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Conserved domains on  [gi|447159431|ref|WP_001236687|]
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PTS mannitol transporter subunit IIA [Escherichia coli]

Protein Classification

PTS sugar transporter subunit IIA( domain architecture ID 76)

PTS sugar transporter subunit IIA is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system that catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

CATH:  3.40.930.10
Gene Ontology:  GO:1902495|GO:0009401|GO:0016310|GO:0016301|GO:0008643
PubMed:  11532441|10393270
SCOP:  4001876

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PTS_IIA_fru super family cl00163
PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: ...
 1-147 1.96e-90

PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.


The actual alignment was detected with superfamily member PRK09854:

Pssm-ID: 469635 [Multi-domain]  Cd Length: 147  Bit Score: 259.15  E-value: 1.96e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431   1 MRLIDYFPELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQAIKDSTISNGPYYILAPGVAMPHARPECGALKTGMS 80
Cdd:PRK09854   1 MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGALKTGMS 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 447159431  81 LTLLKQGVYFPGNDEPIKLLIGLSAADADSHIAAIQALSELLCEEEILEQLLTASSEKQLAGIISRG 147
Cdd:PRK09854  81 LTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG 147
 
Name Accession Description Interval E-value
cmtB PRK09854
PTS mannitol transporter subunit IIA;
1-147 1.96e-90

PTS mannitol transporter subunit IIA;


Pssm-ID: 182114 [Multi-domain]  Cd Length: 147  Bit Score: 259.15  E-value: 1.96e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431   1 MRLIDYFPELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQAIKDSTISNGPYYILAPGVAMPHARPECGALKTGMS 80
Cdd:PRK09854   1 MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGALKTGMS 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 447159431  81 LTLLKQGVYFPGNDEPIKLLIGLSAADADSHIAAIQALSELLCEEEILEQLLTASSEKQLAGIISRG 147
Cdd:PRK09854  81 LTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG 147
PTS_IIA_fru cd00211
PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: ...
 7-116 8.07e-26

PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.


Pssm-ID: 238129 [Multi-domain]  Cd Length: 136  Bit Score: 94.94  E-value: 8.07e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431   7 FPELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQAIKDSTiSNGPYYILaPGVAMPHARPECgALKTGMSLTLLKQ 86
Cdd:cd00211    1 LTKENIRLNLKAKSKEEAIEELAQLLVAAGYVEEEYIEALLERE-KEGSTGIG-NGIAIPHAKSEA-VKKPGIAVLRLKE 77

                         90       100       110
                 ....*....|....*....|....*....|.
gi 447159431  87 GVYFPGND-EPIKLLIGLSAADADSHIAAIQ 116
Cdd:cd00211   78 PVDFGSLDgQPVHLIFLLAAPDSNEHLKALS 108
PtsN COG1762
Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate ...
 1-117 1.64e-21

Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate transport and metabolism, Signal transduction mechanisms];


Pssm-ID: 441368 [Multi-domain]  Cd Length: 150  Bit Score: 84.13  E-value: 1.64e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431   1 MRLIDYFPELSISVIHSTKDWQEAINFSMASLLAKNYIS--ENYIQAIKDSTISNGPYyiLAPGVAMPHARPEcGALKTG 78
Cdd:COG1762    1 MMLSDLLTPELILLDLEASSKEEAIEELAELLAEKGYVLdkEEYLEALLEREELGSTG--IGPGIAIPHARPE-GVKKPG 77

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 447159431  79 MSLTLLKQGVYFPGND-EPIKLLIGLSA--ADADSHIAAIQA 117
Cdd:COG1762   78 IAVARLKEPVDFGAMDgEPVDLVFLLAApeDDSEEHLKLLAE 119
PTS_EIIA_2 pfam00359
Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;
6-116 3.18e-20

Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;


Pssm-ID: 459780 [Multi-domain]  Cd Length: 139  Bit Score: 80.71  E-value: 3.18e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431    6 YFPELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQAIKDSTISNGPYYILapGVAMPHARPEcGALKTGMSLTLLK 85
Cdd:pfam00359   1 LLDKELIFLNLEAKSKEEAIEFLADKLVEAGYVEPAYLEAILEREKEGSTGIGN--GIAIPHARSE-AVKKPGIAVLTLK 77

