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Conserved domains on  [gi|446693555|ref|WP_000770901|]
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MULTISPECIES: colanic acid biosynthesis pyruvyl transferase WcaK [Salmonella]

Protein Classification

glycosyltransferase family protein( domain architecture ID 56)

glycosyltransferase family protein may synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Glycosyltransferase_GTB-type super family cl10013
glycosyltransferase family 1 and related proteins with GTB topology; Glycosyltransferases ...
 1-426 0e+00

glycosyltransferase family 1 and related proteins with GTB topology; Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. The structures of the formed glycoconjugates are extremely diverse, reflecting a wide range of biological functions. The members of this family share a common GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.


The actual alignment was detected with superfamily member TIGR04006:

Pssm-ID: 471961  Cd Length: 426  Bit Score: 838.03  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555    1 MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL 80
Cdd:TIGR04006   1 MKLLILGNHTCGNRGDSAILRGLLDAINQLEPEAEVDVMSRYPVSSSWLLNRPVMGDPLYSQMKQHNNAAGVMGRVKKVL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   81 RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP 160
Cdd:TIGR04006  81 RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLYMIGHSVGP 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  161 FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT 240
Cdd:TIGR04006 161 FQNPQFNQLANYVFGHCDALILRESVSLDLMKRSQIDTSKVEHGVDTAWLVDHHNDDFVASYAVQHWLDVAARQKTVAIT 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  241 LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSEHFHIAMDELNDVE 320
Cdd:TIGR04006 241 LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVLALSTCTGIDSYNKDDRMVALNLRKLVNDPSRYHVVMDELNDLE 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  321 IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV 400
Cdd:TIGR04006 321 MGKILGACELTVGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPALNARL 400

                         410       420
                  ....*....|....*....|....*.
gi 446693555  401 ANAVTEERAKGFTMIKSVLDRIREAQ 426
Cdd:TIGR04006 401 ANAVTAEREKGFQMVKSVLDRIGEAK 426
 
Name Accession Description Interval E-value
wcaK TIGR04006
colanic acid biosynthesis pyruvyl transferase WcaK; This gene is the pyruvyl transferase ...
 1-426 0e+00

colanic acid biosynthesis pyruvyl transferase WcaK; This gene is the pyruvyl transferase involved in the biosynthesis of colanic acid, an exopolysaccharide expressed in Enterobacteraceae species.


Pssm-ID: 188521  Cd Length: 426  Bit Score: 838.03  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555    1 MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL 80
Cdd:TIGR04006   1 MKLLILGNHTCGNRGDSAILRGLLDAINQLEPEAEVDVMSRYPVSSSWLLNRPVMGDPLYSQMKQHNNAAGVMGRVKKVL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   81 RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP 160
Cdd:TIGR04006  81 RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLYMIGHSVGP 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  161 FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT 240
Cdd:TIGR04006 161 FQNPQFNQLANYVFGHCDALILRESVSLDLMKRSQIDTSKVEHGVDTAWLVDHHNDDFVASYAVQHWLDVAARQKTVAIT 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  241 LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSEHFHIAMDELNDVE 320
Cdd:TIGR04006 241 LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVLALSTCTGIDSYNKDDRMVALNLRKLVNDPSRYHVVMDELNDLE 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  321 IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV 400
Cdd:TIGR04006 321 MGKILGACELTVGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPALNARL 400

                         410       420
                  ....*....|....*....|....*.
gi 446693555  401 ANAVTEERAKGFTMIKSVLDRIREAQ 426
Cdd:TIGR04006 401 ANAVTAEREKGFQMVKSVLDRIGEAK 426
PRK10017 PRK10017
colanic acid biosynthesis protein; Provisional
 1-426 0e+00

colanic acid biosynthesis protein; Provisional


Pssm-ID: 182196  Cd Length: 426  Bit Score: 793.97  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   1 MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL 80
Cdd:PRK10017   1 MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPVSSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  81 RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP 160
Cdd:PRK10017  81 RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCAFMAKKPLYMIGHSVGP 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 161 FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT 240
Cdd:PRK10017 161 FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT 240

