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Conserved domains on  [gi|487747223|ref|WP_001829361|]
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MULTISPECIES: NADPH-dependent oxidoreductase [Staphylococcus]

Protein Classification

NADPH-dependent oxidoreductase( domain architecture ID 10114867)

NADPH-dependent oxidoreductase, similar to nitroreductase NfsA, which catalyzes the reduction of flavin or nitrocompounds using NAD(P)H as electron donor

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 7-236 5.65e-93

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


:

Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 272.96  E-value: 5.65e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   7 ELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEVSG-QPYVLDNGYLFVFVLDYYR 85
Cdd:cd02146    3 ETILNHRSVRKFTDEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELAGnQPYVAQAPVFLVFCADLYR 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  86 HHLVDEvAASNMETSYGSAEGLLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLRNDVARVREILNLPDYTFPLFGMAVGE 165
Cdd:cd02146   83 HQKIAE-EAGGKDVGLDYLESFLVGVVDAALAAQNALVAAESLGLGIVYIGGIRNNPEEVIELLGLPEYVFPLFGLTVGH 161

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 487747223 166 PsDEENGSpKPRLPFKHIFHKDQYDANqhQQRKELEAYDQVVSEYYKERTNGVRTENWSQQI-ETFLGRKTR 236
Cdd:cd02146  162 P-DPTPEV-KPRLPLEAVVHEETYQDL--TDLEDLAAYDETMSEYYAERGSNKRTETWSQQIaDRYLSKRDR 229
 
Name Accession Description Interval E-value
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 7-236 5.65e-93

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 272.96  E-value: 5.65e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   7 ELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEVSG-QPYVLDNGYLFVFVLDYYR 85
Cdd:cd02146    3 ETILNHRSVRKFTDEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELAGnQPYVAQAPVFLVFCADLYR 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  86 HHLVDEvAASNMETSYGSAEGLLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLRNDVARVREILNLPDYTFPLFGMAVGE 165
Cdd:cd02146   83 HQKIAE-EAGGKDVGLDYLESFLVGVVDAALAAQNALVAAESLGLGIVYIGGIRNNPEEVIELLGLPEYVFPLFGLTVGH 161

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 487747223 166 PsDEENGSpKPRLPFKHIFHKDQYDANqhQQRKELEAYDQVVSEYYKERTNGVRTENWSQQI-ETFLGRKTR 236
Cdd:cd02146  162 P-DPTPEV-KPRLPLEAVVHEETYQDL--TDLEDLAAYDETMSEYYAERGSNKRTETWSQQIaDRYLSKRDR 229
PRK10765 PRK10765
oxygen-insensitive NADPH nitroreductase;
7-251 2.89e-73

oxygen-insensitive NADPH nitroreductase;


Pssm-ID: 182710  Cd Length: 240  Bit Score: 223.31  E-value: 2.89e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   7 ELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEVSG-QPYVLDNGYLFVFVLDYYR 85
Cdd:PRK10765   6 ELILSHRSIRHFTDEPISEAQREAIINAARAASSSSFLQCSSIIRITDKALREALVELTGgQKYVAQAAEFWVFCADFNR 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  86 HHLVdevaasNMETSYGSAEGLLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLRNDVARVREILNLPDYTFPLFGMAVGE 165
Cdd:PRK10765  86 HLQI------CPDAQLGLAEQLLIGAVDTAIMAQNALLAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGW 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223 166 PSDeeNGSPKPRLPFKHIFHKDQYdanQHQQRKELEAYDQVVSEYYKERTNGVRTENWSQQIETFLGRKTRLDMLDELKK 245
Cdd:PRK10765 160 PAQ--NPDLKPRLPASLLVHENQY---QPLDKDLLAQYDEQLAEYYLTRSSNNRQDTWSDHIRRTLIKESRPFILDYLHK 234


                 ....*.
gi 487747223 246 AGFIQR 251
Cdd:PRK10765 235 QGWATR 240
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 5-184 4.48e-41

