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Conserved domains on  [gi|446948302|ref|WP_001025558|]
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proline racemase family protein [Bacillus thuringiensis]

Protein Classification

4-hydroxyproline epimerase; proline racemase family protein( domain architecture ID 1905033)

4-hydroxyproline epimerase catalyzes the epimerization of trans-4-hydroxy-L-proline (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp)| proline racemase family protein similar to trans-3-hydroxy-L-proline (t3LHyp) dehydratase (aka 4-hydroxyproline 2-epimerase) that catalyzes the dehydration of t3LHyp to delta(1)-pyrroline-2-carboxylate (Pyr2C), and the epimerization of trans-4-hydroxy-L-proline (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Pro_racemase super family cl42407
Proline racemase; This family consists of proline racemase (EC 5.1.1.4) proteins which ...
 1-313 1.66e-38

Proline racemase; This family consists of proline racemase (EC 5.1.1.4) proteins which catalyze the interconversion of L- and D-proline in bacteria. This family also contains several similar eukaryotic proteins including Swiss:Q9NCP4 a sequence with B-cell mitogenic properties which has been characterized as a co-factor-independent proline racemase.


The actual alignment was detected with superfamily member COG3938:

Pssm-ID: 478179  Cd Length: 335  Bit Score: 138.69  E-value: 1.66e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302   1 MNIQKMYTAVDVHVNGEAFRVM---------KDVpckyyysLEQLNEqfsgeLAEEM----KLLLNEPRGFIGLNGCIVV 67
Cdd:COG3938    1 MRSSRTITTIDAHTEGEPTRVVtggvpplpgNTM-------LEKRRY-----FAENYdwlrRLLMFEPRGHADMSGALLV 68

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302  68 PSIHNEVDAAVLFFNHEGSIPL--HygGIVAVITMLLESGYLKKRES-NHYKIETLSGIFLVHAYVENDEVISVSFESKL 144
Cdd:COG3938   69 PPTRPDADFGVIFMETSGYLPMcgH--GTIGVATVLLETGLVPMTEPvTEVVLDTPAGLVRARAEVEDGKVESVTFENVP 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 145 CYMIEENLQV-----GNVSYSLIQADKVYAVVEKGANSPEIRVENISELKRWGEVTLQTIQKQ-----------SLIKRL 208
Cdd:COG3938  147 SFVYALDVEVevpglGTVTVDIAYGGNFYAIVDAQQLGLDLEPENARELIRLGLEIRDAANEQlevvhpenpdiNGVSHV 226

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 209 ILVDPSQTEKNHIKSITFHEDNFIVRSP-GfvsTIVSF-VHTLF-KNDYIADKPFINESIFNS-FI-TVKKVKKEE--TG 281
Cdd:COG3938  227 EFTGPPTDPDADARNAVVFGPGAIDRSPcG---TGTSArMALLHaRGELKVGETFVHESIIGSrFTgRILEETTVGgyPA 303

                        330       340       350
                 ....*....|....*....|....*....|..
gi 446948302 282 YIFRFESRGFITGMQTFLLDPTDPFPTGFLLK 313
Cdd:COG3938  304 IIPEITGRAWITGFNQFVLDPDDPLPEGFLLR 335
 
Name Accession Description Interval E-value
PrdF COG3938
Proline racemase/hydroxyproline epimerase [Amino acid transport and metabolism];
1-313 1.66e-38

Proline racemase/hydroxyproline epimerase [Amino acid transport and metabolism];


Pssm-ID: 443139  Cd Length: 335  Bit Score: 138.69  E-value: 1.66e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302   1 MNIQKMYTAVDVHVNGEAFRVM---------KDVpckyyysLEQLNEqfsgeLAEEM----KLLLNEPRGFIGLNGCIVV 67
Cdd:COG3938    1 MRSSRTITTIDAHTEGEPTRVVtggvpplpgNTM-------LEKRRY-----FAENYdwlrRLLMFEPRGHADMSGALLV 68

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302  68 PSIHNEVDAAVLFFNHEGSIPL--HygGIVAVITMLLESGYLKKRES-NHYKIETLSGIFLVHAYVENDEVISVSFESKL 144
Cdd:COG3938   69 PPTRPDADFGVIFMETSGYLPMcgH--GTIGVATVLLETGLVPMTEPvTEVVLDTPAGLVRARAEVEDGKVESVTFENVP 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 145 CYMIEENLQV-----GNVSYSLIQADKVYAVVEKGANSPEIRVENISELKRWGEVTLQTIQKQ-----------SLIKRL 208
Cdd:COG3938  147 SFVYALDVEVevpglGTVTVDIAYGGNFYAIVDAQQLGLDLEPENARELIRLGLEIRDAANEQlevvhpenpdiNGVSHV 226

