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Conserved domains on  [gi|489307504|ref|WP_003214939|]
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MULTISPECIES: 2-hydroxymuconate tautomerase [Bacillus]

Protein Classification

tautomerase family protein( domain architecture ID 1755)

tautomerase family protein similar to Homo sapiens macrophage migration inhibitory factor and D-dopachrome decarboxylase-like protein

CATH:  3.30.429.10
Gene Ontology:  GO:0006725|GO:0016853
PubMed:  29184004|12051677|18695941
SCOP:  3001770

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
4Oxalocrotonate_Tautomerase super family cl00235
4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The ...
 1-60 4.47e-24

4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The structure is a homohexamer that is arranged as a trimer of dimers. The hexamer contains six active sites, each formed by residues from three monomers, two from one dimer and the third from a neighboring monomer. Each monomer is a beta-alpha-beta fold with two small beta strands at the C-terminus that fold back on themselves. A pair of monomers form a dimer with two-fold symmetry, consisting of a 4-stranded beta sheet with two helices on one side and two additional small beta strands at each end. The dimers are assembled around a 3-fold axis of rotation to form a hexamer, with the short beta strands from each dimer contacting the neighboring dimers.


The actual alignment was detected with superfamily member PRK02220:

Pssm-ID: 444774  Cd Length: 61  Bit Score: 84.81  E-value: 4.47e-24
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489307504  1 MPIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMSD 60
Cdd:PRK02220  1 MPYVHIKLIEGRTEEQLKALVKDVTAAVSKNTGAPAEHIHVIINEMSKNHYAVGGKRLSD 60
 
Name Accession Description Interval E-value
PRK02220 PRK02220
4-oxalocrotonate tautomerase; Provisional
 1-60 4.47e-24

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 235013  Cd Length: 61  Bit Score: 84.81  E-value: 4.47e-24
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489307504  1 MPIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMSD 60
Cdd:PRK02220  1 MPYVHIKLIEGRTEEQLKALVKDVTAAVSKNTGAPAEHIHVIINEMSKNHYAVGGKRLSD 60
PptA COG1942
Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites ...
 2-59 1.35e-21

Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 441545  Cd Length: 58  Bit Score: 78.27  E-value: 1.35e-21
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 489307504  2 PIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMS 59
Cdd:COG1942   1 PIINIKLFEGRTPEQKRALAKAVTDAVVEVLGKPPESVSVVIEEVPPENWGIGGKLLS 58
4Oxalocrotonate_Tautomerase cd00491
4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The ...
 2-59 1.63e-21

4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The structure is a homohexamer that is arranged as a trimer of dimers. The hexamer contains six active sites, each formed by residues from three monomers, two from one dimer and the third from a neighboring monomer. Each monomer is a beta-alpha-beta fold with two small beta strands at the C-terminus that fold back on themselves. A pair of monomers form a dimer with two-fold symmetry, consisting of a 4-stranded beta sheet with two helices on one side and two additional small beta strands at each end. The dimers are assembled around a 3-fold axis of rotation to form a hexamer, with the short beta strands from each dimer contacting the neighboring dimers.


Pssm-ID: 238274  Cd Length: 58  Bit Score: 78.20  E-value: 1.63e-21
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 489307504  2 PIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMS 59
Cdd:cd00491   1 PFVQIYILEGRTDEQKRELIERVTEAVSEILGAPEATIVVIIDEMPKENWGIGGESAS 58
Tautomerase pfam01361
Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that ...
 2-60 1.91e-18

Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that catalyzes the ketonization of 2-hydroxymuconate to 2-oxo-3-hexenedioate.


