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Conserved domains on  [gi|446693928|ref|WP_000771274|]
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MULTISPECIES: GNAT family N-acetyltransferase [Bacillus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10008168)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl coenzyme A to a substrate

CATH:  3.40.630.30
EC:  2.3.1.-
Gene Ontology:  GO:0008080
PubMed:  9175471|10940244|15581578
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
COG3981 COG3981
Predicted acetyltransferase [General function prediction only];
1-170 1.04e-74

Predicted acetyltransferase [General function prediction only];


:

Pssm-ID: 443180  Cd Length: 170  Bit Score: 220.94  E-value: 1.04e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928   1 MKLLKPIHEYSEHITAYRQAFLHSGEqpHGSSSLQNFDSLDEWFEKVSKQELGENLLANRVPSSQFLSF-EKGKLIGFVN 79
Cdd:COG3981    2 MELVRPTLEDEESYLEYLAEFLKEHI--DGSGYLVSFEDFEAWLERLLDEEKGEELPEGWVPATTYWLVdEDGRIVGAIN 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928  80 IRHRLNPELLRESGHIGYSVHPNKRRHGYATKQLQLALAEAQKLGLQKVLITCDKSNIGSAKTIQKVGGVLENEVVSSRT 159
Cdd:COG3981   80 LRHELNEFLLRVGGHIGYGVRPSERGKGYATEMLRLALEEARELGLDRVLITCDKDNIASRKVIEANGGVLEDEVVDEED 159

                        170
                 ....*....|.
gi 446693928 160 GEVIQRYWIEI 170
Cdd:COG3981  160 GRPVRRYWIDL 170
 
Name Accession Description Interval E-value
COG3981 COG3981
Predicted acetyltransferase [General function prediction only];
1-170 1.04e-74

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443180  Cd Length: 170  Bit Score: 220.94  E-value: 1.04e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928   1 MKLLKPIHEYSEHITAYRQAFLHSGEqpHGSSSLQNFDSLDEWFEKVSKQELGENLLANRVPSSQFLSF-EKGKLIGFVN 79
Cdd:COG3981    2 MELVRPTLEDEESYLEYLAEFLKEHI--DGSGYLVSFEDFEAWLERLLDEEKGEELPEGWVPATTYWLVdEDGRIVGAIN 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928  80 IRHRLNPELLRESGHIGYSVHPNKRRHGYATKQLQLALAEAQKLGLQKVLITCDKSNIGSAKTIQKVGGVLENEVVSSRT 159
Cdd:COG3981   80 LRHELNEFLLRVGGHIGYGVRPSERGKGYATEMLRLALEEARELGLDRVLITCDKDNIASRKVIEANGGVLEDEVVDEED 159

                        170
                 ....*....|.
gi 446693928 160 GEVIQRYWIEI 170
Cdd:COG3981  160 GRPVRRYWIDL 170
Acetyltransf_3 pfam13302
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 38-147 1.15e-10

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 379112 [Multi-domain]  Cd Length: 139  Bit Score: 56.20  E-value: 1.15e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928   38 DSLDEWFEKVSKQelgenllANRVPSSQFLSFEKG-KLIGFVNIRHRLNPellRESGHIGYSVHPNKRRHGYATKQLQLA 116
Cdd:pfam13302  36 EEAREWLARIWAA-------DEAERGYGWAIELKDtGFIGSIGLYDIDGE---PERAELGYWLGPDYWGKGYATEAVRAL 105

                          90       100       110
                  ....*....|....*....|....*....|..
gi 446693928  117 LAEA-QKLGLQKVLITCDKSNIGSAKTIQKVG 147
Cdd:pfam13302 106 LEYAfEELGLPRLVARIDPENTASRRVLEKLG 137
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 66-131 2.55e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 40.34  E-value: 2.55e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446693928  66 FLSFEKGKLIGFVNIRHRlnpELLRESGHIGY-SVHPNKRRHGYATKQLQLALAEAQKLGLQKVLIT 131
Cdd:cd04301    2 LVAEDDGEIVGFASLSPD---GSGGDTAYIGDlAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
 
Name Accession Description Interval E-value
COG3981 COG3981
Predicted acetyltransferase [General function prediction only];
1-170 1.04e-74

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443180  Cd Length: 170  Bit Score: 220.94  E-value: 1.04e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928   1 MKLLKPIHEYSEHITAYRQAFLHSGEqpHGSSSLQNFDSLDEWFEKVSKQELGENLLANRVPSSQFLSF-EKGKLIGFVN 79
Cdd:COG3981    2 MELVRPTLEDEESYLEYLAEFLKEHI--DGSGYLVSFEDFEAWLERLLDEEKGEELPEGWVPATTYWLVdEDGRIVGAIN 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928  80 IRHRLNPELLRESGHIGYSVHPNKRRHGYATKQLQLALAEAQKLGLQKVLITCDKSNIGSAKTIQKVGGVLENEVVSSRT 159
Cdd:COG3981   80 LRHELNEFLLRVGGHIGYGVRPSERGKGYATEMLRLALEEARELGLDRVLITCDKDNIASRKVIEANGGVLEDEVVDEED 159

                        170
                 ....*....|.
gi 446693928 160 GEVIQRYWIEI 170
Cdd:COG3981  160 GRPVRRYWIDL 170
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 3-151 6.01e-13

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 63.09  E-value: 6.01e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928   3 LLKPIHEysEHITAYRQAFLHSGEQPHGSSSLQNFDSLDEWFEKvskqelgenlLANRVPSSQFLSF-----EKGKLIGF 77
Cdd:COG1670    9 RLRPLRP--EDAEALAELLNDPEVARYLPGPPYSLEEARAWLER----------LLADWADGGALPFaiedkEDGELIGV 76

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446693928  78 VNIRhrlNPELLRESGHIGYSVHPNKRRHGYATKQLQLALAEA-QKLGLQKVLITCDKSNIGSAKTIQKVGGVLE 151
Cdd:COG1670   77 VGLY---DIDRANRSAEIGYWLAPAYWGKGYATEALRALLDYAfEELGLHRVEAEVDPDNTASIRVLEKLGFRLE 148
Acetyltransf_3 pfam13302
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 38-147 1.15e-10

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 379112 [Multi-domain]  Cd Length: 139  Bit Score: 56.20  E-value: 1.15e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928   38 DSLDEWFEKVSKQelgenllANRVPSSQFLSFEKG-KLIGFVNIRHRLNPellRESGHIGYSVHPNKRRHGYATKQLQLA 116
Cdd:pfam13302  36 EEAREWLARIWAA-------DEAERGYGWAIELKDtGFIGSIGLYDIDGE---PERAELGYWLGPDYWGKGYATEAVRAL 105

                          90       100       110
                  ....*....|....*....|....*....|..
gi 446693928  117 LAEA-QKLGLQKVLITCDKSNIGSAKTIQKVG 147
Cdd:pfam13302 106 LEYAfEELGLPRLVARIDPENTASRRVLEKLG 137
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 66-147 4.20e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 51.75  E-value: 4.20e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928   66 FLSFEKGKLIGFVNIRHRLNPELLresGHI-GYSVHPNKRRHGYATKQLQLALAEAQKLGLQKVLITCDKSNIGSAKTIQ 144
Cdd:pfam00583  36 FVAEEDGELVGFASLSIIDDEPPV---GEIeGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLAAIALYE 112


                  ...
gi 446693928  145 KVG 147
Cdd:pfam00583 113 KLG 115
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 66-131 2.55e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 40.34  E-value: 2.55e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446693928  66 FLSFEKGKLIGFVNIRHRlnpELLRESGHIGY-SVHPNKRRHGYATKQLQLALAEAQKLGLQKVLIT 131
Cdd:cd04301    2 LVAEDDGEIVGFASLSPD---GSGGDTAYIGDlAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 76-147 3.62e-05

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 40.41  E-value: 3.62e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446693928  76 GFVNIRHRLNPEllreSGHIGY-SVHPNKRRHGYATKQLQLALAEAQKLGLQKVLITCDKSNIGSAKTIQKVG 147
Cdd:COG0456    1 GFALLGLVDGGD----EAEIEDlAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLG 69
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
70-139 3.83e-05

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 41.90  E-value: 3.83e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928  70 EKGKLIGFVNIRHRLNPELLRESGHIGYSVHPNKRRHGYATKQLQLALAEAQKLGLQKVLITCDKSNIGS 139
Cdd:COG1247   59 EDGEVVGFASLGPFRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEAS 128
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
99-147 1.76e-04

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 38.35  E-value: 1.76e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 446693928  99 VHPNKRRHGYATKQLQLALAEAQKLGLQKVLITCDKSNIGSAKTIQKVG 147
Cdd:COG3393   23 THPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLYERLG 71
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 62-128 5.36e-04

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 38.05  E-value: 5.36e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446693928  62 PSSQFLSFEK-GKLIGFVNIrHRLNPELlresGHIGY-SVHPNKRRHGYATKQLQLALAEAQKLGLQKV 128
Cdd:COG1246   26 EIGEFWVAEEdGEIVGCAAL-HPLDEDL----AELRSlAVHPDYRGRGIGRRLLEALLAEARELGLKRL 89
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 35-124 1.63e-03

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 36.78  E-value: 1.63e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446693928   35 QNFDSLDEWFEKVSKQELGENLLAnrvpssqflsFEKGKLIGFVNI-RHRLN-PELLRESGHIGY-SVHPNKRRHGYATK 111
Cdd:pfam13527  21 QDEDSPELREYFRPLLEEGRVLGA----------FDDGELVSTLALyPFELNvPGKTLPAAGITGvATYPEYRGRGVMSR 90

                          90
                  ....*....|...
gi 446693928  112 QLQLALAEAQKLG 124
Cdd:pfam13527  91 LLRRSLEEMRERG 103
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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