NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|489521720|ref|WP_003426509|]
View 

glycosyltransferase [Clostridioides difficile]

Protein Classification

glycosyltransferase family 32 protein( domain architecture ID 1000645)

glycosyltransferase family 32 protein similar to Schizosaccharomyces pombe inositol phosphoceramide mannosyltransferase 1 that catalyzes the addition of mannosyl to phosphorylinositol ceramide (IPC)

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
OCH1 COG3774
Mannosyltransferase OCH1 or related enzyme [Cell wall/membrane/envelope biogenesis];
1-133 9.71e-50

Mannosyltransferase OCH1 or related enzyme [Cell wall/membrane/envelope biogenesis];


:

Pssm-ID: 442988 [Multi-domain]  Cd Length: 136  Bit Score: 159.28  E-value: 9.71e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489521720   1 MIPKKIHYVWFGG-PKGNIENICINSWKEKLPEYEIVEWNEKNFDIEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQ 79
Cdd:COG3774    2 MIPKIIHQTWFGDkELPELVQRCIASWKKLNPDWEYRLWDDENFDDFIKEHGPEYLEAYYKLKKGAARADLFRLLLLYKY 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 489521720  80 GGVYMDTDMQILKDITPLLENNRLICGYED-DREYINGAIIGVEKGHPFLKDLLE 133
Cdd:COG3774   82 GGIYLDIDVECLKPLDPLLDGDDLFLGYEDpPPGIISNGFIAAEPGHPFLKKALE 136
Gb3_synth super family cl04600
Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of ...
 122-211 1.43e-03

Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of eukaryotic cell membranes. They consist of a hydrophilic carbohydrate moiety linked to a hydrophobic ceramide tail embedded within the lipid bilayer of the membrane. Lactosylceramide, Gal1,4Glc1Cer (LacCer), is the common synthetic precursor to the majority of GSL found in vertebrates. Alpha 1.4-glycosyltransferases utilize UDP donors and transfer the sugar to a beta-linked acceptor. This region appears to be confined to higher eukaryotes. No function has been yet assigned to this region.


The actual alignment was detected with superfamily member pfam04572:

Pssm-ID: 428017  Cd Length: 130  Bit Score: 37.61  E-value: 1.43e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489521720  122 EKGHPF----LKDLLEYYEKEVLTSSLftiPKIMTHLMEK--NYKKIDPNNYEE--GIRVYDKEYFYPFGFKED---FTP 190
Cdd:pfam04572   3 DKGHPFlelcLRDFVENYNGDEWGHNG---PGLLTRVLKKfcNTSDLKAMERVRcrGFTVLPPEAFYPIPYPNWkrfFEP 79

                          90       100
                  ....*....|....*....|....*..
gi 489521720  191 EC------ITENTFGIHWWGKSWAKKR 211
Cdd:pfam04572  80 EDleetmkLTKDSYAVHLWNKLSKGEP 106
 
Name Accession Description Interval E-value
OCH1 COG3774
Mannosyltransferase OCH1 or related enzyme [Cell wall/membrane/envelope biogenesis];
1-133 9.71e-50

Mannosyltransferase OCH1 or related enzyme [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442988 [Multi-domain]  Cd Length: 136  Bit Score: 159.28  E-value: 9.71e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489521720   1 MIPKKIHYVWFGG-PKGNIENICINSWKEKLPEYEIVEWNEKNFDIEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQ 79
Cdd:COG3774    2 MIPKIIHQTWFGDkELPELVQRCIASWKKLNPDWEYRLWDDENFDDFIKEHGPEYLEAYYKLKKGAARADLFRLLLLYKY 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 489521720  80 GGVYMDTDMQILKDITPLLENNRLICGYED-DREYINGAIIGVEKGHPFLKDLLE 133
Cdd:COG3774   82 GGIYLDIDVECLKPLDPLLDGDDLFLGYEDpPPGIISNGFIAAEPGHPFLKKALE 136
Gly_transf_sug pfam04488
Glycosyltransferase sugar-binding region containing DXD motif; The DXD motif is a short ...
 19-103 3.05e-13

Glycosyltransferase sugar-binding region containing DXD motif; The DXD motif is a short conserved motif found in many families of glycosyltransferases, which add a range of different sugars to other sugars, phosphates and proteins. DXD-containing glycosyltransferases all use nucleoside diphosphate sugars as donors and require divalent cations, usually manganese. The DXD motif is expected to play a carbohydrate binding role in sugar-nucleoside diphosphate and manganese dependent glycosyltransferases.


