MULTISPECIES: hypothetical protein [Pseudoalteromonas]
Protein Classification
site-specific integrase( domain architecture ID 332)
tyrosine based site-specific recombinase (integrase) is involved in cleavage of a single strand of a DNA duplex by nucleophilic attack of a conserved tyrosine to give a 3' phosphotyrosyl protein-DNA adduct
List of domain hits
| Name | Accession | Description | Interval | E-value | ||||
| DNA_BRE_C super family | cl00213 | DNA breaking-rejoining enzymes, C-terminal catalytic domain; The DNA breaking-rejoining enzyme ... |
10-169 | 1.77e-24 | ||||
DNA breaking-rejoining enzymes, C-terminal catalytic domain; The DNA breaking-rejoining enzyme superfamily includes type IB topoisomerases and tyrosine based site-specific recombinases (integrases) that share the same fold in their catalytic domain containing conserved active site residues. The best-studied members of this diverse superfamily include Human topoisomerase I, the bacteriophage lambda integrase, the bacteriophage P1 Cre recombinase, the yeast Flp recombinase, and the bacterial XerD/C recombinases. Their overall reaction mechanism is essentially identical and involves cleavage of a single strand of a DNA duplex by nucleophilic attack of a conserved tyrosine to give a 3' phosphotyrosyl protein-DNA adduct. In the second rejoining step, a terminal 5' hydroxyl attacks the covalent adduct to release the enzyme and generate duplex DNA. The enzymes differ in that topoisomerases cleave and then rejoin the same 5' and 3' termini, whereas a site-specific recombinase transfers a 5' hydroxyl generated by recombinase cleavage to a new 3' phosphate partner located in a different duplex region. Many DNA breaking-rejoining enzymes also have N-terminal domains, which show little sequence or structure similarity. The actual alignment was detected with superfamily member cd01184: Pssm-ID: 469662 [Multi-domain] Cd Length: 180 Bit Score: 95.45 E-value: 1.77e-24
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| Name | Accession | Description | Interval | E-value | ||||
| INT_C_like_1 | cd01184 | Uncharacterized site-specific tyrosine recombinase, C-terminal catalytic domain; Tyrosine ... |
10-169 | 1.77e-24 | ||||
Uncharacterized site-specific tyrosine recombinase, C-terminal catalytic domain; Tyrosine recombinase (integrase) belongs to a DNA breaking-rejoining enzyme superfamily. The catalytic domain containing six conserved active site residues. The recombination reaction involves cleavage of a single strand of a DNA duplex by nucleophilic attack of a conserved tyrosine to give a 3' phosphotyrosyl protein-DNA adduct. In the second rejoining step, a terminal 5' hydroxyl attacks the covalent adduct to release the enzyme and generate duplex DNA. Many DNA breaking-rejoining enzymes also have N-terminal domains, which show little sequence or structure similarity. Pssm-ID: 271184 [Multi-domain] Cd Length: 180 Bit Score: 95.45 E-value: 1.77e-24
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| Name | Accession | Description | Interval | E-value | ||||
| INT_C_like_1 | cd01184 | Uncharacterized site-specific tyrosine recombinase, C-terminal catalytic domain; Tyrosine ... |
10-169 | 1.77e-24 | ||||
Uncharacterized site-specific tyrosine recombinase, C-terminal catalytic domain; Tyrosine recombinase (integrase) belongs to a DNA breaking-rejoining enzyme superfamily. The catalytic domain containing six conserved active site residues. The recombination reaction involves cleavage of a single strand of a DNA duplex by nucleophilic attack of a conserved tyrosine to give a 3' phosphotyrosyl protein-DNA adduct. In the second rejoining step, a terminal 5' hydroxyl attacks the covalent adduct to release the enzyme and generate duplex DNA. Many DNA breaking-rejoining enzymes also have N-terminal domains, which show little sequence or structure similarity. Pssm-ID: 271184 [Multi-domain] Cd Length: 180 Bit Score: 95.45 E-value: 1.77e-24
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