK 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 318 AITYNRSDCSYLSDEQFSEAPQIIDALK----SVFDQPMdIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERNV 393
Cdd:TIGR01056 317 LITYPRTDSRYLPEDEKEMLLEVLDALKvitpALLPIKK-RDELTNNRLWNDKKIEDHHAIIPTGNDFNLSDLSEEERNV 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 394 YLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDdeaegENDDSAFDLLCKirtGETVT 473
Cdd:TIGR01056 396 YKLIAQNYLMQFMPKEEYETTTIEIAIGKLMFEAKGKILQDNGWKALLGKQEEDE-----ETEDTTLPAFQK---GDELD 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 474 TKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTDpvikkllkDKDRDKKDEHGGIGTPATRASILETLKKRNYITLEKG 553
Cdd:TIGR01056 468 VETLELLEKQTKPPARYTEGTLLSAMTNPAAFVQD--------KGLKKTLKETKGLGTEATRADIIENLFKRGFIQKKKN 539
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 554 KLIPTDTGYALIDALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVdelyNDLIPMISNAnSAEIKVSPsapsgQSQR-- 631
Cdd:TIGR01056 540 KIYITKNGKLLCLLLPELLTKPDLTAQWEQYLNGISAGEKDDDDFI----NTINEMIKQT-INEEKKNP-----ETIQkv 609
|
650 660
....*....|....*....|....
gi 446687820 632 LSSPCPSCGKQIVIRPKSYSCTGC 655
Cdd:TIGR01056 610 AKEPVSCGGIAKCPAKDNGRLIDC 633
|
|
| TopA |
COG0550 |
DNA topoisomerase IA [Replication, recombination and repair]; |
1-610 |
1.29e-153 |
|
DNA topoisomerase IA [Replication, recombination and repair];
Pssm-ID: 440316 [Multi-domain] Cd Length: 541 Bit Score: 456.48 E-value: 1.29e-153
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGGNFtrhdgwfesdntIVTNCFGHIIE-SQPPENYNPEyKEWKietlplrlyPvKYQPVES 79
Cdd:COG0550 1 KKLVIVESPAKAKTIAKYLGKDY------------VVTASVGHLRDlPKPELDVDVE-NDFE---------P-KYEVIPG 57
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 80 AAKQVKTIIELIRRADvtEIIHAGDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNnrDFRGLYLKA 159
Cdd:COG0550 58 KKKQVKELKKLAKKAD--EVILATDPDREGEAIAWHLLELLGDKKPVKRVVFNEITKKAIKEAFKNPRD--IDMNLVDAQ 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 160 LARSVADAIYGLSMTRAYTipaktKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADVIRANWKPg 239
Cdd:COG0550 134 QARRILDRLVGYNLSPLLW-----KKVKRGLSAGRVQSPALRLIVEREREIEAFVPEEYWTIEATFKTGGGEFTAKLVK- 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 240 efapLTDRKLLDKTWANGTATSLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKyKAI 319
Cdd:COG0550 208 ----IDGKKILDEEEAEAILAALKGKTFTVTSVEKKPKKRNPPPPFTTSTLQQEASRKLGFSAKKTMSIAQRLYEV-GLI 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 320 TYNRSDCSYLSDEQFSEAPQIIdalKSVFDQPMDIDTTRKSKAFNSAkVTAHTAIIPTVSVPDVNALSTDERNVYLAIAQ 399
Cdd:COG0550 283 TYMRTDSVRLSEDAVAAAREYI---EALYGKEYLPEKPRVYKTSKNA-QEAHEAIRPTGVTRTPESLSGDEFKLYDLIWK 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 400 HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFeafLGVENAGDDEAEGENDDsafdLLCKIRTGETVTTKEVVV 479
Cdd:COG0550 359 RFLASQMPPAEYETTTVTIEVGGEEFRASGKVIKFPGF---LKVYGEGKDDEEEEEEK----RLPPLKEGEKLKLKKIEP 431
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 480 NEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASILETLKKRNYITLEKGKLIPTD 559
Cdd:COG0550 432 EQHFTKPPPRYTEASLVKAM-------------------------EELGIGRPSTYASIIETLQDRGYVEREGKRLVPTE 486
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*
gi 446687820 560 TGYALIDAL----PDIaVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMI 610
Cdd:COG0550 487 LGFIVIDLLeehfPDL-VDYEFTAKMEEKLDEIAEGKLDWKDVLEEFYKPFKELV 540
|
|
| Topoisom_bac |
pfam01131 |
DNA topoisomerase; This subfamily of topoisomerase is divided on the basis that these enzymes ... |
152-600 |
1.70e-115 |
|
DNA topoisomerase; This subfamily of topoisomerase is divided on the basis that these enzymes preferentially relax negatively supercoiled DNA, from a 5' phospho- tyrosine linkage in the enzyme-DNA covalent intermediate and has high affinity for single stranded DNA.
Pssm-ID: 426069 [Multi-domain] Cd Length: 410 Bit Score: 353.86 E-value: 1.70e-115
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 152 FRGLYLKALARSVADAIYGLSMTRAYTipaKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADV 231
Cdd:pfam01131 3 DENLVDAQLARRELDWLIGFNLSRLLT---LKLKGNKLLSAGRVQTPTLALIVEREREIENFVPEPYWTIEATLKDGGGV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 232 I-RANWKPGEFapltdrklLDKTWANGTATSLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQ 310
Cdd:pfam01131 80 FaNAKWKRGRI--------FDKEEAEAILEKLKKGTAVVTSVESKEKKKPPPLPFNTTTLQKEASRKLGFSAKKTMSIAQ 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 311 QLREKYKAITYNRSDCSYLSDEqfSEAPQIIDALKSVFDQ-----PMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNA 385
Cdd:pfam01131 152 KLYEKHGLITYPRTDSTYLSDD--ATLKAILEYLTKNYGKylpyaPRLLKKTKGKLTVGNAKVTAHEAIRPTGDIPVLPE 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 386 LSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDEaegenddsafdLLCK 465
Cdd:pfam01131 230 LSDDERKLYELIVRRFLASQMPPAVYETTTVVLEVGGEEFRASGKVILEDGWLKVYPYEKKEDEE-----------ELPE 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 466 IRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASILETLKKR 545
Cdd:pfam01131 299 LKEGEELKVTQVEVEEKKTKPPPRYTEASLIKAM-------------------------EKAGIGTPATRATIIETLLER 353
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*..
gi 446687820 546 NYITLEKGKLIPTDTGYALIDALPDIA--VNPDMTALWAEKQTLIENGEMTIEQFVD 600
Cdd:pfam01131 354 GYVERKKKSLVPTELGKALVEALEKFPdlVDPELTAELEEKLDEIAEGKLDWKDVLA 410
|
|
| TOP1Ac |
cd00186 |
DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA ... |
154-603 |
9.58e-89 |
|
DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA and then rejoin the broken phosphodiester backbone. Proposed catalytic mechanism of single stranded DNA cleavage is by phosphoryl transfer through a tyrosine nucleophile using acid/base catalysis. Tyr is activated by a nearby group (not yet identified) acting as a general base for nucleophilic attack on the 5' phosphate of the scissile bond. Arg and Lys stabilize the pentavalent transition state. Glu then acts as a proton donor for the leaving 3'-oxygen, upon cleavage of the scissile strand.
Pssm-ID: 238110 [Multi-domain] Cd Length: 381 Bit Score: 283.34 E-value: 9.58e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 154 GLYLKALARSVADAIYGLSMTRAYTipaKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMtghfqrgadvir 233
Cdd:cd00186 2 NLVNAQLARRILDRLVGFNLSRLLT---KKLRRKGVLSAGRVQSPTLGLIVEREREIKAFVPEDYWEI------------ 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 234 anwkpgefapltdrklldktwangtatslagKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLR 313
Cdd:cd00186 67 -------------------------------KEAVVVSVEKKEKKKNPPPPFTTSTLQQEASSKLGFSAKKTMQIAQKLY 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 314 EKyKAITYNRSDCSYLSDEQFSEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNA---LSTDE 390
Cdd:cd00186 116 EA-GLITYPRTDSTRLSEEAILEAREYIQAIYGKEYLYPAPLLGRRNPKRGKKEQGAHEAIRPTKVAPTPELeanLSEDE 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 391 RNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDEAEGEnddsafdllckIRTGE 470
Cdd:cd00186 195 FKLYELIWRRFLASQMADAKYEETTVTLEIGGEKFKASGKVLLEDGWLEVYPEEKDDEEEEPPP-----------LKEGD 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 471 TVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASILETLKKRNYITL 550
Cdd:cd00186 264 ELKLEEVELEEKETQPPPRYTEASLIKLM-------------------------EKRGIGRPSTYASIIETLLDRGYVEK 318
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*.
gi 446687820 551 EKGKLIPTDTGYALIDALPDIA---VNPDMTALWAEKQTLIENGEMTIEQFVDELY 603
Cdd:cd00186 319 EKKKLIPTELGFAVIELLEKHFpelVDPEFTAKLEEKLDEIAEGKKDYQEVLEEFY 374
|
|
| TOP1Ac |
smart00437 |
Bacterial DNA topoisomerase I DNA-binding domain; Bacterial DNA topoisomerase I and III, ... |
277-564 |
3.06e-59 |
|
Bacterial DNA topoisomerase I DNA-binding domain; Bacterial DNA topoisomerase I and III, Eukaryotic DNA topoisomeraes III, reverse gyrase alpha subunit
Pssm-ID: 214663 [Multi-domain] Cd Length: 259 Bit Score: 200.55 E-value: 3.06e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 277 KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKyKAITYNRSDCSYLSDEQFSEAPQIIDAL-KSVFDQPMDID 355
Cdd:smart00437 5 KKKNPPPPFTTSTLQQEASRKLGFSAKKTMQIAQKLYEK-GLITYPRTDSTRLSEEAVLEARNYIQKLyGKEYLDLAVVL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 356 TTRKSKAFNSAKVTAHTAIIPT---VSVPDVNALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKT 432
Cdd:smart00437 84 LKPRKPRKGKKAQGAHEAIRPTkpiFTPELEKELSEDEFKLYELIWRRFLASQMPDAKYEETKVTIKIGGEKFKAKGKTL 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 433 TDSGFEAFLGVENAGDDEaegenddsafdLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvik 512
Cdd:smart00437 164 LFDGWLKVYPEEKKEEEI-----------ELPTLKKGDELKVEEVEVEEKETKPPARYTEASLIKLM------------- 219
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|..
