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Conserved domains on  [gi|446855091|ref|WP_000932347|]
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MULTISPECIES: tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL [Enterobacteriaceae]

Protein Classification

tRNA (cytidine(34)-2'-O)-methyltransferase( domain architecture ID 10013498)

tRNA (cytidine(34)-2'-O)-methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine to the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-157 4.26e-125

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


:

Pssm-ID: 182406  Cd Length: 157  Bit Score: 347.37  E-value: 4.26e-125
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   1 MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTT 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446855091  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD 157
Cdd:PRK10358  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD 157
 
Name Accession Description Interval E-value
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-157 4.26e-125

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 347.37  E-value: 4.26e-125
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   1 MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTT 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446855091  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD 157
Cdd:PRK10358  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD 157
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-154 3.60e-106

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 299.26  E-value: 3.60e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   1 MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTT 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446855091  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDAlPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAV 154
Cdd:COG0219   81 KGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGLV 153
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-154 2.91e-102

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 289.70  E-value: 2.91e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091    1 MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTT 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQKLFALTK 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446855091   81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALpAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAV 154
Cdd:TIGR00185  81 KGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNL-MEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGLV 153
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 3-148 1.24e-93

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 267.40  E-value: 1.24e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   3 NIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKG 82
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGRLFLFTTKG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446855091  83 TPAHSAVSYQDGDYLMFGPETRGLPASILDALPaEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQL 148
Cdd:cd18094   81 TKPYTDFDFKPGDYLVFGRESTGLPEEILDAFP-DRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-142 2.03e-43

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 140.36  E-value: 2.03e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091    1 MLNIVLYEPEIPPNTGNIIRLCANTGF-RLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQ--RLFA 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGAdGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAgyAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446855091   78 LTTKGTPAHsAVSYQDGDYLMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVY 142
Cdd:pfam00588  81 TSLGGKSLY-EIDFPKKVALVFGNEGTGLSNEVLEL--CDRLVRIPMSGDVESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-157 4.26e-125

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 347.37  E-value: 4.26e-125
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   1 MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTT 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446855091  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD 157
Cdd:PRK10358  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD 157
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-154 3.60e-106

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 299.26  E-value: 3.60e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   1 MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTT 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446855091  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDAlPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAV 154
Cdd:COG0219   81 KGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGLV 153
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-154 2.91e-102

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 289.70  E-value: 2.91e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091    1 MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTT 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQKLFALTK 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446855091   81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALpAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAV 154
Cdd:TIGR00185  81 KGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNL-MEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGLV 153
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 3-148 1.24e-93

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 267.40  E-value: 1.24e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   3 NIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKG 82
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGRLFLFTTKG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446855091  83 TPAHSAVSYQDGDYLMFGPETRGLPASILDALPaEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQL 148
Cdd:cd18094   81 TKPYTDFDFKPGDYLVFGRESTGLPEEILDAFP-DRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-142 2.03e-43

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 140.36  E-value: 2.03e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091    1 MLNIVLYEPEIPPNTGNIIRLCANTGF-RLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQ--RLFA 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGAdGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAgyAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446855091   78 LTTKGTPAHsAVSYQDGDYLMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVY 142
Cdd:pfam00588  81 TSLGGKSLY-EIDFPKKVALVFGNEGTGLSNEVLEL--CDRLVRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-144 5.10e-29

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 103.84  E-value: 5.10e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   4 IVLYEPEIPPNTGNIIRLCANTGFR-LHIIEPMGFAWDDKrLRRAGLDYHEFTAVTRHHDYRAFLEAENPQ--RLFALTT 80
Cdd:cd18082    2 LVLDNIRSPGNIGSIFRTADAFGVEkIYLVGPTADIFDPK-AKRTSRGTHFSVPYERVDNFEEYLKSLKEEgyPLIGLEL 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446855091  81 KGT---PAHSAVSYQDGDYLMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVYEA 144
Cdd:cd18082   81 TDGsipLSEFKFKKPKKIALIFGNERSGLSPELLAL--CDYTVHIPMSGKNSSLNVSVATGIALYEF 145
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 2-143 1.59e-13

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 63.67  E-value: 1.59e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   2 LNIVLYEPEIPPNTGNIIRLCANTGF-RLHIIEPMGFAWDDKRLRRA--GLDYHE--------------------FTAVT 58
Cdd:cd18093    1 IRVVLVEPQHPGNIGAVARAMKNFGLsDLRLVNPRCDDPEEAARALAsgAEDVLEnakvydtleealadcdlvvaTTARP 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091  59 RHHDYRAFLEAENPQRLFALTTKGTPAhsavsyqdgdyLMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVS 138
Cdd:cd18093   81 RGLRRPLLTPRELAELLKEHAAGGKVA-----------LVFGREDSGLTNEELAL--CDLLVTIPTNPEYPSLNLAQAVA 147