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 241 LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSEHFHIAMDELNDVE 320
Cdd:PRK10017 241 LRELAPFDKRLGTTQQAYEKAFAGVVNRIIDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHVSDPARYHVVMDELNDLE 320

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 321 IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV 400
Cdd:PRK10017 321 MGKILGACELTVGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPALNARL 400

                        410       420
                 ....*....|....*....|....*.
gi 446693555 401 ANAVTEERAKGFTMIKSVLDRIREAQ 426
Cdd:PRK10017 401 AEAVSRERQTGMQMVQSVLERIGEVK 426
WcaK COG2327
Polysaccharide pyruvyl transferase family protein WcaK (colanic acid biosynthesis) [Cell wall ...
 1-423 9.27e-74

Polysaccharide pyruvyl transferase family protein WcaK (colanic acid biosynthesis) [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441900 [Multi-domain]  Cd Length: 357  Bit Score: 234.95  E-value: 9.27e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   1 MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGrpiiadplyqlsqkqqaaaglngrVKKVL 80
Cdd:COG2327    1 MKILLSGYYGFGNLGDEALLEALLQLLRKRLPDAEITVLSGNPEETRKRYG------------------------VRAVP 56

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  81 RRRFqhkillskvaqegslrnfaiapefAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPL----CAFMANKPIYMVGH 156
Cdd:COG2327   57 RRNF------------------------LEILRALRRADLVISGGGGLLQDVTSLRSLLYYLglvlLAKLLGKPVVIYGQ 112

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 157 SVGPFQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEfvasyavqhwLDVTARRKT 236
Cdd:COG2327  113 GIGPFKSPLSRRLLRRVLNRADLISVRDEASAELLKDLGVKKPPIELTPDPAFLLPPEPVD----------KEAPLERPV 182

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 237 VAITLRDLEPFDkrlgttqQAYEQAFAQVVNRLIAD-GYQVLALSTCTSIDrynrDDRMVALRMAKYVndSEHFHIAMDE 315
Cdd:COG2327  183 VGVNLRPWRGLE-------EDYLEALAAALDRLIERtGYKVVLLPHQYGQE----DDLAIAEELAAKL--KERVVVLDEE 249

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 316 LNDVEIGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPA 395
Cdd:COG2327  250 LDPDELLSLIARADLVIGMRLHALIFALSAGVPVIALSYDPKVRGLMEDLGLPDYVLDLDDLDPEELLAAVEELLANREE 329

                        410       420
                 ....*....|....*....|....*...
gi 446693555 396 LKAKVANAVTEERAKGFTMIKSVLDRIR 423
Cdd:COG2327  330 LREKLAEKLAELRARARENLELLLKLLK 357
PS_pyruv_trans pfam04230
Polysaccharide pyruvyl transferase; Pyruvyl-transferases involved in peptidoglycan-associated ...
 13-356 2.77e-18

Polysaccharide pyruvyl transferase; Pyruvyl-transferases involved in peptidoglycan-associated polymer biosynthesis. CsaB in Bacillus anthracis is necessary for the non-covalent anchoring of proteins containing an SLH (S-layer homology) domain to peptidoglycan-associated pyruvylated polysaccharides. WcaK and AmsJ are involved in the biosynthesis of colanic acid in Escherichia coli and of amylovoran in Erwinia amylovora.


Pssm-ID: 427804  Cd Length: 233  Bit Score: 83.45  E-value: 2.77e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   13 NRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLqgrpiiadplyqlsqkqqaaaglnGRVKKVLRRRFQHKILLsk 92
Cdd:pfam04230   1 NYGDELILEALLKLLKKLGPDAEIIVLSPDPDETKKR------------------------LSDKRSKKLKLLKKLLK-- 54

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   93 vaqegslrnfaiapefaefaqFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMA---NKPIYMVGHSVGPFQNPDFNQL 169
Cdd:pfam04230  55 ---------------------LLDKADDVIIVGGGLGNLGDLYKLLIYLRLAFVAkklGKPVILLGQGIGPFLNEALRKL 113