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 138.45  E-value: 4.48e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   5 VYELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEV---SGQPYVLDNGYLFVFVL 81
Cdd:COG0778    1 LLELLLTRRSVRKFTDKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEAlaeANQEWVADAPVLIVVCA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  82 DYYRHHLVDEVAASnmetsygsaegllvgtIDVALVAQNMAVAAEDMGYGIVYLGSLrnDVARVREILNLPDYTFPLFGM 161
Cdd:COG0778   81 DPDRSEKVPERYAL----------------LDAGIAAQNLLLAARALGLGTCWIGGF--DPEKVRELLGLPEGEEPVALL 142

                        170       180
                 ....*....|....*....|...
gi 487747223 162 AVGEPSDEENgsPKPRLPFKHIF 184
Cdd:COG0778  143 ALGYPAEELN--PRPRKPLEEVV 163
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 9-164 7.77e-18

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 78.20  E-value: 7.77e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223    9 MKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKE-VSGQPYVLDNGYLFVFV-----LD 82
Cdd:pfam00881   1 IRQRRSVRKFDPEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEaALELLLVEPAAALLLLLrrdanLK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   83 YYRHHLVDE------VAASNMETSYGSAEGLLVGT-IDVALVAQNMAVAAEDMGYGIVYLGSLrnDVARVREILNLPDYT 155
Cdd:pfam00881  81 LLLQDFLRGapvlivITASLSTYLRKAAERAYREAlLDAGAAAQNLLLAATSLGLGSCPIGGF--DAAAVRELLGLPDDE 158


                  ....*....
gi 487747223  156 FPLFGMAVG 164
Cdd:pfam00881 159 RLVGLIAVG 167
 
Name Accession Description Interval E-value
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 7-236 5.65e-93

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 272.96  E-value: 5.65e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   7 ELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEVSG-QPYVLDNGYLFVFVLDYYR 85
Cdd:cd02146    3 ETILNHRSVRKFTDEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELAGnQPYVAQAPVFLVFCADLYR 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  86 HHLVDEvAASNMETSYGSAEGLLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLRNDVARVREILNLPDYTFPLFGMAVGE 165
Cdd:cd02146   83 HQKIAE-EAGGKDVGLDYLESFLVGVVDAALAAQNALVAAESLGLGIVYIGGIRNNPEEVIELLGLPEYVFPLFGLTVGH 161

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 487747223 166 PsDEENGSpKPRLPFKHIFHKDQYDANqhQQRKELEAYDQVVSEYYKERTNGVRTENWSQQI-ETFLGRKTR 236
Cdd:cd02146  162 P-DPTPEV-KPRLPLEAVVHEETYQDL--TDLEDLAAYDETMSEYYAERGSNKRTETWSQQIaDRYLSKRDR 229
PRK10765 PRK10765
oxygen-insensitive NADPH nitroreductase;
7-251 2.89e-73

oxygen-insensitive NADPH nitroreductase;


Pssm-ID: 182710  Cd Length: 240  Bit Score: 223.31  E-value: 2.89e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   7 ELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEVSG-QPYVLDNGYLFVFVLDYYR 85
Cdd:PRK10765   6 ELILSHRSIRHFTDEPISEAQREAIINAARAASSSSFLQCSSIIRITDKALREALVELTGgQKYVAQAAEFWVFCADFNR 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  86 HHLVdevaasNMETSYGSAEGLLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLRNDVARVREILNLPDYTFPLFGMAVGE 165
Cdd:PRK10765  86 HLQI------CPDAQLGLAEQLLIGAVDTAIMAQNALLAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGW 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223 166 PSDeeNGSPKPRLPFKHIFHKDQYdanQHQQRKELEAYDQVVSEYYKERTNGVRTENWSQQIETFLGRKTRLDMLDELKK 245
Cdd:PRK10765 160 PAQ--NPDLKPRLPASLLVHENQY---QPLDKDLLAQYDEQLAEYYLTRSSNNRQDTWSDHIRRTLIKESRPFILDYLHK 234