                 ....*
gi 446855091 139 VVVYE 143
Cdd:cd18093  148 IVLYE 152
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 14-148 1.19e-11

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 59.02  E-value: 1.19e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091  14 NTGNIIRLCANTGF-RLHIIEPMGfawDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAenpqrlfaLTTKG------TPAH 86
Cdd:cd18092   29 NASAVLRTCDAFGVqDVHVIEPKN---KFKKNRGVAMGADKWLDVHRHDSTEECLAA--------LKEQGyrivatSLSP 97

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446855091  87 SAVSYQDGDY-----LMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQL 148
Cdd:cd18092   98 DAVPLDELDYtrptaLVFGNEKDGVSEEALAA--ADERVYIPMYGFVESLNVSVAAAIILYELQRQR 162
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 4-149 2.41e-11

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 59.36  E-value: 2.41e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   4 IVLYEPEIPPNTGNIIRLCANTGF-RLHIIEPMGFAwDDKRLRRAG-----LD---YHE--------------FTAVTRH 60
Cdd:COG0565    6 VVLVEPQHPGNIGAAARAMKNFGLsDLRLVNPRGWP-DEEARAMASgaddvLEnarVVDtleealadcdlvvgTTARPRE 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091  61 HDYRAFLEAENPQRLFALTTKGTPAhsavsyqdgdyLMFGPETRGLPASIL---DALpaeqkIRIPMVPDSRSMNLSNAV 137
Cdd:COG0565   85 LRKPVLTPREAAEELLARAAGGKVA-----------LVFGRERSGLTNEELalcDAL-----VTIPTNPEYPSLNLAQAV 148

                        170
                 ....*....|..
gi 446855091 138 SVVVYEAWRQLG 149
Cdd:COG0565  149 QVVAYELRKAAL 160
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 82-147 1.40e-10

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 57.51  E-value: 1.40e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446855091  82 GTPAHSAVSYQDGDY-----LMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQ 147
Cdd:COG0566  193 GTDLDGEASLYEVDLpgpvaLVLGNEGKGLSRLVLEA--CDELVRIPMAGGVESLNVAVAAAILLYEAARQ 261
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 86-147 5.96e-10

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 54.12  E-value: 5.96e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446855091  86 HSAVSYQDGDY-----LMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQ 147
Cdd:cd18104   81 KAEKLYYEADLrgpiaIVVGSESEGLSEFWLKA--ADERVRIPMAGQADSLNVSVAAAILLYEALRQ 145
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 4-144 1.36e-08

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 50.85  E-value: 1.36e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   4 IVLYE-PEIPPNTGNIIRLCAntGFRLHIIEPMGFAWD--DKRLRRAGLDYHEFTAVTR---HHDYRAFLEAENPqRLFA 77
Cdd:cd18107    2 VVLFDrPTSPGNLGTIIRSAD--ALGAHGLVVTGHAADvyDPKTIRASTGSLFSLPIVRvpsNRELEDWVEARKA-RGTP 78

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446855091  78 LTTKGTPAHSAVSYQDGDY-----LMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVYEA 144
Cdd:cd18107   79 LQLVGTDEHGDHQIADHDFtrptlLLIGNETAGLSAAWREL--CDETVSIPMAGSASSLNAACAASIFLYEA 148
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 12-144 1.92e-07

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 47.42  E-value: 1.92e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091  12 PPNTGNIIRLCANTGFRlHIIEPMG--FAWDDKRLRRA-GldYHEFTAVTRHHDYRAFLEA--ENPQRLFALTTKGTPAH 86
Cdd:cd18095   11 PGNLGTIIRTAAAFGVD-GVILSKGtvDPYNPKVVRASmG--ALFRLPIIRVDDLEELLEElkEAGYKVVAADLDGGSSL 87

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 446855091  87 SAVSYQDGDYLMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVYEA 144
Cdd:cd18095   88 YDLDLPKPVALVLGNEGNGLSDELLEL--CDKRVTIPMSGGVESLNVAVAAAILLYEL 143
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 14-144 2.44e-07

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 47.17  E-value: 2.44e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091  14 NTGNIIRLCANTGF-RLHI----IEPmgfawDDKRLRRAGLDYHEFTAVTRHHDYRAFLEA--ENPQRLFALTTkgtpAH 86
Cdd:cd18097   13 NVGSIFRTADAAGVeKIYLcgytPTP-----PHPKVAKTALGAEESVPWEHFEDTLEAVRAlkARGYTIIALET----TE 83

