Domain of unknown function (DUF3470); This presumed domain is functionally uncharacterized. This domain is found in bacteria. This domain is about 50 amino acids in length. This domain is found associated with pfam00037. This domain has a single completely conserved residue N that may be functionally important.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 488617843   66 ENFIELNAELAEVWPNITEKKDAMPDAAEWDGKTGKIADLER 107
Cdd:pfam11953   1 EKFIELNAELAKIWPNITEKKDPLPDADEWKGVPDKLELLER 42

Domain of unknown function (DUF3470); This presumed domain is functionally uncharacterized. This domain is found in bacteria. This domain is about 50 amino acids in length. This domain is found associated with pfam00037. This domain has a single completely conserved residue N that may be functionally important.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 488617843   66 ENFIELNAELAEVWPNITEKKDAMPDAAEWDGKTGKIADLER 107
Cdd:pfam11953   1 EKFIELNAELAKIWPNITEKKDPLPDADEWKGVPDKLELLER 42

formate dehydrogenase FDH3 subunit beta; Members of this family are the beta subunit of the FDH3 type of formate dehydrogenase as found in Methylorubrum (Methylobacterium) extorquens.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 488617843   9 CIKCKYTDCVEVCPVDCFYEGPNFLVIH-PDECIDCALCEPECP 51
Cdd:NF038355  54 CMHCTDAPCAAVCPVDCFYIRADGIVLHdKDKCIGCGYCLYACP 97

Beta subunit of the DMSO Reductase (DMSOR) family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 488617843   3 FVVTDNCIKCKYTDCVEVCPVDCFYEGPN-FLVIHPDECIDCALCEPECPAVAIY 56
Cdd:cd04410   44 AFLPVSCMHCEDPPCVKACPTGAIYKDEDgIVLIDEDKCIGCGSCVEACPYGAIV 98

2-oxoglutarate-acceptor oxidoreductase subunit OorD; Reviewed

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 488617843  11 KCKYTD-CVEVCPVDCF---YEGPNFL-----VIHPDECIDCALCEPECPAVAIYSED 59
Cdd:PRK09626  17 RCKACDiCVSVCPAGVLamrIDPHAVLgkmikVVHPESCIGCRECELHCPDFAIYVAD 74

4Fe-4S dicluster domain; Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 488617843    9 CIKCKYtdCVEVCPVDCFY-------EGPNFLVIHPDECIDCALCEPECP 51
Cdd:pfam12838   1 CIGCGA--CVAACPVGAITldevgekKGTKTVVIDPERCVGCGACVAVCP 48

Domain of unknown function (DUF3470); This presumed domain is functionally uncharacterized. This domain is found in bacteria. This domain is about 50 amino acids in length. This domain is found associated with pfam00037. This domain has a single completely conserved residue N that may be functionally important.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 488617843   66 ENFIELNAELAEVWPNITEKKDAMPDAAEWDGKTGKIADLER 107
Cdd:pfam11953   1 EKFIELNAELAKIWPNITEKKDPLPDADEWKGVPDKLELLER 42

TPP-dependent indolepyruvate ferredoxin oxidoreductase, alpha subunit [Energy production and conversion];

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 488617843   1 MTFVV-TDNCIKCKytDCVEVCPVDCFYEGPNFLVIHPDECIDCALCEPECPAVAIYSEDEIPA 63
Cdd:COG4231   15 MRYVIdEDKCTGCG--ACVKVCPADAIEEGDGKAVIDPDLCIGCGSCVQVCPVDAIKLEKRVPE 76

Pyruvate:ferredoxin oxidoreductase or related 2-oxoacid:ferredoxin oxidoreductase, delta subunit [Energy production and conversion]; Pyruvate:ferredoxin oxidoreductase or related 2-oxoacid:ferredoxin oxidoreductase, delta subunit is part of the Pathway/BioSystem: Pyruvate oxidation

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 488617843   7 DNCIKCKytDCVEVCPVDCFYEGPN-FLVIHPDECIDCALCEPECPAVAIYSEDE 60
Cdd:COG1144   30 DKCIGCG--LCWIVCPDGAIRVDDGkYYGIDYDYCKGCGICAEVCPVKAIEMVPE 82

Dissimilatory sulfite reductase (desulfoviridin), alpha and beta subunits [Inorganic ion transport and metabolism];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 488617843   4 VVTDNCIKCKytDCVEVCPVDCFYEGPNFLVIHPDECIDCALCEPECPAVAIYSE 58
Cdd:COG2221   12 IDEEKCIGCG--LCVAVCPTGAISLDDGKLVIDEEKCIGCGACIRVCPTGAIKGE 64

Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I) [Energy production and conversion]; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I) is part of the Pathway/BioSystem: NADH dehydrogenase

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 488617843   6 TDNCIKCKytDCVEVCPVDCFY----EGPNFLVIHPDECIDCALCEPECPAVAIY 56
Cdd:COG1143    1 EDKCIGCG--LCVRVCPVDAITiedgEPGKVYVIDPDKCIGCGLCVEVCPTGAIS 53

formate dehydrogenase FDH3 subunit beta; Members of this family are the beta subunit of the FDH3 type of formate dehydrogenase as found in Methylorubrum (Methylobacterium) extorquens.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 488617843   9 CIKCKYTDCVEVCPVDCFYEGPNFLVIH-PDECIDCALCEPECP 51
Cdd:NF038355  54 CMHCTDAPCAAVCPVDCFYIRADGIVLHdKDKCIGCGYCLYACP 97

Iron only hydrogenase large subunit, C-terminal domain [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 488617843   7 DNCIKCkyTDCVEVCPVDCFYEGPNFLVIHPDECIDCALCEPECPAVAIYSEDEI 61
Cdd:COG4624   91 EKCKNC--YPCVRACPVKAIKVDDGKAEIDEEKCISCGQCVAVCPFGAITEKSDI 143

Beta subunit of the DMSO Reductase (DMSOR) family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 488617843   3 FVVTDNCIKCKYTDCVEVCPVDCFYEGPN-FLVIHPDECIDCALCEPECPAVAIY 56
Cdd:cd04410   44 AFLPVSCMHCEDPPCVKACPTGAIYKDEDgIVLIDEDKCIGCGSCVEACPYGAIV 98

bacterial and archaeal subfamily of SIMIBI; Uncharacterized bacterial and archaeal subfamily of SIMIBI superfamily. Proteins in this superfamily contain an ATP-binding domain and use energy from hydrolysis of ATP to transfer electron or ion. The specific function of this family is unknown.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 488617843   3 FVVTDNCIKCKytDCVEVCPVDCFYEGPNFLVIHPDECIDCALCEPECPAVAIYSEDE 60
Cdd:cd03110   60 FIDQEKCIRCG--NCERVCKFGAILEFFQKLIVDESLCEGCGACVIICPRGAIYLKDR 115

electron transport complex subunit RsxB;

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 488617843   9 CIKCkyTDCVEVCPVDCFYEGPNFL-VIHPDECIDCALCEPECPAVAIyseDEIPAGME 66
Cdd:PRK06991  87 CIGC--TLCMQACPVDAIVGAPKQMhTVLADLCTGCDLCVPPCPVDCI---DMVPVTGE 140

EFR1 family ferrodoxin; Members of the family have a C-terminal ferrodoxin domain, with eight conserved Cys residues in two CxxCxxCxxxCP motifs, each of which binds a 4Fe-4S cluster. The N-terminal region resembles flavodoxin domains, with some members of the family recognized by Pfam models PF12724 (Flavodoxin_5) or PF00258 (Flavodoxin_1).

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 488617843   3 FVVTDNCIKCKYtdCVEVCPVDC--FYEG-PNFLvihpDECIDCALCEPECPAVAI 55
Cdd:NF038196 181 FHVTDKCIGCGI--CAKVCPVNNieMEDGkPVWG----HNCTHCLACIHRCPKEAI 230

ferredoxin-like protein;

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 488617843   6 TDNCIKCKYTDCVEVCPVDCFYEGPNFLVIHPDE--CIDCALCEPECP 51
Cdd:PRK09898 120 ADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHkrCIGCSACTTACP 167

4Fe-4S dicluster domain; Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 488617843    9 CIKCKYtdCVEVCPVDCFY-------EGPNFLVIHPDECIDCALCEPECP 51
Cdd:pfam12838   1 CIGCGA--CVAACPVGAITldevgekKGTKTVVIDPERCVGCGACVAVCP 48

HycB, HydN and similar proteins; This family includes HycB, the FeS subunit of a membrane-associated formate hydrogenlyase system (FHL-1) in Escherichia coli that breaks down formate, produced during anaerobic fermentation, to H2 and CO2. FHL-1 consists of formate dehydrogenase H (FDH-H) and the hydrogenase 3 complex (Hyd-3). HycB is thought to code for the [4Fe-4S] ferredoxin subunit of hydrogenase 3, which functions as an intermediate electron carrier protein between hydrogenase 3 and formate dehydrogenase. HydN codes for the [4Fe-4S] ferredoxin subunit of FDH-H; a hydN in-frame deletion mutation causes only weak reduction in hydrogenase activity, but loss of more than 60% of FDH-H activity. This pathway is only active at low pH and high formate concentrations, and is thought to provide a detoxification/de-acidification system countering the buildup of formate during fermentation.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|
gi 488617843  17 CVEVCPVDCFYEGPNFLVIHPDECIDCALCEPECPAVAIY 56
Cdd:cd10554   64 CANVCPVGAISQEDGVVQVDEERCIGCKLCVLACPFGAIE 103