                 ....*.
gi 487747223 246 AGFIQR 251
Cdd:PRK10765 235 QGWATR 240
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 5-184 4.48e-41

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 138.45  E-value: 4.48e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   5 VYELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEV---SGQPYVLDNGYLFVFVL 81
Cdd:COG0778    1 LLELLLTRRSVRKFTDKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEAlaeANQEWVADAPVLIVVCA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  82 DYYRHHLVDEVAASnmetsygsaegllvgtIDVALVAQNMAVAAEDMGYGIVYLGSLrnDVARVREILNLPDYTFPLFGM 161
Cdd:COG0778   81 DPDRSEKVPERYAL----------------LDAGIAAQNLLLAARALGLGTCWIGGF--DPEKVRELLGLPEGEEPVALL 142

                        170       180
                 ....*....|....*....|...
gi 487747223 162 AVGEPSDEENgsPKPRLPFKHIF 184
Cdd:COG0778  143 ALGYPAEELN--PRPRKPLEEVV 163
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 9-164 2.52e-24

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 94.29  E-value: 2.52e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   9 MKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARL--KEVSGQPYVLDNGYLFVFVLDyyrh 86
Cdd:cd02062    1 IKTRRSIRKFTDKPVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDREKKEKLakLAAPNQKFIAGAPVVIVVVAD---- 76

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 487747223  87 hlvdevaasnmetsygSAEGLLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLRNDVARVREILNLPDYTFPLFGMAVG 164
Cdd:cd02062   77 ----------------PDKSRPWALEDAGAAAQNLLLAAAALGLGSCWIGGFDFREDKVRELLGIPENLRPVALIAIG 138
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 5-187 3.72e-22

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 89.45  E-value: 3.72e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   5 VYELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKE-VSGQPYVLDNGYLFVFVLDy 83
Cdd:cd02139    1 VYEAIKKRRSIRKYKPTPVEEEKLLRILEAARLAPSAKNRQPWRFIVVKDKELKEKLAEaANGQKFIAEAPVVIVACAD- 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  84 yrhhlvdevaasnmETSYGSAEGLLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLRNDvaRVREILNLPDyTFPLFGM-A 162
Cdd:cd02139   80 --------------PSESGMGCGKPYYLVDVAIAMEHLVLAATEEGLGTCWIGAFDED--KVKEILGIPE-EYRVVALtP 142

                        170       180
                 ....*....|....*....|....*
gi 487747223 163 VGEPsdEENGSPKPRLPFKHIFHKD 187
Cdd:cd02139  143 LGYP--AEEPPPRPRKPLEEIVFYE 165
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 7-183 1.98e-18

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 79.21  E-value: 1.98e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   7 ELMKQHHSVRKF-KNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEVS-GQPYVldngylfvfvldyy 84
Cdd:cd02137    2 EVIKSRRSVRNFdPDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAAyNQPQV-------------- 67

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  85 rhhlvdevaasnmetSYGSAEGLLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLrnDVARVREILNLPDYTFPLFGMAVG 164
Cdd:cd02137   68 ---------------TTASAVILVLGDLNAGLAAMNLMLAAKAKGYDTCPMGGF--DKEKVAELLNLPDRYVPVLLIAIG 130

                        170
                 ....*....|....*....
gi 487747223 165 EPSDEenGSPKPRLPFKHI 183
Cdd:cd02137  131 KAADK--APRSGRLPVDEV 147
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 9-164 7.77e-18

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 78.20  E-value: 7.77e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223    9 MKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKE-VSGQPYVLDNGYLFVFV-----LD 82
Cdd:pfam00881   1 IRQRRSVRKFDPEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEaALELLLVEPAAALLLLLrrdanLK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   83 YYRHHLVDE------VAASNMETSYGSAEGLLVGT-IDVALVAQNMAVAAEDMGYGIVYLGSLrnDVARVREILNLPDYT 155
Cdd:pfam00881  81 LLLQDFLRGapvlivITASLSTYLRKAAERAYREAlLDAGAAAQNLLLAATSLGLGSCPIGGF--DAAAVRELLGLPDDE 158


                  ....*....
gi 487747223  156 FPLFGMAVG 164
Cdd:pfam00881 159 RLVGLIAVG 167
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 13-180 1.39e-17

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 76.86  E-value: 1.39e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  13 HSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSiKARLKEVS-GQPYVLdngylfVFVLDYyrhhlvde 91
Cdd:cd02136    6 RSVRAFKDKPVPKETIEKILEAARRAPSGKNTQPWRVYVVTGKA-RERLKKAFfGAPVAL------FLTMDK-------- 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  92 vaasNMETSYgsaegllvgTIDVALVAQNMAVAAEDMGYGIVYLGSLRNDVARVREILNLPDYTFPLFGMAVGEPSDEE- 170
Cdd:cd02136   71 ----VLGPWS---------WFDLGAFLQNLMLAAHALGLGTCPQGALAGYPDVVRKELGIPDDEELVCGIALGYPDPDAp 137

                        170
                 ....*....|.
gi 487747223 171 -NGSPKPRLPF 180
Cdd:cd02136  138 vNQFRTPREPL 148
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 7-164 2.17e-16

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 73.58  E-value: 2.17e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   7 ELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEVS----GQPYVLdngylfvfvld 82
Cdd:cd20609    4 ELAKKRYSVRKFSDKPVEKEKLDKILEAGRLAPTAVNYQPQRILVVRSEEALEKLAKATprffGAPLVI----------- 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  83 yyrhhlvdeVAASNMETSY-GSAEGLLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLrnDVARVREILNLPDYTFPLFGM 161
Cdd:cd20609   73 ---------VVCYDKDESWkRPYDGKDSGDIDAAIVATHMMLAATELGLGTCWVGNF--DPEKVREAFNLPENLEPVAIL 141


                 ...
gi 487747223 162 AVG 164
Cdd:cd20609  142 PLG 144
nitroreductase cd02150
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 14-191 2.24e-15

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380325 [Multi-domain]  Cd Length: 156  Bit Score: 71.09  E-value: 2.24e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  14 SVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEVsgQPY--VLDNGYLFVFVldyyrhhlvde 91
Cdd:cd02150    6 SIRKYTDKPVEEEDIEKLLRAAMAAPSAGNQQPWHFIVVTDREKLDKIAEA--HPYgkMLKEAPLAIVV----------- 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  92 vaASNMETSYgsAEGLLVgtIDVALVAQNMAVAAEDMGYGIVYLGSLRND--VARVREILNLPDYTFPLFGMAVGEPsDE 169
Cdd:cd02150   73 --CGDPSKEK--APGYWV--QDCSAATENILLAAHALGLGAVWLGVYPFEerVKAIREILNIPENIIPFCVIALGYP-AE 145

                        170       180
                 ....*....|....*....|..
gi 487747223 170 EngsPKPrlpfkhifhKDQYDA 191
Cdd:cd02150  146 E---KEP---------KDRFDE 155
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 6-152 1.09e-13

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 66.20  E-value: 1.09e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   6 YELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEVSGQPyvldNGYLfvfvldyyR 85
Cdd:cd20608    1 FEAIKTRRSVRRFSDKPVEEEKLEKILEAARLAPSWANKQCWRFIVVTDKETLSELAKKESPS----NGWL--------K 68

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 487747223  86 HHLVDEVAASNMETSyGSAEGLLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLrnDVARVREILNLP 152
Cdd:cd20608   69 DAPVIIVVCADPKDS-GWLNGQNYYLVDAAIAMQNLMLAATDLGLGTCWIGAF--DEKKVKEILGIP 132
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 7-177 3.68e-10

