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Conserved domains on  [gi|446989429|ref|WP_001066685|]
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MULTISPECIES: hypothetical protein [Gammaproteobacteria]

Protein Classification

nucleotide exchange factor GrpE( domain architecture ID 1562)

nucleotide exchange factor GrpE functions as a nucleotide exchange factor for DnaK and participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins; may also function as a thermosensor

CATH:  3.90.20.20|2.30.22.10
Gene Ontology:  GO:0000774|GO:0051087|GO:0006457|GO:0042803
SCOP:  4003783|4002724

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GrpE super family cl03075
nucleotide exchange factor GrpE; GrpE is the adenine nucleotide exchange factor of DnaK (Hsp70) ...
 2-95 5.55e-04

nucleotide exchange factor GrpE; GrpE is the adenine nucleotide exchange factor of DnaK (Hsp70)-type ATPases. In bacteria, the DnaK-DnaJ-GrpE (KJE) chaperone system functions at the fulcrum of protein homeostasis. GrpE participates actively in response to heat shock by preventing aggregation of stress-denatured proteins; unfolded proteins initially bind to DnaJ, the J-domain ATPase-activating protein (Hsp40 family), whereupon DnaK hydrolyzes its bound ATP, resulting in a stable complex. The GrpE dimer binds to the ATPase domain of Hsp70 catalyzing the dissociation of ADP, which enables rebinding of ATP, one step in the Hsp70 reaction cycle in protein folding. In eukaryotes, only the mitochondrial Hsp70, not the cytosolic form, is GrpE dependent. Over-expression of Hsp70 molecular chaperones is important in suppressing toxicity of aberrantly folded proteins that occur in Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis, as well as several polyQ-diseases such as Huntington's disease and ataxias.


The actual alignment was detected with superfamily member PRK14143:

Pssm-ID: 446000 [Multi-domain]  Cd Length: 238  Bit Score: 37.40  E-value: 5.55e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446989429   2 NNVAQTNDESVKAENDAANTNSNETKAKDESGNIEDyLSPKGKKLAQRKEQMKRELELLDEQ---IAQEVE---QAKLKV 75
Cdd:PRK14143  29 EEVTEQEAELTNPEGDAAEAESSPDSGSAASETAAD-NAARLAQLEQELESLKQELEELNSQymrIAADFDnfrKRTSRE 107

                         90       100
                 ....*....|....*....|
gi 446989429  76 KDDLFKLLDRNKLLEISPKV 95
Cdd:PRK14143 108 QEDLRLQLKCNTLSEILPVV 127
 
Name Accession Description Interval E-value
PRK14143 PRK14143
heat shock protein GrpE; Provisional
 2-95 5.55e-04

heat shock protein GrpE; Provisional


Pssm-ID: 237624 [Multi-domain]  Cd Length: 238  Bit Score: 37.40  E-value: 5.55e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446989429   2 NNVAQTNDESVKAENDAANTNSNETKAKDESGNIEDyLSPKGKKLAQRKEQMKRELELLDEQ---IAQEVE---QAKLKV 75
Cdd:PRK14143  29 EEVTEQEAELTNPEGDAAEAESSPDSGSAASETAAD-NAARLAQLEQELESLKQELEELNSQymrIAADFDnfrKRTSRE 107

                         90       100
                 ....*....|....*....|
gi 446989429  76 KDDLFKLLDRNKLLEISPKV 95
Cdd:PRK14143 108 QEDLRLQLKCNTLSEILPVV 127
 
Name Accession Description Interval E-value
PRK14143 PRK14143
heat shock protein GrpE; Provisional
 2-95 5.55e-04

heat shock protein GrpE; Provisional


Pssm-ID: 237624 [Multi-domain]  Cd Length: 238  Bit Score: 37.40  E-value: 5.55e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446989429   2 NNVAQTNDESVKAENDAANTNSNETKAKDESGNIEDyLSPKGKKLAQRKEQMKRELELLDEQ---IAQEVE---QAKLKV 75
Cdd:PRK14143  29 EEVTEQEAELTNPEGDAAEAESSPDSGSAASETAAD-NAARLAQLEQELESLKQELEELNSQymrIAADFDnfrKRTSRE 107

                         90       100
                 ....*....|....*....|
gi 446989429  76 KDDLFKLLDRNKLLEISPKV 95
Cdd:PRK14143 108 QEDLRLQLKCNTLSEILPVV 127
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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