                          90       100       110
                  ....*....|....*....|....*....|...
gi 447159431   86 QGVYFPGND-EPIKLLIGLSAADADS-HIAAIQ 116
Cdd:pfam00359  78 EPVDFGSEDgKPVKLIFLLAAPDNEAsHLKILS 110
fruA TIGR00848
PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family ...
 6-116 3.02e-03

PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family Bacterial PTS transporters transport and concomitantly phosphorylate their sugar substrates, and typically consist of multiple subunits or protein domains. The Fru family is a large and complex family which includes several sequenced fructose and mannitol-specific permeases as well as several putative PTS permeases of unknown specificities. The fructose permeases of this family phosphorylate fructose on the 1-position. Those of family 4.6 phosphorylate fructose on the 6-position. The Fru family PTS systems typically have 3 domains, IIA, IIB and IIC, which may be found as 1 or more proteins. The fructose and mannitol transporters form separate phylogenetic clusters in this family. This model is specific for the IIA domain of the fructose PTS transporters. Also similar to the Enzyme IIA Fru subunits of the PTS, but included in TIGR01419 rather than this model, is enzyme IIA Ntr (nitrogen), also called PtsN, found in E. coli and other organisms, which may play a solely regulatory role. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 273298 [Multi-domain]  Cd Length: 129  Bit Score: 35.71  E-value: 3.02e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431    6 YFPELSISVIHSTKDWQEAINFSMASLLAKNYIS--ENYIQAIKD--STISNGpyyiLAPGVAMPHARPECgALKTGMSL 81
Cdd:TIGR00848   1 LLNKDLIFLDQQFTSKEDVIKFLANKLLENGYISdtEEFLEDLLKreEEGTTG----IGDGVAIPHAKSAA-VKQPFVAI 75

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 447159431   82 TLLKQGVYFPGND-EPIKL--LIGLSAADA-DSHIAAIQ 116
Cdd:TIGR00848  76 ARLVKGVDWQSLDgKPVKLifLIAVPKDEAgNTHLKALS 114
 
Name Accession Description Interval E-value
cmtB PRK09854
PTS mannitol transporter subunit IIA;
1-147 1.96e-90

PTS mannitol transporter subunit IIA;


Pssm-ID: 182114 [Multi-domain]  Cd Length: 147  Bit Score: 259.15  E-value: 1.96e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431   1 MRLIDYFPELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQAIKDSTISNGPYYILAPGVAMPHARPECGALKTGMS 80
Cdd:PRK09854   1 MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGALKTGMS 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 447159431  81 LTLLKQGVYFPGNDEPIKLLIGLSAADADSHIAAIQALSELLCEEEILEQLLTASSEKQLAGIISRG 147
Cdd:PRK09854  81 LTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG 147
PRK10372 PRK10372
PTS ascorbate transporter subunit IIA;
1-111 2.64e-27

PTS ascorbate transporter subunit IIA;


Pssm-ID: 182417 [Multi-domain]  Cd Length: 154  Bit Score: 99.28  E-value: 2.64e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431   1 MRLIDYFPE-LSISVIHSTKDWQEAINFSMASLLAKNYISENYIQAIKDSTISNGPYYILAPGVAMPHARPECGALKTGM 79
Cdd:PRK10372   1 MKLRDSLAEnKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGF 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 447159431  80 SLTLLKQGVYFPGND-EPIKLLIGLSAADADSH 111
Cdd:PRK10372  81 ALVTLKKPLEFNHEDnDPVDILITMAAVDANTH 113
PTS_IIA_fru cd00211
PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: ...
 7-116 8.07e-26

PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.


