NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|447199198|ref|WP_001276454|]
View 

MULTISPECIES: prepilin-type N-terminal cleavage/methylation domain-containing protein [Escherichia]

Protein Classification

prepilin-type N-terminal cleavage/methylation domain-containing protein( domain architecture ID 11484661)

prepilin-type N-terminal cleavage/methylation domain-containing protein similar to Escherichia coli prepilin peptidase-dependent protein C

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
PRK10332 PRK10332
prepilin-type N-terminal cleavage/methylation domain-containing protein;
1-106 4.14e-62

prepilin-type N-terminal cleavage/methylation domain-containing protein;


:

Pssm-ID: 182384 [Multi-domain]  Cd Length: 107  Bit Score: 184.22  E-value: 4.14e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447199198   1 MSASLKHQHGFSLPEVILAMVLMVVIVTALSGYQRTLSTSIASRNQYQQLWRYAWQQTQLRAISPPANWQVNRMQTSQAG 80
Cdd:PRK10332   1 MSASLKNQRGFSLPEVLLAMVLMVMIVTALSGYQRTLMNSFASRNQYRQLWRHAWQQTQLRAISPPANWQVNRMQTSQAG 80

                         90       100
                 ....*....|....*....|....*.
gi 447199198  81 CVSISVTLFSPGGREGEMTRLHCPNR 106
Cdd:PRK10332  81 CVSISVTLVSPGGRQGQMTRLHCPNR 106
 
Name Accession Description Interval E-value
PRK10332 PRK10332
prepilin-type N-terminal cleavage/methylation domain-containing protein;
1-106 4.14e-62

prepilin-type N-terminal cleavage/methylation domain-containing protein;


Pssm-ID: 182384 [Multi-domain]  Cd Length: 107  Bit Score: 184.22  E-value: 4.14e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447199198   1 MSASLKHQHGFSLPEVILAMVLMVVIVTALSGYQRTLSTSIASRNQYQQLWRYAWQQTQLRAISPPANWQVNRMQTSQAG 80
Cdd:PRK10332   1 MSASLKNQRGFSLPEVLLAMVLMVMIVTALSGYQRTLMNSFASRNQYRQLWRHAWQQTQLRAISPPANWQVNRMQTSQAG 80

                         90       100
                 ....*....|....*....|....*.
gi 447199198  81 CVSISVTLFSPGGREGEMTRLHCPNR 106
Cdd:PRK10332  81 CVSISVTLVSPGGRQGQMTRLHCPNR 106
T2SSppdC pfam12528
Type II secretion prepilin peptidase dependent protein C;
33-104 4.35e-22

Type II secretion prepilin peptidase dependent protein C;


Pssm-ID: 432611  Cd Length: 80  Bit Score: 82.13  E-value: 4.35e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447199198   33 YQRTLSTSIASRNQYQQLWRYAWQQTQLR-------AISPPANWQVNRM-QTSQAGCVSISVTLFSPGGREGEMTRLHCP 104
Cdd:pfam12528   1 YQQALLQGFQRQWQQRQAWRLAHQQLELYsaptalgALSPPPGWQVERMeQTVSAGCVSVTVTLTSPQGRQVELSRWHCP 80
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
1-87 4.95e-13

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 59.23  E-value: 4.95e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447199198   1 MSASLKHQHGFSLPEVILAMVLMVVIVTALSGYQRTLSTSIASRNQYQQLWRYAWQQT-QLRAISPPANwqvnrmqtSQA 79
Cdd:COG4967    3 RRRRRRRQRGFTLIEVLVALVILSIGLLGLAGLQAASLRSSQDARQRTQAALLAQDLLeRLRANPAAAG--------SYP 74


                 ....*...
gi 447199198  80 GCVSISVT 87
Cdd:COG4967   75 GIKSITVT 82
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 8-31 5.31e-03

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 31.89  E-value: 5.31e-03
                          10        20
                  ....*....|....*....|....
gi 447199198    8 QHGFSLPEVILAMVLMVVIVTALS 31
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIAL 24
 
Name Accession Description Interval E-value
PRK10332 PRK10332
prepilin-type N-terminal cleavage/methylation domain-containing protein;
1-106 4.14e-62

prepilin-type N-terminal cleavage/methylation domain-containing protein;


Pssm-ID: 182384 [Multi-domain]  Cd Length: 107  Bit Score: 184.22  E-value: 4.14e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447199198   1 MSASLKHQHGFSLPEVILAMVLMVVIVTALSGYQRTLSTSIASRNQYQQLWRYAWQQTQLRAISPPANWQVNRMQTSQAG 80
Cdd:PRK10332   1 MSASLKNQRGFSLPEVLLAMVLMVMIVTALSGYQRTLMNSFASRNQYRQLWRHAWQQTQLRAISPPANWQVNRMQTSQAG 80

