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Conserved domains on  [gi|488397600|ref|WP_002466985|]
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MULTISPECIES: NAD(P)H-dependent oxidoreductase [Staphylococcus]

Protein Classification

NAD(P)H-dependent oxidoreductase( domain architecture ID 10114874)

NAD(P)H-dependent oxidoreductase, similar to nitroreductase NfsB, which catalyzes the reduction of flavin or nitrocompounds using NAD(P)H as electron donor

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 7-215 3.06e-74

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


:

Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 221.36  E-value: 3.06e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   7 TIIDAFHFRHATKQFDASKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGAQKQLDSASHFMLIF 86
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAAWFNQPQIKDASHVVVFL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  87 ARKnvtsqspyvqnlvknvkgyeestipavykkyddfqtefhindnertlyDWACKQTYIALGNMMTSAALLGIDSCPIE 166
Cdd:cd02149   81 AKK------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSCPIE 112

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 488397600 167 GFDVDTVTQILSdegiLDTENFGISVMAAFGYRAgEPPHGKVRQTQDDV 215
Cdd:cd02149  113 GFDPAKLDEILG----LDEKGYKISVMVAFGYRS-EEKLPKSRKPLEDV 156
 
Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 7-215 3.06e-74

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 221.36  E-value: 3.06e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   7 TIIDAFHFRHATKQFDASKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGAQKQLDSASHFMLIF 86
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAAWFNQPQIKDASHVVVFL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  87 ARKnvtsqspyvqnlvknvkgyeestipavykkyddfqtefhindnertlyDWACKQTYIALGNMMTSAALLGIDSCPIE 166
Cdd:cd02149   81 AKK------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSCPIE 112

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 488397600 167 GFDVDTVTQILSdegiLDTENFGISVMAAFGYRAgEPPHGKVRQTQDDV 215
Cdd:cd02149  113 GFDPAKLDEILG----LDEKGYKISVMVAFGYRS-EEKLPKSRKPLEDV 156
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 8-216 3.73e-41

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 137.68  E-value: 3.73e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   8 IIDAFHFRHATKQFDaSKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKP-YSWGAQKQLDSASHFMLIF 86
Cdd:COG0778    1 LLELLLTRRSVRKFT-DKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEaLAEANQEWVADAPVLIVVC 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  87 ARKnvtsqspyvqnlvknvkgyeestipavykkyddfqtefhiNDNERTLYDWACKQTYIALGNMMTSAALLGIDSCPIE 166
Cdd:COG0778   80 ADP----------------------------------------DRSEKVPERYALLDAGIAAQNLLLAARALGLGTCWIG 119

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 488397600 167 GFDVDTVTQILsdeGIldTENFGISVMAAFGYRAgEPPHGKVRQTQDDVV 216
Cdd:COG0778  120 GFDPEKVRELL---GL--PEGEEPVALLALGYPA-EELNPRPRKPLEEVV 163
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
4-202 6.18e-33

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 118.15  E-value: 6.18e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   4 MNqtIIDAFHFRHATKQFDASKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKP-----YSWGAQKQLDs 78
Cdd:PRK11053   1 MD--IVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKaaagnYAFNERKILD- 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  79 ASHFMLIFARKNVTSQspYVQNLVKNvkgyEE-------STIPAVYKKYDDFQTEFHINDNeRTLYDWACKQTYIALGNM 151
Cdd:PRK11053  78 ASHVVVFCAKTDMDDA--YLELVLEQ----EDadgrfatEEAKAAQDKGRRFFADMHRKEL-KDLQHWMEKQVYLALGNL 150

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 488397600 152 MTSAALLGIDSCPIEGFDVDtvtqILSDEGILDTENFGISVMAAFGYRAGE 202
Cdd:PRK11053 151 LLGAAALGIDATPIEGFDAA----ILDAEFGLREKGLTSSVVVPLGYHSEE 197
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 15-198 1.43e-29

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 107.86  E-value: 1.43e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   15 RHATKQFDASKtISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGAQKQLDSASHFMLIFARKNvtsq 94
Cdd:pfam00881   4 RRSVRKFDPEP-VPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLLLRRDAN---- 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   95 spyvQNLVKNVKGYEESTIPAVYKKYDDFqtefhINDNERTLYDWACKQTYIALGNMMTSAALLGIDSCPIEGFDVDTVT 174
Cdd:pfam00881  79 ----LKLLLQDFLRGAPVLIVITASLSTY-----LRKAAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAAAVR 149

                         170       180
                  ....*....|....*....|....
gi 488397600  175 QILsdeGIldTENFGISVMAAFGY 198
Cdd:pfam00881 150 ELL---GL--PDDERLVGLIAVGY 168
 
Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 7-215 3.06e-74

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 221.36  E-value: 3.06e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   7 TIIDAFHFRHATKQFDASKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGAQKQLDSASHFMLIF 86
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAAWFNQPQIKDASHVVVFL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  87 ARKnvtsqspyvqnlvknvkgyeestipavykkyddfqtefhindnertlyDWACKQTYIALGNMMTSAALLGIDSCPIE 166
Cdd:cd02149   81 AKK------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSCPIE 112

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 488397600 167 GFDVDTVTQILSdegiLDTENFGISVMAAFGYRAgEPPHGKVRQTQDDV 215
Cdd:cd02149  113 GFDPAKLDEILG----LDEKGYKISVMVAFGYRS-EEKLPKSRKPLEDV 156
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 8-216 3.73e-41

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 137.68  E-value: 3.73e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   8 IIDAFHFRHATKQFDaSKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKP-YSWGAQKQLDSASHFMLIF 86
Cdd:COG0778    1 LLELLLTRRSVRKFT-DKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEaLAEANQEWVADAPVLIVVC 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  87 ARKnvtsqspyvqnlvknvkgyeestipavykkyddfqtefhiNDNERTLYDWACKQTYIALGNMMTSAALLGIDSCPIE 166
Cdd:COG0778   80 ADP----------------------------------------DRSEKVPERYALLDAGIAAQNLLLAARALGLGTCWIG 119

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 488397600 167 GFDVDTVTQILsdeGIldTENFGISVMAAFGYRAgEPPHGKVRQTQDDVV 216
Cdd:COG0778  120 GFDPEKVRELL---GL--PEGEEPVALLALGYPA-EELNPRPRKPLEEVV 163
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
4-202 6.18e-33

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 118.15  E-value: 6.18e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   4 MNqtIIDAFHFRHATKQFDASKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKP-----YSWGAQKQLDs 78
Cdd:PRK11053   1 MD--IVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKaaagnYAFNERKILD- 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  79 ASHFMLIFARKNVTSQspYVQNLVKNvkgyEE-------STIPAVYKKYDDFQTEFHINDNeRTLYDWACKQTYIALGNM 151
Cdd:PRK11053  78 ASHVVVFCAKTDMDDA--YLELVLEQ----EDadgrfatEEAKAAQDKGRRFFADMHRKEL-KDLQHWMEKQVYLALGNL 150

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 488397600 152 MTSAALLGIDSCPIEGFDVDtvtqILSDEGILDTENFGISVMAAFGYRAGE 202
Cdd:PRK11053 151 LLGAAALGIDATPIEGFDAA----ILDAEFGLREKGLTSSVVVPLGYHSEE 197
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 15-198 1.43e-29

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 107.86  E-value: 1.43e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   15 RHATKQFDASKtISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGAQKQLDSASHFMLIFARKNvtsq 94
Cdd:pfam00881   4 RRSVRKFDPEP-VPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLLLRRDAN---- 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   95 spyvQNLVKNVKGYEESTIPAVYKKYDDFqtefhINDNERTLYDWACKQTYIALGNMMTSAALLGIDSCPIEGFDVDTVT 174
Cdd:pfam00881  79 ----LKLLLQDFLRGAPVLIVITASLSTY-----LRKAAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAAAVR 149

                         170       180
                  ....*....|....*....|....
gi 488397600  175 QILsdeGIldTENFGISVMAAFGY 198
Cdd:pfam00881 150 ELL---GL--PDDERLVGLIAVGY 168
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 10-215 5.78e-29

