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Conserved domains on  [gi|447050246|ref|WP_001127502|]
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MULTISPECIES: type VI secretion system PAAR protein [Enterobacteriaceae]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
T6SS_PAAR_dom NF033420
type VI secretion system PAAR domain; The PAAR domain is widespread, but this model represents ...
 1-81 3.20e-47

type VI secretion system PAAR domain; The PAAR domain is widespread, but this model represents a narrow clade that may occur in type VI secretion systems (T6SS), either free-standing or fused to a long extension. Effector domains of T6SS may be separate proteins, or may be fused to on of the tube or spike proteins: VgrG (spike), Hcp (tube), or this PAAR family (spike tip). Members of this family that have long extensions are likely to be T6SS effectors.


:

Pssm-ID: 411112  Cd Length: 94  Bit Score: 145.50  E-value: 3.20e-47
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447050246  1 MPLAARLNDKGTQHDGYRETVIIAASSTVFIDGLPAARQGDPLTPHDKPKHPPHPRKIARGSSTVFIDGLPAARTGDAID 80
Cdd:NF033420  1 MGNAALLGDIGTDHDGFPPTPVIAGSPTVKIDGKPAARQGDPLAPHDKPKHPPHPRSIAGGSSTVFIDGKPAARTGDAVS 80


                .
gi 447050246 81 C 81
Cdd:NF033420 81 C 81
 
Name Accession Description Interval E-value
T6SS_PAAR_dom NF033420
type VI secretion system PAAR domain; The PAAR domain is widespread, but this model represents ...
 1-81 3.20e-47

type VI secretion system PAAR domain; The PAAR domain is widespread, but this model represents a narrow clade that may occur in type VI secretion systems (T6SS), either free-standing or fused to a long extension. Effector domains of T6SS may be separate proteins, or may be fused to on of the tube or spike proteins: VgrG (spike), Hcp (tube), or this PAAR family (spike tip). Members of this family that have long extensions are likely to be T6SS effectors.


Pssm-ID: 411112  Cd Length: 94  Bit Score: 145.50  E-value: 3.20e-47
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447050246  1 MPLAARLNDKGTQHDGYRETVIIAASSTVFIDGLPAARQGDPLTPHDKPKHPPHPRKIARGSSTVFIDGLPAARTGDAID 80
Cdd:NF033420  1 MGNAALLGDIGTDHDGFPPTPVIAGSPTVKIDGKPAARQGDPLAPHDKPKHPPHPRSIAGGSSTVFIDGKPAARTGDAVS 80


                .
gi 447050246 81 C 81
Cdd:NF033420 81 C 81
PAAR_1 cd14737
proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR ...
 2-81 6.15e-37

proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family, where it forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). The T6SS is responsible for translocation of a wide variety of toxic effector molecules, allowing predatory cells to kill prokaryotic as well as eukaryotic prey cells. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes. It has been shown that PAAR proteins are essential for T6SS-mediated secretion and target cell killing by Vibrio cholerae (encodes two PAAR proteins) and Acinetobacter baylyi (encodes three PAAR proteins); inactivation of all these PAAR genes results in inactivation of Hcp secretion as well as T6SS-dependent killing of E. coli.


Pssm-ID: 269822  Cd Length: 94  Bit Score: 119.69  E-value: 6.15e-37
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447050246  2 PLAARLNDKGTQHDGYRETVIIAASSTVFIDGLPAARQGDPLTPHDKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDC 81
Cdd:cd14737   1 PAAARLGDICTGHGGFPPTPVIAGSPDVTVNGKPVLRQGDALAPHTCPKHPPHGGVIASGSSTVFINGKPAARVGDPVSC 80
PAAR COG4104
Zn-binding Pro-Ala-Ala-Arg (PAAR) domain, involved in Type VI secretion [Intracellular ...
 1-81 2.23e-21

Zn-binding Pro-Ala-Ala-Arg (PAAR) domain, involved in Type VI secretion [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443280  Cd Length: 87  Bit Score: 79.86  E-value: 2.23e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447050246  1 MPLAARLNDKgTQHDGyretVIIAASSTVFIDGLPAARQGDPLTPhdkPKHPPHPrkIARGSSTVFIDGLPAARTGDAID 80
Cdd:COG4104   2 PKPAARLGDK-TSHGG----PVISGSPTVLIGGRPAARVGDKVSC---PKHGPDT--IAEGSPTVLINGKPAARVGDKTA 71


                .
gi 447050246 81 C 81
Cdd:COG4104  72 C 72
PAAR_motif pfam05488
PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. ...
 21-81 8.09e-17

PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. It is also found as a triplet of tandem repeats comprising the entire length in a another family of hypothetical proteins.


Pssm-ID: 428491  Cd Length: 71  Bit Score: 67.98  E-value: 8.09e-17
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 447050246  21 VIIAASSTVFIDGLPAARQGDPLTPhdkPKHPpHPRKIARGSSTVFIDGLPAARTGDAIDC 81
Cdd:pfam05488 10 VVITGSPTVLIGGKPAARVGDLVVC---PPCG-GGGPIAEGSPTVLINGKPAAREGDKTAC 66
 
Name Accession Description Interval E-value
T6SS_PAAR_dom NF033420
type VI secretion system PAAR domain; The PAAR domain is widespread, but this model represents ...
 1-81 3.20e-47

type VI secretion system PAAR domain; The PAAR domain is widespread, but this model represents a narrow clade that may occur in type VI secretion systems (T6SS), either free-standing or fused to a long extension. Effector domains of T6SS may be separate proteins, or may be fused to on of the tube or spike proteins: VgrG (spike), Hcp (tube), or this PAAR family (spike tip). Members of this family that have long extensions are likely to be T6SS effectors.


Pssm-ID: 411112  Cd Length: 94  Bit Score: 145.50  E-value: 3.20e-47
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447050246  1 MPLAARLNDKGTQHDGYRETVIIAASSTVFIDGLPAARQGDPLTPHDKPKHPPHPRKIARGSSTVFIDGLPAARTGDAID 80
Cdd:NF033420  1 MGNAALLGDIGTDHDGFPPTPVIAGSPTVKIDGKPAARQGDPLAPHDKPKHPPHPRSIAGGSSTVFIDGKPAARTGDAVS 80


                .
gi 447050246 81 C 81
Cdd:NF033420 81 C 81
PAAR_1 cd14737
proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR ...
 2-81 6.15e-37

proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family, where it forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). The T6SS is responsible for translocation of a wide variety of toxic effector molecules, allowing predatory cells to kill prokaryotic as well as eukaryotic prey cells. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes. It has been shown that PAAR proteins are essential for T6SS-mediated secretion and target cell killing by Vibrio cholerae (encodes two PAAR proteins) and Acinetobacter baylyi (encodes three PAAR proteins); inactivation of all these PAAR genes results in inactivation of Hcp secretion as well as T6SS-dependent killing of E. coli.


Pssm-ID: 269822  Cd Length: 94  Bit Score: 119.69  E-value: 6.15e-37
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447050246  2 PLAARLNDKGTQHDGYRETVIIAASSTVFIDGLPAARQGDPLTPHDKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDC 81
Cdd:cd14737   1 PAAARLGDICTGHGGFPPTPVIAGSPDVTVNGKPVLRQGDALAPHTCPKHPPHGGVIASGSSTVFINGKPAARVGDPVSC 80
PAAR COG4104
Zn-binding Pro-Ala-Ala-Arg (PAAR) domain, involved in Type VI secretion [Intracellular ...
 1-81 2.23e-21

Zn-binding Pro-Ala-Ala-Arg (PAAR) domain, involved in Type VI secretion [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443280  Cd Length: 87  Bit Score: 79.86  E-value: 2.23e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447050246  1 MPLAARLNDKgTQHDGyretVIIAASSTVFIDGLPAARQGDPLTPhdkPKHPPHPrkIARGSSTVFIDGLPAARTGDAID 80
Cdd:COG4104   2 PKPAARLGDK-TSHGG----PVISGSPTVLIGGRPAARVGDKVSC---PKHGPDT--IAEGSPTVLINGKPAARVGDKTA 71


                .
gi 447050246 81 C 81
Cdd:COG4104  72 C 72
PAAR_like cd14671
proline-alanine-alanine-arginine (PAAR) repeat superfamily; This domain is found in the PAAR ...
 4-81 1.63e-20

proline-alanine-alanine-arginine (PAAR) repeat superfamily; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat superfamily, where it forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). The T6SS is responsible for translocation of a wide variety of toxic effector molecules, allowing predatory cells to kill prokaryotic as well as eukaryotic prey cells. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. The PAAR-repeat proteins form a diverse superfamily with several subgroups extended both N- and C-terminally by domains with various predicted functions; the termini are exposed to solution, and do not distort the VgrG binding site. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes. It has been shown that PAAR proteins are essential for T6SS-mediated secretion and target cell killing by Vibrio cholerae (encodes two PAAR proteins) and Acinetobacter baylyi (encodes three PAAR proteins); inactivation of all these PAAR genes results in inactivation of Hcp secretion as well as T6SS-dependent killing of E. coli.