Pssm-ID: 398274 [Multi-domain]  Cd Length: 93  Bit Score: 63.51  E-value: 3.05e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489521720   19 ENICINSWKEKLPEYEIVEWNEKNF---DIEKEIKGNKFLEECYKRkLWAFI--SDYTRIKVLYEQGGVYMDTDMQILKD 93
Cdd:pfam04488   4 VMKAIESLIKLHPDYCYVVLSDDLDfalDINFLKSDTPWFLEAYSL-LPLFIakSDLLRYAILYKYGGIYLDTDVIPLKS 82

                          90
                  ....*....|
gi 489521720   94 ITPLLENNRL 103
Cdd:pfam04488  83 LNSIGAQERF 92
Gb3_synth pfam04572
Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of ...
 122-211 1.43e-03

Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of eukaryotic cell membranes. They consist of a hydrophilic carbohydrate moiety linked to a hydrophobic ceramide tail embedded within the lipid bilayer of the membrane. Lactosylceramide, Gal1,4Glc1Cer (LacCer), is the common synthetic precursor to the majority of GSL found in vertebrates. Alpha 1.4-glycosyltransferases utilize UDP donors and transfer the sugar to a beta-linked acceptor. This region appears to be confined to higher eukaryotes. No function has been yet assigned to this region.


Pssm-ID: 428017  Cd Length: 130  Bit Score: 37.61  E-value: 1.43e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489521720  122 EKGHPF----LKDLLEYYEKEVLTSSLftiPKIMTHLMEK--NYKKIDPNNYEE--GIRVYDKEYFYPFGFKED---FTP 190
Cdd:pfam04572   3 DKGHPFlelcLRDFVENYNGDEWGHNG---PGLLTRVLKKfcNTSDLKAMERVRcrGFTVLPPEAFYPIPYPNWkrfFEP 79

                          90       100
                  ....*....|....*....|....*..
gi 489521720  191 EC------ITENTFGIHWWGKSWAKKR 211
Cdd:pfam04572  80 EDleetmkLTKDSYAVHLWNKLSKGEP 106
 
Name Accession Description Interval E-value
OCH1 COG3774
Mannosyltransferase OCH1 or related enzyme [Cell wall/membrane/envelope biogenesis];
1-133 9.71e-50

Mannosyltransferase OCH1 or related enzyme [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442988 [Multi-domain]  Cd Length: 136  Bit Score: 159.28  E-value: 9.71e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489521720   1 MIPKKIHYVWFGG-PKGNIENICINSWKEKLPEYEIVEWNEKNFDIEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQ 79
Cdd:COG3774    2 MIPKIIHQTWFGDkELPELVQRCIASWKKLNPDWEYRLWDDENFDDFIKEHGPEYLEAYYKLKKGAARADLFRLLLLYKY 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 489521720  80 GGVYMDTDMQILKDITPLLENNRLICGYED-DREYINGAIIGVEKGHPFLKDLLE 133
Cdd:COG3774   82 GGIYLDIDVECLKPLDPLLDGDDLFLGYEDpPPGIISNGFIAAEPGHPFLKKALE 136
Gly_transf_sug pfam04488
Glycosyltransferase sugar-binding region containing DXD motif; The DXD motif is a short ...
 19-103 3.05e-13

Glycosyltransferase sugar-binding region containing DXD motif; The DXD motif is a short conserved motif found in many families of glycosyltransferases, which add a range of different sugars to other sugars, phosphates and proteins. DXD-containing glycosyltransferases all use nucleoside diphosphate sugars as donors and require divalent cations, usually manganese. The DXD motif is expected to play a carbohydrate binding role in sugar-nucleoside diphosphate and manganese dependent glycosyltransferases.


