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MULTISPECIES: glycyl radical protein [Citrobacter]
Protein Classification
glycyl radical protein ( domain architecture ID 11493154 )
glycyl radical protein similar to formate acetyltransferase, benzylsuccinate synthase, 4-hydroxyphenylacetate decarboxylase, and CutC choline trimethylamine-lyase
List of domain hits
Name
Accession
Description
Interval
E-value
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1372.29
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 8 TLS DRI KAH K T AL IHI V k PPVCT ERA QH YTE MYQ Q HLDK P IPV RRA L ALAH H L AERT I W I KHD ELI I GN QASEV RAA P IF 87
Cdd:TIGR01774 1 MCM DRI EKL K K AL SKP V - ARLSV ERA RL YTE SMK Q TEGE P MII RRA K ALAH V L ENIP I Q I LDS ELI V GN MLPNP RAA I IF 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 88 PE YTVS WI E KE I D D L AD RP GAG FAVSEE N KRVL H - E VC P W W RGQ T VQ D RCY G MF TDE Q K G L LE T G I IKAE G n MTS G DA H L 166
Cdd:TIGR01774 80 PE GVGL WI I KE L D S L PN RP TNR FAVSEE D KRVL R e E IA P Y W QRK T IE D FAF G LM TDE M K I L YT T S I FVLT G - EIA G IS H V 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 167 AVN F P L LL EK GL DG L RHKVDE R RSRINLTCLDDLHGDQ F LK A ID IVLEAV TL HI E R F A A LA RK MA ST ET RT SRR D ELL TM 246
Cdd:TIGR01774 159 AVN Y P Y LL RR GL RW L LEESER R IRALEESGVYEGEKYS F YQ A AK IVLEAV IN HI L R Y A K LA EE MA AS ET GE SRR E ELL KI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 247 AE N C DVI A H E P P K TFWQA L QL CYFI Q L ILQ I ESN GH S V S F GR M DQYLYPFY RR D VELNQ s L DRE H A I E L L H S C W L K LL E V 326
Cdd:TIGR01774 239 AE I C RKV A A E K P Q TFWQA V QL VWLV Q S ILQ Q ESN EQ S I S M GR I DQYLYPFY KK D IGEGR - I DRE L A F E I L A S L W I K TN E I 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 327 NKI RS G S HSKAS AG S P LY Q N VTIGG QN lvs GQAM DAVN P LSY AI LE SCG RLR ST QPNL S VR YH AG MSND FL DACVQV IR C 406
Cdd:TIGR01774 318 VPA RS S S LEQYF AG Q P TN Q A VTIGG CD --- IYGN DAVN E LSY LM LE VTD RLR LR QPNL H VR IN AG SPES FL KRLAEA IR S 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 407 G F G M PA FN NDE I V I P E F IKL G IEPQ DA Y DY AAI GC I E T A VG G KW g Y RCTGMSF IN F A R VM LA AL EG G R D ATS G KV F LPQE 486
Cdd:TIGR01774 395 G C G N PA LF NDE A V V P A F KNA G VDDR DA L DY TTD GC V E I A PF G NS - Y TSSDAAL IN V A K VM EY AL NE G V D LQF G YE F GAKT 473
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 487 KAL sagnf NNFDEVMDAWDT QI RY Y TRKSI E IEY V V D TMLE E NVHDI L C S AL V D DC I E RAKS I KQ GGA K Y DWVSGLQ VGI 566
Cdd:TIGR01774 474 EKP ----- KFLEDLLEKLRE QI SH Y VKLVV E GSN V L D YANA E VKPTP L L S LC V E DC F E KGVD I SR GGA R Y NFTGIQA VGI 548
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 567 ANLG N SL A A V K KL VFEQGA IGQQQ L AA AL ADD F D GLTHE QL R Q RLIN GA PKYGNDDD S VD TLL A RAYQT Y ID E LKQ Y H NP 646
Cdd:TIGR01774 549 ANLG D SL V A I K GA VFEQGA YSMDD L VE AL RKN F V GLTHE EK R H RLIN QS PKYGNDDD A VD KYT A MVLEW Y CE E VNR Y R NP 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 647 R ygrgpv GG NYY AG TSSI SANVPFG AA T M ATPDGRKA HT PLA E G A SP AS G T D HL GPTAVI G SV G KL PTGSILG G V LLN Q K 726
Cdd:TIGR01774 629 R ------ GG KFA AG CYPM SANVPFG FF T S ATPDGRKA GE PLA P G V SP ST G M D RE GPTAVI N SV S KL SYENLPN G A LLN I K 702
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 727 L N PATLE N E S DKQKL MIL LR TF F E E H K GW HIQ Y NIV SRE TL LE A KKH P DQ YR D LVVRVAGYSAFF TA LS PDA QDDII A RT 806
Cdd:TIGR01774 703 L S PATLE E E G DKQKL IEA LR KS F M E L K VM HIQ F NIV KED TL RK A QQE P EK YR W LVVRVAGYSAFF VE LS RPV QDDII R RT 782
....
gi 489125480 807 E H M L 810
Cdd:TIGR01774 783 S H R L 786
Name
Accession
Description
Interval
E-value
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1372.29
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 8 TLS DRI KAH K T AL IHI V k PPVCT ERA QH YTE MYQ Q HLDK P IPV RRA L ALAH H L AERT I W I KHD ELI I GN QASEV RAA P IF 87
Cdd:TIGR01774 1 MCM DRI EKL K K AL SKP V - ARLSV ERA RL YTE SMK Q TEGE P MII RRA K ALAH V L ENIP I Q I LDS ELI V GN MLPNP RAA I IF 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 88 PE YTVS WI E KE I D D L AD RP GAG FAVSEE N KRVL H - E VC P W W RGQ T VQ D RCY G MF TDE Q K G L LE T G I IKAE G n MTS G DA H L 166
Cdd:TIGR01774 80 PE GVGL WI I KE L D S L PN RP TNR FAVSEE D KRVL R e E IA P Y W QRK T IE D FAF G LM TDE M K I L YT T S I FVLT G - EIA G IS H V 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 167 AVN F P L LL EK GL DG L RHKVDE R RSRINLTCLDDLHGDQ F LK A ID IVLEAV TL HI E R F A A LA RK MA ST ET RT SRR D ELL TM 246
Cdd:TIGR01774 159 AVN Y P Y LL RR GL RW L LEESER R IRALEESGVYEGEKYS F YQ A AK IVLEAV IN HI L R Y A K LA EE MA AS ET GE SRR E ELL KI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 247 AE N C DVI A H E P P K TFWQA L QL CYFI Q L ILQ I ESN GH S V S F GR M DQYLYPFY RR D VELNQ s L DRE H A I E L L H S C W L K LL E V 326
Cdd:TIGR01774 239 AE I C RKV A A E K P Q TFWQA V QL VWLV Q S ILQ Q ESN EQ S I S M GR I DQYLYPFY KK D IGEGR - I DRE L A F E I L A S L W I K TN E I 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 327 NKI RS G S HSKAS AG S P LY Q N VTIGG QN lvs GQAM DAVN P LSY AI LE SCG RLR ST QPNL S VR YH AG MSND FL DACVQV IR C 406
Cdd:TIGR01774 318 VPA RS S S LEQYF AG Q P TN Q A VTIGG CD --- IYGN DAVN E LSY LM LE VTD RLR LR QPNL H VR IN AG SPES FL KRLAEA IR S 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 407 G F G M PA FN NDE I V I P E F IKL G IEPQ DA Y DY AAI GC I E T A VG G KW g Y RCTGMSF IN F A R VM LA AL EG G R D ATS G KV F LPQE 486
Cdd:TIGR01774 395 G C G N PA LF NDE A V V P A F KNA G VDDR DA L DY TTD GC V E I A PF G NS - Y TSSDAAL IN V A K VM EY AL NE G V D LQF G YE F GAKT 473
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 487 KAL sagnf NNFDEVMDAWDT QI RY Y TRKSI E IEY V V D TMLE E NVHDI L C S AL V D DC I E RAKS I KQ GGA K Y DWVSGLQ VGI 566
Cdd:TIGR01774 474 EKP ----- KFLEDLLEKLRE QI SH Y VKLVV E GSN V L D YANA E VKPTP L L S LC V E DC F E KGVD I SR GGA R Y NFTGIQA VGI 548
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 567 ANLG N SL A A V K KL VFEQGA IGQQQ L AA AL ADD F D GLTHE QL R Q RLIN GA PKYGNDDD S VD TLL A RAYQT Y ID E LKQ Y H NP 646
Cdd:TIGR01774 549 ANLG D SL V A I K GA VFEQGA YSMDD L VE AL RKN F V GLTHE EK R H RLIN QS PKYGNDDD A VD KYT A MVLEW Y CE E VNR Y R NP 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 647 R ygrgpv GG NYY AG TSSI SANVPFG AA T M ATPDGRKA HT PLA E G A SP AS G T D HL GPTAVI G SV G KL PTGSILG G V LLN Q K 726
Cdd:TIGR01774 629 R ------ GG KFA AG CYPM SANVPFG FF T S ATPDGRKA GE PLA P G V SP ST G M D RE GPTAVI N SV S KL SYENLPN G A LLN I K 702
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 727 L N PATLE N E S DKQKL MIL LR TF F E E H K GW HIQ Y NIV SRE TL LE A KKH P DQ YR D LVVRVAGYSAFF TA LS PDA QDDII A RT 806
Cdd:TIGR01774 703 L S PATLE E E G DKQKL IEA LR KS F M E L K VM HIQ F NIV KED TL RK A QQE P EK YR W LVVRVAGYSAFF VE LS RPV QDDII R RT 782
....