Pssm-ID: 238129 [Multi-domain]  Cd Length: 136  Bit Score: 94.94  E-value: 8.07e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431   7 FPELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQAIKDSTiSNGPYYILaPGVAMPHARPECgALKTGMSLTLLKQ 86
Cdd:cd00211    1 LTKENIRLNLKAKSKEEAIEELAQLLVAAGYVEEEYIEALLERE-KEGSTGIG-NGIAIPHAKSEA-VKKPGIAVLRLKE 77

                         90       100       110
                 ....*....|....*....|....*....|.
gi 447159431  87 GVYFPGND-EPIKLLIGLSAADADSHIAAIQ 116
Cdd:cd00211   78 PVDFGSLDgQPVHLIFLLAAPDSNEHLKALS 108
PtsN COG1762
Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate ...
 1-117 1.64e-21

Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate transport and metabolism, Signal transduction mechanisms];


Pssm-ID: 441368 [Multi-domain]  Cd Length: 150  Bit Score: 84.13  E-value: 1.64e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431   1 MRLIDYFPELSISVIHSTKDWQEAINFSMASLLAKNYIS--ENYIQAIKDSTISNGPYyiLAPGVAMPHARPEcGALKTG 78
Cdd:COG1762    1 MMLSDLLTPELILLDLEASSKEEAIEELAELLAEKGYVLdkEEYLEALLEREELGSTG--IGPGIAIPHARPE-GVKKPG 77

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 447159431  79 MSLTLLKQGVYFPGND-EPIKLLIGLSA--ADADSHIAAIQA 117
Cdd:COG1762   78 IAVARLKEPVDFGAMDgEPVDLVFLLAApeDDSEEHLKLLAE 119
PTS_EIIA_2 pfam00359
Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;
6-116 3.18e-20

Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;


Pssm-ID: 459780 [Multi-domain]  Cd Length: 139  Bit Score: 80.71  E-value: 3.18e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431    6 YFPELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQAIKDSTISNGPYYILapGVAMPHARPEcGALKTGMSLTLLK 85
Cdd:pfam00359   1 LLDKELIFLNLEAKSKEEAIEFLADKLVEAGYVEPAYLEAILEREKEGSTGIGN--GIAIPHARSE-AVKKPGIAVLTLK 77

                          90       100       110
                  ....*....|....*....|....*....|...
gi 447159431   86 QGVYFPGND-EPIKLLIGLSAADADS-HIAAIQ 116
Cdd:pfam00359  78 EPVDFGSEDgKPVKLIFLLAAPDNEAsHLKILS 110
MtlA2 COG4668
Mannitol/fructose-specific phosphotransferase system, IIA domain [Carbohydrate transport and ...
 11-147 1.12e-11

Mannitol/fructose-specific phosphotransferase system, IIA domain [Carbohydrate transport and metabolism];


Pssm-ID: 443705 [Multi-domain]  Cd Length: 143  Bit Score: 58.63  E-value: 1.12e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431  11 SISVIHSTKDWQEAINFSMASLLAKNYISENYIQAIKDSTISNGPYyiLAPGVAMPHARPECGA--LKTGMSLTLLKQGV 88
Cdd:COG4668    8 NIRLNASAANKEEAIRLAGQLLVEAGYVEPEYIDAMLEREAQVSTY--LGNGIAIPHGTNEAKDlvLKTGISVLQFPDGV 85

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 447159431  89 YFpGNDEPIKLLIGLSAADaDSHIAAIQALSELLCEEEILEQLLTASSEKQLAGIISRG 147
Cdd:COG4668   86 DW-GDGNTVYLVIGIAAKS-DEHLEILRQLARVLSDEENVEKLAKATDAEEILALLTGE 142
PRK15083 PRK15083
PTS system mannitol-specific transporter subunit IICBA; Provisional
 22-117 4.88e-05

PTS system mannitol-specific transporter subunit IICBA; Provisional


Pssm-ID: 237905 [Multi-domain]  Cd Length: 639  Bit Score: 41.96  E-value: 4.88e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431  22 QEAINFSMASLLAKNYISENYIQAIKDSTISNGPYyiLAPGVAMPHARPECG--ALKTGMSLTLLKQGVYF-PGNDEPIK 98
Cdd:PRK15083 512 EEAIRFAGEQLVKGGYVEPEYVDAMLDREKLTSTY--LGESIAVPHGTVEAKdrVLKTGVVFCQYPEGVRFgEEEDDIAR 589

