Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 325 KVSVVMPIHNASKYLRQTLETVCEQSLREIEIILVENGSTDNTMDIINEFAVKDPRITGISiGKSNPGH--ARNVGISMA 402
Cdd:COG0463    3 LVSVVIPTYNEEEYLEEALESLLAQTYPDFEIIVVDDGSTDGTAEILRELAAKDPRIRVIR-LERNRGKgaARNAGLAAA 81

                         90       100       110
                 ....*....|....*....|....*....|....*..
gi 488332510 403 RGRYLQFLDADDHFEANLLQdAYYRAYDSATDILLFG 439
Cdd:COG0463   82 RGDYIAFLDADDQLDPEKLE-ELVAALEEGPADLVYG 117

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510    7 SVIVPVYNVETYLEEALMSLKNQTLKEIEFLIINDGSTDNSQKIIEEIAQDDPRFRVF-NVKNGGIGKAFNLGVSEAKGE 85
Cdd:pfam00535   1 SVIIPTYNEEKYLLETLESLLNQTYPNFEIIVVDDGSTDGTVEIAEEYAKKDPRVRVIrLPENRGKAGARNAGLRAATGD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   86 YIAEFESDDYVALHAYERLYNTAKSHHADVVRCNWVEFSS-EEEVERDILWQYPDKYNQLIDLKTTDLIVQVYPWNAIYK 164
Cdd:pfam00535  81 YIAFLDADDEVPPDWLEKLVEALEEDGADVVVGSRYVIFGeTGEYRRASRITLSRLPFFLGLRLLGLNLPFLIGGFALYR 160

                  ....*.
gi 488332510  165 KSMIEK 170
Cdd:pfam00535 161 REALEE 166

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   8 VIVPVYNVETYLEEALMSLKNQTLKEIEFLIINDGSTDNSQKIIEEIA-QDDPRFRVFNVKNGGIGKAFNLGVSEAKGEY 86
Cdd:cd00761    1 VIIPAYNEEPYLERCLESLLAQTYPNFEVIVVDDGSTDGTLEILEEYAkKDPRVIRVINEENQGLAAARNAGLKAARGEY 80

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 488332510  87 IAEFESDDYVALHAYERLYNTAKSHH-ADVVRCNW 120
Cdd:cd00761   81 ILFLDADDLLLPDWLERLVAELLADPeADAVGGPG 115

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 328 VVMPIHNASKYLRQTLETVCEQSLREIEIILVENGSTDNTMDIINEFAVKDPRITGISIGK-SNPGHARNVGISMARGRY 406
Cdd:cd00761    1 VIIPAYNEEPYLERCLESLLAQTYPNFEVIVVDDGSTDGTLEILEEYAKKDPRVIRVINEEnQGLAAARNAGLKAARGEY 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 488332510 407 LQFLDADDHFEANLLQDAYYRAYDSATDILLFG 439
Cdd:cd00761   81 ILFLDADDLLLPDWLERLVAELLADPEADAVGG 113

putative glycosyl transferase; Provisional

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 325 KVSVVMPIHNASKYLRQTLETVCEQSLREIEIILVENGSTDNTMDIINEFAVKDPRIT-------GISIgksnpghARNV 397
Cdd:PRK10073   7 KLSIIIPLYNAGKDFRAFMESLIAQTWTALEIIIVNDGSTDNSVEIAKHYAENYPHVRllhqanaGVSV-------ARNT 79

                         90
                 ....*....|....*..
gi 488332510 398 GISMARGRYLQFLDADD 414
Cdd:PRK10073  80 GLAVATGKYVAFPDADD 96

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510    7 SVIVPVYNVETYLEEALMSLKNQTLKEIEFLIINDGSTDNSQKIIEEIAQDDPRFRVF-NVKNGGIGKAFNLGVSEAKGE 85
Cdd:pfam00535   1 SVIIPTYNEEKYLLETLESLLNQTYPNFEIIVVDDGSTDGTVEIAEEYAKKDPRVRVIrLPENRGKAGARNAGLRAATGD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   86 YIAEFESDDYVALHAYERLYNTAKSHHADVVRCNWVEFSS-EEEVERDILWQYPDKYNQLIDLKTTDLIVQVYPWNAIYK 164
Cdd:pfam00535  81 YIAFLDADDEVPPDWLEKLVEALEEDGADVVVGSRYVIFGeTGEYRRASRITLSRLPFFLGLRLLGLNLPFLIGGFALYR 160

                  ....*.
gi 488332510  165 KSMIEK 170
Cdd:pfam00535 161 REALEE 166

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   8 VIVPVYNVETYLEEALMSLKNQTLKEIEFLIINDGSTDNSQKIIEEIA-QDDPRFRVFNVKNGGIGKAFNLGVSEAKGEY 86
Cdd:cd00761    1 VIIPAYNEEPYLERCLESLLAQTYPNFEVIVVDDGSTDGTLEILEEYAkKDPRVIRVINEENQGLAAARNAGLKAARGEY 80

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 488332510  87 IAEFESDDYVALHAYERLYNTAKSHH-ADVVRCNW 120
Cdd:cd00761   81 ILFLDADDLLLPDWLERLVAELLADPeADAVGGPG 115

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 328 VVMPIHNASKYLRQTLETVCEQSLREIEIILVENGSTDNTMDIINEFAVKDPRITGISIGK-SNPGHARNVGISMARGRY 406
Cdd:cd00761    1 VIIPAYNEEPYLERCLESLLAQTYPNFEVIVVDDGSTDGTLEILEEYAKKDPRVIRVINEEnQGLAAARNAGLKAARGEY 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 488332510 407 LQFLDADDHFEANLLQDAYYRAYDSATDILLFG 439
Cdd:cd00761   81 ILFLDADDLLLPDWLERLVAELLADPEADAVGG 113

Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   5 KVSVIVPVYNVETYLEEALMSLKNQT--LKEIEFLIINDGSTDNSQKIIEEIAQDDPRFRVFNV-KNGGIGKAFNLGVSE 81
Cdd:COG1215   30 RVSVIIPAYNEEAVIEETLRSLLAQDypKEKLEVIVVDDGSTDETAEIARELAAEYPRVRVIERpENGGKAAALNAGLKA 109

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 488332510  82 AKGEYIAEFESDDYVALHAYERLYNTAKSHHADVVRCN 119
Cdd:COG1215  110 ARGDIVVFLDADTVLDPDWLRRLVAAFADPGVGASGAN 147

Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   5 KVSVIVPVYNVETYLEEALMSLKNQTLKEIEFLIINDGSTDNSQKIIEEIaqDDPRFRVF-NVKNGGIGKAFNLGVSEAK 83
Cdd:COG1216    4 KVSVVIPTYNRPELLRRCLESLLAQTYPPFEVIVVDNGSTDGTAELLAAL--AFPRVRVIrNPENLGFAAARNLGLRAAG 81

                         90       100
                 ....*....|....*....|....*
gi 488332510  84 GEYIAEFESDDYVALHAYERLYNTA 108
Cdd:COG1216   82 GDYLLFLDDDTVVEPDWLERLLAAA 106

Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 325 KVSVVMPIHNASKYLRQTLETVCEQSLREIEIILVENGSTDNTMDIINEFavKDPRITGISIGKS-NPGHARNVGISMAR 403
Cdd:COG1216    4 KVSVVIPTYNRPELLRRCLESLLAQTYPPFEVIVVDNGSTDGTAELLAAL--AFPRVRVIRNPENlGFAAARNLGLRAAG 81

                         90
                 ....*....|....*....
gi 488332510 404 GRYLQFLDADDHFEANLLQ 422
Cdd:COG1216   82 GDYLLFLDDDTVVEPDWLE 100

ExoA is involved in the biosynthesis of succinoglycan; Succinoglycan Biosynthesis Protein ExoA catalyzes the formation of a beta-1,3 linkage of the second sugar (glucose) of the succinoglycan with the galactose on the lipid carrie. Succinoglycan is an acidic exopolysaccharide that is important for invasion of the nodules. Succinoglycan is a high-molecular-weight polymer composed of repeating octasaccharide units. These units are synthesized on membrane-bound isoprenoid lipid carriers, beginning with galactose followed by seven glucose molecules, and modified by the addition of acetate, succinate, and pyruvate. ExoA is a membrane protein with a transmembrance domain at c-terminus.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 325 KVSVVMPIHNASKYLRQTLETVCEQS--LREIEIILVENGSTDNTMDIINEFAVKDPRITGISIGKSNPGHARNVGISMA 402
Cdd:cd02525    1 FVSIIIPVRNEEKYIEELLESLLNQSypKDLIEIIVVDGGSTDGTREIVQEYAAKDPRIRLIDNPKRIQSAGLNIGIRNS 80

                         90       100
                 ....*....|....*....|
gi 488332510 403 RGRYLQFLDADDHFEANLLQ 422
Cdd:cd02525   81 RGDIIIRVDAHAVYPKDYIL 100

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   8 VIVPVYNVETYLEEALMSLKNQTLKEI--EFLIINDGSTDNSQKIIEEIAQDDPRFRVF-NVKNGGIGKAFNLGVSEAKG 84
Cdd:cd04179    1 VVIPAYNEEENIPELVERLLAVLEEGYdyEIIVVDDGSTDGTAEIARELAARVPRVRVIrLSRNFGKGAAVRAGFKAARG 80

                         90       100       110
                 ....*....|....*....|....*....|..
gi 488332510  85 EYIAEFESDDYVALHAYERLYNTAKSHHADVV 116
Cdd:cd04179   81 DIVVTMDADLQHPPEDIPKLLEKLLEGGADVV 112

Myxococcus xanthus RfbC like proteins are required for O-antigen biosynthesis; The rfbC gene encodes a predicted protein of 1,276 amino acids, which is required for O-antigen biosynthesis in Myxococcus xanthus. It is a subfamily of Glycosyltransferase Family GT2, which includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 325 KVSVVMPIHNAS-KYLRQTLETVCEQSLREIEIILVENGSTDN-TMDIINEFAVKDPRITgISIGKSNpGH---ARNVGI 399
Cdd:cd04184    2 LISIVMPVYNTPeKYLREAIESVRAQTYPNWELCIADDASTDPeVKRVLKKYAAQDPRIK-VVFREEN-GGisaATNSAL 79

                         90       100
                 ....*....|....*....|..
gi 488332510 400 SMARGRYLQFLDADDHFEANLL 421
Cdd:cd04184   80 ELATGEFVALLDHDDELAPHAL 101

WfgS and WfeV are involved in O-antigen biosynthesis; Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   7 SVIVPVYNVETYLEEALMSLKNQTLKEIEFLIINDGSTDNSQKIIEEIAqdDPRFRVFNVKNGGIGKAFNLGVSEAKGEY 86
Cdd:cd06433    1 SIITPTYNQAETLEETIDSVLSQTYPNIEYIVIDGGSTDGTVDIIKKYE--DKITYWISEPDKGIYDAMNKGIALATGDI 78

                         90       100       110
                 ....*....|....*....|....*....|.
gi 488332510  87 IAEFESDDYVALHA-YERLYNTAKSHHADVV 116
Cdd:cd06433   79 IGFLNSDDTLLPGAlLAVVAAFAEHPEVDVV 109

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 327 SVVMPIHNASKYLRQTLETVCEQSLREIEIILVENGSTDNTMDIINEFAVKDPRITGISIGKSNPGHARNV--GISMARG 404
Cdd:cd04196    1 AVLMATYNGEKYLREQLDSILAQTYKNDELIISDDGSTDGTVEIIKEYIDKDPFIIILIRNGKNLGVARNFesLLQAADG 80

                         90
                 ....*....|
gi 488332510 405 RYLQFLDADD 414
Cdd:cd04196   81 DYVFFCDQDD 90

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 328 VVMPIHNASKYLRQTLETVCEQSLREI--EIILVENGSTDNTMDIINEFAVKDPRITGISIGKsNPGHARNV--GISMAR 403
Cdd:cd04179    1 VVIPAYNEEENIPELVERLLAVLEEGYdyEIIVVDDGSTDGTAEIARELAARVPRVRVIRLSR-NFGKGAAVraGFKAAR 79

                         90
                 ....*....|
gi 488332510 404 GRYLQFLDAD 413
Cdd:cd04179   80 GDIVVTMDAD 89

CESA_like is the cellulose synthase superfamily; The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 328 VVMPIHNASKYLRQTLETVCEQSLREIEIILVENGSTDNTMDIINEFAVKDPRITGISIGKSNPGHAR--NVGISMARGR 405
Cdd:cd06423    1 IIVPAYNEEAVIERTIESLLALDYPKLEVIVVDDGSTDDTLEILEELAALYIRRVLVVRDKENGGKAGalNAGLRHAKGD 80

                         90
                 ....*....|....*..
gi 488332510 406 YLQFLDADDHFEANLLQ 422
Cdd:cd06423   81 IVVVLDADTILEPDALK 97

Bacterial DPM1_like enzymes are related to eukaryotic DPM1; A family of bacterial enzymes related to eukaryotic DPM1; Although the mechanism of eukaryotic enzyme is well studied, the mechanism of the bacterial enzymes is not well understood. The eukaryotic DPM1 is the catalytic subunit of eukaryotic Dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. The enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. This protein family belongs to Glycosyltransferase 2 superfamily.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   8 VIVPVYNVETYLEE---ALMSLKNQTLKEIEFLIINDGSTDNSQKIIEEIAQDDPRFRVF-NVKNGGIGKAFNLGVSEAK 83
Cdd:cd04187    1 IVVPVYNEEENLPElyeRLKAVLESLGYDYEIIFVDDGSTDRTLEILRELAARDPRVKVIrLSRNFGQQAALLAGLDHAR 80

                         90
                 ....*....|
gi 488332510  84 GEYIAEFESD 93
Cdd:cd04187   81 GDAVITMDAD 90

DPG_synthase is involved in protein N-linked glycosylation; UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 328 VVMPIHNASKYLRQTLETVCE----QSLREIEIILVENGSTDNTMDIINEFAVKDPRITGIS-----IGKsnpGHARNVG 398
Cdd:cd04188    1 VVIPAYNEEKRLPPTLEEAVEyleeRPSFSYEIIVVDDGSKDGTAEVARKLARKNPALIRVLtlpknRGK---GGAVRAG 77

                         90       100
                 ....*....|....*....|....
gi 488332510 399 ISMARGRYLQFLDAD-----DHFE 417
Cdd:cd04188   78 MLAARGDYILFADADlatpfEELE 101

UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide; UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 325 KVSVVMPIHNASKYLRQTLEtvceqSLREI--EIILVENGSTDNTMDIINEFAVKdpritGISIGKSNPGHARNVGISMA 402
Cdd:cd02511    1 TLSVVIITKNEERNIERCLE-----SVKWAvdEIIVVDSGSTDRTVEIAKEYGAK-----VYQRWWDGFGAQRNFALELA 70

                         90       100
                 ....*....|....*....|
gi 488332510 403 RGRYLQFLDADDHFEANLLQ 422
Cdd:cd02511   71 TNDWVLSLDADERLTPELAD 90

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510 328 VVMPIHNASKYLRQTLETVCEQSLREIEIILVENGSTDNTMDIINEFAvkdPRITGISIGKS-NPGHARNVGISMARGRY 406
Cdd:cd04186    1 IIIVNYNSLEYLKACLDSLLAQTYPDFEVIVVDNASTDGSVELLRELF---PEVRLIRNGENlGFGAGNNQGIREAKGDY 77

                         90
                 ....*....|....*....
gi 488332510 407 LQFLDADDHFEANLLQDAY 425
Cdd:cd04186   78 VLLLNPDTVVEPGALLELL 96

GT2_AmsE_like is involved in exopolysaccharide amylovora biosynthesis; AmsE is a glycosyltransferase involved in exopolysaccharide amylovora biosynthesis in Erwinia amylovora. Amylovara is one of the three exopolysaccharide produced by E. amylovora. Amylovara-deficient mutants are non-pathogenic. It is a subfamily of Glycosyltransferase Family GT2, which includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   7 SVIVPVYNVET--YLEEALMSLKNQTLKEIEFLIINDGS-TDNSQKIIEEIAQDDPrFRVFNV-KNGGIGKAFNLGVSEA 82
Cdd:cd04195    1 SVLMSVYIKEKpeFLREALESILKQTLPPDEVVLVKDGPvTQSLNEVLEEFKRKLP-LKVVPLeKNRGLGKALNEGLKHC 79

                         90
                 ....*....|..
gi 488332510  83 KGEYIAEFESDD 94
Cdd:cd04195   80 TYDWVARMDTDD 91

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   8 VIVPVYNVETYLEEALMSLKNQTL--KEIEFLIINDGSTDNSQKIIEEIAQ-DDPRFRVFNVKNGGI-GKAFNL--GVSE 81
Cdd:cd04192    1 VVIAARNEAENLPRLLQSLSALDYpkEKFEVILVDDHSTDGTVQILEFAAAkPNFQLKILNNSRVSIsGKKNALttAIKA 80

                         90
                 ....*....|..
gi 488332510  82 AKGEYIAEFESD 93
Cdd:cd04192   81 AKGDWIVTTDAD 92

DPM1_like represents putative enzymes similar to eukaryotic DPM1; Proteins similar to eukaryotic DPM1, including enzymes from bacteria and archaea; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. This protein family belongs to Glycosyltransferase 2 superfamily.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488332510   8 VIVPVYNvE----TYLEEALMSlknqTLKEIEF--LIINDGSTDNSQKIIEEIAQDDPRFRV-FNVKNGGIGKAFNLGVS 80
Cdd:cd06442    1 IIIPTYN-EreniPELIERLDA----ALKGIDYeiIVVDDNSPDGTAEIVRELAKEYPRVRLiVRPGKRGLGSAYIEGFK 75

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 488332510  81 EAKGEYIAEFESD-----DYVAlhayeRLYNTAKSHHADVV 116
Cdd:cd06442   76 AARGDVIVVMDADlshppEYIP-----ELLEAQLEGGADLV 111