gi 489125480 807 E H M L 810
Cdd:TIGR01774 783 S H R L 786
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
10-807
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1162.43
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 10 SD RIK AH K TALIHI v KP PVCT ERA QH YTE M Y QQHLD KP IPV RRA L AL A H H L AER TI W I KH DELI I GN QASEV RA A P I FPE 89
Cdd:cd01677 1 TE RIK RL K AKILTA - KP SISI ERA RL YTE S Y KETEG KP VII RRA K AL K H I L ENA TI Y I QD DELI V GN RGGKP RA V P V FPE 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 90 YT V S W I E K E I DDL AD RPG AG F AV SE EN K RV L H E VC P W W R G Q T VQ DRC YGM F TD E QKGLLET G IIKAEGNMT SG DA H L AV N 169
Cdd:cd01677 80 LS V H W V E D E L DDL PK RPG DP F VI SE DK K EY L E E IF P Y W K G K T LR DRC FKY F PE E TLIAMAA G VFTEFMYFF SG PG H V AV D 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 170 F P LL LEKGLDGL RHKVD E RRSRIN LT CLD D LHGDQ F LK A ID IV L EAV TLHIE R F A A LA RKM A ST ET RTS R RD ELL TM AE N 249
Cdd:cd01677 160 Y P KV LEKGLDGL IEEAK E AIEALD LT GPE D IDKIY F YQ A MI IV C EAV ITYAK R Y A E LA KEL A AK ET DPK R KA ELL EI AE I 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 250 C DVIAHE PP K TFW Q ALQ LCY FI Q LILQIESNGHS V S F GR M DQYLYPFY RR D V E LN q S L D RE H AIELL HSC W L K LL E V NK I 329
Cdd:cd01677 240 C RRVPAH PP R TFW E ALQ SFW FI H LILQIESNGHS I S P GR F DQYLYPFY KQ D I E EG - R L T RE G AIELL ECL W I K IN E I NK V 318
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 330 RSG SHS K AS AG SPLY QN V TIGGQ NLVSG qam DA V N P LSY A ILE SCG R L R ST QP N L S VRYHA GMSND FL DACVQ VIR C G F G 409
Cdd:cd01677 319 RSG ASA K YF AG YNTF QN L TIGGQ TEDGS --- DA T N E LSY L ILE ATR R V R LP QP S L T VRYHA KSPDK FL KKAAE VIR L G L G 395
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 410 M PAF N NDE I VIP EFIKL G IEPQ DA Y DY AA IGC I ET AVG G k WG YR C TG MSF IN F A R V MLAA L EG G R D AT SGK VFL P Q ek AL 489
Cdd:cd01677 396 Y PAF F NDE V VIP ALLRK G VSLE DA R DY GL IGC V ET GAP G - RK YR W TG TGY IN L A K V LEIT L NN G K D PR SGK QVG P E -- TG 472
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 490 S A GN F NN F D E VMD A WDT Q I R YYTRK S IEIEYVV D TMLE E NVHDILC SALVDDCIE RA K S I KQ GGA K Y DWVSGLQ VGIA N L 569
Cdd:cd01677 473 D A TD F KT F E E LWE A FKK Q L R HFIKL S VRANNIS D IAHA E VAPAPFL SALVDDCIE KG K D I NA GGA R Y NFGGIQG VGIA T L 552
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 570 G N SLAA V KKLVFE QGAIGQQQ L AA AL ADD F DG lt HEQL R Q RL I N G APKYGNDDD SV D TLLA R A Y QT Y ID E LKQ Y H NPR yg 649
Cdd:cd01677 553 G D SLAA I KKLVFE EKKLTMEE L LE AL KAN F AE -- GYEE R R RL L N N APKYGNDDD YA D NIAR R V Y EW Y CK E VEK Y Q NPR -- 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 650 rgpv GG NY Y A GT S S I SANVPFG AA T M ATPDGR K A H TPL AE G A SP AS GTD HL GPTAVI G SV G KL PTGS I L GG V LLNQK LN P 729
Cdd:cd01677 629 ---- GG KF Y P GT Y S V SANVPFG SV T G ATPDGR L A G TPL SD G V SP SQ GTD KK GPTAVI K SV S KL DHFN I S GG T LLNQK FS P 704
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489125480 730 A TLE N E SDKQ KL MI L L RT F F EE h K G W HIQ Y N I VS R ETL LE A K KHP DQ YRDL V VRVAGYSA F F TA LS PDA QD D IIARTE 807
Cdd:cd01677 705 S TLE G E EGLK KL AA L I RT Y F DL - G G H HIQ F N V VS A ETL RD A Q KHP EK YRDL I VRVAGYSA Y F VE LS KEV QD E IIARTE 781
PflD
COG1882 Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
6-808
0e+00
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain]
Cd Length: 789
Bit Score: 1063.23
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 6 L DTLSD R I K AHKTA L IH i V KP PVCT ERA QHY TE M Y QQHLDK P IPV RRA L A LA H H L AERT I W IK H DELI I G N Q ASEV R AA P 85
Cdd:COG1882 4 L AGPTE R T K RLREK L LE - A KP LIDI ERA RLF TE S Y KETEGL P VII RRA K A FS H I L EHKP I Y IK D DELI V G L Q TDKP R KR P 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 86 IFPE YTVS W I E K E I D D L AD RP GA GF AV S E E N K RVLH E VC P W W R G Q T VQ D RCYGMFTD E QKGLLET GII K a EGNMTS G DA H 165
Cdd:COG1882 83 IFPE GGIR W V E D E L D A L PT RP QD GF EI S P E D K EIFR E IA P Y W K G K T HN D GVFDAYPE E IRKARKA GII T - GLPDAY G RG H 161
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 166 LAVNFPLL L EK GLDGL RHKVD E RRSRIN LT CLD D LHGDQ F L KA ID IV L EAV TLHI ER F A A LAR KM A ST ET RTS R RD ELL T 245
Cdd:COG1882 162 IIGDYRRV L LY GLDGL IEEAK E KLAELD LT DPE D IEKID F Y KA MI IV C EAV IRLA ER Y A E LAR EL A EK ET DPK R KA ELL E 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 246 M AE N C DVIAHE P PK TFW Q A L Q LCY F IQ L ILQI E S NG HSV S F GR M DQYLYP F Y R RD V E l NQS L DR E H A I ELL HSC W L KL L E 325