Pssm-ID: 398274 [Multi-domain]  Cd Length: 93  Bit Score: 63.51  E-value: 3.05e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489521720   19 ENICINSWKEKLPEYEIVEWNEKNF---DIEKEIKGNKFLEECYKRkLWAFI--SDYTRIKVLYEQGGVYMDTDMQILKD 93
Cdd:pfam04488   4 VMKAIESLIKLHPDYCYVVLSDDLDfalDINFLKSDTPWFLEAYSL-LPLFIakSDLLRYAILYKYGGIYLDTDVIPLKS 82

                          90
                  ....*....|
gi 489521720   94 ITPLLENNRL 103
Cdd:pfam04488  83 LNSIGAQERF 92
Caps_synth pfam05704
Capsular polysaccharide synthesis protein; This family consists of several capsular ...
 1-138 1.46e-06

Capsular polysaccharide synthesis protein; This family consists of several capsular polysaccharide proteins. Capsular polysaccharide (CPS) is a major virulence factor in Streptococcus pneumoniae.


Pssm-ID: 368570  Cd Length: 278  Bit Score: 48.04  E-value: 1.46e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489521720    1 MIPKKIHYVWFGGpkgnIEN------ICINSWKEKLPEYEIVEWNEKNfdIEKEIKGNKFLEECYKRKLW--AFISDYTR 72
Cdd:pfam05704  45 QNNKIIWICWFQG----IDNapeivkKCIDSVKKNAPDYEVVLLTKDN--ISEYVDIPDFIIEKFEKGKIsrAHFSDILR 118

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489521720   73 IKVLYEQGGVYMDTDMQILKDITPLLENNRL--ICGYEDDREYIN-----GAIIGVEKGHPFLKD----LLEYYEKE 138
Cdd:pfam05704 119 LNLLAKYGGVWIDATIYCTGDLPNELLDSDFftFSSSELDSNKISisrwtGFFLAAKKNNPLLSTlrdlLLEYWKKY 195
Gb3_synth pfam04572
Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of ...
 122-211 1.43e-03

Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of eukaryotic cell membranes. They consist of a hydrophilic carbohydrate moiety linked to a hydrophobic ceramide tail embedded within the lipid bilayer of the membrane. Lactosylceramide, Gal1,4Glc1Cer (LacCer), is the common synthetic precursor to the majority of GSL found in vertebrates. Alpha 1.4-glycosyltransferases utilize UDP donors and transfer the sugar to a beta-linked acceptor. This region appears to be confined to higher eukaryotes. No function has been yet assigned to this region.


Pssm-ID: 428017  Cd Length: 130  Bit Score: 37.61  E-value: 1.43e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489521720  122 EKGHPF----LKDLLEYYEKEVLTSSLftiPKIMTHLMEK--NYKKIDPNNYEE--GIRVYDKEYFYPFGFKED---FTP 190
Cdd:pfam04572   3 DKGHPFlelcLRDFVENYNGDEWGHNG---PGLLTRVLKKfcNTSDLKAMERVRcrGFTVLPPEAFYPIPYPNWkrfFEP 79

                          90       100
                  ....*....|....*....|....*..
gi 489521720  191 EC------ITENTFGIHWWGKSWAKKR 211
Cdd:pfam04572  80 EDleetmkLTKDSYAVHLWNKLSKGEP 106
TcdA_TcdB pfam12919
TcdA/TcdB catalytic glycosyltransferase domain; This domain represents the N-terminal ...
 4-42 1.51e-03

TcdA/TcdB catalytic glycosyltransferase domain; This domain represents the N-terminal glycosyltransferase from a set of toxins found in some bacteria. This domain in TcdB glycosylates the host RhoA protein.


Pssm-ID: 372382 [Multi-domain]  Cd Length: 382  Bit Score: 39.05  E-value: 1.51e-03
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 489521720    4 KKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKN 42
Cdd:pfam12919   1 KNLHFVWIGGAIPDIQEDYINVWKDVNEGYNINLWYDSN 39
TcdA_TcdB pfam12919
TcdA/TcdB catalytic glycosyltransferase domain; This domain represents the N-terminal ...
 41-88 9.78e-03

TcdA/TcdB catalytic glycosyltransferase domain; This domain represents the N-terminal glycosyltransferase from a set of toxins found in some bacteria. This domain in TcdB glycosylates the host RhoA protein.


Pssm-ID: 372382 [Multi-domain]  Cd Length: 382  Bit Score: 36.74  E-value: 9.78e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 489521720   41 KNFDIEKEIKG-NKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDM 88
Cdd:pfam12919 146 RDIKSFKQAELvNNYEQELFLRGNLAAASDIVRLLALKELGGLYTDVDM 194
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH