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Conserved domains on  [gi|446770911|ref|WP_000848167|]
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glycoside hydrolase family 99-like domain-containing protein [Bacillus thuringiensis]

Protein Classification

glycoside hydrolase family 99-like domain-containing protein( domain architecture ID 10184107)

glycoside hydrolase family 99-like domain-containing protein is typically found within O-antigen biosynthesis clusters and bears resemblance to the GH71/GH99 family of alpha-1,2-mannosidases

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Glyco_tran_WbsX cd11579
Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this ...
1-336 5.04e-178

Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this domain family are found in proteins within O-antigen biosynthesis clusters in Gram negative bacteria, where they may function as glycosyl hydrolases and typically co-occur with glycosyltransferase domains. They bear resemblance to GH71 and the GH99 family of alpha-1,2-mannosidases and may share a similar cataltyic site and mechanism. The O-antigens are essential lipopolysaccharides in gram-negative bacteria's outer membrane and have been linked to pathogenicity.


:

Pssm-ID: 211420 [Multi-domain]  Cd Length: 347  Bit Score: 496.67  E-value: 5.04e-178
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911   1 MKTIAFYLPQFHEIPENDLWWGKGFTEWTNVKKAKPRFLGHYQPRAPLRDYYYNLLNLDVHKWQINLAKQYGLDGFCYYH 80
Cdd:cd11579    1 MKVIAFYLPQFHPIPENDEWWGKGFTEWTNVKKAKPLFEGHYQPRVPLDLGYYDLRDPEVREKQAELAKEYGIDGFCYYH 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911  81 YWFKGKKLLEKPLELRNQCKEIDFPYCLCWANESWTRSWDGKDQEVLMRQEYGDESDWKEHFNYLLPFFKNKNYLNINDK 160
Cdd:cd11579   81 YWFGGKRLLEKPLEELLLSGEPDFPFCLCWANENWTRRWDGRDKEELLEQQYYGEEDWKEHFRYLLPYFKDPRYIKIDGK 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911 161 PIFVIYRPASIPNLNKMLGLWNKWAQYEGLLGIHFVEMLT-IFEDKSDFPFDAAIEFEPMYTLKKLIGNTNSLHQEKKDF 239
Cdd:cd11579  161 PVFLIYRPELIPDPKETLELWRELAREAGLPGLYLVAVASfGIEDPRDLGFDAAVEFPPHYVVASELSKSRKLLKLKKGF 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911 240 H---LSYDFVWKRILNRQIKErENIYKGAFVDWDNSPRKKESALIMEGASPDKFKKYL-------LQHSKDTDFLFINAW 309
Cdd:cd11579  241 GgnvYDYDDLVKYILNRPDPD-YPYYPGVFPGWDNTPRRGKRGTIFVNSTPELFKKWLrkaieraKARPEDERLVFINAW 319
                        330       340
                 ....*....|....*....|....*..
gi 446770911 310 NEWAEGTYLEPDEKYGYKYLEALMEIK 336
Cdd:cd11579  320 NEWAEGAYLEPDLKYGYGYLEALKEAL 346
 
Name Accession Description Interval E-value
Glyco_tran_WbsX cd11579
Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this ...
1-336 5.04e-178

Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this domain family are found in proteins within O-antigen biosynthesis clusters in Gram negative bacteria, where they may function as glycosyl hydrolases and typically co-occur with glycosyltransferase domains. They bear resemblance to GH71 and the GH99 family of alpha-1,2-mannosidases and may share a similar cataltyic site and mechanism. The O-antigens are essential lipopolysaccharides in gram-negative bacteria's outer membrane and have been linked to pathogenicity.


