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Conserved domains on  [gi|446862764|ref|WP_000940020|]
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MULTISPECIES: tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ [Enterobacteriaceae]

Protein Classification

tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ( domain architecture ID 11487661)

tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK15114 PRK15114
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional
 1-244 0e+00

tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional


:

Pssm-ID: 185069  Cd Length: 245  Bit Score: 522.39  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS 80
Cdd:PRK15114   1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNATIVDTLDEALAGCSLVVGTS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  81 ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160
Cdd:PRK15114  81 ARSRTLPWPMLDPRECGLKSVAEAANAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764 161 LATQENGE-QVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQ 239
Cdd:PRK15114 161 LATQENGEpQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQ 240


                 ....*
gi 446862764 240 NKGNK 244
Cdd:PRK15114 241 NKGNK 245
 
Name Accession Description Interval E-value
PRK15114 PRK15114
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional
 1-244 0e+00

tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional


Pssm-ID: 185069  Cd Length: 245  Bit Score: 522.39  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS 80
Cdd:PRK15114   1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNATIVDTLDEALAGCSLVVGTS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  81 ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160
Cdd:PRK15114  81 ARSRTLPWPMLDPRECGLKSVAEAANAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764 161 LATQENGE-QVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQ 239
Cdd:PRK15114 161 LATQENGEpQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQ 240


                 ....*
gi 446862764 240 NKGNK 244
Cdd:PRK15114 241 NKGNK 245
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 2-241 5.79e-133

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 374.07  E-value: 5.79e-133
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   2 LQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSA 81
Cdd:COG0565    1 LDNIRVVLVEPQHPGNIGAAARAMKNFGLSDLRLVNPRGWPDEEARAMASGADDVLENARVVDTLEEALADCDLVVGTTA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  82 RSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWL 161
Cdd:COG0565   81 RPRELRKPVLTPREAAEELLARAAGGKVALVFGRERSGLTNEELALCDALVTIPTNPEYPSLNLAQAVQVVAYELRKAAL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764 162 ATQengEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQNK 241
Cdd:COG0565  161 EAP---PLPPPPEPEPATAEELEGLYEHLEELLDAIGFLDPEKPEKLMRRLRRLFGRARLTEREVRTLRGILRALERRLK 237
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 2-241 1.68e-126

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 357.86  E-value: 1.68e-126
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764    2 LQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSA 81
Cdd:TIGR00050   1 LENIRIVLVEPSHPGNIGSIARAMKNMGLTELRLVNPRVKLEEEAYALAAGARDILDNAKVVDTLDEALDDCDFVVGTSA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   82 RSRTLPWPMLDPRECGLKSVAEAAntPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWL 161
Cdd:TIGR00050  81 RSRNLQRPMLTPRELAPKLVAEKG--KVALVFGREDSGLTNEELQKCHVLVSIPTSEEYPSLNLSHAVAVILYELRMAWL 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  162 ATQENgeqveHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQNK 241
Cdd:TIGR00050 159 AQQEN-----LSEMPYATVDDLELLLEHLEETLLSLGFIPEHKPRQVMRKFRRLYGRAGLEKRELHILRGILRSIEKRIK 233
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 5-155 6.13e-77

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 229.30  E-value: 6.13e-77
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   5 IRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQ-AIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARS 83
Cdd:cd18093    1 IRVVLVEPQHPGNIGAVARAMKNFGLSDLRLVNPRCDDPEEaARALASGAEDVLENAKVYDTLEEALADCDLVVATTARP 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446862764  84 RTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYE 155
Cdd:cd18093   81 RGLRRPLLTPRELAELLKEHAAGGKVALVFGREDSGLTNEELALCDLLVTIPTNPEYPSLNLAQAVAIVLYE 152
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 4-154 1.13e-32

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 116.09  E-value: 1.13e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764    4 NIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKP--DSQAIALAAGASDVIgNAHIVDTLDEALAGCSL----VV 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGADGLILVEPRSADpyNPKAIRASAGALFHL-PLVVVDNLEEALKDLKEagyaVV 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446862764   78 GTSARSRTLPwpmlDPRECGlksvaeaantPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAY 154
Cdd:pfam00588  80 ATSLGGKSLY----EIDFPK----------KVALVFGNEGTGLSNEVLELCDRLVRIPMSGDVESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
PRK15114 PRK15114
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional
 1-244 0e+00

tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional


Pssm-ID: 185069  Cd Length: 245  Bit Score: 522.39  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS 80
Cdd:PRK15114   1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNATIVDTLDEALAGCSLVVGTS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  81 ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160
Cdd:PRK15114  81 ARSRTLPWPMLDPRECGLKSVAEAANAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764 161 LATQENGE-QVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQ 239
Cdd:PRK15114 161 LATQENGEpQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQ 240


                 ....*
gi 446862764 240 NKGNK 244
Cdd:PRK15114 241 NKGNK 245
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 2-241 5.79e-133

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 374.07  E-value: 5.79e-133
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   2 LQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSA 81
Cdd:COG0565    1 LDNIRVVLVEPQHPGNIGAAARAMKNFGLSDLRLVNPRGWPDEEARAMASGADDVLENARVVDTLEEALADCDLVVGTTA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  82 RSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWL 161
Cdd:COG0565   81 RPRELRKPVLTPREAAEELLARAAGGKVALVFGRERSGLTNEELALCDALVTIPTNPEYPSLNLAQAVQVVAYELRKAAL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764 162 ATQengEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQNK 241
Cdd:COG0565  161 EAP---PLPPPPEPEPATAEELEGLYEHLEELLDAIGFLDPEKPEKLMRRLRRLFGRARLTEREVRTLRGILRALERRLK 237
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 2-241 1.68e-126

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 357.86  E-value: 1.68e-126
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764    2 LQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSA 81
Cdd:TIGR00050   1 LENIRIVLVEPSHPGNIGSIARAMKNMGLTELRLVNPRVKLEEEAYALAAGARDILDNAKVVDTLDEALDDCDFVVGTSA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   82 RSRTLPWPMLDPRECGLKSVAEAAntPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWL 161
Cdd:TIGR00050  81 RSRNLQRPMLTPRELAPKLVAEKG--KVALVFGREDSGLTNEELQKCHVLVSIPTSEEYPSLNLSHAVAVILYELRMAWL 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  162 ATQENgeqveHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQNK 241
Cdd:TIGR00050 159 AQQEN-----LSEMPYATVDDLELLLEHLEETLLSLGFIPEHKPRQVMRKFRRLYGRAGLEKRELHILRGILRSIEKRIK 233
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 5-155 6.13e-77

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 229.30  E-value: 6.13e-77
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   5 IRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQ-AIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARS 83
Cdd:cd18093    1 IRVVLVEPQHPGNIGAVARAMKNFGLSDLRLVNPRCDDPEEaARALASGAEDVLENAKVYDTLEEALADCDLVVATTARP 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446862764  84 RTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYE 155
Cdd:cd18093   81 RGLRRPLLTPRELAELLKEHAAGGKVALVFGREDSGLTNEELALCDLLVTIPTNPEYPSLNLAQAVAIVLYE 152
PRK10433 PRK10433
putative RNA methyltransferase; Provisional
 5-157 9.76e-38

putative RNA methyltransferase; Provisional


Pssm-ID: 236693  Cd Length: 228  Bit Score: 131.65  E-value: 9.76e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   5 IRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSR 84
Cdd:PRK10433   3 LTIILVAPARAENVGAAARAMKTMGFSELRIVDSQAHLEPAARWVAHGSGDILDNAKVFDTLAEALHDVDFTVATTARSR 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446862764  85 TLPWPMLDPRECgLKSVAEAAN--TPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVR 157
Cdd:PRK10433  83 AKFHYYATPAEL-VPLLEEKSSwmSHAALVFGREDSGLTNEELALADVLTGVPMAADYPSLNLGQAVMVYCYQLA 156
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 4-154 1.13e-32

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 116.09  E-value: 1.13e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764    4 NIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKP--DSQAIALAAGASDVIgNAHIVDTLDEALAGCSL----VV 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGADGLILVEPRSADpyNPKAIRASAGALFHL-PLVVVDNLEEALKDLKEagyaVV 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446862764   78 GTSARSRTLPwpmlDPRECGlksvaeaantPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAY 154
Cdd:pfam00588  80 ATSLGGKSLY----EIDFPK----------KVALVFGNEGTGLSNEVLELCDRLVRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 6-155 1.99e-27

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 102.30  E-value: 1.99e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   6 RIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALA-----GCsLVVGTs 80
Cdd:cd18082    1 HLVLDNIRSPGNIGSIFRTADAFGVEKIYLVGPTADIFDPKAKRTSRGTHFSVPYERVDNFEEYLKslkeeGY-PLIGL- 78

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446862764  81 arsrtlpwpMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYE 155
Cdd:cd18082   79 ---------ELTDGSIPLSEFKFKKPKKIALIFGNERSGLSPELLALCDYTVHIPMSGKNSSLNVSVATGIALYE 144
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 16-155 3.87e-09

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 53.58  E-value: 3.87e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  16 GNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAA-GAS---DVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPML 91
Cdd:cd18095   12 GNLGTIIRTAAAFGVDGVILSKGTVDPYNPKVVRASmGALfrlPIIRVDDLEELLEELKEAGYKVVAADLDGGSSLYDLD 91

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446862764  92 DPRecglksvaeaantPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYE 155
Cdd:cd18095   92 LPK-------------PVALVLGNEGNGLSDELLELCDKRVTIPMSGGVESLNVAVAAAILLYE 142
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 3-158 5.42e-09

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 53.63  E-value: 5.42e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   3 QNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLV-NPLVKPDSQAIAlaAGASDVIgNAHIVDTLDEALA-----GCSLV 76
Cdd:cd18092   15 RDLTVVLENVHDPHNASAVLRTCDAFGVQDVHVIePKNKFKKNRGVA--MGADKWL-DVHRHDSTEECLAalkeqGYRIV 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  77 VgTSARSRTLPwpmldPRECGLKsvaeaanTPVALVFGRERVGLTNEELQKCHYHVAIaanPEY---SSLNLAMAVQVIA 153
Cdd:cd18092   92 A-TSLSPDAVP-----LDELDYT-------RPTALVFGNEKDGVSEEALAAADERVYI---PMYgfvESLNVSVAAAIIL 155


                 ....*
gi 446862764 154 YEVRM 158
Cdd:cd18092  156 YELQR 160
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 1-156 5.28e-07

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 47.56  E-value: 5.28e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   1 MLQNIRivlvetsHTGNMGSVARAMKTMGLTNLWLV-NPLVKPDSQAIALAAGASDVIGNAHIVDTLDealagcsLVVGT 79
Cdd:cd18097    4 VLDNLR-------SAHNVGSIFRTADAAGVEKIYLCgYTPTPPHPKVAKTALGAEESVPWEHFEDTLE-------AVRAL 69

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  80 SARSRTLpwpmldpreCGLKSVAEA-------ANTPVALVFGRERVGLTNEELQKCHYHVAIaanPEY---SSLNLAMAV 149
Cdd:cd18097   70 KARGYTI---------IALETTENSvplhearLPRPLALVLGNEVRGVSPEVLALCDAVVEI---PMFgrkNSLNVAVAA 137


                 ....*..
gi 446862764 150 QVIAYEV 156
Cdd:cd18097  138 AVALYEL 144
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 108-157 3.76e-06

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 46.73  E-value: 3.76e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 446862764 108 PVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVR 157
Cdd:COG0566  210 PVALVLGNEGKGLSRLVLEACDELVRIPMAGGVESLNVAVAAAILLYEAA 259
SpoU-like cd18098
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 112-154 7.40e-06

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349971  Cd Length: 138  Bit Score: 44.56  E-value: 7.40e-06
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 446862764 112 VFGRERVGLTNEELQKCHYHVAIaanPEYSSLNLAMAVQVIAY 154
Cdd:cd18098   97 LLGPEDGGLPQEVLARCHHVVYL---PTRGSLNVAVAGSIVLY 136
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 108-158 1.18e-05

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 44.11  E-value: 1.18e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 446862764 108 PVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRM 158
Cdd:cd18104   94 PIAIVVGSESEGLSEFWLKAADERVRIPMAGQADSLNVSVAAAILLYEALR 144
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 106-156 1.53e-05

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 43.53  E-value: 1.53e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 446862764 106 NTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEV 156
Cdd:cd18103   93 TGPLALVLGSEGKGLRRLVLKNCDFLVKIPMAGKVDSLNVSVAAGIILYEI 143
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 110-160 2.77e-05

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 43.10  E-value: 2.77e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 446862764 110 ALVFGRERVGLTNEELQK-CHYHVAIAANPEYSSLNLAMAVQVIAYEvrmAW 160
Cdd:COG0219   96 VLLFGRETAGLPEEVLAApADQRLRIPMRPGVRSLNLSNAVAIVLYE---AL 144
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 111-156 3.67e-05

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 42.44  E-value: 3.67e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 446862764 111 LVFGRERVGLTNEELQKC-HYHVAIAANPEYSSLNLAMAVQVIAYEV 156
Cdd:cd18094   95 LVFGRESTGLPEEILDAFpDRRLRIPMRPKVRSLNLSNSVAIVLYEA 141
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 16-155 5.68e-05

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 41.85  E-value: 5.68e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  16 GNMGSVARAMKTMGLTNLWLVNPLVKP-DSQAIALAAGAS---DVIGNAHIvDTLDEALAGCSLVVGTSARSRTLPWPML 91
Cdd:cd18106   12 GNLGSILRSAAAAGCSKVLLTKGCVDPwEPKVLRAGAGAHfrlPIYGNLSW-DDIPNYLPDDSKVFLADNNLPVQPYDSI 90

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446862764  92 DprecglksvaeAANTPVALVFGRERVGLTNEELQKCH----YHVAIAANPEYSSLNLAMAVQVIAYE 155
Cdd:cd18106   91 D-----------YCSGPIVLVIGGETHGVSPEAYKLAHstggSRVHIPLANGVDSLNSAVAASILLFE 147
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 13-156 2.53e-04

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 40.06  E-value: 2.53e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  13 SHTGNMGSVARAmktmgltnlwlvnplvkpdsqaiALAAGASDVIGNAHIVDTLDEALAGCSL-------VVGTSARSRT 85
Cdd:cd18107    9 TSPGNLGTIIRS-----------------------ADALGAHGLVVTGHAADVYDPKTIRASTgslfslpIVRVPSNREL 65

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  86 LPW-PMLDPRECGLKSVA--EAAN---------TPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIA 153
Cdd:cd18107   66 EDWvEARKARGTPLQLVGtdEHGDhqiadhdftRPTLLLIGNETAGLSAAWRELCDETVSIPMAGSASSLNAACAASIFL 145


                 ...
gi 446862764 154 YEV 156
Cdd:cd18107  146 YEA 148
TM1570-like cd18085
SPOUT superfamily RNA methyltransferase TM1570-like; DUF2168; This domain, found in various ...
 21-152 4.36e-04

SPOUT superfamily RNA methyltransferase TM1570-like; DUF2168; This domain, found in various hypothetical prokaryotic proteins, has no known function. It is also found in a few prokaryotic tRNA (guanine-N(1)-)-methyltransferases. Proteins of this family are members of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349958  Cd Length: 178  Bit Score: 39.83  E-value: 4.36e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  21 VARAMKTMGLTNLWLVNPLvkPDSQAIA--------LAAGAS------DVIGNAHIVDTLDEALAGCS-------LVVGT 79
Cdd:cd18085   30 IARSSRTYGVKGYYIVTPL--EDQQELAerilghwtEGYGGKynpdrkEALSLVRVVESLEEAIAEIEekegkrpLVVAT 107

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446862764  80 SARSR--TLPWPMLdPRECglksvaeAANTPVALVFGRERvGLTNEELQKCHYHVA-IAANPEYSSLNLAMAVQVI 152
Cdd:cd18085  108 SARPYanAISYAEL-RRML-------LEDRPVLLVFGTGW-GLAPEVLERADYVLEpIRGAGDYNHLSVRSAAAII 174
SpoU-like_RNA-MTase cd18109
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 107-155 3.41e-03

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349982  Cd Length: 141  Bit Score: 36.75  E-value: 3.41e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 446862764 107 TPVALVFGRERVGLTNEELQKCHYHVAIAANPEYS-SLNLAMAVQVIAYE 155
Cdd:cd18109   91 EKGILVMGNEGHGISPEVLKLLTHRLTIPRYGKGAeSLNVAVATAILLSE 140
SpoU-like cd18096
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 7-155 7.77e-03

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349969  Cd Length: 140  Bit Score: 35.65  E-value: 7.77e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764   7 IVLVETSHTGNMGSVARAMKTMGLTNLWLVNplvkpdSQAIAL--AAGASDVIgNAHIVDTLDEALA-----GCSLV--- 76
Cdd:cd18096    3 LVLHNVAKRHNIGTIVRSANAFGVKEVHLVG------RRKFNPfgAMGTDRHV-KFRHFDTLAEAKEylkerGIRLVgve 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446862764  77 VGTSARS-RTLPWPmldpRECglksvaeaantpvALVFGRERVGLTNEELQKCHYHVAIaanPEYS----SLNLAMAVQV 151
Cdd:cd18096   76 IVPGAEPiETHPFP----GDT-------------AFVLGNEGTGLSDEQLAICDGFVYI---PQYGsgtaSLNVSVAASI 135


                 ....
gi 446862764 152 IAYE 155
Cdd:cd18096  136 VLHH 139
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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