                         90
                 ....*....|....*....
gi 447159431  99 LLIGLsAADADSHIAAIQA 117
Cdd:PRK15083 590 LVIGI-AARNNEHIQVITS 607
PRK13779 PRK13779
bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein; Provisional
 8-113 1.26e-03

bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein; Provisional


Pssm-ID: 237502 [Multi-domain]  Cd Length: 503  Bit Score: 37.93  E-value: 1.26e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431   8 PELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQAIKDSTISNGPYyiLAPGVAMPHARPECGAL--KTGMSLTLLK 85
Cdd:PRK13779   5 SESNIHLNAQAINKQQAIEMAAAALEQAGNVENGYLQGMLARELQTSTF--LGNGIAIPHGTLDTRHMvkNTGVQIFQFP 82

                         90       100
                 ....*....|....*....|....*...
gi 447159431  86 QGVYFpGNDEPIKLLIGLsAADADSHIA 113
Cdd:PRK13779  83 QGIEW-GEGNIAYVVIGI-AARSDEHLS 108
fruA TIGR00848
PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family ...
 6-116 3.02e-03

PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family Bacterial PTS transporters transport and concomitantly phosphorylate their sugar substrates, and typically consist of multiple subunits or protein domains. The Fru family is a large and complex family which includes several sequenced fructose and mannitol-specific permeases as well as several putative PTS permeases of unknown specificities. The fructose permeases of this family phosphorylate fructose on the 1-position. Those of family 4.6 phosphorylate fructose on the 6-position. The Fru family PTS systems typically have 3 domains, IIA, IIB and IIC, which may be found as 1 or more proteins. The fructose and mannitol transporters form separate phylogenetic clusters in this family. This model is specific for the IIA domain of the fructose PTS transporters. Also similar to the Enzyme IIA Fru subunits of the PTS, but included in TIGR01419 rather than this model, is enzyme IIA Ntr (nitrogen), also called PtsN, found in E. coli and other organisms, which may play a solely regulatory role. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 273298 [Multi-domain]  Cd Length: 129  Bit Score: 35.71  E-value: 3.02e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431    6 YFPELSISVIHSTKDWQEAINFSMASLLAKNYIS--ENYIQAIKD--STISNGpyyiLAPGVAMPHARPECgALKTGMSL 81
Cdd:TIGR00848   1 LLNKDLIFLDQQFTSKEDVIKFLANKLLENGYISdtEEFLEDLLKreEEGTTG----IGDGVAIPHAKSAA-VKQPFVAI 75

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 447159431   82 TLLKQGVYFPGND-EPIKL--LIGLSAADA-DSHIAAIQ 116
Cdd:TIGR00848  76 ARLVKGVDWQSLDgKPVKLifLIAVPKDEAgNTHLKALS 114
PRK11109 PRK11109
fused PTS fructose transporter subunit IIA/HPr protein;
9-113 3.20e-03

fused PTS fructose transporter subunit IIA/HPr protein;


Pssm-ID: 236849 [Multi-domain]  Cd Length: 375  Bit Score: 36.46  E-value: 3.20e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447159431   9 ELSISVIH---STKDWQEAINFSMASLLAKNYISENYIQAIKDSTISNGPYyiLAPGVAMPHARPECGAL--KTGMSLTL 83
Cdd:PRK11109   3 QLSVQDIHlgqQAGNKEEAIRQVAAALTQAGNVAEGYVDGMLAREQQTSTF--LGNGIAIPHGTTDTRDLvlKTGVQVFQ 80

                         90       100       110
                 ....*....|....*....|....*....|
gi 447159431  84 LKQGVYFpGNDEPIKLLIGLsAADADSHIA 113
Cdd:PRK11109  81 FPQGVTW-GDGQTAYVAIGI-AAKSDEHLG 108
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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