gi 446687820 513 kllkdkdrdkkdEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYAL 564
Cdd:smart00437 220 ------------EKRGIGRPSTYASIIETLLDRGYVTKKKKKLIPTELGIAV 259
|
|
| Toprim_Crpt |
pfam13342 |
C-terminal repeat of topoisomerase; |
654-715 |
7.61e-18 |
|
C-terminal repeat of topoisomerase;
Pssm-ID: 463851 [Multi-domain] Cd Length: 60 Bit Score: 77.90 E-value: 7.61e-18
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446687820 654 GCEFKIWKDFCGKSLTDKQIETLITKGVTSELKGFISqKTNKEFSAKVKLVDKatGKLGFEF 715
Cdd:pfam13342 1 GCGFSIGKTIAGKKLSEEQVKELLTKGKTGLIKGFKS-KKGKKFDAYLVLDDD--EKVEFDF 59
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PRK07726 |
PRK07726 |
DNA topoisomerase 3; |
1-655 |
0e+00 |
|
DNA topoisomerase 3;
Pssm-ID: 236078 [Multi-domain] Cd Length: 658 Bit Score: 657.62 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGgNFTRHDGWFESDNTIVTNCFGHIIESQPPENYNPEYKEWKIETLPLRLYPVKYQPVESA 80
Cdd:PRK07726 1 MRLFIAEKPSVGRDIADVLK-PHKKGDGYIEGNGYIVTWAIGHLLELAEPEAYDERYKRWRLEDLPIIPEKWKLVVKKKT 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 81 AKQVKTIIELIRraDVTEIIHAGDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKAL 160
Cdd:PRK07726 80 AKQFNVVKKLLK--QATEIVIATDADREGELIAREILDYCGVRKPIKRLWISSLTDKAIKRAFANLKPGKETIPLYYSAL 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 161 ARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADvIRANWKPGE 240
Cdd:PRK07726 158 ARSRADWLVGINMTRAYTLLGRKAGYNGVLSVGRVQTPTLALVVRRDEEIENFVPKPYWEVEAHLTPGER-FTAKWQPSE 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 241 FAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAIT 320
Cdd:PRK07726 237 PYQDEEGRLLDRPLAEQVVARIQGQPAKVTEVETKRKKEYAPLLYDLSELQIDANKRFGLSAKETLDIAQSLYETHKLIT 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 321 YNRSDCSYLSDEQFSEAPQIIDALKSV---FDQPMDI-DTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERNVYLA 396
Cdd:PRK07726 317 YPRTDSRYLPEDMVATLPEVLNAISKVdpyLLLAPPVlDPSIRSRAWNDKKVTAHHAIIPTEQPPNLSKLSEDERKVYDL 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 397 IAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGvenagddeaEGENDDSAFDLLCKIRTGETVTTKE 476
Cdd:PRK07726 397 IARRYLAQFLPPAEYDKTTIELEIAGGTFIAKGKQVVEAGWKALLG---------KKEEDEEKEQPLPVLAKGDELKVEK 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 477 VVVNEKKTTPPPLFTEATLLAALVRVADFVTDPVIkkllkdkdRDKKDEHGGIGTPATRASILETLKKRNYITLEKGKLI 556
Cdd:PRK07726 468 GEVKEGQTQPPKRFTEGTLLSAMENIARFVQDKEL--------KKTLKETDGLGTEATRAGIIEKLFKRGYLEKKGKYIH 539
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 557 PTDTGYALIDALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEIKVSPSAPSGQsqrlssPC 636
Cdd:PRK07726 540 ATDKGKQLIDALPEELTSPDMTAKWEQALDQISEGQLSYQDFMQPLKQWLKQLVEQAKQSSEKYKFDNLTGP------KC 613
|
650 660
....*....|....*....|
gi 446687820 637 PSCGK-QIVIRPKSYSCTGC 655
Cdd:PRK07726 614 PDCGKpMLKVKGKNGKMLVC 633
|
|
| topB |
TIGR01056 |
DNA topoisomerase III, bacteria and conjugative plasmid; This model describes topoisomerase ... |
1-655 |
6.50e-157 |
|
DNA topoisomerase III, bacteria and conjugative plasmid; This model describes topoisomerase III from bacteria and its equivalents encoded on plasmids. The gene is designated topB if found in the bacterial chromosome, traE on conjugative plasmid RP4, etc. These enzymes are involved in the control of DNA topology. DNA topoisomerase III belongs to the type I topoisomerases, which are ATP-independent. [DNA metabolism, DNA replication, recombination, and repair]
Pssm-ID: 273419 [Multi-domain] Cd Length: 660 Bit Score: 469.32 E-value: 6.50e-157
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGGNfTRHDGWFESDNT-IVTNCFGHIIESQPPENYNPEYKEWKIETLPLRLYPVKYQPVES 79
Cdd:TIGR01056 1 MTLVLCEKPSQARDLATVLAKK-KKGNGYLEIGVGgFVTWAVGHLVELAEPEEYDEKYKNWRTYDLPLEPEDWQLVVSDK 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 80 AAKQVKTIIELIRRADVTEIIHAGDPDDEGQLLVDEVLEYAGNTK--PVKRVLINDNTLPAVKKALANPKNNRDFRGLYL 157
Cdd:TIGR01056 80 TKKQFNVIKRILKENKVDEVVIATDPDREGELIAREILDYLKVTDkvTIKRLWISSLVDSSIRKAFKNLRPKSETEGLYK 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 158 KALARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADVIRANWK 237
Cdd:TIGR01056 160 SGVARARADWLVGINLTRAFTKLGREAGNDGVLSVGRVQTPTLAMVVKRENEIKNFVGKPFYEVSATINKDEQEFTTEWQ 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 238 PGEFaplTDRKLLDKTWANGTATSLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYK 317
Cdd:TIGR01056 240 PYKD---EEERELHEFLAENVVTDLTQKPALVTDIEKERKKTSAPLFYDLSALQEDANKRFGISAKRTLDIAQKLYETHK 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 318 AITYNRSDCSYLSDEQFSEAPQIIDALK----SVFDQPMdIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERNV 393
Cdd:TIGR01056 317 LITYPRTDSRYLPEDEKEMLLEVLDALKvitpALLPIKK-RDELTNNRLWNDKKIEDHHAIIPTGNDFNLSDLSEEERNV 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 394 YLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDdeaegENDDSAFDLLCKirtGETVT 473
Cdd:TIGR01056 396 YKLIAQNYLMQFMPKEEYETTTIEIAIGKLMFEAKGKILQDNGWKALLGKQEEDE-----ETEDTTLPAFQK---GDELD 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 474 TKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTDpvikkllkDKDRDKKDEHGGIGTPATRASILETLKKRNYITLEKG 553
Cdd:TIGR01056 468 VETLELLEKQTKPPARYTEGTLLSAMTNPAAFVQD--------KGLKKTLKETKGLGTEATRADIIENLFKRGFIQKKKN 539
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 554 KLIPTDTGYALIDALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVdelyNDLIPMISNAnSAEIKVSPsapsgQSQR-- 631
Cdd:TIGR01056 540 KIYITKNGKLLCLLLPELLTKPDLTAQWEQYLNGISAGEKDDDDFI----NTINEMIKQT-INEEKKNP-----ETIQkv 609
|
650 660
....*....|....*....|....
gi 446687820 632 LSSPCPSCGKQIVIRPKSYSCTGC 655
Cdd:TIGR01056 610 AKEPVSCGGIAKCPAKDNGRLIDC 633
|
|
| TopA |
COG0550 |
DNA topoisomerase IA [Replication, recombination and repair]; |
1-610 |
1.29e-153 |
|
DNA topoisomerase IA [Replication, recombination and repair];
Pssm-ID: 440316 [Multi-domain] Cd Length: 541 Bit Score: 456.48 E-value: 1.29e-153
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGGNFtrhdgwfesdntIVTNCFGHIIE-SQPPENYNPEyKEWKietlplrlyPvKYQPVES 79
Cdd:COG0550 1 KKLVIVESPAKAKTIAKYLGKDY------------VVTASVGHLRDlPKPELDVDVE-NDFE---------P-KYEVIPG 57
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 80 AAKQVKTIIELIRRADvtEIIHAGDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNnrDFRGLYLKA 159
Cdd:COG0550 58 KKKQVKELKKLAKKAD--EVILATDPDREGEAIAWHLLELLGDKKPVKRVVFNEITKKAIKEAFKNPRD--IDMNLVDAQ 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 160 LARSVADAIYGLSMTRAYTipaktKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADVIRANWKPg 239
Cdd:COG0550 134 QARRILDRLVGYNLSPLLW-----KKVKRGLSAGRVQSPALRLIVEREREIEAFVPEEYWTIEATFKTGGGEFTAKLVK- 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 240 efapLTDRKLLDKTWANGTATSLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKyKAI 319
Cdd:COG0550 208 ----IDGKKILDEEEAEAILAALKGKTFTVTSVEKKPKKRNPPPPFTTSTLQQEASRKLGFSAKKTMSIAQRLYEV-GLI 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 320 TYNRSDCSYLSDEQFSEAPQIIdalKSVFDQPMDIDTTRKSKAFNSAkVTAHTAIIPTVSVPDVNALSTDERNVYLAIAQ 399
Cdd:COG0550 283 TYMRTDSVRLSEDAVAAAREYI---EALYGKEYLPEKPRVYKTSKNA-QEAHEAIRPTGVTRTPESLSGDEFKLYDLIWK 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 400 HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFeafLGVENAGDDEAEGENDDsafdLLCKIRTGETVTTKEVVV 479
Cdd:COG0550 359 RFLASQMPPAEYETTTVTIEVGGEEFRASGKVIKFPGF---LKVYGEGKDDEEEEEEK----RLPPLKEGEKLKLKKIEP 431
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 480 NEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASILETLKKRNYITLEKGKLIPTD 559
Cdd:COG0550 432 EQHFTKPPPRYTEASLVKAM-------------------------EELGIGRPSTYASIIETLQDRGYVEREGKRLVPTE 486
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*
gi 446687820 560 TGYALIDAL----PDIaVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMI 610
Cdd:COG0550 487 LGFIVIDLLeehfPDL-VDYEFTAKMEEKLDEIAEGKLDWKDVLEEFYKPFKELV 540
|
|
| PRK08173 |
PRK08173 |
DNA topoisomerase III; Validated |
2-717 |
7.86e-126 |
|
DNA topoisomerase III; Validated
Pssm-ID: 236172 [Multi-domain] Cd Length: 862 Bit Score: 395.19 E-value: 7.86e-126
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 2 KLFIAEKPAVANDIVKALGGnFTRHDGWFESDNTIVTNCFGHIIESQPPENYNPEYKEWKIETLPLrlYPVKY--QPVES 79
Cdd:PRK08173 4 ALIIAEKPSVANDIARALGG-FTKHDEYFESDEYVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPV--IPPHFdlNPIAK 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 80 AAKQVKTIIELIRRADVTEIIHAGDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKA 159
Cdd:PRK08173 81 TESRLKVLTKLIKRKDVTRLINACDAGREGELIFRLIAQHAKAKKPVKRLWLQSMTPQAIRDGFANLRSDEDMQPLADAA 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 160 LARSVADAIYGLSMTRAYT-IPAKTKGYKgVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADVIRANW-- 236
Cdd:PRK08173 161 RCRSEADWLVGINGTRAMTaFNSKGGGFF-LTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVRAEFVAAAGFYEGRWfd 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 237 ---KPGEFAP-LTDRKLLDKTWANGTATSLAGKPATVeaaaTDDKKTA---APLPFNLVRLQQYMNKKFKMTAQKTLDIT 309
Cdd:PRK08173 240 pkfKKDEFDPeKRASRLWSEAAAEAIVAACRGKPGTV----TEESKPStqlSPLLFDLTSLQREANGRFGFSAKNTLGLA 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 310 QQLREKYKAITYNRSDCSYLSDEQFSEAPQIIDALKSVF------DQPMDIDTTRKSK-AFNSAKVTAHTAIIPTVSVPd 382
Cdd:PRK08173 316 QALYEKHKVLTYPRTDSRALPEDYLGTVKQTLEMLKESNnylphaKQILDKGWVKPNKrIFDNSKISDHFAIIPTLQAP- 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 383 vNALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDEAegenddsafdl 462
Cdd:PRK08173 395 -KSLSEPEQKLYDLVVKRFLAVFFPAAEFLVTTRITEVAGHHFKTEGKVLVNPGWLAVYGKEAQGADAN----------- 462
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 463 LCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTDPVIKKLLKDKdrdkkdehgGIGTPATRASILETL 542
Cdd:PRK08173 463 LVPVQKGEKVKTDKIEAVALTTKPPARYNEATLLSAMEGAGKLVEDDELREAMAEK---------GLGTPATRAAIIEGL 533
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 543 KKRNYITLEKGKLIPTDTGYALIDALPDIAVN----PDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEI 618
Cdd:PRK08173 534 LGEKYLVREGRELIPTAKAFQLMTLLRGLGVEeltsPELTGEWEYKLSQIERGKLSRDAFMQEIAQMTQQIVKRAKEYDS 613
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 619 KVSPsapsGQSQRLSSPCPSCGKQIVIRPKSYSCTGCEFKIWKDFCGKSLTDKQIETLITKGVTSELKGFISqKTNKEFS 698
Cdd:PRK08173 614 DTIP----GDYATLQTPCPNCGGVVKENYRRFACTKCDFSISKIPGGRQFEIAEVEELLRKKEIGPLSGFRS-KMGRPFS 688
|
730 740
....*....|....*....|
gi 446687820 699 AKVKLVDKATG-KLGFEFTQ 717
Cdd:PRK08173 689 AILKLSDETKNyKLEFDFGQ 708
|
|
| Topoisom_bac |
pfam01131 |
DNA topoisomerase; This subfamily of topoisomerase is divided on the basis that these enzymes ... |
152-600 |
1.70e-115 |
|
DNA topoisomerase; This subfamily of topoisomerase is divided on the basis that these enzymes preferentially relax negatively supercoiled DNA, from a 5' phospho- tyrosine linkage in the enzyme-DNA covalent intermediate and has high affinity for single stranded DNA.
Pssm-ID: 426069 [Multi-domain] Cd Length: 410 Bit Score: 353.86 E-value: 1.70e-115
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 152 FRGLYLKALARSVADAIYGLSMTRAYTipaKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADV 231
Cdd:pfam01131 3 DENLVDAQLARRELDWLIGFNLSRLLT---LKLKGNKLLSAGRVQTPTLALIVEREREIENFVPEPYWTIEATLKDGGGV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 232 I-RANWKPGEFapltdrklLDKTWANGTATSLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQ 310
Cdd:pfam01131 80 FaNAKWKRGRI--------FDKEEAEAILEKLKKGTAVVTSVESKEKKKPPPLPFNTTTLQKEASRKLGFSAKKTMSIAQ 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 311 QLREKYKAITYNRSDCSYLSDEqfSEAPQIIDALKSVFDQ-----PMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNA 385
Cdd:pfam01131 152 KLYEKHGLITYPRTDSTYLSDD--ATLKAILEYLTKNYGKylpyaPRLLKKTKGKLTVGNAKVTAHEAIRPTGDIPVLPE 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 386 LSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDEaegenddsafdLLCK 465
Cdd:pfam01131 230 LSDDERKLYELIVRRFLASQMPPAVYETTTVVLEVGGEEFRASGKVILEDGWLKVYPYEKKEDEE-----------ELPE 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 466 IRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASILETLKKR 545
Cdd:pfam01131 299 LKEGEELKVTQVEVEEKKTKPPPRYTEASLIKAM-------------------------EKAGIGTPATRATIIETLLER 353
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*..
gi 446687820 546 NYITLEKGKLIPTDTGYALIDALPDIA--VNPDMTALWAEKQTLIENGEMTIEQFVD 600
Cdd:pfam01131 354 GYVERKKKSLVPTELGKALVEALEKFPdlVDPELTAELEEKLDEIAEGKLDWKDVLA 410
|
|
| PRK14724 |
PRK14724 |
DNA topoisomerase III; Provisional |
3-715 |
1.47e-101 |
|
DNA topoisomerase III; Provisional
Pssm-ID: 237803 [Multi-domain] Cd Length: 987 Bit Score: 333.85 E-value: 1.47e-101
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 3 LFIAEKPAVANDIVKAL---GGNFTRHDGWFESDNTIVTNCFGHIIESQPPENYNPEYKEWKIETLPLRLYPVKYQPVES 79
Cdd:PRK14724 4 LVIAEKPSVAQDIVRALtpvAGKFEKHDEHFESDSYVVTSAVGHLVEIQAPEEFDVKRGKWSFANLPVIPPYFDLKPVDK 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 80 AAKQVKTIIELIRRADVTEIIHAGDPDDEGQLLVDEVLEYAGNT-----KPVKRVLINDNTLPAVKKALANPKNNRDFRG 154
Cdd:PRK14724 84 TKTRLNAVVKLAKRKDVTELVNACDAGREGELIFRLIEQYAGGAkgglgKPVKRLWLQSMTPQAIRDGFDNLRSDAQMQG 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 155 LYLKALARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADVIRA 234
Cdd:PRK14724 164 LASAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSLVVEREEKIRKFVSRDYWEIHAGFHAEAGEYLG 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 235 NWKPGEFAPLTDRKLL--DKTWANGTATSLA----GKPATVeaaaTDDKK---TAAPLPFNLVRLQQYMNKKFKMTAQKT 305
Cdd:PRK14724 244 KWFDPQWKKASDDPEAraDRVWSEREARAIAdavrGKAATV----TEESKpttQASPLLFDLTSLQREANGKFGFSAKTT 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 306 LDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQIIDALKS--------VFDQPMDIDTTRKSK-AFNSAKVTAHTAIIP 376
Cdd:PRK14724 320 LALAQSLYERHKALTYPRTDSRALPEDYLPVAKQTFEMLATsgmrhlapFAQQALDGNYVRPSKrIFDNSKVSDHFAIIP 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 377 TVSVPdvNALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDEAEGEND 456
Cdd:PRK14724 400 TTQAP--SGLSEAEQKLYDLVVRRFMAVFFPSAEYQVTTRISQVVGHSFKTEGKVLVKPGWLAIYGKEAANEVEDAKDGD 477
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 457 DSafDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTDPVIkkllkdkdrDKKDEHGGIGTPATRA 536
Cdd:PRK14724 478 KG--QPLVPVKPGEMVRTEFAEAKGLKTKPPARYSEATLLGAMESAGKQIDDDEL---------REAMQEKGLGTPATRA 546
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 537 SILETLKKRNYITLEKGKLIPTDTGYALIDALPDIAVN----PDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISN 612
Cdd:PRK14724 547 AIIEGLLTEKYMLREGRELIPTAKAFQLMTLLRGLGVEelsrAELTGEWEYKLAQMEKGQLSREAFMQQIAAMTEKLVKK 626
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 613 ANSAEIKVSPsapsGQSQRLSSPCPSCGKQIVIRPKSYSCTG-------CEFKIWKDFCGKSLTDKQIETLITKGVTSEL 685
Cdd:PRK14724 627 AKEYDRDTIP----GDYATLSTPCPNCGGVVKENYRRYACTGangagegCGFSFTKSPAGRTFETAEAEQLLRDRRIGPL 702
|
730 740 750
....*....|....*....|....*....|...
gi 446687820 686 KGFISqKTNKEFSAKVKLV-DKATG--KLGFEF 715
Cdd:PRK14724 703 EGFRS-KAGWPFTAEVVIKrDEEAKnyKLEFDF 734
|
|
| TOP1Ac |
cd00186 |
DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA ... |
154-603 |
9.58e-89 |
|
DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA and then rejoin the broken phosphodiester backbone. Proposed catalytic mechanism of single stranded DNA cleavage is by phosphoryl transfer through a tyrosine nucleophile using acid/base catalysis. Tyr is activated by a nearby group (not yet identified) acting as a general base for nucleophilic attack on the 5' phosphate of the scissile bond. Arg and Lys stabilize the pentavalent transition state. Glu then acts as a proton donor for the leaving 3'-oxygen, upon cleavage of the scissile strand.
Pssm-ID: 238110 [Multi-domain] Cd Length: 381 Bit Score: 283.34 E-value: 9.58e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 154 GLYLKALARSVADAIYGLSMTRAYTipaKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMtghfqrgadvir 233
Cdd:cd00186 2 NLVNAQLARRILDRLVGFNLSRLLT---KKLRRKGVLSAGRVQSPTLGLIVEREREIKAFVPEDYWEI------------ 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 234 anwkpgefapltdrklldktwangtatslagKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLR 313
Cdd:cd00186 67 -------------------------------KEAVVVSVEKKEKKKNPPPPFTTSTLQQEASSKLGFSAKKTMQIAQKLY 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 314 EKyKAITYNRSDCSYLSDEQFSEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNA---LSTDE 390
Cdd:cd00186 116 EA-GLITYPRTDSTRLSEEAILEAREYIQAIYGKEYLYPAPLLGRRNPKRGKKEQGAHEAIRPTKVAPTPELeanLSEDE 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 391 RNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDEAEGEnddsafdllckIRTGE 470
Cdd:cd00186 195 FKLYELIWRRFLASQMADAKYEETTVTLEIGGEKFKASGKVLLEDGWLEVYPEEKDDEEEEPPP-----------LKEGD 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 471 TVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASILETLKKRNYITL 550
Cdd:cd00186 264 ELKLEEVELEEKETQPPPRYTEASLIKLM-------------------------EKRGIGRPSTYASIIETLLDRGYVEK 318
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*.
gi 446687820 551 EKGKLIPTDTGYALIDALPDIA---VNPDMTALWAEKQTLIENGEMTIEQFVDELY 603
Cdd:cd00186 319 EKKKLIPTELGFAVIELLEKHFpelVDPEFTAKLEEKLDEIAEGKKDYQEVLEEFY 374
|
|
| PRK07219 |
PRK07219 |
DNA topoisomerase I; Validated |
1-658 |
3.93e-71 |
|
DNA topoisomerase I; Validated
Pssm-ID: 235971 [Multi-domain] Cd Length: 822 Bit Score: 247.99 E-value: 3.93e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKAL-GGNFT--RHDGW----FESDN--TIVTNCFGHIIESQPPENYNpEYKEWKIETLPlRLYP 71
Cdd:PRK07219 1 TELIIAEKNNAARRIADILsGGKAKkkRVNGVpyyeFERKGekWIVIGLSGHIVTVDFPEEYG-DWRDVDPAELI-DADP 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 72 VKYQPVESAAKQVKTiieLIRRADvtEIIHAGDPDDEGQLLVDEV---LEYAGNtKPVKRVLINDNTLPAVKKALANPkN 148
Cdd:PRK07219 79 VKKITKQNYINALKK---LAKDAD--EIIIATDYDREGELIGKEAyhiLREVCQ-VPVKRARFSSLTKKEIRKAFENP-D 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 149 NRDFrGLYLKALARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRG 228
Cdd:PRK07219 152 EIDF-NLADAGEARQIIDLYWGAALTRFLSLSVRQLGRWDFLSVGRVQTPTLAFIVDREREIRAFDPEDYWKIEALLDKE 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 229 ADVIRANWKPGEFApltdRKLLDKTWANGTATSLAG-KPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLD 307
Cdd:PRK07219 231 AQYFYRDLIGGHEA----EKFWDEEEAEEIYEKLKGaKEATVSSVKKRERTISPPAPFNTTEFLREASKIFGISPKRAME 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 308 ITQQLREK-YkaITYNRSDCSYLSD--------EQFSEAPQIIDALKSVFDQPmDIDTTRKSKafnsaKVTAHTAIIPtV 378
Cdd:PRK07219 307 IAEKLYTAgY--ISYPRTDNTVYPDdldpkellKKLSKKKEYGPYAESILEQE-NIKPTEGKK-----ETTDHPPIHP-V 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 379 SVPDVNALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDEaegendds 458
Cdd:PRK07219 378 DVPKREELSDDEWKVYELIVRRFLATLADPAEWEYLKVELDVNGEIFKASGSRLVEEGWHEVYPYEKFDEKE-------- 449
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 459 afdlLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASI 538
Cdd:PRK07219 450 ----LPDLEEGEKLKVNKIEIEAKETQPPKRYTQSSLIKEM-------------------------EKRGLGTKATRHDI 500
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 539 LETLKKRNYITlEKGKLIPTDTGYALIDAL----PDIaVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISN-- 612
Cdd:PRK07219 501 IEKLYKRGYVI-EGDPPRPTDLGIAVIEALekyaPEI-VSEEMTAQLEADMQAIEDGKKKKEDVTEESREMLKEILSElk 578
|
650 660 670 680 690
....*....|....*....|....*....|....*....|....*....|....*...
gi 446687820 613 ANSAEIKV----SPSAPSGQSQRLsSPCPSCGKQ-IVIRPKSYS----CTG---CEFK 658
Cdd:PRK07219 579 EKRKEIGDhlagSLKAENRSLKTI-GKCPECGGDlIIIRTDKGSrfvgCSGypdCRNT 635
|
|
| PRK06599 |
PRK06599 |
DNA topoisomerase I; Validated |
1-646 |
2.36e-64 |
|
DNA topoisomerase I; Validated
Pssm-ID: 235840 [Multi-domain] Cd Length: 675 Bit Score: 226.28 E-value: 2.36e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGGNFTrhdgwfesdntiVTNCFGHIIESqpPENynpeykewKIETLPLRLYPVKYQPVESA 80
Cdd:PRK06599 3 KKLVIVESPAKAKTIKKYLGKDYK------------VLASFGHVRDL--PKK--------KGGVDPDNDFAPKYEIIEGK 60
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 81 AKQVKTIIELIRRADvtEIIHAGDPDDEGQLL---VDEVLEYAGNT-KPVKRVLINDNTLPAVKKALANPKN-NRDfrgL 155
Cdd:PRK06599 61 EKVVDALKKAAKKAD--AVYLATDPDREGEAIawhIAEVLKEAKLKdKNVKRVVFNEITKKAVQEAIENPRDiDMD---L 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 156 YLKALARSVADAIYGlsmtraYTI-PAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGAD-VIR 233
Cdd:PRK06599 136 VDAQQARRALDYLVG------FKLsPLLWKKVRRGLSAGRVQSVALRLICEREDEIEAFIPQEYWTIEADLATSNGePFT 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 234 ANW------KPGEFApLTDRKLldktwANGTATSLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLD 307
Cdd:PRK06599 210 AKLvevngkKLEKFS-ITNEEQ-----AKALVKALEGQAYTVDKIEKKERKRNPPPPFITSTLQQEASRKLGFSAKKTMR 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 308 ITQQLrekYKA----------ITYNRSDCSYLSDEQFSEAPQIIdalKSVFDQPMDIDTTRKSKafNSAKVT--AHTAII 375
Cdd:PRK06599 284 IAQKL---YEGidlgegtvglITYMRTDSVRLSNEALDEARKYI---TKKYGKEYLPAKPRVYK--KKSKNAqeAHEAIR 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 376 PTvSV---PD--VNALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARArkttdSG----FEAFLGVENA 446
Cdd:PRK06599 356 PT-SInrtPEslKPYLTPDQFKLYELIWKRTVASQMAPAILDQTSVDIASENGKYVFRA-----TGsvilFPGFLKVYGE 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 447 GDDEAEGENDDsafdLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEH 526
Cdd:PRK06599 430 SKDDEEEDDEK----LLPPLKEGEKLKLDELLPEQHFTEPPPRYSEASLVKKL-------------------------EE 480
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 527 GGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYA----LIDALPDIaVNPDMTALWAEKQTLIENGEMTIEQFVDEL 602
Cdd:PRK06599 481 YGIGRPSTYASIISTLQDREYVELEKKRFIPTDLGRIvndfLVEHFPKY-VDYDFTAGLEDELDEIAEGKKDWKPVLREF 559
|
650 660 670 680
....*....|....*....|....*....|....*....|....*
gi 446687820 603 YNDLIPMISNANSAEIKVSPsapsgQSQRLSSPCPSC-GKQIVIR 646
Cdd:PRK06599 560 WEPFISNLEKVEEDVRTEKV-----TQEETDETCPKCgGGPLVLK 599
|
|
| TOP1Ac |
smart00437 |
Bacterial DNA topoisomerase I DNA-binding domain; Bacterial DNA topoisomerase I and III, ... |
277-564 |
3.06e-59 |
|
Bacterial DNA topoisomerase I DNA-binding domain; Bacterial DNA topoisomerase I and III, Eukaryotic DNA topoisomeraes III, reverse gyrase alpha subunit
Pssm-ID: 214663 [Multi-domain] Cd Length: 259 Bit Score: 200.55 E-value: 3.06e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 277 KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKyKAITYNRSDCSYLSDEQFSEAPQIIDAL-KSVFDQPMDID 355
Cdd:smart00437 5 KKKNPPPPFTTSTLQQEASRKLGFSAKKTMQIAQKLYEK-GLITYPRTDSTRLSEEAVLEARNYIQKLyGKEYLDLAVVL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 356 TTRKSKAFNSAKVTAHTAIIPT---VSVPDVNALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKT 432
Cdd:smart00437 84 LKPRKPRKGKKAQGAHEAIRPTkpiFTPELEKELSEDEFKLYELIWRRFLASQMPDAKYEETKVTIKIGGEKFKAKGKTL 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 433 TDSGFEAFLGVENAGDDEaegenddsafdLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvik 512
Cdd:smart00437 164 LFDGWLKVYPEEKKEEEI-----------ELPTLKKGDELKVEEVEVEEKETKPPARYTEASLIKLM------------- 219
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|..
gi 446687820 513 kllkdkdrdkkdEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYAL 564
Cdd:smart00437 220 ------------EKRGIGRPSTYASIIETLLDRGYVTKKKKKLIPTELGIAV 259
|
|
| TOPRIM_TopoIA_TopoIII |
cd03362 |
TOPRIM_TopoIA_TopoIII: The topoisomerase-primase (TORPIM) domain found in members of the type ... |
1-146 |
7.52e-49 |
|
TOPRIM_TopoIA_TopoIII: The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving one strand of the DNA duplex, covalently linking to the 5' phosphoryl end of the DNA break and, allowing the other strand of the duplex to pass through the gap. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). For topoisomerases the conserved glutamate is believed to act as a general base in strand joining and, as a general acid in strand cleavage. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function.
Pssm-ID: 173782 [Multi-domain] Cd Length: 151 Bit Score: 168.57 E-value: 7.52e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGGNFTR------HDGWFESDNTIVTNCFGHIIESQPPENYNPEYKEWKIETlPLRLYPVKY 74
Cdd:cd03362 1 MVLIIAEKPSVAKAIAKILGGGSKKkgkgryYEFYGEGGGYVVTWASGHLLELDFPEEYDPWDKVWPLED-PLFPAPFKL 79
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446687820 75 QPVESAAKQVKTIIELIRRADvtEIIHAGDPDDEGQLLVDEVLEYAGN--TKPVKRVLINDNTLPAVKKALANP 146
Cdd:cd03362 80 KVDKGKKKQFKVLKKLAKRAD--EIVIATDADREGELIGREILEYAKCvkRKPVKRAWFSSLTPKAIRRAFKNL 151
|
|
| PRK07561 |
PRK07561 |
DNA topoisomerase I subunit omega; Validated |
1-654 |
3.33e-48 |
|
DNA topoisomerase I subunit omega; Validated
Pssm-ID: 236048 [Multi-domain] Cd Length: 859 Bit Score: 182.69 E-value: 3.33e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGGNFtrhdgwfesdntIVTNCFGHI-----IESQPPENYNPE---------YKEWKietlp 66
Cdd:PRK07561 2 KSLVIVESPAKAKTINKYLGSDY------------VVKASVGHIrdlptSASSVPAKEKGAlwarmgvdpDHDFE----- 64
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 67 lrlypVKYQPVESAAKQVKTIIELIRRADvtEIIHAGDPDDEGQ-----LLvdEVLEyaGNTKPVKRVLINDNTLPAVKK 141
Cdd:PRK07561 65 -----ALYEVLPGKEKVVSELKKAAKDAD--ELYLATDPDREGEaiawhLL--EVLG--GDDVPVKRVVFNEITKNAIQE 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 142 ALANPknnRDFRGLYLKA-LARSVADAIYGlsmtraYTI-PAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYY 219
Cdd:PRK07561 134 AFENP---RELDINLVNAqQARRFLDRLVG------YMVsPLLWKKIARGLSAGRVQSVAVRLIVEREREIEAFVPEEYW 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 220 TMTGHFQ--RG----ADVIRANWKPgeFAPLTdrkLLDKTWANGTATSLAGKPATVeaAATDDKKT---AAPlPFNLVRL 290
Cdd:PRK07561 205 DIHADLTtpRGdafeARLTHLDGKK--FAPVD---LLNEAQAEAAVAALEGARYSV--ASVEDKPTtrkPSA-PFTTSTL 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 291 QQYMNKKFKMTAQKTLDITQQLrekYKA--ITYNRSDCSYLSDEQFSEAPQIIDAL---KSVFDQPmdidttR--KSKAF 363
Cdd:PRK07561 277 QQEASRKLGFSVKKTMRIAQRL---YEAgyITYMRTDSTNLSQEAINAARGYIGDNygkKYLPEKP------RqySSKAK 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 364 NSAKvtAHTAIIPT-VSV-PD-VNALSTDERNVYLAIAQhylvQF----MPEKAYQEVSVAIQCGDESFYARARKTTdsg 436
Cdd:PRK07561 348 NAQE--AHEAIRPSdVFRtPDqLKGLEGDAQRLYELIWK----RFvasqMAPARYDSTTVTIAAGDAELRATGRVLR--- 418
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 437 FEAFLGVENAGDDEAEGENDDsafdLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllk 516
Cdd:PRK07561 419 FDGFLKVYVEGRDDALDDEDR----RLPALKVGDALTLEKLDPTQHFTKPPARYSEASLVKEL----------------- 477
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 517 dkdrdkkdEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYALIDAL----PDIaVNPDMTALWAEKQTLIENGE 592
Cdd:PRK07561 478 --------EKLGIGRPSTYASIISTIQDRGYVRLENRRFYPEKMGRIVTDRLeeffPRL-VDYDFTARMEEELDDIANGE 548
|
650 660 670 680 690 700
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446687820 593 MTIEQFVDELYNDLipmisNANSAEIKVSPSA---PSGQSQRLSSPCPSCGKQIVIRPKSY----SCTG 654
Cdd:PRK07561 549 ADWKPVLDDFYKDF-----SQQLEKAEELREEggmRPNQMVITDRDCPTCGRGMGIRTGKTgvflGCSG 612
|
|
| PRK08780 |
PRK08780 |
DNA topoisomerase I; Provisional |
3-564 |
3.37e-48 |
|
DNA topoisomerase I; Provisional
Pssm-ID: 181555 [Multi-domain] Cd Length: 780 Bit Score: 182.27 E-value: 3.37e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 3 LFIAEKPAVANDIVKALGGNFTrhdgwfesdntiVTNCFGHIIESQPPEN-YNPEYKewkietlplrlYPVKYQPVESAA 81
Cdd:PRK08780 5 LVIVESPAKAKTINKYLGKDFT------------VLASYGHVRDLVPKEGaVDPENG-----------FAMRYDLIDKNE 61
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 82 KQVKTIIELIRRADvtEIIHAGDPDDEGQLL---VDEVLEYAG--NTKPVKRVLINDNTLPAVKKALANPknnRDFRGLY 156
Cdd:PRK08780 62 KHVEAIAKAAKSAD--DLYLATDPDREGEAIswhLAEILKERGllKDKPMQRVVFTEITPRAIKEAMAKP---RDIASDL 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 157 LKAL-ARSVADAIYGLSMTraytiPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMtghfqrGADVIRAN 235
Cdd:PRK08780 137 VDAQqARRALDYLVGFNLS-----PLLWRKIQRGLSAGRVQSPALRMIVEREEEIEAFIAREYWSI------EADCAHPS 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 236 WK-PGEFAPLTDRKLLDKTWANGTaTSLAGKPATVEAAA----------TDDKKTAAPlPFNLVRLQQYMNKKFKMTAQK 304
Cdd:PRK08780 206 QPfNAKLIKLDGQKFEQFTITDGD-TAEAARLRIQQAAQgtlhvtdvesKERKRNPAP-PFTTSTLQQEASRKLGFTTRR 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 305 TLDITQQLREKYKA--------ITYNRSDCSYLSDEQFSEAPQIID---ALKSVFDQPmdidTTRKSKAFNSAKvtAHTA 373
Cdd:PRK08780 284 TMQVAQKLYEGVDLgdegsvglITYMRTDSVNLSQDALAEIRDVIArdyGTASLPDQP----NTYKTKSKNAQE--AHEA 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 374 IIPT--VSVPDVNA--LSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDE-SFYARARKTTDSGFeafLGVENAGD 448
Cdd:PRK08780 358 VRPTsaLRTPAQVArfLSDDQRRLYELIWKRAVACQMIPATLNTVSVDLAAGSEhVFRATGSTVVVPGF---LAVYEEGK 434
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 449 DEAEGENDDSAfDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGG 528
Cdd:PRK08780 435 DDKSAEDEDEG-RKLPPMKEGDNVPLERIRAEQHFTEPPPRYTEASLVKAL-------------------------EEYG 488
|
570 580 590
....*....|....*....|....*....|....*.
gi 446687820 529 IGTPATRASILETLKKRNYITLEKGKLIPTDTGYAL 564
Cdd:PRK08780 489 IGRPSTYASIISTLQFRKYVEMEGRRFRPTDVGRAV 524
|
|
| PRK07220 |
PRK07220 |
DNA topoisomerase I; Validated |
1-646 |
3.90e-44 |
|
DNA topoisomerase I; Validated
Pssm-ID: 180892 [Multi-domain] Cd Length: 740 Bit Score: 169.94 E-value: 3.90e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGG---NFTRHDG------WFESDNTIVTNCFGHIIESQPPENYNpeykEW-KIETLPLRLY 70
Cdd:PRK07220 1 MHLIITEKNIAARRIAQILAPkkpKKTRVSGvdvyryEDNGDDTVVVGLSGHIVNIDFPKEYN----NWqKVDARDLIDA 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 71 PVKYQPVEsaAKQVKTIIELIRRADVTEIihAGDPDDEGQLLVDEVLEYAGNTKP---VKRVLINDNTLPAVKKALANPK 147
Cdd:PRK07220 77 EIITTPTQ--KKIVTALKKLGKEADRVTI--ATDYDREGELIGVEALNIIKKVNPdikFDRVRYSAITKKEIERAFSNPV 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 148 NnRDFRgLYLKALARSVADAIYGLSMTRAYTIPAKTKGyKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQR 227
Cdd:PRK07220 153 E-VDFN-LADAGHSRQVIDLVWGAALTRYISLAAGRLG-KMFLSVGRVQSPTLALIVDREKEREAFVPTPYWEIYATLEN 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 228 GADVIRANWKPGEFapltdrklLDKTWANGTATSLaGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNkKFKMTAQKTLD 307
Cdd:PRK07220 230 NGETFVAQHSTRRF--------WEKEEADRVFEKL-GKTAEVTEVEKGTKTDKPPTPFNTTEFISAAN-SIGFSAANAMR 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 308 ITQQL-REKYkaITYNRSDCSYLSD--------EQFSEAPQIIDALKsVFDQPmDIDTTRKSKafnsaKVTAHTAIIPTv 378
Cdd:PRK07220 300 IAESLyTNGY--ISYPRTDNTVYPEsldlreqiEIFAEGPFGEYAQK-LLEKG-ELVPTRGKK-----ETTDHPPIYPA- 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 379 SVPDVNALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVeNAGDDEaegendds 458
Cdd:PRK07220 370 SLAKKSELKEDEWKVYELVVRRFFATFAGPAEWETMKLRFDIGGEEFRANGSRLTEPGWRWYYPY-NAPEDR-------- 440
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 459 afdLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASI 538
Cdd:PRK07220 441 ---LLPELSEGEELKVKKKEMLDKETQPPGRYGQGRLIKLM-------------------------EDLGLGTKATRHEI 492
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 539 LETLKKRNYItlEKGKLIPTDTGYALIDALPDIA---VNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANS 615
Cdd:PRK07220 493 ISKLYSRAYI--HGNPIQPTNTAFAVVDALEKYAptiTKPDMTSRLEEDMDKIAEGKIKEDAVLEESREMLEQVFDELDK 570
|
650 660 670
....*....|....*....|....*....|..