Pssm-ID: 396090  Cd Length: 60  Bit Score: 70.40  E-value: 1.91e-18
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 489307504   2 PIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMSD 60
Cdd:pfam01361  1 PFVNIKLFEGRTDEQKKELIERVTDALVEVLGAPRSAIVVIIEEVPPENWGVGGESVGD 59
taut TIGR00013
4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in ...
 2-61 1.07e-17

4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in which each monomer is very small, at about 62 amino acids. Pro-1 of the mature protein serves as a general base. The enzyme functions in meta-cleavage pathways of aromatic hydrocarbon catabolism. Because several Arg residues located near the active site in the crystal structure of Pseudomonas putida are not conserved among all members of this family, because the literature describes a general role in the isomerization of beta,gamma-unsaturated enones to their alpha,beta-isomers, and because of the presence of fairly distantly related paralogs in Campylobacter jejuni, the family is regarded as not necessarily uniform in function. [Energy metabolism, Other]


Pssm-ID: 129125  Cd Length: 63  Bit Score: 68.60  E-value: 1.07e-17
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 489307504   2 PIVTVQML-EGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMSDL 61
Cdd:TIGR00013  1 PFVNIYILkEGRTDEQKRQLIEGVTEAMAETLGANLESIVVIIDEMPKNNYGIGGELVSDR 61
 
Name Accession Description Interval E-value
PRK02220 PRK02220
4-oxalocrotonate tautomerase; Provisional
 1-60 4.47e-24

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 235013  Cd Length: 61  Bit Score: 84.81  E-value: 4.47e-24
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489307504  1 MPIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMSD 60
Cdd:PRK02220  1 MPYVHIKLIEGRTEEQLKALVKDVTAAVSKNTGAPAEHIHVIINEMSKNHYAVGGKRLSD 60
PptA COG1942
Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites ...
 2-59 1.35e-21

Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 441545  Cd Length: 58  Bit Score: 78.27  E-value: 1.35e-21
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 489307504  2 PIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMS 59
Cdd:COG1942   1 PIINIKLFEGRTPEQKRALAKAVTDAVVEVLGKPPESVSVVIEEVPPENWGIGGKLLS 58
4Oxalocrotonate_Tautomerase cd00491
4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The ...
 2-59 1.63e-21

4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The structure is a homohexamer that is arranged as a trimer of dimers. The hexamer contains six active sites, each formed by residues from three monomers, two from one dimer and the third from a neighboring monomer. Each monomer is a beta-alpha-beta fold with two small beta strands at the C-terminus that fold back on themselves. A pair of monomers form a dimer with two-fold symmetry, consisting of a 4-stranded beta sheet with two helices on one side and two additional small beta strands at each end. The dimers are assembled around a 3-fold axis of rotation to form a hexamer, with the short beta strands from each dimer contacting the neighboring dimers.


Pssm-ID: 238274  Cd Length: 58  Bit Score: 78.20  E-value: 1.63e-21
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 489307504  2 PIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMS 59
Cdd:cd00491   1 PFVQIYILEGRTDEQKRELIERVTEAVSEILGAPEATIVVIIDEMPKENWGIGGESAS 58
Tautomerase pfam01361
Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that ...
 2-60 1.91e-18

Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that catalyzes the ketonization of 2-hydroxymuconate to 2-oxo-3-hexenedioate.


Pssm-ID: 396090  Cd Length: 60  Bit Score: 70.40  E-value: 1.91e-18
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 489307504   2 PIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMSD 60
Cdd:pfam01361  1 PFVNIKLFEGRTDEQKKELIERVTDALVEVLGAPRSAIVVIIEEVPPENWGVGGESVGD 59
taut TIGR00013
4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in ...
 2-61 1.07e-17

4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in which each monomer is very small, at about 62 amino acids. Pro-1 of the mature protein serves as a general base. The enzyme functions in meta-cleavage pathways of aromatic hydrocarbon catabolism. Because several Arg residues located near the active site in the crystal structure of Pseudomonas putida are not conserved among all members of this family, because the literature describes a general role in the isomerization of beta,gamma-unsaturated enones to their alpha,beta-isomers, and because of the presence of fairly distantly related paralogs in Campylobacter jejuni, the family is regarded as not necessarily uniform in function. [Energy metabolism, Other]


Pssm-ID: 129125  Cd Length: 63  Bit Score: 68.60  E-value: 1.07e-17
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 489307504   2 PIVTVQML-EGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMSDL 61
Cdd:TIGR00013  1 PFVNIYILkEGRTDEQKRQLIEGVTEAMAETLGANLESIVVIIDEMPKNNYGIGGELVSDR 61
PRK00745 PRK00745
4-oxalocrotonate tautomerase; Provisional
 1-60 2.11e-17

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 179107  Cd Length: 62  Bit Score: 67.82  E-value: 2.11e-17
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489307504  1 MPIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMSD 60
Cdd:PRK00745  1 MPTFHIELFEGRTVEQKRKLVEEITRVTVETLGCPPESVDIIITDVKRENWATGGKLWSD 60
PRK01964 PRK01964
4-oxalocrotonate tautomerase; Provisional
 1-61 1.76e-13

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 179355  Cd Length: 64  Bit Score: 57.89  E-value: 1.76e-13
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 489307504  1 MPIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGKRMSDL 61
Cdd:PRK01964  1 MPIVQIQLLEGRPEEKIKNLIREVTEAISATLDVPKERVRVIVNEVPSSHWGVAGVPKSEL 61
PRK02289 PRK02289
4-oxalocrotonate tautomerase; Provisional
 1-56 3.55e-09

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 179406  Cd Length: 60  Bit Score: 47.24  E-value: 3.55e-09
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 489307504  1 MPIVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEHYAVAGK 56
Cdd:PRK02289  1 MPFVRIDLFEGRSQEQKNALAREVTEVVSRIAKAPKEAIHVFINDMPEGTYYPQGE 56
HpaF COG3232
5-carboxymethyl-2-hydroxymuconate isomerase [Amino acid transport and metabolism];
4-52 2.76e-06

5-carboxymethyl-2-hydroxymuconate isomerase [Amino acid transport and metabolism];


Pssm-ID: 442464 [Multi-domain]  Cd Length: 121  Bit Score: 41.00  E-value: 2.76e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 489307504   4 VTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEK---VSVIIEEMKKEHYA 52
Cdd:COG3232   63 VTLRILAGRSDEQKQALSEALFAALRAHLAPVFARyvaLSVEIREIDRASYA 114
CHMI cd00580
5-carboxymethyl-2-hydroxymuconate isomerase (CHMI) is a trimeric enzyme catalyzing the ...
 4-49 6.66e-06

5-carboxymethyl-2-hydroxymuconate isomerase (CHMI) is a trimeric enzyme catalyzing the isomerization of the unsaturated ketone 5-(carboxymethyl)-2-hydroxymuconate to 5-(carboxymethyl)-2-oxo-3-hexene-1,6-dionate. This is one step in the homoprotocatechuate pathway, one of the microbial meta-fission pathways that degrade aromatic carbon sources to citric acid cycle intermediates. Despite the structural similarity of CHMI with 4-oxalocrotonate tautomerase (4-OT) and macrophage migration inhibitory factor (MIF), there is no significant sequence similarity among these protein families, and therefore, they are not combined in one hierarchy.


Pssm-ID: 238324 [Multi-domain]  Cd Length: 113  Bit Score: 39.86  E-value: 6.66e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 489307504   4 VTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEK----VSVIIEEMKKE 49
Cdd:cd00580   63 VTLRILAGRSEEQKQELSEALLAALRAHLAPVFAKrylsLSVEIRELDPA 112
Tautomerase_2 pfam14552
Tautomerase enzyme;
3-50 6.53e-05

Tautomerase enzyme;


Pssm-ID: 434035  Cd Length: 82  Bit Score: 36.77  E-value: 6.53e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 489307504   3 IVTVQMLEGRTDEQKRALVEKVTDAVVETTGAGAEKVSVIIEEMKKEH 50
Cdd:pfam14552 31 IIQITLFAGRSVEQKKALYRALAERLAAKLGIRPEDVFIVLVENPKEN 78
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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