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 209 ILVDPSQTEKNHIKSITFHEDNFIVRSP-GfvsTIVSF-VHTLF-KNDYIADKPFINESIFNS-FI-TVKKVKKEE--TG 281
Cdd:COG3938  227 EFTGPPTDPDADARNAVVFGPGAIDRSPcG---TGTSArMALLHaRGELKVGETFVHESIIGSrFTgRILEETTVGgyPA 303

                        330       340       350
                 ....*....|....*....|....*....|..
gi 446948302 282 YIFRFESRGFITGMQTFLLDPTDPFPTGFLLK 313
Cdd:COG3938  304 IIPEITGRAWITGFNQFVLDPDDPLPEGFLLR 335
Pro_racemase pfam05544
Proline racemase; This family consists of proline racemase (EC 5.1.1.4) proteins which ...
 10-311 1.37e-28

Proline racemase; This family consists of proline racemase (EC 5.1.1.4) proteins which catalyze the interconversion of L- and D-proline in bacteria. This family also contains several similar eukaryotic proteins including Swiss:Q9NCP4 a sequence with B-cell mitogenic properties which has been characterized as a co-factor-independent proline racemase.


Pssm-ID: 398923  Cd Length: 325  Bit Score: 112.01  E-value: 1.37e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302   10 VDVHVNGEAFRVM----KDVPCkyyyslEQLNEQfSGELAEEM----KLLLNEPRGFIGLNGCIVVPSIHNEVDAAVLFF 81
Cdd:pfam05544   2 VDSHTEGEPGRVVvggvPPLPG------ATMAEK-RRYFARDHdhlrRFLMFEPRGHDMMSGALLVPPKRPDADAGVIFM 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302   82 NHEGSIPLHYGGIVAVITMLLESGYLKKRE-SNHYKIETLSGIFLVHAYVENDEVISVSFESKLCYMIEENLQV-----G 155
Cdd:pfam05544  75 EPSGYLPMCGHNTIGVVTVLLETGIVPPQEpETRLNLETPAGLVEARAECRDGKVERVSLRNVPSFVYALDVEVevpglG 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302  156 NVSYSLIQADKVYAVVEKGANSPEIRVENISELKRWGEVTLQTIQKQ-----------SLIKRLILVDPSQTEKNHIKSI 224
Cdd:pfam05544 155 RVRVDIAYGGNFYAIVDAEQLGFDIEPAEARELVAAGEKIRDALNEQlkvvhpenpdiRGVSHVQLTGPPTRAEAHARNA 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302  225 TFHEDNFIVRSPGFVSTIVSFVHTLFKNDYIADKPFINESIFNSFITVKKVKKEETG----YIFRFESRGFITGMQTFLL 300
Cdd:pfam05544 235 VIIGPGAIDRSPCGTGTSARMAQLHAKGKLKVGETFVHESIIGSEFEGRVEGETEVGgrpaIVPSISGRAWITGTNQLLL 314

                         330
                  ....*....|.
gi 446948302  301 DPTDPFPTGFL 311
Cdd:pfam05544 315 DPSDPFAHGFR 325
PRK13969 PRK13969
proline racemase; Provisional
 1-312 6.66e-19

proline racemase; Provisional


Pssm-ID: 184427 [Multi-domain]  Cd Length: 334  Bit Score: 85.68  E-value: 6.66e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302   1 MNIQKMYTAVDVHVNGEAFRV----MKDVPCKyyySLEQLNEQFSGELAEEMKLLLNEPRGFIGLNGCIVVPSIHNEVDA 76
Cdd:PRK13969   1 MRFSKSIQTIDSHTMGEPTRIvvggLPKIPGK---TMAEKKEYLEENNDSLRTALMSEPRGHNDMFGSIYTEPADETADF 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302  77 AVLFFNHEGSIPLHYGGIVAVITMLLESGYLKKRES-NHYKIETLSGIFLVHAYVENDEVISVSFESKLCYMIEENL--- 152
Cdd:PRK13969  78 GIIFMDGGGYLNMCGHGSIGAATCAVETGIVKVEEPyTHIKLEAPAGMINARVKVEDGKAKEVSIVNVPAFLYKKDVeid 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 153 --QVGNVSYSLIQADKVYAVVEKGANSPEIRVENISELKRWG----EVTLQTIQKQ----SLIKRLILV---DPSQTEKN 219
Cdd:PRK13969 158 vpDYGKVTLDISFGGSFFAIVDAEQLGIKISPANSQKLNDLGmkirHAVNEQIEIKhpvlEHIKTVDLVeiyDEAKHPDA 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 220 HIKSITFHEDNFIVRSPGFVSTIVSFVHTLFKNDYIADKPFINESIFNSFITVKKVKKEETG----YIFRFESRGFITGM 295
Cdd:PRK13969 238 DYKNVVIFGQGQVDRSPCGTGTSAKMATLYAKGKLKVGEEFVYESIIGTKFKGKILEETKVGeydaIIPEITGSAYITGF 317

