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Conserved domains on  [gi|489525741|ref|WP_003430501|]
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cell wall-binding repeat-containing protein [Clostridioides difficile]

Protein Classification

cell wall-binding repeat-containing protein( domain architecture ID 10514007)

cell wall-binding repeat-containing protein similar to Clostridium difficile S-layer protein SlpA, cell wall protein V (CwpV), and Cwp66; the cell wall binding repeat 2 is found in mutiple copies in proteins

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
LytB COG2247
Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane ...
 7-310 8.69e-26

Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane/envelope biogenesis];


:

Pssm-ID: 441848 [Multi-domain]  Cd Length: 472  Bit Score: 109.81  E-value: 8.69e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741   7 VLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLIADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNK 86
Cdd:COG2247  157 VAAAAAGGVGRAAGGRAAAAAAAAAAAAAGGAAGAVAIAKAGDSADTVVLARGDNFADALAAGPLAAALNAPILLTPSDS 236

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741  87 IPNETKQRLK--NVKKIYIIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVsgIKKVDEVILTNAYkGEAD 164
Cdd:COG2247  237 LPPATLAELKrlGPKKVYILGGTAAVSEAVEDQLKALGITVERIAGADRYETAAAIAKEL--GPDADTVVLASGE-DFPD 313

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741 165 TISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRLGGSDRYDTNKKVIKEFYPDASEFYLS 244
Cdd:COG2247  314 ALSAAAAAAAAAAAAAAAAAAVAAAAAAAAAAAAVAAAAGAALVAASAAAAAAAAAAGVAAAVAAGGAAGAAADAGAAAA 393

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489525741 245 DGYDLVNALTGSTIAKENPIVLVSESSDKSILAGADKITRLGSISDSVYNKCVSAAQNNGDSSTKG 310
Cdd:COG2247  394 AAAAAAAAAAAAAAAAAAAAAAAVAAAGAAAAAAAAGGGAAAALLAAAGAGALAAAAAGAAVATGG 459
Glucosaminidase pfam01832
Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase; This family includes ...
 349-419 5.88e-07

Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase; This family includes Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase EC:3.2.1.96. As well as the flageller protein J that has been shown to hydrolyse peptidoglycan.


:

Pssm-ID: 460354 [Multi-domain]  Cd Length: 91  Bit Score: 47.57  E-value: 5.88e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489525741  349 PILYDTAVQEGVNPVLAVAQSAKETGFcnFGGVLDASFKNPCGLKTSVGGS---DTD----KNAHSRFDTWEEGILAQ 419
Cdd:pfam01832   2 PAAIEAAKKYGIPASVLLAQAALESGW--GTSRLAKESNNLFGIKASWKGKvayDTDevtvAARFRKYDSVEESIRDY 77
 
Name Accession Description Interval E-value
LytB COG2247
Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane ...
 7-310 8.69e-26

Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441848 [Multi-domain]  Cd Length: 472  Bit Score: 109.81  E-value: 8.69e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741   7 VLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLIADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNK 86
Cdd:COG2247  157 VAAAAAGGVGRAAGGRAAAAAAAAAAAAAGGAAGAVAIAKAGDSADTVVLARGDNFADALAAGPLAAALNAPILLTPSDS 236

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741  87 IPNETKQRLK--NVKKIYIIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVsgIKKVDEVILTNAYkGEAD 164
Cdd:COG2247  237 LPPATLAELKrlGPKKVYILGGTAAVSEAVEDQLKALGITVERIAGADRYETAAAIAKEL--GPDADTVVLASGE-DFPD 313

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741 165 TISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRLGGSDRYDTNKKVIKEFYPDASEFYLS 244
Cdd:COG2247  314 ALSAAAAAAAAAAAAAAAAAAVAAAAAAAAAAAAVAAAAGAALVAASAAAAAAAAAAGVAAAVAAGGAAGAAADAGAAAA 393