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 105.79  E-value: 5.78e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  10 DAFHFRHATKQFDASKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGaQKQLDSASHFMLIFARK 89
Cdd:cd02137    2 EVIKSRRSVRNFDPDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAAYN-QPQVTTASAVILVLGDL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  90 NVTsqspyvqnlvknvkgyeestipavykkyddfqtefhindnertlydwackqtyIALGNMMTSAALLGIDSCPIEGFD 169
Cdd:cd02137   81 NAG-----------------------------------------------------LAAMNLMLAAKAKGYDTCPMGGFD 107

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 488397600 170 VDTVTQILSDEgildtENFGISVMAAFGYRAGEPPHGKvRQTQDDV 215
Cdd:cd02137  108 KEKVAELLNLP-----DRYVPVLLIAIGKAADKAPRSG-RLPVDEV 147
nitroreductase cd03370
uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus ...
 10-205 3.34e-20

uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus thermophilus NADH oxidase and other, uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380327 [Multi-domain]  Cd Length: 191  Bit Score: 84.29  E-value: 3.34e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  10 DAFHFRHATKQFdASKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGaQKQLDSASHFMLIF--- 86
Cdd:cd03370    3 EAIESRRSIRKY-TQEPVPDEDLREILRLAGLAPSAWNIQPWRFVVVRDAELKEQLQAAAYG-QAQVTSAPAVIVIYsdm 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  87 ----ARKNVTSQsPYVQnlvKNVKGYEESTIPAVYKKYDDFQTEfhindnertlyDWACKQTYIALGNMMTSAALLGIDS 162
Cdd:cd03370   81 edalANLEETIH-PGLS---EERRQREAAGLRGAFGKMSVEQRG-----------QWGLAQANIALGFLLLAAQSLGYDT 145

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 488397600 163 CPIEGFDVDTVTQILsdeGIldTENFGISVMAAFGYRA--GEPPH 205
Cdd:cd03370  146 SPMLGFDPEKVKALL---GL--PEHVTIAALVALGKPAeeGYPHH 185
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 15-198 1.89e-18

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 78.11  E-value: 1.89e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  15 RHATKQFDaSKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGAQKQLDSASHFMLIFArknvtsq 94
Cdd:cd02062    4 RRSIRKFT-DKPVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDREKKEKLAKLAAPNQKFIAGAPVVIVVVA------- 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  95 spyvqnlvknvkgyeestipavykkyddfqtefhinDNERTLYDWACKqTYIALGNMMTSAALLGIDSCPIEGFDV--DT 172
Cdd:cd02062   76 ------------------------------------DPDKSRPWALED-AGAAAQNLLLAAAALGLGSCWIGGFDFreDK 118

                        170       180
                 ....*....|....*....|....*.
gi 488397600 173 VTQILsdeGIldTENFGISVMAAFGY 198
Cdd:cd02062  119 VRELL---GI--PENLRPVALIAIGY 139
TdsD-like cd02138
nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase ...
 15-205 2.47e-14

nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to Burkholderia pseudomallei TdsD, may be involved in the processing of organosulfur compounds. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380315 [Multi-domain]  Cd Length: 174  Bit Score: 67.95  E-value: 2.47e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  15 RHATKQFDASKtISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKT-----LRDKLKPY--SWgAQKqldsASHFMLIFA 87
Cdd:cd02138    5 RWSPRAFSPEP-ISEEDLLSLFEAARWAPSCFNEQPWRFVVARRDTeafekLLDLLAEGnqSW-AKN----APVLIVVLA 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  88 RKNvtsqspyvqnlvknvkgYEESTIPAVYKKYDdfqtefhindnertlydwackqTYIALGNMMTSAALLGIDSCPIEG 167
Cdd:cd02138   79 KTE-----------------FDHNGKPNRYALFD----------------------TGAAVANLALQATALGLVVHQMAG 119

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 488397600 168 FDVDTVTQILsdeGIldTENFGISVMAAFGYRaGEPPH 205
Cdd:cd02138  120 FDPEKAKEAL---GI--PDEYEPITMIAIGYP-GDPES 151
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 15-219 9.06e-14

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 66.07  E-value: 9.06e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  15 RHATKQFDaSKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTlRDKLKPYSWGAQkqldsasHFMLIFARKnvtsq 94
Cdd:cd02136    5 RRSVRAFK-DKPVPKETIEKILEAARRAPSGKNTQPWRVYVVTGKA-RERLKKAFFGAP-------VALFLTMDK----- 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  95 spyvqnlvknvkgyeestipavykkyddfqtefhiNDNERTLYDWACkqtyiALGNMMTSAALLGIDSCPIeGFDVDTVT 174
Cdd:cd02136   71 -----------------------------------VLGPWSWFDLGA-----FLQNLMLAAHALGLGTCPQ-GALAGYPD 109

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 488397600 175 QILSDEGILDTENFGISVmaAFGYRAGEPPHGKVRQTQDDVVKWI 219
Cdd:cd02136  110 VVRKELGIPDDEELVCGI--ALGYPDPDAPVNQFRTPREPLEEFV 152
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 27-218 9.41e-14

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 66.34  E-value: 9.41e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  27 ISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGAqkqldsasHFMLifarknvtsQSPYVqnLVknVK 106
Cdd:cd02139   19 VEEEKLLRILEAARLAPSAKNRQPWRFIVVKDKELKEKLAEAANGQ--------KFIA---------EAPVV--IV--AC 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600 107 GYEESTIPAVYKKYddfqteFHIndnertlyDWAckqtyIALGNMMTSAALLGIDSCPIEGFDVDTVTQILsdeGIldTE 186
Cdd:cd02139   78 ADPSESGMGCGKPY------YLV--------DVA-----IAMEHLVLAATEEGLGTCWIGAFDEDKVKEIL---GI--PE 133

                        170       180       190
                 ....*....|....*....|....*....|..
gi 488397600 187 NFGISVMAAFGYRAGEPPHgKVRQTQDDVVKW 218
Cdd:cd02139  134 EYRVVALTPLGYPAEEPPP-RPRKPLEEIVFY 164
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 10-198 6.30e-13

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 63.51  E-value: 6.30e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  10 DAFHFRHATKQFdASKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGAQKQLDSAshfmlifark 89
Cdd:cd20608    2 EAIKTRRSVRRF-SDKPVEEEKLEKILEAARLAPSWANKQCWRFIVVTDKETLSELAKKESPSNGWLKDA---------- 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  90 nvtsqsPYVqnLVKNVKGYEESTipavykkyddfqtefhINDNERTLYDWAckqtyIALGNMMTSAALLGIDSCPIEGFD 169
Cdd:cd20608   71 ------PVI--IVVCADPKDSGW----------------LNGQNYYLVDAA-----IAMQNLMLAATDLGLGTCWIGAFD 121

                        170       180
                 ....*....|....*....|....*....
gi 488397600 170 VDTVTQILsdeGIldTENFGISVMAAFGY 198
Cdd:cd20608  122 EKKVKEIL---GI--PENIRVVALTPLGY 145
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 15-178 3.85e-10

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 56.24  E-value: 3.85e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  15 RHATKQFDaSKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLK---PYSWGAQKqldsashFMLIFARKNV 91
Cdd:cd20609    9 RYSVRKFS-DKPVEKEKLDKILEAGRLAPTAVNYQPQRILVVRSEEALEKLAkatPRFFGAPL-------VIVVCYDKDE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  92 TSQSPYvqnlvknvKGYEESTIPAVykkyddfqtefhindnertlydwackqtyIALGNMMTSAALLGIDSCPIEGFDVD 171
Cdd:cd20609   81 SWKRPY--------DGKDSGDIDAA-----------------------------IVATHMMLAATELGLGTCWVGNFDPE 123


                 ....*..
gi 488397600 172 TVTQILS 178
Cdd:cd20609  124 KVREAFN 130
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 15-204 1.11e-08

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 52.53  E-value: 1.11e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  15 RHATKQFDASKtISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSWGAQKQLDSASHFMLIFARKNvtsq 94
Cdd:cd02151    6 RRSIRKYTDEP-IEEEKLEEILEAALLAPSSRNSRPVEFIVVDDKETLKKLSECKPHGSAFLKGAPAAIVVLADTE---- 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600  95 spyvqnlvknvkgyeestipavykkyddfQTEFHINDnertlydwackqTYIALGNMMTSAALLGIDSCPIEGFDVDTVT 174
Cdd:cd02151   81 -----------------------------KSDTWIED------------ASIAATYIQLAAESLGLGSCWIQIRNRETQD 119