                        330
                 ....*....|....*..
gi 446948302 296 QTFLLDPTDPFPTGFLL 312
Cdd:PRK13969 318 NQFVIDEEDPVKYGFVL 334
 
Name Accession Description Interval E-value
PrdF COG3938
Proline racemase/hydroxyproline epimerase [Amino acid transport and metabolism];
1-313 1.66e-38

Proline racemase/hydroxyproline epimerase [Amino acid transport and metabolism];


Pssm-ID: 443139  Cd Length: 335  Bit Score: 138.69  E-value: 1.66e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302   1 MNIQKMYTAVDVHVNGEAFRVM---------KDVpckyyysLEQLNEqfsgeLAEEM----KLLLNEPRGFIGLNGCIVV 67
Cdd:COG3938    1 MRSSRTITTIDAHTEGEPTRVVtggvpplpgNTM-------LEKRRY-----FAENYdwlrRLLMFEPRGHADMSGALLV 68

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302  68 PSIHNEVDAAVLFFNHEGSIPL--HygGIVAVITMLLESGYLKKRES-NHYKIETLSGIFLVHAYVENDEVISVSFESKL 144
Cdd:COG3938   69 PPTRPDADFGVIFMETSGYLPMcgH--GTIGVATVLLETGLVPMTEPvTEVVLDTPAGLVRARAEVEDGKVESVTFENVP 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 145 CYMIEENLQV-----GNVSYSLIQADKVYAVVEKGANSPEIRVENISELKRWGEVTLQTIQKQ-----------SLIKRL 208
Cdd:COG3938  147 SFVYALDVEVevpglGTVTVDIAYGGNFYAIVDAQQLGLDLEPENARELIRLGLEIRDAANEQlevvhpenpdiNGVSHV 226

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 209 ILVDPSQTEKNHIKSITFHEDNFIVRSP-GfvsTIVSF-VHTLF-KNDYIADKPFINESIFNS-FI-TVKKVKKEE--TG 281
Cdd:COG3938  227 EFTGPPTDPDADARNAVVFGPGAIDRSPcG---TGTSArMALLHaRGELKVGETFVHESIIGSrFTgRILEETTVGgyPA 303

                        330       340       350
                 ....*....|....*....|....*....|..
gi 446948302 282 YIFRFESRGFITGMQTFLLDPTDPFPTGFLLK 313
Cdd:COG3938  304 IIPEITGRAWITGFNQFVLDPDDPLPEGFLLR 335
Pro_racemase pfam05544
Proline racemase; This family consists of proline racemase (EC 5.1.1.4) proteins which ...
 10-311 1.37e-28

Proline racemase; This family consists of proline racemase (EC 5.1.1.4) proteins which catalyze the interconversion of L- and D-proline in bacteria. This family also contains several similar eukaryotic proteins including Swiss:Q9NCP4 a sequence with B-cell mitogenic properties which has been characterized as a co-factor-independent proline racemase.


Pssm-ID: 398923  Cd Length: 325  Bit Score: 112.01  E-value: 1.37e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302   10 VDVHVNGEAFRVM----KDVPCkyyyslEQLNEQfSGELAEEM----KLLLNEPRGFIGLNGCIVVPSIHNEVDAAVLFF 81
Cdd:pfam05544   2 VDSHTEGEPGRVVvggvPPLPG------ATMAEK-RRYFARDHdhlrRFLMFEPRGHDMMSGALLVPPKRPDADAGVIFM 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302   82 NHEGSIPLHYGGIVAVITMLLESGYLKKRE-SNHYKIETLSGIFLVHAYVENDEVISVSFESKLCYMIEENLQV-----G 155
Cdd:pfam05544  75 EPSGYLPMCGHNTIGVVTVLLETGIVPPQEpETRLNLETPAGLVEARAECRDGKVERVSLRNVPSFVYALDVEVevpglG 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302  156 NVSYSLIQADKVYAVVEKGANSPEIRVENISELKRWGEVTLQTIQKQ-----------SLIKRLILVDPSQTEKNHIKSI 224
Cdd:pfam05544 155 RVRVDIAYGGNFYAIVDAEQLGFDIEPAEARELVAAGEKIRDALNEQlkvvhpenpdiRGVSHVQLTGPPTRAEAHARNA 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302  225 TFHEDNFIVRSPGFVSTIVSFVHTLFKNDYIADKPFINESIFNSFITVKKVKKEETG----YIFRFESRGFITGMQTFLL 300
Cdd:pfam05544 235 VIIGPGAIDRSPCGTGTSARMAQLHAKGKLKVGETFVHESIIGSEFEGRVEGETEVGgrpaIVPSISGRAWITGTNQLLL 314

                         330
                  ....*....|.
gi 446948302  301 DPTDPFPTGFL 311
Cdd:pfam05544 315 DPSDPFAHGFR 325
PRK13969 PRK13969
proline racemase; Provisional
 1-312 6.66e-19

proline racemase; Provisional


Pssm-ID: 184427 [Multi-domain]  Cd Length: 334  Bit Score: 85.68  E-value: 6.66e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302   1 MNIQKMYTAVDVHVNGEAFRV----MKDVPCKyyySLEQLNEQFSGELAEEMKLLLNEPRGFIGLNGCIVVPSIHNEVDA 76
Cdd:PRK13969   1 MRFSKSIQTIDSHTMGEPTRIvvggLPKIPGK---TMAEKKEYLEENNDSLRTALMSEPRGHNDMFGSIYTEPADETADF 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302  77 AVLFFNHEGSIPLHYGGIVAVITMLLESGYLKKRES-NHYKIETLSGIFLVHAYVENDEVISVSFESKLCYMIEENL--- 152
Cdd:PRK13969  78 GIIFMDGGGYLNMCGHGSIGAATCAVETGIVKVEEPyTHIKLEAPAGMINARVKVEDGKAKEVSIVNVPAFLYKKDVeid 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 153 --QVGNVSYSLIQADKVYAVVEKGANSPEIRVENISELKRWG----EVTLQTIQKQ----SLIKRLILV---DPSQTEKN 219
Cdd:PRK13969 158 vpDYGKVTLDISFGGSFFAIVDAEQLGIKISPANSQKLNDLGmkirHAVNEQIEIKhpvlEHIKTVDLVeiyDEAKHPDA 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 220 HIKSITFHEDNFIVRSPGFVSTIVSFVHTLFKNDYIADKPFINESIFNSFITVKKVKKEETG----YIFRFESRGFITGM 295
Cdd:PRK13969 238 DYKNVVIFGQGQVDRSPCGTGTSAKMATLYAKGKLKVGEEFVYESIIGTKFKGKILEETKVGeydaIIPEITGSAYITGF 317

                        330
                 ....*....|....*..
gi 446948302 296 QTFLLDPTDPFPTGFLL 312
Cdd:PRK13969 318 NQFVIDEEDPVKYGFVL 334
PRK13971 PRK13971
4-hydroxyproline epimerase;
49-313 5.61e-15

4-hydroxyproline epimerase;


Pssm-ID: 184428  Cd Length: 333  Bit Score: 74.22  E-value: 5.61e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302  49 KLLLNEPRGFIGLNGCIVVPSIHNEVDAAVLFFNHEGSIPLHYGGIVAVITMLLESGYLKKRESNHYKIETLSGIFLVHA 128
Cdd:PRK13971  49 TGLMFEPRGHDMMSGSILYPPTRPDCDFAILFIETSGCLPMCGHGTIGTVTAAIEEGLVTPKTPGKLRLDTPAGLVDIEY 128

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 129 YVENDEVISVSFESKLCYMIEENLQV-----GNVSYSLIQADKVYAVVEKGANSPEIRVENISELKRWGEVTLQTIQKQ- 202
Cdd:PRK13971 129 EQDGEKVESVRLTNVPSYLAAEDLEVeipglGPLKVDVAYGGNFYAIVEPQENFPGLDHYSASDILRWSPVLRQALNEKy 208

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446948302 203 -------SLIKRL---ILVDPSQTEKNHIKSITFHEDNFIVRSPGFVSTIVSFVHTLFKNDYIADKPFINESIFNSfITV 272
Cdd:PRK13971 209 efvhpedPRIRGVshvLWTGKPISPGADARNAVFYGDKAIDRSPCGTGTSARMAQLAAKGKLKVGDEFVHESIIGS-LFE 287

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*.
gi 446948302 273 KKVKKEET-----GYIFRFESRGFITGMQTFLLDPTDPFPTGFLLK 313
Cdd:PRK13971 288 GRVEAAATvggrpAILPSIEGWARVTGHNTIFVDDRDPYAHGFQVK 333
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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