Pssm-ID: 211420 [Multi-domain]  Cd Length: 347  Bit Score: 496.67  E-value: 5.04e-178
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911   1 MKTIAFYLPQFHEIPENDLWWGKGFTEWTNVKKAKPRFLGHYQPRAPLRDYYYNLLNLDVHKWQINLAKQYGLDGFCYYH 80
Cdd:cd11579    1 MKVIAFYLPQFHPIPENDEWWGKGFTEWTNVKKAKPLFEGHYQPRVPLDLGYYDLRDPEVREKQAELAKEYGIDGFCYYH 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911  81 YWFKGKKLLEKPLELRNQCKEIDFPYCLCWANESWTRSWDGKDQEVLMRQEYGDESDWKEHFNYLLPFFKNKNYLNINDK 160
Cdd:cd11579   81 YWFGGKRLLEKPLEELLLSGEPDFPFCLCWANENWTRRWDGRDKEELLEQQYYGEEDWKEHFRYLLPYFKDPRYIKIDGK 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911 161 PIFVIYRPASIPNLNKMLGLWNKWAQYEGLLGIHFVEMLT-IFEDKSDFPFDAAIEFEPMYTLKKLIGNTNSLHQEKKDF 239
Cdd:cd11579  161 PVFLIYRPELIPDPKETLELWRELAREAGLPGLYLVAVASfGIEDPRDLGFDAAVEFPPHYVVASELSKSRKLLKLKKGF 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911 240 H---LSYDFVWKRILNRQIKErENIYKGAFVDWDNSPRKKESALIMEGASPDKFKKYL-------LQHSKDTDFLFINAW 309
Cdd:cd11579  241 GgnvYDYDDLVKYILNRPDPD-YPYYPGVFPGWDNTPRRGKRGTIFVNSTPELFKKWLrkaieraKARPEDERLVFINAW 319
                        330       340
                 ....*....|....*....|....*..
gi 446770911 310 NEWAEGTYLEPDEKYGYKYLEALMEIK 336
Cdd:cd11579  320 NEWAEGAYLEPDLKYGYGYLEALKEAL 346
Glyco_tran_WbsX pfam14307
Glycosyltransferase WbsX; Members of this family are found in within O-antigen biosynthesis ...
4-332 1.96e-167

Glycosyltransferase WbsX; Members of this family are found in within O-antigen biosynthesis clusters in Gram negative bacteria, where they are predicted to function as glycosyltransferases.


Pssm-ID: 464132 [Multi-domain]  Cd Length: 311  Bit Score: 468.52  E-value: 1.96e-167
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911    4 IAFYLPQFHEIPENDLWWGKGFTEWTNVKKAKPRFLGHYQPRAPLRDYYYNLLNLDVHKWQINLAKQYGLDGFCYYHYWF 83
Cdd:pfam14307   2 IAFYLPQFHPIPENDEWWGKGFTEWTNVKKAKPLFKGHYQPRLPADLGYYDLRLPEVRRAQAELAKEYGIYGFCYYHYWF 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911   84 KGKKLLEKPLE--LRNqcKEIDFPYCLCWANESWTRSWDGKDQEVLMRQEYGDEsDWKEHFNYLLPFFKNKNYLNINDKP 161
Cdd:pfam14307  82 GGKRLLEKPLEelLES--KEPDFPFCLCWANESWTRRWDGSDKEILIEQDYGEE-DDKAHFDYLLPYFKDPRYIKVDGKP 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911  162 IFVIYRPASIPNLNKMLGLWNKWAQYEGLLGIHFVEMLTIFEDKS-DFPFDAAIEFepmytlkkligntnslhqekkdfh 240
Cdd:pfam14307 159 LFLIYRPDLIPDPKETIERWRELAKENGLGGIYLVGVQSFGDEDPrDLGFDAAVEF------------------------ 214
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911  241 lSYDFVWKRILNRQIKErENIYKGAFVDWDNSPRKKESALIMEGASPDKFKKYL-------LQHSKDTDFLFINAWNEWA 313
Cdd:pfam14307 215 -DYDKLVRRILNRPPPK-YKLFPGVFPGWDNTPRRGKRGTIFHGATPEKFEKWLekaveraKQKPDEERIVFINAWNEWA 292
                         330
                  ....*....|....*....
gi 446770911  314 EGTYLEPDEKYGYKYLEAL 332
Cdd:pfam14307 293 EGAYLEPDLKFGYAYLEAI 311
RgpF COG3754
Lipopolysaccharide biosynthesis protein [Cell wall/membrane/envelope biogenesis];
1-95 2.28e-13

Lipopolysaccharide biosynthesis protein [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442968 [Multi-domain]  Cd Length: 152  Bit Score: 66.62  E-value: 2.28e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911   1 MKTIAFYLPQFHEI----------------------PENDLWWGKgfTEWTNvkkaKPRFLGHYQ-------PRA----P 47
Cdd:COG3754    4 VALFAFYLPQADELlltndsifgpiaplsplfdrmePENDDWWGL--GEWTN----KPLFPGHLQsyflvfrKRAlaspA 77
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 446770911  48 LRDYYYNLLNLD-----VHKWQINLAKQYGLDGFCYyHYWFKGKKLLEKPLEL 95
Cdd:COG3754   78 FRDFWYDLRLIEdkddvIRRYEAGLARALGIAGFCY-HYWFDGKRLRTRLLER 129
 
Name Accession Description Interval E-value
Glyco_tran_WbsX cd11579
Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this ...
1-336 5.04e-178

Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this domain family are found in proteins within O-antigen biosynthesis clusters in Gram negative bacteria, where they may function as glycosyl hydrolases and typically co-occur with glycosyltransferase domains. They bear resemblance to GH71 and the GH99 family of alpha-1,2-mannosidases and may share a similar cataltyic site and mechanism. The O-antigens are essential lipopolysaccharides in gram-negative bacteria's outer membrane and have been linked to pathogenicity.


Pssm-ID: 211420 [Multi-domain]  Cd Length: 347  Bit Score: 496.67  E-value: 5.04e-178
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911   1 MKTIAFYLPQFHEIPENDLWWGKGFTEWTNVKKAKPRFLGHYQPRAPLRDYYYNLLNLDVHKWQINLAKQYGLDGFCYYH 80
Cdd:cd11579    1 MKVIAFYLPQFHPIPENDEWWGKGFTEWTNVKKAKPLFEGHYQPRVPLDLGYYDLRDPEVREKQAELAKEYGIDGFCYYH 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911  81 YWFKGKKLLEKPLELRNQCKEIDFPYCLCWANESWTRSWDGKDQEVLMRQEYGDESDWKEHFNYLLPFFKNKNYLNINDK 160
Cdd:cd11579   81 YWFGGKRLLEKPLEELLLSGEPDFPFCLCWANENWTRRWDGRDKEELLEQQYYGEEDWKEHFRYLLPYFKDPRYIKIDGK 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911 161 PIFVIYRPASIPNLNKMLGLWNKWAQYEGLLGIHFVEMLT-IFEDKSDFPFDAAIEFEPMYTLKKLIGNTNSLHQEKKDF 239
Cdd:cd11579  161 PVFLIYRPELIPDPKETLELWRELAREAGLPGLYLVAVASfGIEDPRDLGFDAAVEFPPHYVVASELSKSRKLLKLKKGF 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911 240 H---LSYDFVWKRILNRQIKErENIYKGAFVDWDNSPRKKESALIMEGASPDKFKKYL-------LQHSKDTDFLFINAW 309
Cdd:cd11579  241 GgnvYDYDDLVKYILNRPDPD-YPYYPGVFPGWDNTPRRGKRGTIFVNSTPELFKKWLrkaieraKARPEDERLVFINAW 319
                        330       340
                 ....*....|....*....|....*..
gi 446770911 310 NEWAEGTYLEPDEKYGYKYLEALMEIK 336
Cdd:cd11579  320 NEWAEGAYLEPDLKYGYGYLEALKEAL 346
Glyco_tran_WbsX pfam14307
Glycosyltransferase WbsX; Members of this family are found in within O-antigen biosynthesis ...
4-332 1.96e-167

Glycosyltransferase WbsX; Members of this family are found in within O-antigen biosynthesis clusters in Gram negative bacteria, where they are predicted to function as glycosyltransferases.


Pssm-ID: 464132 [Multi-domain]  Cd Length: 311  Bit Score: 468.52  E-value: 1.96e-167
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911    4 IAFYLPQFHEIPENDLWWGKGFTEWTNVKKAKPRFLGHYQPRAPLRDYYYNLLNLDVHKWQINLAKQYGLDGFCYYHYWF 83
Cdd:pfam14307   2 IAFYLPQFHPIPENDEWWGKGFTEWTNVKKAKPLFKGHYQPRLPADLGYYDLRLPEVRRAQAELAKEYGIYGFCYYHYWF 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911   84 KGKKLLEKPLE--LRNqcKEIDFPYCLCWANESWTRSWDGKDQEVLMRQEYGDEsDWKEHFNYLLPFFKNKNYLNINDKP 161
Cdd:pfam14307  82 GGKRLLEKPLEelLES--KEPDFPFCLCWANESWTRRWDGSDKEILIEQDYGEE-DDKAHFDYLLPYFKDPRYIKVDGKP 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911  162 IFVIYRPASIPNLNKMLGLWNKWAQYEGLLGIHFVEMLTIFEDKS-DFPFDAAIEFepmytlkkligntnslhqekkdfh 240
Cdd:pfam14307 159 LFLIYRPDLIPDPKETIERWRELAKENGLGGIYLVGVQSFGDEDPrDLGFDAAVEF------------------------ 214
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911  241 lSYDFVWKRILNRQIKErENIYKGAFVDWDNSPRKKESALIMEGASPDKFKKYL-------LQHSKDTDFLFINAWNEWA 313
Cdd:pfam14307 215 -DYDKLVRRILNRPPPK-YKLFPGVFPGWDNTPRRGKRGTIFHGATPEKFEKWLekaveraKQKPDEERIVFINAWNEWA 292
                         330
                  ....*....|....*....
gi 446770911  314 EGTYLEPDEKYGYKYLEAL 332
Cdd:pfam14307 293 EGAYLEPDLKFGYAYLEAI 311
GH99_GH71_like cd11573
Glycoside hydrolase families 71, 99, and related domains; This superfamily of glycoside ...
38-326 1.30e-20

Glycoside hydrolase families 71, 99, and related domains; This superfamily of glycoside hydrolases contains families GH71 and GH99 (following the CAZY nomenclature), as well as other members with undefined function and specificity.


Pssm-ID: 211414  Cd Length: 284  Bit Score: 89.86  E-value: 1.30e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911  38 FLGHYQPRAPlrdyyynllnlDVHKWQINLAKQYGLDGFCYYHYWFKG-------KKLLEKPLElRNQCKEIDFPYCLCW 110
Cdd:cd11573    1 VFAGYQPWTP-----------EVMRKHIRWAQEAGIDGFAVDWYPEADtsplaetTAILNKALD-AAEEENFTIFFMLDP 68
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911 111 ANESWTRSWDgkdqevlmrqeygdesDWKEHFNYLLPFFKN-KNYLNINDKPIFVIYRPAsipnLNKMLGLWNKWAQYEG 189
Cdd:cd11573   69 ASLREAGELD----------------VVLERITRLINEYRNpSSYYKVGGKPLVFIWGPG----LAYTASEWEALKAQLR 128
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911 190 LLGIHFVEMLTIFEDKSdfPFDAAIEFEPMYTLKKLIGNTNSLHQEKKDFHLSYDFVWKrilnrqiKERENIY-KGAFVD 268
Cdd:cd11573  129 AGCPYMIGLWTPWRVPN--RDMITDMFDGASPWTPWRGTNPEEAYGHGVKNWRPDQEWM-------GANGKGYiPTVSPG 199
                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911 269 W-DNSPRKKESALIMEGASPDKFKKYLLQHSKDT-DFLFINAWNEWAEGTYLEPDEKYGY 326
Cdd:cd11573  200 FsDINRRPGDPGDIILRRDGQRLHSMLEAALKAGpAMIQIASWNDWGEGTYIEPCEEYGP 259
RgpF COG3754
Lipopolysaccharide biosynthesis protein [Cell wall/membrane/envelope biogenesis];
1-95 2.28e-13

Lipopolysaccharide biosynthesis protein [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442968 [Multi-domain]  Cd Length: 152  Bit Score: 66.62  E-value: 2.28e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446770911   1 MKTIAFYLPQFHEI----------------------PENDLWWGKgfTEWTNvkkaKPRFLGHYQ-------PRA----P 47
Cdd:COG3754    4 VALFAFYLPQADELlltndsifgpiaplsplfdrmePENDDWWGL--GEWTN----KPLFPGHLQsyflvfrKRAlaspA 77
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 446770911  48 LRDYYYNLLNLD-----VHKWQINLAKQYGLDGFCYyHYWFKGKKLLEKPLEL 95
Cdd:COG3754   78 FRDFWYDLRLIEdkddvIRRYEAGLARALGIAGFCY-HYWFDGKRLRTRLLER 129
GH99_GH71_like_1 cd11578
Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside ...
286-335 1.04e-04

Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.


Pssm-ID: 211419  Cd Length: 313  Bit Score: 43.55  E-value: 1.04e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 446770911 286 SPDKFKKYL---LQHSKDTDFLFINAWNEWAEGTYLEP-DEKYGYKYLEALMEI 335
Cdd:cd11578  255 NPSSFKKMCnvaLRNDGACNIVLITSFNEWNEGTNIEPsEEAYGFGYLETLLDE 308
GH99_GH71_like_3 cd11575
Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside ...
302-334 1.87e-04

Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.


Pssm-ID: 211416  Cd Length: 376  Bit Score: 42.71  E-value: 1.87e-04
                         10        20        30
                 ....*....|....*....|....*....|...
gi 446770911 302 DFLFINAWNEWAEGTYLEPDEKYGYKYLEALME 334
Cdd:cd11575  291 DIIQIATWNDYGEGTMIEPTVEFGYRDLETTQQ 323
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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