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 56.77  E-value: 3.68e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   7 ELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEV--SGQPYVLDNGYLFVFVLDyy 84
Cdd:cd02151    1 ELLKKRRSIRKYTDEPIEEEKLEEILEAALLAPSSRNSRPVEFIVVDDKETLKKLSECkpHGSAFLKGAPAAIVVLAD-- 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  85 rhhlvDEVAASNMEtsygsaegllvgtiDVALVAQNMAVAAEDMGYGI----VYLGSLRNDVA---RVREILNLPDYTFP 157
Cdd:cd02151   79 -----TEKSDTWIE--------------DASIAATYIQLAAESLGLGScwiqIRNRETQDGKTaeeYVRELLGIPENYRV 139

                        170       180
                 ....*....|....*....|
gi 487747223 158 LFGMAVGEPsDEEngsPKPR 177
Cdd:cd02151  140 LCIIALGYP-DEE---KPPH 155
iodotyrosine_dehalogenase cd02144
iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the ...
 5-64 3.85e-07

iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the 3, 5 positions of L-tyosine in thyroid, liver and kidney, using NADPH as electron donor. This enzyme is a homolog of the nitroreductase family. These enzymes are usually homodimers.


Pssm-ID: 380320  Cd Length: 192  Bit Score: 49.07  E-value: 3.85e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   5 VYELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEV 64
Cdd:cd02144    1 FYELMKKRRSVRDFSSEPVPREVIENAIRTAGTAPSGANTQPWTFVVVSDPEIKRKIREA 60
nitroreductase_FeS-like cd02143
nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family ...
 8-166 2.77e-05

nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family utilize FMN as a cofactor. This family may be involved in the reduction of flavin or nitroaromatic compounds via an obligatory two-electron transfer. Nitroreductase is homodimer. Each subunit contains one FMN molecule.


Pssm-ID: 380319 [Multi-domain]  Cd Length: 187  Bit Score: 43.62  E-value: 2.77e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   8 LMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARL--------KEVSGQPYVLDNGYLFVF 79
Cdd:cd02143    1 LLRSRRSIRRYKDKPVPRETLEKLLDIARYAPTGHNSQPVHWLVVDDPEKVRRLaelvidwmRELIKEDPELAGKLFLDG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  80 VLDYYRH----------HLVdeVAasnmetsYGSAEGlLVGTIDVALVAQNMAVAAEDMGYGIVYLGSLR---NDVARVR 146
Cdd:cd02143   81 IVAAWEKgidvilrgapHLV--VA-------HAPKDA-PTPPVDCAIALTYLELAAPSLGLGTCWAGFFTaaaNNYPPLR 150

                        170       180
                 ....*....|....*....|
gi 487747223 147 EILNLPDYTFPLFGMAVGEP 166
Cdd:cd02143  151 EALGLPEGHKVGGAMMLGYP 170
TdsD-like cd02138
nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase ...
 8-185 3.49e-05

nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to Burkholderia pseudomallei TdsD, may be involved in the processing of organosulfur compounds. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380315 [Multi-domain]  Cd Length: 174  Bit Score: 42.92  E-value: 3.49e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   8 LMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYS-IIGVEDPSIKARLKEV---SGQPYVlDNGYLFVFVL-- 81
Cdd:cd02138    1 LIAERWSPRAFSPEPISEEDLLSLFEAARWAPSCFNEQPWRfVVARRDTEAFEKLLDLlaeGNQSWA-KNAPVLIVVLak 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  82 -------DYYRHHLVDevaasnmetsYGSAEGLLvgtidvALVAQNMAVAAEDMGyGIvylgslrnDVARVREILNLPDY 154
Cdd:cd02138   80 tefdhngKPNRYALFD----------TGAAVANL------ALQATALGLVVHQMA-GF--------DPEKAKEALGIPDE 134

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 487747223 155 TFPLFGMAVGEPSDEEN---------GSPKPRLPFKHIFH 185
Cdd:cd02138  135 YEPITMIAIGYPGDPESlpekllereEAPRTRKPLSEIVF 174
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 4-80 9.42e-05

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 41.47  E-value: 9.42e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   4 YVYELMKQHHSVRKFK-NQPLGSETVEKLVEAGQsASTSSY-LQTYSIIGVEDPSIKARLKEVS--GQPYVLDNGYLFVF 79
Cdd:cd02149    1 NILELLNFRYATKKFDpNKKISDEDLETILEALR-LSPSSFgLEPWKFLVVENPELKAKLAPAAwfNQPQIKDASHVVVF 79


                 .
gi 487747223  80 V 80
Cdd:cd02149   80 L 80
McbC_SagB-like_oxidoreductase cd02142
oxidase similar to the microcin B17 processing protein McbC; This family is the oxidase domain ...
 53-164 2.79e-04

oxidase similar to the microcin B17 processing protein McbC; This family is the oxidase domain of NRPS (non-ribosomal peptide synthetase) and other systems that modify polypeptides by cyclizing a thioester to form a ring. These include EpoB, part of the epothilone biosynthesis pathway; TubD, part of the tubulysin biosynthesis pathway, MtsD, part of the myxothiozol biosynthesis pathway; IndC, part of the indigoidine biosynthesis pathway and TfxB, part of the trifitoxin processing pathway. All are FMN-dependent and oxidize the product of the cyclization of thioesters in short polypeptides.


Pssm-ID: 380318  Cd Length: 200  Bit Score: 40.87  E-value: 2.79e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  53 EDPSIKARLKEVSGQPYVLDNGYLFVFVLDYYRhhlvdevaasnMETSYGSAeGLLVGTIDVALVAQNMAVAAEDMGYGI 132
Cdd:cd02142  101 EGDFRLDLAHAAGNQAAFGSAAFSLIIVARFER-----------IAWKYGER-AYRYILLEAGHLAQNLYLAATALGLGL 168

                         90       100       110
                 ....*....|....*....|....*....|...
gi 487747223 133 VYLGSLRNDvaRVREILNLPDYT-FPLFGMAVG 164
Cdd:cd02142  169 CAIGAFDDD--ALRELLGLDEVEeVVLYAFVVG 199
BluB cd02145
5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5, ...
 6-179 1.32e-03

5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5,6-dimethylbenzimidazole (DMB), a component of vitamin B12; is is a subfamily of the nitroreductase family; nitroreductases typically reduce their substrates by using NAD(P)H as electron donor and often use FMN as a cofactor.


Pssm-ID: 380321  Cd Length: 196  Bit Score: 38.88  E-value: 1.32e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223   6 YELMKQHHSVRKFKNQPLGSETVEKLVEAGQSASTSSYLQTYSIIGVEDPSIKARLKEV------------SG---QPY- 69
Cdd:cd02145    1 YRVIRWRRDVRHFRPDPVPEEVLERLLQAAHLAPSVGLMQPWRFVRVRSAATRKAVHELfqranaeaaemyTGeraAQYr 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 487747223  70 ------VLDNGYLFVFVLDYYRHHLVDEVAASNMETSYGSaegllvgtidVALVAQNM--AVAAEDMGYGIVylgSLRND 141
Cdd:cd02145   81 tlklegIEEAPLQLAVFCDRARAGGHGLGRTTMPEMDLYS----------SVCAVQNLwlAARAEGLGVGWV---SILDP 147

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 487747223 142 VaRVREILNLPDYTFPLFGMAVGEPsDEENGSP-------KPRLP 179
Cdd:cd02145  148 D-EVKRLLGIPEHWEPVAYLCIGYP-EFFYDEPeleqagwEQRRP 190
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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