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Conserved domains on  [gi|489306016|ref|WP_003213454|]
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MULTISPECIES: Tim44 domain-containing protein [Pseudomonas]

Protein Classification

Tim44 domain-containing protein( domain architecture ID 10008544)

Tim44 domain-containing protein similar to mitochondrial 39S ribosomal protein L45 and to mitochondrial import inner membrane translocase subunit Tim44, an essential component of the eukaryotic PAM complex which is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Tim44 COG4395
Predicted lipid-binding transport protein, Tim44 family [Lipid transport and metabolism];
99-290 6.45e-36

Predicted lipid-binding transport protein, Tim44 family [Lipid transport and metabolism];


:

Pssm-ID: 443521  Cd Length: 186  Bit Score: 126.94  E-value: 6.45e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489306016  99 GMQIFDILIMAVIA-FLIFRFIAARRRKQQEHMAPAGApmqrevfeqkpaamgsifggsaapaaaRPVINAPAWFNEERF 177
Cdd:COG4395    5 GAFLLDILILALIAaFLILRFLRSGRRTGFERPAAAGL---------------------------AAIAAAPPGFDVEAF 57

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489306016 178 VEAARSHFQSLQQHWDANEMDKIAEFVTPQLLEFLKRERADLGDGFQ---STYIDDLKVQLEGVDDRADKTIATLTFSGV 254
Cdd:COG4395   58 LRGAKAAFEMIQAAWDAGDLEDLRELLTPEVYAEFQAAIAEREAAGEtneTTFVGILDAELVDAELEGDEAEVTVRFVGE 137

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 489306016 255 SKTSRFDQG-----------EVFSESWNMERPQGENQP-WLVAGIRQN 290
Cdd:COG4395  138 LREATRDADgevvegdpaaaEEFREVWTFARPLGSGDPnWLLAGIQQA 185
 
Name Accession Description Interval E-value
Tim44 COG4395
Predicted lipid-binding transport protein, Tim44 family [Lipid transport and metabolism];
99-290 6.45e-36

Predicted lipid-binding transport protein, Tim44 family [Lipid transport and metabolism];


Pssm-ID: 443521  Cd Length: 186  Bit Score: 126.94  E-value: 6.45e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489306016  99 GMQIFDILIMAVIA-FLIFRFIAARRRKQQEHMAPAGApmqrevfeqkpaamgsifggsaapaaaRPVINAPAWFNEERF 177
Cdd:COG4395    5 GAFLLDILILALIAaFLILRFLRSGRRTGFERPAAAGL---------------------------AAIAAAPPGFDVEAF 57

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489306016 178 VEAARSHFQSLQQHWDANEMDKIAEFVTPQLLEFLKRERADLGDGFQ---STYIDDLKVQLEGVDDRADKTIATLTFSGV 254
Cdd:COG4395   58 LRGAKAAFEMIQAAWDAGDLEDLRELLTPEVYAEFQAAIAEREAAGEtneTTFVGILDAELVDAELEGDEAEVTVRFVGE 137

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 489306016 255 SKTSRFDQG-----------EVFSESWNMERPQGENQP-WLVAGIRQN 290
Cdd:COG4395  138 LREATRDADgevvegdpaaaEEFREVWTFARPLGSGDPnWLLAGIQQA 185
Tim44 smart00978
Tim44 is an essential component of the machinery that mediates the translocation of ...
 160-287 1.25e-22

Tim44 is an essential component of the machinery that mediates the translocation of nuclear-encoded proteins across the mitochondrial inner membrane; Tim44 is thought to bind phospholipids of the mitochondrial inner membrane both by electrostatic interactions and by penetrating the polar head group region.


Pssm-ID: 214950  Cd Length: 147  Bit Score: 91.23  E-value: 1.25e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489306016   160 AAARPVINAPAWFNEERFVEAARSHFQSLQQHWDANEMDKIAEFVTPQLLEFLK---RERADLGDGFQSTYIDDLKVQLE 236
Cdd:smart00978   2 KALREIKRIDPGFDTERFLEGAKDAFPMIQEAWDRGDLETLRELLTPEMYNELAaqiAEREARGLFVENKLLDIDVVVLD 81

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489306016   237 ----GVDDRADKTIATLTFSGVSKTSRFD-QGEV----------FSESWNMERPQGENQPWLVAGI 287
Cdd:smart00978  82 aallEAWQEGDGAVITVRFHGQQIDVTRDaKGGVvvggedkpkeFTEVWTFTRDLNGDPNWRLSGI 147
Tim44 pfam04280
Tim44-like domain; Tim44 is an essential component of the machinery that mediates the ...
 156-287 3.13e-10

Tim44-like domain; Tim44 is an essential component of the machinery that mediates the translocation of nuclear-encoded proteins across the mitochondrial inner membrane. Tim44 is thought to bind phospholipids of the mitochondrial inner membrane both by electrostatic interactions and by penetrating the polar head group region. This family includes the C-terminal region of Tim44 that has been shown to form a stable proteolytic fragment in yeast. This region is also found in a set of smaller bacterial proteins. The molecular function of the bacterial members of this family is unknown but transport seems likely. The crystal structure of the C terminal of Tim44 has revealed a large hydrophobic pocket which might play an important role in interacting with the acyl chains of lipid molecules in the mitochondrial membrane.


Pssm-ID: 427835  Cd Length: 145  Bit Score: 57.24  E-value: 3.13e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489306016  156 SAAPAAARpviNAPAWFNEERFVEAARSHFQSLQQHWDANEMDKIAEFVTPQLLEFLK---RERADLGDGFQSTYIDDLK 232
Cdd:pfam04280   1 ASALRAIK---AIDPSFDVEEFLEGAKEAFEPILEAYAKGDLETLRPLLTEEVFEEFAaaiDEREAQGLTNDLEIVDIRE 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489306016  233 VQLEGVDDRADKTIATLTFSG--VSKTSRFDQGEV----------FSESWNMERPQGENQP-WLVAGI 287
Cdd:pfam04280  78 VDIAEAKMEGGTAVITVRFRAqqIDVTRDDATGEVvegdpddpveVTEVWTFTRDLGNPDPnWRLVAT 145
SA1362_fam NF041554
SA1362 family protein; Members of this family include SA1362 from Staphylococcus aureus. The ...
 79-128 9.23e-03

SA1362 family protein; Members of this family include SA1362 from Staphylococcus aureus. The N-terminal 50 amino acids are highly hydrophobic and suggest two transmembrane alpha-helical regions. The C-terminal region rich in basic residues, variable in length, and often somewhat repetitive. The family is widespread in Gram-positive species.


Pssm-ID: 469439  Cd Length: 70  Bit Score: 34.34  E-value: 9.23e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 489306016  79 LAGIAAGGLLASMFM--GGGFQgmQIFDILIMAVIAFLIFRFIAARRRKQQE 128
Cdd:NF041554   8 IIGLAAIGLVSQLFTnpGGLLR--SILIIAVIGAIIYFIYYFFFLRRRGGGE 57
 
Name Accession Description Interval E-value
Tim44 COG4395
Predicted lipid-binding transport protein, Tim44 family [Lipid transport and metabolism];
99-290 6.45e-36

Predicted lipid-binding transport protein, Tim44 family [Lipid transport and metabolism];


Pssm-ID: 443521  Cd Length: 186  Bit Score: 126.94  E-value: 6.45e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489306016  99 GMQIFDILIMAVIA-FLIFRFIAARRRKQQEHMAPAGApmqrevfeqkpaamgsifggsaapaaaRPVINAPAWFNEERF 177
Cdd:COG4395    5 GAFLLDILILALIAaFLILRFLRSGRRTGFERPAAAGL---------------------------AAIAAAPPGFDVEAF 57

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489306016 178 VEAARSHFQSLQQHWDANEMDKIAEFVTPQLLEFLKRERADLGDGFQ---STYIDDLKVQLEGVDDRADKTIATLTFSGV 254
Cdd:COG4395   58 LRGAKAAFEMIQAAWDAGDLEDLRELLTPEVYAEFQAAIAEREAAGEtneTTFVGILDAELVDAELEGDEAEVTVRFVGE 137

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 489306016 255 SKTSRFDQG-----------EVFSESWNMERPQGENQP-WLVAGIRQN 290
Cdd:COG4395  138 LREATRDADgevvegdpaaaEEFREVWTFARPLGSGDPnWLLAGIQQA 185
Tim44 smart00978
Tim44 is an essential component of the machinery that mediates the translocation of ...
 160-287 1.25e-22

Tim44 is an essential component of the machinery that mediates the translocation of nuclear-encoded proteins across the mitochondrial inner membrane; Tim44 is thought to bind phospholipids of the mitochondrial inner membrane both by electrostatic interactions and by penetrating the polar head group region.


Pssm-ID: 214950  Cd Length: 147  Bit Score: 91.23  E-value: 1.25e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489306016   160 AAARPVINAPAWFNEERFVEAARSHFQSLQQHWDANEMDKIAEFVTPQLLEFLK---RERADLGDGFQSTYIDDLKVQLE 236
Cdd:smart00978   2 KALREIKRIDPGFDTERFLEGAKDAFPMIQEAWDRGDLETLRELLTPEMYNELAaqiAEREARGLFVENKLLDIDVVVLD 81

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489306016   237 ----GVDDRADKTIATLTFSGVSKTSRFD-QGEV----------FSESWNMERPQGENQPWLVAGI 287
Cdd:smart00978  82 aallEAWQEGDGAVITVRFHGQQIDVTRDaKGGVvvggedkpkeFTEVWTFTRDLNGDPNWRLSGI 147
Tim44 pfam04280
Tim44-like domain; Tim44 is an essential component of the machinery that mediates the ...
 156-287 3.13e-10

Tim44-like domain; Tim44 is an essential component of the machinery that mediates the translocation of nuclear-encoded proteins across the mitochondrial inner membrane. Tim44 is thought to bind phospholipids of the mitochondrial inner membrane both by electrostatic interactions and by penetrating the polar head group region. This family includes the C-terminal region of Tim44 that has been shown to form a stable proteolytic fragment in yeast. This region is also found in a set of smaller bacterial proteins. The molecular function of the bacterial members of this family is unknown but transport seems likely. The crystal structure of the C terminal of Tim44 has revealed a large hydrophobic pocket which might play an important role in interacting with the acyl chains of lipid molecules in the mitochondrial membrane.


Pssm-ID: 427835  Cd Length: 145  Bit Score: 57.24  E-value: 3.13e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489306016  156 SAAPAAARpviNAPAWFNEERFVEAARSHFQSLQQHWDANEMDKIAEFVTPQLLEFLK---RERADLGDGFQSTYIDDLK 232
Cdd:pfam04280   1 ASALRAIK---AIDPSFDVEEFLEGAKEAFEPILEAYAKGDLETLRPLLTEEVFEEFAaaiDEREAQGLTNDLEIVDIRE 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489306016  233 VQLEGVDDRADKTIATLTFSG--VSKTSRFDQGEV----------FSESWNMERPQGENQP-WLVAGI 287
Cdd:pfam04280  78 VDIAEAKMEGGTAVITVRFRAqqIDVTRDDATGEVvegdpddpveVTEVWTFTRDLGNPDPnWRLVAT 145
RseC COG3086
RseC, positive regulator of sigma E activity [Signal transduction mechanisms];
78-128 2.09e-03

RseC, positive regulator of sigma E activity [Signal transduction mechanisms];


Pssm-ID: 442320  Cd Length: 149  Bit Score: 37.94  E-value: 2.09e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 489306016  78 PLAGIAAGGLLASMFMGGGFQGMQIFDILIMAVIAFLIFRFIAARRRKQQE 128
Cdd:COG3086   85 PLLGLLLGALLGQALGPGGSELLAALGGLLGLALGFLLARRLSRRLAKKSK 135
SA1362_fam NF041554
SA1362 family protein; Members of this family include SA1362 from Staphylococcus aureus. The ...
 79-128 9.23e-03

SA1362 family protein; Members of this family include SA1362 from Staphylococcus aureus. The N-terminal 50 amino acids are highly hydrophobic and suggest two transmembrane alpha-helical regions. The C-terminal region rich in basic residues, variable in length, and often somewhat repetitive. The family is widespread in Gram-positive species.


Pssm-ID: 469439  Cd Length: 70  Bit Score: 34.34  E-value: 9.23e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 489306016  79 LAGIAAGGLLASMFM--GGGFQgmQIFDILIMAVIAFLIFRFIAARRRKQQE 128
Cdd:NF041554   8 IIGLAAIGLVSQLFTnpGGLLR--SILIIAVIGAIIYFIYYFFFLRRRGGGE 57
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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