                        170       180       190
                 ....*....|....*....|....*....|....
gi 488397600 175 QILSDE---GILDT-ENFGISVMAAFGYRAGEPP 204
Cdd:cd02151  120 GKTAEEyvrELLGIpENYRVLCIIALGYPDEEKP 153
nitroreductase cd02150
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 24-97 2.58e-08

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380325 [Multi-domain]  Cd Length: 156  Bit Score: 51.45  E-value: 2.58e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 488397600  24 SKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLK---PYSwgaqKQLDSASHFMLIFARKNVTSQSPY 97
Cdd:cd02150   12 DKPVEEEDIEKLLRAAMAAPSAGNQQPWHFIVVTDREKLDKIAeahPYG----KMLKEAPLAIVVCGDPSKEKAPGY 84
nitroreductase cd20610
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 15-59 2.65e-05

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380331 [Multi-domain]  Cd Length: 167  Bit Score: 43.04  E-value: 2.65e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 488397600  15 RHATKQFdASKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSK 59
Cdd:cd20610    4 RRSIRKF-KPDPVPKEDIEKILEAANWAPSGMNRQNWEFVVVKGG 47
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 25-86 3.06e-05

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 43.38  E-value: 3.06e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 488397600  25 KTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSwGAQKQLDSASHFmLIF 86
Cdd:cd02146   17 EPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELA-GNQPYVAQAPVF-LVF 76
YdjA-like cd02135
nitroreductase family protein similar to Escherichia coli YdjA; A subfamily of the ...
 9-65 7.40e-05

nitroreductase family protein similar to Escherichia coli YdjA; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase YdjA from Escherichia coli. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. Members of this family are also called NADH dehydrogenase, oxygen-insensitive NAD(P)H nitrogenase or dihydropteridine reductase.


Pssm-ID: 380312 [Multi-domain]  Cd Length: 162  Bit Score: 41.82  E-value: 7.40e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 488397600   9 IDAFHFRHATKQFDASKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTlRDKL 65
Cdd:cd02135    1 LELIKTRRSIRKFKLTGAPPEEQLEELLEAAMWAPNHGKLEPWRFIVVTGEG-RERL 56
TM1586_NiRdase pfam14512
Putative TM nitroreductase; Compared with the more traditional NADH oxidase/flavin reductase ...
 28-84 3.35e-04

Putative TM nitroreductase; Compared with the more traditional NADH oxidase/flavin reductase family, this family is a duplication, consisting of two similar domains arranged as the subunits of the dimeric NADH oxidase/flavin reductase with one conserved active site.


Pssm-ID: 405236 [Multi-domain]  Cd Length: 214  Bit Score: 40.35  E-value: 3.35e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   28 SNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRdkLKPYSWGAQKQLD---SASHFML 84
Cdd:pfam14512 155 LPEWFKEGMEAARLAPSAMNQQPWRFVLVDDNRIH--AFASVIGFFSKIDlgiAKSHFEL 212
PRK10765 PRK10765
oxygen-insensitive NADPH nitroreductase;
1-86 1.13e-03

oxygen-insensitive NADPH nitroreductase;


Pssm-ID: 182710  Cd Length: 240  Bit Score: 38.80  E-value: 1.13e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488397600   1 MSDMNQTIIDAFHFRHATKQfdaskTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDKLKPYSwGAQKQLDSAS 80
Cdd:PRK10765   1 MTPTIELILSHRSIRHFTDE-----PISEAQREAIINAARAASSSSFLQCSSIIRITDKALREALVELT-GGQKYVAQAA 74


                 ....*.
gi 488397600  81 HFmLIF 86
Cdd:PRK10765  75 EF-WVF 79
iodotyrosine_dehalogenase cd02144
iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the ...
 24-64 2.52e-03

iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the 3, 5 positions of L-tyosine in thyroid, liver and kidney, using NADPH as electron donor. This enzyme is a homolog of the nitroreductase family. These enzymes are usually homodimers.


Pssm-ID: 380320  Cd Length: 192  Bit Score: 37.52  E-value: 2.52e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 488397600  24 SKTISNEDFETILEAGRLSPSSLGLEPWKFVVVQSKTLRDK 64
Cdd:cd02144   16 SEPVPREVIENAIRTAGTAPSGANTQPWTFVVVSDPEIKRK 56
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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