Cdd:COG1882 242 I AE I C GFVPAN P AR TFW E A V Q WVW F VY L AAIK E Q NG AAM S L GR F DQYLYP Y Y E RD L E - EGR L TE E E A Q ELL DCF W I KL R E 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 326 V NKI R SGSHSKAS AG S P LYQNV TIGG QN l VS G Q am DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ VI R 405
Cdd:COG1882 321 V RFL R TPEYAELF AG Y P TWVTL TIGG MT - PD G R -- DAVN E LSY L ILE TLRN L PLPE PNL T VR WSEKLPEG FL KKAAE VI S 397
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 406 C G F G M P AFN ND EIV IP EFIKL G IEPQ DA Y DY AAI GC I E TA V G GK W g YRCT G MSF IN F A RVMLA AL EG G R D ATS GK VFL P Q 485
Cdd:COG1882 398 I G T G S P QYE ND DLM IP MLLNK G VTLE DA R DY GIA GC V E PM V P GK Q - MQFF G AGR IN L A KALEY AL NN G V D EKT GK QVG P E 476
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 486 EKALS ag N F NNF DEVM D A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV G 565
Cdd:COG1882 477 TGDPT -- D F LTY DEVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DLNE GGA R Y NFGAIGIA G 554
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 566 IANLGN SL A A V KKLVF EQGAIGQQQ L AA ALA DD F D G lt H E Q LRQ R L I N g APKYGNDDD S VD TLLARAYQ T YI DE LKQ Y HN 645
Cdd:COG1882 555 LSVVAD SL S A I KKLVF DKKKVTMDE L LE ALA AN F E G -- Y E E LRQ L L L N - APKYGNDDD Y VD EIAVELVE T FM DE IRK Y KT 631
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 646 P R ygrgpv GG N Y YAGTSS I SA NVP F G AA T M ATPDGRKA HT PLA E GASP AS G T D HL GPTAV IG SV G KLP TGSILG G V LLNQ 725
Cdd:COG1882 632 Y R ------ GG T Y TLSILT I TS NVP Y G KK T G ATPDGRKA GE PLA D GASP MH G R D KN GPTAV LK SV A KLP YEKATD G I LLNQ 705
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 726 K LN P AT L EN E SDKQK L MI LLRT F F e EHK G W HIQ Y N I V S RETLL E A K KHP DQ Y R DL V VRVAGYSA F F TA LS PDA QDDIIAR 805
Cdd:COG1882 706 K FS P SA L GG E EGIEN L VS LLRT Y F - DLG G H HIQ F N V V D RETLL D A Q KHP EK Y P DL T VRVAGYSA Y F VE LS KEQ QDDIIAR 784
...
gi 489125480 806 TEH 808
Cdd:COG1882 785 TEH 787
PFL-like
pfam02901 Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
11-671
0e+00
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048
Cd Length: 647
Bit Score: 735.59
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 11 D RI K AHK T al IHIVK P PVCT ERA QHY TE M Y QQHLDK - P IPV RRA L AL AHH L AERTIW I KH DELI I G N Q ASEV R AAP I F PE 89
Cdd:pfam02901 6 D VL K ENY T -- LYTGD P SLSW ERA RLL TE S Y KETEGV l P VDI RRA K AL KKI L SHLPGY I RD DELI V G L Q TDKP R KRA I Y PE 83
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 90 YTVS W I E K E I D D L AD RP GA GF AV SEE N K RVLH E VC P W W R G Q T VQDRCYGMF T D E Q K GLL E T GI IK a EGNMTS G DA H LAVN 169
Cdd:pfam02901 84 GGIR W V E D E L D Y L NT RP QD GF EI SEE D K KIFR E IF P Y W K G K T HNEGVFDAY T P E M K AAR E S GI FT - GLPDAY G RG H IIGD 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 170 FPLL L EK GLDGL RHKVD E RRSRINLTC l D D LHGDQ F L KA ID I VLE AV TLHI ER F A A LA RKM A ST ET RTS R RD ELL TM AE N 249
Cdd:pfam02901 163 YRRV L LY GLDGL IEEKE E KLAKLDTDP - E D IEKIE F Y KA MI I SCD AV IEYA ER Y A R LA EEL A EQ ET DPK R KA ELL EI AE I 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 250 C DVIAHE P PK TF WQ A L Q LCY F IQ L ILQI E S NG HSV S F GR M DQYLYP F Y R RD V E l NQS L DR E H A I EL LHSC W L KL L EV NKI 329
Cdd:pfam02901 242 C GRVPAR P AE TF QE A I Q WFW F VY L AAVK E Q NG AAM S L GR L DQYLYP Y Y E RD L E - EGR L TE E E A Q EL IDCF W I KL R EV RFL 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 330 R SGSHS K AS AG SPLY QN V TIGGQ NL v S G Q am DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ V I R C G F G 409
Cdd:pfam02901 321 R TPEYN K LF AG YDPF QN L TIGGQ GR - D G R -- DAVN K LSY L ILE ALDN L PLPE PNL T VR WSKKLPEE FL KKAAE V S R K G T G 397
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 410 M P AFN ND EIV IP EFIKL G IEPQ DA Y DY AAI GC I E TAVG GK - WGYRC tgm SF IN F A RVMLA AL E GGRD ATS GK VFL P QEKA 488
Cdd:pfam02901 398 S P QYE ND DVM IP ALLNR G VSLE DA R DY GIA GC V E PMKP GK e MQFFG --- AR IN L A KALEY AL N GGRD ELT GK QVG P KTGP 474
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 489 LS ag N F NN F D EVM D A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV G I AN 568
Cdd:pfam02901 475 VT -- E F LS F E EVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DVGI GGA R Y NFSGPQGA G L AN 552
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 569 LGN SL A A V KKLVF EQGAIGQQQ L AA ALA D DF D G lt H E Q LRQ R L I N G APKYGNDDD S VD TLLARAYQ T YI DE LKQ Y H N P R y 648
Cdd:pfam02901 553 VAD SL S A I KKLVF DDKVYTLRE L ED ALA A DF E G -- E E E LRQ D L L N D APKYGNDDD R VD DIAVEVVE T FM DE VRK Y K N Y R - 629
650 660
....*....|....*....|...
gi 489125480 649 grgpv GG NYYAGTSS I SA NVP F G 671
Cdd:pfam02901 630 ----- GG KFTPSLLT I TS NVP Y G 647
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
9-810
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 585.25
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 9 LSD RI KAH KTAL IHIVKP p VCT ERA QH YT EMYQ Q HLDK P IPV RRA L A L A HH L AERT I W I KHD ELI I GN QASEV RA APIF P 88
Cdd:PRK09983 1 MTN RI SRL KTAL FANTRE - ISL ERA LL YT ASHR Q TEGE P VIL RRA K A T A YI L EHVE I S I RDE ELI A GN RTVKP RA GIMS P 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 89 E YTVS W IE KE I D DLAD RP GAG FA V SEE N KR VLH E VC - P W W RGQTVQ D RCY G MF TDE Q K GLLE T G I IKA eg N M T - S G DA H L 166
Cdd:PRK09983 80 E MDPY W LL KE L D QFPT RP QDR FA I SEE D KR IYR E EL f P Y W EKRSMK D FIN G QM TDE V K AATS T Q I FSI -- N Q T d K G QG H I 157
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 167 AVNF P L LL EK GL DG L R hkvderr SRINLT C LDDLHGD q F LK A IDIV LEA VTL HI E R F A A LA RK MA STE T RTS RR D ELLT M 246
Cdd:PRK09983 158 IIDY P R LL NH GL GE L V ------- AQMQQH C QQQPENH - F YQ A ALLL LEA SQK HI L R Y A E LA ET MA ANC T DAQ RR E ELLT I 229
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 247 AE NCDVI A HEP P K TFWQA L QL CYFIQL ILQ I ESN GH S V S F GR M DQY LY PFY RRD ve L N Q SL D REHAI ELL H S C W L K LLEV 326
Cdd:PRK09983 230 AE ISRHN A QHK P Q TFWQA C QL FWYMNI ILQ Y ESN AS S L S L GR F DQY ML PFY QAS -- L T Q GE D PAFLK ELL E S L W V K CNDI 307
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 327 NKI RS G S HSKAS AG S P LYQNVTI GG qnl VSGQAMD AVN P LS YAI L ESCGRLRST QPNL S VR YH A GMSND FL DACVQV IR C 406
Cdd:PRK09983 308 VLL RS T S SARYF AG F P TGYTALL GG --- LTENGRS AVN V LS FLC L DAYQSVQLP QPNL G VR TN A LIDTP FL MKTAET IR L 384
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 407 G F G M P AFN NDE I V I P E F IKL G IEPQ DA Y DY AAI GC I E TAVG G K w G Y RCTGMSFI N FAR VM LAA L --- EG GRDA T sgkvfl 483
Cdd:PRK09983 385 G T G I P QIF NDE V V V P A F LNR G VSLE DA R DY SVV GC V E LSIP G R - T Y GLHDIAMF N LLK VM EIC L hen EG NAAL T ------ 457
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 484 pqekalsagnfnn FDEVMDAWDTQ I RY Y TRKSI E IEYVV D TMLEENVHDI L C S ALVD DC I E RAKS I KQ GGA K Y DW v SG L Q 563
Cdd:PRK09983 458 ------------- YEGLLEQIRAK I SH Y ITLMV E GSNIC D IGHRDWAPVP L L S SFIS DC L E KGRD I TD GGA R Y NF - SG V Q 523
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 564 - V GIANL GN SL A A V K KL VF E Q GAIGQQQ L AAA L ADD F DGLTH E QL R Q RLIN GAP KYGND D D S VD TLL A RAYQT Y ID E LKQ 642
Cdd:PRK09983 524 g I GIANL SD SL H A L K GM VF D Q QRLSFDE L LSV L KAN F ATPEG E KV R A RLIN RFE KYGND I D E VD NIS A ELLRH Y CK E VEK 603
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 643 Y H NPR ygrgpv GG NYYA G TSSI SA N VP F G AATM ATPDGR K A HTP LA E G A - SP AS G T D HL GPTAV IG SV G KL PTGSILG G V 721
Cdd:PRK09983 604 Y Q NPR ------ GG YFTP G SYTV SA H VP L G SVVG ATPDGR F A GEQ LA D G G l SP ML G Q D AQ GPTAV LK SV S KL DNTLLSN G T 677
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 722 LLN Q K LN PATLE N E SDKQ KL MIL LR T F F e EH K GW HIQ Y N I V SRE TL L EA KKH P DQ Y RD LVVRVAGYSAFF TA LS PDA QDD 801
Cdd:PRK09983 678 LLN V K FT PATLE G E AGLR KL ADF LR A F T - QL K LQ HIQ F N V V NAD TL R EA QQR P QD Y AG LVVRVAGYSAFF VE LS KEI QDD 756
....*....
gi 489125480 802 II A RT E H M L 810
Cdd:PRK09983 757 II R RT A H Q L 765
Name
Accession
Description
Interval
E-value
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
8-810
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 1372.29
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 8 TLS DRI KAH K T AL IHI V k PPVCT ERA QH YTE MYQ Q HLDK P IPV RRA L ALAH H L AERT I W I KHD ELI I GN QASEV RAA P IF 87
Cdd:TIGR01774 1 MCM DRI EKL K K AL SKP V - ARLSV ERA RL YTE SMK Q TEGE P MII RRA K ALAH V L ENIP I Q I LDS ELI V GN MLPNP RAA I IF 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 88 PE YTVS WI E KE I D D L AD RP GAG FAVSEE N KRVL H - E VC P W W RGQ T VQ D RCY G MF TDE Q K G L LE T G I IKAE G n MTS G DA H L 166
Cdd:TIGR01774 80 PE GVGL WI I KE L D S L PN RP TNR FAVSEE D KRVL R e E IA P Y W QRK T IE D FAF G LM TDE M K I L YT T S I FVLT G - EIA G IS H V 158
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 167 AVN F P L LL EK GL DG L RHKVDE R RSRINLTCLDDLHGDQ F LK A ID IVLEAV TL HI E R F A A LA RK MA ST ET RT SRR D ELL TM 246
Cdd:TIGR01774 159 AVN Y P Y LL RR GL RW L LEESER R IRALEESGVYEGEKYS F YQ A AK IVLEAV IN HI L R Y A K LA EE MA AS ET GE SRR E ELL KI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 247 AE N C DVI A H E P P K TFWQA L QL CYFI Q L ILQ I ESN GH S V S F GR M DQYLYPFY RR D VELNQ s L DRE H A I E L L H S C W L K LL E V 326
Cdd:TIGR01774 239 AE I C RKV A A E K P Q TFWQA V QL VWLV Q S ILQ Q ESN EQ S I S M GR I DQYLYPFY KK D IGEGR - I DRE L A F E I L A S L W I K TN E I 317
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 327 NKI RS G S HSKAS AG S P LY Q N VTIGG QN lvs GQAM DAVN P LSY AI LE SCG RLR ST QPNL S VR YH AG MSND FL DACVQV IR C 406
Cdd:TIGR01774 318 VPA RS S S LEQYF AG Q P TN Q A VTIGG CD --- IYGN DAVN E LSY LM LE VTD RLR LR QPNL H VR IN AG SPES FL KRLAEA IR S 394
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 407 G F G M PA FN NDE I V I P E F IKL G IEPQ DA Y DY AAI GC I E T A VG G KW g Y RCTGMSF IN F A R VM LA AL EG G R D ATS G KV F LPQE 486
Cdd:TIGR01774 395 G C G N PA LF NDE A V V P A F KNA G VDDR DA L DY TTD GC V E I A PF G NS - Y TSSDAAL IN V A K VM EY AL NE G V D LQF G YE F GAKT 473
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 487 KAL sagnf NNFDEVMDAWDT QI RY Y TRKSI E IEY V V D TMLE E NVHDI L C S AL V D DC I E RAKS I KQ GGA K Y DWVSGLQ VGI 566
Cdd:TIGR01774 474 EKP ----- KFLEDLLEKLRE QI SH Y VKLVV E GSN V L D YANA E VKPTP L L S LC V E DC F E KGVD I SR GGA R Y NFTGIQA VGI 548
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 567 ANLG N SL A A V K KL VFEQGA IGQQQ L AA AL ADD F D GLTHE QL R Q RLIN GA PKYGNDDD S VD TLL A RAYQT Y ID E LKQ Y H NP 646
Cdd:TIGR01774 549 ANLG D SL V A I K GA VFEQGA YSMDD L VE AL RKN F V GLTHE EK R H RLIN QS PKYGNDDD A VD KYT A MVLEW Y CE E VNR Y R NP 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 647 R ygrgpv GG NYY AG TSSI SANVPFG AA T M ATPDGRKA HT PLA E G A SP AS G T D HL GPTAVI G SV G KL PTGSILG G V LLN Q K 726
Cdd:TIGR01774 629 R ------ GG KFA AG CYPM SANVPFG FF T S ATPDGRKA GE PLA P G V SP ST G M D RE GPTAVI N SV S KL SYENLPN G A LLN I K 702
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 727 L N PATLE N E S DKQKL MIL LR TF F E E H K GW HIQ Y NIV SRE TL LE A KKH P DQ YR D LVVRVAGYSAFF TA LS PDA QDDII A RT 806
Cdd:TIGR01774 703 L S PATLE E E G DKQKL IEA LR KS F M E L K VM HIQ F NIV KED TL RK A QQE P EK YR W LVVRVAGYSAFF VE LS RPV QDDII R RT 782
....
gi 489125480 807 E H M L 810
Cdd:TIGR01774 783 S H R L 786
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
10-807
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1162.43
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 10 SD RIK AH K TALIHI v KP PVCT ERA QH YTE M Y QQHLD KP IPV RRA L AL A H H L AER TI W I KH DELI I GN QASEV RA A P I FPE 89
Cdd:cd01677 1 TE RIK RL K AKILTA - KP SISI ERA RL YTE S Y KETEG KP VII RRA K AL K H I L ENA TI Y I QD DELI V GN RGGKP RA V P V FPE 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 90 YT V S W I E K E I DDL AD RPG AG F AV SE EN K RV L H E VC P W W R G Q T VQ DRC YGM F TD E QKGLLET G IIKAEGNMT SG DA H L AV N 169
Cdd:cd01677 80 LS V H W V E D E L DDL PK RPG DP F VI SE DK K EY L E E IF P Y W K G K T LR DRC FKY F PE E TLIAMAA G VFTEFMYFF SG PG H V AV D 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 170 F P LL LEKGLDGL RHKVD E RRSRIN LT CLD D LHGDQ F LK A ID IV L EAV TLHIE R F A A LA RKM A ST ET RTS R RD ELL TM AE N 249
Cdd:cd01677 160 Y P KV LEKGLDGL IEEAK E AIEALD LT GPE D IDKIY F YQ A MI IV C EAV ITYAK R Y A E LA KEL A AK ET DPK R KA ELL EI AE I 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 250 C DVIAHE PP K TFW Q ALQ LCY FI Q LILQIESNGHS V S F GR M DQYLYPFY RR D V E LN q S L D RE H AIELL HSC W L K LL E V NK I 329
Cdd:cd01677 240 C RRVPAH PP R TFW E ALQ SFW FI H LILQIESNGHS I S P GR F DQYLYPFY KQ D I E EG - R L T RE G AIELL ECL W I K IN E I NK V 318
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 330 RSG SHS K AS AG SPLY QN V TIGGQ NLVSG qam DA V N P LSY A ILE SCG R L R ST QP N L S VRYHA GMSND FL DACVQ VIR C G F G 409
Cdd:cd01677 319 RSG ASA K YF AG YNTF QN L TIGGQ TEDGS --- DA T N E LSY L ILE ATR R V R LP QP S L T VRYHA KSPDK FL KKAAE VIR L G L G 395
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 410 M PAF N NDE I VIP EFIKL G IEPQ DA Y DY AA IGC I ET AVG G k WG YR C TG MSF IN F A R V MLAA L EG G R D AT SGK VFL P Q ek AL 489
Cdd:cd01677 396 Y PAF F NDE V VIP ALLRK G VSLE DA R DY GL IGC V ET GAP G - RK YR W TG TGY IN L A K V LEIT L NN G K D PR SGK QVG P E -- TG 472
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 490 S A GN F NN F D E VMD A WDT Q I R YYTRK S IEIEYVV D TMLE E NVHDILC SALVDDCIE RA K S I KQ GGA K Y DWVSGLQ VGIA N L 569
Cdd:cd01677 473 D A TD F KT F E E LWE A FKK Q L R HFIKL S VRANNIS D IAHA E VAPAPFL SALVDDCIE KG K D I NA GGA R Y NFGGIQG VGIA T L 552
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 570 G N SLAA V KKLVFE QGAIGQQQ L AA AL ADD F DG lt HEQL R Q RL I N G APKYGNDDD SV D TLLA R A Y QT Y ID E LKQ Y H NPR yg 649
Cdd:cd01677 553 G D SLAA I KKLVFE EKKLTMEE L LE AL KAN F AE -- GYEE R R RL L N N APKYGNDDD YA D NIAR R V Y EW Y CK E VEK Y Q NPR -- 628
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 650 rgpv GG NY Y A GT S S I SANVPFG AA T M ATPDGR K A H TPL AE G A SP AS GTD HL GPTAVI G SV G KL PTGS I L GG V LLNQK LN P 729
Cdd:cd01677 629 ---- GG KF Y P GT Y S V SANVPFG SV T G ATPDGR L A G TPL SD G V SP SQ GTD KK GPTAVI K SV S KL DHFN I S GG T LLNQK FS P 704
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489125480 730 A TLE N E SDKQ KL MI L L RT F F EE h K G W HIQ Y N I VS R ETL LE A K KHP DQ YRDL V VRVAGYSA F F TA LS PDA QD D IIARTE 807
Cdd:cd01677 705 S TLE G E EGLK KL AA L I RT Y F DL - G G H HIQ F N V VS A ETL RD A Q KHP EK YRDL I VRVAGYSA Y F VE LS KEV QD E IIARTE 781
PflD
COG1882 Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
6-808
0e+00
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain]
Cd Length: 789
Bit Score: 1063.23
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 6 L DTLSD R I K AHKTA L IH i V KP PVCT ERA QHY TE M Y QQHLDK P IPV RRA L A LA H H L AERT I W IK H DELI I G N Q ASEV R AA P 85
Cdd:COG1882 4 L AGPTE R T K RLREK L LE - A KP LIDI ERA RLF TE S Y KETEGL P VII RRA K A FS H I L EHKP I Y IK D DELI V G L Q TDKP R KR P 82
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 86 IFPE YTVS W I E K E I D D L AD RP GA GF AV S E E N K RVLH E VC P W W R G Q T VQ D RCYGMFTD E QKGLLET GII K a EGNMTS G DA H 165
Cdd:COG1882 83 IFPE GGIR W V E D E L D A L PT RP QD GF EI S P E D K EIFR E IA P Y W K G K T HN D GVFDAYPE E IRKARKA GII T - GLPDAY G RG H 161
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 166 LAVNFPLL L EK GLDGL RHKVD E RRSRIN LT CLD D LHGDQ F L KA ID IV L EAV TLHI ER F A A LAR KM A ST ET RTS R RD ELL T 245
Cdd:COG1882 162 IIGDYRRV L LY GLDGL IEEAK E KLAELD LT DPE D IEKID F Y KA MI IV C EAV IRLA ER Y A E LAR EL A EK ET DPK R KA ELL E 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 246 M AE N C DVIAHE P PK TFW Q A L Q LCY F IQ L ILQI E S NG HSV S F GR M DQYLYP F Y R RD V E l NQS L DR E H A I ELL HSC W L KL L E 325
Cdd:COG1882 242 I AE I C GFVPAN P AR TFW E A V Q WVW F VY L AAIK E Q NG AAM S L GR F DQYLYP Y Y E RD L E - EGR L TE E E A Q ELL DCF W I KL R E 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 326 V NKI R SGSHSKAS AG S P LYQNV TIGG QN l VS G Q am DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ VI R 405
Cdd:COG1882 321 V RFL R TPEYAELF AG Y P TWVTL TIGG MT - PD G R -- DAVN E LSY L ILE TLRN L PLPE PNL T VR WSEKLPEG FL KKAAE VI S 397
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 406 C G F G M P AFN ND EIV IP EFIKL G IEPQ DA Y DY AAI GC I E TA V G GK W g YRCT G MSF IN F A RVMLA AL EG G R D ATS GK VFL P Q 485
Cdd:COG1882 398 I G T G S P QYE ND DLM IP MLLNK G VTLE DA R DY GIA GC V E PM V P GK Q - MQFF G AGR IN L A KALEY AL NN G V D EKT GK QVG P E 476
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 486 EKALS ag N F NNF DEVM D A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV G 565
Cdd:COG1882 477 TGDPT -- D F LTY DEVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DLNE GGA R Y NFGAIGIA G 554
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 566 IANLGN SL A A V KKLVF EQGAIGQQQ L AA ALA DD F D G lt H E Q LRQ R L I N g APKYGNDDD S VD TLLARAYQ T YI DE LKQ Y HN 645
Cdd:COG1882 555 LSVVAD SL S A I KKLVF DKKKVTMDE L LE ALA AN F E G -- Y E E LRQ L L L N - APKYGNDDD Y VD EIAVELVE T FM DE IRK Y KT 631
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 646 P R ygrgpv GG N Y YAGTSS I SA NVP F G AA T M ATPDGRKA HT PLA E GASP AS G T D HL GPTAV IG SV G KLP TGSILG G V LLNQ 725
Cdd:COG1882 632 Y R ------ GG T Y TLSILT I TS NVP Y G KK T G ATPDGRKA GE PLA D GASP MH G R D KN GPTAV LK SV A KLP YEKATD G I LLNQ 705
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 726 K LN P AT L EN E SDKQK L MI LLRT F F e EHK G W HIQ Y N I V S RETLL E A K KHP DQ Y R DL V VRVAGYSA F F TA LS PDA QDDIIAR 805
Cdd:COG1882 706 K FS P SA L GG E EGIEN L VS LLRT Y F - DLG G H HIQ F N V V D RETLL D A Q KHP EK Y P DL T VRVAGYSA Y F VE LS KEQ QDDIIAR 784
...
gi 489125480 806 TEH 808
Cdd:COG1882 785 TEH 787
PFL-like
pfam02901 Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
11-671
0e+00
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048
Cd Length: 647
Bit Score: 735.59
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 11 D RI K AHK T al IHIVK P PVCT ERA QHY TE M Y QQHLDK - P IPV RRA L AL AHH L AERTIW I KH DELI I G N Q ASEV R AAP I F PE 89
Cdd:pfam02901 6 D VL K ENY T -- LYTGD P SLSW ERA RLL TE S Y KETEGV l P VDI RRA K AL KKI L SHLPGY I RD DELI V G L Q TDKP R KRA I Y PE 83
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 90 YTVS W I E K E I D D L AD RP GA GF AV SEE N K RVLH E VC P W W R G Q T VQDRCYGMF T D E Q K GLL E T GI IK a EGNMTS G DA H LAVN 169
Cdd:pfam02901 84 GGIR W V E D E L D Y L NT RP QD GF EI SEE D K KIFR E IF P Y W K G K T HNEGVFDAY T P E M K AAR E S GI FT - GLPDAY G RG H IIGD 162
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 170 FPLL L EK GLDGL RHKVD E RRSRINLTC l D D LHGDQ F L KA ID I VLE AV TLHI ER F A A LA RKM A ST ET RTS R RD ELL TM AE N 249
Cdd:pfam02901 163 YRRV L LY GLDGL IEEKE E KLAKLDTDP - E D IEKIE F Y KA MI I SCD AV IEYA ER Y A R LA EEL A EQ ET DPK R KA ELL EI AE I 241
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 250 C DVIAHE P PK TF WQ A L Q LCY F IQ L ILQI E S NG HSV S F GR M DQYLYP F Y R RD V E l NQS L DR E H A I EL LHSC W L KL L EV NKI 329
Cdd:pfam02901 242 C GRVPAR P AE TF QE A I Q WFW F VY L AAVK E Q NG AAM S L GR L DQYLYP Y Y E RD L E - EGR L TE E E A Q EL IDCF W I KL R EV RFL 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 330 R SGSHS K AS AG SPLY QN V TIGGQ NL v S G Q am DAVN P LSY A ILE SCGR L RSTQ PNL S VR YHAGMSND FL DACVQ V I R C G F G 409
Cdd:pfam02901 321 R TPEYN K LF AG YDPF QN L TIGGQ GR - D G R -- DAVN K LSY L ILE ALDN L PLPE PNL T VR WSKKLPEE FL KKAAE V S R K G T G 397
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 410 M P AFN ND EIV IP EFIKL G IEPQ DA Y DY AAI GC I E TAVG GK - WGYRC tgm SF IN F A RVMLA AL E GGRD ATS GK VFL P QEKA 488
Cdd:pfam02901 398 S P QYE ND DVM IP ALLNR G VSLE DA R DY GIA GC V E PMKP GK e MQFFG --- AR IN L A KALEY AL N GGRD ELT GK QVG P KTGP 474
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 489 LS ag N F NN F D EVM D A WDT Q IR Y YTRKSIEIEYVVDT M LEENVHDILC SAL V DDCIER A K SIKQ GGA K Y DWVSGLQV G I AN 568
Cdd:pfam02901 475 VT -- E F LS F E EVM E A FKK Q LD Y LADLYVNALNIIHY M HDKYAPEPFL SAL H DDCIER G K DVGI GGA R Y NFSGPQGA G L AN 552
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 569 LGN SL A A V KKLVF EQGAIGQQQ L AA ALA D DF D G lt H E Q LRQ R L I N G APKYGNDDD S VD TLLARAYQ T YI DE LKQ Y H N P R y 648
Cdd:pfam02901 553 VAD SL S A I KKLVF DDKVYTLRE L ED ALA A DF E G -- E E E LRQ D L L N D APKYGNDDD R VD DIAVEVVE T FM DE VRK Y K N Y R - 629
650 660
....*....|....*....|...