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  170 ANYVFGRTSALILRETVSRDLMTAAQIDtRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTarrktvaitlrdlepfdK 249
Cdd:pfam04230 114 LKKILSKADLISVRDELSYELLEKLGIK-ENVELVPDPAFLLDKLPPKEIKKKPKKDYISIA-----------------K 175

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  250 RLGTTQQAYEQAFAQVVNRLIadgyqvlalstctsidrynrddrmvalrmakyvndsehfhiamdelndvEIGKILASCV 329
Cdd:pfam04230 176 SSKKDLEDLNEKLALLYDLPE-------------------------------------------------ELLSLISQAD 206

                         330       340
                  ....*....|....*....|....*..
gi 446693555  330 LTIGTRLHSAIISMNFGTPAIAINYEH 356
Cdd:pfam04230 207 FVITTRLHGLILALLFGVPFIVIAYDS 233
 
Name Accession Description Interval E-value
wcaK TIGR04006
colanic acid biosynthesis pyruvyl transferase WcaK; This gene is the pyruvyl transferase ...
 1-426 0e+00

colanic acid biosynthesis pyruvyl transferase WcaK; This gene is the pyruvyl transferase involved in the biosynthesis of colanic acid, an exopolysaccharide expressed in Enterobacteraceae species.


Pssm-ID: 188521  Cd Length: 426  Bit Score: 838.03  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555    1 MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL 80
Cdd:TIGR04006   1 MKLLILGNHTCGNRGDSAILRGLLDAINQLEPEAEVDVMSRYPVSSSWLLNRPVMGDPLYSQMKQHNNAAGVMGRVKKVL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   81 RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP 160
Cdd:TIGR04006  81 RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLYMIGHSVGP 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  161 FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT 240
Cdd:TIGR04006 161 FQNPQFNQLANYVFGHCDALILRESVSLDLMKRSQIDTSKVEHGVDTAWLVDHHNDDFVASYAVQHWLDVAARQKTVAIT 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  241 LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSEHFHIAMDELNDVE 320
Cdd:TIGR04006 241 LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVLALSTCTGIDSYNKDDRMVALNLRKLVNDPSRYHVVMDELNDLE 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  321 IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV 400
Cdd:TIGR04006 321 MGKILGACELTVGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPALNARL 400

                         410       420
                  ....*....|....*....|....*.
gi 446693555  401 ANAVTEERAKGFTMIKSVLDRIREAQ 426
Cdd:TIGR04006 401 ANAVTAEREKGFQMVKSVLDRIGEAK 426
PRK10017 PRK10017
colanic acid biosynthesis protein; Provisional
 1-426 0e+00

colanic acid biosynthesis protein; Provisional


Pssm-ID: 182196  Cd Length: 426  Bit Score: 793.97  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   1 MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL 80
Cdd:PRK10017   1 MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPVSSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  81 RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP 160
Cdd:PRK10017  81 RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCAFMAKKPLYMIGHSVGP 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 161 FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT 240
Cdd:PRK10017 161 FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT 240

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 241 LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSEHFHIAMDELNDVE 320
Cdd:PRK10017 241 LRELAPFDKRLGTTQQAYEKAFAGVVNRIIDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHVSDPARYHVVMDELNDLE 320

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 321 IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV 400
Cdd:PRK10017 321 MGKILGACELTVGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPALNARL 400

                        410       420
                 ....*....|....*....|....*.
gi 446693555 401 ANAVTEERAKGFTMIKSVLDRIREAQ 426
Cdd:PRK10017 401 AEAVSRERQTGMQMVQSVLERIGEVK 426
WcaK COG2327
Polysaccharide pyruvyl transferase family protein WcaK (colanic acid biosynthesis) [Cell wall ...
 1-423 9.27e-74

