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Conserved domains on  [gi|488976376|ref|WP_002887256|]
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MULTISPECIES: nuclear transport factor 2 family protein [Klebsiella]

Protein Classification

nuclear transport factor 2 family protein( domain architecture ID 10007626)

nuclear transport factor 2 (NTF2) family protein with a SnoaL-like polyketide cyclase domain

CATH:  3.10.450.50
SCOP:  4001306|3000472

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
7-124 7.46e-20

Ketosteroid isomerase-related protein [General function prediction only];


:

Pssm-ID: 442849  Cd Length: 126  Bit Score: 79.20  E-value: 7.46e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376   7 VIRRfveYYAGLDAQPPAALATLYHPDATLSDPF----GQHQGLFAIQRYFTHLLANVEQCRFTIDTPLCDGQRFAVTWT 82
Cdd:COG3631    5 LVRR---FYAAFNAGDVDALLALLAEDVVWEDPGgpgaGTYRGKEAVRAFFARLAAAFEDLRFEVRRVVADGDRVVVEGT 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 488976376  83 MHWSHPriAGGETLALPGCSVVDIAGEQVLHQRDYYDAGEMI 124
Cdd:COG3631   82 VRGTVL--ATGKPYENRYADVFTVRDGKIVRVREYFDTAALA 121
 
Name Accession Description Interval E-value
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
7-124 7.46e-20

Ketosteroid isomerase-related protein [General function prediction only];


Pssm-ID: 442849  Cd Length: 126  Bit Score: 79.20  E-value: 7.46e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376   7 VIRRfveYYAGLDAQPPAALATLYHPDATLSDPF----GQHQGLFAIQRYFTHLLANVEQCRFTIDTPLCDGQRFAVTWT 82
Cdd:COG3631    5 LVRR---FYAAFNAGDVDALLALLAEDVVWEDPGgpgaGTYRGKEAVRAFFARLAAAFEDLRFEVRRVVADGDRVVVEGT 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 488976376  83 MHWSHPriAGGETLALPGCSVVDIAGEQVLHQRDYYDAGEMI 124
Cdd:COG3631   82 VRGTVL--ATGKPYENRYADVFTVRDGKIVRVREYFDTAALA 121
SnoaL_2 pfam12680
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 13-111 6.34e-19

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 432716 [Multi-domain]  Cd Length: 100  Bit Score: 76.17  E-value: 6.34e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376   13 EYYAGLDAQPPAALATLYHPDATLSDPFGQHQGLFAIQRYFTHLLANVEQCRFTIDTPLCDGQRFAVTWTMHWSHPriAG 92
Cdd:pfam12680   3 RFYEALNAGDLDALAALFAPDAVFHDPGGPLRGRDAIRAFFAALFAAFPDLRFEIHDVIADGDRVAVRWTVTGTIP--PT 80

                          90
                  ....*....|....*....
gi 488976376   93 GETLALPGCSVVDIAGEQV 111
Cdd:pfam12680  81 GRGVRVRGVDVFRFRDGKI 99
ketosteroid_isomerase cd00781
ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond ...
 13-85 2.43e-06

ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.


Pssm-ID: 238404  Cd Length: 122  Bit Score: 43.95  E-value: 2.43e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 488976376  13 EYYAGLDAQPPAALATLYHPDATLSDPFGQ--HQGLFAIQRYFTHLLANVEqcRFTIDTPL----CDGQRFAVTWTMHW 85
Cdd:cd00781   11 RYVEAVNAGDPEGIVALFADDATVEDPVGSppRSGRAAIAAFYAQSLGGAK--RLELTGPVrashGGEAAFAFRVEFEW 87
 
Name Accession Description Interval E-value
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
7-124 7.46e-20

Ketosteroid isomerase-related protein [General function prediction only];


Pssm-ID: 442849  Cd Length: 126  Bit Score: 79.20  E-value: 7.46e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376   7 VIRRfveYYAGLDAQPPAALATLYHPDATLSDPF----GQHQGLFAIQRYFTHLLANVEQCRFTIDTPLCDGQRFAVTWT 82
Cdd:COG3631    5 LVRR---FYAAFNAGDVDALLALLAEDVVWEDPGgpgaGTYRGKEAVRAFFARLAAAFEDLRFEVRRVVADGDRVVVEGT 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 488976376  83 MHWSHPriAGGETLALPGCSVVDIAGEQVLHQRDYYDAGEMI 124
Cdd:COG3631   82 VRGTVL--ATGKPYENRYADVFTVRDGKIVRVREYFDTAALA 121
SnoaL_2 pfam12680
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 13-111 6.34e-19

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 432716 [Multi-domain]  Cd Length: 100  Bit Score: 76.17  E-value: 6.34e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376   13 EYYAGLDAQPPAALATLYHPDATLSDPFGQHQGLFAIQRYFTHLLANVEQCRFTIDTPLCDGQRFAVTWTMHWSHPriAG 92
Cdd:pfam12680   3 RFYEALNAGDLDALAALFAPDAVFHDPGGPLRGRDAIRAFFAALFAAFPDLRFEIHDVIADGDRVAVRWTVTGTIP--PT 80

                          90
                  ....*....|....*....
gi 488976376   93 GETLALPGCSVVDIAGEQV 111
Cdd:pfam12680  81 GRGVRVRGVDVFRFRDGKI 99
NTF2 pfam02136
Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like ...
 7-85 6.29e-09

Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like Delta-5-3-ketosteroid isomerase proteins.


Pssm-ID: 396625  Cd Length: 116  Bit Score: 50.43  E-value: 6.29e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376    7 VIRRFVE-YYAGLDAQPPAALATLYHPDATLSDPFG--QHQGLFAIQRYFTHLLANveQCRFTIDTplCDGQRFAVTWTM 83
Cdd:pfam02136   1 VGNAFVQqYYAALDAHDPEGLHALYADDASMLTPPGsdPVVGLEAINEFFDSLPFT--RIQHKITS--VDSQPASLKDGV 76


                  ..
gi 488976376   84 HW 85
Cdd:pfam02136  77 LV 78
ketosteroid_isomerase cd00781
ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond ...
 13-85 2.43e-06

ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.


Pssm-ID: 238404  Cd Length: 122  Bit Score: 43.95  E-value: 2.43e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 488976376  13 EYYAGLDAQPPAALATLYHPDATLSDPFGQ--HQGLFAIQRYFTHLLANVEqcRFTIDTPL----CDGQRFAVTWTMHW 85
Cdd:cd00781   11 RYVEAVNAGDPEGIVALFADDATVEDPVGSppRSGRAAIAAFYAQSLGGAK--RLELTGPVrashGGEAAFAFRVEFEW 87
COG5485 COG5485
Predicted ester cyclase [General function prediction only];
7-123 4.00e-05

Predicted ester cyclase [General function prediction only];


Pssm-ID: 444236 [Multi-domain]  Cd Length: 130  Bit Score: 40.61  E-value: 4.00e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376   7 VIRRFVEyyAGLDAQPPAALATLYHPDATLSDPF-GQHQGLFAIQRYFTHLLANVEQCRFTIDTPLCDGQRFAVTWTMHW 85
Cdd:COG5485    1 LVRRFFE--EAWNEGDLDALDELVAPDFVYHGPLpGEPRGPEGFRAFLRGLRAAFPDLRFEIEDLIAEGDRVAVRWTFTG 78

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 488976376  86 SH----PRIAG-GETLALPGCSVVDIAGEQVLHQRDYYDAGEM 123
Cdd:COG5485   79 THtgefLGIPPtGKRVEFRGIDIYRFEDGKIVEEWVVEDLLGL 121
SnoaL_4 pfam13577
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 8-101 1.83e-04

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 433323 [Multi-domain]  Cd Length: 125  Bit Score: 38.85  E-value: 1.83e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376    8 IRRFVEYYA-GLDAQPPAALATLYHPDATLS-DPFGQHQGLFAIQRYFTHLLANVEQCRFTIDTPL--CDGQRFAVTWTM 83
Cdd:pfam13577   9 IRDLVARYGrALDTGDWDELAALFTEDAVFDyGGLGVLEGRDAIVAGLRAALDGFLLTQHLLGNPVitVDGDTATGRAYL 88

                          90
                  ....*....|....*...
gi 488976376   84 HWSHPRIAGGETLALPGC 101
Cdd:pfam13577  89 LATHVGPGDGGPEVLRGG 106
NTF2_like cd00531
Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 ...
 8-85 3.55e-04

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.


Pssm-ID: 238296 [Multi-domain]  Cd Length: 124  Bit Score: 37.88  E-value: 3.55e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376   8 IRRFV-EYYAGLDAQPPAALATLYHPDATLSDPFG-----QHQGLFAIQRYFTHLLANVEQCRFTIDTPLC------DGQ 75
Cdd:cd00531    1 AEQFLyRYARLLDAGDREWLALLYADDAYFEPPGGdgliyPDDGREAIEDRVRRLPFGPSRTRHLVSNVDVqpgddgEGV 80

                         90
                 ....*....|
gi 488976376  76 RFAVTWTMHW 85
Cdd:cd00531   81 VVSVFGVLRT 90
YybH COG4319
Ketosteroid isomerase homolog YybH [General function prediction only];
7-100 1.65e-03

Ketosteroid isomerase homolog YybH [General function prediction only];


Pssm-ID: 443460 [Multi-domain]  Cd Length: 131  Bit Score: 36.27  E-value: 1.65e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376   7 VIRRFVEYYAGLDAqppAALATLYHPDATLSDPFGQ-HQGLFAIQRYFTHLLANVEQCRFTID--TPLCDGQRFAVTWTM 83
Cdd:COG4319   14 LLAAFAEAFNAGDA---DALAALYAEDAVFFDPGGPpVRGREAIRAAWAAAFAAGPRVTFEVEdvRVLVSGDVAVVTGRW 90

                         90
                 ....*....|....*..
gi 488976376  84 HWsHPRIAGGETLALPG 100
Cdd:COG4319   91 RL-TGTDPDGEPVELAG 106
SnoaL pfam07366
SnoaL-like polyketide cyclase; This family includes SnoaL a polyketide cyclase involved in ...
 7-119 3.30e-03

SnoaL-like polyketide cyclase; This family includes SnoaL a polyketide cyclase involved in nogalamycin biosynthesis. This family was formerly known as DUF1486. The proteins in this family adopt a distorted alpha-beta barrel fold. Structural data together with site-directed mutagenesis experiments have shown that SnoaL has a different mechanism to that of the classical aldolase for catalysing intramolecular aldol condensation.


Pssm-ID: 399975  Cd Length: 126  Bit Score: 35.35  E-value: 3.30e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488976376    7 VIRRFveYYAGLDAQPPAALATLYHPD-ATLSDPFGQHQGLFAIQRYFTHLLANVEQCRFTIDTPLCDGQRFAVTWTMHW 85
Cdd:pfam07366   2 LVLRF--YDEVWNTGDLDALDEFVAEDvVDHNPPPGVARGLDGFRAFAEMLFRAFPDLRFEVEDLVAEGDKVAARLTFTG 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 488976376   86 SHPriagGETLALP---------GCSVVDIAGEQVLHQRDYYD 119
Cdd:pfam07366  80 THT----GEFLGIPptgrkvrfrGIDLYRFEDGKIVEHWDVID 118
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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