gi 446687820 616 AEIKVSPSAPSG-QSQRLSSPCPSCGKQIVIR 646
Cdd:PRK07220 571 NREKIRESLREGlREDKIIGKCPLCGSDLMVR 602
|
|
| TOPRIM_TopoIA |
cd01028 |
TOPRIM_TopoIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the ... |
1-146 |
5.79e-44 |
|
TOPRIM_TopoIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in the type IA family of DNA topoisomerases (TopoIA). This subgroup contains proteins similar to the Type I DNA topoisomerases: E. coli topisomerases I and III, eukaryotic topoisomerase III and, ATP-dependent reverse gyrase found in archaea and thermophilic bacteria. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA. These enzymes cleave one strand of the DNA duplex, covalently link to the 5' phosphoryl end of the DNA break and allow the other strand of the duplex to pass through the gap. Reverse gyrase is also able to insert positive supercoils in the presence of ATP and negative supercoils in the presence of AMPPNP. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). For topoisomerases the conserved glutamate is believed to act as a general base in strand joining and, as a general acid in strand cleavage. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function.
Pssm-ID: 173778 [Multi-domain] Cd Length: 142 Bit Score: 154.69 E-value: 5.79e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGGNFTRHDGWFESDNTIVTNCFGHIIESQPPENYNPEYKEWKIEtlpLRLYPVKYQPVESA 80
Cdd:cd01028 1 KVLIIAEKPSKAKTIAKILGKGSKKKGFYGEGGGYVVTASVGHLLELPFPEEYVDWDKDWPLE---LFPFEPKYVVIPDK 77
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446687820 81 AKQVKTIIELIRRADvtEIIHAGDPDDEGQLLVDEVLEYAGN-TKPVKRVLINDNTLPAVKKALANP 146
Cdd:cd01028 78 KKQLKALKKLAKKAD--EIVLATDPDREGELIAWEILEVLKCdNKPVKRAWFSEITPKAIREAFKNL 142
|
|
| PRK05776 |
PRK05776 |
DNA topoisomerase I; Provisional |
3-640 |
7.62e-44 |
|
DNA topoisomerase I; Provisional
Pssm-ID: 235602 [Multi-domain] Cd Length: 670 Bit Score: 168.25 E-value: 7.62e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 3 LFIAEKPAVANDIVKALGGNFTR------------HDGwfesDNTIVTNCFGHIIESQPPENYNPEYK-EWKietlPLrl 69
Cdd:PRK05776 4 LVIAEKPKAARKIAEALSEKPIRcriygvpywivkRDG----KKIVVAPAAGHLFGLHTKSKGFPVFDyEWK----PL-- 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 70 ypvkYQpVESAAKQVKTIIELIRR--ADVTEIIHAGDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPK 147
Cdd:PRK05776 74 ----YE-IDKGSKYTKKYYELLSSlsKYADEFINACDYDIEGSVIGYLIIKYLGDPKKAKRMKFSALTKSDIRRAFRNLE 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 148 NnRDFrGLYLKALARSVADAIYGLSMTRAYTIPAKT-KGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQ 226
Cdd:PRK05776 149 T-LDY-EMINAGIARHELDWLWGINVSRALMSSVRDaSGKRVILSAGRVQSPTLKYVVEREIERNLFVPLPYFSVSIIIE 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 227 RGADVIRANWKPGEFAPLTD-RKLLDKTwangtatsLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKT 305
Cdd:PRK05776 227 KNGYEFTLKYENKKFETKEEaKEILEEI--------KKTGYLKVTKVEVKIEILEPPPPFNLGDLQVEAARIYGFSPYKT 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 306 LDITQQLREKyKAITYNRSDCSYL-SDEQFSEapqIIDALKSVFDQPMDIDTTRKS-----KAFNSAKVT-AHTAIIPTV 378
Cdd:PRK05776 299 QSIAEDLYLD-GLISYPRTNSQKLpPTLNIRN---ILKGLSRSPQYRPLVNLLLKEtkgvlKPVQGPKDDpAHPAIYPTG 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 379 SVPdvNALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDE--SFYARARKTTDSGFEAFLGVENAGDDEaegend 456
Cdd:PRK05776 375 EPP--KNLSKDEFKLYDLIVRRFLASFAAPAVLSNTIVTLRVPGFplVFSASGQRIEERGWLKYYPFHKFDEEE------ 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 457 dsafdlLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRA 536
Cdd:PRK05776 447 ------LPLLKKGERVKIVDVKVRKSYTKPPSRYSKASLLKWM-------------------------ESVGIGTEATRA 495
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 537 SILETLKKRNYITLEKGKLIPTDTGYALIDAL----PDIaVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISN 612
Cdd:PRK05776 496 RIIETLFKRGYLTSNGKYIEVTPLGFGVAEVLekyfPDI-VSVELTRDFEEKLEMIRTGKATREEVIEEAKETLNKLLEE 574
|
650 660
....*....|....*....|....*....
gi 446687820 613 ANSAEIKVSPS-APSGQSQRLSSPCPSCG 640
Cdd:PRK05776 575 FKKNKDEIGEElAKALGLIKPVGKCKICG 603
|
|
| PRK14973 |
PRK14973 |
DNA topoisomerase I; Provisional |
1-659 |
1.55e-39 |
|
DNA topoisomerase I; Provisional
Pssm-ID: 184936 [Multi-domain] Cd Length: 936 Bit Score: 156.93 E-value: 1.55e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGGN---FTRHDG---WFESDNTIVTNCFGHIIESqppeNYNPEYKEWKIETLPLR-LYPVK 73
Cdd:PRK14973 1 MHLIIAEKNIAANRIAQILAGKtkvQVKKDGgvsTYSFDDTVVVGLRGHVVEV----DFEPGYTNWRSEEHTPRsLIDAD 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 74 YQPVESAAKQVKTIIELIRRADVTEIihAGDPDDEGQLLVDEVLEY--AGNTK-PVKRVLINDNTLPAVKKALANPkNNR 150
Cdd:PRK14973 77 TIKKPTEKKIVGLIQKLAKKADRVTI--ATDFDTEGELIGKEAYELvrAVNPKvPIDRARFSAITKEEIVTAFAEP-TDL 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 151 DFrGLYLKALARSVADAIYGLSMTRAYTIPAKtKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGAD 230
Cdd:PRK14973 154 DF-ALAAAGEARQIIDLIWGASLTRFISLAAH-RGGDNILSVGRVQSPTLAMIVDREKEIEAFVPEKYWMLSLATEKDGE 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 231 VIRANWKPGEFaplTDRKLLDKTWAnGTAtslagKPATVEAAATDDKKTAAPLPFNLVrlqQYM--NKKFKMTAQKTLDI 308
Cdd:PRK14973 232 GIEARHTHGRF---TDSAAAEAAYD-ATK-----EPLVVTEVKEGHKVDRAPTPFDTT---TFIvaASRLGFSAANAMRI 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 309 TQQLREKyKAITYNRSDCS-YLSDEQFSEAPQIIDalKSVFDQPMD-IDTTRKSKAFNSAK-VTAHTAIIPTvSVPDVNA 385
Cdd:PRK14973 300 AEDLYMN-GYISYPRTDNTiYPKSLDLNGVLATLA--KGAFSKDVAwVKDNRRPVPTRGKKsSTDHPPIHPT-GVATREE 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 386 LSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDdeaegenddsafDLLCK 465
Cdd:PRK14973 376 LGDDRWKLYELVVRRFLATLSPDAEWATMKVNFDAGGEPYTATGGRLLEAGWRTVYPYSEAKE------------NILPA 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 466 IRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASILETLKKR 545
Cdd:PRK14973 444 FALGEKLPILAVNLEEKETQPPARYSQSRLIQRM-------------------------EELGLGTKSTRHEVIGKLVSR 498
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 546 NYItlEKGKLIPTDTGYALIDALPDIA---VNPDMTALWAEKQTLIENGEMTIEQFVDE-------LYNDLipmisNANS 615
Cdd:PRK14973 499 KYI--EGNPLRPTLVGRAVTESLEEHAgtiTEPDMTQTLEEHMQQIKERKRTRDDVVTEsrkmlhrAFDEL-----EANE 571
|
650 660 670 680 690
....*....|....*....|....*....|....*....|....*....|.