Polysaccharide pyruvyl transferase family protein WcaK (colanic acid biosynthesis) [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441900 [Multi-domain]  Cd Length: 357  Bit Score: 234.95  E-value: 9.27e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   1 MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGrpiiadplyqlsqkqqaaaglngrVKKVL 80
Cdd:COG2327    1 MKILLSGYYGFGNLGDEALLEALLQLLRKRLPDAEITVLSGNPEETRKRYG------------------------VRAVP 56

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  81 RRRFqhkillskvaqegslrnfaiapefAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPL----CAFMANKPIYMVGH 156
Cdd:COG2327   57 RRNF------------------------LEILRALRRADLVISGGGGLLQDVTSLRSLLYYLglvlLAKLLGKPVVIYGQ 112

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 157 SVGPFQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEfvasyavqhwLDVTARRKT 236
Cdd:COG2327  113 GIGPFKSPLSRRLLRRVLNRADLISVRDEASAELLKDLGVKKPPIELTPDPAFLLPPEPVD----------KEAPLERPV 182

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 237 VAITLRDLEPFDkrlgttqQAYEQAFAQVVNRLIAD-GYQVLALSTCTSIDrynrDDRMVALRMAKYVndSEHFHIAMDE 315
Cdd:COG2327  183 VGVNLRPWRGLE-------EDYLEALAAALDRLIERtGYKVVLLPHQYGQE----DDLAIAEELAAKL--KERVVVLDEE 249

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555 316 LNDVEIGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPA 395
Cdd:COG2327  250 LDPDELLSLIARADLVIGMRLHALIFALSAGVPVIALSYDPKVRGLMEDLGLPDYVLDLDDLDPEELLAAVEELLANREE 329

                        410       420
                 ....*....|....*....|....*...
gi 446693555 396 LKAKVANAVTEERAKGFTMIKSVLDRIR 423
Cdd:COG2327  330 LREKLAEKLAELRARARENLELLLKLLK 357
PS_pyruv_trans pfam04230
Polysaccharide pyruvyl transferase; Pyruvyl-transferases involved in peptidoglycan-associated ...
 13-356 2.77e-18

Polysaccharide pyruvyl transferase; Pyruvyl-transferases involved in peptidoglycan-associated polymer biosynthesis. CsaB in Bacillus anthracis is necessary for the non-covalent anchoring of proteins containing an SLH (S-layer homology) domain to peptidoglycan-associated pyruvylated polysaccharides. WcaK and AmsJ are involved in the biosynthesis of colanic acid in Escherichia coli and of amylovoran in Erwinia amylovora.


Pssm-ID: 427804  Cd Length: 233  Bit Score: 83.45  E-value: 2.77e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   13 NRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLqgrpiiadplyqlsqkqqaaaglnGRVKKVLRRRFQHKILLsk 92
Cdd:pfam04230   1 NYGDELILEALLKLLKKLGPDAEIIVLSPDPDETKKR------------------------LSDKRSKKLKLLKKLLK-- 54

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555   93 vaqegslrnfaiapefaefaqFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMA---NKPIYMVGHSVGPFQNPDFNQL 169
Cdd:pfam04230  55 ---------------------LLDKADDVIIVGGGLGNLGDLYKLLIYLRLAFVAkklGKPVILLGQGIGPFLNEALRKL 113

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  170 ANYVFGRTSALILRETVSRDLMTAAQIDtRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTarrktvaitlrdlepfdK 249
Cdd:pfam04230 114 LKKILSKADLISVRDELSYELLEKLGIK-ENVELVPDPAFLLDKLPPKEIKKKPKKDYISIA-----------------K 175

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693555  250 RLGTTQQAYEQAFAQVVNRLIadgyqvlalstctsidrynrddrmvalrmakyvndsehfhiamdelndvEIGKILASCV 329
Cdd:pfam04230 176 SSKKDLEDLNEKLALLYDLPE-------------------------------------------------ELLSLISQAD 206

                         330       340
                  ....*....|....*....|....*..
gi 446693555  330 LTIGTRLHSAIISMNFGTPAIAINYEH 356
Cdd:pfam04230 207 FVITTRLHGLILALLFGVPFIVIAYDS 233
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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