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446855091  87 SAVSYQDGDY-----LMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVYEA 144
Cdd:cd18097   84 NSVPLHEARLprplaLVLGNEVRGVSPEVLAL--CDAVVEIPMFGRKNSLNVAVAAAVALYEL 144
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 1-143 1.16e-06

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 46.24  E-value: 1.16e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091    1 MLNIVLYEPEIPPNTGNIIRLCANTGFR-LHIIEPMGFAWDDKRLRRAgldyHEFTAVTRHHDYRAFLEA-ENPQRLFAL 78
Cdd:TIGR00050   3 NIRIVLVEPSHPGNIGSIARAMKNMGLTeLRLVNPRVKLEEEAYALAA----GARDILDNAKVVDTLDEAlDDCDFVVGT 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446855091   79 TTKG--------TP---AHSAVSYQDGDYLMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVYE 143
Cdd:TIGR00050  79 SARSrnlqrpmlTPrelAPKLVAEKGKVALVFGREDSGLTNEELQK--CHVLVSIPTSEEYPSLNLSHAVAVILYE 152
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 87-147 2.67e-06

tRNA guanosine-2'-O-methyltransferase; Provisional


Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 45.37  E-value: 2.67e-06
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446855091  87 SAVSYQDGDY-----LMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQ 147
Cdd:PRK11081 101 TAVDFREIDYtrptcILMGQEKTGISQEALAL--ADQDIIIPMIGMVQSLNVSVASALILYEAQRQ 164
SpoU-like cd18098
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-142 2.12e-05

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349971  Cd Length: 138  Bit Score: 41.86  E-value: 2.12e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091   4 IVLYEPEIPPNTGNIIRLCANTGFrlhiiePMGFAWDDKRLRRAG-------------LDYHEFTAVTRHHDYRAFLEAe 70
Cdd:cd18098    3 IGIENPKTPKNVGTLWRAAGCLGA------SFVFTIGARYKRRKQstdtskawrhiplFHYDDLEDLAEPLPFGAVLVA- 75

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446855091  71 npqrlFALTTKGTPAHSAVSYQDGDYLmFGPETRGLPASILDALPAeqkirIPMVPDSRSMNLSNAVSVVVY 142
Cdd:cd18098   76 -----VELTEGATPLPDFKHPDRAVYL-LGPEDGGLPQEVLARCHH-----VVYLPTRGSLNVAVAGSIVLY 136
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 82-144 2.96e-05

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 41.60  E-value: 2.96e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446855091  82 GTPAHSAVSYQDGDY-----LMFGPETRGLPASILDAlpAEQKIRIPMVPDSRSMNLSNAVSVVVYEA 144
Cdd:cd18103   78 GADGEAEKSLYDVDLtgplaLVLGSEGKGLRRLVLKN--CDFLVKIPMAGKVDSLNVSVAAGIILYEI 143
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 12-144 1.09e-04

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 39.93  E-value: 1.09e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091  12 PPNTGNIIRLCANTGFRlHIIEPMGFA--WDDKRLRrAGLDYHeFtavtRHHDYRAfLEAENPQRLFALTTKGTPAHS-- 87
Cdd:cd18106   11 PGNLGSILRSAAAAGCS-KVLLTKGCVdpWEPKVLR-AGAGAH-F----RLPIYGN-LSWDDIPNYLPDDSKVFLADNnl 82

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446855091  88 -AVSYQDGDY------LMFGPETRGLPASILDALPAEQKIR--IPMVPDSRSMNLSNAVSVVVYEA 144
Cdd:cd18106   83 pVQPYDSIDYcsgpivLVIGGETHGVSPEAYKLAHSTGGSRvhIPLANGVDSLNSAVAASILLFEM 148
SpoU-like_NHR cd18108
Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) ...
 41-143 2.54e-03

Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) confers resistance to the thiazole antibiotic nosiheptide via catalyzing 2'O-methylation of 23S rRNA at the nucleotide A1067. NHR is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349981  Cd Length: 144  Bit Score: 36.30  E-value: 2.54e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446855091  41 DKRLRRA--GLDYHEFTAVTRHHDYRAFLEaENPQRLFALTTKG-TPAHSAVSYQDGDYLMFGPETRGlPASILDALpAE 117
Cdd:cd18108   41 DRRLLRAsrGYVFSLPVVLADREEAVAFLR-DNDIPLMTLDADGdVPVKELGDRPDRLALVFGSEKGG-PSGLFEEA-AA 117

                         90       100
                 ....*....|....*....|....*.
gi 446855091 118 QKIRIPMVPDSRSMNLSNAVSVVVYE 143
Cdd:cd18108  118 GTVSIPMSSSTESLNVSVSAGIALHE 143
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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