Pssm-ID: 269821  Cd Length: 77  Bit Score: 77.36  E-value: 1.63e-20
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 447050246  4 AARLNDkGTQHDGyrETVIIAASSTVFIDGLPAARQGDPLTphdkpkHPPHPRKIARGSSTVFIDGLPAARTGDAIDC 81
Cdd:cd14671   2 AARVGD-PTAHTP--GGPVISGSPNVFINGRPAARVGDVGD------HPGGGNAIVSGSGTVFINGKPAARVGDRTSC 70
PAAR_RHS cd14742
proline-alanine-alanine-arginine (PAAR) domain, also containing C-terminal Rearrangement ...
 4-81 1.78e-17

proline-alanine-alanine-arginine (PAAR) domain, also containing C-terminal Rearrangement hotspot (Rhs) extensions; This PAAR (proline-alanine-alanine-arginine) repeat subfamily, which forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS), contains C- and N-terminal domain extensions. These include Rearrangement hotspot (Rhs) protein repeats and conserved Rhs repeat-associated unique core sequences at the C-terminal, and various predicted functions at N- and C-terminal extensions. However, these terminal domains are exposed to solution, and do not distort the binding site of VgrG. Rhs and related YD-peptide repeat proteins are widely distributed in bacteria. Rhs shares similar architecture with distantly related WapA proteins of Bacillus and Listeria species, suggesting intercellular growth inhibition as its primary function. Additionally, a plasmid-encoded Rhs protein has been implicated in bacteriocin production in Pseudomonas savastanoi. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes.


Pssm-ID: 269827  Cd Length: 86  Bit Score: 69.93  E-value: 1.78e-17
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 447050246  4 AARLNDkGTQHDGyretVIIAASSTVFIDGLPAARQGDPLTPHDKpkHPPHPRKIARGSSTVFIDGLPAARTGDAIDC 81
Cdd:cd14742   2 AARVGD-PIAHTG----TITSGSPNVFINGKPAARAADSTVACSK--HPPPPQLIAEGSETVFINGQPAARKGDKTTC 72
PAAR_motif pfam05488
PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. ...
 21-81 8.09e-17

PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. It is also found as a triplet of tandem repeats comprising the entire length in a another family of hypothetical proteins.


Pssm-ID: 428491  Cd Length: 71  Bit Score: 67.98  E-value: 8.09e-17
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 447050246  21 VIIAASSTVFIDGLPAARQGDPLTPhdkPKHPpHPRKIARGSSTVFIDGLPAARTGDAIDC 81
Cdd:pfam05488 10 VVITGSPTVLIGGKPAARVGDLVVC---PPCG-GGGPIAEGSPTVLINGKPAAREGDKTAC 66
PAAR_2 cd14738
proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR ...
 21-78 1.54e-13

proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family, where it forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). The T6SS is responsible for translocation of a wide variety of toxic effector molecules, allowing predatory cells to kill prokaryotic as well as eukaryotic prey cells. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes. It has been shown that PAAR proteins are essential for T6SS-mediated secretion and target cell killing by Vibrio cholerae (encodes two PAAR proteins) and Acinetobacter baylyi (encodes three PAAR proteins); inactivation of all these PAAR genes results in inactivation of Hcp secretion as well as T6SS-dependent killing of E. coli.


Pssm-ID: 269823  Cd Length: 94  Bit Score: 60.34  E-value: 1.54e-13
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 447050246 21 VIIAASSTVFIDGLPAARQGDPLTPhdkpkhPPHPRKIARGSSTVFIDGLPAARTGDA 78
Cdd:cd14738  26 IVGPGPTTVLIGGLPAARVGDMCVC------VGPPDTIVQGSSTVLIGGKPAARMGDS 77
PAAR_5 cd14741
proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR ...
 2-81 1.59e-10

proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family in bacteria as well as some archaea, where it forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). The T6SS is responsible for translocation of a wide variety of toxic effector molecules, allowing predatory cells to kill prokaryotic as well as eukaryotic prey cells. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes. It has been shown that PAAR proteins are essential for T6SS-mediated secretion and target cell killing by Vibrio cholerae (encodes two PAAR proteins) and Acinetobacter baylyi (encodes three PAAR proteins); inactivation of all these PAAR genes results in inactivation of Hcp secretion as well as T6SS-dependent killing of E. coli.