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489525741 245 DGYDLVNALTGSTIAKENPIVLVSESSDKSILAGADKITRLGSISDSVYNKCVSAAQNNGDSSTKG 310
Cdd:COG2247  394 AAAAAAAAAAAAAAAAAAAAAAAVAAAGAAAAAAAAGGGAAAALLAAAGAGALAAAAAGAAVATGG 459
CW_binding_2 pfam04122
ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide ...
 32-113 8.55e-15

ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide variety of bacterial cell surface proteins. It has been show the three tandem repeats of the CWB2 domain are essential for correct anchoring to the cell wall. It was shown that in SlpA and Cwp2 that these domains were essential for the binding of PSII an anionic teichoic acid-like component of the cell wall. The structure of the Cwp8 and Cwp6 proteins shows that this domain forms a trimeric arrangement with each domain adopting a structure with some similarity to the Toprim fold. A groove containing many conserved residues was predicted to be the site of the PSII molecule.


Pssm-ID: 461185 [Multi-domain]  Cd Length: 80  Bit Score: 69.48  E-value: 8.55e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741   32 IKGNNIYETAGLIADKKSYDTAIMVNMDNSiADGLSASGLAGAVDAPILLAQKNKiPNETKQRLKNvKKIYIIGKELSIS 111
Cdd:pfam04122   2 LAGSDRYETAAKIAKELGWDTVVVASGENF-ADALSAAPLAAKKNAPILLTDKNS-LDSLKKALKA-ELVYILGGTGVIS 78


                  ..
gi 489525741  112 KS 113
Cdd:pfam04122  79 DS 80
Glucosaminidase pfam01832
Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase; This family includes ...
 349-419 5.88e-07

Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase; This family includes Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase EC:3.2.1.96. As well as the flageller protein J that has been shown to hydrolyse peptidoglycan.


Pssm-ID: 460354 [Multi-domain]  Cd Length: 91  Bit Score: 47.57  E-value: 5.88e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489525741  349 PILYDTAVQEGVNPVLAVAQSAKETGFcnFGGVLDASFKNPCGLKTSVGGS---DTD----KNAHSRFDTWEEGILAQ 419
Cdd:pfam01832   2 PAAIEAAKKYGIPASVLLAQAALESGW--GTSRLAKESNNLFGIKASWKGKvayDTDevtvAARFRKYDSVEESIRDY 77
 
Name Accession Description Interval E-value
LytB COG2247
Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane ...
 7-310 8.69e-26

Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441848 [Multi-domain]  Cd Length: 472  Bit Score: 109.81  E-value: 8.69e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741   7 VLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLIADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNK 86
Cdd:COG2247  157 VAAAAAGGVGRAAGGRAAAAAAAAAAAAAGGAAGAVAIAKAGDSADTVVLARGDNFADALAAGPLAAALNAPILLTPSDS 236

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741  87 IPNETKQRLK--NVKKIYIIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVsgIKKVDEVILTNAYkGEAD 164
Cdd:COG2247  237 LPPATLAELKrlGPKKVYILGGTAAVSEAVEDQLKALGITVERIAGADRYETAAAIAKEL--GPDADTVVLASGE-DFPD 313

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741 165 TISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRLGGSDRYDTNKKVIKEFYPDASEFYLS 244
Cdd:COG2247  314 ALSAAAAAAAAAAAAAAAAAAVAAAAAAAAAAAAVAAAAGAALVAASAAAAAAAAAAGVAAAVAAGGAAGAAADAGAAAA 393

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489525741 245 DGYDLVNALTGSTIAKENPIVLVSESSDKSILAGADKITRLGSISDSVYNKCVSAAQNNGDSSTKG 310
Cdd:COG2247  394 AAAAAAAAAAAAAAAAAAAAAAAVAAAGAAAAAAAAGGGAAAALLAAAGAGALAAAAAGAAVATGG 459
CW_binding_2 pfam04122
ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide ...
 32-113 8.55e-15

ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide variety of bacterial cell surface proteins. It has been show the three tandem repeats of the CWB2 domain are essential for correct anchoring to the cell wall. It was shown that in SlpA and Cwp2 that these domains were essential for the binding of PSII an anionic teichoic acid-like component of the cell wall. The structure of the Cwp8 and Cwp6 proteins shows that this domain forms a trimeric arrangement with each domain adopting a structure with some similarity to the Toprim fold. A groove containing many conserved residues was predicted to be the site of the PSII molecule.