gi 446687820 616 AEIKVSPSAPSGQSQRLsSPCPSCGKQIVIRPKSYS----CTG---CEFKI 659
Cdd:PRK14973 572 AVIGRDIMERTAEELTI-GPCPVCGKDLRIKHIGSSqfigCSGypdCTFNI 621
|
|
| PRK06319 |
PRK06319 |
DNA topoisomerase I/SWI domain fusion protein; Validated |
1-613 |
2.08e-29 |
|
DNA topoisomerase I/SWI domain fusion protein; Validated
Pssm-ID: 235778 [Multi-domain] Cd Length: 860 Bit Score: 124.93 E-value: 2.08e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MK--LFIAEKPAVANDIVKALGGNFTrhdgwFESDntivtncFGHIIESqppenynPEyKEWKIEtLPLRLYPvKYQPVE 78
Cdd:PRK06319 1 MKksLIIVESPAKIKTLQKLLGEGFI-----FASS-------LGHIVDL-------PA-KEFGID-IENDFEP-DYQILP 58
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 79 SAAKQVKTIIELIRRADVTEIihAGDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPknnRDFRGLYLK 158
Cdd:PRK06319 59 DKEEVINKICKLAKKCDVVYL--SPDPDREGEAIAWHIANQLPKNTKIQRISFNAITKGAVTEALKHP---REIDMALVN 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 159 AL-ARSVADAIYGLSMTrayTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRG-------AD 230
Cdd:PRK06319 134 AQqARRLLDRIVGYKIS---PILSRKLQRRSGVSAGRVQSVALKLVVDREKAIEAFVPVEYWNIRVHLKDPktqktfwAH 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 231 VIRANWKPGEFAPLTDRKLLDKTWANGTATS------LAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQK 304
Cdd:PRK06319 211 LYSVDGKKWEKEIPEGKTEDEVLLINSKEKAdhivelLESATYTVTRVESKEKRRNAYPPFITSTLQQEASRHFRFSSSR 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 305 TLDITQQLREKYKA--------ITYNRSDCSYLSDEQFSEAPQIIdalKSVFDQPMdidTTRKSKAFNSAKVT--AHTAI 374
Cdd:PRK06319 291 TMNIAQTLYEGVDLdsegatglITYMRTDSVRTDPEALKQVRKYI---EGTFGKEF---LPSSPNVYTTKKMAqdAHEAI 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 375 IPT--VSVPDV--NALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDEsFYARArkttdSG----FEAFLGV--E 444
Cdd:PRK06319 365 RPTdiTLTPEKlrSKLTEDQYKLYSLIWKRFVASQMIPAIYDTLAIRITTNKG-IDLRA-----TGsllkFKGFLAVyeE 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 445 NAGDDEAEGENDdsafdLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkd 524
Cdd:PRK06319 439 KRDDEGDEEENI-----HLPKLHEQDVLTKEELSAEQAFTKPLPRFTEASLVKEL------------------------- 488
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 525 EHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYALIDAL----PDIaVNPDMTALWAEKQTLIENGEMTIEQFVD 600
Cdd:PRK06319 489 EKSGIGRPSTYATIMNKIQSREYTLKENQRLRPTELGKIISQFLetnfPRI-MDIGFTALMEDELELIADNKKPWKLLLQ 567
|
650
....*....|...
gi 446687820 601 ELYNDLIPMISNA 613
Cdd:PRK06319 568 EFWELFLPVVVTA 580
|
|
| TopG2 |
COG1110 |
Reverse gyrase [Replication, recombination and repair]; |
86-617 |
1.85e-20 |
|
Reverse gyrase [Replication, recombination and repair];
Pssm-ID: 440727 [Multi-domain] Cd Length: 1219 Bit Score: 96.97 E-value: 1.85e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 86 TIIELIRR--ADVTEIIHAGDPDDEGQLLV-DEVLEYAGNTKPVKRVLINDNTLPAVKKALANPknnRDFRGLYLKA-LA 161
Cdd:COG1110 739 EIVEALRElaTEVDEVLIGTDPDTEGEKIGwDVYNLLRPYAKNIKRIEFHEVTRKAILEALKNP---REIDENLVEAqIV 815
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 162 RSVADAIYGLSMTraytiPAKTKGY-KGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYytmtghfqrgadVIRANWKPGE 240
Cdd:COG1110 816 RRIEDRWIGFTLS-----QKLWKVFgNRNLSAGRVQTPVLGWIIERYEESRKSKKYLI------------ILTLENGRLE 878
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 241 FaPLTDRKLLDKTwanGTATSLAGKPATVEAAATDDKKTAAPlPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKyKAIT 320
Cdd:COG1110 879 L-SIEDLKEAEEV---RKALEKEVKVKVKKVEEEEEELNPLP-PYTTDTLLRDASRRLGLSAEETMRLAQDLFES-GLIT 952
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 321 YNRSDCSYLSDEQFSEAPQIIdalksvfdqpmdidttrkSKAFNSAKVT--------AHTAIIPT--VSVPDVNALsTDE 390
Cdd:COG1110 953 YHRTDSTRVSDAGIAVAKEYI------------------SERFGEEYFKprrwgeggAHEAIRPTrpLDAEDLRRL-IEE 1013
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 391 RNVYLAIA---QHYLV------QFMP--------EKAYQEVSVAIQcGDESFYARARKTTDSGFEAFLGVENAGDDEAEG 453
Cdd:COG1110 1014 GVIELPIPltrRHLRLydlifrRFMAsqmrpakvEKVKLRVSLELP-GGEIEEERIVEIIEDGFLLIYPPIKVEPELKEG 1092
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 454 EnddsafdllckirtgetVTTKEVVVneKKTTPPPLFTEATLLAALVRVadfvtdpvikkllkdkdrdkkdehgGIGTPA 533
Cdd:COG1110 1093 E-----------------YKVTEVKI--RKVPKVPLYTQGDLVREMKER-------------------------GIGRPS 1128
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 534 TRASILETLKKRNYITLEK--GKLIPTDTGYA----LIDALPDIaVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLI 607
Cdd:COG1110 1129 TYAKIVETLLRRGYVVESKkgKKLVPTKLGIEvynyLSENFPDL-VSEERTRELEEKMDEIEEGEADYQDVLEELYNELR 1207
|
570
....*....|
gi 446687820 608 PMISNANSAE 617
Cdd:COG1110 1208 ELVLSPESEL 1217
|
|
| TOP1Bc |
smart00436 |
Bacterial DNA topoisomeraes I ATP-binding domain; Extension of TOPRIM in Bacterial DNA ... |
126-220 |
2.50e-20 |
|
Bacterial DNA topoisomeraes I ATP-binding domain; Extension of TOPRIM in Bacterial DNA topoisomeraes I and III, Eukaryotic DNA topoisomeraes III, reverse gyrase beta subunit
Pssm-ID: 214662 [Multi-domain] Cd Length: 89 Bit Score: 85.71 E-value: 2.50e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 126 VKRVLINDNTLPAVKKALANPKNNrDFrGLYLKALARSVADAIYGLSMTRAYTipakTKGYKGVLSVGRVQTPVLGLIVN 205
Cdd:smart00436 1 IKRIEFSEITKKAIREALKNPREI-DE-NLVNAQLARRILDRLIGFNLSRLLT----KKLRNGVLSAGRVQTPTLGLIVE 74
|
90
....*....|....*
gi 446687820 206 RTRANKNHKSSFYYT 220
Cdd:smart00436 75 REREIKNFVPKPYWE 89
|
|
| Toprim_Crpt |
pfam13342 |
C-terminal repeat of topoisomerase; |
654-715 |
7.61e-18 |
|
C-terminal repeat of topoisomerase;
Pssm-ID: 463851 [Multi-domain] Cd Length: 60 Bit Score: 77.90 E-value: 7.61e-18
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446687820 654 GCEFKIWKDFCGKSLTDKQIETLITKGVTSELKGFISqKTNKEFSAKVKLVDKatGKLGFEF 715
Cdd:pfam13342 1 GCGFSIGKTIAGKKLSEEQVKELLTKGKTGLIKGFKS-KKGKKFDAYLVLDDD--EKVEFDF 59
|
|
| PTZ00407 |
PTZ00407 |
DNA topoisomerase IA; Provisional |
95-566 |
8.03e-17 |
|
DNA topoisomerase IA; Provisional
Pssm-ID: 173597 [Multi-domain] Cd Length: 805 Bit Score: 84.99 E-value: 8.03e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 95 DVTEIIHAGDPDDEGQLLVDEVLE-----YAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKALARSVADAIY 169
Cdd:PTZ00407 124 NVTEIILATDPDREGELIAVHALQtikrlYPKLKVPFSRAYMHSITEDGIRKAMRERHVEACDYDLANAAETRHAMDRIF 203
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 170 GL---SMTRAYTIPAKtkgykgvlSVGRVQTPVLGLIVNRTRANK----NHKSSFYYTMTGHF-----QRGADVIRAN-W 236
Cdd:PTZ00407 204 GFlgsSVVRAANSQMR--------SIGRVQTPALILINEREDKIKafleSNKSTFEVQAMCQFpsphgTTFSQVVTITpD 275
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 237 KPGEFAPLTDRKLLDKT---WANGTATSLAGKPatveAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLR 313
Cdd:PTZ00407 276 RKGGASHWATEAEARRCleqWKLNNCTGFSVPL----EPKPQPSVVPPPQPFTMATAIAKANRQLKYSSEMVSGCLQDLF 351
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 314 EkYKAITYNRSDCSYLsDEqfSEAPQIIDALKSVF---------DQPMDIDTTRKSKAFNSAKVT--------------- 369
Cdd:PTZ00407 352 Q-LGHITYPRTDSTRI-DE--SALPDIYAAVKKEFgkeflyrleDRTVSAQEGKGSKKTGKKRSTkqkkgadtpvgnved 427
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 370 AHTAIIPTVSVPDVN--ALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQC----GDE-SFYARARKTTDSGFEAFL- 441
Cdd:PTZ00407 428 AHEAIRPTNIDTTGEslSLSPPTRAVYDLVRRNTLAVFMIPMKTEKIVATVKFtsgsGEKlEFELQGKRVVEPGWTRAFh 507
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 442 -GVENAGDDEAEGENDDSAFD----LLCKIRTGE------------------TVTTKEVVVNEKKTTPPPLFTEATLLAA 498
Cdd:PTZ00407 508 kGDKGTTGPAEETDEDITAVEegapVVPSLSQEEfkaimnlrsqlgsgvqkgFFELRSPQVRENRPVPPLPHSEGTLIEE 587
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446687820 499 LvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASILETLKKRNYITL-EKGKLIPTDTGYALID 566
Cdd:PTZ00407 588 L-------------------------KNNGVGRPSTYPMIVKTLLARGYIAVnPKGRCETTPVGRMLVE 631
|
|
| YrdD |
COG0551 |
DNA topoisomerase I, ssDNA-binding Zn-finger and Zn-ribbon domains [Replication, recombination ... |
72-646 |
2.55e-12 |
|
DNA topoisomerase I, ssDNA-binding Zn-finger and Zn-ribbon domains [Replication, recombination and repair];
Pssm-ID: 440317 [Multi-domain] Cd Length: 695 Bit Score: 70.28 E-value: 2.55e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 72 VKYQPVESAAKQVKTIIELIRRADVTEIIHAGDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRD 151
Cdd:COG0551 58 ENEKLIAELDIKDLKKLKKDKKKKAKALAAALAAADDDAIEAAAAADEEEALKAAEALEAIRKAVVEEVKSRARKRAARA 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 152 FRGLYLKALARSVADAIYglsmtRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFYYTMTGHFQRGADV 231
Cdd:COG0551 138 PADVAAAAAAAALARLLL-----LAAASRALRLLLLLAAGRLRLLSAVAAGLLELEELLKEFEEEEEEEAAEEEEEEEEA 212
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 232 IRANWKPGEFAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDDKKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQ 311
Cdd:COG0551 213 KKKKEEDKKKKKKKKKKDKEEETKAIKKKKKAAEAVLAAKKAAKEAKAKKELPTTTTDALPEALATTSLTAAAAASALAA 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 312 LR-----EKYKAITYNRSDCSYLSDEQFSEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNAL 386
Cdd:COG0551 293 ALleeegELLYTGGYIRYTRTPKSSEADALAALEALKKIAEEPPDERAKKKKKKAKKKKASEAKETTDHPPIPPTTAAAL 372
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 387 STDERnVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDEAEGEnddsafdLLCKI 466
Cdd:COG0551 373 LELLE-DRKLLLELLVRRRFAAAADLAKVEELTKVEEADEEELVEAEGGLLEVLGEEEEDDEDEEEEL-------LLPPL 444
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 467 RTGETVTTKEVVVNEKKTTPPPLFTEATLLAALvrvadfvtdpvikkllkdkdrdkkdEHGGIGTPATRASILETLKKRN 546
Cdd:COG0551 445 EEGEPLLLEELLLEKQTTTPPPRYTTELLLKKL-------------------------EEEGGGGRGTTATIIILILKRR 499
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 547 YITLEKGKLIPTDTGYALIDALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEIKVSPSAPS 626
Cdd:COG0551 500 YVVLEEKKTTPTELLVEVVLLLLLPFPKLEDTTETAEEEEALLDKEEGKEDKVVEIREEEYPFFKKLLEIAEEEGEEELS 579
|
570 580
....*....|....*....|
gi 446687820 627 GQSQRLSSPCPSCGKQIVIR 646
Cdd:COG0551 580 EEDEVLGEKCPKCGKPLVIK 599
|
|
| TOPRIM_TopoIA_TopoI |
cd03363 |
TOPRIM_TopoIA_TopoI: The topoisomerase-primase (TOPRIM) domain found in members of the type IA ... |
1-146 |
7.02e-11 |
|
TOPRIM_TopoIA_TopoI: The topoisomerase-primase (TOPRIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to Escherichia coli DNA topoisomerase I. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving one strand of the DNA duplex, covalently linking to the 5' phosphoryl end of the DNA break and, allowing the other strand of the duplex to pass through the gap. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). For topoisomerases the conserved glutamate is believed to act as a general base in strand joining and, as a general acid in strand cleavage. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function.