Uncharacterized polyferredoxin-like protein; This family contains uncharacterized polyferredoxin protein similar to Methanobacterium thermoautotrophicum MvhB. The mvhB is a gene of the methylviologen-reducing hydrogenase operon. It is predicted to contain 12 [4Fe-4S] clusters, and was therefore suggested to be a polyferredoxin. As a subfamily of the beta subunit of the DMSO Reductase (DMSOR) family, it is predicted to function as electron carrier in the reducing reaction.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 488617843   9 CIKCKYtdCVEVCPVDC---------FYEGPNFLVIHPDECIDCALCEPECPAVAIYSEDEIPA 63
Cdd:cd10549   42 CVFCGA--CVEVCPTGAieltpegkeYVPKEKEAEIDEEKCIGCGLCVKVCPVDAITLEDELEI 103

Heterodisulfide reductase, subunit A (polyferredoxin) [Energy production and conversion];

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 488617843   4 VVTDNCIKCKYtdCVEVCPvdcfYEGPNF---LVIHPDE--CIDCALCEPECPAVAI----YSEDEI 61
Cdd:COG1148  493 VDPEKCTGCGR--CVEVCP----YGAISIdekGVAEVNPalCKGCGTCAAACPSGAIslkgFTDDQI 553

anaerobic glycerol-3-phosphate dehydrogenase subunit C;

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 488617843   7 DNCIKCkyTDCVEVCPV---DCFYEGPNF-------------LVIHP--DECIDCALCEPECP 51
Cdd:PRK11168   7 DSCIKC--TVCTTACPVarvNPLYPGPKQagpdgerlrlkdgALYDEslKYCSNCKRCEVACP 67

beta subunit of formate dehydrogenase O (FDH-O) and similar proteins; This subfamily includes beta subunit of formate dehydrogenase family O (FDH-O), which is highly homologous to formate dehydrogenase N (FDH-N), a member of the DMSO reductase family. In E. coli three formate dehydrogenases are synthesized that are capable of oxidizing formate; Fdh-H, couples formate disproportionation to hydrogen and CO2, and is part of the cytoplasmically oriented formate hydrogenlyase complex, while FDH-N and FDH-O indicate their respective induction after growth with nitrate and oxygen. Little is known about FDH-O, although it shows formate oxidase activity during aerobic growth and is also synthesized during nitrate respiration, similar to FDH-N.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 488617843   7 DNCIKCKYTDCVEVCPVDCFYEGP-NFLVIHPDECIDCALCEPECP 51
Cdd:cd10560   76 DVCKHCTDAGCLEACPTGAIFRTEfGTVYIQPDICNGCGYCVAACP 121

Fe-S cluster domain-containing protein;

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 488617843   9 CIKCKYtdCVEVCPVDCFYEGPNFLVIHPDECIDCALCEPECPAVAI 55
Cdd:PRK07118 215 CIGCGK--CVKACPAGAITMENNLAVIDQEKCTSCGKCVEKCPTKAI 259

3-methyl-2-oxobutanoate dehydrogenase subunit delta;

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 488617843   1 MTFVVTDNCIKCKYtdCVEVCPVDCFYEGPN-FLVIHPDECIDCALCEPECPAVAIYSEDE 60
Cdd:PRK09623  45 MPVVDESKCVKCYI--CWKFCPEPAIYIKEDgYVAIDYDYCKGCGICANECPTKAITMVKE 103

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 488617843   9 CIKCKYTDCVEVCPVDCFYEGPNFLVI-HPDECIDCALCEPECPAVAIYSEDE 60
Cdd:cd16370   53 CRACEDPPCAEACPTGALEPRKGGGVVlDKEKCIGCGNCVKACIVGAIFWDEE 105

Fe-S-cluster-containing dehydrogenase component (DMSO reductase) [Energy production and conversion];

                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 488617843  17 CVEVCPVDCFYEGPNFLVIH-PDECIDCALCEPECP 51
Cdd:COG0437   68 CVKVCPTGATYKREDGIVLVdYDKCIGCRYCVAACP 103