gi 489125480 649 grgpv GG NYYAGTSS I SA NVP F G 671
Cdd:pfam02901 630 ----- GG KFTPSLLT I TS NVP Y G 647
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
9-810
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 585.25
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 9 LSD RI KAH KTAL IHIVKP p VCT ERA QH YT EMYQ Q HLDK P IPV RRA L A L A HH L AERT I W I KHD ELI I GN QASEV RA APIF P 88
Cdd:PRK09983 1 MTN RI SRL KTAL FANTRE - ISL ERA LL YT ASHR Q TEGE P VIL RRA K A T A YI L EHVE I S I RDE ELI A GN RTVKP RA GIMS P 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 89 E YTVS W IE KE I D DLAD RP GAG FA V SEE N KR VLH E VC - P W W RGQTVQ D RCY G MF TDE Q K GLLE T G I IKA eg N M T - S G DA H L 166
Cdd:PRK09983 80 E MDPY W LL KE L D QFPT RP QDR FA I SEE D KR IYR E EL f P Y W EKRSMK D FIN G QM TDE V K AATS T Q I FSI -- N Q T d K G QG H I 157
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 167 AVNF P L LL EK GL DG L R hkvderr SRINLT C LDDLHGD q F LK A IDIV LEA VTL HI E R F A A LA RK MA STE T RTS RR D ELLT M 246
Cdd:PRK09983 158 IIDY P R LL NH GL GE L V ------- AQMQQH C QQQPENH - F YQ A ALLL LEA SQK HI L R Y A E LA ET MA ANC T DAQ RR E ELLT I 229
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 247 AE NCDVI A HEP P K TFWQA L QL CYFIQL ILQ I ESN GH S V S F GR M DQY LY PFY RRD ve L N Q SL D REHAI ELL H S C W L K LLEV 326
Cdd:PRK09983 230 AE ISRHN A QHK P Q TFWQA C QL FWYMNI ILQ Y ESN AS S L S L GR F DQY ML PFY QAS -- L T Q GE D PAFLK ELL E S L W V K CNDI 307
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 327 NKI RS G S HSKAS AG S P LYQNVTI GG qnl VSGQAMD AVN P LS YAI L ESCGRLRST QPNL S VR YH A GMSND FL DACVQV IR C 406
Cdd:PRK09983 308 VLL RS T S SARYF AG F P TGYTALL GG --- LTENGRS AVN V LS FLC L DAYQSVQLP QPNL G VR TN A LIDTP FL MKTAET IR L 384
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 407 G F G M P AFN NDE I V I P E F IKL G IEPQ DA Y DY AAI GC I E TAVG G K w G Y RCTGMSFI N FAR VM LAA L --- EG GRDA T sgkvfl 483
Cdd:PRK09983 385 G T G I P QIF NDE V V V P A F LNR G VSLE DA R DY SVV GC V E LSIP G R - T Y GLHDIAMF N LLK VM EIC L hen EG NAAL T ------ 457
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 484 pqekalsagnfnn FDEVMDAWDTQ I RY Y TRKSI E IEYVV D TMLEENVHDI L C S ALVD DC I E RAKS I KQ GGA K Y DW v SG L Q 563
Cdd:PRK09983 458 ------------- YEGLLEQIRAK I SH Y ITLMV E GSNIC D IGHRDWAPVP L L S SFIS DC L E KGRD I TD GGA R Y NF - SG V Q 523
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 564 - V GIANL GN SL A A V K KL VF E Q GAIGQQQ L AAA L ADD F DGLTH E QL R Q RLIN GAP KYGND D D S VD TLL A RAYQT Y ID E LKQ 642
Cdd:PRK09983 524 g I GIANL SD SL H A L K GM VF D Q QRLSFDE L LSV L KAN F ATPEG E KV R A RLIN RFE KYGND I D E VD NIS A ELLRH Y CK E VEK 603
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 643 Y H NPR ygrgpv GG NYYA G TSSI SA N VP F G AATM ATPDGR K A HTP LA E G A - SP AS G T D HL GPTAV IG SV G KL PTGSILG G V 721
Cdd:PRK09983 604 Y Q NPR ------ GG YFTP G SYTV SA H VP L G SVVG ATPDGR F A GEQ LA D G G l SP ML G Q D AQ GPTAV LK SV S KL DNTLLSN G T 677
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 722 LLN Q K LN PATLE N E SDKQ KL MIL LR T F F e EH K GW HIQ Y N I V SRE TL L EA KKH P DQ Y RD LVVRVAGYSAFF TA LS PDA QDD 801
Cdd:PRK09983 678 LLN V K FT PATLE G E AGLR KL ADF LR A F T - QL K LQ HIQ F N V V NAD TL R EA QQR P QD Y AG LVVRVAGYSAFF VE LS KEI QDD 756
....*....
gi 489125480 802 II A RT E H M L 810
Cdd:PRK09983 757 II R RT A H Q L 765
choline_CutC
TIGR04394 choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and ...
9-806
1.39e-147
choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and benzylsuccinate synthases, are glycine radical enzymes that appear to act as choline TMA-lyase, that is, to perform a C-N bond cleavage turning choline into trimethylamine (TMA) plus acetaldehyde. The gene symbol is cutC, for choline utilization. The activase, CutD, is a radical SAM enzyme. [Energy metabolism, Amino acids and amines]
Pssm-ID: 275187 [Multi-domain]
Cd Length: 789
Bit Score: 452.72
E-value: 1.39e-147
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 9 LSD R IKAH K TALIH i VK P PVCTE RA QHY TE MYQQHLDK P IPVR RA L A LAHHLAERTIW I KH DELI I G NQASEV RA APIF P 88
Cdd:TIGR04394 1 PTE R LVRL K ENYLK - QV P SITIY RA RAI TE IAKENPGM P KILL RA K A FRKCCETAPLV I QD DELI V G APCGAP RA GAFS P 79
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 89 EYTVS W IEK E I D DLAD RP GAG F AV SEE N K RVLH E VC - P W W R G QTVQDR C YGMF td EQK G LL E tgi IKA E GNMTSGDA H l A 167
Cdd:TIGR04394 80 DIAWR W LRD E L D TIGT RP QDP F YI SEE D K KIMR E EI f P F W E G KSLDEY C EDQY -- REA G VW E --- LSG E SFVSDCSY H - A 153
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 168 VN ----- F P ---- L L LE KG LDGLRHKVD E RRSRINLTCLD D LHGDQ F L K AIDIVL E A V TLHIE R FAAL A RKM A ST E TRTS 238
Cdd:TIGR04394 154 VN gggds N P gydv I L MK KG MLDIQAEAK E HLAELSYENPE D IDKIY F Y K SVIDTT E G V MIYAK R LSEY A AEL A AK E QDPK 233
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 239 R RD EL LTM AE - N CD V I AH e P P K TFW Q A L Q LCYFIQLI L QI E S N GHSV S F GR M DQY L YPFY RR D V E l NQSLDREH A I EL LH 317
Cdd:TIGR04394 234 R KA EL EKI AE v N AR V P AH - K P R TFW E A I Q SVWTVESL L VV E E N QTGM S I GR V DQY M YPFY KA D I E - AGRMTEYE A F EL AG 311
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 318 SCWL K LL E VNKIR S GSH SK AS AG SPLYQ N VTI GG qnl V SGQAM DA V N P L S Y AILESCGRLRST QP N L SV R Y H AGMSNDF L 397
Cdd:TIGR04394 312 CMLI K MS E MMWLT S EGG SK FF AG YQPFV N MCV GG --- V TREGG DA T N D L T Y LLMDAVRHVKVY QP S L AC R I H NKSPQKY L 388
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 398 DAC V Q V I R C G F G M PA FNN D EIV I PEFIKL G IEPQ DA Y DY AAI GC I E TAVG G KW g Y RC T GMSFINFARVMLAA L EG G RDAT 477
Cdd:TIGR04394 389 KKI V D V V R A G M G F PA CHF D DAH I KMMLAK G VSIE DA R DY CLM GC V E PQKS G RL - Y QW T STAYTQWPICIELV L NH G VPLW 467
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 478 S GK VFL P QEKA LS ag N F NNFDEVMD A WDT QI R Y Y T RK S ieiey V V D T MLEEN VH - D I ---- L C S ALVDD C I E RA K SIKQ G 552
Cdd:TIGR04394 468 Y GK QVC P DTGD LS -- Q F DTYEKFDA A VKE QI K Y I T KW S ----- A V A T VISQR VH r D L apkp L M S LMYEG C M E KG K DVSA G 540
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 553 GA K Y DWVS G L - QV G I A NLGN S L AA V KKLV FEQGAIGQQ QL AA AL ADD F D G lt H EQ L R QRLIN g APKYGNDDD SV D TLL A - 630
Cdd:TIGR04394 541 GA M Y NFGP G V v WS G L A TYAD S M AA I KKLV YDDKKYTLE QL NE AL KAN F E G -- Y EQ I R ADCLD - APKYGNDDD YA D LIA A d 617
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 631 --------- R A Y Q T YIDE L KQ yhnprygrgpvggnyya GT S SIS A N V PFG AA T M A TPD GR K A H TPL AE G A SP AS G T D HL G 701
Cdd:TIGR04394 618 lvnftereh R K Y K T LYSH L SH ----------------- GT L SIS N N T PFG QL T G A SAN GR L A W TPL SD G I SP TQ G A D FK G 680
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 702 PTA V I G SV G K LPTG S ILG G VLL N Q KL NPAT L ENESDKQK L MI LLRT FFEEHK G w HI Q Y N IVSR ETLL E A KK HP DQ YRDLV 781
Cdd:TIGR04394 681 PTA I I K SV S K MAND S MNI G MVH N F KL MSGL L DTPEGENG L IT LLRT ASILGN G - EM Q F N YLDN ETLL D A QQ HP EK YRDLV 759
810 820
....*....|....*....|....*
gi 489125480 782 VRVAGYSAFF TA L SP D A QD D II A RT 806
Cdd:TIGR04394 760 VRVAGYSAFF VE L CK D V QD E II S RT 784
PFL1
cd01678 Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, ...
241-808
1.31e-54
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate in which two cysteines and one glycine form radicals that are required for catalysis. PFL has a ten-stranded alpha/beta barrel domain that is structurally similar to those of all three ribonucleotide reductase (RNR) classes as well as benzylsuccinate synthase and B12-independent glycerol dehydratase.
Pssm-ID: 153087 [Multi-domain]
Cd Length: 738
Bit Score: 201.44
E-value: 1.31e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 241 D EL LT MA ENCDVIAHE P PKTFWQ A L Q LC YF IQ L ILQI E S NG HSV S F GR MDQY L YPFYR RD VE l NQSLDREH A I EL LHSCW 320
Cdd:cd01678 218 K EL KQ MA ASYGLDISR P ATNAQE A I Q WT YF GY L AAIK E Q NG AAM S L GR VSTF L DIYIE RD LK - AGTITEAE A Q EL IDQFI 296
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 321 L KL LE V NKI R SGSHSKASA G S P LYQNVT IGG QNL v S G QAM da V NPL S YAI L ESCGR L - RSTQ PNL S V RYHAGMSND F LDA 399
Cdd:cd01678 297 M KL RM V RFL R TPEYNELFS G D P TWVTES IGG MGN - D G RSL -- V TKT S FRF L NTLYN L g PAPE PNL T V LWSEKLPEN F KRF 373
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 400 C VQ V ------ I R cgfgmpa FN ND EIVI P EF iklgiepqd AY D YAA I G C IET A VG - GK ---- W G Y R C tgmsfi N F A RVM L A 468
Cdd:cd01678 374 C AK V sidtss I Q ------- YE ND DLMR P DW --------- GG D DYG I A C CVS A MR i GK qmqf F G A R A ------ N L A KAL L Y 431
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 469 A LE GGRD ATS G KVFL P QEKAL s AGNFNNF DEVM DAW D TQIRYYTRKSIEIEYVVDT M LEENVHDI L CS AL V D DCIE R A ks 548
Cdd:cd01678 432 A IN GGRD EKT G DQVG P DIEPI - TSDYLDY DEVM ENY D KSMDWLADTYVNALNIIHY M HDKYAYEA L QM AL H D TDVR R T -- 508
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 549 ikqggakydwvsg LQV GIA N L GN --- SL A A V K -- K LVFEQGAI G qqqlaaa LA D DF D gltheqlrqrl I N G A - P K YGNDD 622
Cdd:cd01678 509 ------------- MAF GIA G L SV aad SL S A I K ya K VKPIRDED G ------- LA V DF E ----------- I E G D f P R YGNDD 557
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 623 D SV D TLLARAYQ T YIDE L KQYHNP R ygrgpvgg N YYAGT S -- S I SA NV PF G AA T MA TPDGR K A HT P L A E GA S P AS G T D HL 700
Cdd:cd01678 558 D RA D DIAVWVVK T FMNK L RKHKTY R -------- N AEPTQ S vl T I TS NV VY G KK T GN TPDGR R A GE P F A P GA N P MH G R D KK 629
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 701 G PT A VIG SV G KLP TGSILG G VLLNQKLN P AT L ENESDKQK -- L MIL L RTF F E e HK G W H IQY N IVS RETLL E A KK HP DQ Y R 778
Cdd:cd01678 630 G AL A SLA SV A KLP YRDAND G ISNTFSIV P NA L GKTDEERI dn L VGI L DGY F T - KG G H H LNV N VLN RETLL D A ME HP EK Y P 708
570 580 590
....*....|....*....|....*....|
gi 489125480 779 D L VV RV A GY SAF F TA L SPDA Q D D I I A RT E H 808
Cdd:cd01678 709 Q L TI RV S GY AVN F VK L TREQ Q L D V I S RT F H 738
Gly_radical
pfam01228 Glycine radical;
687-792
2.13e-40
Glycine radical;
Pssm-ID: 426140 [Multi-domain]
Cd Length: 106
Bit Score: 143.85
E-value: 2.13e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 687 L A E G A SP AS G T D HL GPTAV IG SVGK LPTGSI L G G VL LNQK LN PA T L ENESDKQ -- K L MI L LR T F F ee HK G W H I Q Y N I V S R 764
Cdd:pfam01228 1 V A P G I SP SH G A D FE GPTAV LN SVGK IDYEVE L D G IS LNQK FL PA V L GYYDEEG ya N L NT L ID T Y F -- EG G H H L Q F N V V D R 78
90 100
....*....|....*....|....*...
gi 489125480 765 ETL LE A K KHP DQ Y R DL V VRV A GYSA F F T 792
Cdd:pfam01228 79 ETL PD A Q KHP EK Y P DL T VRV S GYSA N F V 106
PRK11127
PRK11127 autonomous glycyl radical cofactor GrcA; Provisional
754-808
3.33e-15
autonomous glycyl radical cofactor GrcA; Provisional
Pssm-ID: 182983 [Multi-domain]
Cd Length: 127
Bit Score: 72.95
E-value: 3.33e-15
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*
gi 489125480 754 G W H IQY N IVS RETL LE A K KHP DQ Y RD L VV RV A GY SAF F TA L S P DA Q D D I IART EH 808
Cdd:PRK11127 70 G Q H LNV N VLR RETL ED A V KHP EK Y PQ L TI RV S GY AVR F NS L T P EQ Q R D V IART FT 124
RNR_PFL
cd00576 Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and ...
242-467
1.84e-13
Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and pyruvate formate lyase (PFL) are believed to have diverged from a common ancestor. They have a structurally similar ten-stranded alpha-beta barrel domain that hosts the active site, and are radical enzymes. RNRs are found in all organisms and provide the only mechanism by which nucleotides are converted to deoxynucleotides. RNRs are separated into three classes based on their metallocofactor usage. Class I RNRs use a diiron-tyrosyl radical while Class II RNRs use coenzyme B12 (adenosylcobalamin, AdoCbl). Class III RNRs use an FeS cluster and S-adenosylmethionine to generate a glycyl radical. PFL, an essential enzyme in anaerobic bacteria, catalyzes the conversion of pyruvate and CoA to acteylCoA and formate in a mechanism that uses a glycyl radical.
Pssm-ID: 153083 [Multi-domain]
Cd Length: 401
Bit Score: 72.95
E-value: 1.84e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 242 ELLTMAENCDVIAH E PP K TFWQ A L Q LC Y F I QLILQIES NG HS VSF G R MDQY L Y P FYR RD VEL n Q S LDREH A I E LLHSCW L 321
Cdd:cd00576 29 DYGDSLDPGIKGVN E TA K SINE A I Q KT Y Q I IALAASNQ NG GG VSF A R ASSI L S P YGS RD YAK - G S GTETD A V E AADAFN L 107
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 322 K L L EV NK irsgshska SA G SPLYQNVT IGG QNLVS G Q a MDAVNP L SY A ILESCGRLRSTQ PNLSVR YHAGMSN ------ D 395
Cdd:cd00576 108 A L K EV GQ --------- GN G RTGAATGF IGG VHKGK G D - KISQEF L NL A LANGGEGIPLNF PNLSVR VSSDKPG ilvkav E 177
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489125480 396 FLDACVQVI R c GF G M P AFN NDE I -------------- V I P ----------- E FIK L GIEPQ D AYDYAAIGC I E T AVG G KW 450
Cdd:cd00576 178 LKQLIAEEA R - KT G S P GIF NDE L cnlvslnlarimek A I N gsmdvvleele E LAF L AVRAL D CVIDSHDER I P T IEL G GD 256
250
....*....|....*..
gi 489125480 451 GY R CT G MSFINF A RVML 467
Cdd:cd00576 257 ER R TV G LGIAGV A DLLI 273
Blast search parameters
Data Source:
Precalculated data, version = cdd.v.3.21
Preset Options: Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