Pssm-ID: 173783 [Multi-domain] Cd Length: 123 Bit Score: 60.27 E-value: 7.02e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGGNFtrhdgwfesdntIVTNCFGHIIESQPPENYNP-EYKEWKietlplrlyPvKYQPVES 79
Cdd:cd03363 1 KKLVIVESPAKAKTIKKYLGKEY------------EVLASVGHIRDLPKKGLGVDgEDDGFE---------P-KYVVIPG 58
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446687820 80 AAKQVKTIIELIRRADvtEIIHAGDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANP 146
Cdd:cd03363 59 KKKVVKELKKLAKKAD--EIYLATDPDREGEAIAWHLAEVLKLKKNVKRVVFNEITKEAIKEALKNP 123
|
|
| COG1754 |
COG1754 |
Uncharacterized C-terminal domain of topoisomerase IA [Function unknown]; |
49-561 |
2.23e-10 |
|
Uncharacterized C-terminal domain of topoisomerase IA [Function unknown];
Pssm-ID: 441360 [Multi-domain] Cd Length: 892 Bit Score: 64.07 E-value: 2.23e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 49 PPENYNPEYKEWKIETLPLRLYPVKYQPVESAAKQVKTIIELIRRADvtEIIHAGDPDDEGQLLVDEVLEyagnTKPVKR 128
Cdd:COG1754 44 KADDKVVDDDDKKDDVDDDKKKKKKLLKAAAKKKKALALALAADDED--EELLAADDELEEEKVIKLLLL----ELAVER 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 129 VLINDNTlpAVKKALANPKNNRDFRGLYLKALARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTR 208
Cdd:COG1754 118 VARAAAV--RAAAAEAARLAALLRRRLVLRRLVRRLLVRLLLLRLVLLLLRLLVARRAARRRRRRRRRRARRRELERRRE 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 209 AnknhksSFYYTMTGHFQRGADVIRANWKPG--EFAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDDKKTAAPLPFN 286
Cdd:COG1754 196 R------ARAAEAEEAAAAAAAAADAGRAAAarDGKGGGKLAAGGAAAAAAAAAAAAAAAAALAEAAAAAAVREPPTPTT 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 287 LVRLQQYMNKKFKMTAQKTLDITQQLREK-------YKAITYNRSDCSYLSDEQFSEAPQIIDALKSVFDQPMDIDTTRK 359
Cdd:COG1754 270 TTTAQAATTTTTRAAAARTTRSAQQAARRtrlggegTYTTTTTTTTTTSAAAAAAAAAAAALAAAAAAAYAPPPPYYYKK 349
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 360 SKAFNSAKVTAHTAIIPTVSVPDVNALSTDERNVYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEA 439
Cdd:COG1754 350 KAAAAAAAAAAAAAAAARRPPAARAALLSDDRLLLELLLLRRTAAAAAAAAAATATTTAAAAAAGAGAVGTAAAGAVFFF 429
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 440 FLGVENAGDDEAEGENDDSafDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVadfvtdpvikkllkdkd 519
Cdd:COG1754 430 GGFLLFAEEEDDDEEDDDD--DDLPALEEGLPLLKEGVLADQHFTQPPPRYTEAPLVKRMEEL----------------- 490
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 446687820 520 rdkkdehgGIGTPATRASILETLKKRNYITLEKGKLIPTDTG 561
Cdd:COG1754 491 --------GIGRPSTYASILSTLADRIYVRLDKRRLIPEDKG 524
|
|
| Toprim |
pfam01751 |
Toprim domain; This is a conserved region from DNA primase. This corresponds to the Toprim ... |
2-132 |
9.20e-06 |
|
Toprim domain; This is a conserved region from DNA primase. This corresponds to the Toprim domain common to DnaG primases, topoisomerases, OLD family nucleases and RecR proteins. Both DnaG motifs IV and V are present in the alignment, the DxD (V) motif may be involved in Mg2+ binding and mutations to the conserved glutamate (IV) completely abolish DnaG type primase activity. DNA primase EC:2.7.7.6 is a nucleotidyltransferase it synthesizes the oligoribonucleotide primers required for DNA replication on the lagging strand of the replication fork; it can also prime the leading stand and has been implicated in cell division. This family also includes the atypical archaeal A subunit from type II DNA topoisomerases. Type II DNA topoisomerases catalyze the relaxation of DNA supercoiling by causing transient double strand breaks.
Pssm-ID: 396354 [Multi-domain] Cd Length: 93 Bit Score: 44.65 E-value: 9.20e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 2 KLFIAEKPAVANDIVKALGGNFTRhdgwfesdntiVTNCFGHIIEsqppenynpeykewkietlplrlypvkyQPVESAA 81
Cdd:pfam01751 1 ELIIVEGPSDAIALEKALGGGFQA-----------VVAVLGHLLS----------------------------LEKGPKK 41
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|..
gi 446687820 82 KQVKTIIELIRRADvtEIIHAGDPDDEGQLLVDEVLEYAG-NTKPVKRVLIN 132
Cdd:pfam01751 42 KALKALKELALKAK--EVILATDPDREGEAIALKLLELKElLENAGGRVEFS 91
|
|
| TOPRIM |
smart00493 |
topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins; |
1-122 |
1.87e-04 |
|
topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins;
Pssm-ID: 214695 [Multi-domain] Cd Length: 75 Bit Score: 40.32 E-value: 1.87e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446687820 1 MKLFIAEKPAVANDIVKALGgnftrhdgwfesDNTIVTNCFGHIiesqppenynpeykewkietlplrlypvkyqpveSA 80
Cdd:smart00493 1 KVLIIVEGPADAIALEKAGG------------KRGNVVALGGHL----------------------------------LS 34
|
90 100 110 120
....*....|....*....|....*....|....*....|..
gi 446687820 81 AKQVKTIIELIRRAdvtEIIHAGDPDDEGQLLVDEVLEYAGN 122
Cdd:smart00493 35 KEQIKLLKKLAKKA---EVILATDPDREGEAIAWELAELLKP 73
|
|
| TOPRIM_TopoIA_RevGyr |
cd03361 |
TopoIA_RevGyr : The topoisomerase-primase (TORPIM) domain found in members of the type IA ... |
85-146 |
8.75e-03 |
|
TopoIA_RevGyr : The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to the ATP-dependent reverse gyrase found in archaea and thermophilic bacteria. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving one strand of the DNA duplex, covalently linking to the 5' phosphoryl end of the DNA break and, allowing the other strand of the duplex to pass through the gap. Reverse gyrase is also able to insert positive supercoils in the presence of ATP and negative supercoils in the presence of AMPPNP. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). For topoisomerases the conserved glutamate is believed to act as a general base in strand joining and, as a general acid in strand cleavage. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function.
Pssm-ID: 173781 [Multi-domain] Cd Length: 170 Bit Score: 37.68 E-value: 8.75e-03
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446687820 85 KTIIELIRR--ADVTEIIHAGDPDDEGQLLVDEVLEYAG-NTKPVKRVLINDNTLPAVKKALANP 146
Cdd:cd03361 106 LETLEALRElaLEVDEVLIATDPDTEGEKIAWDVYLALRpYNKNIKRAEFHEVTRRAILEALRNP 170
|
|
| |