Pssm-ID: 461185 [Multi-domain]  Cd Length: 80  Bit Score: 69.48  E-value: 8.55e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741   32 IKGNNIYETAGLIADKKSYDTAIMVNMDNSiADGLSASGLAGAVDAPILLAQKNKiPNETKQRLKNvKKIYIIGKELSIS 111
Cdd:pfam04122   2 LAGSDRYETAAKIAKELGWDTVVVASGENF-ADALSAAPLAAKKNAPILLTDKNS-LDSLKKALKA-ELVYILGGTGVIS 78


                  ..
gi 489525741  112 KS 113
Cdd:pfam04122  79 DS 80
CW_binding_2 pfam04122
ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide ...
 217-291 6.47e-11

ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide variety of bacterial cell surface proteins. It has been show the three tandem repeats of the CWB2 domain are essential for correct anchoring to the cell wall. It was shown that in SlpA and Cwp2 that these domains were essential for the binding of PSII an anionic teichoic acid-like component of the cell wall. The structure of the Cwp8 and Cwp6 proteins shows that this domain forms a trimeric arrangement with each domain adopting a structure with some similarity to the Toprim fold. A groove containing many conserved residues was predicted to be the site of the PSII molecule.


Pssm-ID: 461185 [Multi-domain]  Cd Length: 80  Bit Score: 58.31  E-value: 6.47e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741  217 RLGGSDRYDTNKKVIKEFYPDASefYLSDGYDLVNALTGSTIAKEN--PIVLVSESSDKSILAG--ADKITRLG---SIS 289
Cdd:pfam04122   1 RLAGSDRYETAAKIAKELGWDTV--VVASGENFADALSAAPLAAKKnaPILLTDKNSLDSLKKAlkAELVYILGgtgVIS 78


                  ..
gi 489525741  290 DS 291
Cdd:pfam04122  79 DS 80
CW_binding_2 pfam04122
ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide ...
 127-208 9.49e-10

ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide variety of bacterial cell surface proteins. It has been show the three tandem repeats of the CWB2 domain are essential for correct anchoring to the cell wall. It was shown that in SlpA and Cwp2 that these domains were essential for the binding of PSII an anionic teichoic acid-like component of the cell wall. The structure of the Cwp8 and Cwp6 proteins shows that this domain forms a trimeric arrangement with each domain adopting a structure with some similarity to the Toprim fold. A groove containing many conserved residues was predicted to be the site of the PSII molecule.


Pssm-ID: 461185 [Multi-domain]  Cd Length: 80  Bit Score: 54.84  E-value: 9.49e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489525741  127 RLGGDDRIKTSYSVAKEVSGikkvDEVILTNAYkGEADTISAAPVSVRDIAPIVLTDG---KSVPFSTSNVKTYAVGGSI 203
Cdd:pfam04122   1 RLAGSDRYETAAKIAKELGW----DTVVVASGE-NFADALSAAPLAAKKNAPILLTDKnslDSLKKALKAELVYILGGTG 75


                  ....*
gi 489525741  204 SMSTS 208
Cdd:pfam04122  76 VISDS 80
Glucosaminidase pfam01832
Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase; This family includes ...
 349-419 5.88e-07

Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase; This family includes Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase EC:3.2.1.96. As well as the flageller protein J that has been shown to hydrolyse peptidoglycan.


Pssm-ID: 460354 [Multi-domain]  Cd Length: 91  Bit Score: 47.57  E-value: 5.88e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489525741  349 PILYDTAVQEGVNPVLAVAQSAKETGFcnFGGVLDASFKNPCGLKTSVGGS---DTD----KNAHSRFDTWEEGILAQ 419
Cdd:pfam01832   2 PAAIEAAKKYGIPASVLLAQAALESGW--GTSRLAKESNNLFGIKASWKGKvayDTDevtvAARFRKYDSVEESIRDY 77
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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