                         90       100
                 ....*....|....*....|....*.
gi 447199198  81 CVSISVTLFSPGGREGEMTRLHCPNR 106
Cdd:PRK10332  81 CVSISVTLVSPGGRQGQMTRLHCPNR 106
T2SSppdC pfam12528
Type II secretion prepilin peptidase dependent protein C;
33-104 4.35e-22

Type II secretion prepilin peptidase dependent protein C;


Pssm-ID: 432611  Cd Length: 80  Bit Score: 82.13  E-value: 4.35e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447199198   33 YQRTLSTSIASRNQYQQLWRYAWQQTQLR-------AISPPANWQVNRM-QTSQAGCVSISVTLFSPGGREGEMTRLHCP 104
Cdd:pfam12528   1 YQQALLQGFQRQWQQRQAWRLAHQQLELYsaptalgALSPPPGWQVERMeQTVSAGCVSVTVTLTSPQGRQVELSRWHCP 80
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
1-87 4.95e-13

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 59.23  E-value: 4.95e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 447199198   1 MSASLKHQHGFSLPEVILAMVLMVVIVTALSGYQRTLSTSIASRNQYQQLWRYAWQQT-QLRAISPPANwqvnrmqtSQA 79
Cdd:COG4967    3 RRRRRRRQRGFTLIEVLVALVILSIGLLGLAGLQAASLRSSQDARQRTQAALLAQDLLeRLRANPAAAG--------SYP 74


                 ....*...
gi 447199198  80 GCVSISVT 87
Cdd:COG4967   75 GIKSITVT 82
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 1-50 1.90e-06

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 43.08  E-value: 1.90e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 447199198   1 MSASLKHQHGFSLPEVILAMVLM-VVIVTALSGYQRTLSTSIASRNQYQQL 50
Cdd:COG4795    1 MKRARRRQRGFTLLELLVALAIFaLLLLAAYRGLDSVLRSRERLEQQAERL 51
PulK COG3156
Type II secretory pathway, component PulK [Intracellular trafficking, secretion, and vesicular ...
 1-54 5.99e-05

Type II secretory pathway, component PulK [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 442390 [Multi-domain]  Cd Length: 307  Bit Score: 39.98  E-value: 5.99e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 447199198   1 MSASLKHQHGFSLPEVILAMVLMVVIVTALSGYQRTLSTSIASRNQYQQLWRYA 54
Cdd:COG3156    1 MSRRRRRQRGVALITVLLIVALLAALAAALAERQRLELRRAENLLDRQQARWYA 54
PilW COG4966
Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];
5-62 6.07e-05

Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];


Pssm-ID: 443992 [Multi-domain]  Cd Length: 158  Bit Score: 39.78  E-value: 6.07e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 447199198   5 LKHQHGFSLPEVILAMVLMVVIVTALsgyqrtLSTSIASRNQYQQLWRYAWQQTQLRA 62
Cdd:COG4966    1 RRRQRGFTLVELMVALAIGLIVLAAV------LQLFLSSRRSYRTQEALARLQENGRF 52
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 6-31 6.58e-04

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 34.27  E-value: 6.58e-04
                          10        20
                  ....*....|....*....|....*.
gi 447199198    6 KHQHGFSLPEVILAMVLMVVIVTALS 31
Cdd:pfam07963   2 RKQRGFTLIELLVALAILAILLAAAL 27
PRK10557 PRK10557
prepilin peptidase-dependent protein;
6-56 1.16e-03

prepilin peptidase-dependent protein;


Pssm-ID: 236714  Cd Length: 192  Bit Score: 36.11  E-value: 1.16e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 447199198   6 KHQHGFSLPEVILAMVLMVVIVTALSGYQRTLSTSIASRNQYQQLWRYAWQ 56
Cdd:PRK10557   4 VKQRGFSLLEVLLAMAIGSVLLLGAARFLPALQRQILTLTRQLRLEDEIWQ 54
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
1-64 3.57e-03

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 33.28  E-value: 3.57e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 447199198   1 MSASLKHQHGFSLPEVILAMVLMVVIVT-ALSGYQRTLSTSiaSRNQYQQLWRYAWQQTQLRAIS 64
Cdd:COG4970    1 MKRLRRRQRGFTLIELLVVLAILAILAAiAVPSFSSLIARQ--RLRAAANELAAALRLARSEAIR 63
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 8-31 5.31e-03

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 31.89  E-value: 5.31e-03
                          10        20
                  ....*....|....*....|....
gi 447199198    8 QHGFSLPEVILAMVLMVVIVTALS 31
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIAL 24
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 1-50 9.26e-03

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 32.96  E-value: 9.26e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 447199198   1 MSASLKHQHGFSLPEVILAMV----LMVVIVTALSGYQRTLSTSIAsRNQYQQL 50
Cdd:COG2165    3 LRRRRRRQRGFTLIELLVVIAiigiLAALALPALQGARERARRAEL-RSNLRQI 55
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH