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Conserved domains on  [gi|489606730|ref|WP_003511171|]
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MULTISPECIES: sulfite-sensing transcriptional repressor BigR [Rhizobium/Agrobacterium group]

Protein Classification

ArsR/SmtB family transcription factor( domain architecture ID 16677510)

ArsR (Arsenical Resistance Operon Repressor)/SmtB family transcription factor is a winged-helix-turn-helix transcriptional regulator that may be metal-sensing and modulate the expression of genes associated with metal homeostasis

Gene Ontology:  GO:0003700|GO:0006355|GO:0003677
PubMed:  10473770|28436329|12829264|28512703|30191942|15808743

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
sulf_sens_BigR NF040642
sulfite-sensing transcriptional repressor BigR; BigR (biofilm growth-associated repressor) is ...
 19-113 2.86e-56

sulfite-sensing transcriptional repressor BigR; BigR (biofilm growth-associated repressor) is a winged-helix DNA-binding protein active as a transcriptional regulator for Blh, a bifunctional sulfur transferase/dioxygenase found in a number of plant-associated bacteria. Oxidative conditions that allow disulfide bond formation between the invariant residues Cys42 and Cys108 cause BigR to lose DNA-binding transcriptional repressor activity.


:

Pssm-ID: 468610  Cd Length: 95  Bit Score: 169.55  E-value: 2.86e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489606730  19 AEMEERAVEVAILLKTLAHPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEK 98
Cdd:NF040642   1 AEMEARAGEVADLLKTLAHPARLMLVCTLVEGEYSVGELEEKLDIHQPTLSQQLTVLREAGIVETRREGKQIFYRLTEEK 80

                         90
                 ....*....|....*
gi 489606730  99 AAQLIEALYTIFCAP 113
Cdd:NF040642  81 AAQLVGALYTIFCAK 95
 
Name Accession Description Interval E-value
sulf_sens_BigR NF040642
sulfite-sensing transcriptional repressor BigR; BigR (biofilm growth-associated repressor) is ...
 19-113 2.86e-56

sulfite-sensing transcriptional repressor BigR; BigR (biofilm growth-associated repressor) is a winged-helix DNA-binding protein active as a transcriptional regulator for Blh, a bifunctional sulfur transferase/dioxygenase found in a number of plant-associated bacteria. Oxidative conditions that allow disulfide bond formation between the invariant residues Cys42 and Cys108 cause BigR to lose DNA-binding transcriptional repressor activity.


Pssm-ID: 468610  Cd Length: 95  Bit Score: 169.55  E-value: 2.86e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489606730  19 AEMEERAVEVAILLKTLAHPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEK 98
Cdd:NF040642   1 AEMEARAGEVADLLKTLAHPARLMLVCTLVEGEYSVGELEEKLDIHQPTLSQQLTVLREAGIVETRREGKQIFYRLTEEK 80

                         90
                 ....*....|....*
gi 489606730  99 AAQLIEALYTIFCAP 113
Cdd:NF040642  81 AAQLVGALYTIFCAK 95
ArsR COG0640
DNA-binding transcriptional regulator, ArsR family [Transcription];
20-110 9.52e-30

DNA-binding transcriptional regulator, ArsR family [Transcription];


Pssm-ID: 440405 [Multi-domain]  Cd Length: 92  Bit Score: 102.28  E-value: 9.52e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489606730  20 EMEERAVEVAILLKTLAHPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEKA 99
Cdd:COG0640    2 SETKELEELAELFKALADPTRLRILRLLAEGELCVGELAEALGLSQSTVSHHLKVLREAGLVTSRREGRWVYYRLADEAL 81

                         90
                 ....*....|.
gi 489606730 100 AQLIEALYTIF 110
Cdd:COG0640   82 AELLEWLLELL 92
HTH_metalloreg NF033788
metalloregulator ArsR/SmtB family transcription factor; Transcriptional repressors that sense ...
 31-103 1.96e-26

metalloregulator ArsR/SmtB family transcription factor; Transcriptional repressors that sense toxic heavy metals such as arsenic or cadmium, and are released from DNA so that resistance factors will be expressed, include ArsR, SmtB, ZiaR, CadC, CadX, KmtR, etc. However, some members of this family, including the sporulation delaying system autorepressor SdpR and its family (see NF033789), may lack metal-binding cites and instead regulate other cellular processes.


Pssm-ID: 411368 [Multi-domain]  Cd Length: 76  Bit Score: 93.29  E-value: 1.96e-26
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 489606730  31 LLKTLAHPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEKAAQLI 103
Cdd:NF033788   4 LFKALADPTRLRILELLAEGELCVCELAEALGLSQSAVSQHLKVLRDAGLVTSRREGRWVYYRLADEHVAELL 76
HTH_ARSR smart00418
helix_turn_helix, Arsenical Resistance Operon Repressor;
47-106 4.25e-18

helix_turn_helix, Arsenical Resistance Operon Repressor;


Pssm-ID: 197713 [Multi-domain]  Cd Length: 66  Bit Score: 71.86  E-value: 4.25e-18
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489606730    47 LAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEKAAQLIEAL 106
Cdd:smart00418   6 LAEGELCVCELAEILGLSQSTVSHHLKKLREAGLVESRREGKRVYYSLTDEKVADLLEEL 65
HTH_ARSR cd00090
Arsenical Resistance Operon Repressor and similar prokaryotic, metal regulated homodimeric ...
 32-106 8.54e-17

Arsenical Resistance Operon Repressor and similar prokaryotic, metal regulated homodimeric repressors. ARSR subfamily of helix-turn-helix bacterial transcription regulatory proteins (winged helix topology). Includes several proteins that appear to dissociate from DNA in the presence of metal ions.


Pssm-ID: 238042 [Multi-domain]  Cd Length: 78  Bit Score: 68.86  E-value: 8.54e-17
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489606730  32 LKTLAHPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTE-EKAAQLIEAL 106
Cdd:cd00090    1 LKALSDPTRLRILRLLLEGPLTVSELAERLGLSQSTVSRHLKKLEEAGLVESRREGRRVYYSLTDaERLLALLESL 76
HTH_20 pfam12840
Helix-turn-helix domain; This domain represents a DNA-binding Helix-turn-helix domain found in ...
 32-88 5.26e-10

Helix-turn-helix domain; This domain represents a DNA-binding Helix-turn-helix domain found in transcriptional regulatory proteins.


Pssm-ID: 432824 [Multi-domain]  Cd Length: 61  Bit Score: 51.00  E-value: 5.26e-10
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 489606730   32 LKTLAHPARL-MLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAK 88
Cdd:pfam12840   4 AEALADPTRLrILRALVGDEPLTASELARRLDISRNTLSYHLRKLEEAGLVEVVREGR 61
PRK10141 PRK10141
DNA-binding transcriptional repressor ArsR; Provisional
 31-107 9.70e-09

DNA-binding transcriptional repressor ArsR; Provisional


Pssm-ID: 236654  Cd Length: 117  Bit Score: 49.43  E-value: 9.70e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489606730  31 LLKTLAHPARLMLACTLAQ-GEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEK---AAQLIEAL 106
Cdd:PRK10141   9 LFKILSDETRLGIVLLLREsGELCVCDLCTALDQSQPKISRHLALLRESGLLLDRKQGKWVHYRLSPHIpawAAKIIEQA 88


                 .
gi 489606730 107 Y 107
Cdd:PRK10141  89 W 89
 
Name Accession Description Interval E-value
sulf_sens_BigR NF040642
sulfite-sensing transcriptional repressor BigR; BigR (biofilm growth-associated repressor) is ...
 19-113 2.86e-56

sulfite-sensing transcriptional repressor BigR; BigR (biofilm growth-associated repressor) is a winged-helix DNA-binding protein active as a transcriptional regulator for Blh, a bifunctional sulfur transferase/dioxygenase found in a number of plant-associated bacteria. Oxidative conditions that allow disulfide bond formation between the invariant residues Cys42 and Cys108 cause BigR to lose DNA-binding transcriptional repressor activity.


Pssm-ID: 468610  Cd Length: 95  Bit Score: 169.55  E-value: 2.86e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489606730  19 AEMEERAVEVAILLKTLAHPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEK 98
Cdd:NF040642   1 AEMEARAGEVADLLKTLAHPARLMLVCTLVEGEYSVGELEEKLDIHQPTLSQQLTVLREAGIVETRREGKQIFYRLTEEK 80

                         90
                 ....*....|....*
gi 489606730  99 AAQLIEALYTIFCAP 113
Cdd:NF040642  81 AAQLVGALYTIFCAK 95
ArsR COG0640
DNA-binding transcriptional regulator, ArsR family [Transcription];
20-110 9.52e-30

DNA-binding transcriptional regulator, ArsR family [Transcription];


Pssm-ID: 440405 [Multi-domain]  Cd Length: 92  Bit Score: 102.28  E-value: 9.52e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489606730  20 EMEERAVEVAILLKTLAHPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEKA 99
Cdd:COG0640    2 SETKELEELAELFKALADPTRLRILRLLAEGELCVGELAEALGLSQSTVSHHLKVLREAGLVTSRREGRWVYYRLADEAL 81

                         90
                 ....*....|.
gi 489606730 100 AQLIEALYTIF 110
Cdd:COG0640   82 AELLEWLLELL 92
HTH_metalloreg NF033788
metalloregulator ArsR/SmtB family transcription factor; Transcriptional repressors that sense ...
 31-103 1.96e-26

metalloregulator ArsR/SmtB family transcription factor; Transcriptional repressors that sense toxic heavy metals such as arsenic or cadmium, and are released from DNA so that resistance factors will be expressed, include ArsR, SmtB, ZiaR, CadC, CadX, KmtR, etc. However, some members of this family, including the sporulation delaying system autorepressor SdpR and its family (see NF033789), may lack metal-binding cites and instead regulate other cellular processes.


Pssm-ID: 411368 [Multi-domain]  Cd Length: 76  Bit Score: 93.29  E-value: 1.96e-26
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 489606730  31 LLKTLAHPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEKAAQLI 103
Cdd:NF033788   4 LFKALADPTRLRILELLAEGELCVCELAEALGLSQSAVSQHLKVLRDAGLVTSRREGRWVYYRLADEHVAELL 76
HTH_ARSR smart00418
helix_turn_helix, Arsenical Resistance Operon Repressor;
47-106 4.25e-18

helix_turn_helix, Arsenical Resistance Operon Repressor;


Pssm-ID: 197713 [Multi-domain]  Cd Length: 66  Bit Score: 71.86  E-value: 4.25e-18
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489606730    47 LAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEKAAQLIEAL 106
Cdd:smart00418   6 LAEGELCVCELAEILGLSQSTVSHHLKKLREAGLVESRREGKRVYYSLTDEKVADLLEEL 65
HTH_ARSR cd00090
Arsenical Resistance Operon Repressor and similar prokaryotic, metal regulated homodimeric ...
 32-106 8.54e-17

Arsenical Resistance Operon Repressor and similar prokaryotic, metal regulated homodimeric repressors. ARSR subfamily of helix-turn-helix bacterial transcription regulatory proteins (winged helix topology). Includes several proteins that appear to dissociate from DNA in the presence of metal ions.


Pssm-ID: 238042 [Multi-domain]  Cd Length: 78  Bit Score: 68.86  E-value: 8.54e-17
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489606730  32 LKTLAHPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTE-EKAAQLIEAL 106
Cdd:cd00090    1 LKALSDPTRLRILRLLLEGPLTVSELAERLGLSQSTVSRHLKKLEEAGLVESRREGRRVYYSLTDaERLLALLESL 76
HTH_20 pfam12840
Helix-turn-helix domain; This domain represents a DNA-binding Helix-turn-helix domain found in ...
 32-88 5.26e-10

Helix-turn-helix domain; This domain represents a DNA-binding Helix-turn-helix domain found in transcriptional regulatory proteins.


Pssm-ID: 432824 [Multi-domain]  Cd Length: 61  Bit Score: 51.00  E-value: 5.26e-10
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 489606730   32 LKTLAHPARL-MLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAK 88
Cdd:pfam12840   4 AEALADPTRLrILRALVGDEPLTASELARRLDISRNTLSYHLRKLEEAGLVEVVREGR 61
PRK10141 PRK10141
DNA-binding transcriptional repressor ArsR; Provisional
 31-107 9.70e-09

DNA-binding transcriptional repressor ArsR; Provisional


Pssm-ID: 236654  Cd Length: 117  Bit Score: 49.43  E-value: 9.70e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489606730  31 LLKTLAHPARLMLACTLAQ-GEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTEEK---AAQLIEAL 106
Cdd:PRK10141   9 LFKILSDETRLGIVLLLREsGELCVCDLCTALDQSQPKISRHLALLRESGLLLDRKQGKWVHYRLSPHIpawAAKIIEQA 88


                 .
gi 489606730 107 Y 107
Cdd:PRK10141  89 W 89
HTH_5 pfam01022
Bacterial regulatory protein, arsR family; Members of this family contains a DNA binding ...
 37-81 1.42e-08

Bacterial regulatory protein, arsR family; Members of this family contains a DNA binding 'helix-turn-helix' motif. This family includes other proteins which are not included in the Prosite definition.


Pssm-ID: 425993 [Multi-domain]  Cd Length: 45  Bit Score: 47.05  E-value: 1.42e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 489606730   37 HPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVV 81
Cdd:pfam01022   1 DPTRLKILKLLAEGELCVCELAEELGLSQSTVSHHLKKLREAGLV 45
COG3398 COG3398
Predicted transcriptional regulator, contains two HTH domains [Transcription];
17-97 3.88e-06

Predicted transcriptional regulator, contains two HTH domains [Transcription];


Pssm-ID: 442625 [Multi-domain]  Cd Length: 159  Bit Score: 42.94  E-value: 3.88e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489606730  17 PLAEMEERAVEVAILLKTlaHPARLMLACTLAQGEFSVGELEAKLDIRQPTLSQQLGVLREAGVVETRREAKQIFYRLTE 96
Cdd:COG3398   79 NSGTYSEEEKKILAYLRR--ETPRRILLYLLENPGATNKELAEELGISRSTVSWHLKRLEEDGLVERERDGRNVRYYLNP 156


                 .
gi 489606730  97 E 97
Cdd:COG3398  157 P 157
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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