Pssm-ID: 269826  Cd Length: 95  Bit Score: 52.78  E-value: 1.59e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447050246  2 PLAARLNDKgTQHDGYRETViiAASSTVFIDGLPAAR-QGDPLTPHDKPKHPPH-PRKIARGSSTVFIDGLPAARTGDAI 79
Cdd:cd14741   1 PPAARIGDS-TAHGGPLTPG--PGSPNVLIGGFPAWRaGGDGHVCPLVTGPVPHvGGVVAAGSTTVLINGLPAARMGDMI 77


                ..
gi 447050246 80 DC 81
Cdd:cd14741  78 VE 79
PAAR_CT_2 cd14744
proline-alanine-alanine-arginine (PAAR) domain with uncharacterized C-terminal extension; This ...
 5-81 3.68e-09

proline-alanine-alanine-arginine (PAAR) domain with uncharacterized C-terminal extension; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family of mostly beta- and gamma-proteobacteria, and forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). Most members contain C-terminal domain extensions corresponding to several uncharacterized domains such as S-type pyocin, DUF2235, DUF2345 and cytotoxic proteins. However, these terminal domains are exposed to solution, and do not distort the binding site of VgrG. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes.


Pssm-ID: 269829  Cd Length: 78  Bit Score: 48.70  E-value: 3.68e-09
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 447050246  5 ARLNDKgTQHDGyretVIIAASSTVFIDGLPAARQGDPLT-PHDKPKHPphprkIARGSSTVFIDGLPAARTGDAIDC 81
Cdd:cd14744   4 IRLGDK-TTHGG----VVISGSSTFTIDGRPVARVGDKVTcPKCKGTGP-----IVEGGPTFTVDGRPVALDGDRVAC 71
PAAR_4 cd14740
proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR ...
 26-77 3.84e-09

proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family of bacteria, and forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). A few members contains C-terminal domain extensions corresponding to Rearrangement hotspot (Rhs) protein repeats and conserved Rhs repeat-associated unique core sequences as well as uncharacterized domains such as DUF4150. However, these terminal domains are exposed to solution, and do not distort the binding site of VgrG. Rhs and related YD-peptide repeat proteins are widely distributed in bacteria. Rhs shares similar architecture with distantly related WapA proteins of Bacillus and Listeria species, suggesting intercellular growth inhibition as its primary function. Additionally, a plasmid-encoded Rhs protein has been implicated in bacteriocin production in Pseudomonas savastanoi. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes.


Pssm-ID: 269825  Cd Length: 121  Bit Score: 49.73  E-value: 3.84e-09
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 447050246  26 SSTVFIDGLPAARQGD--PLTPHDKPKHPPHPRK----IARGSSTVFIDGLPAARTGD 77
Cdd:cd14740   41 SPTVLIGGMPAATVGStaGNTPGGVPGGPSVPPAnpgtIVMGSSTVFINGKPAARMGD 98
PAAR_3 cd14739
proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR ...
 4-81 4.32e-09

proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family, where it forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). The T6SS is responsible for translocation of a wide variety of toxic effector molecules, allowing predatory cells to kill prokaryotic as well as eukaryotic prey cells. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes. It has been shown that PAAR proteins are essential for T6SS-mediated secretion and target cell killing by Vibrio cholerae (encodes two PAAR proteins) and Acinetobacter baylyi (encodes three PAAR proteins); inactivation of all these PAAR genes results in inactivation of Hcp secretion as well as T6SS-dependent killing of E. coli.


Pssm-ID: 269824  Cd Length: 90  Bit Score: 48.89  E-value: 4.32e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447050246  4 AARLNDKgTQHDGyreTVIIAASSTVFIDGLPAARQGD----PLTPHdkPKHPPHPRKIArGSSTVFIDGLPAARTGDAI 79
Cdd:cd14739   2 AARVGDP-TTHGG---TITGPGVPTVLIGGMPAAVVGDmhacVIPPP--PAHPPASPFPP-GSATVLIGGRPAARVGDAC 74


                ..
gi 447050246 80 DC 81
Cdd:cd14739  75 GC 76
PAAR_CT_1 cd14743
proline-alanine-alanine-arginine (PAAR) domain with C-terminal extension; This domain is found ...
 20-81 7.82e-08

proline-alanine-alanine-arginine (PAAR) domain with C-terminal extension; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family of mostly gamma-proteobacteria, and forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). Some members contains C-terminal domain extensions corresponding to Rearrangement hotspot (Rhs) protein repeats and conserved Rhs repeat-associated unique core sequences as well as uncharacterized domains. However, these terminal domains are exposed to solution, and do not distort the binding site of VgrG. Rhs and related YD-peptide repeat proteins are widely distributed in bacteria. Rhs shares similar architecture with distantly related WapA proteins of Bacillus and Listeria species, suggesting intercellular growth inhibition as its primary function. Additionally, a plasmid-encoded Rhs protein has been implicated in bacteriocin production in Pseudomonas savastanoi. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes.


Pssm-ID: 269828  Cd Length: 78  Bit Score: 45.37  E-value: 7.82e-08
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 447050246 20 TVIIAASSTVFIDGLPAARQGDPLTPHDKpkhpphprkIARGSSTVFIDGLPAARTGDAIDC 81
Cdd:cd14743  18 STPIGSSSADFFDGLPAARVGDKTSCGAT---------IVSGSINVLINGKPAAVLGSTTSH 70
PAAR_CT_1 cd14743
proline-alanine-alanine-arginine (PAAR) domain with C-terminal extension; This domain is found ...
 35-81 5.71e-06

proline-alanine-alanine-arginine (PAAR) domain with C-terminal extension; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family of mostly gamma-proteobacteria, and forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). Some members contains C-terminal domain extensions corresponding to Rearrangement hotspot (Rhs) protein repeats and conserved Rhs repeat-associated unique core sequences as well as uncharacterized domains. However, these terminal domains are exposed to solution, and do not distort the binding site of VgrG. Rhs and related YD-peptide repeat proteins are widely distributed in bacteria. Rhs shares similar architecture with distantly related WapA proteins of Bacillus and Listeria species, suggesting intercellular growth inhibition as its primary function. Additionally, a plasmid-encoded Rhs protein has been implicated in bacteriocin production in Pseudomonas savastanoi. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes.


Pssm-ID: 269828  Cd Length: 78  Bit Score: 40.36  E-value: 5.71e-06
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 447050246 35 PAARQGDPltpHDKPKHPpHPRKIARGSSTVFIDGLPAARTGDAIDC 81
Cdd:cd14743   1 PAARVGDP---HACPLPG-HGSTPIGSSSADFFDGLPAARVGDKTSC 43
PAAR_motif pfam05488
PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. ...
 4-44 1.20e-05

PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. It is also found as a triplet of tandem repeats comprising the entire length in a another family of hypothetical proteins.


Pssm-ID: 428491  Cd Length: 71  Bit Score: 39.48  E-value: 1.20e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 447050246   4 AARLNDKGTQHDGYRETVIIAASSTVFIDGLPAARQGDPLT 44
Cdd:pfam05488 25 AARVGDLVVCPPCGGGGPIAEGSPTVLINGKPAAREGDKTA 65
PAAR_4 cd14740
proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR ...
 21-64 5.36e-03

proline-alanine-alanine-arginine (PAAR) domain; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family of bacteria, and forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). A few members contains C-terminal domain extensions corresponding to Rearrangement hotspot (Rhs) protein repeats and conserved Rhs repeat-associated unique core sequences as well as uncharacterized domains such as DUF4150. However, these terminal domains are exposed to solution, and do not distort the binding site of VgrG. Rhs and related YD-peptide repeat proteins are widely distributed in bacteria. Rhs shares similar architecture with distantly related WapA proteins of Bacillus and Listeria species, suggesting intercellular growth inhibition as its primary function. Additionally, a plasmid-encoded Rhs protein has been implicated in bacteriocin production in Pseudomonas savastanoi. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes.


Pssm-ID: 269825  Cd Length: 121  Bit Score: 33.56  E-value: 5.36e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 447050246  21 VIIAASSTVFIDGLPAARQGDPLTPHDKPKHPPHPRKIARGSST 64
Cdd:cd14740   78 TIVMGSSTVFINGKPAARMGDMTATCNDPAPAPVGTVVPPGATV 121
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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